themis2 cDNA ORF clone, Poecilia formosa(Amazon molly)

Gene
Clones
gRNAs

Summary

Gene Symbol	themis2
Entrez Gene ID	103141565
Full Name	thymocyte selection associated family member 2
Gene Type	protein-coding
Organism	Poecilia formosa(Amazon molly)

mRNA and Protein(s)

mRNA	Protein	Name
XM_007557257.2	XP_007557319.1	protein THEMIS2

Pathways from BioSystems
Gene Ontology

The following themis2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the themis2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPo140729	XM_007557257.2 Latest version!	Poecilia formosa thymocyte selection associated family member 2 (themis2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

themis2 ( XM_007557257.2 ) cDNA ORF clone, Poecilia formosa(Amazon molly) -> XP_007557319.1 Poecilia formosa thymocyte selection associated family member 2 (themis2), mRNA.

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CloneID	OPo140729
Clone ID Related Accession (Same CDS sequence)	XM_007557257.2
Accession Version	XM_007557257.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-05-02
Organism	Poecilia formosa(Amazon molly)
Product	protein THEMIS2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006800025.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 3, 2016 this sequence version replaced XM_007557257.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia formosa Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGCA ACATTGCTTT GCCGCTTCAA GAATTCATCG CTTCACTGGA CAACACCAGT 
TTGCCAAAAA TTTTGCAAGT TTGTTCTGGA GTGTATTTCC AAGGTTCGAT ATATGAAATT 
TCTGGAAGTG AAGTGTGCTT TTCTACTGGG GATCTCATAA AGGTCATTGG TATTGAACTG 
CAATCTGTTT CCTGTGAAGA TGTCACTAAC AATGAGAAGT TTGAGTTGCC AGTCACTCAC 
ACAGGATTGT TCAAAGTGGT CCCCGAGCAG ATGCCGTACA CGTCAGTCGA GGAGATGTTG 
AGTCTGAGGC CCGTGGACCT GGATGATTCC CTTCCTTTTA CCTTCGTCAG CCACTCCAAG 
GTGACCTTCG GCAGTCTCAC GCTGGGGGCC GGCAAAGCGC TGACTGTGCT CTCAATCGAG 
AAGCGTGAAG ATGAAGAGGA TATGGTGCGT TGCCATGTAA AAGGCCAAAC GGAGGCCTCA 
GCTGAGGTGT GCATTCCTCT GTCTTCTCGA GGGGAATTTT CTGAATGCGA GAGTGAGGAA 
TCCTACACCC TGAAGGAGAT CATGGCTTCT CCTTGCCTGC GCAGTCGAAG ATTTCGCTTT 
GTTAAGACCA CCAAGTGTGA GCGGATCCTT GTCCTGAGTC CAGTCTATCA AGTCCAGGCC 
ATCATGAACA TGAGGAAGAA CGTACTAAGG TTTCCGTCCA GCCTGGAGGT GGATGTGGTC 
GATGTCACTG ATGTGTGTAA AGATGCAAAC TTTGTGACAC CTCTAAACCT GACAGAGGTC 
