HECW1 cDNA ORF clone, Pelodiscus sinensis(Chinese soft-shelled turtle)

Gene
Clones
gRNAs

Summary

Gene Symbol	HECW1
Entrez Gene ID	102460922
Full Name	HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1
Gene Type	protein-coding
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014574083.1	XP_014429569.1	E3 ubiquitin-protein ligase HECW1 isoform X1
XM_025185175.1	XP_025040960.1	E3 ubiquitin-protein ligase HECW1 isoform X2
XM_006124096.3	XP_006124158.1	E3 ubiquitin-protein ligase HECW1 isoform X3
XM_025185176.1	XP_025040961.1	E3 ubiquitin-protein ligase HECW1 isoform X4
XM_006124096.2	XP_006124158.1	E3 ubiquitin-protein ligase HECW1 isoform X2
XM_025185174.1	XP_025040959.1	E3 ubiquitin-protein ligase HECW1 isoform X1
XM_014574083.2	XP_014429569.1	E3 ubiquitin-protein ligase HECW1 isoform X2

Pathways from BioSystems
Gene Ontology

The following HECW1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HECW1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPe151171	XM_025185176.1 Latest version!	Pelodiscus sinensis HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OPe151170	XM_025185174.1 Latest version!	Pelodiscus sinensis HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OPe30299	XM_014574083.2 Latest version!	Pelodiscus sinensis HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OPe30299	XM_025185175.1 Latest version!	Pelodiscus sinensis HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OPe30300	XM_006124096.3 Latest version!	Pelodiscus sinensis HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
View Old Accession Versions >>
OPe30300	XM_006124096.2	Pelodiscus sinensis HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OPe30299	XM_014574083.1	Pelodiscus sinensis HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HECW1 ( XM_025185176.1 ) cDNA ORF clone, Pelodiscus sinensis(Chinese soft-shelled turtle) -> XP_025040961.1 Pelodiscus sinensis HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OPe151171
Clone ID Related Accession (Same CDS sequence)	XM_025185176.1
Accession Version	XM_025185176.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3591bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-03
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Product	E3 ubiquitin-protein ligase HECW1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005855477.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pelodiscus sinensis Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGATC TAAAACCTGC CCTGGCTCCT GCTCGGGGGA GCATGATCTG TGCCCGCACC 
GGCATCCTCC AGAATCTGTA CCAAAACAGG TTCCTAGGCC TGGCTGCCAT GGCGTCTCCA 
TCTAGGAATT CACAGAACCG GCGCCGGTGT AAAGAACCTC TCAGATACAG CTATAACCCT 
GACCAGTTTA ATAACATGGA CCTCAGGAAC AATGCTCATG AGACAGTAAC CATTCCACGA 
TCCACCAGTG ATACTGACCT GGTCACTTCA GACAGCCGGT CTACTCTCAT GGTCAGCAGC 
TCATATTACT CCATTGGACA TTCACAAGAC CTAGTTATCT ATTGGGATAT AAAGGAAGAA 
GTGGATGCAG GGGACTGGAT CGGCATGTAT CTCATTGATG AAGTCTTGTC TGAAAACTTT 
CTGGACTACA AAAACCGTGG GGTCAATGGC TCTCATCGTG GTCAGATTAT CTGGAAGATT 
GATGCCAACT CTTACTTCGT AGAGTCTGAA ACAAAGATCT GCTTCAAATA CTACCATGGA 
GTGAGTGGAG CACTACGAGC TACGACCCCC AGTGTCACAG TGAAAAACAG TTCTGCTCCC 
ATTTTTAAAA GCATCGTCAA TGAAGATCCA GTCCAGGGTC AAGGAAGTCG GAGACTGATC 
AGTTTTTCAC TGTCAGATTT CCAGGCTTTG GGTCTGAAAA AAGGAATGTT CTTTAACCCA 
GACCCATACC TGAAGATTTC CATTCAGCCT GGAAAGCACA GCATCTTCCC AGCACTTCCT 
CACCATGGAC AAGAGAAGAG ATCCAAGATC ACATGTAATA CTGTGAACCC TATCTGGCAA 
GGAGAGCACT TCAGCTTTGT TTCCCTTCCC ACGGATGTTC TGGAAATAGA AGTTAAGGAC 
AAGTTTGCAA AGAGCCGGCC AATCATTAAA CGCTTCTTGG GGAAACTTTC CATGCCAGTG 
CAACGCCTGC TGGAGAGACA CGCAATAGGG GACAGGGTTG TCAGCTACAC TCTGGGACGT 
AGGCTTCCAA CAGATCATGT CAGCGGCCAG CTGCAGTTTC GGTTTGAGAT AACTTCTTCT 
ATCCATCCAG ATGATGAAGA GGTCTCCATT AGTGCAGATC CTGAGTCAGC TGAACTGCAG 
GAAAGCCCTG TGAACTGCAC CGCAGAGGAA ATCCTGGGTG ATTGTCCTTT ACTCCATAAT 
TCTCAGCCAG TCAGCCAGCT TCCTTCCCTG AGGCCTGACC ATCATCACTA TCCAACTATT 
GATGAGCCTC TTCCACCAAA CTGGGAGGCT CGGATAGACA GCCATGGAAG GGTATTTTAT 
GTGGATCACG TGAATCGAAC CACAACATGG CAGCGTCCTA CAGCAGCAGC CACTCCAGAT 
GGAATACGCA GATCTGGTTC AATCCAACAA ATGGAGCAGC TCAATCGGCG GTATCAAAAT 
ATTCAGCGGA CTATTGCAAC AGAGAGGCCA GAGGATGATG CTGTTGTGAA CAACAGAGTT 
GAGAGGCTCT CCACTGGAGG AGGAAGTGAC TCTGAGACAG ACTCTTCCCA GCCAAACTCA 
GAAATTAGGC GAGAAGGGTC CCTTTCTCCA GTGAATTCTC AAAAAATCAC CTTGCTGCTA 
CAGTCTCCAG CTGTAAAGTT TATCACAAAC CCTGAATTCT TCACCGTGCT GCATGCAAAC 
TATAGTGCCT ATCGAGTCTT CACCAACAGC ACCTGCTTGA AGCACATGAT TCTAAAGATC 
CGCAGAGATG CTCGGAATTT TGAGCGCTAT CAGCACAATA GGGACCTGGT AAATTTTATC 
AATATGTTTG CTGACACCCG ACTGGAACTT CCACGGGGCT GGGAGATCAA AACAGACCAA 
CAGGGCAAGT CCTTCTTTGT GGACCACAAC AGCCGTGCTA CCACTTTTAT CGATCCTAGG 
ATCCCACTTC AAAATGGCCG TCTCCCTAAT CATCTGACTC ACAGGCAGCA TCTCCAGAGA 
CTTCGCAGCT ACAGTGCTGG AGAGGCATCA GAAGTCTCCC GGAATAGAGG CATATCTCTG 
TTGACCAGAC CAGGCAACAG CCTAGTAACA GCTATTCGAA ACCAGCACCA TCATGAGTCA 
TTACCTCTAG CTTACAATGA CAAGATTGTC GCATTTCTAC GCCAACCAAA CATTTTTGAG 
ATGCTGCAAG AGCGTCAGCC AAGCCTGGCC AGGAACCATG CACTCAGGGA GAAGATCCAT 
TACATTCGGA CAGAGGGTAC ACATGGGCTT GAAAAGCTGT CCTGTGATGC AGATTTAGTA 
ATTCTATTGA GCCTCTTTGA GGAGGATATT ATGTCCTACA TACCCCTGCA GGCTGCCTTC 
CACCCTGGCT ATAGCTTCTC TCCTCGCTGT TCACCCTGCT CCTCACCTCA GAATTCTCCA 
GGATTACAGC GAGCTAGTGC ACGAGCGCCC TCTCCATATA GGAGAGACTT TGAAGCCAAG 
CTACGCAATT TCTATCGAAA ACTTGAAGCC AAAGGATATG GACAAGGTCC AGGTAAAATT 
AAGTTAATTA TACGGCGTGA CCACTTGTTG GAAGGCACCT TCAATCAGGT GATGGCTTAT 
TCACGCAAGG AGCTTCAGCG GAACAAACTC TATGTCACAT TCGTTGGAGA GGAAGGCTTG 
GACTACAGCG GTCCCTCCCG AGAGTTCTTC TTCCTTCTGT CTCAGGAGCT CTTCAATCCT 
TACTATGGCT TGTTTGAGTA TTCAGCCAAC GACACTTACA CTGTACAGAT CAGCCCCATG 
TCTGCGTTTG TGGAAAACCA CCTTGAATGG TTCAGGTTCA GCGGTCGTAT CCTTGGCCTT 
GCTCTCATTC ATCAGTACCT TCTTGATGCT TTCTTCACAA GGCCGTTCTA TAAAGCACTA 
CTGAGGCTGC CGTGTGATTT AAGTGACCTG GAGTACCTGG ATGAGGAGTT TCACCAGAGT 
TTACAGTGGA TGAAGGATAA CAACATCACT GATATCCTGG ATCTCACATT CACCGTGAAC 
GAGGAGGTGT TTGGACAGGT GACAGAAAGG GAGCTGAAAT CAGGGGGTTC AAATACACAG 
GTGACAGAAA AGAACAAGAA GGAGTACATT GAGAGAATGG TGAAGTGGCG TGTGGAACGA 
GGAGTGGTTC AGCAAACAGA GGCTCTAGTC CGTGGCTTCT ATGAAGTTGT AGACTCCAGG 
CTTGTCTCCA TTTTTGATGC CAGAGAGCTA GAGTTGGTGA TTGCAGGTAC AGCAGAAATA 
GACCTCAATG ACTGGCGTAA CAACACAGAA TATCGTGGAG GTTACCATGA TGGGCATATT 
GTCATACGGT GGTTCTGGGC AGCAGTGGAG AGATTTAACA ATGAACAGAG ACTACGGTTA 
CTGCAGTTTG TCACTGGAAC ATCTAGTGTA CCATATGAGG GTTTTGCAGC CCTTCGAGGG 
AGCAATGGGT TACGGCGTTT CTGTATAGAA AAATGGGGGA AAATAACATC TCTTCCTAGG 
GCGCACACAT GTTTCAACCG CTTGGATCTC CCGCCCTACC CATCTTATTC TATGCTGTAT 
GAAAAGCTGT TGACAGCTGT TGAAGAAACT AGCACCTTTG GACTTGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025040961.1
CDS	44..3634
Translation	MWDLKPALAPARGSMICARTGILQNLYQNRFLGLAAMASPSRNSQNRRRCKEPLRYSYNPDQFNNMDLRNNAHETVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIYWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDANSYFVESETKICFKYYHGVSGALRATTPSVTVKNSSAPIFKSIVNEDPVQGQGSRRLISFSLSDFQALGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQEKRSKITCNTVNPIWQGEHFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPDDEEVSISADPESAELQESPVNCTAEEILGDCPLLHNSQPVSQLPSLRPDHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGIRRSGSIQQMEQLNRRYQNIQRTIATERPEDDAVVNNRVERLSTGGGSDSETDSSQPNSEIRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTNSTCLKHMILKIRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGISLLTRPGNSLVTAIRNQHHHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHALREKIHYIRTEGTHGLEKLSCDADLVILLSLFEEDIMSYIPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGYGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGSNTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSIFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_025185176.1
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Definition	Pelodiscus sinensis HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025185176.1

ORF Insert Sequence:

ATGTGGGATC TAAAACCTGC CCTGGCTCCT GCTCGGGGGA GCATGATCTG TGCCCGCACC 
GGCATCCTCC AGAATCTGTA CCAAAACAGG TTCCTAGGCC TGGCTGCCAT GGCGTCTCCA 
TCTAGGAATT CACAGAACCG GCGCCGGTGT AAAGAACCTC TCAGATACAG CTATAACCCT 
GACCAGTTTA ATAACATGGA CCTCAGGAAC AATGCTCATG AGACAGTAAC CATTCCACGA 
TCCACCAGTG ATACTGACCT GGTCACTTCA GACAGCCGGT CTACTCTCAT GGTCAGCAGC 
TCATATTACT CCATTGGACA TTCACAAGAC CTAGTTATCT ATTGGGATAT AAAGGAAGAA 
GTGGATGCAG GGGACTGGAT CGGCATGTAT CTCATTGATG AAGTCTTGTC TGAAAACTTT 
CTGGACTACA AAAACCGTGG GGTCAATGGC TCTCATCGTG GTCAGATTAT CTGGAAGATT 
GATGCCAACT CTTACTTCGT AGAGTCTGAA ACAAAGATCT GCTTCAAATA CTACCATGGA 
GTGAGTGGAG CACTACGAGC TACGACCCCC AGTGTCACAG TGAAAAACAG TTCTGCTCCC 
ATTTTTAAAA GCATCGTCAA TGAAGATCCA GTCCAGGGTC AAGGAAGTCG GAGACTGATC 
AGTTTTTCAC TGTCAGATTT CCAGGCTTTG GGTCTGAAAA AAGGAATGTT CTTTAACCCA 
GACCCATACC TGAAGATTTC CATTCAGCCT GGAAAGCACA GCATCTTCCC AGCACTTCCT 
CACCATGGAC AAGAGAAGAG ATCCAAGATC ACATGTAATA CTGTGAACCC TATCTGGCAA 
GGAGAGCACT TCAGCTTTGT TTCCCTTCCC ACGGATGTTC TGGAAATAGA AGTTAAGGAC 
AAGTTTGCAA AGAGCCGGCC AATCATTAAA CGCTTCTTGG GGAAACTTTC CATGCCAGTG 
CAACGCCTGC TGGAGAGACA CGCAATAGGG GACAGGGTTG TCAGCTACAC TCTGGGACGT 
AGGCTTCCAA CAGATCATGT CAGCGGCCAG CTGCAGTTTC GGTTTGAGAT AACTTCTTCT 
ATCCATCCAG ATGATGAAGA GGTCTCCATT AGTGCAGATC CTGAGTCAGC TGAACTGCAG 
GAAAGCCCTG TGAACTGCAC CGCAGAGGAA ATCCTGGGTG ATTGTCCTTT ACTCCATAAT 
TCTCAGCCAG TCAGCCAGCT TCCTTCCCTG AGGCCTGACC ATCATCACTA TCCAACTATT 
GATGAGCCTC TTCCACCAAA CTGGGAGGCT CGGATAGACA GCCATGGAAG GGTATTTTAT 
GTGGATCACG TGAATCGAAC CACAACATGG CAGCGTCCTA CAGCAGCAGC CACTCCAGAT 
GGAATACGCA GATCTGGTTC AATCCAACAA ATGGAGCAGC TCAATCGGCG GTATCAAAAT 
ATTCAGCGGA CTATTGCAAC AGAGAGGCCA GAGGATGATG CTGTTGTGAA CAACAGAGTT 
GAGAGGCTCT CCACTGGAGG AGGAAGTGAC TCTGAGACAG ACTCTTCCCA GCCAAACTCA 
GAAATTAGGC GAGAAGGGTC CCTTTCTCCA GTGAATTCTC AAAAAATCAC CTTGCTGCTA 
CAGTCTCCAG CTGTAAAGTT TATCACAAAC CCTGAATTCT TCACCGTGCT GCATGCAAAC 
TATAGTGCCT ATCGAGTCTT CACCAACAGC ACCTGCTTGA AGCACATGAT TCTAAAGATC 
CGCAGAGATG CTCGGAATTT TGAGCGCTAT CAGCACAATA GGGACCTGGT AAATTTTATC 
AATATGTTTG CTGACACCCG ACTGGAACTT CCACGGGGCT GGGAGATCAA AACAGACCAA 
CAGGGCAAGT CCTTCTTTGT GGACCACAAC AGCCGTGCTA CCACTTTTAT CGATCCTAGG 
ATCCCACTTC AAAATGGCCG TCTCCCTAAT CATCTGACTC ACAGGCAGCA TCTCCAGAGA 
CTTCGCAGCT ACAGTGCTGG AGAGGCATCA GAAGTCTCCC GGAATAGAGG CATATCTCTG 
TTGACCAGAC CAGGCAACAG CCTAGTAACA GCTATTCGAA ACCAGCACCA TCATGAGTCA 
TTACCTCTAG CTTACAATGA CAAGATTGTC GCATTTCTAC GCCAACCAAA CATTTTTGAG 
ATGCTGCAAG AGCGTCAGCC AAGCCTGGCC AGGAACCATG CACTCAGGGA GAAGATCCAT 
TACATTCGGA CAGAGGGTAC ACATGGGCTT GAAAAGCTGT CCTGTGATGC AGATTTAGTA 
ATTCTATTGA GCCTCTTTGA GGAGGATATT ATGTCCTACA TACCCCTGCA GGCTGCCTTC 
CACCCTGGCT ATAGCTTCTC TCCTCGCTGT TCACCCTGCT CCTCACCTCA GAATTCTCCA 
GGATTACAGC GAGCTAGTGC ACGAGCGCCC TCTCCATATA GGAGAGACTT TGAAGCCAAG 
CTACGCAATT TCTATCGAAA ACTTGAAGCC AAAGGATATG GACAAGGTCC AGGTAAAATT 
AAGTTAATTA TACGGCGTGA CCACTTGTTG GAAGGCACCT TCAATCAGGT GATGGCTTAT 
TCACGCAAGG AGCTTCAGCG GAACAAACTC TATGTCACAT TCGTTGGAGA GGAAGGCTTG 
GACTACAGCG GTCCCTCCCG AGAGTTCTTC TTCCTTCTGT CTCAGGAGCT CTTCAATCCT 
TACTATGGCT TGTTTGAGTA TTCAGCCAAC GACACTTACA CTGTACAGAT CAGCCCCATG 
TCTGCGTTTG TGGAAAACCA CCTTGAATGG TTCAGGTTCA GCGGTCGTAT CCTTGGCCTT 
GCTCTCATTC ATCAGTACCT TCTTGATGCT TTCTTCACAA GGCCGTTCTA TAAAGCACTA 
CTGAGGCTGC CGTGTGATTT AAGTGACCTG GAGTACCTGG ATGAGGAGTT TCACCAGAGT 
TTACAGTGGA TGAAGGATAA CAACATCACT GATATCCTGG ATCTCACATT CACCGTGAAC 
GAGGAGGTGT TTGGACAGGT GACAGAAAGG GAGCTGAAAT CAGGGGGTTC AAATACACAG 
GTGACAGAAA AGAACAAGAA GGAGTACATT GAGAGAATGG TGAAGTGGCG TGTGGAACGA 
GGAGTGGTTC AGCAAACAGA GGCTCTAGTC CGTGGCTTCT ATGAAGTTGT AGACTCCAGG 
CTTGTCTCCA TTTTTGATGC CAGAGAGCTA GAGTTGGTGA TTGCAGGTAC AGCAGAAATA 
GACCTCAATG ACTGGCGTAA CAACACAGAA TATCGTGGAG GTTACCATGA TGGGCATATT 
GTCATACGGT GGTTCTGGGC AGCAGTGGAG AGATTTAACA ATGAACAGAG ACTACGGTTA 
CTGCAGTTTG TCACTGGAAC ATCTAGTGTA CCATATGAGG GTTTTGCAGC CCTTCGAGGG 
AGCAATGGGT TACGGCGTTT CTGTATAGAA AAATGGGGGA AAATAACATC TCTTCCTAGG 
GCGCACACAT GTTTCAACCG CTTGGATCTC CCGCCCTACC CATCTTATTC TATGCTGTAT 
GAAAAGCTGT TGACAGCTGT TGAAGAAACT AGCACCTTTG GACTTGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_025185174.1 ) cDNA ORF clone, Pelodiscus sinensis(Chinese soft-shelled turtle) -> XP_025040959.1 Pelodiscus sinensis HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPe151170
Clone ID Related Accession (Same CDS sequence)	XM_025185174.1
Accession Version	XM_025185174.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4848bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-03
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Product	E3 ubiquitin-protein ligase HECW1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005855477.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pelodiscus sinensis Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGATC TAAAACCTGC CCTGGCTCCT GCTCGGGGGA GCATGATCTG TGCCCGCACC 
GGCATCCTCC AGAATCTGTA CCAAAACAGG TTCCTAGGCC TGGCTGCCAT GGCGTCTCCA 
TCTAGGAATT CACAGAACCG GCGCCGGTGT AAAGAACCTC TCAGATACAG CTATAACCCT 
GACCAGTTTA ATAACATGGA CCTCAGGAAC AATGCTCATG AGACAGTAAC CATTCCACGA 
TCCACCAGTG ATACTGACCT GGTCACTTCA GACAGCCGGT CTACTCTCAT GGTCAGCAGC 
TCATATTACT CCATTGGACA TTCACAAGAC CTAGTTATCT ATTGGGATAT AAAGGAAGAA 
GTGGATGCAG GGGACTGGAT CGGCATGTAT CTCATTGATG AAGTCTTGTC TGAAAACTTT 
CTGGACTACA AAAACCGTGG GGTCAATGGC TCTCATCGTG GTCAGATTAT CTGGAAGATT 
GATGCCAACT CTTACTTCGT AGAGTCTGAA ACAAAGATCT GCTTCAAATA CTACCATGGA 
GTGAGTGGAG CACTACGAGC TACGACCCCC AGTGTCACAG TGAAAAACAG TTCTGCTCCC 
ATTTTTAAAA GCATCGTCAA TGAAGATCCA GTCCAGGGTC AAGGAAGTCG GAGACTGATC 
AGTTTTTCAC TGTCAGATTT CCAGGCTTTG GGTCTGAAAA AAGGAATGTT CTTTAACCCA 
GACCCATACC TGAAGATTTC CATTCAGCCT GGAAAGCACA GCATCTTCCC AGCACTTCCT 
CACCATGGAC AAGAGAAGAG ATCCAAGATC ACATGTAATA CTGTGAACCC TATCTGGCAA 
GGAGAGCACT TCAGCTTTGT TTCCCTTCCC ACGGATGTTC TGGAAATAGA AGTTAAGGAC 
AAGTTTGCAA AGAGCCGGCC AATCATTAAA CGCTTCTTGG GGAAACTTTC CATGCCAGTG 
CAACGCCTGC TGGAGAGACA CGCAATAGGG GACAGGGTTG TCAGCTACAC TCTGGGACGT 
AGGCTTCCAA CAGATCATGT CAGCGGCCAG CTGCAGTTTC GGTTTGAGAT AACTTCTTCT 
ATCCATCCAG ATGATGAAGA GGTCTCCATT AGTGCAGATC CTGAGTCAGC TGAACTGCAG 
GAAAGCCCTG TGAACTGCAC CGCAGAGGAA ATCCTGGGTG AGCCTCCCTG CACCGGCACA 
GTGACCTCAC AAAGTACATC TCCAGAGCAG CTAAATGGAT GTCATGTGAA AAGTGTCAAT 
GTTGATATCC CTGGGGAAGT CAAACAGAAC AGCTTAAATG AAGAGCTAGC AGGAAATAGG 
GAGGTTGGTG TAGTGCCAGC AGATGCTACT GCGTCCTTGG AATCCATCCC TGGGGAAGTG 
CTGCCTTCCC TGAGTGCTCT GGAACTCTCT GAGCAGCTGG CTCTGCTGGT TTGCACACCT 
CCTCCAGAGA TTGACTTTAG GGAAAATAAT AAAGTCAATT CTGTTACTCA GGGACACGAG 
GACATCTTCA CCCGCATGGA AGCACTAGAT ACCGATGAGG TGAGTGCAGA CATACAGACT 
GTCAAGTACT TGGAGAAAAT TCAGGATGAG GAAGGCGCTT CAGCCCAGGA AGAGGAAGAC 
AACAAGCTGC AGCTAAGAAT GTCAGGGAAA AGGAAAAACA GGCCCTGCTC CTTGCCTGTG 
TCTGAGCTGG AGACGGTGAT TGCATCAGCC TGTGGTGAGC CTGAGACCCC TCGAACGCAC 
TACATTCGGA TCCACAATCT GCTTCACAGC TTGCCCTCAG CACAGATGAG TAGTGATGGA 
GAGGAGGAGC CGGGAGGCGG CGAGAATGAC GAAGAGTCAA CGGTCAAGGA AGTGTCAGAG 
AAGGATGTGG TTAGCGAGTC TGAGACGCTA GCGGCAGATC CTCCCCCTGA AAGCGGAACT 
GATGAGTATT TCAGCCGGGG GACTGTGCCC CGCAGCACCT CAGTTGAACA CCTCTCAGAC 
TTAAATGGGC AGCTGTTGGA TGGAGAACAT CCAACGGCTG GAAGTGAGAG TGAATCAAGT 
TCCAGGCCAT ACGGGGACAA TGAGTGTGAC ACCTCTTGCT ACAGCCCATC CTGCTACAGC 
ACCTCCTGCT ACAGTACGTC CTGCTACAGC ACCTCCTGCT ATAGCCCCTC CTGTTATGAC 
AGTAACAATC GCTTTTCCAG CCATACCCGT TTCTCCTCTG TTGACAGTGC AAAGATTTCT 
GAGAGCACAG TCTTTTCCTC ACAGGATGAT GAGGAAGAGG AAAACAGTGC CTTTGAGTCA 
GTGCCAGATT CAGTGCAAAG CCCTGAACTG GACAGGGAAC AAGTGGATGT CTCCGCCCAG 
TGGCCAGATG AACTCATAGC TCATGGTGGG AATTCACAAA GAGCAACAGA GATCCTAGAA 
TCCCCAGTAG CAGGTCCAAG CAACAGAAGA GAAGGTGATT GTCCTTTACT CCATAATTCT 
CAGCCAGTCA GCCAGCTTCC TTCCCTGAGG CCTGACCATC ATCACTATCC AACTATTGAT 
GAGCCTCTTC CACCAAACTG GGAGGCTCGG ATAGACAGCC ATGGAAGGGT ATTTTATGTG 
GATCACGTGA ATCGAACCAC AACATGGCAG CGTCCTACAG CAGCAGCCAC TCCAGATGGA 
ATACGCAGAT CTGGTTCAAT CCAACAAATG GAGCAGCTCA ATCGGCGGTA TCAAAATATT 
CAGCGGACTA TTGCAACAGA GAGGCCAGAG GATGATGCTG TTGTGAACAA CAGAGTTGAG 
AGGCTCTCCA CTGGAGGAGG AAGTGACTCT GAGACAGACT CTTCCCAGCC AAACTCAGAA 
ATTAGGCGAG AAGGGTCCCT TTCTCCAGTG AATTCTCAAA AAATCACCTT GCTGCTACAG 
TCTCCAGCTG TAAAGTTTAT CACAAACCCT GAATTCTTCA CCGTGCTGCA TGCAAACTAT 
AGTGCCTATC GAGTCTTCAC CAACAGCACC TGCTTGAAGC ACATGATTCT AAAGATCCGC 
AGAGATGCTC GGAATTTTGA GCGCTATCAG CACAATAGGG ACCTGGTAAA TTTTATCAAT 
ATGTTTGCTG ACACCCGACT GGAACTTCCA CGGGGCTGGG AGATCAAAAC AGACCAACAG 
GGCAAGTCCT TCTTTGTGGA CCACAACAGC CGTGCTACCA CTTTTATCGA TCCTAGGATC 
CCACTTCAAA ATGGCCGTCT CCCTAATCAT CTGACTCACA GGCAGCATCT CCAGAGACTT 
CGCAGCTACA GTGCTGGAGA GGCATCAGAA GTCTCCCGGA ATAGAGGCAT ATCTCTGTTG 
ACCAGACCAG GCAACAGCCT AGTAACAGCT ATTCGAAACC AGCACCATCA TGAGTCATTA 
CCTCTAGCTT ACAATGACAA GATTGTCGCA TTTCTACGCC AACCAAACAT TTTTGAGATG 
CTGCAAGAGC GTCAGCCAAG CCTGGCCAGG AACCATGCAC TCAGGGAGAA GATCCATTAC 
ATTCGGACAG AGGGTACACA TGGGCTTGAA AAGCTGTCCT GTGATGCAGA TTTAGTAATT 
CTATTGAGCC TCTTTGAGGA GGATATTATG TCCTACATAC CCCTGCAGGC TGCCTTCCAC 
CCTGGCTATA GCTTCTCTCC TCGCTGTTCA CCCTGCTCCT CACCTCAGAA TTCTCCAGGA 
TTACAGCGAG CTAGTGCACG AGCGCCCTCT CCATATAGGA GAGACTTTGA AGCCAAGCTA 
CGCAATTTCT ATCGAAAACT TGAAGCCAAA GGATATGGAC AAGGTCCAGG TAAAATTAAG 
TTAATTATAC GGCGTGACCA CTTGTTGGAA GGCACCTTCA ATCAGGTGAT GGCTTATTCA 
CGCAAGGAGC TTCAGCGGAA CAAACTCTAT GTCACATTCG TTGGAGAGGA AGGCTTGGAC 
TACAGCGGTC CCTCCCGAGA GTTCTTCTTC CTTCTGTCTC AGGAGCTCTT CAATCCTTAC 
TATGGCTTGT TTGAGTATTC AGCCAACGAC ACTTACACTG TACAGATCAG CCCCATGTCT 
GCGTTTGTGG AAAACCACCT TGAATGGTTC AGGTTCAGCG GTCGTATCCT TGGCCTTGCT 
CTCATTCATC AGTACCTTCT TGATGCTTTC TTCACAAGGC CGTTCTATAA AGCACTACTG 
AGGCTGCCGT GTGATTTAAG TGACCTGGAG TACCTGGATG AGGAGTTTCA CCAGAGTTTA 
CAGTGGATGA AGGATAACAA CATCACTGAT ATCCTGGATC TCACATTCAC CGTGAACGAG 
GAGGTGTTTG GACAGGTGAC AGAAAGGGAG CTGAAATCAG GGGGTTCAAA TACACAGGTG 
ACAGAAAAGA ACAAGAAGGA GTACATTGAG AGAATGGTGA AGTGGCGTGT GGAACGAGGA 
GTGGTTCAGC AAACAGAGGC TCTAGTCCGT GGCTTCTATG AAGTTGTAGA CTCCAGGCTT 
GTCTCCATTT TTGATGCCAG AGAGCTAGAG TTGGTGATTG CAGGTACAGC AGAAATAGAC 
CTCAATGACT GGCGTAACAA CACAGAATAT CGTGGAGGTT ACCATGATGG GCATATTGTC 
ATACGGTGGT TCTGGGCAGC AGTGGAGAGA TTTAACAATG AACAGAGACT ACGGTTACTG 
CAGTTTGTCA CTGGAACATC TAGTGTACCA TATGAGGGTT TTGCAGCCCT TCGAGGGAGC 
AATGGGTTAC GGCGTTTCTG TATAGAAAAA TGGGGGAAAA TAACATCTCT TCCTAGGGCG 
CACACATGTT TCAACCGCTT GGATCTCCCG CCCTACCCAT CTTATTCTAT GCTGTATGAA 
AAGCTGTTGA CAGCTGTTGA AGAAACTAGC ACCTTTGGAC TTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025040959.1
CDS	44..4891
Translation	MWDLKPALAPARGSMICARTGILQNLYQNRFLGLAAMASPSRNSQNRRRCKEPLRYSYNPDQFNNMDLRNNAHETVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIYWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDANSYFVESETKICFKYYHGVSGALRATTPSVTVKNSSAPIFKSIVNEDPVQGQGSRRLISFSLSDFQALGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQEKRSKITCNTVNPIWQGEHFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPDDEEVSISADPESAELQESPVNCTAEEILGEPPCTGTVTSQSTSPEQLNGCHVKSVNVDIPGEVKQNSLNEELAGNREVGVVPADATASLESIPGEVLPSLSALELSEQLALLVCTPPPEIDFRENNKVNSVTQGHEDIFTRMEALDTDEVSADIQTVKYLEKIQDEEGASAQEEEDNKLQLRMSGKRKNRPCSLPVSELETVIASACGEPETPRTHYIRIHNLLHSLPSAQMSSDGEEEPGGGENDEESTVKEVSEKDVVSESETLAADPPPESGTDEYFSRGTVPRSTSVEHLSDLNGQLLDGEHPTAGSESESSSRPYGDNECDTSCYSPSCYSTSCYSTSCYSTSCYSPSCYDSNNRFSSHTRFSSVDSAKISESTVFSSQDDEEEENSAFESVPDSVQSPELDREQVDVSAQWPDELIAHGGNSQRATEILESPVAGPSNRREGDCPLLHNSQPVSQLPSLRPDHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGIRRSGSIQQMEQLNRRYQNIQRTIATERPEDDAVVNNRVERLSTGGGSDSETDSSQPNSEIRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTNSTCLKHMILKIRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGISLLTRPGNSLVTAIRNQHHHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHALREKIHYIRTEGTHGLEKLSCDADLVILLSLFEEDIMSYIPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGYGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGSNTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSIFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_025185174.1
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Definition	Pelodiscus sinensis HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025185174.1

ORF Insert Sequence:

ATGTGGGATC TAAAACCTGC CCTGGCTCCT GCTCGGGGGA GCATGATCTG TGCCCGCACC 
GGCATCCTCC AGAATCTGTA CCAAAACAGG TTCCTAGGCC TGGCTGCCAT GGCGTCTCCA 
TCTAGGAATT CACAGAACCG GCGCCGGTGT AAAGAACCTC TCAGATACAG CTATAACCCT 
GACCAGTTTA ATAACATGGA CCTCAGGAAC AATGCTCATG AGACAGTAAC CATTCCACGA 
TCCACCAGTG ATACTGACCT GGTCACTTCA GACAGCCGGT CTACTCTCAT GGTCAGCAGC 
TCATATTACT CCATTGGACA TTCACAAGAC CTAGTTATCT ATTGGGATAT AAAGGAAGAA 
GTGGATGCAG GGGACTGGAT CGGCATGTAT CTCATTGATG AAGTCTTGTC TGAAAACTTT 
CTGGACTACA AAAACCGTGG GGTCAATGGC TCTCATCGTG GTCAGATTAT CTGGAAGATT 
GATGCCAACT CTTACTTCGT AGAGTCTGAA ACAAAGATCT GCTTCAAATA CTACCATGGA 
GTGAGTGGAG CACTACGAGC TACGACCCCC AGTGTCACAG TGAAAAACAG TTCTGCTCCC 
ATTTTTAAAA GCATCGTCAA TGAAGATCCA GTCCAGGGTC AAGGAAGTCG GAGACTGATC 
AGTTTTTCAC TGTCAGATTT CCAGGCTTTG GGTCTGAAAA AAGGAATGTT CTTTAACCCA 
GACCCATACC TGAAGATTTC CATTCAGCCT GGAAAGCACA GCATCTTCCC AGCACTTCCT 
CACCATGGAC AAGAGAAGAG ATCCAAGATC ACATGTAATA CTGTGAACCC TATCTGGCAA 
GGAGAGCACT TCAGCTTTGT TTCCCTTCCC ACGGATGTTC TGGAAATAGA AGTTAAGGAC 
AAGTTTGCAA AGAGCCGGCC AATCATTAAA CGCTTCTTGG GGAAACTTTC CATGCCAGTG 
CAACGCCTGC TGGAGAGACA CGCAATAGGG GACAGGGTTG TCAGCTACAC TCTGGGACGT 
AGGCTTCCAA CAGATCATGT CAGCGGCCAG CTGCAGTTTC GGTTTGAGAT AACTTCTTCT 
ATCCATCCAG ATGATGAAGA GGTCTCCATT AGTGCAGATC CTGAGTCAGC TGAACTGCAG 
GAAAGCCCTG TGAACTGCAC CGCAGAGGAA ATCCTGGGTG AGCCTCCCTG CACCGGCACA 
GTGACCTCAC AAAGTACATC TCCAGAGCAG CTAAATGGAT GTCATGTGAA AAGTGTCAAT 
GTTGATATCC CTGGGGAAGT CAAACAGAAC AGCTTAAATG AAGAGCTAGC AGGAAATAGG 
GAGGTTGGTG TAGTGCCAGC AGATGCTACT GCGTCCTTGG AATCCATCCC TGGGGAAGTG 
CTGCCTTCCC TGAGTGCTCT GGAACTCTCT GAGCAGCTGG CTCTGCTGGT TTGCACACCT 
CCTCCAGAGA TTGACTTTAG GGAAAATAAT AAAGTCAATT CTGTTACTCA GGGACACGAG 
GACATCTTCA CCCGCATGGA AGCACTAGAT ACCGATGAGG TGAGTGCAGA CATACAGACT 
GTCAAGTACT TGGAGAAAAT TCAGGATGAG GAAGGCGCTT CAGCCCAGGA AGAGGAAGAC 
AACAAGCTGC AGCTAAGAAT GTCAGGGAAA AGGAAAAACA GGCCCTGCTC CTTGCCTGTG 
TCTGAGCTGG AGACGGTGAT TGCATCAGCC TGTGGTGAGC CTGAGACCCC TCGAACGCAC 
TACATTCGGA TCCACAATCT GCTTCACAGC TTGCCCTCAG CACAGATGAG TAGTGATGGA 
GAGGAGGAGC CGGGAGGCGG CGAGAATGAC GAAGAGTCAA CGGTCAAGGA AGTGTCAGAG 
AAGGATGTGG TTAGCGAGTC TGAGACGCTA GCGGCAGATC CTCCCCCTGA AAGCGGAACT 
GATGAGTATT TCAGCCGGGG GACTGTGCCC CGCAGCACCT CAGTTGAACA CCTCTCAGAC 
TTAAATGGGC AGCTGTTGGA TGGAGAACAT CCAACGGCTG GAAGTGAGAG TGAATCAAGT 
TCCAGGCCAT ACGGGGACAA TGAGTGTGAC ACCTCTTGCT ACAGCCCATC CTGCTACAGC 
ACCTCCTGCT ACAGTACGTC CTGCTACAGC ACCTCCTGCT ATAGCCCCTC CTGTTATGAC 
AGTAACAATC GCTTTTCCAG CCATACCCGT TTCTCCTCTG TTGACAGTGC AAAGATTTCT 
GAGAGCACAG TCTTTTCCTC ACAGGATGAT GAGGAAGAGG AAAACAGTGC CTTTGAGTCA 
GTGCCAGATT CAGTGCAAAG CCCTGAACTG GACAGGGAAC AAGTGGATGT CTCCGCCCAG 
TGGCCAGATG AACTCATAGC TCATGGTGGG AATTCACAAA GAGCAACAGA GATCCTAGAA 
TCCCCAGTAG CAGGTCCAAG CAACAGAAGA GAAGGTGATT GTCCTTTACT CCATAATTCT 
CAGCCAGTCA GCCAGCTTCC TTCCCTGAGG CCTGACCATC ATCACTATCC AACTATTGAT 
GAGCCTCTTC CACCAAACTG GGAGGCTCGG ATAGACAGCC ATGGAAGGGT ATTTTATGTG 
GATCACGTGA ATCGAACCAC AACATGGCAG CGTCCTACAG CAGCAGCCAC TCCAGATGGA 
ATACGCAGAT CTGGTTCAAT CCAACAAATG GAGCAGCTCA ATCGGCGGTA TCAAAATATT 
CAGCGGACTA TTGCAACAGA GAGGCCAGAG GATGATGCTG TTGTGAACAA CAGAGTTGAG 
AGGCTCTCCA CTGGAGGAGG AAGTGACTCT GAGACAGACT CTTCCCAGCC AAACTCAGAA 
ATTAGGCGAG AAGGGTCCCT TTCTCCAGTG AATTCTCAAA AAATCACCTT GCTGCTACAG 
TCTCCAGCTG TAAAGTTTAT CACAAACCCT GAATTCTTCA CCGTGCTGCA TGCAAACTAT 
AGTGCCTATC GAGTCTTCAC CAACAGCACC TGCTTGAAGC ACATGATTCT AAAGATCCGC 
AGAGATGCTC GGAATTTTGA GCGCTATCAG CACAATAGGG ACCTGGTAAA TTTTATCAAT 
ATGTTTGCTG ACACCCGACT GGAACTTCCA CGGGGCTGGG AGATCAAAAC AGACCAACAG 
GGCAAGTCCT TCTTTGTGGA CCACAACAGC CGTGCTACCA CTTTTATCGA TCCTAGGATC 
CCACTTCAAA ATGGCCGTCT CCCTAATCAT CTGACTCACA GGCAGCATCT CCAGAGACTT 
CGCAGCTACA GTGCTGGAGA GGCATCAGAA GTCTCCCGGA ATAGAGGCAT ATCTCTGTTG 
ACCAGACCAG GCAACAGCCT AGTAACAGCT ATTCGAAACC AGCACCATCA TGAGTCATTA 
CCTCTAGCTT ACAATGACAA GATTGTCGCA TTTCTACGCC AACCAAACAT TTTTGAGATG 
CTGCAAGAGC GTCAGCCAAG CCTGGCCAGG AACCATGCAC TCAGGGAGAA GATCCATTAC 
ATTCGGACAG AGGGTACACA TGGGCTTGAA AAGCTGTCCT GTGATGCAGA TTTAGTAATT 
CTATTGAGCC TCTTTGAGGA GGATATTATG TCCTACATAC CCCTGCAGGC TGCCTTCCAC 
CCTGGCTATA GCTTCTCTCC TCGCTGTTCA CCCTGCTCCT CACCTCAGAA TTCTCCAGGA 
TTACAGCGAG CTAGTGCACG AGCGCCCTCT CCATATAGGA GAGACTTTGA AGCCAAGCTA 
CGCAATTTCT ATCGAAAACT TGAAGCCAAA GGATATGGAC AAGGTCCAGG TAAAATTAAG 
TTAATTATAC GGCGTGACCA CTTGTTGGAA GGCACCTTCA ATCAGGTGAT GGCTTATTCA 
CGCAAGGAGC TTCAGCGGAA CAAACTCTAT GTCACATTCG TTGGAGAGGA AGGCTTGGAC 
TACAGCGGTC CCTCCCGAGA GTTCTTCTTC CTTCTGTCTC AGGAGCTCTT CAATCCTTAC 
TATGGCTTGT TTGAGTATTC AGCCAACGAC ACTTACACTG TACAGATCAG CCCCATGTCT 
GCGTTTGTGG AAAACCACCT TGAATGGTTC AGGTTCAGCG GTCGTATCCT TGGCCTTGCT 
CTCATTCATC AGTACCTTCT TGATGCTTTC TTCACAAGGC CGTTCTATAA AGCACTACTG 
AGGCTGCCGT GTGATTTAAG TGACCTGGAG TACCTGGATG AGGAGTTTCA CCAGAGTTTA 
CAGTGGATGA AGGATAACAA CATCACTGAT ATCCTGGATC TCACATTCAC CGTGAACGAG 
GAGGTGTTTG GACAGGTGAC AGAAAGGGAG CTGAAATCAG GGGGTTCAAA TACACAGGTG 
ACAGAAAAGA ACAAGAAGGA GTACATTGAG AGAATGGTGA AGTGGCGTGT GGAACGAGGA 
GTGGTTCAGC AAACAGAGGC TCTAGTCCGT GGCTTCTATG AAGTTGTAGA CTCCAGGCTT 
GTCTCCATTT TTGATGCCAG AGAGCTAGAG TTGGTGATTG CAGGTACAGC AGAAATAGAC 
CTCAATGACT GGCGTAACAA CACAGAATAT CGTGGAGGTT ACCATGATGG GCATATTGTC 
ATACGGTGGT TCTGGGCAGC AGTGGAGAGA TTTAACAATG AACAGAGACT ACGGTTACTG 
CAGTTTGTCA CTGGAACATC TAGTGTACCA TATGAGGGTT TTGCAGCCCT TCGAGGGAGC 
AATGGGTTAC GGCGTTTCTG TATAGAAAAA TGGGGGAAAA TAACATCTCT TCCTAGGGCG 
CACACATGTT TCAACCGCTT GGATCTCCCG CCCTACCCAT CTTATTCTAT GCTGTATGAA 
AAGCTGTTGA CAGCTGTTGA AGAAACTAGC ACCTTTGGAC TTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_014574083.1 ) cDNA ORF clone, Pelodiscus sinensis(Chinese soft-shelled turtle) -> XP_014429569.1 Pelodiscus sinensis HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X1, mRNA.

