nphs1 cDNA ORF clone, Poecilia formosa(Amazon molly)

Gene
Clones
gRNAs

Summary

Gene Symbol	nphs1
Entrez Gene ID	103143095
Full Name	NPHS1, nephrin
Gene Type	protein-coding
Organism	Poecilia formosa(Amazon molly)

mRNA and Protein(s)

mRNA	Protein	Name
XM_016675002.1	XP_016530488.1	nephrin isoform X1
XM_007559501.2	XP_007559563.2	nephrin isoform X2

Pathways from BioSystems
Gene Ontology

The following nphs1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nphs1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPo142677	XM_016675002.1 Latest version!	Poecilia formosa NPHS1 nephrin (nphs1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OPo142678	XM_007559501.2 Latest version!	Poecilia formosa NPHS1 nephrin (nphs1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nphs1 ( XM_016675002.1 ) cDNA ORF clone, Poecilia formosa(Amazon molly) -> XP_016530488.1 Poecilia formosa NPHS1 nephrin (nphs1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPo142677
Clone ID Related Accession (Same CDS sequence)	XM_016675002.1
Accession Version	XM_016675002.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3729bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-05-02
Organism	Poecilia formosa(Amazon molly)
Product	nephrin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006800044.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia formosa Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTTTG TGTCAGCTTG GATCTTGTCT GCTTGGACTC TACCATTTCT GTGTTTCTTT 
TGGGGCACGC ATGCCCAGCA GGCCTTCAGG ACTGAGCCCA GGAACCTCAC TGTGCGAATG 
GGAGCCACAG CTGTGCTGAA GTGTGAGGTC CTCAGGGCCT CGGGGATTGT GCAGTGGGCG 
AAAGACGGCT TTCTTTTGGG ACCTCAGAGA AGCCTGCCAG GCTTCCCACG CTACAGCATG 
ATTGGAAGGA GAGGACAGTA TTATCTCAGG ATTGAAGATG CACAACTTGA TGATGACGCC 
TTCTTTGAAT GCCAAGCCGG CCAATCCGAG AGAAGTGATG CTATTGTCTC CAATCCAGCC 
TTTCTCACCG TGCAAATTCC CCCATCGAAG CCGTATTTTA CCACGGATGT GACAACACCC 
TGGGTGGCGG GAAAGAAGTA CATGGTTGAA TGCGTGGCCC CTGACGCAAA GCCTAGTGCT 
GATATCACTC TGTATAAAGA TGGAGTCGAT CTGACAGGTG CGACGTCTTT TACCACATCT 
GGCTCAAAGG ATAAGCTTCT GAACACGCAC GCCGAAGTAA CGGTCACTGC TCTGAGATCT 
GACAATGGGA GAGAGTTGAC ATGTCACGCC AACAATGAAG CCGTTTCCCA GCCGGTGGTG 
ACCACAGTGA CCATGAATGT GTATTATCCT CCTGAGCCTC CATATATTAT TGGTTTGGAC 
AGAGACGTGG TTAAAGCGGG GAGTTTGCTC GTGTTGGAGT GTGTATCCCA TGGTGGAAAC 
CCCCTGGGCA CTCTTCAATG GACTAAGGAA GGCGAAGTTC TCTCAACAAC CTGGGGAGAG 
GAAGTCGTGG GCCAGAAGTC CAGAAATATC CTCAGACTAC AGATCACCCC TGCTGACAAC 
CAGGCAGAGC TGAGCTGTGA AAGTGCCAAT ATTGTCTCCC CGTCTCCTCT GTCCGTCACC 
CGCAAAATAA CAGTGTTCTT CGGTCCTGCG ACGCTGACGC GGTCGGGTTC GCTGGAGGCC 
ATCGAGGGGC AGGAGTTGAC TCTGAGCTGC CATGCGTCCT CGAGTAATCC CCCCGTCCAC 
ATTCGCTGGT GGCTGGGGCA CAAGGAGCTC AGCAACACCT CTGTCACAAT GGAAGAGGGA 
GAGAACGGAG GGATGACAGC CACCTCCAAT GTGACCCACC GGGTCTCTCG GGAGGAGAAT 
GGACTGAAGC TTACCTGTGA GGCTTTTAAT AACGGCACAC ATCTCTCCAA AATCCAGGCC 
GCTAAGCTCA CTGTGTACTA TCCTCCTCAA AAAGTTTTTA TTGATTCGCC GCCCGAGGAC 
ATCCCTCTGC GCTCGGGCAG CAGAGCCGTG CTTGTCTGCC ACTCGGCTGG AGGGAATCCT 
CCTGCAAAGC TCACCTGGTT TAAGAATGGA AAAAAGGTGT CAGACGCAGA GCCGCAGACG 
TCCTCTAATA TGGTTGTAAC TCGGGTGCTC AACCTACTGC TGACGGCGAG CGACAACCAG 
GCTGCCTACC GCTGTGACGC CACAAATGAG GCGAAGAGAA CGATATCAGC ACATGCTAAG 
CTACTGGTCC AGTTTACAGC CGTGACTATG AAGATTACAG CCAAAGAAGA AGTGTTTTAC 
CGAGGTCAGC AGATGGAACT GGAGTGTCTT TCCGGGAGCA GCAACCCAAA GTCTGACATT 
TCCTGGAAAG TGGGCCCACT GAGATTGCAG GGAGTTGAGG AGGCACCCAA GGAGGCCGAG 
TTTGGGGGCG TTTCTGTGCT CAGTAGGCTT CACCTGAATC TGACTTCACA GCACCACAAC 
