BRIP1 cDNA ORF clone, Phascolarctos cinereus(koala)

Gene
Clones
gRNAs

Summary

Gene Symbol	BRIP1
Entrez Gene ID	110212039
Full Name	BRCA1 interacting protein C-terminal helicase 1
Gene Type	protein-coding
Organism	Phascolarctos cinereus(koala)

mRNA and Protein(s)

mRNA	Protein	Name
XM_020991818.1	XP_020847477.1	Fanconi anemia group J protein isoform X1
XM_020991819.1	XP_020847478.1	Fanconi anemia group J protein isoform X2

Pathways from BioSystems
Gene Ontology

The following BRIP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BRIP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPh258812	XM_020991818.1 Latest version!	Phascolarctos cinereus BRCA1 interacting protein C-terminal helicase 1 (BRIP1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OPh258813	XM_020991819.1 Latest version!	Phascolarctos cinereus BRCA1 interacting protein C-terminal helicase 1 (BRIP1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BRIP1 ( XM_020991818.1 ) cDNA ORF clone, Phascolarctos cinereus(koala) -> XP_020847477.1 Phascolarctos cinereus BRCA1 interacting protein C-terminal helicase 1 (BRIP1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPh258812
Clone ID Related Accession (Same CDS sequence)	XM_020991818.1
Accession Version	XM_020991818.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3699bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-09
Organism	Phascolarctos cinereus(koala)
Product	Fanconi anemia group J protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018343994.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Phascolarctos cinereus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTCAA CACTATCTGA ATACGTTATT GGTGGAGTGA AGATTTACTT CCCTTGTAAA 
GCTTACCCAT CACAGCTTGC TATGATGAAT TCTATTGTTA GAGGATTAAA CAGTAAGCAG 
CACTGTTTGT TGGAGAGCCC CACAGGAAGT GGGAAAAGCT TAGCCTTACT TTGTTCTGCC 
TTGGCATGGC AGCAGTCTCT CAGTGAAAAG CCAGGAGAGG AGAACTTGAA TAAAAAGACT 
GAAGTACCCT TGCCATGTTG TTGTATATGC CATTTGCACG ATCCCACTAA TGATATCAGT 
ATTAATGTGA ACCAAGGAGC TTCATGTTCT TTCACCAGTC CAATCGTGAC ATCTTCCATG 
AAAAATGAAA TCTCAACTTG CCAAGAAGAC TCTACTCCAG AAATCACACT TGCTGCGAAA 
TTATCTGCCA AGAAACAGGC ATCTTTGCTT AGCGATGGAA AGGATGATTT TCAAGTAGAC 
AAGAAAAGGA TTCGTCCCTT AGAAACCAAG CAGCAGGTTA GAAAGCGTCA TTGCTTTGCG 
AAGGATATAC AGAATTTAGA TATGAAAATT GCACCAGAAA GGATTGTGAA GCCAAACTCT 
CCACTGGGAA AAAAGATAAT GTCTTCTCCA CAAAAGCTTC CTGGTCCTTG TACTCGGTGT 
TCCTGTTCCA TTAAAAGTGG AAAAAGTCAA GATGACGATA ATATAACAAA GAAAGAACAT 
GGGGGAAAAT GCAAGACACC TAAAATATTT TTTGGGACTC GTACACATAA ACAGATAGCT 
CAGATTACTA GAGAACTGCG GAGGACAGCA TACTCTGGGG TCCGAATGAC TATTCTTTCT 
AGCAGGGATC ATACTTGTGT CCATCCTGAA GTAATCGGTA ATTTCAACAG GAATGAAAAA 
TGTATGGAGT TGATGGATGG AAAAAATGGT AGATCCTGTT ATTTCAACCA CGGTGTCCAT 
AAGATTAGTG AGCAACATGC TCTACAGACT TTCCCAGGGA TGGGCAGGGC CTGGGATATA 
GAAGATCTTG TGAGCCTGGG GAGAAAGTTA AAGGCATGTC CATATTATGC AGCTCGAGAG 
CTAATGTTAG ATGCTGACAT TGTGTTCTGT CCCTACAACT ATCTCCTGGA TTCACAGATA 
AGGGAAAGTA TGGATATCAA TTTGAAAGAG CAGATCATCA TTTTAGATGA AGCCCATAAT 
ATTGAGGACT GTGCACGTGA GTCAGCCAGT TACAGTGTTA CAGAAATCCA ACTTAGGTTT 
GCTCGAGATG AACTAGATGT CATGGTCAGC AACAATATAA GGAAGAAAAA CCATGAACCC 
TTGCGAGCTG TTTGCTATAG CCTCATAAAT TGGTTACAGG AAAACTCTAA ACATCTTGTA 
GAAAGACATT ATGAATCATC CTGTAAAGTG TGGAGTGGAA GTGAAATGAT CTCTCACTTC 
TATAAAATGG GCATAACTGC TGCTACCTTT CCAATATTAC AGGGACATTT TACTGCTGTC 
CTTCAAAAAG AAGAAAAAAT TATGACCATT AATGGTAAAG AGCAAATCAG AGAAATACCT 
GTTGTTAGTC CCTCGACTCA GATTATGCTC AAAGGACTGT TTATGGTACT GGATTATCTT 
TTTAGGCAGA AGAGCAGGTT TGCAGATGAT TACAAAGTCG CTTTACAGCA AACTTACTCT 