CTGTCTCAGC CAGACAAATC ATTTCCTGTG GTGGCAGAGA TATTAGAGGA GCCAGAGAGC 
CGCTCTTTCT TCAAGTGCGC GTGGCTGCCA AGGCTGGTCA AGAGCACCAA CCTGATTTTG 
CACAAGAAAG GAACTACGCC CATGGTCCTG TTGTCCAGCT TGAAGGGCCG GAAGACTCAG 
CAGTACTTCC TTGTGTCCCA GCAGTACGGT GGACGGTTTC GGAGGAGGCC AAGAGAGTTC 
AACTCGGTGT ACGAGGTGTA CGTTGCTTCA ACGCAGACAC ACAGCCTGAC GGTGAGCGTG 
ACGAGGAACT GTGAGGAAGT CGAGGAGGAG GGTCTGCCAG GCCTCAGCGT TGGGGAGAGG 
CTACAGATTG TGGACTGCAC TAGAATGGAG TTGCCTTCTG AAAACAATAA AGGGGAGAAG 
CAGTCTGTCG AGGCTCTTTT GTGTCATCGT CTCCAAGAGC CAGACGACGA GGAGGACGAC 
GAAGAGGATG AGGATGAAGA GAAGGATAAA CAGGAGGAGG CAAAAGAGGA GGTCTTACTG 
CCTCTCTACA TGCAGGGTCA CTTTGTAGAG GTTCTTGGTG AAAACAAAAA GTATAAACTC 
TGTGATTTGG GTAAGGAGTT CAGTTTGCCA CTGGATGTCA AAGTGGTGAG TCGAGACACT 
GACCTAGAGA CTGATCCACT AGTTGGGTTT CCTTGTCTGA GGATAGAGGG AGCAATGCTG 
GAGCCAAGCA TTCAGGCAAG CTTTACAGAC AAACCCGACC ACTGTTTCCA TCTCCTCCCA 
CAGTGGCTCT CTTTGTCCGT CTCTTTTACC AAAGATGCTC TTCCTTGGCC TCGGGACCAA 
TTCCCCAAGT GTTTCACGGA AAAGGTTAGT GAGATGACAG ACACGTTCTT TTATGAATTT 
CGCAAAAAGG GTAACTCGGA CGAGGCTCCT CCACCTCGAC CGCCAAAACG AGACATGTCA 
TCATCAAACT TAGTCACAAA AAAATCTAAA TCCACAAAGA AATCCTCCAA GCCAAAGAAA 
AACAAAGACA AAAGTGTACT GACGGAGAAG CTCAATGACT TGACGTTGAA TCACAAAAAA 
CGGCCCCCGG CTCCACTGCC TCCAGATGAA CCTCCTCCAC TTGTACCGCG AAAGTGCTCA 
GCCGCAGATA TCACAAGCAA GGCCAAGCCA AACACTTATG TAAAGATAAA CCAACCATTA 
AAAAGGGACC ACCTGTGTGA TGTGGCTGCA GACGTGGAAA GTGACCATGA CTACGAAACC 
GTGGATGAGA CTTTGGTGGC TGTGGTTAAG ACAGCTCAAG AAAACGTGAT GTTCTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007557319.1
CDS	20..2119
Translation	MAGNIALPLQEFIASLDNTSLPKILQVCSGVYFQGSIYEISGSEVCFSTGDLIKVIGIELQSVSCEDVTNNEKFELPVTHTGLFKVVPEQMPYTSVEEMLSLRPVDLDDSLPFTFVSHSKVTFGSLTLGAGKALTVLSIEKREDEEDMVRCHVKGQTEASAEVCIPLSSRGEFSECESEESYTLKEIMASPCLRSRRFRFVKTTKCERILVLSPVYQVQAIMNMRKNVLRFPSSLEVDVVDVTDVCKDANFVTPLNLTEVLSQPDKSFPVVAEILEEPESRSFFKCAWLPRLVKSTNLILHKKGTTPMVLLSSLKGRKTQQYFLVSQQYGGRFRRRPREFNSVYEVYVASTQTHSLTVSVTRNCEEVEEEGLPGLSVGERLQIVDCTRMELPSENNKGEKQSVEALLCHRLQEPDDEEDDEEDEDEEKDKQEEAKEEVLLPLYMQGHFVEVLGENKKYKLCDLGKEFSLPLDVKVVSRDTDLETDPLVGFPCLRIEGAMLEPSIQASFTDKPDHCFHLLPQWLSLSVSFTKDALPWPRDQFPKCFTEKVSEMTDTFFYEFRKKGNSDEAPPPRPPKRDMSSSNLVTKKSKSTKKSSKPKKNKDKSVLTEKLNDLTLNHKKRPPAPLPPDEPPPLVPRKCSAADITSKAKPNTYVKINQPLKRDHLCDVAADVESDHDYETVDETLVAVVKTAQENVMFY

