lemd3 cDNA ORF clone, Poecilia reticulata(guppy)

Gene
Clones
gRNAs

Summary

Gene Symbol	lemd3
Entrez Gene ID	103459738
Full Name	LEM domain containing 3
Gene Type	protein-coding
Organism	Poecilia reticulata(guppy)

mRNA and Protein(s)

mRNA	Protein	Name
XM_008401549.2	XP_008399771.1	inner nuclear membrane protein Man1

Pathways from BioSystems
Gene Ontology

The following lemd3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the lemd3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPo190513	XM_008401549.2 Latest version!	Poecilia reticulata LEM domain containing 3 (lemd3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

lemd3 ( XM_008401549.2 ) cDNA ORF clone, Poecilia reticulata(guppy) -> XP_008399771.1 Poecilia reticulata LEM domain containing 3 (lemd3), mRNA.

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CloneID	OPo190513
Clone ID Related Accession (Same CDS sequence)	XM_008401549.2
Accession Version	XM_008401549.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2502bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-20
Organism	Poecilia reticulata(guppy)
Product	inner nuclear membrane protein Man1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024353.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 21, 2016 this sequence version replaced XM_008401549.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia reticulata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCGA CGCAGTTAAC AGATGAGGAG CTTTTTTCCC AGTTGAAGTG CTTCGGTTAC 
ACTCCTGGCC CAGTCACAGA AAACACACGT CCGGTGTATT TAAAGAAACT GAAGAAGTTT 
CGCGAGGAGC AGCAGCAGCA GCAGCGGGGT AAAGGCCGAG TCAGCGGGAC GAGCAGCAGC 
AGCAGCACTG GGGCACCAAG GCCAGTTAGA CATGACGTCA GGCGCCTGAG CTCCGGTAGG 
AGACCGGGCC TAAAGTCCTC TGTTCTCGGC TTCAGCTCTG ACGAATCTGA CGCGGAAACG 
CCACTGAAAA GAAAGAACCT GGATCACATG AAAAGACCCA GCCGAAGCCC ACACCTTCAA 
CAACAACAAC AGCTGAAGAT TAGAGCAGCT ACGCCTCTTA ACGAGGCTAA CAAGAAGCAG 
TATGGCGTTG GGACTGCTCT GAAAAGCAAT TCATTCCCGA ACCCAGACGG ACAGACAAGT 
GCCTCGCTGG GTTGGGAAAC TGGTAAAAGA TCTATGCATG ATCCTGAATC CAGGGACGAA 
GACGTTAGTG ATGAAGTGCA GAATTCCCAC CCGGTTAATG GTCGCAGCGC CGCTCGCCTG 
AACACTAGCA AACTAGCCGG AGAGTACTCA GACTCGGACG AGGAGGAAGC TGGGGGCCCT 
GGAGGCCCAG AGCGAGACCG AAGGTGTTTG GAGCTGCGAC GGATTTACAC AAGCGGACCA 
TTTTCTTCGC ATGTAGACGG CGGAGGGTCC CGGACCGGAG GGAAAAACTG TACGCTTAAT 
CCGCTTGGGA ACAGAGCCAT GGCAGGCGGC AGAGAGGAGT ATGAGGAGAG AGGGAAGAAA 
GGAGACCTGG ACGCATCGGG AGGCTTACGG AGCCACATGC TTCCCAGGAA GCCGATCTAC 
GTGTCCCTCG GCGAGGACCA CCAGAGTAAG ACCGGAGAAA ACAACCATAT TAACAGCGAG 
GAGGGGGCGT CCGGCAGCAA CATGGCCAGC GTCGCGCTGA GACCGCGCTA CCGCGGTTCC 
