LOC103140231 cDNA ORF clone, Poecilia formosa(Amazon molly)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC103140231
Entrez Gene ID	103140231
Full Name	muscarinic acetylcholine receptor M2
Synonyms	chrm2
Gene Type	protein-coding
Organism	Poecilia formosa(Amazon molly)

mRNA and Protein(s)

mRNA	Protein	Name
XM_007555260.2	XP_007555322.1	muscarinic acetylcholine receptor M2

Pathways from BioSystems
Gene Ontology

The following LOC103140231 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC103140231 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPo139005	XM_007555260.2 Latest version!	Poecilia formosa muscarinic acetylcholine receptor M2 (LOC103140231), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC103140231 ( XM_007555260.2 ) cDNA ORF clone, Poecilia formosa(Amazon molly) -> XP_007555322.1 Poecilia formosa muscarinic acetylcholine receptor M2 (LOC103140231), mRNA.

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CloneID	OPo139005
Clone ID Related Accession (Same CDS sequence)	XM_007555260.2
Accession Version	XM_007555260.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1560bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-05-02
Organism	Poecilia formosa(Amazon molly)
Product	muscarinic acetylcholine receptor M2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006800008.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 3, 2016 this sequence version replaced XM_007555260.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia formosa Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTCTG TTCCAACCGA CTTGTACGTG GCAACAAAGA AACCAAATGC CATGGATGTC 
TTTAACTTTA CATCCTGGAA TGCCTCTGAA GGCAATGAGA CAGAAGTCAT GGAGGAGAGA 
GACAGTCCTT ACAAGACCGT GGAGGTAGTG TTCATCGTGC TGGTGGCTGG TTCCCTGAGT 
TTGGTCACTA TCATCGGCAA TATCCTGGTC ATGCTTTCCA TCAAGGTGAA CAGGAACCTA 
CAAACTGTCA ACAACTATTT TTTATTTAGC CTTGCATGTG CTGACCTAAT TATTGGACTG 
TGCTCCATGA ACTTGTACAC AGTATATATA GTAATAGGCT ACTGGCCCCT GGGGCCAGTC 
GTGTGCGACT TGTGGTTGGC TTTGGACTAT GTTGTCAGCA ATGCGTCTGT CATGAATCTC 
CTCATCATAA GCTTTGACAG ATACTTCTGT GTCACCAAGC CCCTGAGCTA CCCTGTAAAG 
AGAACCACCA AAATGGCAGG GATGATGATC GCTGCTGCCT GGGTCCTTTC CTTCATCCTC 
TGGGCACCTG CTATCCTCTT CTGGCAGTTC ATTGTTGGTG GGCGGACTGT GCCTGAGAAA 
GAGTGCTACA TTCAGTTCTT TTCCAATGCC GCTGTTACTT TTGGAACTGC CATTGCCGCC 
TTTTACCTGC CTGTCATTAT CATGATTCAG CTGTACTGGC AGATCTCGCG AGCGAGCAAA 
AGTCGAGTGA AGAAGGACAA CCGCAAGCCT TCAGGATCAA ACCCAGAACC TTTGTTACCG 
GGCCAGAGGA GAGAGAGCAC TCCGAAACCC AACAACAACA ATGTTCCTGG GGAAGACGGA 
GCGCATTCCC AGAACCAGAC TGCAGATGAT GGAGCCAACC AGCACGATGG AAAGCTGCAG 
AATGGAAAAG GGCCTTCATC GTCTGCTGCC GACGTAGAAA CGGAGGGTGA CAGTGTTGCA 
AAGGAGAACT GTGCTCCTGC AGAAGAGAAG GAGAGCTCAA ATGACTCCAC GTCCGGCAGT 
GTGGCAGCTT CCAATCAGAA GGACGAAGAA CAAGTCGCCT CTGCAGCAAA CTCCAGTGCT 
GAGGCCACGC AGCAGCTGCC ACGGCAACGA GCCAAGGCTG GGGGCTCCAA GCTGACCTGC 
ATCAAGATCA AGACCAAGTC CCCGAAGGGC GACTACTACA CACCGTCAAA CGCCACAGTT 
GAGATCGTGC CAACCACAGA ACGTCAGAAC CACGTGGCGA GGAAGATCGT GAAGATGACG 
AAGCAACCGC CAAACAAGAA GAAGAAAGGA GCCCCGTCGC GGGAGAAAAA AGTGACCCGC 
ACGATAATGG CCATCCTGGT GGCTTTCGTA GCTACCTGGA CTCCGTATAA CGTGATGGTG 
CTCATCAATA CTTTTTGTTC TAGTTGCATT CCCAACACTG TTTGGACTAT TGGTTACTGG 
CTGTGCTACA TCAACAGCAC CATCAACCCA GCCTGCTATG CTCTGTGTAA TGTCACGTTC 
AAAAAGACAT TCAAACATCT CCTCCTCTGC CAATACAGAA ACATTAGATC AGCCAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007555322.1
CDS	283..1842
Translation	MLSVPTDLYVATKKPNAMDVFNFTSWNASEGNETEVMEERDSPYKTVEVVFIVLVAGSLSLVTIIGNILVMLSIKVNRNLQTVNNYFLFSLACADLIIGLCSMNLYTVYIVIGYWPLGPVVCDLWLALDYVVSNASVMNLLIISFDRYFCVTKPLSYPVKRTTKMAGMMIAAAWVLSFILWAPAILFWQFIVGGRTVPEKECYIQFFSNAAVTFGTAIAAFYLPVIIMIQLYWQISRASKSRVKKDNRKPSGSNPEPLLPGQRRESTPKPNNNNVPGEDGAHSQNQTADDGANQHDGKLQNGKGPSSSAADVETEGDSVAKENCAPAEEKESSNDSTSGSVAASNQKDEEQVASAANSSAEATQQLPRQRAKAGGSKLTCIKIKTKSPKGDYYTPSNATVEIVPTTERQNHVARKIVKMTKQPPNKKKKGAPSREKKVTRTIMAILVAFVATWTPYNVMVLINTFCSSCIPNTVWTIGYWLCYINSTINPACYALCNVTFKKTFKHLLLCQYRNIRSAR