Target ORF information:

RefSeq Version	XM_007557257.2
Organism	Poecilia formosa(Amazon molly)
Definition	Poecilia formosa thymocyte selection associated family member 2 (themis2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007557257.2

ORF Insert Sequence:

ATGGCAGGCA ACATTGCTTT GCCGCTTCAA GAATTCATCG CTTCACTGGA CAACACCAGT 
TTGCCAAAAA TTTTGCAAGT TTGTTCTGGA GTGTATTTCC AAGGTTCGAT ATATGAAATT 
TCTGGAAGTG AAGTGTGCTT TTCTACTGGG GATCTCATAA AGGTCATTGG TATTGAACTG 
CAATCTGTTT CCTGTGAAGA TGTCACTAAC AATGAGAAGT TTGAGTTGCC AGTCACTCAC 
ACAGGATTGT TCAAAGTGGT CCCCGAGCAG ATGCCGTACA CGTCAGTCGA GGAGATGTTG 
AGTCTGAGGC CCGTGGACCT GGATGATTCC CTTCCTTTTA CCTTCGTCAG CCACTCCAAG 
GTGACCTTCG GCAGTCTCAC GCTGGGGGCC GGCAAAGCGC TGACTGTGCT CTCAATCGAG 
AAGCGTGAAG ATGAAGAGGA TATGGTGCGT TGCCATGTAA AAGGCCAAAC GGAGGCCTCA 
GCTGAGGTGT GCATTCCTCT GTCTTCTCGA GGGGAATTTT CTGAATGCGA GAGTGAGGAA 
TCCTACACCC TGAAGGAGAT CATGGCTTCT CCTTGCCTGC GCAGTCGAAG ATTTCGCTTT 
GTTAAGACCA CCAAGTGTGA GCGGATCCTT GTCCTGAGTC CAGTCTATCA AGTCCAGGCC 
ATCATGAACA TGAGGAAGAA CGTACTAAGG TTTCCGTCCA GCCTGGAGGT GGATGTGGTC 
GATGTCACTG ATGTGTGTAA AGATGCAAAC TTTGTGACAC CTCTAAACCT GACAGAGGTC 
CTGTCTCAGC CAGACAAATC ATTTCCTGTG GTGGCAGAGA TATTAGAGGA GCCAGAGAGC 
CGCTCTTTCT TCAAGTGCGC GTGGCTGCCA AGGCTGGTCA AGAGCACCAA CCTGATTTTG 
CACAAGAAAG GAACTACGCC CATGGTCCTG TTGTCCAGCT TGAAGGGCCG GAAGACTCAG 
CAGTACTTCC TTGTGTCCCA GCAGTACGGT GGACGGTTTC GGAGGAGGCC AAGAGAGTTC 
AACTCGGTGT ACGAGGTGTA CGTTGCTTCA ACGCAGACAC ACAGCCTGAC GGTGAGCGTG 
ACGAGGAACT GTGAGGAAGT CGAGGAGGAG GGTCTGCCAG GCCTCAGCGT TGGGGAGAGG 
CTACAGATTG TGGACTGCAC TAGAATGGAG TTGCCTTCTG AAAACAATAA AGGGGAGAAG 
CAGTCTGTCG AGGCTCTTTT GTGTCATCGT CTCCAAGAGC CAGACGACGA GGAGGACGAC 
GAAGAGGATG AGGATGAAGA GAAGGATAAA CAGGAGGAGG CAAAAGAGGA GGTCTTACTG 
CCTCTCTACA TGCAGGGTCA CTTTGTAGAG GTTCTTGGTG AAAACAAAAA GTATAAACTC 
TGTGATTTGG GTAAGGAGTT CAGTTTGCCA CTGGATGTCA AAGTGGTGAG TCGAGACACT 
GACCTAGAGA CTGATCCACT AGTTGGGTTT CCTTGTCTGA GGATAGAGGG AGCAATGCTG 
GAGCCAAGCA TTCAGGCAAG CTTTACAGAC AAACCCGACC ACTGTTTCCA TCTCCTCCCA 
CAGTGGCTCT CTTTGTCCGT CTCTTTTACC AAAGATGCTC TTCCTTGGCC TCGGGACCAA 
TTCCCCAAGT GTTTCACGGA AAAGGTTAGT GAGATGACAG ACACGTTCTT TTATGAATTT 
CGCAAAAAGG GTAACTCGGA CGAGGCTCCT CCACCTCGAC CGCCAAAACG AGACATGTCA 
TCATCAAACT TAGTCACAAA AAAATCTAAA TCCACAAAGA AATCCTCCAA GCCAAAGAAA 
AACAAAGACA AAAGTGTACT GACGGAGAAG CTCAATGACT TGACGTTGAA TCACAAAAAA 
CGGCCCCCGG CTCCACTGCC TCCAGATGAA CCTCCTCCAC TTGTACCGCG AAAGTGCTCA 
GCCGCAGATA TCACAAGCAA GGCCAAGCCA AACACTTATG TAAAGATAAA CCAACCATTA 
AAAAGGGACC ACCTGTGTGA TGTGGCTGCA GACGTGGAAA GTGACCATGA CTACGAAACC 
GTGGATGAGA CTTTGGTGGC TGTGGTTAAG ACAGCTCAAG AAAACGTGAT GTTCTATTAA