TACAGCCCGA CCCTGAGCCC TCAGGGGGGC TACTCTAATC ACAGCCCCGT CCCGAACCAC 
GCATACGGCC CGGGAACCCA GAAGAAGAAG CCAACTGCCC CAGAGGATGA GCTGCTGCTG 
CAGTTTAAAA GAGAGGAGCA ACCTTCCTCC GGAAGCTTCA GCGCGCACTA CCTGTCCATG 
TTCCTGCTCA CCGCAGCCTG CCTCTTTTTC CTCCTGCTCG GCCTCATGTA CATCCGCATG 
AGGGGCTCGG GATCCCCGGC GGGGGATGGT GTCATTAAGA GTCACCCGTT TGGCAGCAAC 
TTTGACTCCT CATATAGTAA GACAGAAAAA GACGTGATCC TAAAGCTGCT GCTCAGCCTC 
CATGATCACC TGGCCATCGT TGCTGGTCAT CATGACTGTG GAGAGCAGCA GTATGCTAGC 
AGGAGTCTGT CCAGAGAGCA GGTCACACAT TATTTACTGG CTCAGGATGG AGACTTTAAA 
GACTTCATTG ACGTCTCTCT AGAGTGGATC ATTCGAGCTG GCCAGGATGT CGGTATAAGG 
TTGAGCGGGG AGCTAGCTGA CGACCTAGTG ACGGATGTGT CTGAGATCTC TTGGTTGGAA 
TCCACGCATC CCAGAATGCC GTTCACCTGC CGCTTTCGTC GAGCTTTGCT CACAGTCATG 
AACAAAGTCC TGGTCGTTGC CGTTGTTGTA GGCTTAGTGT GCAGTGTGAT CTGCTACGTG 
AAGTATCGCT GGAGGAGAGA GGAGGAGGAG ACGAGGCAGA TGTACGACAT GGTGGAGAGG 
ATCATCGATG TGTTAAGGAC CCACGGTGAG GCATGCCAAG AGAACCGGGA CCTAGAGCCT 
TACCTGCCCA TCCCCCATGT CAGAGACTCC CTGGTTCAAC CCCAGGATAG GAGAAAGATG 
AAGAAGGTTT GGGAGCGAGC CGTGAAGTTC CTCTCAGCTA ACGAGTCCAG GATCCGGACA 
GAGACACAGC AGATCAAGGG AGCAGACTTC ATGGTGTGGA GGTGGATCCA GCCGCCCCTC 
AGCTCTGACA AATCCAAGAT GTGGCAGGGG AAAGCATTCC CTCTAGATCG GAGGAATTCA 
CCGCCCAACA GTCTGACTCC ATGTCTGAAG ATCAGAAACA TGTTTGATCC AGACATGGAG 
GTTGGTGACA GCTGGGACTT GGCCATCCAG GAAGCCATCC TGGAGAAATG CAGTGACAAT 
GACGGCATCG TTCATATTTC TGTTGATAAG AACTCTAAAG AGGGCTGCGT CTATGTCAAG 
TGTCTTTCTG CAGAGCACTC AGGAAAAGCC TTCAAAGCCC TGCATGGCTC CTGGTTTGAT 
GGTAAGCTGG TGACAGTCAA GTATCTGCGT CTGGACCGGT ACCACCAGCG CTTCCCGCAG 
GCCCAGGGCT GCAGTGCGCC CCTGAAGGCG TACAGCCTCC ACCAGACCCC GGCTCCCTGC 
CCACTGCAGC ATGGGGGCCC CAACTCCTCA GGCTTCTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008399771.1
CDS	36..2537
Translation	MASTQLTDEELFSQLKCFGYTPGPVTENTRPVYLKKLKKFREEQQQQQRGKGRVSGTSSSSSTGAPRPVRHDVRRLSSGRRPGLKSSVLGFSSDESDAETPLKRKNLDHMKRPSRSPHLQQQQQLKIRAATPLNEANKKQYGVGTALKSNSFPNPDGQTSASLGWETGKRSMHDPESRDEDVSDEVQNSHPVNGRSAARLNTSKLAGEYSDSDEEEAGGPGGPERDRRCLELRRIYTSGPFSSHVDGGGSRTGGKNCTLNPLGNRAMAGGREEYEERGKKGDLDASGGLRSHMLPRKPIYVSLGEDHQSKTGENNHINSEEGASGSNMASVALRPRYRGSYSPTLSPQGGYSNHSPVPNHAYGPGTQKKKPTAPEDELLLQFKREEQPSSGSFSAHYLSMFLLTAACLFFLLLGLMYIRMRGSGSPAGDGVIKSHPFGSNFDSSYSKTEKDVILKLLLSLHDHLAIVAGHHDCGEQQYASRSLSREQVTHYLLAQDGDFKDFIDVSLEWIIRAGQDVGIRLSGELADDLVTDVSEISWLESTHPRMPFTCRFRRALLTVMNKVLVVAVVVGLVCSVICYVKYRWRREEEETRQMYDMVERIIDVLRTHGEACQENRDLEPYLPIPHVRDSLVQPQDRRKMKKVWERAVKFLSANESRIRTETQQIKGADFMVWRWIQPPLSSDKSKMWQGKAFPLDRRNSPPNSLTPCLKIRNMFDPDMEVGDSWDLAIQEAILEKCSDNDGIVHISVDKNSKEGCVYVKCLSAEHSGKAFKALHGSWFDGKLVTVKYLRLDRYHQRFPQAQGCSAPLKAYSLHQTPAPCPLQHGGPNSSGFS