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CloneID	OPe30299
Clone ID Related Accession (Same CDS sequence)	XM_014574083.1 , XM_025185175.1 , XM_014574083.2
Accession Version	XM_014574083.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4803bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-11-02
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Product	E3 ubiquitin-protein ligase HECW1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005855477.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pelodiscus sinensis Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTCC ACCTGTGTAG TGTTAAGAAT CTGTACCAAA ACAGGTTCCT AGGCCTGGCT 
GCCATGGCGT CTCCATCTAG GAATTCACAG AACCGGCGCC GGTGTAAAGA ACCTCTCAGA 
TACAGCTATA ACCCTGACCA GTTTAATAAC ATGGACCTCA GGAACAATGC TCATGAGACA 
GTAACCATTC CACGATCCAC CAGTGATACT GACCTGGTCA CTTCAGACAG CCGGTCTACT 
CTCATGGTCA GCAGCTCATA TTACTCCATT GGACATTCAC AAGACCTAGT TATCTATTGG 
GATATAAAGG AAGAAGTGGA TGCAGGGGAC TGGATCGGCA TGTATCTCAT TGATGAAGTC 
TTGTCTGAAA ACTTTCTGGA CTACAAAAAC CGTGGGGTCA ATGGCTCTCA TCGTGGTCAG 
ATTATCTGGA AGATTGATGC CAACTCTTAC TTCGTAGAGT CTGAAACAAA GATCTGCTTC 
AAATACTACC ATGGAGTGAG TGGAGCACTA CGAGCTACGA CCCCCAGTGT CACAGTGAAA 
AACAGTTCTG CTCCCATTTT TAAAAGCATC GTCAATGAAG ATCCAGTCCA GGGTCAAGGA 
AGTCGGAGAC TGATCAGTTT TTCACTGTCA GATTTCCAGG CTTTGGGTCT GAAAAAAGGA 
ATGTTCTTTA ACCCAGACCC ATACCTGAAG ATTTCCATTC AGCCTGGAAA GCACAGCATC 
TTCCCAGCAC TTCCTCACCA TGGACAAGAG AAGAGATCCA AGATCACATG TAATACTGTG 
AACCCTATCT GGCAAGGAGA GCACTTCAGC TTTGTTTCCC TTCCCACGGA TGTTCTGGAA 
ATAGAAGTTA AGGACAAGTT TGCAAAGAGC CGGCCAATCA TTAAACGCTT CTTGGGGAAA 
CTTTCCATGC CAGTGCAACG CCTGCTGGAG AGACACGCAA TAGGGGACAG GGTTGTCAGC 
TACACTCTGG GACGTAGGCT TCCAACAGAT CATGTCAGCG GCCAGCTGCA GTTTCGGTTT 
GAGATAACTT CTTCTATCCA TCCAGATGAT GAAGAGGTCT CCATTAGTGC AGATCCTGAG 
TCAGCTGAAC TGCAGGAAAG CCCTGTGAAC TGCACCGCAG AGGAAATCCT GGGTGAGCCT 
CCCTGCACCG GCACAGTGAC CTCACAAAGT ACATCTCCAG AGCAGCTAAA TGGATGTCAT 
GTGAAAAGTG TCAATGTTGA TATCCCTGGG GAAGTCAAAC AGAACAGCTT AAATGAAGAG 
CTAGCAGGAA ATAGGGAGGT TGGTGTAGTG CCAGCAGATG CTACTGCGTC CTTGGAATCC 
ATCCCTGGGG AAGTGCTGCC TTCCCTGAGT GCTCTGGAAC TCTCTGAGCA GCTGGCTCTG 
CTGGTTTGCA CACCTCCTCC AGAGATTGAC TTTAGGGAAA ATAATAAAGT CAATTCTGTT 
ACTCAGGGAC ACGAGGACAT CTTCACCCGC ATGGAAGCAC TAGATACCGA TGAGGTGAGT 
GCAGACATAC AGACTGTCAA GTACTTGGAG AAAATTCAGG ATGAGGAAGG CGCTTCAGCC 
CAGGAAGAGG AAGACAACAA GCTGCAGCTA AGAATGTCAG GGAAAAGGAA AAACAGGCCC 
TGCTCCTTGC CTGTGTCTGA GCTGGAGACG GTGATTGCAT CAGCCTGTGG TGAGCCTGAG 
ACCCCTCGAA CGCACTACAT TCGGATCCAC AATCTGCTTC ACAGCTTGCC CTCAGCACAG 
ATGAGTAGTG ATGGAGAGGA GGAGCCGGGA GGCGGCGAGA ATGACGAAGA GTCAACGGTC 
AAGGAAGTGT CAGAGAAGGA TGTGGTTAGC GAGTCTGAGA CGCTAGCGGC AGATCCTCCC 
CCTGAAAGCG GAACTGATGA GTATTTCAGC CGGGGGACTG TGCCCCGCAG CACCTCAGTT 
GAACACCTCT CAGACTTAAA TGGGCAGCTG TTGGATGGAG AACATCCAAC GGCTGGAAGT 
GAGAGTGAAT CAAGTTCCAG GCCATACGGG GACAATGAGT GTGACACCTC TTGCTACAGC 
CCATCCTGCT ACAGCACCTC CTGCTACAGT ACGTCCTGCT ACAGCACCTC CTGCTATAGC 
CCCTCCTGTT ATGACAGTAA CAATCGCTTT TCCAGCCATA CCCGTTTCTC CTCTGTTGAC 
AGTGCAAAGA TTTCTGAGAG CACAGTCTTT TCCTCACAGG ATGATGAGGA AGAGGAAAAC 
AGTGCCTTTG AGTCAGTGCC AGATTCAGTG CAAAGCCCTG AACTGGACAG GGAACAAGTG 
GATGTCTCCG CCCAGTGGCC AGATGAACTC ATAGCTCATG GTGGGAATTC ACAAAGAGCA 
ACAGAGATCC TAGAATCCCC AGTAGCAGGT CCAAGCAACA GAAGAGAAGG TGATTGTCCT 
TTACTCCATA ATTCTCAGCC AGTCAGCCAG CTTCCTTCCC TGAGGCCTGA CCATCATCAC 
TATCCAACTA TTGATGAGCC TCTTCCACCA AACTGGGAGG CTCGGATAGA CAGCCATGGA 
AGGGTATTTT ATGTGGATCA CGTGAATCGA ACCACAACAT GGCAGCGTCC TACAGCAGCA 
GCCACTCCAG ATGGAATACG CAGATCTGGT TCAATCCAAC AAATGGAGCA GCTCAATCGG 
CGGTATCAAA ATATTCAGCG GACTATTGCA ACAGAGAGGC CAGAGGATGA TGCTGTTGTG 
AACAACAGAG TTGAGAGGCT CTCCACTGGA GGAGGAAGTG ACTCTGAGAC AGACTCTTCC 
CAGCCAAACT CAGAAATTAG GCGAGAAGGG TCCCTTTCTC CAGTGAATTC TCAAAAAATC 
ACCTTGCTGC TACAGTCTCC AGCTGTAAAG TTTATCACAA ACCCTGAATT CTTCACCGTG 
CTGCATGCAA ACTATAGTGC CTATCGAGTC TTCACCAACA GCACCTGCTT GAAGCACATG 
ATTCTAAAGA TCCGCAGAGA TGCTCGGAAT TTTGAGCGCT ATCAGCACAA TAGGGACCTG 
GTAAATTTTA TCAATATGTT TGCTGACACC CGACTGGAAC TTCCACGGGG CTGGGAGATC 
AAAACAGACC AACAGGGCAA GTCCTTCTTT GTGGACCACA ACAGCCGTGC TACCACTTTT 
ATCGATCCTA GGATCCCACT TCAAAATGGC CGTCTCCCTA ATCATCTGAC TCACAGGCAG 
CATCTCCAGA GACTTCGCAG CTACAGTGCT GGAGAGGCAT CAGAAGTCTC CCGGAATAGA 
GGCATATCTC TGTTGACCAG ACCAGGCAAC AGCCTAGTAA CAGCTATTCG AAACCAGCAC 
CATCATGAGT CATTACCTCT AGCTTACAAT GACAAGATTG TCGCATTTCT ACGCCAACCA 
AACATTTTTG AGATGCTGCA AGAGCGTCAG CCAAGCCTGG CCAGGAACCA TGCACTCAGG 
GAGAAGATCC ATTACATTCG GACAGAGGGT ACACATGGGC TTGAAAAGCT GTCCTGTGAT 
GCAGATTTAG TAATTCTATT GAGCCTCTTT GAGGAGGATA TTATGTCCTA CATACCCCTG 
CAGGCTGCCT TCCACCCTGG CTATAGCTTC TCTCCTCGCT GTTCACCCTG CTCCTCACCT 
CAGAATTCTC CAGGATTACA GCGAGCTAGT GCACGAGCGC CCTCTCCATA TAGGAGAGAC 
TTTGAAGCCA AGCTACGCAA TTTCTATCGA AAACTTGAAG CCAAAGGATA TGGACAAGGT 
CCAGGTAAAA TTAAGTTAAT TATACGGCGT GACCACTTGT TGGAAGGCAC CTTCAATCAG 
GTGATGGCTT ATTCACGCAA GGAGCTTCAG CGGAACAAAC TCTATGTCAC ATTCGTTGGA 
GAGGAAGGCT TGGACTACAG CGGTCCCTCC CGAGAGTTCT TCTTCCTTCT GTCTCAGGAG 
CTCTTCAATC CTTACTATGG CTTGTTTGAG TATTCAGCCA ACGACACTTA CACTGTACAG 
ATCAGCCCCA TGTCTGCGTT TGTGGAAAAC CACCTTGAAT GGTTCAGGTT CAGCGGTCGT 
ATCCTTGGCC TTGCTCTCAT TCATCAGTAC CTTCTTGATG CTTTCTTCAC AAGGCCGTTC 
TATAAAGCAC TACTGAGGCT GCCGTGTGAT TTAAGTGACC TGGAGTACCT GGATGAGGAG 
TTTCACCAGA GTTTACAGTG GATGAAGGAT AACAACATCA CTGATATCCT GGATCTCACA 
TTCACCGTGA ACGAGGAGGT GTTTGGACAG GTGACAGAAA GGGAGCTGAA ATCAGGGGGT 
TCAAATACAC AGGTGACAGA AAAGAACAAG AAGGAGTACA TTGAGAGAAT GGTGAAGTGG 
CGTGTGGAAC GAGGAGTGGT TCAGCAAACA GAGGCTCTAG TCCGTGGCTT CTATGAAGTT 
GTAGACTCCA GGCTTGTCTC CATTTTTGAT GCCAGAGAGC TAGAGTTGGT GATTGCAGGT 
ACAGCAGAAA TAGACCTCAA TGACTGGCGT AACAACACAG AATATCGTGG AGGTTACCAT 
GATGGGCATA TTGTCATACG GTGGTTCTGG GCAGCAGTGG AGAGATTTAA CAATGAACAG 
AGACTACGGT TACTGCAGTT TGTCACTGGA ACATCTAGTG TACCATATGA GGGTTTTGCA 
GCCCTTCGAG GGAGCAATGG GTTACGGCGT TTCTGTATAG AAAAATGGGG GAAAATAACA 
TCTCTTCCTA GGGCGCACAC ATGTTTCAAC CGCTTGGATC TCCCGCCCTA CCCATCTTAT 
TCTATGCTGT ATGAAAAGCT GTTGACAGCT GTTGAAGAAA CTAGCACCTT TGGACTTGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014429569.1
CDS	111..4913
Translation	MLLHLCSVKNLYQNRFLGLAAMASPSRNSQNRRRCKEPLRYSYNPDQFNNMDLRNNAHETVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIYWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDANSYFVESETKICFKYYHGVSGALRATTPSVTVKNSSAPIFKSIVNEDPVQGQGSRRLISFSLSDFQALGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQEKRSKITCNTVNPIWQGEHFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPDDEEVSISADPESAELQESPVNCTAEEILGEPPCTGTVTSQSTSPEQLNGCHVKSVNVDIPGEVKQNSLNEELAGNREVGVVPADATASLESIPGEVLPSLSALELSEQLALLVCTPPPEIDFRENNKVNSVTQGHEDIFTRMEALDTDEVSADIQTVKYLEKIQDEEGASAQEEEDNKLQLRMSGKRKNRPCSLPVSELETVIASACGEPETPRTHYIRIHNLLHSLPSAQMSSDGEEEPGGGENDEESTVKEVSEKDVVSESETLAADPPPESGTDEYFSRGTVPRSTSVEHLSDLNGQLLDGEHPTAGSESESSSRPYGDNECDTSCYSPSCYSTSCYSTSCYSTSCYSPSCYDSNNRFSSHTRFSSVDSAKISESTVFSSQDDEEEENSAFESVPDSVQSPELDREQVDVSAQWPDELIAHGGNSQRATEILESPVAGPSNRREGDCPLLHNSQPVSQLPSLRPDHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGIRRSGSIQQMEQLNRRYQNIQRTIATERPEDDAVVNNRVERLSTGGGSDSETDSSQPNSEIRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTNSTCLKHMILKIRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGISLLTRPGNSLVTAIRNQHHHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHALREKIHYIRTEGTHGLEKLSCDADLVILLSLFEEDIMSYIPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGYGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGSNTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSIFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_014574083.1
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Definition	Pelodiscus sinensis HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014574083.1

ORF Insert Sequence:

ATGCTGCTCC ACCTGTGTAG TGTTAAGAAT CTGTACCAAA ACAGGTTCCT AGGCCTGGCT 
GCCATGGCGT CTCCATCTAG GAATTCACAG AACCGGCGCC GGTGTAAAGA ACCTCTCAGA 
TACAGCTATA ACCCTGACCA GTTTAATAAC ATGGACCTCA GGAACAATGC TCATGAGACA 
GTAACCATTC CACGATCCAC CAGTGATACT GACCTGGTCA CTTCAGACAG CCGGTCTACT 
CTCATGGTCA GCAGCTCATA TTACTCCATT GGACATTCAC AAGACCTAGT TATCTATTGG 
GATATAAAGG AAGAAGTGGA TGCAGGGGAC TGGATCGGCA TGTATCTCAT TGATGAAGTC 
TTGTCTGAAA ACTTTCTGGA CTACAAAAAC CGTGGGGTCA ATGGCTCTCA TCGTGGTCAG 
ATTATCTGGA AGATTGATGC CAACTCTTAC TTCGTAGAGT CTGAAACAAA GATCTGCTTC 
AAATACTACC ATGGAGTGAG TGGAGCACTA CGAGCTACGA CCCCCAGTGT CACAGTGAAA 
AACAGTTCTG CTCCCATTTT TAAAAGCATC GTCAATGAAG ATCCAGTCCA GGGTCAAGGA 
AGTCGGAGAC TGATCAGTTT TTCACTGTCA GATTTCCAGG CTTTGGGTCT GAAAAAAGGA 
ATGTTCTTTA ACCCAGACCC ATACCTGAAG ATTTCCATTC AGCCTGGAAA GCACAGCATC 
TTCCCAGCAC TTCCTCACCA TGGACAAGAG AAGAGATCCA AGATCACATG TAATACTGTG 
AACCCTATCT GGCAAGGAGA GCACTTCAGC TTTGTTTCCC TTCCCACGGA TGTTCTGGAA 
ATAGAAGTTA AGGACAAGTT TGCAAAGAGC CGGCCAATCA TTAAACGCTT CTTGGGGAAA 
CTTTCCATGC CAGTGCAACG CCTGCTGGAG AGACACGCAA TAGGGGACAG GGTTGTCAGC 
TACACTCTGG GACGTAGGCT TCCAACAGAT CATGTCAGCG GCCAGCTGCA GTTTCGGTTT 
GAGATAACTT CTTCTATCCA TCCAGATGAT GAAGAGGTCT CCATTAGTGC AGATCCTGAG 
TCAGCTGAAC TGCAGGAAAG CCCTGTGAAC TGCACCGCAG AGGAAATCCT GGGTGAGCCT 
CCCTGCACCG GCACAGTGAC CTCACAAAGT ACATCTCCAG AGCAGCTAAA TGGATGTCAT 
GTGAAAAGTG TCAATGTTGA TATCCCTGGG GAAGTCAAAC AGAACAGCTT AAATGAAGAG 
CTAGCAGGAA ATAGGGAGGT TGGTGTAGTG CCAGCAGATG CTACTGCGTC CTTGGAATCC 
ATCCCTGGGG AAGTGCTGCC TTCCCTGAGT GCTCTGGAAC TCTCTGAGCA GCTGGCTCTG 
CTGGTTTGCA CACCTCCTCC AGAGATTGAC TTTAGGGAAA ATAATAAAGT CAATTCTGTT 
ACTCAGGGAC ACGAGGACAT CTTCACCCGC ATGGAAGCAC TAGATACCGA TGAGGTGAGT 
GCAGACATAC AGACTGTCAA GTACTTGGAG AAAATTCAGG ATGAGGAAGG CGCTTCAGCC 
CAGGAAGAGG AAGACAACAA GCTGCAGCTA AGAATGTCAG GGAAAAGGAA AAACAGGCCC 
TGCTCCTTGC CTGTGTCTGA GCTGGAGACG GTGATTGCAT CAGCCTGTGG TGAGCCTGAG 
ACCCCTCGAA CGCACTACAT TCGGATCCAC AATCTGCTTC ACAGCTTGCC CTCAGCACAG 
ATGAGTAGTG ATGGAGAGGA GGAGCCGGGA GGCGGCGAGA ATGACGAAGA GTCAACGGTC 
AAGGAAGTGT CAGAGAAGGA TGTGGTTAGC GAGTCTGAGA CGCTAGCGGC AGATCCTCCC 
CCTGAAAGCG GAACTGATGA GTATTTCAGC CGGGGGACTG TGCCCCGCAG CACCTCAGTT 
GAACACCTCT CAGACTTAAA TGGGCAGCTG TTGGATGGAG AACATCCAAC GGCTGGAAGT 
GAGAGTGAAT CAAGTTCCAG GCCATACGGG GACAATGAGT GTGACACCTC TTGCTACAGC 
CCATCCTGCT ACAGCACCTC CTGCTACAGT ACGTCCTGCT ACAGCACCTC CTGCTATAGC 
CCCTCCTGTT ATGACAGTAA CAATCGCTTT TCCAGCCATA CCCGTTTCTC CTCTGTTGAC 
AGTGCAAAGA TTTCTGAGAG CACAGTCTTT TCCTCACAGG ATGATGAGGA AGAGGAAAAC 
AGTGCCTTTG AGTCAGTGCC AGATTCAGTG CAAAGCCCTG AACTGGACAG GGAACAAGTG 
GATGTCTCCG CCCAGTGGCC AGATGAACTC ATAGCTCATG GTGGGAATTC ACAAAGAGCA 
ACAGAGATCC TAGAATCCCC AGTAGCAGGT CCAAGCAACA GAAGAGAAGG TGATTGTCCT 
TTACTCCATA ATTCTCAGCC AGTCAGCCAG CTTCCTTCCC TGAGGCCTGA CCATCATCAC 
TATCCAACTA TTGATGAGCC TCTTCCACCA AACTGGGAGG CTCGGATAGA CAGCCATGGA 
AGGGTATTTT ATGTGGATCA CGTGAATCGA ACCACAACAT GGCAGCGTCC TACAGCAGCA 
GCCACTCCAG ATGGAATACG CAGATCTGGT TCAATCCAAC AAATGGAGCA GCTCAATCGG 
CGGTATCAAA ATATTCAGCG GACTATTGCA ACAGAGAGGC CAGAGGATGA TGCTGTTGTG 
AACAACAGAG TTGAGAGGCT CTCCACTGGA GGAGGAAGTG ACTCTGAGAC AGACTCTTCC 
CAGCCAAACT CAGAAATTAG GCGAGAAGGG TCCCTTTCTC CAGTGAATTC TCAAAAAATC 
ACCTTGCTGC TACAGTCTCC AGCTGTAAAG TTTATCACAA ACCCTGAATT CTTCACCGTG 
CTGCATGCAA ACTATAGTGC CTATCGAGTC TTCACCAACA GCACCTGCTT GAAGCACATG 
ATTCTAAAGA TCCGCAGAGA TGCTCGGAAT TTTGAGCGCT ATCAGCACAA TAGGGACCTG 
GTAAATTTTA TCAATATGTT TGCTGACACC CGACTGGAAC TTCCACGGGG CTGGGAGATC 
AAAACAGACC AACAGGGCAA GTCCTTCTTT GTGGACCACA ACAGCCGTGC TACCACTTTT 
ATCGATCCTA GGATCCCACT TCAAAATGGC CGTCTCCCTA ATCATCTGAC TCACAGGCAG 
CATCTCCAGA GACTTCGCAG CTACAGTGCT GGAGAGGCAT CAGAAGTCTC CCGGAATAGA 
GGCATATCTC TGTTGACCAG ACCAGGCAAC AGCCTAGTAA CAGCTATTCG AAACCAGCAC 
CATCATGAGT CATTACCTCT AGCTTACAAT GACAAGATTG TCGCATTTCT ACGCCAACCA 
AACATTTTTG AGATGCTGCA AGAGCGTCAG CCAAGCCTGG CCAGGAACCA TGCACTCAGG 
GAGAAGATCC ATTACATTCG GACAGAGGGT ACACATGGGC TTGAAAAGCT GTCCTGTGAT 
GCAGATTTAG TAATTCTATT GAGCCTCTTT GAGGAGGATA TTATGTCCTA CATACCCCTG 
CAGGCTGCCT TCCACCCTGG CTATAGCTTC TCTCCTCGCT GTTCACCCTG CTCCTCACCT 
CAGAATTCTC CAGGATTACA GCGAGCTAGT GCACGAGCGC CCTCTCCATA TAGGAGAGAC 
TTTGAAGCCA AGCTACGCAA TTTCTATCGA AAACTTGAAG CCAAAGGATA TGGACAAGGT 
CCAGGTAAAA TTAAGTTAAT TATACGGCGT GACCACTTGT TGGAAGGCAC CTTCAATCAG 
GTGATGGCTT ATTCACGCAA GGAGCTTCAG CGGAACAAAC TCTATGTCAC ATTCGTTGGA 
GAGGAAGGCT TGGACTACAG CGGTCCCTCC CGAGAGTTCT TCTTCCTTCT GTCTCAGGAG 
CTCTTCAATC CTTACTATGG CTTGTTTGAG TATTCAGCCA ACGACACTTA CACTGTACAG 
ATCAGCCCCA TGTCTGCGTT TGTGGAAAAC CACCTTGAAT GGTTCAGGTT CAGCGGTCGT 
ATCCTTGGCC TTGCTCTCAT TCATCAGTAC CTTCTTGATG CTTTCTTCAC AAGGCCGTTC 
TATAAAGCAC TACTGAGGCT GCCGTGTGAT TTAAGTGACC TGGAGTACCT GGATGAGGAG 
TTTCACCAGA GTTTACAGTG GATGAAGGAT AACAACATCA CTGATATCCT GGATCTCACA 
TTCACCGTGA ACGAGGAGGT GTTTGGACAG GTGACAGAAA GGGAGCTGAA ATCAGGGGGT 
TCAAATACAC AGGTGACAGA AAAGAACAAG AAGGAGTACA TTGAGAGAAT GGTGAAGTGG 
CGTGTGGAAC GAGGAGTGGT TCAGCAAACA GAGGCTCTAG TCCGTGGCTT CTATGAAGTT 
GTAGACTCCA GGCTTGTCTC CATTTTTGAT GCCAGAGAGC TAGAGTTGGT GATTGCAGGT 
ACAGCAGAAA TAGACCTCAA TGACTGGCGT AACAACACAG AATATCGTGG AGGTTACCAT 
GATGGGCATA TTGTCATACG GTGGTTCTGG GCAGCAGTGG AGAGATTTAA CAATGAACAG 
AGACTACGGT TACTGCAGTT TGTCACTGGA ACATCTAGTG TACCATATGA GGGTTTTGCA 
GCCCTTCGAG GGAGCAATGG GTTACGGCGT TTCTGTATAG AAAAATGGGG GAAAATAACA 
TCTCTTCCTA GGGCGCACAC ATGTTTCAAC CGCTTGGATC TCCCGCCCTA CCCATCTTAT 
TCTATGCTGT ATGAAAAGCT GTTGACAGCT GTTGAAGAAA CTAGCACCTT TGGACTTGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_025185175.1 ) cDNA ORF clone, Pelodiscus sinensis(Chinese soft-shelled turtle) -> XP_025040960.1 Pelodiscus sinensis HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X3, mRNA.

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CloneID	OPe30299
Clone ID Related Accession (Same CDS sequence)	XM_014574083.1 , XM_025185175.1 , XM_014574083.2
Accession Version	XM_025185175.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4803bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-03
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Product	E3 ubiquitin-protein ligase HECW1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005855477.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pelodiscus sinensis Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTCC ACCTGTGTAG TGTTAAGAAT CTGTACCAAA ACAGGTTCCT AGGCCTGGCT 
GCCATGGCGT CTCCATCTAG GAATTCACAG AACCGGCGCC GGTGTAAAGA ACCTCTCAGA 
TACAGCTATA ACCCTGACCA GTTTAATAAC ATGGACCTCA GGAACAATGC TCATGAGACA 
GTAACCATTC CACGATCCAC CAGTGATACT GACCTGGTCA CTTCAGACAG CCGGTCTACT 
CTCATGGTCA GCAGCTCATA TTACTCCATT GGACATTCAC AAGACCTAGT TATCTATTGG 
GATATAAAGG AAGAAGTGGA TGCAGGGGAC TGGATCGGCA TGTATCTCAT TGATGAAGTC 
TTGTCTGAAA ACTTTCTGGA CTACAAAAAC CGTGGGGTCA ATGGCTCTCA TCGTGGTCAG 
ATTATCTGGA AGATTGATGC CAACTCTTAC TTCGTAGAGT CTGAAACAAA GATCTGCTTC 
AAATACTACC ATGGAGTGAG TGGAGCACTA CGAGCTACGA CCCCCAGTGT CACAGTGAAA 
AACAGTTCTG CTCCCATTTT TAAAAGCATC GTCAATGAAG ATCCAGTCCA GGGTCAAGGA 
AGTCGGAGAC TGATCAGTTT TTCACTGTCA GATTTCCAGG CTTTGGGTCT GAAAAAAGGA 
ATGTTCTTTA ACCCAGACCC ATACCTGAAG ATTTCCATTC AGCCTGGAAA GCACAGCATC 
TTCCCAGCAC TTCCTCACCA TGGACAAGAG AAGAGATCCA AGATCACATG TAATACTGTG 
AACCCTATCT GGCAAGGAGA GCACTTCAGC TTTGTTTCCC TTCCCACGGA TGTTCTGGAA 
ATAGAAGTTA AGGACAAGTT TGCAAAGAGC CGGCCAATCA TTAAACGCTT CTTGGGGAAA 
CTTTCCATGC CAGTGCAACG CCTGCTGGAG AGACACGCAA TAGGGGACAG GGTTGTCAGC 
TACACTCTGG GACGTAGGCT TCCAACAGAT CATGTCAGCG GCCAGCTGCA GTTTCGGTTT 
GAGATAACTT CTTCTATCCA TCCAGATGAT GAAGAGGTCT CCATTAGTGC AGATCCTGAG 
TCAGCTGAAC TGCAGGAAAG CCCTGTGAAC TGCACCGCAG AGGAAATCCT GGGTGAGCCT 
CCCTGCACCG GCACAGTGAC CTCACAAAGT ACATCTCCAG AGCAGCTAAA TGGATGTCAT 
GTGAAAAGTG TCAATGTTGA TATCCCTGGG GAAGTCAAAC AGAACAGCTT AAATGAAGAG 
CTAGCAGGAA ATAGGGAGGT TGGTGTAGTG CCAGCAGATG CTACTGCGTC CTTGGAATCC 
ATCCCTGGGG AAGTGCTGCC TTCCCTGAGT GCTCTGGAAC TCTCTGAGCA GCTGGCTCTG 
CTGGTTTGCA CACCTCCTCC AGAGATTGAC TTTAGGGAAA ATAATAAAGT CAATTCTGTT 
ACTCAGGGAC ACGAGGACAT CTTCACCCGC ATGGAAGCAC TAGATACCGA TGAGGTGAGT 
GCAGACATAC AGACTGTCAA GTACTTGGAG AAAATTCAGG ATGAGGAAGG CGCTTCAGCC 
CAGGAAGAGG AAGACAACAA GCTGCAGCTA AGAATGTCAG GGAAAAGGAA AAACAGGCCC 
TGCTCCTTGC CTGTGTCTGA GCTGGAGACG GTGATTGCAT CAGCCTGTGG TGAGCCTGAG 
ACCCCTCGAA CGCACTACAT TCGGATCCAC AATCTGCTTC ACAGCTTGCC CTCAGCACAG 
ATGAGTAGTG ATGGAGAGGA GGAGCCGGGA GGCGGCGAGA ATGACGAAGA GTCAACGGTC 
AAGGAAGTGT CAGAGAAGGA TGTGGTTAGC GAGTCTGAGA CGCTAGCGGC AGATCCTCCC 
CCTGAAAGCG GAACTGATGA GTATTTCAGC CGGGGGACTG TGCCCCGCAG CACCTCAGTT 
GAACACCTCT CAGACTTAAA TGGGCAGCTG TTGGATGGAG AACATCCAAC GGCTGGAAGT 
GAGAGTGAAT CAAGTTCCAG GCCATACGGG GACAATGAGT GTGACACCTC TTGCTACAGC 
CCATCCTGCT ACAGCACCTC CTGCTACAGT ACGTCCTGCT ACAGCACCTC CTGCTATAGC 
CCCTCCTGTT ATGACAGTAA CAATCGCTTT TCCAGCCATA CCCGTTTCTC CTCTGTTGAC 
AGTGCAAAGA TTTCTGAGAG CACAGTCTTT TCCTCACAGG ATGATGAGGA AGAGGAAAAC 
AGTGCCTTTG AGTCAGTGCC AGATTCAGTG CAAAGCCCTG AACTGGACAG GGAACAAGTG 
GATGTCTCCG CCCAGTGGCC AGATGAACTC ATAGCTCATG GTGGGAATTC ACAAAGAGCA 
ACAGAGATCC TAGAATCCCC AGTAGCAGGT CCAAGCAACA GAAGAGAAGG TGATTGTCCT 
TTACTCCATA ATTCTCAGCC AGTCAGCCAG CTTCCTTCCC TGAGGCCTGA CCATCATCAC 
TATCCAACTA TTGATGAGCC TCTTCCACCA AACTGGGAGG CTCGGATAGA CAGCCATGGA 
AGGGTATTTT ATGTGGATCA CGTGAATCGA ACCACAACAT GGCAGCGTCC TACAGCAGCA 
GCCACTCCAG ATGGAATACG CAGATCTGGT TCAATCCAAC AAATGGAGCA GCTCAATCGG 
CGGTATCAAA ATATTCAGCG GACTATTGCA ACAGAGAGGC CAGAGGATGA TGCTGTTGTG 
AACAACAGAG TTGAGAGGCT CTCCACTGGA GGAGGAAGTG ACTCTGAGAC AGACTCTTCC 
CAGCCAAACT CAGAAATTAG GCGAGAAGGG TCCCTTTCTC CAGTGAATTC TCAAAAAATC 
ACCTTGCTGC TACAGTCTCC AGCTGTAAAG TTTATCACAA ACCCTGAATT CTTCACCGTG 
CTGCATGCAA ACTATAGTGC CTATCGAGTC TTCACCAACA GCACCTGCTT GAAGCACATG 
ATTCTAAAGA TCCGCAGAGA TGCTCGGAAT TTTGAGCGCT ATCAGCACAA TAGGGACCTG 
GTAAATTTTA TCAATATGTT TGCTGACACC CGACTGGAAC TTCCACGGGG CTGGGAGATC 
AAAACAGACC AACAGGGCAA GTCCTTCTTT GTGGACCACA ACAGCCGTGC TACCACTTTT 
ATCGATCCTA GGATCCCACT TCAAAATGGC CGTCTCCCTA ATCATCTGAC TCACAGGCAG 
CATCTCCAGA GACTTCGCAG CTACAGTGCT GGAGAGGCAT CAGAAGTCTC CCGGAATAGA 
GGCATATCTC TGTTGACCAG ACCAGGCAAC AGCCTAGTAA CAGCTATTCG AAACCAGCAC 
CATCATGAGT CATTACCTCT AGCTTACAAT GACAAGATTG TCGCATTTCT ACGCCAACCA 
AACATTTTTG AGATGCTGCA AGAGCGTCAG CCAAGCCTGG CCAGGAACCA TGCACTCAGG 
GAGAAGATCC ATTACATTCG GACAGAGGGT ACACATGGGC TTGAAAAGCT GTCCTGTGAT 
GCAGATTTAG TAATTCTATT GAGCCTCTTT GAGGAGGATA TTATGTCCTA CATACCCCTG 
CAGGCTGCCT TCCACCCTGG CTATAGCTTC TCTCCTCGCT GTTCACCCTG CTCCTCACCT 
CAGAATTCTC CAGGATTACA GCGAGCTAGT GCACGAGCGC CCTCTCCATA TAGGAGAGAC 
TTTGAAGCCA AGCTACGCAA TTTCTATCGA AAACTTGAAG CCAAAGGATA TGGACAAGGT 
CCAGGTAAAA TTAAGTTAAT TATACGGCGT GACCACTTGT TGGAAGGCAC CTTCAATCAG 
GTGATGGCTT ATTCACGCAA GGAGCTTCAG CGGAACAAAC TCTATGTCAC ATTCGTTGGA 
GAGGAAGGCT TGGACTACAG CGGTCCCTCC CGAGAGTTCT TCTTCCTTCT GTCTCAGGAG 
CTCTTCAATC CTTACTATGG CTTGTTTGAG TATTCAGCCA ACGACACTTA CACTGTACAG 
ATCAGCCCCA TGTCTGCGTT TGTGGAAAAC CACCTTGAAT GGTTCAGGTT CAGCGGTCGT 
ATCCTTGGCC TTGCTCTCAT TCATCAGTAC CTTCTTGATG CTTTCTTCAC AAGGCCGTTC 
TATAAAGCAC TACTGAGGCT GCCGTGTGAT TTAAGTGACC TGGAGTACCT GGATGAGGAG 
TTTCACCAGA GTTTACAGTG GATGAAGGAT AACAACATCA CTGATATCCT GGATCTCACA 
TTCACCGTGA ACGAGGAGGT GTTTGGACAG GTGACAGAAA GGGAGCTGAA ATCAGGGGGT 
TCAAATACAC AGGTGACAGA AAAGAACAAG AAGGAGTACA TTGAGAGAAT GGTGAAGTGG 
CGTGTGGAAC GAGGAGTGGT TCAGCAAACA GAGGCTCTAG TCCGTGGCTT CTATGAAGTT 
GTAGACTCCA GGCTTGTCTC CATTTTTGAT GCCAGAGAGC TAGAGTTGGT GATTGCAGGT 
ACAGCAGAAA TAGACCTCAA TGACTGGCGT AACAACACAG AATATCGTGG AGGTTACCAT 
GATGGGCATA TTGTCATACG GTGGTTCTGG GCAGCAGTGG AGAGATTTAA CAATGAACAG 
AGACTACGGT TACTGCAGTT TGTCACTGGA ACATCTAGTG TACCATATGA GGGTTTTGCA 
GCCCTTCGAG GGAGCAATGG GTTACGGCGT TTCTGTATAG AAAAATGGGG GAAAATAACA 
TCTCTTCCTA GGGCGCACAC ATGTTTCAAC CGCTTGGATC TCCCGCCCTA CCCATCTTAT 
TCTATGCTGT ATGAAAAGCT GTTGACAGCT GTTGAAGAAA CTAGCACCTT TGGACTTGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025040960.1
CDS	493..5295
Translation	MLLHLCSVKNLYQNRFLGLAAMASPSRNSQNRRRCKEPLRYSYNPDQFNNMDLRNNAHETVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIYWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDANSYFVESETKICFKYYHGVSGALRATTPSVTVKNSSAPIFKSIVNEDPVQGQGSRRLISFSLSDFQALGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQEKRSKITCNTVNPIWQGEHFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPDDEEVSISADPESAELQESPVNCTAEEILGEPPCTGTVTSQSTSPEQLNGCHVKSVNVDIPGEVKQNSLNEELAGNREVGVVPADATASLESIPGEVLPSLSALELSEQLALLVCTPPPEIDFRENNKVNSVTQGHEDIFTRMEALDTDEVSADIQTVKYLEKIQDEEGASAQEEEDNKLQLRMSGKRKNRPCSLPVSELETVIASACGEPETPRTHYIRIHNLLHSLPSAQMSSDGEEEPGGGENDEESTVKEVSEKDVVSESETLAADPPPESGTDEYFSRGTVPRSTSVEHLSDLNGQLLDGEHPTAGSESESSSRPYGDNECDTSCYSPSCYSTSCYSTSCYSTSCYSPSCYDSNNRFSSHTRFSSVDSAKISESTVFSSQDDEEEENSAFESVPDSVQSPELDREQVDVSAQWPDELIAHGGNSQRATEILESPVAGPSNRREGDCPLLHNSQPVSQLPSLRPDHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGIRRSGSIQQMEQLNRRYQNIQRTIATERPEDDAVVNNRVERLSTGGGSDSETDSSQPNSEIRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTNSTCLKHMILKIRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGISLLTRPGNSLVTAIRNQHHHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHALREKIHYIRTEGTHGLEKLSCDADLVILLSLFEEDIMSYIPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGYGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGSNTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSIFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_025185175.1
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Definition	Pelodiscus sinensis HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025185175.1

ORF Insert Sequence:

ATGCTGCTCC ACCTGTGTAG TGTTAAGAAT CTGTACCAAA ACAGGTTCCT AGGCCTGGCT 
GCCATGGCGT CTCCATCTAG GAATTCACAG AACCGGCGCC GGTGTAAAGA ACCTCTCAGA 
TACAGCTATA ACCCTGACCA GTTTAATAAC ATGGACCTCA GGAACAATGC TCATGAGACA 
GTAACCATTC CACGATCCAC CAGTGATACT GACCTGGTCA CTTCAGACAG CCGGTCTACT 
CTCATGGTCA GCAGCTCATA TTACTCCATT GGACATTCAC AAGACCTAGT TATCTATTGG 
GATATAAAGG AAGAAGTGGA TGCAGGGGAC TGGATCGGCA TGTATCTCAT TGATGAAGTC 
TTGTCTGAAA ACTTTCTGGA CTACAAAAAC CGTGGGGTCA ATGGCTCTCA TCGTGGTCAG 
ATTATCTGGA AGATTGATGC CAACTCTTAC TTCGTAGAGT CTGAAACAAA GATCTGCTTC 
AAATACTACC ATGGAGTGAG TGGAGCACTA CGAGCTACGA CCCCCAGTGT CACAGTGAAA 
AACAGTTCTG CTCCCATTTT TAAAAGCATC GTCAATGAAG ATCCAGTCCA GGGTCAAGGA 
AGTCGGAGAC TGATCAGTTT TTCACTGTCA GATTTCCAGG CTTTGGGTCT GAAAAAAGGA 
ATGTTCTTTA ACCCAGACCC ATACCTGAAG ATTTCCATTC AGCCTGGAAA GCACAGCATC 
TTCCCAGCAC TTCCTCACCA TGGACAAGAG AAGAGATCCA AGATCACATG TAATACTGTG 
AACCCTATCT GGCAAGGAGA GCACTTCAGC TTTGTTTCCC TTCCCACGGA TGTTCTGGAA 
ATAGAAGTTA AGGACAAGTT TGCAAAGAGC CGGCCAATCA TTAAACGCTT CTTGGGGAAA 
CTTTCCATGC CAGTGCAACG CCTGCTGGAG AGACACGCAA TAGGGGACAG GGTTGTCAGC 
TACACTCTGG GACGTAGGCT TCCAACAGAT CATGTCAGCG GCCAGCTGCA GTTTCGGTTT 
GAGATAACTT CTTCTATCCA TCCAGATGAT GAAGAGGTCT CCATTAGTGC AGATCCTGAG 
TCAGCTGAAC TGCAGGAAAG CCCTGTGAAC TGCACCGCAG AGGAAATCCT GGGTGAGCCT 
CCCTGCACCG GCACAGTGAC CTCACAAAGT ACATCTCCAG AGCAGCTAAA TGGATGTCAT 
GTGAAAAGTG TCAATGTTGA TATCCCTGGG GAAGTCAAAC AGAACAGCTT AAATGAAGAG 
CTAGCAGGAA ATAGGGAGGT TGGTGTAGTG CCAGCAGATG CTACTGCGTC CTTGGAATCC 
ATCCCTGGGG AAGTGCTGCC TTCCCTGAGT GCTCTGGAAC TCTCTGAGCA GCTGGCTCTG 
CTGGTTTGCA CACCTCCTCC AGAGATTGAC TTTAGGGAAA ATAATAAAGT CAATTCTGTT 
ACTCAGGGAC ACGAGGACAT CTTCACCCGC ATGGAAGCAC TAGATACCGA TGAGGTGAGT 
GCAGACATAC AGACTGTCAA GTACTTGGAG AAAATTCAGG ATGAGGAAGG CGCTTCAGCC 
CAGGAAGAGG AAGACAACAA GCTGCAGCTA AGAATGTCAG GGAAAAGGAA AAACAGGCCC 
TGCTCCTTGC CTGTGTCTGA GCTGGAGACG GTGATTGCAT CAGCCTGTGG TGAGCCTGAG 
ACCCCTCGAA CGCACTACAT TCGGATCCAC AATCTGCTTC ACAGCTTGCC CTCAGCACAG 
ATGAGTAGTG ATGGAGAGGA GGAGCCGGGA GGCGGCGAGA ATGACGAAGA GTCAACGGTC 
AAGGAAGTGT CAGAGAAGGA TGTGGTTAGC GAGTCTGAGA CGCTAGCGGC AGATCCTCCC 
CCTGAAAGCG GAACTGATGA GTATTTCAGC CGGGGGACTG TGCCCCGCAG CACCTCAGTT 
GAACACCTCT CAGACTTAAA TGGGCAGCTG TTGGATGGAG AACATCCAAC GGCTGGAAGT 
GAGAGTGAAT CAAGTTCCAG GCCATACGGG GACAATGAGT GTGACACCTC TTGCTACAGC 
CCATCCTGCT ACAGCACCTC CTGCTACAGT ACGTCCTGCT ACAGCACCTC CTGCTATAGC 
CCCTCCTGTT ATGACAGTAA CAATCGCTTT TCCAGCCATA CCCGTTTCTC CTCTGTTGAC 
AGTGCAAAGA TTTCTGAGAG CACAGTCTTT TCCTCACAGG ATGATGAGGA AGAGGAAAAC 
AGTGCCTTTG AGTCAGTGCC AGATTCAGTG CAAAGCCCTG AACTGGACAG GGAACAAGTG 
GATGTCTCCG CCCAGTGGCC AGATGAACTC ATAGCTCATG GTGGGAATTC ACAAAGAGCA 
ACAGAGATCC TAGAATCCCC AGTAGCAGGT CCAAGCAACA GAAGAGAAGG TGATTGTCCT 
TTACTCCATA ATTCTCAGCC AGTCAGCCAG CTTCCTTCCC TGAGGCCTGA CCATCATCAC 
TATCCAACTA TTGATGAGCC TCTTCCACCA AACTGGGAGG CTCGGATAGA CAGCCATGGA 
AGGGTATTTT ATGTGGATCA CGTGAATCGA ACCACAACAT GGCAGCGTCC TACAGCAGCA 
GCCACTCCAG ATGGAATACG CAGATCTGGT TCAATCCAAC AAATGGAGCA GCTCAATCGG 
CGGTATCAAA ATATTCAGCG GACTATTGCA ACAGAGAGGC CAGAGGATGA TGCTGTTGTG 
AACAACAGAG TTGAGAGGCT CTCCACTGGA GGAGGAAGTG ACTCTGAGAC AGACTCTTCC 
CAGCCAAACT CAGAAATTAG GCGAGAAGGG TCCCTTTCTC CAGTGAATTC TCAAAAAATC 
ACCTTGCTGC TACAGTCTCC AGCTGTAAAG TTTATCACAA ACCCTGAATT CTTCACCGTG 
CTGCATGCAA ACTATAGTGC CTATCGAGTC TTCACCAACA GCACCTGCTT GAAGCACATG 
ATTCTAAAGA TCCGCAGAGA TGCTCGGAAT TTTGAGCGCT ATCAGCACAA TAGGGACCTG 
GTAAATTTTA TCAATATGTT TGCTGACACC CGACTGGAAC TTCCACGGGG CTGGGAGATC 
AAAACAGACC AACAGGGCAA GTCCTTCTTT GTGGACCACA ACAGCCGTGC TACCACTTTT 
ATCGATCCTA GGATCCCACT TCAAAATGGC CGTCTCCCTA ATCATCTGAC TCACAGGCAG 
CATCTCCAGA GACTTCGCAG CTACAGTGCT GGAGAGGCAT CAGAAGTCTC CCGGAATAGA 
GGCATATCTC TGTTGACCAG ACCAGGCAAC AGCCTAGTAA CAGCTATTCG AAACCAGCAC 
CATCATGAGT CATTACCTCT AGCTTACAAT GACAAGATTG TCGCATTTCT ACGCCAACCA 
AACATTTTTG AGATGCTGCA AGAGCGTCAG CCAAGCCTGG CCAGGAACCA TGCACTCAGG 
GAGAAGATCC ATTACATTCG GACAGAGGGT ACACATGGGC TTGAAAAGCT GTCCTGTGAT 
GCAGATTTAG TAATTCTATT GAGCCTCTTT GAGGAGGATA TTATGTCCTA CATACCCCTG 
CAGGCTGCCT TCCACCCTGG CTATAGCTTC TCTCCTCGCT GTTCACCCTG CTCCTCACCT 
CAGAATTCTC CAGGATTACA GCGAGCTAGT GCACGAGCGC CCTCTCCATA TAGGAGAGAC 
TTTGAAGCCA AGCTACGCAA TTTCTATCGA AAACTTGAAG CCAAAGGATA TGGACAAGGT 
CCAGGTAAAA TTAAGTTAAT TATACGGCGT GACCACTTGT TGGAAGGCAC CTTCAATCAG 
GTGATGGCTT ATTCACGCAA GGAGCTTCAG CGGAACAAAC TCTATGTCAC ATTCGTTGGA 
GAGGAAGGCT TGGACTACAG CGGTCCCTCC CGAGAGTTCT TCTTCCTTCT GTCTCAGGAG 
CTCTTCAATC CTTACTATGG CTTGTTTGAG TATTCAGCCA ACGACACTTA CACTGTACAG 
ATCAGCCCCA TGTCTGCGTT TGTGGAAAAC CACCTTGAAT GGTTCAGGTT CAGCGGTCGT 
ATCCTTGGCC TTGCTCTCAT TCATCAGTAC CTTCTTGATG CTTTCTTCAC AAGGCCGTTC 
TATAAAGCAC TACTGAGGCT GCCGTGTGAT TTAAGTGACC TGGAGTACCT GGATGAGGAG 
TTTCACCAGA GTTTACAGTG GATGAAGGAT AACAACATCA CTGATATCCT GGATCTCACA 
TTCACCGTGA ACGAGGAGGT GTTTGGACAG GTGACAGAAA GGGAGCTGAA ATCAGGGGGT 
TCAAATACAC AGGTGACAGA AAAGAACAAG AAGGAGTACA TTGAGAGAAT GGTGAAGTGG 
CGTGTGGAAC GAGGAGTGGT TCAGCAAACA GAGGCTCTAG TCCGTGGCTT CTATGAAGTT 
GTAGACTCCA GGCTTGTCTC CATTTTTGAT GCCAGAGAGC TAGAGTTGGT GATTGCAGGT 
ACAGCAGAAA TAGACCTCAA TGACTGGCGT AACAACACAG AATATCGTGG AGGTTACCAT 
GATGGGCATA TTGTCATACG GTGGTTCTGG GCAGCAGTGG AGAGATTTAA CAATGAACAG 
AGACTACGGT TACTGCAGTT TGTCACTGGA ACATCTAGTG TACCATATGA GGGTTTTGCA 
GCCCTTCGAG GGAGCAATGG GTTACGGCGT TTCTGTATAG AAAAATGGGG GAAAATAACA 
TCTCTTCCTA GGGCGCACAC ATGTTTCAAC CGCTTGGATC TCCCGCCCTA CCCATCTTAT 
TCTATGCTGT ATGAAAAGCT GTTGACAGCT GTTGAAGAAA CTAGCACCTT TGGACTTGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_014574083.2 ) cDNA ORF clone, Pelodiscus sinensis(Chinese soft-shelled turtle) -> XP_014429569.1 Pelodiscus sinensis HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X2, mRNA.

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CloneID	OPe30299
Clone ID Related Accession (Same CDS sequence)	XM_014574083.1 , XM_025185175.1 , XM_014574083.2
Accession Version	XM_014574083.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4803bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-03
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Product	E3 ubiquitin-protein ligase HECW1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005855477.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 4, 2018 this sequence version replaced XM_014574083.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pelodiscus sinensis Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTCC ACCTGTGTAG TGTTAAGAAT CTGTACCAAA ACAGGTTCCT AGGCCTGGCT 
GCCATGGCGT CTCCATCTAG GAATTCACAG AACCGGCGCC GGTGTAAAGA ACCTCTCAGA 
TACAGCTATA ACCCTGACCA GTTTAATAAC ATGGACCTCA GGAACAATGC TCATGAGACA 
GTAACCATTC CACGATCCAC CAGTGATACT GACCTGGTCA CTTCAGACAG CCGGTCTACT 
CTCATGGTCA GCAGCTCATA TTACTCCATT GGACATTCAC AAGACCTAGT TATCTATTGG 
GATATAAAGG AAGAAGTGGA TGCAGGGGAC TGGATCGGCA TGTATCTCAT TGATGAAGTC 
TTGTCTGAAA ACTTTCTGGA CTACAAAAAC CGTGGGGTCA ATGGCTCTCA TCGTGGTCAG 
ATTATCTGGA AGATTGATGC CAACTCTTAC TTCGTAGAGT CTGAAACAAA GATCTGCTTC 
AAATACTACC ATGGAGTGAG TGGAGCACTA CGAGCTACGA CCCCCAGTGT CACAGTGAAA 
AACAGTTCTG CTCCCATTTT TAAAAGCATC GTCAATGAAG ATCCAGTCCA GGGTCAAGGA 
AGTCGGAGAC TGATCAGTTT TTCACTGTCA GATTTCCAGG CTTTGGGTCT GAAAAAAGGA 
ATGTTCTTTA ACCCAGACCC ATACCTGAAG ATTTCCATTC AGCCTGGAAA GCACAGCATC 
TTCCCAGCAC TTCCTCACCA TGGACAAGAG AAGAGATCCA AGATCACATG TAATACTGTG 
AACCCTATCT GGCAAGGAGA GCACTTCAGC TTTGTTTCCC TTCCCACGGA TGTTCTGGAA 
ATAGAAGTTA AGGACAAGTT TGCAAAGAGC CGGCCAATCA TTAAACGCTT CTTGGGGAAA 
CTTTCCATGC CAGTGCAACG CCTGCTGGAG AGACACGCAA TAGGGGACAG GGTTGTCAGC 
TACACTCTGG GACGTAGGCT TCCAACAGAT CATGTCAGCG GCCAGCTGCA GTTTCGGTTT 
GAGATAACTT CTTCTATCCA TCCAGATGAT GAAGAGGTCT CCATTAGTGC AGATCCTGAG 
TCAGCTGAAC TGCAGGAAAG CCCTGTGAAC TGCACCGCAG AGGAAATCCT GGGTGAGCCT 
CCCTGCACCG GCACAGTGAC CTCACAAAGT ACATCTCCAG AGCAGCTAAA TGGATGTCAT 
GTGAAAAGTG TCAATGTTGA TATCCCTGGG GAAGTCAAAC AGAACAGCTT AAATGAAGAG 
CTAGCAGGAA ATAGGGAGGT TGGTGTAGTG CCAGCAGATG CTACTGCGTC CTTGGAATCC 
ATCCCTGGGG AAGTGCTGCC TTCCCTGAGT GCTCTGGAAC TCTCTGAGCA GCTGGCTCTG 
CTGGTTTGCA CACCTCCTCC AGAGATTGAC TTTAGGGAAA ATAATAAAGT CAATTCTGTT 
ACTCAGGGAC ACGAGGACAT CTTCACCCGC ATGGAAGCAC TAGATACCGA TGAGGTGAGT 
GCAGACATAC AGACTGTCAA GTACTTGGAG AAAATTCAGG ATGAGGAAGG CGCTTCAGCC 
CAGGAAGAGG AAGACAACAA GCTGCAGCTA AGAATGTCAG GGAAAAGGAA AAACAGGCCC 
TGCTCCTTGC CTGTGTCTGA GCTGGAGACG GTGATTGCAT CAGCCTGTGG TGAGCCTGAG 
ACCCCTCGAA CGCACTACAT TCGGATCCAC AATCTGCTTC ACAGCTTGCC CTCAGCACAG 
ATGAGTAGTG ATGGAGAGGA GGAGCCGGGA GGCGGCGAGA ATGACGAAGA GTCAACGGTC 
AAGGAAGTGT CAGAGAAGGA TGTGGTTAGC GAGTCTGAGA CGCTAGCGGC AGATCCTCCC 
CCTGAAAGCG GAACTGATGA GTATTTCAGC CGGGGGACTG TGCCCCGCAG CACCTCAGTT 
GAACACCTCT CAGACTTAAA TGGGCAGCTG TTGGATGGAG AACATCCAAC GGCTGGAAGT 
GAGAGTGAAT CAAGTTCCAG GCCATACGGG GACAATGAGT GTGACACCTC TTGCTACAGC 
CCATCCTGCT ACAGCACCTC CTGCTACAGT ACGTCCTGCT ACAGCACCTC CTGCTATAGC 
CCCTCCTGTT ATGACAGTAA CAATCGCTTT TCCAGCCATA CCCGTTTCTC CTCTGTTGAC 
AGTGCAAAGA TTTCTGAGAG CACAGTCTTT TCCTCACAGG ATGATGAGGA AGAGGAAAAC 
AGTGCCTTTG AGTCAGTGCC AGATTCAGTG CAAAGCCCTG AACTGGACAG GGAACAAGTG 
GATGTCTCCG CCCAGTGGCC AGATGAACTC ATAGCTCATG GTGGGAATTC ACAAAGAGCA 
ACAGAGATCC TAGAATCCCC AGTAGCAGGT CCAAGCAACA GAAGAGAAGG TGATTGTCCT 
TTACTCCATA ATTCTCAGCC AGTCAGCCAG CTTCCTTCCC TGAGGCCTGA CCATCATCAC 
TATCCAACTA TTGATGAGCC TCTTCCACCA AACTGGGAGG CTCGGATAGA CAGCCATGGA 
AGGGTATTTT ATGTGGATCA CGTGAATCGA ACCACAACAT GGCAGCGTCC TACAGCAGCA 
GCCACTCCAG ATGGAATACG CAGATCTGGT TCAATCCAAC AAATGGAGCA GCTCAATCGG 
CGGTATCAAA ATATTCAGCG GACTATTGCA ACAGAGAGGC CAGAGGATGA TGCTGTTGTG 
AACAACAGAG TTGAGAGGCT CTCCACTGGA GGAGGAAGTG ACTCTGAGAC AGACTCTTCC 
CAGCCAAACT CAGAAATTAG GCGAGAAGGG TCCCTTTCTC CAGTGAATTC TCAAAAAATC 
ACCTTGCTGC TACAGTCTCC AGCTGTAAAG TTTATCACAA ACCCTGAATT CTTCACCGTG 
CTGCATGCAA ACTATAGTGC CTATCGAGTC TTCACCAACA GCACCTGCTT GAAGCACATG 
ATTCTAAAGA TCCGCAGAGA TGCTCGGAAT TTTGAGCGCT ATCAGCACAA TAGGGACCTG 
GTAAATTTTA TCAATATGTT TGCTGACACC CGACTGGAAC TTCCACGGGG CTGGGAGATC 
AAAACAGACC AACAGGGCAA GTCCTTCTTT GTGGACCACA ACAGCCGTGC TACCACTTTT 
ATCGATCCTA GGATCCCACT TCAAAATGGC CGTCTCCCTA ATCATCTGAC TCACAGGCAG 
CATCTCCAGA GACTTCGCAG CTACAGTGCT GGAGAGGCAT CAGAAGTCTC CCGGAATAGA 
GGCATATCTC TGTTGACCAG ACCAGGCAAC AGCCTAGTAA CAGCTATTCG AAACCAGCAC 
CATCATGAGT CATTACCTCT AGCTTACAAT GACAAGATTG TCGCATTTCT ACGCCAACCA 
AACATTTTTG AGATGCTGCA AGAGCGTCAG CCAAGCCTGG CCAGGAACCA TGCACTCAGG 
GAGAAGATCC ATTACATTCG GACAGAGGGT ACACATGGGC TTGAAAAGCT GTCCTGTGAT 
GCAGATTTAG TAATTCTATT GAGCCTCTTT GAGGAGGATA TTATGTCCTA CATACCCCTG 
CAGGCTGCCT TCCACCCTGG CTATAGCTTC TCTCCTCGCT GTTCACCCTG CTCCTCACCT 
CAGAATTCTC CAGGATTACA GCGAGCTAGT GCACGAGCGC CCTCTCCATA TAGGAGAGAC 
TTTGAAGCCA AGCTACGCAA TTTCTATCGA AAACTTGAAG CCAAAGGATA TGGACAAGGT 
CCAGGTAAAA TTAAGTTAAT TATACGGCGT GACCACTTGT TGGAAGGCAC CTTCAATCAG 
GTGATGGCTT ATTCACGCAA GGAGCTTCAG CGGAACAAAC TCTATGTCAC ATTCGTTGGA 
GAGGAAGGCT TGGACTACAG CGGTCCCTCC CGAGAGTTCT TCTTCCTTCT GTCTCAGGAG 
CTCTTCAATC CTTACTATGG CTTGTTTGAG TATTCAGCCA ACGACACTTA CACTGTACAG 
ATCAGCCCCA TGTCTGCGTT TGTGGAAAAC CACCTTGAAT GGTTCAGGTT CAGCGGTCGT 
ATCCTTGGCC TTGCTCTCAT TCATCAGTAC CTTCTTGATG CTTTCTTCAC AAGGCCGTTC 
TATAAAGCAC TACTGAGGCT GCCGTGTGAT TTAAGTGACC TGGAGTACCT GGATGAGGAG 
TTTCACCAGA GTTTACAGTG GATGAAGGAT AACAACATCA CTGATATCCT GGATCTCACA 
TTCACCGTGA ACGAGGAGGT GTTTGGACAG GTGACAGAAA GGGAGCTGAA ATCAGGGGGT 
TCAAATACAC AGGTGACAGA AAAGAACAAG AAGGAGTACA TTGAGAGAAT GGTGAAGTGG 
CGTGTGGAAC GAGGAGTGGT TCAGCAAACA GAGGCTCTAG TCCGTGGCTT CTATGAAGTT 
GTAGACTCCA GGCTTGTCTC CATTTTTGAT GCCAGAGAGC TAGAGTTGGT GATTGCAGGT 
ACAGCAGAAA TAGACCTCAA TGACTGGCGT AACAACACAG AATATCGTGG AGGTTACCAT 
GATGGGCATA TTGTCATACG GTGGTTCTGG GCAGCAGTGG AGAGATTTAA CAATGAACAG 
AGACTACGGT TACTGCAGTT TGTCACTGGA ACATCTAGTG TACCATATGA GGGTTTTGCA 
GCCCTTCGAG GGAGCAATGG GTTACGGCGT TTCTGTATAG AAAAATGGGG GAAAATAACA 
TCTCTTCCTA GGGCGCACAC ATGTTTCAAC CGCTTGGATC TCCCGCCCTA CCCATCTTAT 
TCTATGCTGT ATGAAAAGCT GTTGACAGCT GTTGAAGAAA CTAGCACCTT TGGACTTGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014429569.1
CDS	374..5176
Translation	MLLHLCSVKNLYQNRFLGLAAMASPSRNSQNRRRCKEPLRYSYNPDQFNNMDLRNNAHETVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIYWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDANSYFVESETKICFKYYHGVSGALRATTPSVTVKNSSAPIFKSIVNEDPVQGQGSRRLISFSLSDFQALGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQEKRSKITCNTVNPIWQGEHFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPDDEEVSISADPESAELQESPVNCTAEEILGEPPCTGTVTSQSTSPEQLNGCHVKSVNVDIPGEVKQNSLNEELAGNREVGVVPADATASLESIPGEVLPSLSALELSEQLALLVCTPPPEIDFRENNKVNSVTQGHEDIFTRMEALDTDEVSADIQTVKYLEKIQDEEGASAQEEEDNKLQLRMSGKRKNRPCSLPVSELETVIASACGEPETPRTHYIRIHNLLHSLPSAQMSSDGEEEPGGGENDEESTVKEVSEKDVVSESETLAADPPPESGTDEYFSRGTVPRSTSVEHLSDLNGQLLDGEHPTAGSESESSSRPYGDNECDTSCYSPSCYSTSCYSTSCYSTSCYSPSCYDSNNRFSSHTRFSSVDSAKISESTVFSSQDDEEEENSAFESVPDSVQSPELDREQVDVSAQWPDELIAHGGNSQRATEILESPVAGPSNRREGDCPLLHNSQPVSQLPSLRPDHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGIRRSGSIQQMEQLNRRYQNIQRTIATERPEDDAVVNNRVERLSTGGGSDSETDSSQPNSEIRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTNSTCLKHMILKIRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGISLLTRPGNSLVTAIRNQHHHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHALREKIHYIRTEGTHGLEKLSCDADLVILLSLFEEDIMSYIPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGYGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGSNTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSIFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_014574083.2
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Definition	Pelodiscus sinensis HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014574083.2

