LOC106906933 cDNA ORF clone, Poecilia mexicana

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC106906933
Entrez Gene ID	106906933
Full Name	E3 ubiquitin/ISG15 ligase TRIM25-like
Gene Type	protein-coding
Organism	Poecilia mexicana

mRNA and Protein(s)

mRNA	Protein	Name
XM_014972407.1	XP_014827893.1	E3 ubiquitin/ISG15 ligase TRIM25-like

Pathways from BioSystems
Gene Ontology

The following LOC106906933 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC106906933 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPo93686	XM_014972407.1 Latest version!	Poecilia mexicana E3 ubiquitin/ISG15 ligase TRIM25-like (LOC106906933), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC106906933 ( XM_014972407.1 ) cDNA ORF clone, Poecilia mexicana -> XP_014827893.1 Poecilia mexicana E3 ubiquitin/ISG15 ligase TRIM25-like (LOC106906933), mRNA.

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CloneID	OPo93686
Clone ID Related Accession (Same CDS sequence)	XM_014972407.1
Accession Version	XM_014972407.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1746bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-14
Organism	Poecilia mexicana
Product	E3 ubiquitin/ISG15 ligase TRIM25-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015096973.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia mexicana Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTTT TGTCAAGAAG AGAGACAAGA ATAAAGAAAG AAACTACAAC CAGAAGAGAA 
AAAAAACTGC AATTCTTTTC AACTTTCAGT ATGTTTTTAT CAAAGCAAGA GGACCAGCTT 
GCCCAGGAGC TAAGCTGTCC CATCTGTCTT CAGCTCTACA ACGATCCAGT TGTTCTACCC 
TGTGGGCACA ACTACTGTCG AGCTTGCATC TCTAAGTCTG CTGACTCCAT AGACAGTGGC 
AAAGAACCTC TACGCTGCCC AGAGTGTCGC GAGGCCTATC ATGGGGTCGA TACTCTGCAG 
AAAAATTTCA AACTTTCCAA CATTGTCGAG GGTTTCCAAG CTTCTTCCCA AAACATTTAC 
GAAGAGATCC AGGATGTGAT CAAACCAACG AACCCAGACG CGGATGATGT CATTTTCTGT 
GACCAATGCA TAGATGACAA GACTCCGGCA GTGAAGACCT GTCTGAAGTG TGAGGTGTCG 
CTGTGCTTCA GGCATCTCCA GAAGCATAAC GAGAGGGAGT CCTTCAAGAC ACACTCTCTG 
GTGGAACCTC TGAAGGATTT GGGGAGCAGG GCCTGCCCCG TCCATAACCT TCCACTTGAG 
TACTTTTGTT CTAATGACAT GAGGTCTCTG TGCATCACAT GTTTGGAAGG CCATGACCAG 
AACCACGACG TCCTCACCTT CAGTGTGGCA GAGGAGGAGA TGAGACGAGC CTTGGAGAGC 
CGTAGCAAGG TGGTTTCCAG CCGACTGCAA CTGACAGAGT GCCTCCTTCA GAAGACACAA 
GAGGACCAGG GAGCCTCCGA GGCTGTTGGA GATAAAATGG TCAACAAGGC AGTCTCACTG 
ATGGATAGTA TGGCTGCAAT AGTGGACAGG TTCAGGGAGC GATTGCGTTT GCTTTTGGAG 
GAGGAGAGAG GGCAGCGGCG CAAAAGCTGG CAGCTTGGAG TGAGTGCACT GGAGGAGCAG 
CAGCAGCAGC TGCAGGAGGC CCAGAAAAGT GCATCCGAGG CGCTCAGCGA GACAGACACA 
TGCGCCTTCA TACACAGATT CATGATGATT GAACAGAAAC TGAGAGATGC TGCCACAGGC 
AGTCTTCCCA GCACGATTCC CTCAAAGGTC GACCTGAACA CCAAGCGACT CCAGACAGAC 
CTCAAAACCA ACGACTTCCG CACAGAAATA GTCCGTCTCC TTGACACCCT TCACATCCTG 
CTCAATCCTC TGGAGGTCAC CTTCAACGTC TGTACTGCCC ACCCCAGCTT GTTGGTGTCC 
AACGACCTGC ACACAGTCAG ATGCACCAGC TCAAAGCAGT CCTACGTAGA GCACCCGGAG 
CGCTTCCTTA GTGCACCTCA GATCCTGTGC AGCCAGGGCT TTTCCAGAGG AACACATATC 
TGGGTGGCAG AAATGGGCAG CAACTGCATG TGGTCCCTTG GCGCATGCTA CAAGAGCATC 
CCTCGCCGTG GAGACCACAG CCGCCTGGGG CACAACTTGG TGTCCTGGAG ACTACAGTGG 
AAAAATGGCA AGCTAACTGC GTGCTCTTCA TCTAGCAATG CTATGCTGGG GGAGGTGTTC 
CAATATCCAT CTAAGATTGA GGTTGTGCTT GACTGTGAAG GTGGAACCCT GTCCTTCCAC 
ACCATCAAAT CGCGCAGAGA ACACCTTTAC ACCTTTAAGA CTGTGTTTAA GGAACCTGTT 
TACCCAGCTT TTAGCATACA TTCAAATACA TCAGAGTCTT GGATCACTCT ACATAGTCAG 
ATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014827893.1
CDS	84..1829
Translation	MKVLSRRETRIKKETTTRREKKLQFFSTFSMFLSKQEDQLAQELSCPICLQLYNDPVVLPCGHNYCRACISKSADSIDSGKEPLRCPECREAYHGVDTLQKNFKLSNIVEGFQASSQNIYEEIQDVIKPTNPDADDVIFCDQCIDDKTPAVKTCLKCEVSLCFRHLQKHNERESFKTHSLVEPLKDLGSRACPVHNLPLEYFCSNDMRSLCITCLEGHDQNHDVLTFSVAEEEMRRALESRSKVVSSRLQLTECLLQKTQEDQGASEAVGDKMVNKAVSLMDSMAAIVDRFRERLRLLLEEERGQRRKSWQLGVSALEEQQQQLQEAQKSASEALSETDTCAFIHRFMMIEQKLRDAATGSLPSTIPSKVDLNTKRLQTDLKTNDFRTEIVRLLDTLHILLNPLEVTFNVCTAHPSLLVSNDLHTVRCTSSKQSYVEHPERFLSAPQILCSQGFSRGTHIWVAEMGSNCMWSLGACYKSIPRRGDHSRLGHNLVSWRLQWKNGKLTACSSSSNAMLGEVFQYPSKIEVVLDCEGGTLSFHTIKSRREHLYTFKTVFKEPVYPAFSIHSNTSESWITLHSQM