Target ORF information:

RefSeq Version	XM_008401549.2
Organism	Poecilia reticulata(guppy)
Definition	Poecilia reticulata LEM domain containing 3 (lemd3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008401549.2

ORF Insert Sequence:

ATGGCGTCGA CGCAGTTAAC AGATGAGGAG CTTTTTTCCC AGTTGAAGTG CTTCGGTTAC 
ACTCCTGGCC CAGTCACAGA AAACACACGT CCGGTGTATT TAAAGAAACT GAAGAAGTTT 
CGCGAGGAGC AGCAGCAGCA GCAGCGGGGT AAAGGCCGAG TCAGCGGGAC GAGCAGCAGC 
AGCAGCACTG GGGCACCAAG GCCAGTTAGA CATGACGTCA GGCGCCTGAG CTCCGGTAGG 
AGACCGGGCC TAAAGTCCTC TGTTCTCGGC TTCAGCTCTG ACGAATCTGA CGCGGAAACG 
CCACTGAAAA GAAAGAACCT GGATCACATG AAAAGACCCA GCCGAAGCCC ACACCTTCAA 
CAACAACAAC AGCTGAAGAT TAGAGCAGCT ACGCCTCTTA ACGAGGCTAA CAAGAAGCAG 
TATGGCGTTG GGACTGCTCT GAAAAGCAAT TCATTCCCGA ACCCAGACGG ACAGACAAGT 
GCCTCGCTGG GTTGGGAAAC TGGTAAAAGA TCTATGCATG ATCCTGAATC CAGGGACGAA 
GACGTTAGTG ATGAAGTGCA GAATTCCCAC CCGGTTAATG GTCGCAGCGC CGCTCGCCTG 
AACACTAGCA AACTAGCCGG AGAGTACTCA GACTCGGACG AGGAGGAAGC TGGGGGCCCT 
GGAGGCCCAG AGCGAGACCG AAGGTGTTTG GAGCTGCGAC GGATTTACAC AAGCGGACCA 
TTTTCTTCGC ATGTAGACGG CGGAGGGTCC CGGACCGGAG GGAAAAACTG TACGCTTAAT 
CCGCTTGGGA ACAGAGCCAT GGCAGGCGGC AGAGAGGAGT ATGAGGAGAG AGGGAAGAAA 
GGAGACCTGG ACGCATCGGG AGGCTTACGG AGCCACATGC TTCCCAGGAA GCCGATCTAC 
GTGTCCCTCG GCGAGGACCA CCAGAGTAAG ACCGGAGAAA ACAACCATAT TAACAGCGAG 
GAGGGGGCGT CCGGCAGCAA CATGGCCAGC GTCGCGCTGA GACCGCGCTA CCGCGGTTCC 
TACAGCCCGA CCCTGAGCCC TCAGGGGGGC TACTCTAATC ACAGCCCCGT CCCGAACCAC 
GCATACGGCC CGGGAACCCA GAAGAAGAAG CCAACTGCCC CAGAGGATGA GCTGCTGCTG 