Target ORF information:

RefSeq Version	XM_007555260.2
Organism	Poecilia formosa(Amazon molly)
Definition	Poecilia formosa muscarinic acetylcholine receptor M2 (LOC103140231), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007555260.2

ORF Insert Sequence:

ATGCTCTCTG TTCCAACCGA CTTGTACGTG GCAACAAAGA AACCAAATGC CATGGATGTC 
TTTAACTTTA CATCCTGGAA TGCCTCTGAA GGCAATGAGA CAGAAGTCAT GGAGGAGAGA 
GACAGTCCTT ACAAGACCGT GGAGGTAGTG TTCATCGTGC TGGTGGCTGG TTCCCTGAGT 
TTGGTCACTA TCATCGGCAA TATCCTGGTC ATGCTTTCCA TCAAGGTGAA CAGGAACCTA 
CAAACTGTCA ACAACTATTT TTTATTTAGC CTTGCATGTG CTGACCTAAT TATTGGACTG 
TGCTCCATGA ACTTGTACAC AGTATATATA GTAATAGGCT ACTGGCCCCT GGGGCCAGTC 
GTGTGCGACT TGTGGTTGGC TTTGGACTAT GTTGTCAGCA ATGCGTCTGT CATGAATCTC 
CTCATCATAA GCTTTGACAG ATACTTCTGT GTCACCAAGC CCCTGAGCTA CCCTGTAAAG 
AGAACCACCA AAATGGCAGG GATGATGATC GCTGCTGCCT GGGTCCTTTC CTTCATCCTC 
TGGGCACCTG CTATCCTCTT CTGGCAGTTC ATTGTTGGTG GGCGGACTGT GCCTGAGAAA 
GAGTGCTACA TTCAGTTCTT TTCCAATGCC GCTGTTACTT TTGGAACTGC CATTGCCGCC 
TTTTACCTGC CTGTCATTAT CATGATTCAG CTGTACTGGC AGATCTCGCG AGCGAGCAAA 
AGTCGAGTGA AGAAGGACAA CCGCAAGCCT TCAGGATCAA ACCCAGAACC TTTGTTACCG 
GGCCAGAGGA GAGAGAGCAC TCCGAAACCC AACAACAACA ATGTTCCTGG GGAAGACGGA 
GCGCATTCCC AGAACCAGAC TGCAGATGAT GGAGCCAACC AGCACGATGG AAAGCTGCAG 
AATGGAAAAG GGCCTTCATC GTCTGCTGCC GACGTAGAAA CGGAGGGTGA CAGTGTTGCA 
AAGGAGAACT GTGCTCCTGC AGAAGAGAAG GAGAGCTCAA ATGACTCCAC GTCCGGCAGT 
GTGGCAGCTT CCAATCAGAA GGACGAAGAA CAAGTCGCCT CTGCAGCAAA CTCCAGTGCT 
GAGGCCACGC AGCAGCTGCC ACGGCAACGA GCCAAGGCTG GGGGCTCCAA GCTGACCTGC 
ATCAAGATCA AGACCAAGTC CCCGAAGGGC GACTACTACA CACCGTCAAA CGCCACAGTT 
GAGATCGTGC CAACCACAGA ACGTCAGAAC CACGTGGCGA GGAAGATCGT GAAGATGACG 
AAGCAACCGC CAAACAAGAA GAAGAAAGGA GCCCCGTCGC GGGAGAAAAA AGTGACCCGC 
ACGATAATGG CCATCCTGGT GGCTTTCGTA GCTACCTGGA CTCCGTATAA CGTGATGGTG 
CTCATCAATA CTTTTTGTTC TAGTTGCATT CCCAACACTG TTTGGACTAT TGGTTACTGG 
CTGTGCTACA TCAACAGCAC CATCAACCCA GCCTGCTATG CTCTGTGTAA TGTCACGTTC 
AAAAAGACAT TCAAACATCT CCTCCTCTGC CAATACAGAA ACATTAGATC AGCCAGATAA