CAGAAGGTGA TCTGTCAGGC CTACAGCCCT GTGTTGGAAG AAGGAGCTAG TACGTTTTAC 
CAACTGAATG TGATATATCC TCCTGAATTC AGCTCTGAGC AAGCAACAGA AGTCCAGGTG 
GTGGAGGACG ATGTGGCAAC CATCCCCCTG ATGGTCTCAG CAAACCCCGA GGTGGTCTCC 
TGCATTTGGC TGCGCCGCAA GCAGATGCTG GTCAAAGGGG AAGACATACG CCACCACTGG 
TCAGATGACC ACTCTCTGGA GATTAGGAAT GTGACGAGAA AAGACAAAGG ACTTTACACT 
ATCAAATGTG AAAATGGCGA AGGCGTCAAC GAAACCACTG TTAAGCTGGA TGTTTACTAT 
GCTCCGACTG TCAAGGCGGA GAAAGACCCT GTGTATGTTA ACCAGGGGGG AACAGCAGAC 
CTGATATGTG TGGCAGATGC CAACCCCATT ATACCGGAGA TGTTCTCTTG GAAATGGCTG 
GGAGAGGAAG AAGTGGAGAT GGGAGAGGAG ACCAACGAAA ACAAATCAAG CTTGCTGACC 
ATTCATAATG TGACTCGGGC TCACGCTGGT TTTTACCAGT GTTCGGCCAG CAATGGCATA 
GCACTCCCTG GCAGTGTGGA AATGAGGCTG GTGGTGCAAT TCATTCCGGA GCTTCGGAAA 
GGACCCCAGT GGAGGAAGGT TGCCAGTCGA GGGGACGGCA CTGCCACTGC TGAGCTGGTG 
TGTCAGGCAG AGGGCATTCC TCCTGTAGAG TTTACTTGGG AAAAGAAAGG GATGCTCATA 
GATTTGGCAA ACCCCAGGTA TGAGGAGAGG ACAGCGAGGG ATGGCTCTTT CCACACCAGT 
ACTCTGCGAG TGGTGAATGT GAGCGCCACT CTGGACTACG CCGTCTTCAG CTGCACTGCT 
CGCAACTCAC TTGGGGAAGA CAAGCTAGAC ATCCAGCTCG TCAGCACAAA TCACCCAGAT 
CCACCTTCAT CATTTAGTCT AGTCGGTGTT ACCCATAACT CCGTCACTCT AGAGTGGATC 
CCTGGCTTCA ATGGGGGTTT GCAGCAGAGA TATCGCATAC GATACATCTG GGACCAGTCT 
GTCAGCTACA TGTATGTAGA TGTCGTTCCC CCTGACACAA CAACCTTCAC CGTTACGGGT 
CTGCAGCCGC AGACCACCTA CAACTTCTCA GTTAATGCCC TAAACTCCAT AGGAGAGAGT 
GGCTATGCTG ACGACGGTGC AGTACTGACC ATCACCACTG AGGTGAAGAA AGATATAGAA 
GAGGATCGAT CGGATGATGA CACACAGGCT CCGAGTGGCC TGCCAACCCA TTTGACTGTG 
GCCTTCACAG TGGTGTTCGG AGTCCTGCTG GTGTTGAATT CGCTGGGCTG CTTCTTTGGG 
CGGAAGTGGA AAAACAGGCG TGACAAAACA AAGGCAGAAA GGGACAGTGT GGTGAATGGA 
CAGAAGAATG AAGAGGATGT GTCAAGTCAA TCAACTGGCA TCTCCAATAA GTACGAGAGC 
AGAGAAAAAA TCAACACCGG AGCCCAACGT ACCCTCCTCG TTGACACTGG ATCTGAAACA 
GAGAGCAACG TCTACGAGAG CTACACAGCG TTTCAGGAAG ACTCCCATTA TTATTATCCT 
ACTGGTGACT ATAAACCGTA CCATGGTCCA CATCCTGAGG ACAGACATGG CCTTGGTGCC 
GCTCCTGTGA ACTTTGAAAG CCATTTGTAT GAAGATGTGG GTGATAGACG AGTGTACCAG 
GACACACTGC CTTCCTCTCT TCCTGCCTAC CGTCCACTTT TCTCTGATCG CAGACTGATC 
CAGCAAGCAG AGGACTGGAG GTCACCAACC GACCCTCAGG GTATCAAGAG AGAAGGGCAG 
TTCCCGGATA ATCAACAACC GGAGCGGGAT TATGATCTAC CCTTCGAGTT GCGAGGAGAA 
TTAGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016530488.1
CDS	134..3862
Translation	MEFVSAWILSAWTLPFLCFFWGTHAQQAFRTEPRNLTVRMGATAVLKCEVLRASGIVQWAKDGFLLGPQRSLPGFPRYSMIGRRGQYYLRIEDAQLDDDAFFECQAGQSERSDAIVSNPAFLTVQIPPSKPYFTTDVTTPWVAGKKYMVECVAPDAKPSADITLYKDGVDLTGATSFTTSGSKDKLLNTHAEVTVTALRSDNGRELTCHANNEAVSQPVVTTVTMNVYYPPEPPYIIGLDRDVVKAGSLLVLECVSHGGNPLGTLQWTKEGEVLSTTWGEEVVGQKSRNILRLQITPADNQAELSCESANIVSPSPLSVTRKITVFFGPATLTRSGSLEAIEGQELTLSCHASSSNPPVHIRWWLGHKELSNTSVTMEEGENGGMTATSNVTHRVSREENGLKLTCEAFNNGTHLSKIQAAKLTVYYPPQKVFIDSPPEDIPLRSGSRAVLVCHSAGGNPPAKLTWFKNGKKVSDAEPQTSSNMVVTRVLNLLLTASDNQAAYRCDATNEAKRTISAHAKLLVQFTAVTMKITAKEEVFYRGQQMELECLSGSSNPKSDISWKVGPLRLQGVEEAPKEAEFGGVSVLSRLHLNLTSQHHNQKVICQAYSPVLEEGASTFYQLNVIYPPEFSSEQATEVQVVEDDVATIPLMVSANPEVVSCIWLRRKQMLVKGEDIRHHWSDDHSLEIRNVTRKDKGLYTIKCENGEGVNETTVKLDVYYAPTVKAEKDPVYVNQGGTADLICVADANPIIPEMFSWKWLGEEEVEMGEETNENKSSLLTIHNVTRAHAGFYQCSASNGIALPGSVEMRLVVQFIPELRKGPQWRKVASRGDGTATAELVCQAEGIPPVEFTWEKKGMLIDLANPRYEERTARDGSFHTSTLRVVNVSATLDYAVFSCTARNSLGEDKLDIQLVSTNHPDPPSSFSLVGVTHNSVTLEWIPGFNGGLQQRYRIRYIWDQSVSYMYVDVVPPDTTTFTVTGLQPQTTYNFSVNALNSIGESGYADDGAVLTITTEVKKDIEEDRSDDDTQAPSGLPTHLTVAFTVVFGVLLVLNSLGCFFGRKWKNRRDKTKAERDSVVNGQKNEEDVSSQSTGISNKYESREKINTGAQRTLLVDTGSETESNVYESYTAFQEDSHYYYPTGDYKPYHGPHPEDRHGLGAAPVNFESHLYEDVGDRRVYQDTLPSSLPAYRPLFSDRRLIQQAEDWRSPTDPQGIKREGQFPDNQQPERDYDLPFELRGELV

