tbc1d10b cDNA ORF clone, Poecilia reticulata(guppy)

Gene
Clones
gRNAs

Summary

Gene Symbol	tbc1d10b
Entrez Gene ID	103468933
Full Name	TBC1 domain family member 10B
Gene Type	protein-coding
Organism	Poecilia reticulata(guppy)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017306314.1	XP_017161803.1	TBC1 domain family member 10B
XM_008416328.2	XP_008414550.1	TBC1 domain family member 10B

Pathways from BioSystems
Gene Ontology

The following tbc1d10b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tbc1d10b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPo200249	XM_017306314.1 Latest version!	Poecilia reticulata TBC1 domain family member 10B (tbc1d10b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OPo200249	XM_008416328.2 Latest version!	Poecilia reticulata TBC1 domain family member 10B (tbc1d10b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tbc1d10b ( XM_017306314.1 ) cDNA ORF clone, Poecilia reticulata(guppy) -> XP_017161803.1 Poecilia reticulata TBC1 domain family member 10B (tbc1d10b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPo200249
Clone ID Related Accession (Same CDS sequence)	XM_017306314.1 , XM_008416328.2
Accession Version	XM_017306314.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2454bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-20
Organism	Poecilia reticulata(guppy)
Product	TBC1 domain family member 10B
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia reticulata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGAGC TGTCCACCTT GCCTCCTTCT CCACCTCCGC TGGGCGACCC GTGTGCCGCT 
CCAACTTCCA GCTGCCCCGA CTCTGTGAAT GAGCTGCCTC TGCCGAAGTC TTCCCTCTAC 
AACGACAAAG CTTTGACTGG AAACACTTGT TCAGCACTTT CAAGAGGTCC GACCTCGACT 
GCTCAACCTG AGACGGAGGA GCCAGCAGGG AAGAATCAGG AAACGGCGAC CGGCAACCCA 
TCTGTTGCAG AGTCCGGTTC TGACCAACCT GGGGCCCAGA TAAAAACCAC GCAGGAGCCG 
TTGTCTCCAG ATTTAAACCC CGGTCCCAAT CTGTATCCGA GCGTCCATGT TTCCTCTAAC 
CCGGACCCCG ACGTCGCTCA GCCGCAAACC AGTGCAGATT TCAACCCTGC AACATCATCG 
ACTGACGATG AGGAGAAACC TCAGCCGCCA CCGCAAGCAC AAAAACCTCA AAACGAGCCG 
CCGCCGCCGT CCCCAAACCT GGACCCGGTC GCTGAAGAGC TGCCGACTAC GTCCACCAGA 
GCGGCGCCGT CGAGCCCAAC TTTGCCTGTG ATCCAAGAGC CAGTCGGCCC GCTGTCGTAC 
CCCAAGCCAA AACCTGCGCC GGACACGCTA AGCTACCTAG ACTCGGCCAG CCTGATGTCG 
GGAACGCTGG AGTCGCTGTC CGGGTTGGGA GAGGACGGGA GCTCTGTGGG CTCAGACTCT 
GAGATCAACG GGCTCACCTT GAGACGCACC GATAAGTACG GGTTCCTGGG TGGCAATCAG 
TACAGCGACA CTGGTGAAAA GGAAGTTCGA GTTGAAGTTG CGAGGCAAAG GGAGATGAAG 
TGGCTGGACA TGTTCTGCCA CTGGGACAAG TGGGTCAAAC ATCGCTTCCA AAAGGTGAAG 
CTGCGCTGCA GGAAGGGAAT CCCGTCGTCC CTCAGATCCA AAGCCTGGCA GCTGCTGTCC 