CAGTTTAAAA GAGAGGAGCA ACCTTCCTCC GGAAGCTTCA GCGCGCACTA CCTGTCCATG 
TTCCTGCTCA CCGCAGCCTG CCTCTTTTTC CTCCTGCTCG GCCTCATGTA CATCCGCATG 
AGGGGCTCGG GATCCCCGGC GGGGGATGGT GTCATTAAGA GTCACCCGTT TGGCAGCAAC 
TTTGACTCCT CATATAGTAA GACAGAAAAA GACGTGATCC TAAAGCTGCT GCTCAGCCTC 
CATGATCACC TGGCCATCGT TGCTGGTCAT CATGACTGTG GAGAGCAGCA GTATGCTAGC 
AGGAGTCTGT CCAGAGAGCA GGTCACACAT TATTTACTGG CTCAGGATGG AGACTTTAAA 
GACTTCATTG ACGTCTCTCT AGAGTGGATC ATTCGAGCTG GCCAGGATGT CGGTATAAGG 
TTGAGCGGGG AGCTAGCTGA CGACCTAGTG ACGGATGTGT CTGAGATCTC TTGGTTGGAA 
TCCACGCATC CCAGAATGCC GTTCACCTGC CGCTTTCGTC GAGCTTTGCT CACAGTCATG 
AACAAAGTCC TGGTCGTTGC CGTTGTTGTA GGCTTAGTGT GCAGTGTGAT CTGCTACGTG 
AAGTATCGCT GGAGGAGAGA GGAGGAGGAG ACGAGGCAGA TGTACGACAT GGTGGAGAGG 
ATCATCGATG TGTTAAGGAC CCACGGTGAG GCATGCCAAG AGAACCGGGA CCTAGAGCCT 
TACCTGCCCA TCCCCCATGT CAGAGACTCC CTGGTTCAAC CCCAGGATAG GAGAAAGATG 
AAGAAGGTTT GGGAGCGAGC CGTGAAGTTC CTCTCAGCTA ACGAGTCCAG GATCCGGACA 
GAGACACAGC AGATCAAGGG AGCAGACTTC ATGGTGTGGA GGTGGATCCA GCCGCCCCTC 
AGCTCTGACA AATCCAAGAT GTGGCAGGGG AAAGCATTCC CTCTAGATCG GAGGAATTCA 
CCGCCCAACA GTCTGACTCC ATGTCTGAAG ATCAGAAACA TGTTTGATCC AGACATGGAG 
GTTGGTGACA GCTGGGACTT GGCCATCCAG GAAGCCATCC TGGAGAAATG CAGTGACAAT 
GACGGCATCG TTCATATTTC TGTTGATAAG AACTCTAAAG AGGGCTGCGT CTATGTCAAG 
TGTCTTTCTG CAGAGCACTC AGGAAAAGCC TTCAAAGCCC TGCATGGCTC CTGGTTTGAT 
GGTAAGCTGG TGACAGTCAA GTATCTGCGT CTGGACCGGT ACCACCAGCG CTTCCCGCAG 
GCCCAGGGCT GCAGTGCGCC CCTGAAGGCG TACAGCCTCC ACCAGACCCC GGCTCCCTGC 
CCACTGCAGC ATGGGGGCCC CAACTCCTCA GGCTTCTCCT GA