Target ORF information:

RefSeq Version	XM_016675002.1
Organism	Poecilia formosa(Amazon molly)
Definition	Poecilia formosa NPHS1 nephrin (nphs1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016675002.1

ORF Insert Sequence:

ATGGAGTTTG TGTCAGCTTG GATCTTGTCT GCTTGGACTC TACCATTTCT GTGTTTCTTT 
TGGGGCACGC ATGCCCAGCA GGCCTTCAGG ACTGAGCCCA GGAACCTCAC TGTGCGAATG 
GGAGCCACAG CTGTGCTGAA GTGTGAGGTC CTCAGGGCCT CGGGGATTGT GCAGTGGGCG 
AAAGACGGCT TTCTTTTGGG ACCTCAGAGA AGCCTGCCAG GCTTCCCACG CTACAGCATG 
ATTGGAAGGA GAGGACAGTA TTATCTCAGG ATTGAAGATG CACAACTTGA TGATGACGCC 
TTCTTTGAAT GCCAAGCCGG CCAATCCGAG AGAAGTGATG CTATTGTCTC CAATCCAGCC 
TTTCTCACCG TGCAAATTCC CCCATCGAAG CCGTATTTTA CCACGGATGT GACAACACCC 
TGGGTGGCGG GAAAGAAGTA CATGGTTGAA TGCGTGGCCC CTGACGCAAA GCCTAGTGCT 
GATATCACTC TGTATAAAGA TGGAGTCGAT CTGACAGGTG CGACGTCTTT TACCACATCT 
GGCTCAAAGG ATAAGCTTCT GAACACGCAC GCCGAAGTAA CGGTCACTGC TCTGAGATCT 
GACAATGGGA GAGAGTTGAC ATGTCACGCC AACAATGAAG CCGTTTCCCA GCCGGTGGTG 
ACCACAGTGA CCATGAATGT GTATTATCCT CCTGAGCCTC CATATATTAT TGGTTTGGAC 
AGAGACGTGG TTAAAGCGGG GAGTTTGCTC GTGTTGGAGT GTGTATCCCA TGGTGGAAAC 
CCCCTGGGCA CTCTTCAATG GACTAAGGAA GGCGAAGTTC TCTCAACAAC CTGGGGAGAG 
GAAGTCGTGG GCCAGAAGTC CAGAAATATC CTCAGACTAC AGATCACCCC TGCTGACAAC 
CAGGCAGAGC TGAGCTGTGA AAGTGCCAAT ATTGTCTCCC CGTCTCCTCT GTCCGTCACC 
CGCAAAATAA CAGTGTTCTT CGGTCCTGCG ACGCTGACGC GGTCGGGTTC GCTGGAGGCC 
ATCGAGGGGC AGGAGTTGAC TCTGAGCTGC CATGCGTCCT CGAGTAATCC CCCCGTCCAC 
ATTCGCTGGT GGCTGGGGCA CAAGGAGCTC AGCAACACCT CTGTCACAAT GGAAGAGGGA 
GAGAACGGAG GGATGACAGC CACCTCCAAT GTGACCCACC GGGTCTCTCG GGAGGAGAAT 
GGACTGAAGC TTACCTGTGA GGCTTTTAAT AACGGCACAC ATCTCTCCAA AATCCAGGCC 
GCTAAGCTCA CTGTGTACTA TCCTCCTCAA AAAGTTTTTA TTGATTCGCC GCCCGAGGAC 
ATCCCTCTGC GCTCGGGCAG CAGAGCCGTG CTTGTCTGCC ACTCGGCTGG AGGGAATCCT 
CCTGCAAAGC TCACCTGGTT TAAGAATGGA AAAAAGGTGT CAGACGCAGA GCCGCAGACG 
TCCTCTAATA TGGTTGTAAC TCGGGTGCTC AACCTACTGC TGACGGCGAG CGACAACCAG 
GCTGCCTACC GCTGTGACGC CACAAATGAG GCGAAGAGAA CGATATCAGC ACATGCTAAG 
CTACTGGTCC AGTTTACAGC CGTGACTATG AAGATTACAG CCAAAGAAGA AGTGTTTTAC 
CGAGGTCAGC AGATGGAACT GGAGTGTCTT TCCGGGAGCA GCAACCCAAA GTCTGACATT 
TCCTGGAAAG TGGGCCCACT GAGATTGCAG GGAGTTGAGG AGGCACCCAA GGAGGCCGAG 
TTTGGGGGCG TTTCTGTGCT CAGTAGGCTT CACCTGAATC TGACTTCACA GCACCACAAC 
CAGAAGGTGA TCTGTCAGGC CTACAGCCCT GTGTTGGAAG AAGGAGCTAG TACGTTTTAC 
CAACTGAATG TGATATATCC TCCTGAATTC AGCTCTGAGC AAGCAACAGA AGTCCAGGTG 
GTGGAGGACG ATGTGGCAAC CATCCCCCTG ATGGTCTCAG CAAACCCCGA GGTGGTCTCC 
TGCATTTGGC TGCGCCGCAA GCAGATGCTG GTCAAAGGGG AAGACATACG CCACCACTGG 
TCAGATGACC ACTCTCTGGA GATTAGGAAT GTGACGAGAA AAGACAAAGG ACTTTACACT 
ATCAAATGTG AAAATGGCGA AGGCGTCAAC GAAACCACTG TTAAGCTGGA TGTTTACTAT 