GACAGCCAAG AGCTGCTGGA GGCAAACCCG GGAAAATTTG ATGAGCTGGA GAGAGAGCCG 
GGTGAGGCTA AATGGCTGGA CATCATCGAG AAAGATCTCC ACCGGCAGTT TCCTTTTCAC 
GAGATGTTTG CTGCGCGCGG CGGACACGGA CAGCAGGATT TGTACCGCAT CCTGAAGGCC 
TACACCGTGT ACCGGCCTGA TGAGGGCTAC TGCCAGGCCC AGGCTCCCGT GGCGGCGGTG 
CTGCTCATGC ACATGCCTGC AGAGCAAGCT TTCTGGTGCC TGGTCCAGAT TTGTGAGAAG 
TATCTGCCTG GCTACTACAG CGCCGGCCTG GAAGCGATTC AGCTGGACGG CGAGATCTTT 
TTCTCTCTCC TGCGGCGCAC GTGTCCCATG GCGTACCGCC ACCTCAAGAA ATTCAAGATT 
GACCCGATCC TCTACATGAC GGAGTGGTTC ATGTGCATAT TCTCACGCAC CTTACCGTGG 
TCCTCTGTGC TGCGTGTCTG GGACATGTTT TTCTGTGAAG GAGTGAAGAT AATATTTCGC 
GTGGGCTTGG TGCTGCTGAA GCAGATGCTG GGCTCGGTGG ATAAACTCCG GGAACTGCAG 
GGAATGTACG AAACCATGGA GCGGCTCAGG AACATCTCAC CCGACACCAT CCGGGAGGAC 
GTGCTGGTGC AGGAGATCAT AATGCTGCCG GTGACCGAGG CGCTCATCGA GAAGGAGTGC 
AGCGTCCAGG TGAGGAAGTG GAAGGAGTCC AGAGGTGAGC TGACCCACAA ACCGGGCCGC 
CGGCTCCACG GCACCAGAGC CATCTTGGAG TACAAGCGCC GGGCGTCGGC CATCAGCTCA 
GGCGGCAGCT TTTCCTTCCT GGGCAGCTCT GCGCCGCCGC CGGGGCCGCT GCGCGCTTCG 
TCCAGCCTGC TCTCCCTGCC CGGTTTCCGG AAATCCAGGG CTCCCTTCTT CTCCCAGCCT 
AAAAAAATCT CCTTCTCCGG TCCTTCTGGG GCGGAGAGTC AGTCGCCTGT CGCCTCATCG 
TCGGGCCAGA AGCCGCCGAT CCCTCCGGGC GCGGCTCCAA AGGCGACAAC GCCGCACGTC 
CAGAGCCCGT TAGCCGCGGA GAGCAGCTCA GCACAAATCC AGCCGGCTCC GCCAAACACT 
CTGTCGCCGA AGATCGTTTC AGAGCAGATC ACTCCCACAA TCCCATCGCC GACGGCCAAC 
AACGCGCCGC TGCAACCGCT GCCGCAACCG CAGCCGCAAC CGCAACCGCA ACCACCAAAA 
GAATCAACCC CATCTGTGTC CGGAGAGGAC GAAGGGAAGA AGAGGAAGAA AAGCAAAGAA 
GACAAGAAGA AGGAAAAGGA AGATGAGAAG AAAAAGCTGA AGGAGAAGAA AGACAAGGAG 
AAAGTTGAAA AGGAGAAAGC GAAGAAGGAA AAGGAGAAGA AGAAAGCGGG GAAGAAAAAG 
GACAAAGGGA CGGAGGAGAA CGGAGCGACG GCCGCGGCGA AAGATTCAAC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017161803.1
CDS	1..2454
Translation	MAELSTLPPSPPPLGDPCAAPTSSCPDSVNELPLPKSSLYNDKALTGNTCSALSRGPTSTAQPETEEPAGKNQETATGNPSVAESGSDQPGAQIKTTQEPLSPDLNPGPNLYPSVHVSSNPDPDVAQPQTSADFNPATSSTDDEEKPQPPPQAQKPQNEPPPPSPNLDPVAEELPTTSTRAAPSSPTLPVIQEPVGPLSYPKPKPAPDTLSYLDSASLMSGTLESLSGLGEDGSSVGSDSEINGLTLRRTDKYGFLGGNQYSDTGEKEVRVEVARQREMKWLDMFCHWDKWVKHRFQKVKLRCRKGIPSSLRSKAWQLLSDSQELLEANPGKFDELEREPGEAKWLDIIEKDLHRQFPFHEMFAARGGHGQQDLYRILKAYTVYRPDEGYCQAQAPVAAVLLMHMPAEQAFWCLVQICEKYLPGYYSAGLEAIQLDGEIFFSLLRRTCPMAYRHLKKFKIDPILYMTEWFMCIFSRTLPWSSVLRVWDMFFCEGVKIIFRVGLVLLKQMLGSVDKLRELQGMYETMERLRNISPDTIREDVLVQEIIMLPVTEALIEKECSVQVRKWKESRGELTHKPGRRLHGTRAILEYKRRASAISSGGSFSFLGSSAPPPGPLRASSSLLSLPGFRKSRAPFFSQPKKISFSGPSGAESQSPVASSSGQKPPIPPGAAPKATTPHVQSPLAAESSSAQIQPAPPNTLSPKIVSEQITPTIPSPTANNAPLQPLPQPQPQPQPQPPKESTPSVSGEDEGKKRKKSKEDKKKEKEDEKKKLKEKKDKEKVEKEKAKKEKEKKKAGKKKDKGTEENGATAAAKDST