TGGACAAGCC AGATTGATAT TCCAGACAAA AATGGTTTCA TTGCTTTACC TAAAAATAAG 
AAACATTCTA GACAAAAAAT GGCAGTAAAT GTACTAAACT TTTGGTGCTT GAATCCAGCT 
GTGGCCTTTT CGGATATTAG TAGTAATGTA CGGACGATTG TTCTGTCATC TGGTACTCTT 
TCACCAATGA AATCTTTTTC ATCTGAACTT GGTGTGACAT TTACTATCCA ACTAGAGGCT 
AATCATGTCA TTCAGAACTC ACAGGTTTGG GTTGGCACTA TTGGGTCAGG CCCCAAGGGC 
CGTAATCTCT GTGCCACCTT CCAGCACGCA GAGACCTTTG CATTCCAGGA TGAAGTTGGA 
GCACTTCTCT TGTCCGTATG CCAAACAGTG AGCCAAGGGA TTTTGTGCTT CTTGCCATCT 
TACAAGTTAT TGGATAAATT AAAAGAACGT TGGATACACA CTGGTTTATG GCATAATCTT 
GAGCTAGTGA AGACAGTCAT CACGGAACCA CAATGGGGAC AGAAAGCTGC ATTTGATGAA 
GTGTTACAAT TGTACTACAA TTCGATCAAG TGTAAAGGAG ACCGAGATGG TGCCCTCCTT 
GTGGCAGTTT GCCGAGGTAA AGTGAGTGAA GGTTTGGATT TCTCAGATGA AAATGCCCGT 
GCTGTCATAA CAATAGGAAT TCCATTTCCA AATGTGAATA ATCTTCAGGT TGAATTAAAG 
AGAAAGTACA ACGACCAGCA TTCAAAACTA AGAGGTCTTT TACCTGGACG CCAGTGGTAT 
GAAATTCAAG CTTATAGGGC GCTGAACCAG GCACTCGGCA GGTGTATTCG GCATAAAAAT 
GATTGGGGAG CTCTTATTTT AGTTGATGAT CGCTTCAGGA GAAACCCAGA TCAATATATA 
TCTGGACTTT CCAAATGGAT TCGGCAACAG ATTAAGCACC ATGAAACTTT TGAGAGTGCA 
TTGGAGTCCT TGGCTGCATT TTCTAAAAAG CATCAAAAAG TCATTGATGT GTCATCCCCA 
AACAGTAGCG AGTCTGAACA AGAAGTGGCC TGTTTAAAGG ATGAGTCCTC GACATCTTTA 
TTAGAATCAA CAATTCACCC ATCACCAAAT GTTCTTGGAG AAGATGAACG GAAACCTTTT 
GTCCAGGAGC TTCAGACTTC TACAATGATG GCTGTGGAGC TCAAACTACA CAGTCACCCA 
AGTAACATCA TCACAGCCCA GAATAAAAAT GATTCATTAT TGTTGGAATA TTCTCCACAG 
AAAATAAAGA GGGAAAAATG TTTAATATCA AGATCCACAA GTCCAACCTT CAGTAAACAA 
ATAGAAAGGA ATAGCTGTCC TGAATCTGAC TCTGTGAAGA GATATTTTAC CCCTAAGTTG 
CTGACTTCCA TACCTGTGTG CACATTTTCA GAGAACCATG ACATCAGTCC TGTCCCTTCC 
AAAGAGGAAA AGAGGGAAAG TACAGTCGGT CTGTCTCTTG CTAATAGAGG CCAGTCCTCG 
TCCCTGTCAG GAAACATACC ATTTGGCCCA GGTCCACAGT CAGAAAGCAT TACTTCATTG 
ACCAATACTG TTAGTAAAAA AGATCCTGCT GAACAGCTAC TCTACCCTGA AGAAGTCCTG 
GATCCAGACT TCGATCTTAG TCCTGTACAT GGAGAAGCTG AATTTTCCAA TGTTAATGTA 
GATATTGAAA CAACAGAAGA AGACGAATCT GTCTATTTCA CACCGGAACT TTATGATGAT 
ACAGAATCAG ACGTAGAAGA AAATGAAGAG CTACTTGAAA CTGGTGGAAT AAATCAGAGG 
ACTGAAAATT CAGATTACAT TATAGCAGAA AACCTTTTGG AAATTGGTTG TACCAAAGGA 
ATGGGTTCAG ATCACACAGA CATGAAGTTG AATAGAATCA TGCAAAATGA AGAACATAAA 
GTTGACGACA TTAGTAATAA TGTAAAGGCA GCAAATATAC ATGAACAGGA GCTGGAAAGA 
ACTCAGGAAA TGGACACCAT AGGTAACATC AGCACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020847477.1
CDS	254..3952
Translation	MSSTLSEYVIGGVKIYFPCKAYPSQLAMMNSIVRGLNSKQHCLLESPTGSGKSLALLCSALAWQQSLSEKPGEENLNKKTEVPLPCCCICHLHDPTNDISINVNQGASCSFTSPIVTSSMKNEISTCQEDSTPEITLAAKLSAKKQASLLSDGKDDFQVDKKRIRPLETKQQVRKRHCFAKDIQNLDMKIAPERIVKPNSPLGKKIMSSPQKLPGPCTRCSCSIKSGKSQDDDNITKKEHGGKCKTPKIFFGTRTHKQIAQITRELRRTAYSGVRMTILSSRDHTCVHPEVIGNFNRNEKCMELMDGKNGRSCYFNHGVHKISEQHALQTFPGMGRAWDIEDLVSLGRKLKACPYYAARELMLDADIVFCPYNYLLDSQIRESMDINLKEQIIILDEAHNIEDCARESASYSVTEIQLRFARDELDVMVSNNIRKKNHEPLRAVCYSLINWLQENSKHLVERHYESSCKVWSGSEMISHFYKMGITAATFPILQGHFTAVLQKEEKIMTINGKEQIREIPVVSPSTQIMLKGLFMVLDYLFRQKSRFADDYKVALQQTYSWTSQIDIPDKNGFIALPKNKKHSRQKMAVNVLNFWCLNPAVAFSDISSNVRTIVLSSGTLSPMKSFSSELGVTFTIQLEANHVIQNSQVWVGTIGSGPKGRNLCATFQHAETFAFQDEVGALLLSVCQTVSQGILCFLPSYKLLDKLKERWIHTGLWHNLELVKTVITEPQWGQKAAFDEVLQLYYNSIKCKGDRDGALLVAVCRGKVSEGLDFSDENARAVITIGIPFPNVNNLQVELKRKYNDQHSKLRGLLPGRQWYEIQAYRALNQALGRCIRHKNDWGALILVDDRFRRNPDQYISGLSKWIRQQIKHHETFESALESLAAFSKKHQKVIDVSSPNSSESEQEVACLKDESSTSLLESTIHPSPNVLGEDERKPFVQELQTSTMMAVELKLHSHPSNIITAQNKNDSLLLEYSPQKIKREKCLISRSTSPTFSKQIERNSCPESDSVKRYFTPKLLTSIPVCTFSENHDISPVPSKEEKRESTVGLSLANRGQSSSLSGNIPFGPGPQSESITSLTNTVSKKDPAEQLLYPEEVLDPDFDLSPVHGEAEFSNVNVDIETTEEDESVYFTPELYDDTESDVEENEELLETGGINQRTENSDYIIAENLLEIGCTKGMGSDHTDMKLNRIMQNEEHKVDDISNNVKAANIHEQELERTQEMDTIGNIST

Target ORF information:

RefSeq Version	XM_020991818.1
Organism	Phascolarctos cinereus(koala)
Definition	Phascolarctos cinereus BRCA1 interacting protein C-terminal helicase 1 (BRIP1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020991818.1

ORF Insert Sequence:

ATGTCTTCAA CACTATCTGA ATACGTTATT GGTGGAGTGA AGATTTACTT CCCTTGTAAA 
GCTTACCCAT CACAGCTTGC TATGATGAAT TCTATTGTTA GAGGATTAAA CAGTAAGCAG 
CACTGTTTGT TGGAGAGCCC CACAGGAAGT GGGAAAAGCT TAGCCTTACT TTGTTCTGCC 
TTGGCATGGC AGCAGTCTCT CAGTGAAAAG CCAGGAGAGG AGAACTTGAA TAAAAAGACT 
GAAGTACCCT TGCCATGTTG TTGTATATGC CATTTGCACG ATCCCACTAA TGATATCAGT 
ATTAATGTGA ACCAAGGAGC TTCATGTTCT TTCACCAGTC CAATCGTGAC ATCTTCCATG 
AAAAATGAAA TCTCAACTTG CCAAGAAGAC TCTACTCCAG AAATCACACT TGCTGCGAAA 
TTATCTGCCA AGAAACAGGC ATCTTTGCTT AGCGATGGAA AGGATGATTT TCAAGTAGAC 
AAGAAAAGGA TTCGTCCCTT AGAAACCAAG CAGCAGGTTA GAAAGCGTCA TTGCTTTGCG 
AAGGATATAC AGAATTTAGA TATGAAAATT GCACCAGAAA GGATTGTGAA GCCAAACTCT 
CCACTGGGAA AAAAGATAAT GTCTTCTCCA CAAAAGCTTC CTGGTCCTTG TACTCGGTGT 
TCCTGTTCCA TTAAAAGTGG AAAAAGTCAA GATGACGATA ATATAACAAA GAAAGAACAT 
GGGGGAAAAT GCAAGACACC TAAAATATTT TTTGGGACTC GTACACATAA ACAGATAGCT 
CAGATTACTA GAGAACTGCG GAGGACAGCA TACTCTGGGG TCCGAATGAC TATTCTTTCT 
AGCAGGGATC ATACTTGTGT CCATCCTGAA GTAATCGGTA ATTTCAACAG GAATGAAAAA 
TGTATGGAGT TGATGGATGG AAAAAATGGT AGATCCTGTT ATTTCAACCA CGGTGTCCAT 
AAGATTAGTG AGCAACATGC TCTACAGACT TTCCCAGGGA TGGGCAGGGC CTGGGATATA 
GAAGATCTTG TGAGCCTGGG GAGAAAGTTA AAGGCATGTC CATATTATGC AGCTCGAGAG 
CTAATGTTAG ATGCTGACAT TGTGTTCTGT CCCTACAACT ATCTCCTGGA TTCACAGATA 
AGGGAAAGTA TGGATATCAA TTTGAAAGAG CAGATCATCA TTTTAGATGA AGCCCATAAT 
ATTGAGGACT GTGCACGTGA GTCAGCCAGT TACAGTGTTA CAGAAATCCA ACTTAGGTTT 
GCTCGAGATG AACTAGATGT CATGGTCAGC AACAATATAA GGAAGAAAAA CCATGAACCC 
TTGCGAGCTG TTTGCTATAG CCTCATAAAT TGGTTACAGG AAAACTCTAA ACATCTTGTA 
GAAAGACATT ATGAATCATC CTGTAAAGTG TGGAGTGGAA GTGAAATGAT CTCTCACTTC 
TATAAAATGG GCATAACTGC TGCTACCTTT CCAATATTAC AGGGACATTT TACTGCTGTC 
CTTCAAAAAG AAGAAAAAAT TATGACCATT AATGGTAAAG AGCAAATCAG AGAAATACCT 
GTTGTTAGTC CCTCGACTCA GATTATGCTC AAAGGACTGT TTATGGTACT GGATTATCTT 
TTTAGGCAGA AGAGCAGGTT TGCAGATGAT TACAAAGTCG CTTTACAGCA AACTTACTCT 
TGGACAAGCC AGATTGATAT TCCAGACAAA AATGGTTTCA TTGCTTTACC TAAAAATAAG 
AAACATTCTA GACAAAAAAT GGCAGTAAAT GTACTAAACT TTTGGTGCTT GAATCCAGCT 
GTGGCCTTTT CGGATATTAG TAGTAATGTA CGGACGATTG TTCTGTCATC TGGTACTCTT 
TCACCAATGA AATCTTTTTC ATCTGAACTT GGTGTGACAT TTACTATCCA ACTAGAGGCT 
AATCATGTCA TTCAGAACTC ACAGGTTTGG GTTGGCACTA TTGGGTCAGG CCCCAAGGGC 
CGTAATCTCT GTGCCACCTT CCAGCACGCA GAGACCTTTG CATTCCAGGA TGAAGTTGGA 
GCACTTCTCT TGTCCGTATG CCAAACAGTG AGCCAAGGGA TTTTGTGCTT CTTGCCATCT 
TACAAGTTAT TGGATAAATT AAAAGAACGT TGGATACACA CTGGTTTATG GCATAATCTT 
GAGCTAGTGA AGACAGTCAT CACGGAACCA CAATGGGGAC AGAAAGCTGC ATTTGATGAA 
GTGTTACAAT TGTACTACAA TTCGATCAAG TGTAAAGGAG ACCGAGATGG TGCCCTCCTT 
GTGGCAGTTT GCCGAGGTAA AGTGAGTGAA GGTTTGGATT TCTCAGATGA AAATGCCCGT 
GCTGTCATAA CAATAGGAAT TCCATTTCCA AATGTGAATA ATCTTCAGGT TGAATTAAAG 
AGAAAGTACA ACGACCAGCA TTCAAAACTA AGAGGTCTTT TACCTGGACG CCAGTGGTAT 
GAAATTCAAG CTTATAGGGC GCTGAACCAG GCACTCGGCA GGTGTATTCG GCATAAAAAT 
GATTGGGGAG CTCTTATTTT AGTTGATGAT CGCTTCAGGA GAAACCCAGA TCAATATATA 
TCTGGACTTT CCAAATGGAT TCGGCAACAG ATTAAGCACC ATGAAACTTT TGAGAGTGCA 
TTGGAGTCCT TGGCTGCATT TTCTAAAAAG CATCAAAAAG TCATTGATGT GTCATCCCCA 
AACAGTAGCG AGTCTGAACA AGAAGTGGCC TGTTTAAAGG ATGAGTCCTC GACATCTTTA 
TTAGAATCAA CAATTCACCC ATCACCAAAT GTTCTTGGAG AAGATGAACG GAAACCTTTT 
GTCCAGGAGC TTCAGACTTC TACAATGATG GCTGTGGAGC TCAAACTACA CAGTCACCCA 
AGTAACATCA TCACAGCCCA GAATAAAAAT GATTCATTAT TGTTGGAATA TTCTCCACAG 
AAAATAAAGA GGGAAAAATG TTTAATATCA AGATCCACAA GTCCAACCTT CAGTAAACAA 
ATAGAAAGGA ATAGCTGTCC TGAATCTGAC TCTGTGAAGA GATATTTTAC CCCTAAGTTG 
CTGACTTCCA TACCTGTGTG CACATTTTCA GAGAACCATG ACATCAGTCC TGTCCCTTCC 
AAAGAGGAAA AGAGGGAAAG TACAGTCGGT CTGTCTCTTG CTAATAGAGG CCAGTCCTCG 
TCCCTGTCAG GAAACATACC ATTTGGCCCA GGTCCACAGT CAGAAAGCAT TACTTCATTG 
ACCAATACTG TTAGTAAAAA AGATCCTGCT GAACAGCTAC TCTACCCTGA AGAAGTCCTG 
GATCCAGACT TCGATCTTAG TCCTGTACAT GGAGAAGCTG AATTTTCCAA TGTTAATGTA 
GATATTGAAA CAACAGAAGA AGACGAATCT GTCTATTTCA CACCGGAACT TTATGATGAT 
ACAGAATCAG ACGTAGAAGA AAATGAAGAG CTACTTGAAA CTGGTGGAAT AAATCAGAGG 
ACTGAAAATT CAGATTACAT TATAGCAGAA AACCTTTTGG AAATTGGTTG TACCAAAGGA 
ATGGGTTCAG ATCACACAGA CATGAAGTTG AATAGAATCA TGCAAAATGA AGAACATAAA 
GTTGACGACA TTAGTAATAA TGTAAAGGCA GCAAATATAC ATGAACAGGA GCTGGAAAGA 
ACTCAGGAAA TGGACACCAT AGGTAACATC AGCACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BRIP1 ( XM_020991819.1 ) cDNA ORF clone, Phascolarctos cinereus(koala) -> XP_020847478.1 Phascolarctos cinereus BRCA1 interacting protein C-terminal helicase 1 (BRIP1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPh258813
Clone ID Related Accession (Same CDS sequence)	XM_020991819.1
Accession Version	XM_020991819.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3696bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-09
Organism	Phascolarctos cinereus(koala)
Product	Fanconi anemia group J protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018343994.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Phascolarctos cinereus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTCAA CACTATCTGA ATACGTTATT GGTGGAGTGA AGATTTACTT CCCTTGTAAA 
GCTTACCCAT CACAGCTTGC TATGATGAAT TCTATTGTTA GAGGATTAAA CAGTAAGCAG 
CACTGTTTGT TGGAGAGCCC CACAGGAAGT GGGAAAAGCT TAGCCTTACT TTGTTCTGCC 
TTGGCATGGC AGCAGTCTCT CAGTGAAAAG CCAGGAGAGG AGAACTTGAA TAAAAAGACT 
GAAGTACCCT TGCCATGTTG TTGTATATGC CATTTGCACG ATCCCACTAA TGATATCAGT 
ATTAATGTGA ACCAAGGAGC TTCATGTTCT TTCACCAGTC CAATCGTGAC ATCTTCCATG 
AAAAATGAAA TCTCAACTTG CCAAGACTCT ACTCCAGAAA TCACACTTGC TGCGAAATTA 
TCTGCCAAGA AACAGGCATC TTTGCTTAGC GATGGAAAGG ATGATTTTCA AGTAGACAAG 
AAAAGGATTC GTCCCTTAGA AACCAAGCAG CAGGTTAGAA AGCGTCATTG CTTTGCGAAG 
GATATACAGA ATTTAGATAT GAAAATTGCA CCAGAAAGGA TTGTGAAGCC AAACTCTCCA 
CTGGGAAAAA AGATAATGTC TTCTCCACAA AAGCTTCCTG GTCCTTGTAC TCGGTGTTCC 
TGTTCCATTA AAAGTGGAAA AAGTCAAGAT GACGATAATA TAACAAAGAA AGAACATGGG 
GGAAAATGCA AGACACCTAA AATATTTTTT GGGACTCGTA CACATAAACA GATAGCTCAG 
ATTACTAGAG AACTGCGGAG GACAGCATAC TCTGGGGTCC GAATGACTAT TCTTTCTAGC 
AGGGATCATA CTTGTGTCCA TCCTGAAGTA ATCGGTAATT TCAACAGGAA TGAAAAATGT 
ATGGAGTTGA TGGATGGAAA AAATGGTAGA TCCTGTTATT TCAACCACGG TGTCCATAAG 
ATTAGTGAGC AACATGCTCT ACAGACTTTC CCAGGGATGG GCAGGGCCTG GGATATAGAA 
GATCTTGTGA GCCTGGGGAG AAAGTTAAAG GCATGTCCAT ATTATGCAGC TCGAGAGCTA 
ATGTTAGATG CTGACATTGT GTTCTGTCCC TACAACTATC TCCTGGATTC ACAGATAAGG 
GAAAGTATGG ATATCAATTT GAAAGAGCAG ATCATCATTT TAGATGAAGC CCATAATATT 
GAGGACTGTG CACGTGAGTC AGCCAGTTAC AGTGTTACAG AAATCCAACT TAGGTTTGCT 
CGAGATGAAC TAGATGTCAT GGTCAGCAAC AATATAAGGA AGAAAAACCA TGAACCCTTG 
CGAGCTGTTT GCTATAGCCT CATAAATTGG TTACAGGAAA ACTCTAAACA TCTTGTAGAA 
AGACATTATG AATCATCCTG TAAAGTGTGG AGTGGAAGTG AAATGATCTC TCACTTCTAT 
AAAATGGGCA TAACTGCTGC TACCTTTCCA ATATTACAGG GACATTTTAC TGCTGTCCTT 
CAAAAAGAAG AAAAAATTAT GACCATTAAT GGTAAAGAGC AAATCAGAGA AATACCTGTT 
GTTAGTCCCT CGACTCAGAT TATGCTCAAA GGACTGTTTA TGGTACTGGA TTATCTTTTT 
AGGCAGAAGA GCAGGTTTGC AGATGATTAC AAAGTCGCTT TACAGCAAAC TTACTCTTGG 
ACAAGCCAGA TTGATATTCC AGACAAAAAT GGTTTCATTG CTTTACCTAA AAATAAGAAA 
CATTCTAGAC AAAAAATGGC AGTAAATGTA CTAAACTTTT GGTGCTTGAA TCCAGCTGTG 
GCCTTTTCGG ATATTAGTAG TAATGTACGG ACGATTGTTC TGTCATCTGG TACTCTTTCA 
CCAATGAAAT CTTTTTCATC TGAACTTGGT GTGACATTTA CTATCCAACT AGAGGCTAAT 
CATGTCATTC AGAACTCACA GGTTTGGGTT GGCACTATTG GGTCAGGCCC CAAGGGCCGT 
AATCTCTGTG CCACCTTCCA GCACGCAGAG ACCTTTGCAT TCCAGGATGA AGTTGGAGCA 
CTTCTCTTGT CCGTATGCCA AACAGTGAGC CAAGGGATTT TGTGCTTCTT GCCATCTTAC 
AAGTTATTGG ATAAATTAAA AGAACGTTGG ATACACACTG GTTTATGGCA TAATCTTGAG 
CTAGTGAAGA CAGTCATCAC GGAACCACAA TGGGGACAGA AAGCTGCATT TGATGAAGTG 
TTACAATTGT ACTACAATTC GATCAAGTGT AAAGGAGACC GAGATGGTGC CCTCCTTGTG 
GCAGTTTGCC GAGGTAAAGT GAGTGAAGGT TTGGATTTCT CAGATGAAAA TGCCCGTGCT 
GTCATAACAA TAGGAATTCC ATTTCCAAAT GTGAATAATC TTCAGGTTGA ATTAAAGAGA 
AAGTACAACG ACCAGCATTC AAAACTAAGA GGTCTTTTAC CTGGACGCCA GTGGTATGAA 
ATTCAAGCTT ATAGGGCGCT GAACCAGGCA CTCGGCAGGT GTATTCGGCA TAAAAATGAT 
TGGGGAGCTC TTATTTTAGT TGATGATCGC TTCAGGAGAA ACCCAGATCA ATATATATCT 
GGACTTTCCA AATGGATTCG GCAACAGATT AAGCACCATG AAACTTTTGA GAGTGCATTG 
GAGTCCTTGG CTGCATTTTC TAAAAAGCAT CAAAAAGTCA TTGATGTGTC ATCCCCAAAC 
AGTAGCGAGT CTGAACAAGA AGTGGCCTGT TTAAAGGATG AGTCCTCGAC ATCTTTATTA 
GAATCAACAA TTCACCCATC ACCAAATGTT CTTGGAGAAG ATGAACGGAA ACCTTTTGTC 
CAGGAGCTTC AGACTTCTAC AATGATGGCT GTGGAGCTCA AACTACACAG TCACCCAAGT 
AACATCATCA CAGCCCAGAA TAAAAATGAT TCATTATTGT TGGAATATTC TCCACAGAAA 
ATAAAGAGGG AAAAATGTTT AATATCAAGA TCCACAAGTC CAACCTTCAG TAAACAAATA 
GAAAGGAATA GCTGTCCTGA ATCTGACTCT GTGAAGAGAT ATTTTACCCC TAAGTTGCTG 
ACTTCCATAC CTGTGTGCAC ATTTTCAGAG AACCATGACA TCAGTCCTGT CCCTTCCAAA 
GAGGAAAAGA GGGAAAGTAC AGTCGGTCTG TCTCTTGCTA ATAGAGGCCA GTCCTCGTCC 
CTGTCAGGAA ACATACCATT TGGCCCAGGT CCACAGTCAG AAAGCATTAC TTCATTGACC 
AATACTGTTA GTAAAAAAGA TCCTGCTGAA CAGCTACTCT ACCCTGAAGA AGTCCTGGAT 
CCAGACTTCG ATCTTAGTCC TGTACATGGA GAAGCTGAAT TTTCCAATGT TAATGTAGAT 
ATTGAAACAA CAGAAGAAGA CGAATCTGTC TATTTCACAC CGGAACTTTA TGATGATACA 
GAATCAGACG TAGAAGAAAA TGAAGAGCTA CTTGAAACTG GTGGAATAAA TCAGAGGACT 
GAAAATTCAG ATTACATTAT AGCAGAAAAC CTTTTGGAAA TTGGTTGTAC CAAAGGAATG 
GGTTCAGATC ACACAGACAT GAAGTTGAAT AGAATCATGC AAAATGAAGA ACATAAAGTT 
GACGACATTA GTAATAATGT AAAGGCAGCA AATATACATG AACAGGAGCT GGAAAGAACT 
CAGGAAATGG ACACCATAGG TAACATCAGC ACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020847478.1
CDS	255..3950
Translation	MSSTLSEYVIGGVKIYFPCKAYPSQLAMMNSIVRGLNSKQHCLLESPTGSGKSLALLCSALAWQQSLSEKPGEENLNKKTEVPLPCCCICHLHDPTNDISINVNQGASCSFTSPIVTSSMKNEISTCQDSTPEITLAAKLSAKKQASLLSDGKDDFQVDKKRIRPLETKQQVRKRHCFAKDIQNLDMKIAPERIVKPNSPLGKKIMSSPQKLPGPCTRCSCSIKSGKSQDDDNITKKEHGGKCKTPKIFFGTRTHKQIAQITRELRRTAYSGVRMTILSSRDHTCVHPEVIGNFNRNEKCMELMDGKNGRSCYFNHGVHKISEQHALQTFPGMGRAWDIEDLVSLGRKLKACPYYAARELMLDADIVFCPYNYLLDSQIRESMDINLKEQIIILDEAHNIEDCARESASYSVTEIQLRFARDELDVMVSNNIRKKNHEPLRAVCYSLINWLQENSKHLVERHYESSCKVWSGSEMISHFYKMGITAATFPILQGHFTAVLQKEEKIMTINGKEQIREIPVVSPSTQIMLKGLFMVLDYLFRQKSRFADDYKVALQQTYSWTSQIDIPDKNGFIALPKNKKHSRQKMAVNVLNFWCLNPAVAFSDISSNVRTIVLSSGTLSPMKSFSSELGVTFTIQLEANHVIQNSQVWVGTIGSGPKGRNLCATFQHAETFAFQDEVGALLLSVCQTVSQGILCFLPSYKLLDKLKERWIHTGLWHNLELVKTVITEPQWGQKAAFDEVLQLYYNSIKCKGDRDGALLVAVCRGKVSEGLDFSDENARAVITIGIPFPNVNNLQVELKRKYNDQHSKLRGLLPGRQWYEIQAYRALNQALGRCIRHKNDWGALILVDDRFRRNPDQYISGLSKWIRQQIKHHETFESALESLAAFSKKHQKVIDVSSPNSSESEQEVACLKDESSTSLLESTIHPSPNVLGEDERKPFVQELQTSTMMAVELKLHSHPSNIITAQNKNDSLLLEYSPQKIKREKCLISRSTSPTFSKQIERNSCPESDSVKRYFTPKLLTSIPVCTFSENHDISPVPSKEEKRESTVGLSLANRGQSSSLSGNIPFGPGPQSESITSLTNTVSKKDPAEQLLYPEEVLDPDFDLSPVHGEAEFSNVNVDIETTEEDESVYFTPELYDDTESDVEENEELLETGGINQRTENSDYIIAENLLEIGCTKGMGSDHTDMKLNRIMQNEEHKVDDISNNVKAANIHEQELERTQEMDTIGNIST

Target ORF information:

RefSeq Version	XM_020991819.1
Organism	Phascolarctos cinereus(koala)
Definition	Phascolarctos cinereus BRCA1 interacting protein C-terminal helicase 1 (BRIP1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020991819.1

ORF Insert Sequence:

ATGTCTTCAA CACTATCTGA ATACGTTATT GGTGGAGTGA AGATTTACTT CCCTTGTAAA 
GCTTACCCAT CACAGCTTGC TATGATGAAT TCTATTGTTA GAGGATTAAA CAGTAAGCAG 
CACTGTTTGT TGGAGAGCCC CACAGGAAGT GGGAAAAGCT TAGCCTTACT TTGTTCTGCC 
TTGGCATGGC AGCAGTCTCT CAGTGAAAAG CCAGGAGAGG AGAACTTGAA TAAAAAGACT 
GAAGTACCCT TGCCATGTTG TTGTATATGC CATTTGCACG ATCCCACTAA TGATATCAGT 
ATTAATGTGA ACCAAGGAGC TTCATGTTCT TTCACCAGTC CAATCGTGAC ATCTTCCATG 
AAAAATGAAA TCTCAACTTG CCAAGACTCT ACTCCAGAAA TCACACTTGC TGCGAAATTA 
TCTGCCAAGA AACAGGCATC TTTGCTTAGC GATGGAAAGG ATGATTTTCA AGTAGACAAG 
AAAAGGATTC GTCCCTTAGA AACCAAGCAG CAGGTTAGAA AGCGTCATTG CTTTGCGAAG 
GATATACAGA ATTTAGATAT GAAAATTGCA CCAGAAAGGA TTGTGAAGCC AAACTCTCCA 
CTGGGAAAAA AGATAATGTC TTCTCCACAA AAGCTTCCTG GTCCTTGTAC TCGGTGTTCC 
TGTTCCATTA AAAGTGGAAA AAGTCAAGAT GACGATAATA TAACAAAGAA AGAACATGGG 
GGAAAATGCA AGACACCTAA AATATTTTTT GGGACTCGTA CACATAAACA GATAGCTCAG 
ATTACTAGAG AACTGCGGAG GACAGCATAC TCTGGGGTCC GAATGACTAT TCTTTCTAGC 
AGGGATCATA CTTGTGTCCA TCCTGAAGTA ATCGGTAATT TCAACAGGAA TGAAAAATGT 
ATGGAGTTGA TGGATGGAAA AAATGGTAGA TCCTGTTATT TCAACCACGG TGTCCATAAG 
ATTAGTGAGC AACATGCTCT ACAGACTTTC CCAGGGATGG GCAGGGCCTG GGATATAGAA 
GATCTTGTGA GCCTGGGGAG AAAGTTAAAG GCATGTCCAT ATTATGCAGC TCGAGAGCTA 
ATGTTAGATG CTGACATTGT GTTCTGTCCC TACAACTATC TCCTGGATTC ACAGATAAGG 
GAAAGTATGG ATATCAATTT GAAAGAGCAG ATCATCATTT TAGATGAAGC CCATAATATT 
GAGGACTGTG CACGTGAGTC AGCCAGTTAC AGTGTTACAG AAATCCAACT TAGGTTTGCT 
CGAGATGAAC TAGATGTCAT GGTCAGCAAC AATATAAGGA AGAAAAACCA TGAACCCTTG 
CGAGCTGTTT GCTATAGCCT CATAAATTGG TTACAGGAAA ACTCTAAACA TCTTGTAGAA 
AGACATTATG AATCATCCTG TAAAGTGTGG AGTGGAAGTG AAATGATCTC TCACTTCTAT 
AAAATGGGCA TAACTGCTGC TACCTTTCCA ATATTACAGG GACATTTTAC TGCTGTCCTT 
CAAAAAGAAG AAAAAATTAT GACCATTAAT GGTAAAGAGC AAATCAGAGA AATACCTGTT 
GTTAGTCCCT CGACTCAGAT TATGCTCAAA GGACTGTTTA TGGTACTGGA TTATCTTTTT 
AGGCAGAAGA GCAGGTTTGC AGATGATTAC AAAGTCGCTT TACAGCAAAC TTACTCTTGG 
ACAAGCCAGA TTGATATTCC AGACAAAAAT GGTTTCATTG CTTTACCTAA AAATAAGAAA 