GCTCCGACTG TCAAGGCGGA GAAAGACCCT GTGTATGTTA ACCAGGGGGG AACAGCAGAC 
CTGATATGTG TGGCAGATGC CAACCCCATT ATACCGGAGA TGTTCTCTTG GAAATGGCTG 
GGAGAGGAAG AAGTGGAGAT GGGAGAGGAG ACCAACGAAA ACAAATCAAG CTTGCTGACC 
ATTCATAATG TGACTCGGGC TCACGCTGGT TTTTACCAGT GTTCGGCCAG CAATGGCATA 
GCACTCCCTG GCAGTGTGGA AATGAGGCTG GTGGTGCAAT TCATTCCGGA GCTTCGGAAA 
GGACCCCAGT GGAGGAAGGT TGCCAGTCGA GGGGACGGCA CTGCCACTGC TGAGCTGGTG 
TGTCAGGCAG AGGGCATTCC TCCTGTAGAG TTTACTTGGG AAAAGAAAGG GATGCTCATA 
GATTTGGCAA ACCCCAGGTA TGAGGAGAGG ACAGCGAGGG ATGGCTCTTT CCACACCAGT 
ACTCTGCGAG TGGTGAATGT GAGCGCCACT CTGGACTACG CCGTCTTCAG CTGCACTGCT 
CGCAACTCAC TTGGGGAAGA CAAGCTAGAC ATCCAGCTCG TCAGCACAAA TCACCCAGAT 
CCACCTTCAT CATTTAGTCT AGTCGGTGTT ACCCATAACT CCGTCACTCT AGAGTGGATC 
CCTGGCTTCA ATGGGGGTTT GCAGCAGAGA TATCGCATAC GATACATCTG GGACCAGTCT 
GTCAGCTACA TGTATGTAGA TGTCGTTCCC CCTGACACAA CAACCTTCAC CGTTACGGGT 
CTGCAGCCGC AGACCACCTA CAACTTCTCA GTTAATGCCC TAAACTCCAT AGGAGAGAGT 
GGCTATGCTG ACGACGGTGC AGTACTGACC ATCACCACTG AGGTGAAGAA AGATATAGAA 
GAGGATCGAT CGGATGATGA CACACAGGCT CCGAGTGGCC TGCCAACCCA TTTGACTGTG 
GCCTTCACAG TGGTGTTCGG AGTCCTGCTG GTGTTGAATT CGCTGGGCTG CTTCTTTGGG 
CGGAAGTGGA AAAACAGGCG TGACAAAACA AAGGCAGAAA GGGACAGTGT GGTGAATGGA 
CAGAAGAATG AAGAGGATGT GTCAAGTCAA TCAACTGGCA TCTCCAATAA GTACGAGAGC 
AGAGAAAAAA TCAACACCGG AGCCCAACGT ACCCTCCTCG TTGACACTGG ATCTGAAACA 
GAGAGCAACG TCTACGAGAG CTACACAGCG TTTCAGGAAG ACTCCCATTA TTATTATCCT 
ACTGGTGACT ATAAACCGTA CCATGGTCCA CATCCTGAGG ACAGACATGG CCTTGGTGCC 
GCTCCTGTGA ACTTTGAAAG CCATTTGTAT GAAGATGTGG GTGATAGACG AGTGTACCAG 
GACACACTGC CTTCCTCTCT TCCTGCCTAC CGTCCACTTT TCTCTGATCG CAGACTGATC 
CAGCAAGCAG AGGACTGGAG GTCACCAACC GACCCTCAGG GTATCAAGAG AGAAGGGCAG 
TTCCCGGATA ATCAACAACC GGAGCGGGAT TATGATCTAC CCTTCGAGTT GCGAGGAGAA 
TTAGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nphs1 ( XM_007559501.2 ) cDNA ORF clone, Poecilia formosa(Amazon molly) -> XP_007559563.2 Poecilia formosa NPHS1 nephrin (nphs1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPo142678
Clone ID Related Accession (Same CDS sequence)	XM_007559501.2
Accession Version	XM_007559501.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3612bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-05-02
Organism	Poecilia formosa(Amazon molly)
Product	nephrin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006800044.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 3, 2016 this sequence version replaced XM_007559501.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia formosa Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGCCA CAGCTGTGCT GAAGTGTGAG GTCCTCAGGG CCTCGGGGAT TGTGCAGTGG 