Target ORF information:

RefSeq Version	XM_017306314.1
Organism	Poecilia reticulata(guppy)
Definition	Poecilia reticulata TBC1 domain family member 10B (tbc1d10b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017306314.1

ORF Insert Sequence:

ATGGCTGAGC TGTCCACCTT GCCTCCTTCT CCACCTCCGC TGGGCGACCC GTGTGCCGCT 
CCAACTTCCA GCTGCCCCGA CTCTGTGAAT GAGCTGCCTC TGCCGAAGTC TTCCCTCTAC 
AACGACAAAG CTTTGACTGG AAACACTTGT TCAGCACTTT CAAGAGGTCC GACCTCGACT 
GCTCAACCTG AGACGGAGGA GCCAGCAGGG AAGAATCAGG AAACGGCGAC CGGCAACCCA 
TCTGTTGCAG AGTCCGGTTC TGACCAACCT GGGGCCCAGA TAAAAACCAC GCAGGAGCCG 
TTGTCTCCAG ATTTAAACCC CGGTCCCAAT CTGTATCCGA GCGTCCATGT TTCCTCTAAC 
CCGGACCCCG ACGTCGCTCA GCCGCAAACC AGTGCAGATT TCAACCCTGC AACATCATCG 
ACTGACGATG AGGAGAAACC TCAGCCGCCA CCGCAAGCAC AAAAACCTCA AAACGAGCCG 
CCGCCGCCGT CCCCAAACCT GGACCCGGTC GCTGAAGAGC TGCCGACTAC GTCCACCAGA 
GCGGCGCCGT CGAGCCCAAC TTTGCCTGTG ATCCAAGAGC CAGTCGGCCC GCTGTCGTAC 
CCCAAGCCAA AACCTGCGCC GGACACGCTA AGCTACCTAG ACTCGGCCAG CCTGATGTCG 
GGAACGCTGG AGTCGCTGTC CGGGTTGGGA GAGGACGGGA GCTCTGTGGG CTCAGACTCT 
GAGATCAACG GGCTCACCTT GAGACGCACC GATAAGTACG GGTTCCTGGG TGGCAATCAG 
TACAGCGACA CTGGTGAAAA GGAAGTTCGA GTTGAAGTTG CGAGGCAAAG GGAGATGAAG 
TGGCTGGACA TGTTCTGCCA CTGGGACAAG TGGGTCAAAC ATCGCTTCCA AAAGGTGAAG 
CTGCGCTGCA GGAAGGGAAT CCCGTCGTCC CTCAGATCCA AAGCCTGGCA GCTGCTGTCC 
GACAGCCAAG AGCTGCTGGA GGCAAACCCG GGAAAATTTG ATGAGCTGGA GAGAGAGCCG 
GGTGAGGCTA AATGGCTGGA CATCATCGAG AAAGATCTCC ACCGGCAGTT TCCTTTTCAC 
GAGATGTTTG CTGCGCGCGG CGGACACGGA CAGCAGGATT TGTACCGCAT CCTGAAGGCC 
TACACCGTGT ACCGGCCTGA TGAGGGCTAC TGCCAGGCCC AGGCTCCCGT GGCGGCGGTG 
CTGCTCATGC ACATGCCTGC AGAGCAAGCT TTCTGGTGCC TGGTCCAGAT TTGTGAGAAG 
TATCTGCCTG GCTACTACAG CGCCGGCCTG GAAGCGATTC AGCTGGACGG CGAGATCTTT 
TTCTCTCTCC TGCGGCGCAC GTGTCCCATG GCGTACCGCC ACCTCAAGAA ATTCAAGATT 
GACCCGATCC TCTACATGAC GGAGTGGTTC ATGTGCATAT TCTCACGCAC CTTACCGTGG 
TCCTCTGTGC TGCGTGTCTG GGACATGTTT TTCTGTGAAG GAGTGAAGAT AATATTTCGC 
GTGGGCTTGG TGCTGCTGAA GCAGATGCTG GGCTCGGTGG ATAAACTCCG GGAACTGCAG 
GGAATGTACG AAACCATGGA GCGGCTCAGG AACATCTCAC CCGACACCAT CCGGGAGGAC 
GTGCTGGTGC AGGAGATCAT AATGCTGCCG GTGACCGAGG CGCTCATCGA GAAGGAGTGC 
AGCGTCCAGG TGAGGAAGTG GAAGGAGTCC AGAGGTGAGC TGACCCACAA ACCGGGCCGC 
CGGCTCCACG GCACCAGAGC CATCTTGGAG TACAAGCGCC GGGCGTCGGC CATCAGCTCA 
GGCGGCAGCT TTTCCTTCCT GGGCAGCTCT GCGCCGCCGC CGGGGCCGCT GCGCGCTTCG 
TCCAGCCTGC TCTCCCTGCC CGGTTTCCGG AAATCCAGGG CTCCCTTCTT CTCCCAGCCT 
AAAAAAATCT CCTTCTCCGG TCCTTCTGGG GCGGAGAGTC AGTCGCCTGT CGCCTCATCG 
