actb cDNA ORF clone, Poecilia mexicana

Gene
Clones
gRNAs

Summary

Gene Symbol	actb
Entrez Gene ID	106927995
Full Name	actin beta
Synonyms	beta-actin
Gene Type	protein-coding
Organism	Poecilia mexicana

mRNA and Protein(s)

mRNA	Protein	Name
XM_015003955.1	XP_014859441.1	actin, cytoplasmic 1

Pathways from BioSystems
Gene Ontology

The following actb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the actb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPo69810	XM_015003955.1 Latest version!	Poecilia mexicana actin, beta (actb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

actb ( XM_015003955.1 ) cDNA ORF clone, Poecilia mexicana -> XP_014859441.1 Poecilia mexicana actin, beta (actb), mRNA.

Price & Availability↑

CloneID	OPo69810
Clone ID Related Accession (Same CDS sequence)	XM_015003955.1
Accession Version	XM_015003955.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1128bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-14
Organism	Poecilia mexicana
Product	actin, cytoplasmic 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015095505.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia mexicana Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGATG AAATCGCCGC ACTGGTTGTT GACAACGGAT CCGGTATGTG CAAAGCCGGA 
TTCGCTGGAG ACGACGCCCC TCGTGCTGTC TTCCCATCCA TCGTTGGTCG CCCAAGGCAT 
CAGGGCGTGA TGGTTGGTAT GGGCCAGAAG GACAGCTACG TAGGTGATGA AGCCCAGAGC 
AAGAGGGGTA TCCTGACCCT GAAGTACCCC ATCGAGCACG GTATTGTGAC CAACTGGGAT 
GACATGGAGA AGATCTGGCA TCACACCTTC TACAACGAGC TGAGAGTTGC CCCTGAGGAG 
CACCCCGTCC TGCTCACAGA GGCCCCTCTG AACCCCAAAG CCAACAGGGA GAAGATGACC 
CAGATCATGT TTGAGACCTT CAACACCCCC GCCATGTACG TTGCCATCCA GGCCGTGCTG 
TCCCTGTACG CTTCTGGTCG TACCACTGGT ATCGTCATGG ACTCCGGTGA TGGTGTGACC 
CACACAGTGC CCATCTATGA GGGCTACGCC CTGCCCCATG CCATCCTGCG TCTGGACTTG 
GCCGGCCGCG ACCTCACAGA CTACCTCATG AAGATCCTGA CAGAGCGTGG CTATTCCTTC 
ACCACCACAG CTGAGAGGGA AATTGTGCGT GACATCAAGG AGAAGCTGTG CTACGTCGCC 
CTGGACTTCG AGCAGGAGAT GGGTACTGCT GCCTCCTCTT CATCCCTGGA GAAGAGCTAC 
GAGCTCCCTG ACGGACAGGT CATCACCATT GGCAATGAGA GGTTCCGTTG CCCAGAGGCC 
CTCTTCCAGC CTTCCTTCCT TGGTATGGAG TCCTGCGGAA TCCACGAGAC CACCTACAAC 
AGCATCATGA AGTGCGACGT CGACATCCGT AAGGACCTGT ACGCCAACAC CGTGCTGTCT 
GGAGGTACCA CCATGTACCC TGGCATCGCC GACAGGATGC AGAAGGAGAT CACAGCCCTG 
GCCCCATCCA CCATGAAGAT CAAGATCATC GCCCCACCAG AGCGTAAATA CTCTGTCTGG 
ATCGGAGGCT CCATCCTGGC CTCCCTGTCC ACCTTCCAGC AGATGTGGAT CAGCAAGCAG 
GAGTACGATG AGTCTGGCCC CTCCATCGTC CACCGCAAAT GCTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014859441.1
CDS	131..1258
Translation	MEDEIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMGTAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF

Target ORF information:

RefSeq Version	XM_015003955.1
Organism	Poecilia mexicana
Definition	Poecilia mexicana actin, beta (actb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015003955.1

ORF Insert Sequence:

ATGGAAGATG AAATCGCCGC ACTGGTTGTT GACAACGGAT CCGGTATGTG CAAAGCCGGA 
TTCGCTGGAG ACGACGCCCC TCGTGCTGTC TTCCCATCCA TCGTTGGTCG CCCAAGGCAT 
CAGGGCGTGA TGGTTGGTAT GGGCCAGAAG GACAGCTACG TAGGTGATGA AGCCCAGAGC 
AAGAGGGGTA TCCTGACCCT GAAGTACCCC ATCGAGCACG GTATTGTGAC CAACTGGGAT 
GACATGGAGA AGATCTGGCA TCACACCTTC TACAACGAGC TGAGAGTTGC CCCTGAGGAG 
CACCCCGTCC TGCTCACAGA GGCCCCTCTG AACCCCAAAG CCAACAGGGA GAAGATGACC 
CAGATCATGT TTGAGACCTT CAACACCCCC GCCATGTACG TTGCCATCCA GGCCGTGCTG 
TCCCTGTACG CTTCTGGTCG TACCACTGGT ATCGTCATGG ACTCCGGTGA TGGTGTGACC 
CACACAGTGC CCATCTATGA GGGCTACGCC CTGCCCCATG CCATCCTGCG TCTGGACTTG 
GCCGGCCGCG ACCTCACAGA CTACCTCATG AAGATCCTGA CAGAGCGTGG CTATTCCTTC 
ACCACCACAG CTGAGAGGGA AATTGTGCGT GACATCAAGG AGAAGCTGTG CTACGTCGCC 
CTGGACTTCG AGCAGGAGAT GGGTACTGCT GCCTCCTCTT CATCCCTGGA GAAGAGCTAC 
GAGCTCCCTG ACGGACAGGT CATCACCATT GGCAATGAGA GGTTCCGTTG CCCAGAGGCC 
CTCTTCCAGC CTTCCTTCCT TGGTATGGAG TCCTGCGGAA TCCACGAGAC CACCTACAAC 
AGCATCATGA AGTGCGACGT CGACATCCGT AAGGACCTGT ACGCCAACAC CGTGCTGTCT 
GGAGGTACCA CCATGTACCC TGGCATCGCC GACAGGATGC AGAAGGAGAT CACAGCCCTG 
GCCCCATCCA CCATGAAGAT CAAGATCATC GCCCCACCAG AGCGTAAATA CTCTGTCTGG 
ATCGGAGGCT CCATCCTGGC CTCCCTGTCC ACCTTCCAGC AGATGTGGAT CAGCAAGCAG 
GAGTACGATG AGTCTGGCCC CTCCATCGTC CACCGCAAAT GCTTCTAA