LOC110995412 cDNA ORF clone, Pieris rapae(cabbage white)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC110995412
Entrez Gene ID	110995412
Full Name	pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16
Gene Type	protein-coding
Organism	Pieris rapae(cabbage white)

mRNA and Protein(s)

mRNA	Protein	Name
XM_022262576.1	XP_022118268.1	pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16

Pathways from BioSystems
Gene Ontology

The following LOC110995412 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC110995412 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPi80223	XM_022262576.1 Latest version!	Pieris rapae pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 (LOC110995412), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC110995412 ( XM_022262576.1 ) cDNA ORF clone, Pieris rapae(cabbage white) -> XP_022118268.1 Pieris rapae pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 (LOC110995412), mRNA.

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CloneID	OPi80223
Clone ID Related Accession (Same CDS sequence)	XM_022262576.1
Accession Version	XM_022262576.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3558bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-08-09
Organism	Pieris rapae(cabbage white)
Product	pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019093323.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pieris rapae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGATA ATGATGAAAG TTTACACCGC TTGGAAGGGA CTTCAGGAGA TGCTCCTGGT 
GGGTTAATCA TTAGAAAGAA AGAAAAACCA GATGAGTTTC AGTTTGCTTT GCCCTCTCTC 
CTTGGCTTAG ATAAGCTAGC GGCAGCTAAA CGTAAGCAAA ACCGCTTAAT ATCTTTTCAA 
CCTGAGGACA CTGAATCAGA ATCAAATCAA GATGAAAGTG ACTCGTCTAC TGTAAAGGAA 
AGAAAATACA GGAGACATAA TGAAGAGACT CCAACCTACA CTGGTGGTCT TTCTGAAGAA 
GCTCGAAATA GAATGCTAGA ACGCTTAGAA AAAAAAACTA AAGAATCTAG AGAAAAAGGT 
GTACACAATT CTACACAAGA GGAAAAAAAA TCTAGATCAA AGGAGGAAGA TATAGATCGC 
CATTACCGTC GTTATGATCG TCGGGATAGG GACAGACGTT ATGATGATGA CAGAAGAGAC 
AGAGACTGGC AGAGACGAGA TAGAGGGTCT GATAGAAGAG ATAGAGATAG AAGAGAGAGA 
GATTCTGATA GAGACAGTTC CAGAAGAAGT TATTATGAAC CTAGGTTTAA GGATGAGCCC 
CGTACACCAA GTATTAAAGC ATTAAAACCA ACTGATAAGA CAGCCTGGGA TGAAGATGAT 
GATGACCCAA AAGCTGTTAG GAAGTCAAGT TGGGATTATC CAACACCCAA ACCAAGGGAA 
ACGCCAGATC GATCTACTAG GAGTGATAGA AGGCCTATCA GGGATTATAA AGGAAGACCA 
TATGATAATA CACCTAGACC CACACCTCAC AAATGGGTCA GTTCACGGCG AGGCCTCGAT 
GTAGATGATC CTGACTGGCA GGAAGCAGAA AAGAAATTAG ACCGAGCTTG GTACAATATG 
GGTGAAGGTG AGACTGATGA ATCTGATCCC TTTGCGGGAA CATCAGCTGA GTATATAGCT 
CGTAAGGAAG AACAAATAGA GAAACGCCGG AATCGGAAAG TTTCAGCTGC GAGGCAGCAA 
ATAGACAAGG ATAATGAGCT GTGGGAAAGG AACAGAATGC TTACAAGCGG AGTTGTTCAT 
TCTATAAATA TTAATAATGA TCTTGATGAG GAAAGTGTGG ACCGAGTACA TTTATTAGTA 
CACAATATTG TCCCTCCATT CCTGGATGGT CGTATTGTGT TTACAAAACA GCCGGAACCT 
GTTATACCTG TGAAAGATCC AACATCTGAT ATGGCGATTA ACGCGAGGAA AGGTTCTGCT 
CTAGTAAAAG CCTTTAGAGA ACAAAAGGAA AGACGAAAAG CTCAGAAGAA ACATTGGAAA 
TTGGAAGGAA CTAAAATAGG TAACATAATG GGTATTCAGA AGGAACAAGA AGAAATTGAA 
GATGGGCCCA CGAAGGAGGC CTATAAATAC GCCGAACATT TGGACAAGGA GGGTCAACAA 
CCAGAGTCGA AGTCAGATTT TGTGAAAAAG AAAACGATAT CAGAGCAAAG ACGGTTTTTG 
CCTGTTTTTG CCGTTAGAGA AGAATTAATG CAGGTCATAA GAGAGAATAA TGTAATTATC 
ATTGTTGGTG AAACGGGGAG TGGTAAAACG ACGCAGTTGA CCCAATACCT CCACGAGGAA 
GGGTTCAGTA AGATGGGCAT GATCGGGTGT ACGCAGCCGC GTCGTGTGGC CGCCATGTCC 
GTCGCCAAGA GGGTCGCTGA TGAAATGGGC ACGAGGCTCG GTGAGGAAGT AGGCTACGCC 
ATTCGTTTCG AAGACTGTAC GTCGCCCAAC ACGGTCATAA AGTATATGAC AGATGGTATT 
TTACTACGCG AGGGCTTACG GGAACCGGAT TTGGACAACT ACTGTGCGGT CATTATGGAC 
GAGGCCCACG AGAGGTCGCT GTCCACTGAT ATGCTGTTTG GATTGCTTCG GGAGGTGGTG 
GCACGCCGTT CTGATTTGAA GTTGATAGTG ACGTCAGCCA CAATGGACTC CAGCAAGTTC 
GCAACATTCT TCGGCAACGT ACCAACGTTC ACGATACCGG GACGTACTTT TCCTGTTGAG 
ACGTTTTTTG CTAAGAATGT CTGTGAAGAC TATGTGGATG GCGCTGTTAA GCAGGCTCTA 
CAAATCCACC TTCAGCCTGA TGAAGGTGAC ATCCTGATCT TCATGCCTGG TCAAGAGGAC 
ATAGAAGTTA CTTGCGAGGT ATTAACAGAG AGACTAGGAG ACTTAGATGC AGCCCCGCCC 
CTTACAGTGC TTCCGATATA CTCACAATTA CCAGCTGATT TGCAAGCGAA GATATTCCAA 
CGCGCACCGC CGGGACAAAG AAAATGTATA GTGGCTACAA ATATCGCTGA AACATCTCTA 
ACCGTGGATG GCATAATGTA TGTCATAGAC TGTGGTTACT GCAAGCTGAA GGTCTACAAT 
CCTAGAATTG GTATGGATGC ACTGCAGATA TATCCCGTAA GTCAAGCGAA CGCCAGACAG 
CGCGCTGGTC GCGCCGGTAG AACTGGGCCG GGAAAGGCAT TTTGCCTTTA CACGCAGAGG 
CAATTCCAAC ACGAGTTGTT ATCGGCAACT GTGCCCGAGA TACAGAGAAC GAATCTGGCC 
AATACTGTGC TGCTGTTGAA GTCTTTGGGT GTAGAAGATC TTCTGGCGTT TCACTTCATG 
GACCCACCAC CGCAGGACAC AATTCTTAAC TCAATGTACC AGCTGTGGAT TCTTGGAGCC 
CTGGACGGCA CGGGGGCCCT GACGCCGCTT GGCCGGCAGA TGGCGGAGTT CCCCTTGGAC 
CCGCCCCAGT GCCACATGCT TATCGTCTCC GCGGAAATGG GTTGCAGTGC TGAAGTACTT 
ATCATTGTAT CAATGCTGTC CGTCCCCACG GTGTTCTACC GACCCCAGGG GAGGGAGGAA 
GAATCCGACG CCGTGAAAGA GAAGTTCCAG GTGCCGGAGT CAGACCATCT CACTCTCTTG 
CATCTCTACA ACCAGTGGAA GAGTAATAAT TACTCAAGTT CGTGGTGTAC GGAGCATTTC 
GTCCACGCGA AAGCGATGCG CAAAGTGCGT GAGGTCCGAC AGCAGTTGAA GGACATCTTG 
CAGCAGCAGC GACTGCCGCT CCTCTCCTGT GGGACGGAAT GGGACACAGT GAGGAAGTGT 
ATTTGCTCCG CATACTTCCA ACAAGCGGCC CGTCTGAAGG GCATAGGAGA GTACGTGAAC 
TGTCGGACAG GCATGCCTTG CCACCTCCAC CCCACTTCGG CGCTCTTCGG TCTGGGCAAC 
TCGCCCGACT ACGTGGTATA CCACGAGCTG ATGATGACCA GCAAGGAGTA CATGCACTGC 
GTCACGGCCG TGGACGGAAG GTGGCTGGCG GAATTGGGAC CCATGTTCTT CTCGGTCAAG 
GAGACTGGAA AAACAAACCG CGACAAACGC AAAGAAGCAG CAGTCCACTT GCAGCGGATG 
GAAGAGGAGA TGAAAGAGGC CGAACATAAG ATGGCGGAAG AGAAGAAGAA GAAAGATCAA 
GACGTTCCTG TCAAACAGGA AGTTGCTACA CCTGGTTTAG CCACGCCCAA GAGGACGCCT 
CACAGACTTG GATTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022118268.1
CDS	1..3558
Translation	MADNDESLHRLEGTSGDAPGGLIIRKKEKPDEFQFALPSLLGLDKLAAAKRKQNRLISFQPEDTESESNQDESDSSTVKERKYRRHNEETPTYTGGLSEEARNRMLERLEKKTKESREKGVHNSTQEEKKSRSKEEDIDRHYRRYDRRDRDRRYDDDRRDRDWQRRDRGSDRRDRDRRERDSDRDSSRRSYYEPRFKDEPRTPSIKALKPTDKTAWDEDDDDPKAVRKSSWDYPTPKPRETPDRSTRSDRRPIRDYKGRPYDNTPRPTPHKWVSSRRGLDVDDPDWQEAEKKLDRAWYNMGEGETDESDPFAGTSAEYIARKEEQIEKRRNRKVSAARQQIDKDNELWERNRMLTSGVVHSININNDLDEESVDRVHLLVHNIVPPFLDGRIVFTKQPEPVIPVKDPTSDMAINARKGSALVKAFREQKERRKAQKKHWKLEGTKIGNIMGIQKEQEEIEDGPTKEAYKYAEHLDKEGQQPESKSDFVKKKTISEQRRFLPVFAVREELMQVIRENNVIIIVGETGSGKTTQLTQYLHEEGFSKMGMIGCTQPRRVAAMSVAKRVADEMGTRLGEEVGYAIRFEDCTSPNTVIKYMTDGILLREGLREPDLDNYCAVIMDEAHERSLSTDMLFGLLREVVARRSDLKLIVTSATMDSSKFATFFGNVPTFTIPGRTFPVETFFAKNVCEDYVDGAVKQALQIHLQPDEGDILIFMPGQEDIEVTCEVLTERLGDLDAAPPLTVLPIYSQLPADLQAKIFQRAPPGQRKCIVATNIAETSLTVDGIMYVIDCGYCKLKVYNPRIGMDALQIYPVSQANARQRAGRAGRTGPGKAFCLYTQRQFQHELLSATVPEIQRTNLANTVLLLKSLGVEDLLAFHFMDPPPQDTILNSMYQLWILGALDGTGALTPLGRQMAEFPLDPPQCHMLIVSAEMGCSAEVLIIVSMLSVPTVFYRPQGREEESDAVKEKFQVPESDHLTLLHLYNQWKSNNYSSSWCTEHFVHAKAMRKVREVRQQLKDILQQQRLPLLSCGTEWDTVRKCICSAYFQQAARLKGIGEYVNCRTGMPCHLHPTSALFGLGNSPDYVVYHELMMTSKEYMHCVTAVDGRWLAELGPMFFSVKETGKTNRDKRKEAAVHLQRMEEEMKEAEHKMAEEKKKKDQDVPVKQEVATPGLATPKRTPHRLGL

Target ORF information:

RefSeq Version	XM_022262576.1
Organism	Pieris rapae(cabbage white)
Definition	Pieris rapae pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 (LOC110995412), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022262576.1

ORF Insert Sequence:

ATGGCGGATA ATGATGAAAG TTTACACCGC TTGGAAGGGA CTTCAGGAGA TGCTCCTGGT 
GGGTTAATCA TTAGAAAGAA AGAAAAACCA GATGAGTTTC AGTTTGCTTT GCCCTCTCTC 
CTTGGCTTAG ATAAGCTAGC GGCAGCTAAA CGTAAGCAAA ACCGCTTAAT ATCTTTTCAA 
CCTGAGGACA CTGAATCAGA ATCAAATCAA GATGAAAGTG ACTCGTCTAC TGTAAAGGAA 
AGAAAATACA GGAGACATAA TGAAGAGACT CCAACCTACA CTGGTGGTCT TTCTGAAGAA 
GCTCGAAATA GAATGCTAGA ACGCTTAGAA AAAAAAACTA AAGAATCTAG AGAAAAAGGT 
GTACACAATT CTACACAAGA GGAAAAAAAA TCTAGATCAA AGGAGGAAGA TATAGATCGC 
CATTACCGTC GTTATGATCG TCGGGATAGG GACAGACGTT ATGATGATGA CAGAAGAGAC 
AGAGACTGGC AGAGACGAGA TAGAGGGTCT GATAGAAGAG ATAGAGATAG AAGAGAGAGA 
GATTCTGATA GAGACAGTTC CAGAAGAAGT TATTATGAAC CTAGGTTTAA GGATGAGCCC 
CGTACACCAA GTATTAAAGC ATTAAAACCA ACTGATAAGA CAGCCTGGGA TGAAGATGAT 
GATGACCCAA AAGCTGTTAG GAAGTCAAGT TGGGATTATC CAACACCCAA ACCAAGGGAA 
ACGCCAGATC GATCTACTAG GAGTGATAGA AGGCCTATCA GGGATTATAA AGGAAGACCA 
TATGATAATA CACCTAGACC CACACCTCAC AAATGGGTCA GTTCACGGCG AGGCCTCGAT 
GTAGATGATC CTGACTGGCA GGAAGCAGAA AAGAAATTAG ACCGAGCTTG GTACAATATG 
GGTGAAGGTG AGACTGATGA ATCTGATCCC TTTGCGGGAA CATCAGCTGA GTATATAGCT 
CGTAAGGAAG AACAAATAGA GAAACGCCGG AATCGGAAAG TTTCAGCTGC GAGGCAGCAA 
ATAGACAAGG ATAATGAGCT GTGGGAAAGG AACAGAATGC TTACAAGCGG AGTTGTTCAT 
TCTATAAATA TTAATAATGA TCTTGATGAG GAAAGTGTGG ACCGAGTACA TTTATTAGTA 
CACAATATTG TCCCTCCATT CCTGGATGGT CGTATTGTGT TTACAAAACA GCCGGAACCT 
GTTATACCTG TGAAAGATCC AACATCTGAT ATGGCGATTA ACGCGAGGAA AGGTTCTGCT 
CTAGTAAAAG CCTTTAGAGA ACAAAAGGAA AGACGAAAAG CTCAGAAGAA ACATTGGAAA 
TTGGAAGGAA CTAAAATAGG TAACATAATG GGTATTCAGA AGGAACAAGA AGAAATTGAA 
GATGGGCCCA CGAAGGAGGC CTATAAATAC GCCGAACATT TGGACAAGGA GGGTCAACAA 
CCAGAGTCGA AGTCAGATTT TGTGAAAAAG AAAACGATAT CAGAGCAAAG ACGGTTTTTG 
CCTGTTTTTG CCGTTAGAGA AGAATTAATG CAGGTCATAA GAGAGAATAA TGTAATTATC 
ATTGTTGGTG AAACGGGGAG TGGTAAAACG ACGCAGTTGA CCCAATACCT CCACGAGGAA 
GGGTTCAGTA AGATGGGCAT GATCGGGTGT ACGCAGCCGC GTCGTGTGGC CGCCATGTCC 
GTCGCCAAGA GGGTCGCTGA TGAAATGGGC ACGAGGCTCG GTGAGGAAGT AGGCTACGCC 
ATTCGTTTCG AAGACTGTAC GTCGCCCAAC ACGGTCATAA AGTATATGAC AGATGGTATT 
TTACTACGCG AGGGCTTACG GGAACCGGAT TTGGACAACT ACTGTGCGGT CATTATGGAC 
GAGGCCCACG AGAGGTCGCT GTCCACTGAT ATGCTGTTTG GATTGCTTCG GGAGGTGGTG 
GCACGCCGTT CTGATTTGAA GTTGATAGTG ACGTCAGCCA CAATGGACTC CAGCAAGTTC 
GCAACATTCT TCGGCAACGT ACCAACGTTC ACGATACCGG GACGTACTTT TCCTGTTGAG 
ACGTTTTTTG CTAAGAATGT CTGTGAAGAC TATGTGGATG GCGCTGTTAA GCAGGCTCTA 
CAAATCCACC TTCAGCCTGA TGAAGGTGAC ATCCTGATCT TCATGCCTGG TCAAGAGGAC 
ATAGAAGTTA CTTGCGAGGT ATTAACAGAG AGACTAGGAG ACTTAGATGC AGCCCCGCCC 
CTTACAGTGC TTCCGATATA CTCACAATTA CCAGCTGATT TGCAAGCGAA GATATTCCAA 
CGCGCACCGC CGGGACAAAG AAAATGTATA GTGGCTACAA ATATCGCTGA AACATCTCTA 
ACCGTGGATG GCATAATGTA TGTCATAGAC TGTGGTTACT GCAAGCTGAA GGTCTACAAT 
CCTAGAATTG GTATGGATGC ACTGCAGATA TATCCCGTAA GTCAAGCGAA CGCCAGACAG 
CGCGCTGGTC GCGCCGGTAG AACTGGGCCG GGAAAGGCAT TTTGCCTTTA CACGCAGAGG 
CAATTCCAAC ACGAGTTGTT ATCGGCAACT GTGCCCGAGA TACAGAGAAC GAATCTGGCC 
AATACTGTGC TGCTGTTGAA GTCTTTGGGT GTAGAAGATC TTCTGGCGTT TCACTTCATG 
GACCCACCAC CGCAGGACAC AATTCTTAAC TCAATGTACC AGCTGTGGAT TCTTGGAGCC 
CTGGACGGCA CGGGGGCCCT GACGCCGCTT GGCCGGCAGA TGGCGGAGTT CCCCTTGGAC 
CCGCCCCAGT GCCACATGCT TATCGTCTCC GCGGAAATGG GTTGCAGTGC TGAAGTACTT 
ATCATTGTAT CAATGCTGTC CGTCCCCACG GTGTTCTACC GACCCCAGGG GAGGGAGGAA 
GAATCCGACG CCGTGAAAGA GAAGTTCCAG GTGCCGGAGT CAGACCATCT CACTCTCTTG 
CATCTCTACA ACCAGTGGAA GAGTAATAAT TACTCAAGTT CGTGGTGTAC GGAGCATTTC 
GTCCACGCGA AAGCGATGCG CAAAGTGCGT GAGGTCCGAC AGCAGTTGAA GGACATCTTG 
CAGCAGCAGC GACTGCCGCT CCTCTCCTGT GGGACGGAAT GGGACACAGT GAGGAAGTGT 
ATTTGCTCCG CATACTTCCA ACAAGCGGCC CGTCTGAAGG GCATAGGAGA GTACGTGAAC 
TGTCGGACAG GCATGCCTTG CCACCTCCAC CCCACTTCGG CGCTCTTCGG TCTGGGCAAC 
TCGCCCGACT ACGTGGTATA CCACGAGCTG ATGATGACCA GCAAGGAGTA CATGCACTGC 
GTCACGGCCG TGGACGGAAG GTGGCTGGCG GAATTGGGAC CCATGTTCTT CTCGGTCAAG 
GAGACTGGAA AAACAAACCG CGACAAACGC AAAGAAGCAG CAGTCCACTT GCAGCGGATG 
GAAGAGGAGA TGAAAGAGGC CGAACATAAG ATGGCGGAAG AGAAGAAGAA GAAAGATCAA 
GACGTTCCTG TCAAACAGGA AGTTGCTACA CCTGGTTTAG CCACGCCCAA GAGGACGCCT 
CACAGACTTG GATTGTAG