LOC103146249 cDNA ORF clone, Poecilia formosa(Amazon molly)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC103146249
Entrez Gene ID	103146249
Full Name	uncharacterized LOC103146249
Gene Type	protein-coding
Organism	Poecilia formosa(Amazon molly)

mRNA and Protein(s)

mRNA	Protein	Name
XM_007564088.2	XP_007564150.1	uncharacterized protein LOC103146249 isoform X1
XM_016677370.1	XP_016532856.1	uncharacterized protein LOC103146249 isoform X2

Pathways from BioSystems
Gene Ontology

The following LOC103146249 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC103146249 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPo146684	XM_007564088.2 Latest version!	Poecilia formosa uncharacterized LOC103146249 (LOC103146249), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OPo146685	XM_016677370.1 Latest version!	Poecilia formosa uncharacterized LOC103146249 (LOC103146249), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC103146249 ( XM_007564088.2 ) cDNA ORF clone, Poecilia formosa(Amazon molly) -> XP_007564150.1 Poecilia formosa uncharacterized LOC103146249 (LOC103146249), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPo146684
Clone ID Related Accession (Same CDS sequence)	XM_007564088.2
Accession Version	XM_007564088.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3606bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-05-02
Organism	Poecilia formosa(Amazon molly)
Product	uncharacterized protein LOC103146249 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006800093.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 3, 2016 this sequence version replaced XM_007564088.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia formosa Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTCGAT TCCTTTGGAC GATGGCTCTC TTCTTCTGGT ATTATTTGCT ACTTCACAGC 
CGAGCTCTGG CAGACGAAGA AGCCACTGGG GAACTGACAC CATCAAGCAA AAATTCTCCT 
GAAGAGAAGT CTAGCATTTA CTCATGGCTT CCATCCATGC CCAAGTTTCC CTCATTTGGC 
TTATTTGGGA GATCAGGTGG CAAAAATGAA GACGCTGTCT CAACTACAAC TGCCAAAGGG 
GTGACAGAAA TAATCGGTCT CTTTGAAGAC CACACGGGAT CAGGAGAAGG GAGCAATTCT 
GAGGAATCTC AACAAACCAC AACGTTGACT CAAGGACTTT TTATGAGTGT GGCAAAGATA 
AGAACTGAGC CACCTCCCAC TGGGACATCA GATTTGCCAT ACAGCAGCAT AGCTCAGAGC 
AACAGTGAGA CTTTTGTTTC TCCCACAAGC AGCTACATCA GAAATACTCT GTTCACAAAC 
AGTCCTCACA GAAGTAACAC TCCTGAACAA AACACAACAC ATATGTTAAC ACCATGGAGA 
TCCACGACAG CTGCGACTTC TAGCACAGGT GCCACTGAAA ATCCCCTCCC CAATAGTGTT 
TACCGGGAGA CCATCTTTCA TACAGAAAGG ATTTCTTCAA CAATCCCACC AGAAGCTACA 
GCTCCCACTG CCCTGACATG GAAAACAGTC CGAACCACAA CACCCAAACC GGGACTGGTG 
GAGACTACTC AAACCAGCAC ACACCTTCTG GGGCTTGTTA CTCTTCCAGC CATTTCAGAA 
AGTAATTTTG TTGGGGAAGA AGAAGATTTA TCTGTTAGTG TTGCCCTTCA TTCAGTTAAA 