TCGGGCCAGA AGCCGCCGAT CCCTCCGGGC GCGGCTCCAA AGGCGACAAC GCCGCACGTC 
CAGAGCCCGT TAGCCGCGGA GAGCAGCTCA GCACAAATCC AGCCGGCTCC GCCAAACACT 
CTGTCGCCGA AGATCGTTTC AGAGCAGATC ACTCCCACAA TCCCATCGCC GACGGCCAAC 
AACGCGCCGC TGCAACCGCT GCCGCAACCG CAGCCGCAAC CGCAACCGCA ACCACCAAAA 
GAATCAACCC CATCTGTGTC CGGAGAGGAC GAAGGGAAGA AGAGGAAGAA AAGCAAAGAA 
GACAAGAAGA AGGAAAAGGA AGATGAGAAG AAAAAGCTGA AGGAGAAGAA AGACAAGGAG 
AAAGTTGAAA AGGAGAAAGC GAAGAAGGAA AAGGAGAAGA AGAAAGCGGG GAAGAAAAAG 
GACAAAGGGA CGGAGGAGAA CGGAGCGACG GCCGCGGCGA AAGATTCAAC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tbc1d10b ( XM_008416328.2 ) cDNA ORF clone, Poecilia reticulata(guppy) -> XP_008414550.1 Poecilia reticulata TBC1 domain family member 10B (tbc1d10b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPo200249
Clone ID Related Accession (Same CDS sequence)	XM_017306314.1 , XM_008416328.2
Accession Version	XM_008416328.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2454bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-20
Organism	Poecilia reticulata(guppy)
Product	TBC1 domain family member 10B
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 21, 2016 this sequence version replaced XM_008416328.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia reticulata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGAGC TGTCCACCTT GCCTCCTTCT CCACCTCCGC TGGGCGACCC GTGTGCCGCT 
CCAACTTCCA GCTGCCCCGA CTCTGTGAAT GAGCTGCCTC TGCCGAAGTC TTCCCTCTAC 
AACGACAAAG CTTTGACTGG AAACACTTGT TCAGCACTTT CAAGAGGTCC GACCTCGACT 
GCTCAACCTG AGACGGAGGA GCCAGCAGGG AAGAATCAGG AAACGGCGAC CGGCAACCCA 
TCTGTTGCAG AGTCCGGTTC TGACCAACCT GGGGCCCAGA TAAAAACCAC GCAGGAGCCG 
TTGTCTCCAG ATTTAAACCC CGGTCCCAAT CTGTATCCGA GCGTCCATGT TTCCTCTAAC 
CCGGACCCCG ACGTCGCTCA GCCGCAAACC AGTGCAGATT TCAACCCTGC AACATCATCG 
ACTGACGATG AGGAGAAACC TCAGCCGCCA CCGCAAGCAC AAAAACCTCA AAACGAGCCG 
CCGCCGCCGT CCCCAAACCT GGACCCGGTC GCTGAAGAGC TGCCGACTAC GTCCACCAGA 
GCGGCGCCGT CGAGCCCAAC TTTGCCTGTG ATCCAAGAGC CAGTCGGCCC GCTGTCGTAC 
CCCAAGCCAA AACCTGCGCC GGACACGCTA AGCTACCTAG ACTCGGCCAG CCTGATGTCG 
GGAACGCTGG AGTCGCTGTC CGGGTTGGGA GAGGACGGGA GCTCTGTGGG CTCAGACTCT 
GAGATCAACG GGCTCACCTT GAGACGCACC GATAAGTACG GGTTCCTGGG TGGCAATCAG 
TACAGCGACA CTGGTGAAAA GGAAGTTCGA GTTGAAGTTG CGAGGCAAAG GGAGATGAAG 
TGGCTGGACA TGTTCTGCCA CTGGGACAAG TGGGTCAAAC ATCGCTTCCA AAAGGTGAAG 
CTGCGCTGCA GGAAGGGAAT CCCGTCGTCC CTCAGATCCA AAGCCTGGCA GCTGCTGTCC 
GACAGCCAAG AGCTGCTGGA GGCAAACCCG GGAAAATTTG ATGAGCTGGA GAGAGAGCCG 
GGTGAGGCTA AATGGCTGGA CATCATCGAG AAAGATCTCC ACCGGCAGTT TCCTTTTCAC 