GCGAAAGACG GCTTTCTTTT GGGACCTCAG AGAAGCCTGC CAGGCTTCCC ACGCTACAGC 
ATGATTGGAA GGAGAGGACA GTATTATCTC AGGATTGAAG ATGCACAACT TGATGATGAC 
GCCTTCTTTG AATGCCAAGC CGGCCAATCC GAGAGAAGTG ATGCTATTGT CTCCAATCCA 
GCCTTTCTCA CCGTGCAAAT TCCCCCATCG AAGCCGTATT TTACCACGGA TGTGACAACA 
CCCTGGGTGG CGGGAAAGAA GTACATGGTT GAATGCGTGG CCCCTGACGC AAAGCCTAGT 
GCTGATATCA CTCTGTATAA AGATGGAGTC GATCTGACAG GTGCGACGTC TTTTACCACA 
TCTGGCTCAA AGGATAAGCT TCTGAACACG CACGCCGAAG TAACGGTCAC TGCTCTGAGA 
TCTGACAATG GGAGAGAGTT GACATGTCAC GCCAACAATG AAGCCGTTTC CCAGCCGGTG 
GTGACCACAG TGACCATGAA TGTGTATTAT CCTCCTGAGC CTCCATATAT TATTGGTTTG 
GACAGAGACG TGGTTAAAGC GGGGAGTTTG CTCGTGTTGG AGTGTGTATC CCATGGTGGA 
AACCCCCTGG GCACTCTTCA ATGGACTAAG GAAGGCGAAG TTCTCTCAAC AACCTGGGGA 
GAGGAAGTCG TGGGCCAGAA GTCCAGAAAT ATCCTCAGAC TACAGATCAC CCCTGCTGAC 
AACCAGGCAG AGCTGAGCTG TGAAAGTGCC AATATTGTCT CCCCGTCTCC TCTGTCCGTC 
ACCCGCAAAA TAACAGTGTT CTTCGGTCCT GCGACGCTGA CGCGGTCGGG TTCGCTGGAG 
GCCATCGAGG GGCAGGAGTT GACTCTGAGC TGCCATGCGT CCTCGAGTAA TCCCCCCGTC 
CACATTCGCT GGTGGCTGGG GCACAAGGAG CTCAGCAACA CCTCTGTCAC AATGGAAGAG 
GGAGAGAACG GAGGGATGAC AGCCACCTCC AATGTGACCC ACCGGGTCTC TCGGGAGGAG 
AATGGACTGA AGCTTACCTG TGAGGCTTTT AATAACGGCA CACATCTCTC CAAAATCCAG 
GCCGCTAAGC TCACTGTGTA CTATCCTCCT CAAAAAGTTT TTATTGATTC GCCGCCCGAG 
GACATCCCTC TGCGCTCGGG CAGCAGAGCC GTGCTTGTCT GCCACTCGGC TGGAGGGAAT 
CCTCCTGCAA AGCTCACCTG GTTTAAGAAT GGAAAAAAGG TGTCAGACGC AGAGCCGCAG 
ACGTCCTCTA ATATGGTTGT AACTCGGGTG CTCAACCTAC TGCTGACGGC GAGCGACAAC 
CAGGCTGCCT ACCGCTGTGA CGCCACAAAT GAGGCGAAGA GAACGATATC AGCACATGCT 
AAGCTACTGG TCCAGTTTAC AGCCGTGACT ATGAAGATTA CAGCCAAAGA AGAAGTGTTT 
TACCGAGGTC AGCAGATGGA ACTGGAGTGT CTTTCCGGGA GCAGCAACCC AAAGTCTGAC 
ATTTCCTGGA AAGTGGGCCC ACTGAGATTG CAGGGAGTTG AGGAGGCACC CAAGGAGGCC 
GAGTTTGGGG GCGTTTCTGT GCTCAGTAGG CTTCACCTGA ATCTGACTTC ACAGCACCAC 
AACCAGAAGG TGATCTGTCA GGCCTACAGC CCTGTGTTGG AAGAAGGAGC TAGTACGTTT 
TACCAACTGA ATGTGATATA TCCTCCTGAA TTCAGCTCTG AGCAAGCAAC AGAAGTCCAG 
GTGGTGGAGG ACGATGTGGC AACCATCCCC CTGATGGTCT CAGCAAACCC CGAGGTGGTC 
TCCTGCATTT GGCTGCGCCG CAAGCAGATG CTGGTCAAAG GGGAAGACAT ACGCCACCAC 
TGGTCAGATG ACCACTCTCT GGAGATTAGG AATGTGACGA GAAAAGACAA AGGACTTTAC 
ACTATCAAAT GTGAAAATGG CGAAGGCGTC AACGAAACCA CTGTTAAGCT GGATGTTTAC 
TATGCTCCGA CTGTCAAGGC GGAGAAAGAC CCTGTGTATG TTAACCAGGG GGGAACAGCA 
GACCTGATAT GTGTGGCAGA TGCCAACCCC ATTATACCGG AGATGTTCTC TTGGAAATGG 
CTGGGAGAGG AAGAAGTGGA GATGGGAGAG GAGACCAACG AAAACAAATC AAGCTTGCTG 
ACCATTCATA ATGTGACTCG GGCTCACGCT GGTTTTTACC AGTGTTCGGC CAGCAATGGC 
ATAGCACTCC CTGGCAGTGT GGAAATGAGG CTGGTGGTGC AATTCATTCC GGAGCTTCGG 
AAAGGACCCC AGTGGAGGAA GGTTGCCAGT CGAGGGGACG GCACTGCCAC TGCTGAGCTG 