GGAACATCAG AAATTCACTC CACACAAAGT AACTGGAACT TGGGCTTGTC TGAGATCAGT 
CTGGTGAACA CCGAGCAGCA GCCAGGGGTT ACTTCGACTT TTCTAAGGAC AGATCCTACC 
ACAGTAGAAG GCACAACCTC AGACATTAAC CCCACACAAA ACCCTTTAGA CCTCTCTGAG 
ACCAGTACGG TGAACTTCGA ACAACATTCA GAAGTATTCC CTGCCGCCCT CAAGACGGAT 
CATAACTTAG AAGTGGTCAG GACAACATCA GAAATATATC ACACACAAAG CTACTTATAC 
TTGGGCTTCT CTGAGACCAC TACGACGAAT GCCAAACAAG AGCCAGGAGT TTTCTCTTCT 
ACCCTCAGGA AGGATTCAAA TTTAGTGGCG GCGACCACAA AAGCAGAAAT CCAGACTACA 
CAAAGCCATT TGGACTTGGG CTTTTCTAAG ACGAGTACAG TAAATGCTGA ACTACAGCCT 
CGGGTATTCT CTACCACCCT CTGGATCGAT CGTAATTTAG CTGTGGAATC CATACCAAGC 
ACAACTCAAA GCCAAAGGGC CAACTTTCCA ATTGTAGAAG AGCTACCAGG AAAAGAGACT 
AATGAAGAGG ATGAGGCTGT GGTTACTTCA ACTACAGACG GGGACGCTGA CACACATTTG 
ACAGACACTT CCACATCATC TGCCCAGCTA CTGAAAACAT CCACGGCACA CAACAAAGGT 
ATCCTCTCTG AATCTCCATC ATTACTTCTG TACACTGAAG ACTGGACATC TAACCTACCA 
AGCAGTGACA GCACCTTACT TCCAGACTGC AATAAGGAAC GCTCTGGAAT CTGCAACCTC 
TCCTACACCT GGGATGTCAC TACTTCCCTG AGGATGAAAC CCACACAAAA TATCACTACC 
ACAAACCAGT CAACCAACGC CTTCCTAATC CCAGCTACAC CCATGTTGGT GCCCTTATAC 
ACTGACTGGA ACAGCGCCAT GGCGGCCTGG GGCTTGGCGT GGGAGGCCCA TGTCTACGGT 
GCAGGCGGAG TCTTTGCGGC ACTAACACTC GCCTCTGCAC TCAATCTGCT TTGCTTGCCA 
CTGAGATGTC CATCTGGGTG TGGCTATTTT GCTCTAGTCA GCCTGTTCCT TCTAGCTGCT 
GCATGCACCA GGTCCTTCTC CCTTCTTTAT GATGCCTATG GCCACCAGGA TCGCATATCC 
TCCACAGAGG CCTCACTCAT GCTCTTTGAA GCGCCTTTTC CCTGTCTTAC TGCGGCTTTC 
GGTTTGGTTT TTCTTCTCCT CTCAATGCGC TCAAGAATGC AGCTCTCATA TTCAGCCTTT 
CAGAGACCCT GTTTCCTGGC CTGCCTCGTT GTCATGCACT TTGCTGCTGC ATTTGGTCCA 
GTGACTTTCT TGAAGTTCTA CCGCCAAACG CCCCCTTTGT GCCTCTTTCT GTCGTTGATC 
TCTCGTGGAG CCTTTGTGGC GCTCGCAACA TTTCTATCTG CTGCCTATTT TGTTTTCTAT 
ATCTATGTGC GGGCAGACTC AAAGCACATC TATCATCTAA ACAACACGTC TCCAACACCA 
GCTGAACGCT ACAACCGTTG CCCATTTGCT GAGAGTCGGG ACTGGAACCG TGCAGCTATA 
ACCGTTTGTC TTTCAGCATT GTTTTCTCTA GCTTGTGCAG GACTGCAGTT TTATGCAATG 
CTTAACGCTA TGGGTGTTAT TGGTGGAGAG GAGGTCTTCT ACCCTTGGCC CTGGTGGGCT 
TTCCAGTTTA GCTGTAGACT GTGTGAACTC GGGGTCTGTC TCACCTTAGC TTTAGTGGTT 
GCTCAACCAG TTTTTTGTTC TGACCAGCTT CCAGCAGCTG GAAGTTGTTG GACTGAACTC 
CTAGCATCCA AATCCCCCAT CCTGCCTGGG ACCTACCAGT GGACACTCAG CCAGCAGGAG 
AAGCTTGCTA TTGTTGACAC TGTTGGACTT GGCGAGACAG AAAGCCTTCC ACTTTACACT 
CTGATGGATG AGAGGCTTGG AAGCAGCCTG AATGGCTTGG ACCTCCTCTA CCACAGCAAC 
CGGGCCTTAG TATATCGAGA TCTCGACTTG GACTTAGGGT TATTGGGTTC AGAAAAACCT 
GAGGACGGGG GAGGCAGAGA GCCCTCAGGT GGATCTTCAT TTTCAAGCGA CTCCACTGCA 
GACCTGCGAC CTCCTTCACC AATCAACTTG CGCCGTAGCA TAGACGAAGC ACTCTTCAGT 
GAAGCATTGT TTCCCATGAG CCTCTTCAGT CCTGTTAGAC CCATTCGCTG CAGTGACGCA 
TCTGTCAACA ACCACTGGAC GCTTCCAAGC AATGGTCCCT GTGAGCCTCT CTCCACCGAC 
TCCAGTCTTT ATCGAACCTC ATCCTGCCTT GAAATGGCCT CTCAGCTCCA GTTTTCCGCC 