GAGATGTTTG CTGCGCGCGG CGGACACGGA CAGCAGGATT TGTACCGCAT CCTGAAGGCC 
TACACCGTGT ACCGGCCTGA TGAGGGCTAC TGCCAGGCCC AGGCTCCCGT GGCGGCGGTG 
CTGCTCATGC ACATGCCTGC AGAGCAAGCT TTCTGGTGCC TGGTCCAGAT TTGTGAGAAG 
TATCTGCCTG GCTACTACAG CGCCGGCCTG GAAGCGATTC AGCTGGACGG CGAGATCTTT 
TTCTCTCTCC TGCGGCGCAC GTGTCCCATG GCGTACCGCC ACCTCAAGAA ATTCAAGATT 
GACCCGATCC TCTACATGAC GGAGTGGTTC ATGTGCATAT TCTCACGCAC CTTACCGTGG 
TCCTCTGTGC TGCGTGTCTG GGACATGTTT TTCTGTGAAG GAGTGAAGAT AATATTTCGC 
GTGGGCTTGG TGCTGCTGAA GCAGATGCTG GGCTCGGTGG ATAAACTCCG GGAACTGCAG 
GGAATGTACG AAACCATGGA GCGGCTCAGG AACATCTCAC CCGACACCAT CCGGGAGGAC 
GTGCTGGTGC AGGAGATCAT AATGCTGCCG GTGACCGAGG CGCTCATCGA GAAGGAGTGC 
AGCGTCCAGG TGAGGAAGTG GAAGGAGTCC AGAGGTGAGC TGACCCACAA ACCGGGCCGC 
CGGCTCCACG GCACCAGAGC CATCTTGGAG TACAAGCGCC GGGCGTCGGC CATCAGCTCA 
GGCGGCAGCT TTTCCTTCCT GGGCAGCTCT GCGCCGCCGC CGGGGCCGCT GCGCGCTTCG 
TCCAGCCTGC TCTCCCTGCC CGGTTTCCGG AAATCCAGGG CTCCCTTCTT CTCCCAGCCT 
AAAAAAATCT CCTTCTCCGG TCCTTCTGGG GCGGAGAGTC AGTCGCCTGT CGCCTCATCG 
TCGGGCCAGA AGCCGCCGAT CCCTCCGGGC GCGGCTCCAA AGGCGACAAC GCCGCACGTC 
CAGAGCCCGT TAGCCGCGGA GAGCAGCTCA GCACAAATCC AGCCGGCTCC GCCAAACACT 
CTGTCGCCGA AGATCGTTTC AGAGCAGATC ACTCCCACAA TCCCATCGCC GACGGCCAAC 
AACGCGCCGC TGCAACCGCT GCCGCAACCG CAGCCGCAAC CGCAACCGCA ACCACCAAAA 
GAATCAACCC CATCTGTGTC CGGAGAGGAC GAAGGGAAGA AGAGGAAGAA AAGCAAAGAA 
GACAAGAAGA AGGAAAAGGA AGATGAGAAG AAAAAGCTGA AGGAGAAGAA AGACAAGGAG 
AAAGTTGAAA AGGAGAAAGC GAAGAAGGAA AAGGAGAAGA AGAAAGCGGG GAAGAAAAAG 
GACAAAGGGA CGGAGGAGAA CGGAGCGACG GCCGCGGCGA AAGATTCAAC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008414550.1
CDS	456..2909
Translation	MAELSTLPPSPPPLGDPCAAPTSSCPDSVNELPLPKSSLYNDKALTGNTCSALSRGPTSTAQPETEEPAGKNQETATGNPSVAESGSDQPGAQIKTTQEPLSPDLNPGPNLYPSVHVSSNPDPDVAQPQTSADFNPATSSTDDEEKPQPPPQAQKPQNEPPPPSPNLDPVAEELPTTSTRAAPSSPTLPVIQEPVGPLSYPKPKPAPDTLSYLDSASLMSGTLESLSGLGEDGSSVGSDSEINGLTLRRTDKYGFLGGNQYSDTGEKEVRVEVARQREMKWLDMFCHWDKWVKHRFQKVKLRCRKGIPSSLRSKAWQLLSDSQELLEANPGKFDELEREPGEAKWLDIIEKDLHRQFPFHEMFAARGGHGQQDLYRILKAYTVYRPDEGYCQAQAPVAAVLLMHMPAEQAFWCLVQICEKYLPGYYSAGLEAIQLDGEIFFSLLRRTCPMAYRHLKKFKIDPILYMTEWFMCIFSRTLPWSSVLRVWDMFFCEGVKIIFRVGLVLLKQMLGSVDKLRELQGMYETMERLRNISPDTIREDVLVQEIIMLPVTEALIEKECSVQVRKWKESRGELTHKPGRRLHGTRAILEYKRRASAISSGGSFSFLGSSAPPPGPLRASSSLLSLPGFRKSRAPFFSQPKKISFSGPSGAESQSPVASSSGQKPPIPPGAAPKATTPHVQSPLAAESSSAQIQPAPPNTLSPKIVSEQITPTIPSPTANNAPLQPLPQPQPQPQPQPPKESTPSVSGEDEGKKRKKSKEDKKKEKEDEKKKLKEKKDKEKVEKEKAKKEKEKKKAGKKKDKGTEENGATAAAKDST

Target ORF information:

RefSeq Version	XM_008416328.2
Organism	Poecilia reticulata(guppy)
Definition	Poecilia reticulata TBC1 domain family member 10B (tbc1d10b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008416328.2

ORF Insert Sequence:

ATGGCTGAGC TGTCCACCTT GCCTCCTTCT CCACCTCCGC TGGGCGACCC GTGTGCCGCT 
CCAACTTCCA GCTGCCCCGA CTCTGTGAAT GAGCTGCCTC TGCCGAAGTC TTCCCTCTAC 
AACGACAAAG CTTTGACTGG AAACACTTGT TCAGCACTTT CAAGAGGTCC GACCTCGACT 
GCTCAACCTG AGACGGAGGA GCCAGCAGGG AAGAATCAGG AAACGGCGAC CGGCAACCCA 
TCTGTTGCAG AGTCCGGTTC TGACCAACCT GGGGCCCAGA TAAAAACCAC GCAGGAGCCG 
TTGTCTCCAG ATTTAAACCC CGGTCCCAAT CTGTATCCGA GCGTCCATGT TTCCTCTAAC 
CCGGACCCCG ACGTCGCTCA GCCGCAAACC AGTGCAGATT TCAACCCTGC AACATCATCG 
ACTGACGATG AGGAGAAACC TCAGCCGCCA CCGCAAGCAC AAAAACCTCA AAACGAGCCG 
CCGCCGCCGT CCCCAAACCT GGACCCGGTC GCTGAAGAGC TGCCGACTAC GTCCACCAGA 
GCGGCGCCGT CGAGCCCAAC TTTGCCTGTG ATCCAAGAGC CAGTCGGCCC GCTGTCGTAC 
CCCAAGCCAA AACCTGCGCC GGACACGCTA AGCTACCTAG ACTCGGCCAG CCTGATGTCG 
GGAACGCTGG AGTCGCTGTC CGGGTTGGGA GAGGACGGGA GCTCTGTGGG CTCAGACTCT 
GAGATCAACG GGCTCACCTT GAGACGCACC GATAAGTACG GGTTCCTGGG TGGCAATCAG 
TACAGCGACA CTGGTGAAAA GGAAGTTCGA GTTGAAGTTG CGAGGCAAAG GGAGATGAAG 
TGGCTGGACA TGTTCTGCCA CTGGGACAAG TGGGTCAAAC ATCGCTTCCA AAAGGTGAAG 
CTGCGCTGCA GGAAGGGAAT CCCGTCGTCC CTCAGATCCA AAGCCTGGCA GCTGCTGTCC 
GACAGCCAAG AGCTGCTGGA GGCAAACCCG GGAAAATTTG ATGAGCTGGA GAGAGAGCCG 
GGTGAGGCTA AATGGCTGGA CATCATCGAG AAAGATCTCC ACCGGCAGTT TCCTTTTCAC 
GAGATGTTTG CTGCGCGCGG CGGACACGGA CAGCAGGATT TGTACCGCAT CCTGAAGGCC 
TACACCGTGT ACCGGCCTGA TGAGGGCTAC TGCCAGGCCC AGGCTCCCGT GGCGGCGGTG 
CTGCTCATGC ACATGCCTGC AGAGCAAGCT TTCTGGTGCC TGGTCCAGAT TTGTGAGAAG 
TATCTGCCTG GCTACTACAG CGCCGGCCTG GAAGCGATTC AGCTGGACGG CGAGATCTTT 
TTCTCTCTCC TGCGGCGCAC GTGTCCCATG GCGTACCGCC ACCTCAAGAA ATTCAAGATT 
GACCCGATCC TCTACATGAC GGAGTGGTTC ATGTGCATAT TCTCACGCAC CTTACCGTGG 
TCCTCTGTGC TGCGTGTCTG GGACATGTTT TTCTGTGAAG GAGTGAAGAT AATATTTCGC 
GTGGGCTTGG TGCTGCTGAA GCAGATGCTG GGCTCGGTGG ATAAACTCCG GGAACTGCAG 
GGAATGTACG AAACCATGGA GCGGCTCAGG AACATCTCAC CCGACACCAT CCGGGAGGAC 
GTGCTGGTGC AGGAGATCAT AATGCTGCCG GTGACCGAGG CGCTCATCGA GAAGGAGTGC 
AGCGTCCAGG TGAGGAAGTG GAAGGAGTCC AGAGGTGAGC TGACCCACAA ACCGGGCCGC 
CGGCTCCACG GCACCAGAGC CATCTTGGAG TACAAGCGCC GGGCGTCGGC CATCAGCTCA 
GGCGGCAGCT TTTCCTTCCT GGGCAGCTCT GCGCCGCCGC CGGGGCCGCT GCGCGCTTCG 
TCCAGCCTGC TCTCCCTGCC CGGTTTCCGG AAATCCAGGG CTCCCTTCTT CTCCCAGCCT 
AAAAAAATCT CCTTCTCCGG TCCTTCTGGG GCGGAGAGTC AGTCGCCTGT CGCCTCATCG 
TCGGGCCAGA AGCCGCCGAT CCCTCCGGGC GCGGCTCCAA AGGCGACAAC GCCGCACGTC 
CAGAGCCCGT TAGCCGCGGA GAGCAGCTCA GCACAAATCC AGCCGGCTCC GCCAAACACT 
CTGTCGCCGA AGATCGTTTC AGAGCAGATC ACTCCCACAA TCCCATCGCC GACGGCCAAC 
AACGCGCCGC TGCAACCGCT GCCGCAACCG CAGCCGCAAC CGCAACCGCA ACCACCAAAA 
GAATCAACCC CATCTGTGTC CGGAGAGGAC GAAGGGAAGA AGAGGAAGAA AAGCAAAGAA 
GACAAGAAGA AGGAAAAGGA AGATGAGAAG AAAAAGCTGA AGGAGAAGAA AGACAAGGAG 
AAAGTTGAAA AGGAGAAAGC GAAGAAGGAA AAGGAGAAGA AGAAAGCGGG GAAGAAAAAG 
GACAAAGGGA CGGAGGAGAA CGGAGCGACG GCCGCGGCGA AAGATTCAAC CTAG