GTGTGTCAGG CAGAGGGCAT TCCTCCTGTA GAGTTTACTT GGGAAAAGAA AGGGATGCTC 
ATAGATTTGG CAAACCCCAG GTATGAGGAG AGGACAGCGA GGGATGGCTC TTTCCACACC 
AGTACTCTGC GAGTGGTGAA TGTGAGCGCC ACTCTGGACT ACGCCGTCTT CAGCTGCACT 
GCTCGCAACT CACTTGGGGA AGACAAGCTA GACATCCAGC TCGTCAGCAC AAATCACCCA 
GATCCACCTT CATCATTTAG TCTAGTCGGT GTTACCCATA ACTCCGTCAC TCTAGAGTGG 
ATCCCTGGCT TCAATGGGGG TTTGCAGCAG AGATATCGCA TACGATACAT CTGGGACCAG 
TCTGTCAGCT ACATGTATGT AGATGTCGTT CCCCCTGACA CAACAACCTT CACCGTTACG 
GGTCTGCAGC CGCAGACCAC CTACAACTTC TCAGTTAATG CCCTAAACTC CATAGGAGAG 
AGTGGCTATG CTGACGACGG TGCAGTACTG ACCATCACCA CTGAGGTGAA GAAAGATATA 
GAAGAGGATC GATCGGATGA TGACACACAG GCTCCGAGTG GCCTGCCAAC CCATTTGACT 
GTGGCCTTCA CAGTGGTGTT CGGAGTCCTG CTGGTGTTGA ATTCGCTGGG CTGCTTCTTT 
GGGCGGAAGT GGAAAAACAG GCGTGACAAA ACAAAGGCAG AAAGGGACAG TGTGGTGAAT 
GGACAGAAGA ATGAAGAGGA TGTGTCAAGT CAATCAACTG GCATCTCCAA TAAGTACGAG 
AGCAGAGAAA AAATCAACAC CGGAGCCCAA CGTACCCTCC TCGTTGACAC TGGATCTGAA 
ACAGAGAGCA ACGTCTACGA GAGCTACACA GCGTTTCAGG AAGACTCCCA TTATTATTAT 
CCTACTGGTG ACTATAAACC GTACCATGGT CCACATCCTG AGGACAGACA TGGCCTTGGT 
GCCGCTCCTG TGAACTTTGA AAGCCATTTG TATGAAGATG TGGGTGATAG ACGAGTGTAC 
CAGGACACAC TGCCTTCCTC TCTTCCTGCC TACCGTCCAC TTTTCTCTGA TCGCAGACTG 
ATCCAGCAAG CAGAGGACTG GAGGTCACCA ACCGACCCTC AGGGTATCAA GAGAGAAGGG 
CAGTTCCCGG ATAATCAACA ACCGGAGCGG GATTATGATC TACCCTTCGA GTTGCGAGGA 
GAATTAGTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007559563.2
CDS	142..3753
Translation	MGATAVLKCEVLRASGIVQWAKDGFLLGPQRSLPGFPRYSMIGRRGQYYLRIEDAQLDDDAFFECQAGQSERSDAIVSNPAFLTVQIPPSKPYFTTDVTTPWVAGKKYMVECVAPDAKPSADITLYKDGVDLTGATSFTTSGSKDKLLNTHAEVTVTALRSDNGRELTCHANNEAVSQPVVTTVTMNVYYPPEPPYIIGLDRDVVKAGSLLVLECVSHGGNPLGTLQWTKEGEVLSTTWGEEVVGQKSRNILRLQITPADNQAELSCESANIVSPSPLSVTRKITVFFGPATLTRSGSLEAIEGQELTLSCHASSSNPPVHIRWWLGHKELSNTSVTMEEGENGGMTATSNVTHRVSREENGLKLTCEAFNNGTHLSKIQAAKLTVYYPPQKVFIDSPPEDIPLRSGSRAVLVCHSAGGNPPAKLTWFKNGKKVSDAEPQTSSNMVVTRVLNLLLTASDNQAAYRCDATNEAKRTISAHAKLLVQFTAVTMKITAKEEVFYRGQQMELECLSGSSNPKSDISWKVGPLRLQGVEEAPKEAEFGGVSVLSRLHLNLTSQHHNQKVICQAYSPVLEEGASTFYQLNVIYPPEFSSEQATEVQVVEDDVATIPLMVSANPEVVSCIWLRRKQMLVKGEDIRHHWSDDHSLEIRNVTRKDKGLYTIKCENGEGVNETTVKLDVYYAPTVKAEKDPVYVNQGGTADLICVADANPIIPEMFSWKWLGEEEVEMGEETNENKSSLLTIHNVTRAHAGFYQCSASNGIALPGSVEMRLVVQFIPELRKGPQWRKVASRGDGTATAELVCQAEGIPPVEFTWEKKGMLIDLANPRYEERTARDGSFHTSTLRVVNVSATLDYAVFSCTARNSLGEDKLDIQLVSTNHPDPPSSFSLVGVTHNSVTLEWIPGFNGGLQQRYRIRYIWDQSVSYMYVDVVPPDTTTFTVTGLQPQTTYNFSVNALNSIGESGYADDGAVLTITTEVKKDIEEDRSDDDTQAPSGLPTHLTVAFTVVFGVLLVLNSLGCFFGRKWKNRRDKTKAERDSVVNGQKNEEDVSSQSTGISNKYESREKINTGAQRTLLVDTGSETESNVYESYTAFQEDSHYYYPTGDYKPYHGPHPEDRHGLGAAPVNFESHLYEDVGDRRVYQDTLPSSLPAYRPLFSDRRLIQQAEDWRSPTDPQGIKREGQFPDNQQPERDYDLPFELRGELV