Target ORF information:

RefSeq Version	XM_014972407.1
Organism	Poecilia mexicana
Definition	Poecilia mexicana E3 ubiquitin/ISG15 ligase TRIM25-like (LOC106906933), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014972407.1

ORF Insert Sequence:

ATGAAAGTTT TGTCAAGAAG AGAGACAAGA ATAAAGAAAG AAACTACAAC CAGAAGAGAA 
AAAAAACTGC AATTCTTTTC AACTTTCAGT ATGTTTTTAT CAAAGCAAGA GGACCAGCTT 
GCCCAGGAGC TAAGCTGTCC CATCTGTCTT CAGCTCTACA ACGATCCAGT TGTTCTACCC 
TGTGGGCACA ACTACTGTCG AGCTTGCATC TCTAAGTCTG CTGACTCCAT AGACAGTGGC 
AAAGAACCTC TACGCTGCCC AGAGTGTCGC GAGGCCTATC ATGGGGTCGA TACTCTGCAG 
AAAAATTTCA AACTTTCCAA CATTGTCGAG GGTTTCCAAG CTTCTTCCCA AAACATTTAC 
GAAGAGATCC AGGATGTGAT CAAACCAACG AACCCAGACG CGGATGATGT CATTTTCTGT 
GACCAATGCA TAGATGACAA GACTCCGGCA GTGAAGACCT GTCTGAAGTG TGAGGTGTCG 
CTGTGCTTCA GGCATCTCCA GAAGCATAAC GAGAGGGAGT CCTTCAAGAC ACACTCTCTG 
GTGGAACCTC TGAAGGATTT GGGGAGCAGG GCCTGCCCCG TCCATAACCT TCCACTTGAG 
TACTTTTGTT CTAATGACAT GAGGTCTCTG TGCATCACAT GTTTGGAAGG CCATGACCAG 
AACCACGACG TCCTCACCTT CAGTGTGGCA GAGGAGGAGA TGAGACGAGC CTTGGAGAGC 
CGTAGCAAGG TGGTTTCCAG CCGACTGCAA CTGACAGAGT GCCTCCTTCA GAAGACACAA 
GAGGACCAGG GAGCCTCCGA GGCTGTTGGA GATAAAATGG TCAACAAGGC AGTCTCACTG 
ATGGATAGTA TGGCTGCAAT AGTGGACAGG TTCAGGGAGC GATTGCGTTT GCTTTTGGAG 
GAGGAGAGAG GGCAGCGGCG CAAAAGCTGG CAGCTTGGAG TGAGTGCACT GGAGGAGCAG 
CAGCAGCAGC TGCAGGAGGC CCAGAAAAGT GCATCCGAGG CGCTCAGCGA GACAGACACA 
TGCGCCTTCA TACACAGATT CATGATGATT GAACAGAAAC TGAGAGATGC TGCCACAGGC 
AGTCTTCCCA GCACGATTCC CTCAAAGGTC GACCTGAACA CCAAGCGACT CCAGACAGAC 
CTCAAAACCA ACGACTTCCG CACAGAAATA GTCCGTCTCC TTGACACCCT TCACATCCTG 
CTCAATCCTC TGGAGGTCAC CTTCAACGTC TGTACTGCCC ACCCCAGCTT GTTGGTGTCC 
AACGACCTGC ACACAGTCAG ATGCACCAGC TCAAAGCAGT CCTACGTAGA GCACCCGGAG 
CGCTTCCTTA GTGCACCTCA GATCCTGTGC AGCCAGGGCT TTTCCAGAGG AACACATATC 
TGGGTGGCAG AAATGGGCAG CAACTGCATG TGGTCCCTTG GCGCATGCTA CAAGAGCATC 
CCTCGCCGTG GAGACCACAG CCGCCTGGGG CACAACTTGG TGTCCTGGAG ACTACAGTGG 
AAAAATGGCA AGCTAACTGC GTGCTCTTCA TCTAGCAATG CTATGCTGGG GGAGGTGTTC 
CAATATCCAT CTAAGATTGA GGTTGTGCTT GACTGTGAAG GTGGAACCCT GTCCTTCCAC 
ACCATCAAAT CGCGCAGAGA ACACCTTTAC ACCTTTAAGA CTGTGTTTAA GGAACCTGTT 
TACCCAGCTT TTAGCATACA TTCAAATACA TCAGAGTCTT GGATCACTCT ACATAGTCAG 
ATGTGA