ORF Insert Sequence:

ATGCTGCTCC ACCTGTGTAG TGTTAAGAAT CTGTACCAAA ACAGGTTCCT AGGCCTGGCT 
GCCATGGCGT CTCCATCTAG GAATTCACAG AACCGGCGCC GGTGTAAAGA ACCTCTCAGA 
TACAGCTATA ACCCTGACCA GTTTAATAAC ATGGACCTCA GGAACAATGC TCATGAGACA 
GTAACCATTC CACGATCCAC CAGTGATACT GACCTGGTCA CTTCAGACAG CCGGTCTACT 
CTCATGGTCA GCAGCTCATA TTACTCCATT GGACATTCAC AAGACCTAGT TATCTATTGG 
GATATAAAGG AAGAAGTGGA TGCAGGGGAC TGGATCGGCA TGTATCTCAT TGATGAAGTC 
TTGTCTGAAA ACTTTCTGGA CTACAAAAAC CGTGGGGTCA ATGGCTCTCA TCGTGGTCAG 
ATTATCTGGA AGATTGATGC CAACTCTTAC TTCGTAGAGT CTGAAACAAA GATCTGCTTC 
AAATACTACC ATGGAGTGAG TGGAGCACTA CGAGCTACGA CCCCCAGTGT CACAGTGAAA 
AACAGTTCTG CTCCCATTTT TAAAAGCATC GTCAATGAAG ATCCAGTCCA GGGTCAAGGA 
AGTCGGAGAC TGATCAGTTT TTCACTGTCA GATTTCCAGG CTTTGGGTCT GAAAAAAGGA 
ATGTTCTTTA ACCCAGACCC ATACCTGAAG ATTTCCATTC AGCCTGGAAA GCACAGCATC 
TTCCCAGCAC TTCCTCACCA TGGACAAGAG AAGAGATCCA AGATCACATG TAATACTGTG 
AACCCTATCT GGCAAGGAGA GCACTTCAGC TTTGTTTCCC TTCCCACGGA TGTTCTGGAA 
ATAGAAGTTA AGGACAAGTT TGCAAAGAGC CGGCCAATCA TTAAACGCTT CTTGGGGAAA 
CTTTCCATGC CAGTGCAACG CCTGCTGGAG AGACACGCAA TAGGGGACAG GGTTGTCAGC 
TACACTCTGG GACGTAGGCT TCCAACAGAT CATGTCAGCG GCCAGCTGCA GTTTCGGTTT 
GAGATAACTT CTTCTATCCA TCCAGATGAT GAAGAGGTCT CCATTAGTGC AGATCCTGAG 
TCAGCTGAAC TGCAGGAAAG CCCTGTGAAC TGCACCGCAG AGGAAATCCT GGGTGAGCCT 
CCCTGCACCG GCACAGTGAC CTCACAAAGT ACATCTCCAG AGCAGCTAAA TGGATGTCAT 
GTGAAAAGTG TCAATGTTGA TATCCCTGGG GAAGTCAAAC AGAACAGCTT AAATGAAGAG 
CTAGCAGGAA ATAGGGAGGT TGGTGTAGTG CCAGCAGATG CTACTGCGTC CTTGGAATCC 
ATCCCTGGGG AAGTGCTGCC TTCCCTGAGT GCTCTGGAAC TCTCTGAGCA GCTGGCTCTG 
CTGGTTTGCA CACCTCCTCC AGAGATTGAC TTTAGGGAAA ATAATAAAGT CAATTCTGTT 
ACTCAGGGAC ACGAGGACAT CTTCACCCGC ATGGAAGCAC TAGATACCGA TGAGGTGAGT 
GCAGACATAC AGACTGTCAA GTACTTGGAG AAAATTCAGG ATGAGGAAGG CGCTTCAGCC 
CAGGAAGAGG AAGACAACAA GCTGCAGCTA AGAATGTCAG GGAAAAGGAA AAACAGGCCC 
TGCTCCTTGC CTGTGTCTGA GCTGGAGACG GTGATTGCAT CAGCCTGTGG TGAGCCTGAG 
ACCCCTCGAA CGCACTACAT TCGGATCCAC AATCTGCTTC ACAGCTTGCC CTCAGCACAG 
ATGAGTAGTG ATGGAGAGGA GGAGCCGGGA GGCGGCGAGA ATGACGAAGA GTCAACGGTC 
AAGGAAGTGT CAGAGAAGGA TGTGGTTAGC GAGTCTGAGA CGCTAGCGGC AGATCCTCCC 
CCTGAAAGCG GAACTGATGA GTATTTCAGC CGGGGGACTG TGCCCCGCAG CACCTCAGTT 
GAACACCTCT CAGACTTAAA TGGGCAGCTG TTGGATGGAG AACATCCAAC GGCTGGAAGT 
GAGAGTGAAT CAAGTTCCAG GCCATACGGG GACAATGAGT GTGACACCTC TTGCTACAGC 
CCATCCTGCT ACAGCACCTC CTGCTACAGT ACGTCCTGCT ACAGCACCTC CTGCTATAGC 
CCCTCCTGTT ATGACAGTAA CAATCGCTTT TCCAGCCATA CCCGTTTCTC CTCTGTTGAC 
AGTGCAAAGA TTTCTGAGAG CACAGTCTTT TCCTCACAGG ATGATGAGGA AGAGGAAAAC 
AGTGCCTTTG AGTCAGTGCC AGATTCAGTG CAAAGCCCTG AACTGGACAG GGAACAAGTG 
GATGTCTCCG CCCAGTGGCC AGATGAACTC ATAGCTCATG GTGGGAATTC ACAAAGAGCA 
ACAGAGATCC TAGAATCCCC AGTAGCAGGT CCAAGCAACA GAAGAGAAGG TGATTGTCCT 
TTACTCCATA ATTCTCAGCC AGTCAGCCAG CTTCCTTCCC TGAGGCCTGA CCATCATCAC 
TATCCAACTA TTGATGAGCC TCTTCCACCA AACTGGGAGG CTCGGATAGA CAGCCATGGA 
AGGGTATTTT ATGTGGATCA CGTGAATCGA ACCACAACAT GGCAGCGTCC TACAGCAGCA 
GCCACTCCAG ATGGAATACG CAGATCTGGT TCAATCCAAC AAATGGAGCA GCTCAATCGG 
CGGTATCAAA ATATTCAGCG GACTATTGCA ACAGAGAGGC CAGAGGATGA TGCTGTTGTG 
AACAACAGAG TTGAGAGGCT CTCCACTGGA GGAGGAAGTG ACTCTGAGAC AGACTCTTCC 
CAGCCAAACT CAGAAATTAG GCGAGAAGGG TCCCTTTCTC CAGTGAATTC TCAAAAAATC 
ACCTTGCTGC TACAGTCTCC AGCTGTAAAG TTTATCACAA ACCCTGAATT CTTCACCGTG 
CTGCATGCAA ACTATAGTGC CTATCGAGTC TTCACCAACA GCACCTGCTT GAAGCACATG 
ATTCTAAAGA TCCGCAGAGA TGCTCGGAAT TTTGAGCGCT ATCAGCACAA TAGGGACCTG 
GTAAATTTTA TCAATATGTT TGCTGACACC CGACTGGAAC TTCCACGGGG CTGGGAGATC 
AAAACAGACC AACAGGGCAA GTCCTTCTTT GTGGACCACA ACAGCCGTGC TACCACTTTT 
ATCGATCCTA GGATCCCACT TCAAAATGGC CGTCTCCCTA ATCATCTGAC TCACAGGCAG 
CATCTCCAGA GACTTCGCAG CTACAGTGCT GGAGAGGCAT CAGAAGTCTC CCGGAATAGA 
GGCATATCTC TGTTGACCAG ACCAGGCAAC AGCCTAGTAA CAGCTATTCG AAACCAGCAC 
CATCATGAGT CATTACCTCT AGCTTACAAT GACAAGATTG TCGCATTTCT ACGCCAACCA 
AACATTTTTG AGATGCTGCA AGAGCGTCAG CCAAGCCTGG CCAGGAACCA TGCACTCAGG 
GAGAAGATCC ATTACATTCG GACAGAGGGT ACACATGGGC TTGAAAAGCT GTCCTGTGAT 
GCAGATTTAG TAATTCTATT GAGCCTCTTT GAGGAGGATA TTATGTCCTA CATACCCCTG 
CAGGCTGCCT TCCACCCTGG CTATAGCTTC TCTCCTCGCT GTTCACCCTG CTCCTCACCT 
CAGAATTCTC CAGGATTACA GCGAGCTAGT GCACGAGCGC CCTCTCCATA TAGGAGAGAC 
TTTGAAGCCA AGCTACGCAA TTTCTATCGA AAACTTGAAG CCAAAGGATA TGGACAAGGT 
CCAGGTAAAA TTAAGTTAAT TATACGGCGT GACCACTTGT TGGAAGGCAC CTTCAATCAG 
GTGATGGCTT ATTCACGCAA GGAGCTTCAG CGGAACAAAC TCTATGTCAC ATTCGTTGGA 
GAGGAAGGCT TGGACTACAG CGGTCCCTCC CGAGAGTTCT TCTTCCTTCT GTCTCAGGAG 
CTCTTCAATC CTTACTATGG CTTGTTTGAG TATTCAGCCA ACGACACTTA CACTGTACAG 
ATCAGCCCCA TGTCTGCGTT TGTGGAAAAC CACCTTGAAT GGTTCAGGTT CAGCGGTCGT 
ATCCTTGGCC TTGCTCTCAT TCATCAGTAC CTTCTTGATG CTTTCTTCAC AAGGCCGTTC 
TATAAAGCAC TACTGAGGCT GCCGTGTGAT TTAAGTGACC TGGAGTACCT GGATGAGGAG 
TTTCACCAGA GTTTACAGTG GATGAAGGAT AACAACATCA CTGATATCCT GGATCTCACA 
TTCACCGTGA ACGAGGAGGT GTTTGGACAG GTGACAGAAA GGGAGCTGAA ATCAGGGGGT 
TCAAATACAC AGGTGACAGA AAAGAACAAG AAGGAGTACA TTGAGAGAAT GGTGAAGTGG 
CGTGTGGAAC GAGGAGTGGT TCAGCAAACA GAGGCTCTAG TCCGTGGCTT CTATGAAGTT 
GTAGACTCCA GGCTTGTCTC CATTTTTGAT GCCAGAGAGC TAGAGTTGGT GATTGCAGGT 
ACAGCAGAAA TAGACCTCAA TGACTGGCGT AACAACACAG AATATCGTGG AGGTTACCAT 
GATGGGCATA TTGTCATACG GTGGTTCTGG GCAGCAGTGG AGAGATTTAA CAATGAACAG 
AGACTACGGT TACTGCAGTT TGTCACTGGA ACATCTAGTG TACCATATGA GGGTTTTGCA 
GCCCTTCGAG GGAGCAATGG GTTACGGCGT TTCTGTATAG AAAAATGGGG GAAAATAACA 
TCTCTTCCTA GGGCGCACAC ATGTTTCAAC CGCTTGGATC TCCCGCCCTA CCCATCTTAT 
TCTATGCTGT ATGAAAAGCT GTTGACAGCT GTTGAAGAAA CTAGCACCTT TGGACTTGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_006124096.3 ) cDNA ORF clone, Pelodiscus sinensis(Chinese soft-shelled turtle) -> XP_006124158.1 Pelodiscus sinensis HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OPe30300
Clone ID Related Accession (Same CDS sequence)	XM_006124096.3 , XM_006124096.2
Accession Version	XM_006124096.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4740bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-03
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Product	E3 ubiquitin-protein ligase HECW1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005855477.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 4, 2018 this sequence version replaced XM_006124096.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pelodiscus sinensis Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCTC CATCTAGGAA TTCACAGAAC CGGCGCCGGT GTAAAGAACC TCTCAGATAC 
AGCTATAACC CTGACCAGTT TAATAACATG GACCTCAGGA ACAATGCTCA TGAGACAGTA 
ACCATTCCAC GATCCACCAG TGATACTGAC CTGGTCACTT CAGACAGCCG GTCTACTCTC 
ATGGTCAGCA GCTCATATTA CTCCATTGGA CATTCACAAG ACCTAGTTAT CTATTGGGAT 
ATAAAGGAAG AAGTGGATGC AGGGGACTGG ATCGGCATGT ATCTCATTGA TGAAGTCTTG 
TCTGAAAACT TTCTGGACTA CAAAAACCGT GGGGTCAATG GCTCTCATCG TGGTCAGATT 
ATCTGGAAGA TTGATGCCAA CTCTTACTTC GTAGAGTCTG AAACAAAGAT CTGCTTCAAA 
TACTACCATG GAGTGAGTGG AGCACTACGA GCTACGACCC CCAGTGTCAC AGTGAAAAAC 
AGTTCTGCTC CCATTTTTAA AAGCATCGTC AATGAAGATC CAGTCCAGGG TCAAGGAAGT 
CGGAGACTGA TCAGTTTTTC ACTGTCAGAT TTCCAGGCTT TGGGTCTGAA AAAAGGAATG 
TTCTTTAACC CAGACCCATA CCTGAAGATT TCCATTCAGC CTGGAAAGCA CAGCATCTTC 
CCAGCACTTC CTCACCATGG ACAAGAGAAG AGATCCAAGA TCACATGTAA TACTGTGAAC 
CCTATCTGGC AAGGAGAGCA CTTCAGCTTT GTTTCCCTTC CCACGGATGT TCTGGAAATA 
GAAGTTAAGG ACAAGTTTGC AAAGAGCCGG CCAATCATTA AACGCTTCTT GGGGAAACTT 
TCCATGCCAG TGCAACGCCT GCTGGAGAGA CACGCAATAG GGGACAGGGT TGTCAGCTAC 
ACTCTGGGAC GTAGGCTTCC AACAGATCAT GTCAGCGGCC AGCTGCAGTT TCGGTTTGAG 
ATAACTTCTT CTATCCATCC AGATGATGAA GAGGTCTCCA TTAGTGCAGA TCCTGAGTCA 
GCTGAACTGC AGGAAAGCCC TGTGAACTGC ACCGCAGAGG AAATCCTGGG TGAGCCTCCC 
TGCACCGGCA CAGTGACCTC ACAAAGTACA TCTCCAGAGC AGCTAAATGG ATGTCATGTG 
AAAAGTGTCA ATGTTGATAT CCCTGGGGAA GTCAAACAGA ACAGCTTAAA TGAAGAGCTA 
GCAGGAAATA GGGAGGTTGG TGTAGTGCCA GCAGATGCTA CTGCGTCCTT GGAATCCATC 
CCTGGGGAAG TGCTGCCTTC CCTGAGTGCT CTGGAACTCT CTGAGCAGCT GGCTCTGCTG 
GTTTGCACAC CTCCTCCAGA GATTGACTTT AGGGAAAATA ATAAAGTCAA TTCTGTTACT 
CAGGGACACG AGGACATCTT CACCCGCATG GAAGCACTAG ATACCGATGA GGTGAGTGCA 
GACATACAGA CTGTCAAGTA CTTGGAGAAA ATTCAGGATG AGGAAGGCGC TTCAGCCCAG 
GAAGAGGAAG ACAACAAGCT GCAGCTAAGA ATGTCAGGGA AAAGGAAAAA CAGGCCCTGC 
TCCTTGCCTG TGTCTGAGCT GGAGACGGTG ATTGCATCAG CCTGTGGTGA GCCTGAGACC 
CCTCGAACGC ACTACATTCG GATCCACAAT CTGCTTCACA GCTTGCCCTC AGCACAGATG 
AGTAGTGATG GAGAGGAGGA GCCGGGAGGC GGCGAGAATG ACGAAGAGTC AACGGTCAAG 
GAAGTGTCAG AGAAGGATGT GGTTAGCGAG TCTGAGACGC TAGCGGCAGA TCCTCCCCCT 
GAAAGCGGAA CTGATGAGTA TTTCAGCCGG GGGACTGTGC CCCGCAGCAC CTCAGTTGAA 
CACCTCTCAG ACTTAAATGG GCAGCTGTTG GATGGAGAAC ATCCAACGGC TGGAAGTGAG 
AGTGAATCAA GTTCCAGGCC ATACGGGGAC AATGAGTGTG ACACCTCTTG CTACAGCCCA 
TCCTGCTACA GCACCTCCTG CTACAGTACG TCCTGCTACA GCACCTCCTG CTATAGCCCC 
TCCTGTTATG ACAGTAACAA TCGCTTTTCC AGCCATACCC GTTTCTCCTC TGTTGACAGT 
GCAAAGATTT CTGAGAGCAC AGTCTTTTCC TCACAGGATG ATGAGGAAGA GGAAAACAGT 
GCCTTTGAGT CAGTGCCAGA TTCAGTGCAA AGCCCTGAAC TGGACAGGGA ACAAGTGGAT 
GTCTCCGCCC AGTGGCCAGA TGAACTCATA GCTCATGGTG GGAATTCACA AAGAGCAACA 
GAGATCCTAG AATCCCCAGT AGCAGGTCCA AGCAACAGAA GAGAAGGTGA TTGTCCTTTA 
CTCCATAATT CTCAGCCAGT CAGCCAGCTT CCTTCCCTGA GGCCTGACCA TCATCACTAT 
CCAACTATTG ATGAGCCTCT TCCACCAAAC TGGGAGGCTC GGATAGACAG CCATGGAAGG 
GTATTTTATG TGGATCACGT GAATCGAACC ACAACATGGC AGCGTCCTAC AGCAGCAGCC 
ACTCCAGATG GAATACGCAG ATCTGGTTCA ATCCAACAAA TGGAGCAGCT CAATCGGCGG 
TATCAAAATA TTCAGCGGAC TATTGCAACA GAGAGGCCAG AGGATGATGC TGTTGTGAAC 
AACAGAGTTG AGAGGCTCTC CACTGGAGGA GGAAGTGACT CTGAGACAGA CTCTTCCCAG 
CCAAACTCAG AAATTAGGCG AGAAGGGTCC CTTTCTCCAG TGAATTCTCA AAAAATCACC 
TTGCTGCTAC AGTCTCCAGC TGTAAAGTTT ATCACAAACC CTGAATTCTT CACCGTGCTG 
CATGCAAACT ATAGTGCCTA TCGAGTCTTC ACCAACAGCA CCTGCTTGAA GCACATGATT 
CTAAAGATCC GCAGAGATGC TCGGAATTTT GAGCGCTATC AGCACAATAG GGACCTGGTA 
AATTTTATCA ATATGTTTGC TGACACCCGA CTGGAACTTC CACGGGGCTG GGAGATCAAA 
ACAGACCAAC AGGGCAAGTC CTTCTTTGTG GACCACAACA GCCGTGCTAC CACTTTTATC 
GATCCTAGGA TCCCACTTCA AAATGGCCGT CTCCCTAATC ATCTGACTCA CAGGCAGCAT 
CTCCAGAGAC TTCGCAGCTA CAGTGCTGGA GAGGCATCAG AAGTCTCCCG GAATAGAGGC 
ATATCTCTGT TGACCAGACC AGGCAACAGC CTAGTAACAG CTATTCGAAA CCAGCACCAT 
CATGAGTCAT TACCTCTAGC TTACAATGAC AAGATTGTCG CATTTCTACG CCAACCAAAC 
ATTTTTGAGA TGCTGCAAGA GCGTCAGCCA AGCCTGGCCA GGAACCATGC ACTCAGGGAG 
AAGATCCATT ACATTCGGAC AGAGGGTACA CATGGGCTTG AAAAGCTGTC CTGTGATGCA 
GATTTAGTAA TTCTATTGAG CCTCTTTGAG GAGGATATTA TGTCCTACAT ACCCCTGCAG 
GCTGCCTTCC ACCCTGGCTA TAGCTTCTCT CCTCGCTGTT CACCCTGCTC CTCACCTCAG 
AATTCTCCAG GATTACAGCG AGCTAGTGCA CGAGCGCCCT CTCCATATAG GAGAGACTTT 
GAAGCCAAGC TACGCAATTT CTATCGAAAA CTTGAAGCCA AAGGATATGG ACAAGGTCCA 
GGTAAAATTA AGTTAATTAT ACGGCGTGAC CACTTGTTGG AAGGCACCTT CAATCAGGTG 
ATGGCTTATT CACGCAAGGA GCTTCAGCGG AACAAACTCT ATGTCACATT CGTTGGAGAG 
GAAGGCTTGG ACTACAGCGG TCCCTCCCGA GAGTTCTTCT TCCTTCTGTC TCAGGAGCTC 
TTCAATCCTT ACTATGGCTT GTTTGAGTAT TCAGCCAACG ACACTTACAC TGTACAGATC 
AGCCCCATGT CTGCGTTTGT GGAAAACCAC CTTGAATGGT TCAGGTTCAG CGGTCGTATC 
CTTGGCCTTG CTCTCATTCA TCAGTACCTT CTTGATGCTT TCTTCACAAG GCCGTTCTAT 
AAAGCACTAC TGAGGCTGCC GTGTGATTTA AGTGACCTGG AGTACCTGGA TGAGGAGTTT 
CACCAGAGTT TACAGTGGAT GAAGGATAAC AACATCACTG ATATCCTGGA TCTCACATTC 
ACCGTGAACG AGGAGGTGTT TGGACAGGTG ACAGAAAGGG AGCTGAAATC AGGGGGTTCA 
AATACACAGG TGACAGAAAA GAACAAGAAG GAGTACATTG AGAGAATGGT GAAGTGGCGT 
GTGGAACGAG GAGTGGTTCA GCAAACAGAG GCTCTAGTCC GTGGCTTCTA TGAAGTTGTA 
GACTCCAGGC TTGTCTCCAT TTTTGATGCC AGAGAGCTAG AGTTGGTGAT TGCAGGTACA 
GCAGAAATAG ACCTCAATGA CTGGCGTAAC AACACAGAAT ATCGTGGAGG TTACCATGAT 
GGGCATATTG TCATACGGTG GTTCTGGGCA GCAGTGGAGA GATTTAACAA TGAACAGAGA 
CTACGGTTAC TGCAGTTTGT CACTGGAACA TCTAGTGTAC CATATGAGGG TTTTGCAGCC 
CTTCGAGGGA GCAATGGGTT ACGGCGTTTC TGTATAGAAA AATGGGGGAA AATAACATCT 
CTTCCTAGGG CGCACACATG TTTCAACCGC TTGGATCTCC CGCCCTACCC ATCTTATTCT 
ATGCTGTATG AAAAGCTGTT GACAGCTGTT GAAGAAACTA GCACCTTTGG ACTTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006124158.1
CDS	379..5118
Translation	MASPSRNSQNRRRCKEPLRYSYNPDQFNNMDLRNNAHETVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIYWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDANSYFVESETKICFKYYHGVSGALRATTPSVTVKNSSAPIFKSIVNEDPVQGQGSRRLISFSLSDFQALGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQEKRSKITCNTVNPIWQGEHFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPDDEEVSISADPESAELQESPVNCTAEEILGEPPCTGTVTSQSTSPEQLNGCHVKSVNVDIPGEVKQNSLNEELAGNREVGVVPADATASLESIPGEVLPSLSALELSEQLALLVCTPPPEIDFRENNKVNSVTQGHEDIFTRMEALDTDEVSADIQTVKYLEKIQDEEGASAQEEEDNKLQLRMSGKRKNRPCSLPVSELETVIASACGEPETPRTHYIRIHNLLHSLPSAQMSSDGEEEPGGGENDEESTVKEVSEKDVVSESETLAADPPPESGTDEYFSRGTVPRSTSVEHLSDLNGQLLDGEHPTAGSESESSSRPYGDNECDTSCYSPSCYSTSCYSTSCYSTSCYSPSCYDSNNRFSSHTRFSSVDSAKISESTVFSSQDDEEEENSAFESVPDSVQSPELDREQVDVSAQWPDELIAHGGNSQRATEILESPVAGPSNRREGDCPLLHNSQPVSQLPSLRPDHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGIRRSGSIQQMEQLNRRYQNIQRTIATERPEDDAVVNNRVERLSTGGGSDSETDSSQPNSEIRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTNSTCLKHMILKIRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGISLLTRPGNSLVTAIRNQHHHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHALREKIHYIRTEGTHGLEKLSCDADLVILLSLFEEDIMSYIPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGYGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGSNTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSIFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_006124096.3
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Definition	Pelodiscus sinensis HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006124096.3