CATTCTAGAC AAAAAATGGC AGTAAATGTA CTAAACTTTT GGTGCTTGAA TCCAGCTGTG 
GCCTTTTCGG ATATTAGTAG TAATGTACGG ACGATTGTTC TGTCATCTGG TACTCTTTCA 
CCAATGAAAT CTTTTTCATC TGAACTTGGT GTGACATTTA CTATCCAACT AGAGGCTAAT 
CATGTCATTC AGAACTCACA GGTTTGGGTT GGCACTATTG GGTCAGGCCC CAAGGGCCGT 
AATCTCTGTG CCACCTTCCA GCACGCAGAG ACCTTTGCAT TCCAGGATGA AGTTGGAGCA 
CTTCTCTTGT CCGTATGCCA AACAGTGAGC CAAGGGATTT TGTGCTTCTT GCCATCTTAC 
AAGTTATTGG ATAAATTAAA AGAACGTTGG ATACACACTG GTTTATGGCA TAATCTTGAG 
CTAGTGAAGA CAGTCATCAC GGAACCACAA TGGGGACAGA AAGCTGCATT TGATGAAGTG 
TTACAATTGT ACTACAATTC GATCAAGTGT AAAGGAGACC GAGATGGTGC CCTCCTTGTG 
GCAGTTTGCC GAGGTAAAGT GAGTGAAGGT TTGGATTTCT CAGATGAAAA TGCCCGTGCT 
GTCATAACAA TAGGAATTCC ATTTCCAAAT GTGAATAATC TTCAGGTTGA ATTAAAGAGA 
AAGTACAACG ACCAGCATTC AAAACTAAGA GGTCTTTTAC CTGGACGCCA GTGGTATGAA 
ATTCAAGCTT ATAGGGCGCT GAACCAGGCA CTCGGCAGGT GTATTCGGCA TAAAAATGAT 
TGGGGAGCTC TTATTTTAGT TGATGATCGC TTCAGGAGAA ACCCAGATCA ATATATATCT 
GGACTTTCCA AATGGATTCG GCAACAGATT AAGCACCATG AAACTTTTGA GAGTGCATTG 
GAGTCCTTGG CTGCATTTTC TAAAAAGCAT CAAAAAGTCA TTGATGTGTC ATCCCCAAAC 
AGTAGCGAGT CTGAACAAGA AGTGGCCTGT TTAAAGGATG AGTCCTCGAC ATCTTTATTA 
GAATCAACAA TTCACCCATC ACCAAATGTT CTTGGAGAAG ATGAACGGAA ACCTTTTGTC 
CAGGAGCTTC AGACTTCTAC AATGATGGCT GTGGAGCTCA AACTACACAG TCACCCAAGT 
AACATCATCA CAGCCCAGAA TAAAAATGAT TCATTATTGT TGGAATATTC TCCACAGAAA 
ATAAAGAGGG AAAAATGTTT AATATCAAGA TCCACAAGTC CAACCTTCAG TAAACAAATA 
GAAAGGAATA GCTGTCCTGA ATCTGACTCT GTGAAGAGAT ATTTTACCCC TAAGTTGCTG 
ACTTCCATAC CTGTGTGCAC ATTTTCAGAG AACCATGACA TCAGTCCTGT CCCTTCCAAA 
GAGGAAAAGA GGGAAAGTAC AGTCGGTCTG TCTCTTGCTA ATAGAGGCCA GTCCTCGTCC 
CTGTCAGGAA ACATACCATT TGGCCCAGGT CCACAGTCAG AAAGCATTAC TTCATTGACC 
AATACTGTTA GTAAAAAAGA TCCTGCTGAA CAGCTACTCT ACCCTGAAGA AGTCCTGGAT 
CCAGACTTCG ATCTTAGTCC TGTACATGGA GAAGCTGAAT TTTCCAATGT TAATGTAGAT 
ATTGAAACAA CAGAAGAAGA CGAATCTGTC TATTTCACAC CGGAACTTTA TGATGATACA 
GAATCAGACG TAGAAGAAAA TGAAGAGCTA CTTGAAACTG GTGGAATAAA TCAGAGGACT 
GAAAATTCAG ATTACATTAT AGCAGAAAAC CTTTTGGAAA TTGGTTGTAC CAAAGGAATG 
GGTTCAGATC ACACAGACAT GAAGTTGAAT AGAATCATGC AAAATGAAGA ACATAAAGTT 
GACGACATTA GTAATAATGT AAAGGCAGCA AATATACATG AACAGGAGCT GGAAAGAACT 
CAGGAAATGG ACACCATAGG TAACATCAGC ACATGA