AGCCAGTCTC AAGGTGCAGC CACAGAAGGA GCTCCAACAT CTCCCTCCAC GGGCTCATCC 
TCCAGCAATT CGTCAGCTGA ACGTTGGAGA AGCAGTTCCT CCTCCTGCTT TCTGTACCGT 
GCCTCTCTTG CTGGCTCCTC TCTTGTTCTT TGTCCAAGTT CAGAAAGACA CACAAGGGAG 
CTTCCACAGG AAGGCTCAAA TGTGGCCTCC CATAACCCAC AAAGACACTA CCAAGCTCTG 
GGAGCTGCCT CACAGGAAAG CCTGAACCTG TCAACTGAGA CAGATCGATC TGTGCAGCAG 
GAATTCATTA CGGTTTGCAG ACAAATGGAT TCGTTAAGCA TCTGCAGTGA GACTATAGAT 
TTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007564150.1
CDS	196..3801
Translation	MLRFLWTMALFFWYYLLLHSRALADEEATGELTPSSKNSPEEKSSIYSWLPSMPKFPSFGLFGRSGGKNEDAVSTTTAKGVTEIIGLFEDHTGSGEGSNSEESQQTTTLTQGLFMSVAKIRTEPPPTGTSDLPYSSIAQSNSETFVSPTSSYIRNTLFTNSPHRSNTPEQNTTHMLTPWRSTTAATSSTGATENPLPNSVYRETIFHTERISSTIPPEATAPTALTWKTVRTTTPKPGLVETTQTSTHLLGLVTLPAISESNFVGEEEDLSVSVALHSVKGTSEIHSTQSNWNLGLSEISLVNTEQQPGVTSTFLRTDPTTVEGTTSDINPTQNPLDLSETSTVNFEQHSEVFPAALKTDHNLEVVRTTSEIYHTQSYLYLGFSETTTTNAKQEPGVFSSTLRKDSNLVAATTKAEIQTTQSHLDLGFSKTSTVNAELQPRVFSTTLWIDRNLAVESIPSTTQSQRANFPIVEELPGKETNEEDEAVVTSTTDGDADTHLTDTSTSSAQLLKTSTAHNKGILSESPSLLLYTEDWTSNLPSSDSTLLPDCNKERSGICNLSYTWDVTTSLRMKPTQNITTTNQSTNAFLIPATPMLVPLYTDWNSAMAAWGLAWEAHVYGAGGVFAALTLASALNLLCLPLRCPSGCGYFALVSLFLLAAACTRSFSLLYDAYGHQDRISSTEASLMLFEAPFPCLTAAFGLVFLLLSMRSRMQLSYSAFQRPCFLACLVVMHFAAAFGPVTFLKFYRQTPPLCLFLSLISRGAFVALATFLSAAYFVFYIYVRADSKHIYHLNNTSPTPAERYNRCPFAESRDWNRAAITVCLSALFSLACAGLQFYAMLNAMGVIGGEEVFYPWPWWAFQFSCRLCELGVCLTLALVVAQPVFCSDQLPAAGSCWTELLASKSPILPGTYQWTLSQQEKLAIVDTVGLGETESLPLYTLMDERLGSSLNGLDLLYHSNRALVYRDLDLDLGLLGSEKPEDGGGREPSGGSSFSSDSTADLRPPSPINLRRSIDEALFSEALFPMSLFSPVRPIRCSDASVNNHWTLPSNGPCEPLSTDSSLYRTSSCLEMASQLQFSASQSQGAATEGAPTSPSTGSSSSNSSAERWRSSSSSCFLYRASLAGSSLVLCPSSERHTRELPQEGSNVASHNPQRHYQALGAASQESLNLSTETDRSVQQEFITVCRQMDSLSICSETIDL

Target ORF information:

RefSeq Version	XM_007564088.2
Organism	Poecilia formosa(Amazon molly)
Definition	Poecilia formosa uncharacterized LOC103146249 (LOC103146249), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007564088.2

ORF Insert Sequence:

ATGCTTCGAT TCCTTTGGAC GATGGCTCTC TTCTTCTGGT ATTATTTGCT ACTTCACAGC 
CGAGCTCTGG CAGACGAAGA AGCCACTGGG GAACTGACAC CATCAAGCAA AAATTCTCCT 
GAAGAGAAGT CTAGCATTTA CTCATGGCTT CCATCCATGC CCAAGTTTCC CTCATTTGGC 
TTATTTGGGA GATCAGGTGG CAAAAATGAA GACGCTGTCT CAACTACAAC TGCCAAAGGG 
GTGACAGAAA TAATCGGTCT CTTTGAAGAC CACACGGGAT CAGGAGAAGG GAGCAATTCT 
GAGGAATCTC AACAAACCAC AACGTTGACT CAAGGACTTT TTATGAGTGT GGCAAAGATA 
AGAACTGAGC CACCTCCCAC TGGGACATCA GATTTGCCAT ACAGCAGCAT AGCTCAGAGC 
AACAGTGAGA CTTTTGTTTC TCCCACAAGC AGCTACATCA GAAATACTCT GTTCACAAAC 
AGTCCTCACA GAAGTAACAC TCCTGAACAA AACACAACAC ATATGTTAAC ACCATGGAGA 
TCCACGACAG CTGCGACTTC TAGCACAGGT GCCACTGAAA ATCCCCTCCC CAATAGTGTT 
TACCGGGAGA CCATCTTTCA TACAGAAAGG ATTTCTTCAA CAATCCCACC AGAAGCTACA 
GCTCCCACTG CCCTGACATG GAAAACAGTC CGAACCACAA CACCCAAACC GGGACTGGTG 
GAGACTACTC AAACCAGCAC ACACCTTCTG GGGCTTGTTA CTCTTCCAGC CATTTCAGAA 
AGTAATTTTG TTGGGGAAGA AGAAGATTTA TCTGTTAGTG TTGCCCTTCA TTCAGTTAAA 
GGAACATCAG AAATTCACTC CACACAAAGT AACTGGAACT TGGGCTTGTC TGAGATCAGT 
CTGGTGAACA CCGAGCAGCA GCCAGGGGTT ACTTCGACTT TTCTAAGGAC AGATCCTACC 
ACAGTAGAAG GCACAACCTC AGACATTAAC CCCACACAAA ACCCTTTAGA CCTCTCTGAG 
ACCAGTACGG TGAACTTCGA ACAACATTCA GAAGTATTCC CTGCCGCCCT CAAGACGGAT 
CATAACTTAG AAGTGGTCAG GACAACATCA GAAATATATC ACACACAAAG CTACTTATAC 
TTGGGCTTCT CTGAGACCAC TACGACGAAT GCCAAACAAG AGCCAGGAGT TTTCTCTTCT 
ACCCTCAGGA AGGATTCAAA TTTAGTGGCG GCGACCACAA AAGCAGAAAT CCAGACTACA 
CAAAGCCATT TGGACTTGGG CTTTTCTAAG ACGAGTACAG TAAATGCTGA ACTACAGCCT 
CGGGTATTCT CTACCACCCT CTGGATCGAT CGTAATTTAG CTGTGGAATC CATACCAAGC 
ACAACTCAAA GCCAAAGGGC CAACTTTCCA ATTGTAGAAG AGCTACCAGG AAAAGAGACT 
AATGAAGAGG ATGAGGCTGT GGTTACTTCA ACTACAGACG GGGACGCTGA CACACATTTG 
ACAGACACTT CCACATCATC TGCCCAGCTA CTGAAAACAT CCACGGCACA CAACAAAGGT 
ATCCTCTCTG AATCTCCATC ATTACTTCTG TACACTGAAG ACTGGACATC TAACCTACCA 
AGCAGTGACA GCACCTTACT TCCAGACTGC AATAAGGAAC GCTCTGGAAT CTGCAACCTC 
TCCTACACCT GGGATGTCAC TACTTCCCTG AGGATGAAAC CCACACAAAA TATCACTACC 
ACAAACCAGT CAACCAACGC CTTCCTAATC CCAGCTACAC CCATGTTGGT GCCCTTATAC 
ACTGACTGGA ACAGCGCCAT GGCGGCCTGG GGCTTGGCGT GGGAGGCCCA TGTCTACGGT 
GCAGGCGGAG TCTTTGCGGC ACTAACACTC GCCTCTGCAC TCAATCTGCT TTGCTTGCCA 
CTGAGATGTC CATCTGGGTG TGGCTATTTT GCTCTAGTCA GCCTGTTCCT TCTAGCTGCT 
GCATGCACCA GGTCCTTCTC CCTTCTTTAT GATGCCTATG GCCACCAGGA TCGCATATCC 
TCCACAGAGG CCTCACTCAT GCTCTTTGAA GCGCCTTTTC CCTGTCTTAC TGCGGCTTTC 
GGTTTGGTTT TTCTTCTCCT CTCAATGCGC TCAAGAATGC AGCTCTCATA TTCAGCCTTT 
CAGAGACCCT GTTTCCTGGC CTGCCTCGTT GTCATGCACT TTGCTGCTGC ATTTGGTCCA 
GTGACTTTCT TGAAGTTCTA CCGCCAAACG CCCCCTTTGT GCCTCTTTCT GTCGTTGATC 
TCTCGTGGAG CCTTTGTGGC GCTCGCAACA TTTCTATCTG CTGCCTATTT TGTTTTCTAT 
ATCTATGTGC GGGCAGACTC AAAGCACATC TATCATCTAA ACAACACGTC TCCAACACCA 
GCTGAACGCT ACAACCGTTG CCCATTTGCT GAGAGTCGGG ACTGGAACCG TGCAGCTATA 
ACCGTTTGTC TTTCAGCATT GTTTTCTCTA GCTTGTGCAG GACTGCAGTT TTATGCAATG 
CTTAACGCTA TGGGTGTTAT TGGTGGAGAG GAGGTCTTCT ACCCTTGGCC CTGGTGGGCT 
TTCCAGTTTA GCTGTAGACT GTGTGAACTC GGGGTCTGTC TCACCTTAGC TTTAGTGGTT 
GCTCAACCAG TTTTTTGTTC TGACCAGCTT CCAGCAGCTG GAAGTTGTTG GACTGAACTC 
CTAGCATCCA AATCCCCCAT CCTGCCTGGG ACCTACCAGT GGACACTCAG CCAGCAGGAG 
AAGCTTGCTA TTGTTGACAC TGTTGGACTT GGCGAGACAG AAAGCCTTCC ACTTTACACT 
CTGATGGATG AGAGGCTTGG AAGCAGCCTG AATGGCTTGG ACCTCCTCTA CCACAGCAAC 
CGGGCCTTAG TATATCGAGA TCTCGACTTG GACTTAGGGT TATTGGGTTC AGAAAAACCT 
GAGGACGGGG GAGGCAGAGA GCCCTCAGGT GGATCTTCAT TTTCAAGCGA CTCCACTGCA 
GACCTGCGAC CTCCTTCACC AATCAACTTG CGCCGTAGCA TAGACGAAGC ACTCTTCAGT 
GAAGCATTGT TTCCCATGAG CCTCTTCAGT CCTGTTAGAC CCATTCGCTG CAGTGACGCA 
TCTGTCAACA ACCACTGGAC GCTTCCAAGC AATGGTCCCT GTGAGCCTCT CTCCACCGAC 
TCCAGTCTTT ATCGAACCTC ATCCTGCCTT GAAATGGCCT CTCAGCTCCA GTTTTCCGCC 
AGCCAGTCTC AAGGTGCAGC CACAGAAGGA GCTCCAACAT CTCCCTCCAC GGGCTCATCC 
TCCAGCAATT CGTCAGCTGA ACGTTGGAGA AGCAGTTCCT CCTCCTGCTT TCTGTACCGT 
GCCTCTCTTG CTGGCTCCTC TCTTGTTCTT TGTCCAAGTT CAGAAAGACA CACAAGGGAG 
CTTCCACAGG AAGGCTCAAA TGTGGCCTCC CATAACCCAC AAAGACACTA CCAAGCTCTG 
GGAGCTGCCT CACAGGAAAG CCTGAACCTG TCAACTGAGA CAGATCGATC TGTGCAGCAG 
GAATTCATTA CGGTTTGCAG ACAAATGGAT TCGTTAAGCA TCTGCAGTGA GACTATAGAT 
TTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC103146249 ( XM_016677370.1 ) cDNA ORF clone, Poecilia formosa(Amazon molly) -> XP_016532856.1 Poecilia formosa uncharacterized LOC103146249 (LOC103146249), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPo146685
Clone ID Related Accession (Same CDS sequence)	XM_016677370.1
Accession Version	XM_016677370.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3468bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-05-02
Organism	Poecilia formosa(Amazon molly)
Product	uncharacterized protein LOC103146249 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006800093.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia formosa Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTCGAT TCCTTTGGAC GATGGCTCTC TTCTTCTGGT ATTATTTGCT ACTTCACAGC 
CGAGCTCTGG CAGACGAAGA AGCCACTGGG GAACTGACAC CATCAAGCAA AAATTCTCCT 
GAAGAGAAGT CTAGCATTTA CTCATGGCTT CCATCCATGC CCAAGTTTCC CTCATTTGGC 
TTATTTGGGA GATCAGGTGG CAAAAATGAA GACGCTGTCT CAACTACAAC TGCCAAAGGG 
GTGACAGAAA TAATCGGTCT CTTTGAAGAC CACACGGGAT CAGGAGAAGG GAGCAATTCT 
GAGGAATCTC AACAAACCAC AACGTTGACT CAAGGACTTT TTATGAGTGT GGCAAAGATA 
AGAACTGAGC CACCTCCCAC TGGGACATCA GATTTGCCAT ACAGCAGCAT AGCTCAGAGC 
AACAGTGAGA CTTTTGTTTC TCCCACAAGC AGCTACATCA GAAATACTCT GTTCACAAAC 
AGTCCTCACA GAAGTAACAC TCCTGAACAA AACACAACAC ATATGTTAAC ACCATGGAGA 
TCCACGACAG CTGCGACTTC TAGCACAGGT GCCACTGAAA ATCCCCTCCC CAATAGTGTT 
TACCGGGAGA CCATCTTTCA TACAGAAAGG ATTTCTTCAA CAATCCCACC AGAAGCTACA 
GCTCCCACTG CCCTGACATG GAAAACAGTC CGAACCACAA CACCCAAACC GGGACTGGTG 
GAGACTACTC AAACCAGCAC ACACCTTCTG GGGCTTGTTA CTCTTCCAGC CATTTCAGAA 
AGTAATTTTG TTGGGGAAGA AGAAGATTTA TCTGTTAGTG TTGCCCTTCA TTCAGTTAAA 
GGAACATCAG AAATTCACTC CACACAAAGT AACTGGAACT TGGGCTTGTC TGAGATCAGT 
CTGGTGAACA CCGAGCAGCA GCCAGGGGTT ACTTCGACTT TTCTAAGGAC AGATCCTACC 
ACAGTAGAAG GCACAACCTC AGACATTAAC CCCACACAAA ACCCTTTAGA CCTCTCTGAG 
ACCAGTACGG TGAACTTCGA ACAACATTCA GAAGTATTCC CTGCCGCCCT CAAGACGGAT 
CATAACTTAG AAGTGGTCAG GACAACATCA GAAATATATC ACACACAAAG CTACTTATAC 
TTGGGCTTCT CTGAGACCAC TACGACGAAT GCCAAACAAG AGCCAGGAGT TTTCTCTTCT 
ACCCTCAGGA AGGATTCAAA TTTAGTGGCG GCGACCACAA AAGCAGAAAT CCAGACTACA 
CAAAGCCATT TGGACTTGGG CTTTTCTAAG ACGAGTACAG TAAATGCTGA ACTACAGCCT 
CGGGTATTCT CTACCACCCT CTGGATCGAT CGTAATTTAG CTGTGGAATC CATACCAAGC 
ACAACTCAAA GCCAAAGGGC CAACTTTCCA ATTGTAGAAG GTATCCTCTC TGAATCTCCA 
TCATTACTTC TGTACACTGA AGACTGGACA TCTAACCTAC CAAGCAGTGA CAGCACCTTA 
CTTCCAGACT GCAATAAGGA ACGCTCTGGA ATCTGCAACC TCTCCTACAC CTGGGATGTC 
ACTACTTCCC TGAGGATGAA ACCCACACAA AATATCACTA CCACAAACCA GTCAACCAAC 
GCCTTCCTAA TCCCAGCTAC ACCCATGTTG GTGCCCTTAT ACACTGACTG GAACAGCGCC 
ATGGCGGCCT GGGGCTTGGC GTGGGAGGCC CATGTCTACG GTGCAGGCGG AGTCTTTGCG 
GCACTAACAC TCGCCTCTGC ACTCAATCTG CTTTGCTTGC CACTGAGATG TCCATCTGGG 
TGTGGCTATT TTGCTCTAGT CAGCCTGTTC CTTCTAGCTG CTGCATGCAC CAGGTCCTTC 
TCCCTTCTTT ATGATGCCTA TGGCCACCAG GATCGCATAT CCTCCACAGA GGCCTCACTC 
ATGCTCTTTG AAGCGCCTTT TCCCTGTCTT ACTGCGGCTT TCGGTTTGGT TTTTCTTCTC 
CTCTCAATGC GCTCAAGAAT GCAGCTCTCA TATTCAGCCT TTCAGAGACC CTGTTTCCTG 
GCCTGCCTCG TTGTCATGCA CTTTGCTGCT GCATTTGGTC CAGTGACTTT CTTGAAGTTC 
TACCGCCAAA CGCCCCCTTT GTGCCTCTTT CTGTCGTTGA TCTCTCGTGG AGCCTTTGTG 
GCGCTCGCAA CATTTCTATC TGCTGCCTAT TTTGTTTTCT ATATCTATGT GCGGGCAGAC 
TCAAAGCACA TCTATCATCT AAACAACACG TCTCCAACAC CAGCTGAACG CTACAACCGT 
TGCCCATTTG CTGAGAGTCG GGACTGGAAC CGTGCAGCTA TAACCGTTTG TCTTTCAGCA 
TTGTTTTCTC TAGCTTGTGC AGGACTGCAG TTTTATGCAA TGCTTAACGC TATGGGTGTT 
ATTGGTGGAG AGGAGGTCTT CTACCCTTGG CCCTGGTGGG CTTTCCAGTT TAGCTGTAGA 
CTGTGTGAAC TCGGGGTCTG TCTCACCTTA GCTTTAGTGG TTGCTCAACC AGTTTTTTGT 
TCTGACCAGC TTCCAGCAGC TGGAAGTTGT TGGACTGAAC TCCTAGCATC CAAATCCCCC 
ATCCTGCCTG GGACCTACCA GTGGACACTC AGCCAGCAGG AGAAGCTTGC TATTGTTGAC 
ACTGTTGGAC TTGGCGAGAC AGAAAGCCTT CCACTTTACA CTCTGATGGA TGAGAGGCTT 
GGAAGCAGCC TGAATGGCTT GGACCTCCTC TACCACAGCA ACCGGGCCTT AGTATATCGA 
GATCTCGACT TGGACTTAGG GTTATTGGGT TCAGAAAAAC CTGAGGACGG GGGAGGCAGA 
GAGCCCTCAG GTGGATCTTC ATTTTCAAGC GACTCCACTG CAGACCTGCG ACCTCCTTCA 
CCAATCAACT TGCGCCGTAG CATAGACGAA GCACTCTTCA GTGAAGCATT GTTTCCCATG 
AGCCTCTTCA GTCCTGTTAG ACCCATTCGC TGCAGTGACG CATCTGTCAA CAACCACTGG 
ACGCTTCCAA GCAATGGTCC CTGTGAGCCT CTCTCCACCG ACTCCAGTCT TTATCGAACC 
TCATCCTGCC TTGAAATGGC CTCTCAGCTC CAGTTTTCCG CCAGCCAGTC TCAAGGTGCA 
GCCACAGAAG GAGCTCCAAC ATCTCCCTCC ACGGGCTCAT CCTCCAGCAA TTCGTCAGCT 
GAACGTTGGA GAAGCAGTTC CTCCTCCTGC TTTCTGTACC GTGCCTCTCT TGCTGGCTCC 
TCTCTTGTTC TTTGTCCAAG TTCAGAAAGA CACACAAGGG AGCTTCCACA GGAAGGCTCA 
AATGTGGCCT CCCATAACCC ACAAAGACAC TACCAAGCTC TGGGAGCTGC CTCACAGGAA 
AGCCTGAACC TGTCAACTGA GACAGATCGA TCTGTGCAGC AGGAATTCAT TACGGTTTGC 
AGACAAATGG ATTCGTTAAG CATCTGCAGT GAGACTATAG ATTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016532856.1
CDS	196..3663
Translation	MLRFLWTMALFFWYYLLLHSRALADEEATGELTPSSKNSPEEKSSIYSWLPSMPKFPSFGLFGRSGGKNEDAVSTTTAKGVTEIIGLFEDHTGSGEGSNSEESQQTTTLTQGLFMSVAKIRTEPPPTGTSDLPYSSIAQSNSETFVSPTSSYIRNTLFTNSPHRSNTPEQNTTHMLTPWRSTTAATSSTGATENPLPNSVYRETIFHTERISSTIPPEATAPTALTWKTVRTTTPKPGLVETTQTSTHLLGLVTLPAISESNFVGEEEDLSVSVALHSVKGTSEIHSTQSNWNLGLSEISLVNTEQQPGVTSTFLRTDPTTVEGTTSDINPTQNPLDLSETSTVNFEQHSEVFPAALKTDHNLEVVRTTSEIYHTQSYLYLGFSETTTTNAKQEPGVFSSTLRKDSNLVAATTKAEIQTTQSHLDLGFSKTSTVNAELQPRVFSTTLWIDRNLAVESIPSTTQSQRANFPIVEGILSESPSLLLYTEDWTSNLPSSDSTLLPDCNKERSGICNLSYTWDVTTSLRMKPTQNITTTNQSTNAFLIPATPMLVPLYTDWNSAMAAWGLAWEAHVYGAGGVFAALTLASALNLLCLPLRCPSGCGYFALVSLFLLAAACTRSFSLLYDAYGHQDRISSTEASLMLFEAPFPCLTAAFGLVFLLLSMRSRMQLSYSAFQRPCFLACLVVMHFAAAFGPVTFLKFYRQTPPLCLFLSLISRGAFVALATFLSAAYFVFYIYVRADSKHIYHLNNTSPTPAERYNRCPFAESRDWNRAAITVCLSALFSLACAGLQFYAMLNAMGVIGGEEVFYPWPWWAFQFSCRLCELGVCLTLALVVAQPVFCSDQLPAAGSCWTELLASKSPILPGTYQWTLSQQEKLAIVDTVGLGETESLPLYTLMDERLGSSLNGLDLLYHSNRALVYRDLDLDLGLLGSEKPEDGGGREPSGGSSFSSDSTADLRPPSPINLRRSIDEALFSEALFPMSLFSPVRPIRCSDASVNNHWTLPSNGPCEPLSTDSSLYRTSSCLEMASQLQFSASQSQGAATEGAPTSPSTGSSSSNSSAERWRSSSSSCFLYRASLAGSSLVLCPSSERHTRELPQEGSNVASHNPQRHYQALGAASQESLNLSTETDRSVQQEFITVCRQMDSLSICSETIDL

Target ORF information:

RefSeq Version	XM_016677370.1
Organism	Poecilia formosa(Amazon molly)
Definition	Poecilia formosa uncharacterized LOC103146249 (LOC103146249), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016677370.1

ORF Insert Sequence:

ATGCTTCGAT TCCTTTGGAC GATGGCTCTC TTCTTCTGGT ATTATTTGCT ACTTCACAGC 
CGAGCTCTGG CAGACGAAGA AGCCACTGGG GAACTGACAC CATCAAGCAA AAATTCTCCT 
GAAGAGAAGT CTAGCATTTA CTCATGGCTT CCATCCATGC CCAAGTTTCC CTCATTTGGC 
TTATTTGGGA GATCAGGTGG CAAAAATGAA GACGCTGTCT CAACTACAAC TGCCAAAGGG 
GTGACAGAAA TAATCGGTCT CTTTGAAGAC CACACGGGAT CAGGAGAAGG GAGCAATTCT 
GAGGAATCTC AACAAACCAC AACGTTGACT CAAGGACTTT TTATGAGTGT GGCAAAGATA 
AGAACTGAGC CACCTCCCAC TGGGACATCA GATTTGCCAT ACAGCAGCAT AGCTCAGAGC 
AACAGTGAGA CTTTTGTTTC TCCCACAAGC AGCTACATCA GAAATACTCT GTTCACAAAC 
AGTCCTCACA GAAGTAACAC TCCTGAACAA AACACAACAC ATATGTTAAC ACCATGGAGA 
TCCACGACAG CTGCGACTTC TAGCACAGGT GCCACTGAAA ATCCCCTCCC CAATAGTGTT 
TACCGGGAGA CCATCTTTCA TACAGAAAGG ATTTCTTCAA CAATCCCACC AGAAGCTACA 
GCTCCCACTG CCCTGACATG GAAAACAGTC CGAACCACAA CACCCAAACC GGGACTGGTG 
GAGACTACTC AAACCAGCAC ACACCTTCTG GGGCTTGTTA CTCTTCCAGC CATTTCAGAA 
AGTAATTTTG TTGGGGAAGA AGAAGATTTA TCTGTTAGTG TTGCCCTTCA TTCAGTTAAA 
GGAACATCAG AAATTCACTC CACACAAAGT AACTGGAACT TGGGCTTGTC TGAGATCAGT 
CTGGTGAACA CCGAGCAGCA GCCAGGGGTT ACTTCGACTT TTCTAAGGAC AGATCCTACC 
ACAGTAGAAG GCACAACCTC AGACATTAAC CCCACACAAA ACCCTTTAGA CCTCTCTGAG 
ACCAGTACGG TGAACTTCGA ACAACATTCA GAAGTATTCC CTGCCGCCCT CAAGACGGAT 
CATAACTTAG AAGTGGTCAG GACAACATCA GAAATATATC ACACACAAAG CTACTTATAC 
TTGGGCTTCT CTGAGACCAC TACGACGAAT GCCAAACAAG AGCCAGGAGT TTTCTCTTCT 
ACCCTCAGGA AGGATTCAAA TTTAGTGGCG GCGACCACAA AAGCAGAAAT CCAGACTACA 
CAAAGCCATT TGGACTTGGG CTTTTCTAAG ACGAGTACAG TAAATGCTGA ACTACAGCCT 
CGGGTATTCT CTACCACCCT CTGGATCGAT CGTAATTTAG CTGTGGAATC CATACCAAGC 
ACAACTCAAA GCCAAAGGGC CAACTTTCCA ATTGTAGAAG GTATCCTCTC TGAATCTCCA 
TCATTACTTC TGTACACTGA AGACTGGACA TCTAACCTAC CAAGCAGTGA CAGCACCTTA 
CTTCCAGACT GCAATAAGGA ACGCTCTGGA ATCTGCAACC TCTCCTACAC CTGGGATGTC 
ACTACTTCCC TGAGGATGAA ACCCACACAA AATATCACTA CCACAAACCA GTCAACCAAC 
GCCTTCCTAA TCCCAGCTAC ACCCATGTTG GTGCCCTTAT ACACTGACTG GAACAGCGCC 
ATGGCGGCCT GGGGCTTGGC GTGGGAGGCC CATGTCTACG GTGCAGGCGG AGTCTTTGCG 
GCACTAACAC TCGCCTCTGC ACTCAATCTG CTTTGCTTGC CACTGAGATG TCCATCTGGG 
TGTGGCTATT TTGCTCTAGT CAGCCTGTTC CTTCTAGCTG CTGCATGCAC CAGGTCCTTC 
TCCCTTCTTT ATGATGCCTA TGGCCACCAG GATCGCATAT CCTCCACAGA GGCCTCACTC 
ATGCTCTTTG AAGCGCCTTT TCCCTGTCTT ACTGCGGCTT TCGGTTTGGT TTTTCTTCTC 
CTCTCAATGC GCTCAAGAAT GCAGCTCTCA TATTCAGCCT TTCAGAGACC CTGTTTCCTG 
GCCTGCCTCG TTGTCATGCA CTTTGCTGCT GCATTTGGTC CAGTGACTTT CTTGAAGTTC 
TACCGCCAAA CGCCCCCTTT GTGCCTCTTT CTGTCGTTGA TCTCTCGTGG AGCCTTTGTG 
GCGCTCGCAA CATTTCTATC TGCTGCCTAT TTTGTTTTCT ATATCTATGT GCGGGCAGAC 
TCAAAGCACA TCTATCATCT AAACAACACG TCTCCAACAC CAGCTGAACG CTACAACCGT 
TGCCCATTTG CTGAGAGTCG GGACTGGAAC CGTGCAGCTA TAACCGTTTG TCTTTCAGCA 
TTGTTTTCTC TAGCTTGTGC AGGACTGCAG TTTTATGCAA TGCTTAACGC TATGGGTGTT 
ATTGGTGGAG AGGAGGTCTT CTACCCTTGG CCCTGGTGGG CTTTCCAGTT TAGCTGTAGA 
CTGTGTGAAC TCGGGGTCTG TCTCACCTTA GCTTTAGTGG TTGCTCAACC AGTTTTTTGT 
TCTGACCAGC TTCCAGCAGC TGGAAGTTGT TGGACTGAAC TCCTAGCATC CAAATCCCCC 
ATCCTGCCTG GGACCTACCA GTGGACACTC AGCCAGCAGG AGAAGCTTGC TATTGTTGAC 
ACTGTTGGAC TTGGCGAGAC AGAAAGCCTT CCACTTTACA CTCTGATGGA TGAGAGGCTT 
GGAAGCAGCC TGAATGGCTT GGACCTCCTC TACCACAGCA ACCGGGCCTT AGTATATCGA 
GATCTCGACT TGGACTTAGG GTTATTGGGT TCAGAAAAAC CTGAGGACGG GGGAGGCAGA 
GAGCCCTCAG GTGGATCTTC ATTTTCAAGC GACTCCACTG CAGACCTGCG ACCTCCTTCA 
CCAATCAACT TGCGCCGTAG CATAGACGAA GCACTCTTCA GTGAAGCATT GTTTCCCATG 
AGCCTCTTCA GTCCTGTTAG ACCCATTCGC TGCAGTGACG CATCTGTCAA CAACCACTGG 
ACGCTTCCAA GCAATGGTCC CTGTGAGCCT CTCTCCACCG ACTCCAGTCT TTATCGAACC 
TCATCCTGCC TTGAAATGGC CTCTCAGCTC CAGTTTTCCG CCAGCCAGTC TCAAGGTGCA 
GCCACAGAAG GAGCTCCAAC ATCTCCCTCC ACGGGCTCAT CCTCCAGCAA TTCGTCAGCT 
GAACGTTGGA GAAGCAGTTC CTCCTCCTGC TTTCTGTACC GTGCCTCTCT TGCTGGCTCC 
TCTCTTGTTC TTTGTCCAAG TTCAGAAAGA CACACAAGGG AGCTTCCACA GGAAGGCTCA 
AATGTGGCCT CCCATAACCC ACAAAGACAC TACCAAGCTC TGGGAGCTGC CTCACAGGAA 
AGCCTGAACC TGTCAACTGA GACAGATCGA TCTGTGCAGC AGGAATTCAT TACGGTTTGC 
AGACAAATGG ATTCGTTAAG CATCTGCAGT GAGACTATAG ATTTATAA