ORF Insert Sequence:

ATGGCGTCTC CATCTAGGAA TTCACAGAAC CGGCGCCGGT GTAAAGAACC TCTCAGATAC 
AGCTATAACC CTGACCAGTT TAATAACATG GACCTCAGGA ACAATGCTCA TGAGACAGTA 
ACCATTCCAC GATCCACCAG TGATACTGAC CTGGTCACTT CAGACAGCCG GTCTACTCTC 
ATGGTCAGCA GCTCATATTA CTCCATTGGA CATTCACAAG ACCTAGTTAT CTATTGGGAT 
ATAAAGGAAG AAGTGGATGC AGGGGACTGG ATCGGCATGT ATCTCATTGA TGAAGTCTTG 
TCTGAAAACT TTCTGGACTA CAAAAACCGT GGGGTCAATG GCTCTCATCG TGGTCAGATT 
ATCTGGAAGA TTGATGCCAA CTCTTACTTC GTAGAGTCTG AAACAAAGAT CTGCTTCAAA 
TACTACCATG GAGTGAGTGG AGCACTACGA GCTACGACCC CCAGTGTCAC AGTGAAAAAC 
AGTTCTGCTC CCATTTTTAA AAGCATCGTC AATGAAGATC CAGTCCAGGG TCAAGGAAGT 
CGGAGACTGA TCAGTTTTTC ACTGTCAGAT TTCCAGGCTT TGGGTCTGAA AAAAGGAATG 
TTCTTTAACC CAGACCCATA CCTGAAGATT TCCATTCAGC CTGGAAAGCA CAGCATCTTC 
CCAGCACTTC CTCACCATGG ACAAGAGAAG AGATCCAAGA TCACATGTAA TACTGTGAAC 
CCTATCTGGC AAGGAGAGCA CTTCAGCTTT GTTTCCCTTC CCACGGATGT TCTGGAAATA 
GAAGTTAAGG ACAAGTTTGC AAAGAGCCGG CCAATCATTA AACGCTTCTT GGGGAAACTT 
TCCATGCCAG TGCAACGCCT GCTGGAGAGA CACGCAATAG GGGACAGGGT TGTCAGCTAC 
ACTCTGGGAC GTAGGCTTCC AACAGATCAT GTCAGCGGCC AGCTGCAGTT TCGGTTTGAG 
ATAACTTCTT CTATCCATCC AGATGATGAA GAGGTCTCCA TTAGTGCAGA TCCTGAGTCA 
GCTGAACTGC AGGAAAGCCC TGTGAACTGC ACCGCAGAGG AAATCCTGGG TGAGCCTCCC 
TGCACCGGCA CAGTGACCTC ACAAAGTACA TCTCCAGAGC AGCTAAATGG ATGTCATGTG 
AAAAGTGTCA ATGTTGATAT CCCTGGGGAA GTCAAACAGA ACAGCTTAAA TGAAGAGCTA 
GCAGGAAATA GGGAGGTTGG TGTAGTGCCA GCAGATGCTA CTGCGTCCTT GGAATCCATC 
CCTGGGGAAG TGCTGCCTTC CCTGAGTGCT CTGGAACTCT CTGAGCAGCT GGCTCTGCTG 
GTTTGCACAC CTCCTCCAGA GATTGACTTT AGGGAAAATA ATAAAGTCAA TTCTGTTACT 
CAGGGACACG AGGACATCTT CACCCGCATG GAAGCACTAG ATACCGATGA GGTGAGTGCA 
GACATACAGA CTGTCAAGTA CTTGGAGAAA ATTCAGGATG AGGAAGGCGC TTCAGCCCAG 
GAAGAGGAAG ACAACAAGCT GCAGCTAAGA ATGTCAGGGA AAAGGAAAAA CAGGCCCTGC 
TCCTTGCCTG TGTCTGAGCT GGAGACGGTG ATTGCATCAG CCTGTGGTGA GCCTGAGACC 
CCTCGAACGC ACTACATTCG GATCCACAAT CTGCTTCACA GCTTGCCCTC AGCACAGATG 
AGTAGTGATG GAGAGGAGGA GCCGGGAGGC GGCGAGAATG ACGAAGAGTC AACGGTCAAG 
GAAGTGTCAG AGAAGGATGT GGTTAGCGAG TCTGAGACGC TAGCGGCAGA TCCTCCCCCT 
GAAAGCGGAA CTGATGAGTA TTTCAGCCGG GGGACTGTGC CCCGCAGCAC CTCAGTTGAA 
CACCTCTCAG ACTTAAATGG GCAGCTGTTG GATGGAGAAC ATCCAACGGC TGGAAGTGAG 
AGTGAATCAA GTTCCAGGCC ATACGGGGAC AATGAGTGTG ACACCTCTTG CTACAGCCCA 
TCCTGCTACA GCACCTCCTG CTACAGTACG TCCTGCTACA GCACCTCCTG CTATAGCCCC 
TCCTGTTATG ACAGTAACAA TCGCTTTTCC AGCCATACCC GTTTCTCCTC TGTTGACAGT 
GCAAAGATTT CTGAGAGCAC AGTCTTTTCC TCACAGGATG ATGAGGAAGA GGAAAACAGT 
GCCTTTGAGT CAGTGCCAGA TTCAGTGCAA AGCCCTGAAC TGGACAGGGA ACAAGTGGAT 
GTCTCCGCCC AGTGGCCAGA TGAACTCATA GCTCATGGTG GGAATTCACA AAGAGCAACA 
GAGATCCTAG AATCCCCAGT AGCAGGTCCA AGCAACAGAA GAGAAGGTGA TTGTCCTTTA 
CTCCATAATT CTCAGCCAGT CAGCCAGCTT CCTTCCCTGA GGCCTGACCA TCATCACTAT 
CCAACTATTG ATGAGCCTCT TCCACCAAAC TGGGAGGCTC GGATAGACAG CCATGGAAGG 
GTATTTTATG TGGATCACGT GAATCGAACC ACAACATGGC AGCGTCCTAC AGCAGCAGCC 
ACTCCAGATG GAATACGCAG ATCTGGTTCA ATCCAACAAA TGGAGCAGCT CAATCGGCGG 
TATCAAAATA TTCAGCGGAC TATTGCAACA GAGAGGCCAG AGGATGATGC TGTTGTGAAC 
AACAGAGTTG AGAGGCTCTC CACTGGAGGA GGAAGTGACT CTGAGACAGA CTCTTCCCAG 
CCAAACTCAG AAATTAGGCG AGAAGGGTCC CTTTCTCCAG TGAATTCTCA AAAAATCACC 
TTGCTGCTAC AGTCTCCAGC TGTAAAGTTT ATCACAAACC CTGAATTCTT CACCGTGCTG 
CATGCAAACT ATAGTGCCTA TCGAGTCTTC ACCAACAGCA CCTGCTTGAA GCACATGATT 
CTAAAGATCC GCAGAGATGC TCGGAATTTT GAGCGCTATC AGCACAATAG GGACCTGGTA 
AATTTTATCA ATATGTTTGC TGACACCCGA CTGGAACTTC CACGGGGCTG GGAGATCAAA 
ACAGACCAAC AGGGCAAGTC CTTCTTTGTG GACCACAACA GCCGTGCTAC CACTTTTATC 
GATCCTAGGA TCCCACTTCA AAATGGCCGT CTCCCTAATC ATCTGACTCA CAGGCAGCAT 
CTCCAGAGAC TTCGCAGCTA CAGTGCTGGA GAGGCATCAG AAGTCTCCCG GAATAGAGGC 
ATATCTCTGT TGACCAGACC AGGCAACAGC CTAGTAACAG CTATTCGAAA CCAGCACCAT 
CATGAGTCAT TACCTCTAGC TTACAATGAC AAGATTGTCG CATTTCTACG CCAACCAAAC 
ATTTTTGAGA TGCTGCAAGA GCGTCAGCCA AGCCTGGCCA GGAACCATGC ACTCAGGGAG 
AAGATCCATT ACATTCGGAC AGAGGGTACA CATGGGCTTG AAAAGCTGTC CTGTGATGCA 
GATTTAGTAA TTCTATTGAG CCTCTTTGAG GAGGATATTA TGTCCTACAT ACCCCTGCAG 
GCTGCCTTCC ACCCTGGCTA TAGCTTCTCT CCTCGCTGTT CACCCTGCTC CTCACCTCAG 
AATTCTCCAG GATTACAGCG AGCTAGTGCA CGAGCGCCCT CTCCATATAG GAGAGACTTT 
GAAGCCAAGC TACGCAATTT CTATCGAAAA CTTGAAGCCA AAGGATATGG ACAAGGTCCA 
GGTAAAATTA AGTTAATTAT ACGGCGTGAC CACTTGTTGG AAGGCACCTT CAATCAGGTG 
ATGGCTTATT CACGCAAGGA GCTTCAGCGG AACAAACTCT ATGTCACATT CGTTGGAGAG 
GAAGGCTTGG ACTACAGCGG TCCCTCCCGA GAGTTCTTCT TCCTTCTGTC TCAGGAGCTC 
TTCAATCCTT ACTATGGCTT GTTTGAGTAT TCAGCCAACG ACACTTACAC TGTACAGATC 
AGCCCCATGT CTGCGTTTGT GGAAAACCAC CTTGAATGGT TCAGGTTCAG CGGTCGTATC 
CTTGGCCTTG CTCTCATTCA TCAGTACCTT CTTGATGCTT TCTTCACAAG GCCGTTCTAT 
AAAGCACTAC TGAGGCTGCC GTGTGATTTA AGTGACCTGG AGTACCTGGA TGAGGAGTTT 
CACCAGAGTT TACAGTGGAT GAAGGATAAC AACATCACTG ATATCCTGGA TCTCACATTC 
ACCGTGAACG AGGAGGTGTT TGGACAGGTG ACAGAAAGGG AGCTGAAATC AGGGGGTTCA 
AATACACAGG TGACAGAAAA GAACAAGAAG GAGTACATTG AGAGAATGGT GAAGTGGCGT 
GTGGAACGAG GAGTGGTTCA GCAAACAGAG GCTCTAGTCC GTGGCTTCTA TGAAGTTGTA 
GACTCCAGGC TTGTCTCCAT TTTTGATGCC AGAGAGCTAG AGTTGGTGAT TGCAGGTACA 
GCAGAAATAG ACCTCAATGA CTGGCGTAAC AACACAGAAT ATCGTGGAGG TTACCATGAT 
GGGCATATTG TCATACGGTG GTTCTGGGCA GCAGTGGAGA GATTTAACAA TGAACAGAGA 
CTACGGTTAC TGCAGTTTGT CACTGGAACA TCTAGTGTAC CATATGAGGG TTTTGCAGCC 
CTTCGAGGGA GCAATGGGTT ACGGCGTTTC TGTATAGAAA AATGGGGGAA AATAACATCT 
CTTCCTAGGG CGCACACATG TTTCAACCGC TTGGATCTCC CGCCCTACCC ATCTTATTCT 
ATGCTGTATG AAAAGCTGTT GACAGCTGTT GAAGAAACTA GCACCTTTGG ACTTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_006124096.2 ) cDNA ORF clone, Pelodiscus sinensis(Chinese soft-shelled turtle) -> XP_006124158.1 Pelodiscus sinensis HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPe30300
Clone ID Related Accession (Same CDS sequence)	XM_006124096.3 , XM_006124096.2
Accession Version	XM_006124096.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4740bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-11-02
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Product	E3 ubiquitin-protein ligase HECW1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005855477.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 3, 2015 this sequence version replaced XM_006124096.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pelodiscus sinensis Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCTC CATCTAGGAA TTCACAGAAC CGGCGCCGGT GTAAAGAACC TCTCAGATAC 
AGCTATAACC CTGACCAGTT TAATAACATG GACCTCAGGA ACAATGCTCA TGAGACAGTA 
ACCATTCCAC GATCCACCAG TGATACTGAC CTGGTCACTT CAGACAGCCG GTCTACTCTC 
ATGGTCAGCA GCTCATATTA CTCCATTGGA CATTCACAAG ACCTAGTTAT CTATTGGGAT 
ATAAAGGAAG AAGTGGATGC AGGGGACTGG ATCGGCATGT ATCTCATTGA TGAAGTCTTG 
TCTGAAAACT TTCTGGACTA CAAAAACCGT GGGGTCAATG GCTCTCATCG TGGTCAGATT 
ATCTGGAAGA TTGATGCCAA CTCTTACTTC GTAGAGTCTG AAACAAAGAT CTGCTTCAAA 
TACTACCATG GAGTGAGTGG AGCACTACGA GCTACGACCC CCAGTGTCAC AGTGAAAAAC 
AGTTCTGCTC CCATTTTTAA AAGCATCGTC AATGAAGATC CAGTCCAGGG TCAAGGAAGT 
CGGAGACTGA TCAGTTTTTC ACTGTCAGAT TTCCAGGCTT TGGGTCTGAA AAAAGGAATG 
TTCTTTAACC CAGACCCATA CCTGAAGATT TCCATTCAGC CTGGAAAGCA CAGCATCTTC 
CCAGCACTTC CTCACCATGG ACAAGAGAAG AGATCCAAGA TCACATGTAA TACTGTGAAC 
CCTATCTGGC AAGGAGAGCA CTTCAGCTTT GTTTCCCTTC CCACGGATGT TCTGGAAATA 
GAAGTTAAGG ACAAGTTTGC AAAGAGCCGG CCAATCATTA AACGCTTCTT GGGGAAACTT 
TCCATGCCAG TGCAACGCCT GCTGGAGAGA CACGCAATAG GGGACAGGGT TGTCAGCTAC 
ACTCTGGGAC GTAGGCTTCC AACAGATCAT GTCAGCGGCC AGCTGCAGTT TCGGTTTGAG 
ATAACTTCTT CTATCCATCC AGATGATGAA GAGGTCTCCA TTAGTGCAGA TCCTGAGTCA 
GCTGAACTGC AGGAAAGCCC TGTGAACTGC ACCGCAGAGG AAATCCTGGG TGAGCCTCCC 
TGCACCGGCA CAGTGACCTC ACAAAGTACA TCTCCAGAGC AGCTAAATGG ATGTCATGTG 
AAAAGTGTCA ATGTTGATAT CCCTGGGGAA GTCAAACAGA ACAGCTTAAA TGAAGAGCTA 
GCAGGAAATA GGGAGGTTGG TGTAGTGCCA GCAGATGCTA CTGCGTCCTT GGAATCCATC 
CCTGGGGAAG TGCTGCCTTC CCTGAGTGCT CTGGAACTCT CTGAGCAGCT GGCTCTGCTG 
GTTTGCACAC CTCCTCCAGA GATTGACTTT AGGGAAAATA ATAAAGTCAA TTCTGTTACT 
CAGGGACACG AGGACATCTT CACCCGCATG GAAGCACTAG ATACCGATGA GGTGAGTGCA 
GACATACAGA CTGTCAAGTA CTTGGAGAAA ATTCAGGATG AGGAAGGCGC TTCAGCCCAG 
GAAGAGGAAG ACAACAAGCT GCAGCTAAGA ATGTCAGGGA AAAGGAAAAA CAGGCCCTGC 
TCCTTGCCTG TGTCTGAGCT GGAGACGGTG ATTGCATCAG CCTGTGGTGA GCCTGAGACC 
CCTCGAACGC ACTACATTCG GATCCACAAT CTGCTTCACA GCTTGCCCTC AGCACAGATG 
AGTAGTGATG GAGAGGAGGA GCCGGGAGGC GGCGAGAATG ACGAAGAGTC AACGGTCAAG 
GAAGTGTCAG AGAAGGATGT GGTTAGCGAG TCTGAGACGC TAGCGGCAGA TCCTCCCCCT 
GAAAGCGGAA CTGATGAGTA TTTCAGCCGG GGGACTGTGC CCCGCAGCAC CTCAGTTGAA 
CACCTCTCAG ACTTAAATGG GCAGCTGTTG GATGGAGAAC ATCCAACGGC TGGAAGTGAG 
AGTGAATCAA GTTCCAGGCC ATACGGGGAC AATGAGTGTG ACACCTCTTG CTACAGCCCA 
TCCTGCTACA GCACCTCCTG CTACAGTACG TCCTGCTACA GCACCTCCTG CTATAGCCCC 
TCCTGTTATG ACAGTAACAA TCGCTTTTCC AGCCATACCC GTTTCTCCTC TGTTGACAGT 
GCAAAGATTT CTGAGAGCAC AGTCTTTTCC TCACAGGATG ATGAGGAAGA GGAAAACAGT 
GCCTTTGAGT CAGTGCCAGA TTCAGTGCAA AGCCCTGAAC TGGACAGGGA ACAAGTGGAT 
GTCTCCGCCC AGTGGCCAGA TGAACTCATA GCTCATGGTG GGAATTCACA AAGAGCAACA 
GAGATCCTAG AATCCCCAGT AGCAGGTCCA AGCAACAGAA GAGAAGGTGA TTGTCCTTTA 
CTCCATAATT CTCAGCCAGT CAGCCAGCTT CCTTCCCTGA GGCCTGACCA TCATCACTAT 
CCAACTATTG ATGAGCCTCT TCCACCAAAC TGGGAGGCTC GGATAGACAG CCATGGAAGG 
GTATTTTATG TGGATCACGT GAATCGAACC ACAACATGGC AGCGTCCTAC AGCAGCAGCC 
ACTCCAGATG GAATACGCAG ATCTGGTTCA ATCCAACAAA TGGAGCAGCT CAATCGGCGG 
TATCAAAATA TTCAGCGGAC TATTGCAACA GAGAGGCCAG AGGATGATGC TGTTGTGAAC 
AACAGAGTTG AGAGGCTCTC CACTGGAGGA GGAAGTGACT CTGAGACAGA CTCTTCCCAG 
CCAAACTCAG AAATTAGGCG AGAAGGGTCC CTTTCTCCAG TGAATTCTCA AAAAATCACC 
TTGCTGCTAC AGTCTCCAGC TGTAAAGTTT ATCACAAACC CTGAATTCTT CACCGTGCTG 
CATGCAAACT ATAGTGCCTA TCGAGTCTTC ACCAACAGCA CCTGCTTGAA GCACATGATT 
CTAAAGATCC GCAGAGATGC TCGGAATTTT GAGCGCTATC AGCACAATAG GGACCTGGTA 
AATTTTATCA ATATGTTTGC TGACACCCGA CTGGAACTTC CACGGGGCTG GGAGATCAAA 
ACAGACCAAC AGGGCAAGTC CTTCTTTGTG GACCACAACA GCCGTGCTAC CACTTTTATC 
GATCCTAGGA TCCCACTTCA AAATGGCCGT CTCCCTAATC ATCTGACTCA CAGGCAGCAT 
CTCCAGAGAC TTCGCAGCTA CAGTGCTGGA GAGGCATCAG AAGTCTCCCG GAATAGAGGC 
ATATCTCTGT TGACCAGACC AGGCAACAGC CTAGTAACAG CTATTCGAAA CCAGCACCAT 
CATGAGTCAT TACCTCTAGC TTACAATGAC AAGATTGTCG CATTTCTACG CCAACCAAAC 
ATTTTTGAGA TGCTGCAAGA GCGTCAGCCA AGCCTGGCCA GGAACCATGC ACTCAGGGAG 
AAGATCCATT ACATTCGGAC AGAGGGTACA CATGGGCTTG AAAAGCTGTC CTGTGATGCA 
GATTTAGTAA TTCTATTGAG CCTCTTTGAG GAGGATATTA TGTCCTACAT ACCCCTGCAG 
GCTGCCTTCC ACCCTGGCTA TAGCTTCTCT CCTCGCTGTT CACCCTGCTC CTCACCTCAG 
AATTCTCCAG GATTACAGCG AGCTAGTGCA CGAGCGCCCT CTCCATATAG GAGAGACTTT 
GAAGCCAAGC TACGCAATTT CTATCGAAAA CTTGAAGCCA AAGGATATGG ACAAGGTCCA 
GGTAAAATTA AGTTAATTAT ACGGCGTGAC CACTTGTTGG AAGGCACCTT CAATCAGGTG 
ATGGCTTATT CACGCAAGGA GCTTCAGCGG AACAAACTCT ATGTCACATT CGTTGGAGAG 
GAAGGCTTGG ACTACAGCGG TCCCTCCCGA GAGTTCTTCT TCCTTCTGTC TCAGGAGCTC 
TTCAATCCTT ACTATGGCTT GTTTGAGTAT TCAGCCAACG ACACTTACAC TGTACAGATC 
AGCCCCATGT CTGCGTTTGT GGAAAACCAC CTTGAATGGT TCAGGTTCAG CGGTCGTATC 
CTTGGCCTTG CTCTCATTCA TCAGTACCTT CTTGATGCTT TCTTCACAAG GCCGTTCTAT 
AAAGCACTAC TGAGGCTGCC GTGTGATTTA AGTGACCTGG AGTACCTGGA TGAGGAGTTT 
CACCAGAGTT TACAGTGGAT GAAGGATAAC AACATCACTG ATATCCTGGA TCTCACATTC 
ACCGTGAACG AGGAGGTGTT TGGACAGGTG ACAGAAAGGG AGCTGAAATC AGGGGGTTCA 
AATACACAGG TGACAGAAAA GAACAAGAAG GAGTACATTG AGAGAATGGT GAAGTGGCGT 
GTGGAACGAG GAGTGGTTCA GCAAACAGAG GCTCTAGTCC GTGGCTTCTA TGAAGTTGTA 
GACTCCAGGC TTGTCTCCAT TTTTGATGCC AGAGAGCTAG AGTTGGTGAT TGCAGGTACA 
GCAGAAATAG ACCTCAATGA CTGGCGTAAC AACACAGAAT ATCGTGGAGG TTACCATGAT 
GGGCATATTG TCATACGGTG GTTCTGGGCA GCAGTGGAGA GATTTAACAA TGAACAGAGA 
CTACGGTTAC TGCAGTTTGT CACTGGAACA TCTAGTGTAC CATATGAGGG TTTTGCAGCC 
CTTCGAGGGA GCAATGGGTT ACGGCGTTTC TGTATAGAAA AATGGGGGAA AATAACATCT 
CTTCCTAGGG CGCACACATG TTTCAACCGC TTGGATCTCC CGCCCTACCC ATCTTATTCT 
ATGCTGTATG AAAAGCTGTT GACAGCTGTT GAAGAAACTA GCACCTTTGG ACTTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006124158.1
CDS	181..4920
Translation	MASPSRNSQNRRRCKEPLRYSYNPDQFNNMDLRNNAHETVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIYWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDANSYFVESETKICFKYYHGVSGALRATTPSVTVKNSSAPIFKSIVNEDPVQGQGSRRLISFSLSDFQALGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQEKRSKITCNTVNPIWQGEHFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPDDEEVSISADPESAELQESPVNCTAEEILGEPPCTGTVTSQSTSPEQLNGCHVKSVNVDIPGEVKQNSLNEELAGNREVGVVPADATASLESIPGEVLPSLSALELSEQLALLVCTPPPEIDFRENNKVNSVTQGHEDIFTRMEALDTDEVSADIQTVKYLEKIQDEEGASAQEEEDNKLQLRMSGKRKNRPCSLPVSELETVIASACGEPETPRTHYIRIHNLLHSLPSAQMSSDGEEEPGGGENDEESTVKEVSEKDVVSESETLAADPPPESGTDEYFSRGTVPRSTSVEHLSDLNGQLLDGEHPTAGSESESSSRPYGDNECDTSCYSPSCYSTSCYSTSCYSTSCYSPSCYDSNNRFSSHTRFSSVDSAKISESTVFSSQDDEEEENSAFESVPDSVQSPELDREQVDVSAQWPDELIAHGGNSQRATEILESPVAGPSNRREGDCPLLHNSQPVSQLPSLRPDHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGIRRSGSIQQMEQLNRRYQNIQRTIATERPEDDAVVNNRVERLSTGGGSDSETDSSQPNSEIRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTNSTCLKHMILKIRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGISLLTRPGNSLVTAIRNQHHHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHALREKIHYIRTEGTHGLEKLSCDADLVILLSLFEEDIMSYIPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGYGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGSNTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSIFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_006124096.2
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Definition	Pelodiscus sinensis HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006124096.2