Target ORF information:

RefSeq Version	XM_007559501.2
Organism	Poecilia formosa(Amazon molly)
Definition	Poecilia formosa NPHS1 nephrin (nphs1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007559501.2

ORF Insert Sequence:

ATGGGAGCCA CAGCTGTGCT GAAGTGTGAG GTCCTCAGGG CCTCGGGGAT TGTGCAGTGG 
GCGAAAGACG GCTTTCTTTT GGGACCTCAG AGAAGCCTGC CAGGCTTCCC ACGCTACAGC 
ATGATTGGAA GGAGAGGACA GTATTATCTC AGGATTGAAG ATGCACAACT TGATGATGAC 
GCCTTCTTTG AATGCCAAGC CGGCCAATCC GAGAGAAGTG ATGCTATTGT CTCCAATCCA 
GCCTTTCTCA CCGTGCAAAT TCCCCCATCG AAGCCGTATT TTACCACGGA TGTGACAACA 
CCCTGGGTGG CGGGAAAGAA GTACATGGTT GAATGCGTGG CCCCTGACGC AAAGCCTAGT 
GCTGATATCA CTCTGTATAA AGATGGAGTC GATCTGACAG GTGCGACGTC TTTTACCACA 
TCTGGCTCAA AGGATAAGCT TCTGAACACG CACGCCGAAG TAACGGTCAC TGCTCTGAGA 
TCTGACAATG GGAGAGAGTT GACATGTCAC GCCAACAATG AAGCCGTTTC CCAGCCGGTG 
GTGACCACAG TGACCATGAA TGTGTATTAT CCTCCTGAGC CTCCATATAT TATTGGTTTG 
GACAGAGACG TGGTTAAAGC GGGGAGTTTG CTCGTGTTGG AGTGTGTATC CCATGGTGGA 
AACCCCCTGG GCACTCTTCA ATGGACTAAG GAAGGCGAAG TTCTCTCAAC AACCTGGGGA 
GAGGAAGTCG TGGGCCAGAA GTCCAGAAAT ATCCTCAGAC TACAGATCAC CCCTGCTGAC 
AACCAGGCAG AGCTGAGCTG TGAAAGTGCC AATATTGTCT CCCCGTCTCC TCTGTCCGTC 
ACCCGCAAAA TAACAGTGTT CTTCGGTCCT GCGACGCTGA CGCGGTCGGG TTCGCTGGAG 
GCCATCGAGG GGCAGGAGTT GACTCTGAGC TGCCATGCGT CCTCGAGTAA TCCCCCCGTC 
CACATTCGCT GGTGGCTGGG GCACAAGGAG CTCAGCAACA CCTCTGTCAC AATGGAAGAG 
GGAGAGAACG GAGGGATGAC AGCCACCTCC AATGTGACCC ACCGGGTCTC TCGGGAGGAG 
AATGGACTGA AGCTTACCTG TGAGGCTTTT AATAACGGCA CACATCTCTC CAAAATCCAG 
GCCGCTAAGC TCACTGTGTA CTATCCTCCT CAAAAAGTTT TTATTGATTC GCCGCCCGAG 
GACATCCCTC TGCGCTCGGG CAGCAGAGCC GTGCTTGTCT GCCACTCGGC TGGAGGGAAT 
CCTCCTGCAA AGCTCACCTG GTTTAAGAAT GGAAAAAAGG TGTCAGACGC AGAGCCGCAG 
ACGTCCTCTA ATATGGTTGT AACTCGGGTG CTCAACCTAC TGCTGACGGC GAGCGACAAC 
CAGGCTGCCT ACCGCTGTGA CGCCACAAAT GAGGCGAAGA GAACGATATC AGCACATGCT 
AAGCTACTGG TCCAGTTTAC AGCCGTGACT ATGAAGATTA CAGCCAAAGA AGAAGTGTTT 
TACCGAGGTC AGCAGATGGA ACTGGAGTGT CTTTCCGGGA GCAGCAACCC AAAGTCTGAC 
ATTTCCTGGA AAGTGGGCCC ACTGAGATTG CAGGGAGTTG AGGAGGCACC CAAGGAGGCC 
GAGTTTGGGG GCGTTTCTGT GCTCAGTAGG CTTCACCTGA ATCTGACTTC ACAGCACCAC 
AACCAGAAGG TGATCTGTCA GGCCTACAGC CCTGTGTTGG AAGAAGGAGC TAGTACGTTT 
TACCAACTGA ATGTGATATA TCCTCCTGAA TTCAGCTCTG AGCAAGCAAC AGAAGTCCAG 
GTGGTGGAGG ACGATGTGGC AACCATCCCC CTGATGGTCT CAGCAAACCC CGAGGTGGTC 
TCCTGCATTT GGCTGCGCCG CAAGCAGATG CTGGTCAAAG GGGAAGACAT ACGCCACCAC 
TGGTCAGATG ACCACTCTCT GGAGATTAGG AATGTGACGA GAAAAGACAA AGGACTTTAC 
ACTATCAAAT GTGAAAATGG CGAAGGCGTC AACGAAACCA CTGTTAAGCT GGATGTTTAC 
TATGCTCCGA CTGTCAAGGC GGAGAAAGAC CCTGTGTATG TTAACCAGGG GGGAACAGCA 
GACCTGATAT GTGTGGCAGA TGCCAACCCC ATTATACCGG AGATGTTCTC TTGGAAATGG 
CTGGGAGAGG AAGAAGTGGA GATGGGAGAG GAGACCAACG AAAACAAATC AAGCTTGCTG 
ACCATTCATA ATGTGACTCG GGCTCACGCT GGTTTTTACC AGTGTTCGGC CAGCAATGGC 
ATAGCACTCC CTGGCAGTGT GGAAATGAGG CTGGTGGTGC AATTCATTCC GGAGCTTCGG 
AAAGGACCCC AGTGGAGGAA GGTTGCCAGT CGAGGGGACG GCACTGCCAC TGCTGAGCTG 
GTGTGTCAGG CAGAGGGCAT TCCTCCTGTA GAGTTTACTT GGGAAAAGAA AGGGATGCTC 
ATAGATTTGG CAAACCCCAG GTATGAGGAG AGGACAGCGA GGGATGGCTC TTTCCACACC 
AGTACTCTGC GAGTGGTGAA TGTGAGCGCC ACTCTGGACT ACGCCGTCTT CAGCTGCACT 
GCTCGCAACT CACTTGGGGA AGACAAGCTA GACATCCAGC TCGTCAGCAC AAATCACCCA 
GATCCACCTT CATCATTTAG TCTAGTCGGT GTTACCCATA ACTCCGTCAC TCTAGAGTGG 
ATCCCTGGCT TCAATGGGGG TTTGCAGCAG AGATATCGCA TACGATACAT CTGGGACCAG 
TCTGTCAGCT ACATGTATGT AGATGTCGTT CCCCCTGACA CAACAACCTT CACCGTTACG 
GGTCTGCAGC CGCAGACCAC CTACAACTTC TCAGTTAATG CCCTAAACTC CATAGGAGAG 
AGTGGCTATG CTGACGACGG TGCAGTACTG ACCATCACCA CTGAGGTGAA GAAAGATATA 
GAAGAGGATC GATCGGATGA TGACACACAG GCTCCGAGTG GCCTGCCAAC CCATTTGACT 
GTGGCCTTCA CAGTGGTGTT CGGAGTCCTG CTGGTGTTGA ATTCGCTGGG CTGCTTCTTT 
GGGCGGAAGT GGAAAAACAG GCGTGACAAA ACAAAGGCAG AAAGGGACAG TGTGGTGAAT 
GGACAGAAGA ATGAAGAGGA TGTGTCAAGT CAATCAACTG GCATCTCCAA TAAGTACGAG 
AGCAGAGAAA AAATCAACAC CGGAGCCCAA CGTACCCTCC TCGTTGACAC TGGATCTGAA 
ACAGAGAGCA ACGTCTACGA GAGCTACACA GCGTTTCAGG AAGACTCCCA TTATTATTAT 
CCTACTGGTG ACTATAAACC GTACCATGGT CCACATCCTG AGGACAGACA TGGCCTTGGT 
GCCGCTCCTG TGAACTTTGA AAGCCATTTG TATGAAGATG TGGGTGATAG ACGAGTGTAC 
CAGGACACAC TGCCTTCCTC TCTTCCTGCC TACCGTCCAC TTTTCTCTGA TCGCAGACTG 
ATCCAGCAAG CAGAGGACTG GAGGTCACCA ACCGACCCTC AGGGTATCAA GAGAGAAGGG 
CAGTTCCCGG ATAATCAACA ACCGGAGCGG GATTATGATC TACCCTTCGA GTTGCGAGGA 
GAATTAGTCT AG