ORF Insert Sequence:

ATGGCGTCTC CATCTAGGAA TTCACAGAAC CGGCGCCGGT GTAAAGAACC TCTCAGATAC 
AGCTATAACC CTGACCAGTT TAATAACATG GACCTCAGGA ACAATGCTCA TGAGACAGTA 
ACCATTCCAC GATCCACCAG TGATACTGAC CTGGTCACTT CAGACAGCCG GTCTACTCTC 
ATGGTCAGCA GCTCATATTA CTCCATTGGA CATTCACAAG ACCTAGTTAT CTATTGGGAT 
ATAAAGGAAG AAGTGGATGC AGGGGACTGG ATCGGCATGT ATCTCATTGA TGAAGTCTTG 
TCTGAAAACT TTCTGGACTA CAAAAACCGT GGGGTCAATG GCTCTCATCG TGGTCAGATT 
ATCTGGAAGA TTGATGCCAA CTCTTACTTC GTAGAGTCTG AAACAAAGAT CTGCTTCAAA 
TACTACCATG GAGTGAGTGG AGCACTACGA GCTACGACCC CCAGTGTCAC AGTGAAAAAC 
AGTTCTGCTC CCATTTTTAA AAGCATCGTC AATGAAGATC CAGTCCAGGG TCAAGGAAGT 
CGGAGACTGA TCAGTTTTTC ACTGTCAGAT TTCCAGGCTT TGGGTCTGAA AAAAGGAATG 
TTCTTTAACC CAGACCCATA CCTGAAGATT TCCATTCAGC CTGGAAAGCA CAGCATCTTC 
CCAGCACTTC CTCACCATGG ACAAGAGAAG AGATCCAAGA TCACATGTAA TACTGTGAAC 
CCTATCTGGC AAGGAGAGCA CTTCAGCTTT GTTTCCCTTC CCACGGATGT TCTGGAAATA 
GAAGTTAAGG ACAAGTTTGC AAAGAGCCGG CCAATCATTA AACGCTTCTT GGGGAAACTT 
TCCATGCCAG TGCAACGCCT GCTGGAGAGA CACGCAATAG GGGACAGGGT TGTCAGCTAC 
ACTCTGGGAC GTAGGCTTCC AACAGATCAT GTCAGCGGCC AGCTGCAGTT TCGGTTTGAG 
ATAACTTCTT CTATCCATCC AGATGATGAA GAGGTCTCCA TTAGTGCAGA TCCTGAGTCA 
GCTGAACTGC AGGAAAGCCC TGTGAACTGC ACCGCAGAGG AAATCCTGGG TGAGCCTCCC 
TGCACCGGCA CAGTGACCTC ACAAAGTACA TCTCCAGAGC AGCTAAATGG ATGTCATGTG 
AAAAGTGTCA ATGTTGATAT CCCTGGGGAA GTCAAACAGA ACAGCTTAAA TGAAGAGCTA 
GCAGGAAATA GGGAGGTTGG TGTAGTGCCA GCAGATGCTA CTGCGTCCTT GGAATCCATC 
CCTGGGGAAG TGCTGCCTTC CCTGAGTGCT CTGGAACTCT CTGAGCAGCT GGCTCTGCTG 
GTTTGCACAC CTCCTCCAGA GATTGACTTT AGGGAAAATA ATAAAGTCAA TTCTGTTACT 
CAGGGACACG AGGACATCTT CACCCGCATG GAAGCACTAG ATACCGATGA GGTGAGTGCA 
GACATACAGA CTGTCAAGTA CTTGGAGAAA ATTCAGGATG AGGAAGGCGC TTCAGCCCAG 
GAAGAGGAAG ACAACAAGCT GCAGCTAAGA ATGTCAGGGA AAAGGAAAAA CAGGCCCTGC 
TCCTTGCCTG TGTCTGAGCT GGAGACGGTG ATTGCATCAG CCTGTGGTGA GCCTGAGACC 
CCTCGAACGC ACTACATTCG GATCCACAAT CTGCTTCACA GCTTGCCCTC AGCACAGATG 
AGTAGTGATG GAGAGGAGGA GCCGGGAGGC GGCGAGAATG ACGAAGAGTC AACGGTCAAG 
GAAGTGTCAG AGAAGGATGT GGTTAGCGAG TCTGAGACGC TAGCGGCAGA TCCTCCCCCT 
GAAAGCGGAA CTGATGAGTA TTTCAGCCGG GGGACTGTGC CCCGCAGCAC CTCAGTTGAA 
CACCTCTCAG ACTTAAATGG GCAGCTGTTG GATGGAGAAC ATCCAACGGC TGGAAGTGAG 
AGTGAATCAA GTTCCAGGCC ATACGGGGAC AATGAGTGTG ACACCTCTTG CTACAGCCCA 
TCCTGCTACA GCACCTCCTG CTACAGTACG TCCTGCTACA GCACCTCCTG CTATAGCCCC 
TCCTGTTATG ACAGTAACAA TCGCTTTTCC AGCCATACCC GTTTCTCCTC TGTTGACAGT 
GCAAAGATTT CTGAGAGCAC AGTCTTTTCC TCACAGGATG ATGAGGAAGA GGAAAACAGT 
GCCTTTGAGT CAGTGCCAGA TTCAGTGCAA AGCCCTGAAC TGGACAGGGA ACAAGTGGAT 
GTCTCCGCCC AGTGGCCAGA TGAACTCATA GCTCATGGTG GGAATTCACA AAGAGCAACA 
GAGATCCTAG AATCCCCAGT AGCAGGTCCA AGCAACAGAA GAGAAGGTGA TTGTCCTTTA 
CTCCATAATT CTCAGCCAGT CAGCCAGCTT CCTTCCCTGA GGCCTGACCA TCATCACTAT 
CCAACTATTG ATGAGCCTCT TCCACCAAAC TGGGAGGCTC GGATAGACAG CCATGGAAGG 
GTATTTTATG TGGATCACGT GAATCGAACC ACAACATGGC AGCGTCCTAC AGCAGCAGCC 
ACTCCAGATG GAATACGCAG ATCTGGTTCA ATCCAACAAA TGGAGCAGCT CAATCGGCGG 
TATCAAAATA TTCAGCGGAC TATTGCAACA GAGAGGCCAG AGGATGATGC TGTTGTGAAC 
AACAGAGTTG AGAGGCTCTC CACTGGAGGA GGAAGTGACT CTGAGACAGA CTCTTCCCAG 
CCAAACTCAG AAATTAGGCG AGAAGGGTCC CTTTCTCCAG TGAATTCTCA AAAAATCACC 
TTGCTGCTAC AGTCTCCAGC TGTAAAGTTT ATCACAAACC CTGAATTCTT CACCGTGCTG 
CATGCAAACT ATAGTGCCTA TCGAGTCTTC ACCAACAGCA CCTGCTTGAA GCACATGATT 
CTAAAGATCC GCAGAGATGC TCGGAATTTT GAGCGCTATC AGCACAATAG GGACCTGGTA 
AATTTTATCA ATATGTTTGC TGACACCCGA CTGGAACTTC CACGGGGCTG GGAGATCAAA 
ACAGACCAAC AGGGCAAGTC CTTCTTTGTG GACCACAACA GCCGTGCTAC CACTTTTATC 
GATCCTAGGA TCCCACTTCA AAATGGCCGT CTCCCTAATC ATCTGACTCA CAGGCAGCAT 
CTCCAGAGAC TTCGCAGCTA CAGTGCTGGA GAGGCATCAG AAGTCTCCCG GAATAGAGGC 
ATATCTCTGT TGACCAGACC AGGCAACAGC CTAGTAACAG CTATTCGAAA CCAGCACCAT 
CATGAGTCAT TACCTCTAGC TTACAATGAC AAGATTGTCG CATTTCTACG CCAACCAAAC 
ATTTTTGAGA TGCTGCAAGA GCGTCAGCCA AGCCTGGCCA GGAACCATGC ACTCAGGGAG 
AAGATCCATT ACATTCGGAC AGAGGGTACA CATGGGCTTG AAAAGCTGTC CTGTGATGCA 
GATTTAGTAA TTCTATTGAG CCTCTTTGAG GAGGATATTA TGTCCTACAT ACCCCTGCAG 
GCTGCCTTCC ACCCTGGCTA TAGCTTCTCT CCTCGCTGTT CACCCTGCTC CTCACCTCAG 
AATTCTCCAG GATTACAGCG AGCTAGTGCA CGAGCGCCCT CTCCATATAG GAGAGACTTT 
GAAGCCAAGC TACGCAATTT CTATCGAAAA CTTGAAGCCA AAGGATATGG ACAAGGTCCA 
GGTAAAATTA AGTTAATTAT ACGGCGTGAC CACTTGTTGG AAGGCACCTT CAATCAGGTG 
ATGGCTTATT CACGCAAGGA GCTTCAGCGG AACAAACTCT ATGTCACATT CGTTGGAGAG 
GAAGGCTTGG ACTACAGCGG TCCCTCCCGA GAGTTCTTCT TCCTTCTGTC TCAGGAGCTC 
TTCAATCCTT ACTATGGCTT GTTTGAGTAT TCAGCCAACG ACACTTACAC TGTACAGATC 
AGCCCCATGT CTGCGTTTGT GGAAAACCAC CTTGAATGGT TCAGGTTCAG CGGTCGTATC 
CTTGGCCTTG CTCTCATTCA TCAGTACCTT CTTGATGCTT TCTTCACAAG GCCGTTCTAT 
AAAGCACTAC TGAGGCTGCC GTGTGATTTA AGTGACCTGG AGTACCTGGA TGAGGAGTTT 
CACCAGAGTT TACAGTGGAT GAAGGATAAC AACATCACTG ATATCCTGGA TCTCACATTC 
ACCGTGAACG AGGAGGTGTT TGGACAGGTG ACAGAAAGGG AGCTGAAATC AGGGGGTTCA 
AATACACAGG TGACAGAAAA GAACAAGAAG GAGTACATTG AGAGAATGGT GAAGTGGCGT 
GTGGAACGAG GAGTGGTTCA GCAAACAGAG GCTCTAGTCC GTGGCTTCTA TGAAGTTGTA 
GACTCCAGGC TTGTCTCCAT TTTTGATGCC AGAGAGCTAG AGTTGGTGAT TGCAGGTACA 
GCAGAAATAG ACCTCAATGA CTGGCGTAAC AACACAGAAT ATCGTGGAGG TTACCATGAT 
GGGCATATTG TCATACGGTG GTTCTGGGCA GCAGTGGAGA GATTTAACAA TGAACAGAGA 
CTACGGTTAC TGCAGTTTGT CACTGGAACA TCTAGTGTAC CATATGAGGG TTTTGCAGCC 
CTTCGAGGGA GCAATGGGTT ACGGCGTTTC TGTATAGAAA AATGGGGGAA AATAACATCT 
CTTCCTAGGG CGCACACATG TTTCAACCGC TTGGATCTCC CGCCCTACCC ATCTTATTCT 
ATGCTGTATG AAAAGCTGTT GACAGCTGTT GAAGAAACTA GCACCTTTGG ACTTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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03-6811-6572

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HECW1 cDNA ORF clone, Pelodiscus sinensis(Chinese soft-shelled turtle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム