NEFM cDNA ORF clone, Pelodiscus sinensis(Chinese soft-shelled turtle)

Gene
Clones
gRNAs

Summary

Gene Symbol	NEFM
Entrez Gene ID	102448776
Full Name	neurofilament medium
Gene Type	protein-coding
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014569064.1	XP_014424550.1	LOW QUALITY PROTEIN: neurofilament medium polypeptide
XM_014569064.2	XP_014424550.1	LOW QUALITY PROTEIN: neurofilament medium polypeptide

Pathways from BioSystems
Gene Ontology

The following NEFM gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NEFM cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPe21182	XM_014569064.2 Latest version!	Pelodiscus sinensis neurofilament medium (NEFM), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
View Old Accession Versions >>
OPe21182	XM_014569064.1	Pelodiscus sinensis neurofilament, medium polypeptide (NEFM), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NEFM ( XM_014569064.1 ) cDNA ORF clone, Pelodiscus sinensis(Chinese soft-shelled turtle) -> XP_014424550.1 Pelodiscus sinensis neurofilament, medium polypeptide (NEFM), mRNA.

Price & Availability↑

CloneID	OPe21182
Clone ID Related Accession (Same CDS sequence)	XM_014569064.1 , XM_014569064.2
Accession Version	XM_014569064.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2499bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-11-02
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Product	LOW QUALITY PROTEIN: neurofilament medium polypeptide
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005852623.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pelodiscus sinensis Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGAGCTACA CCCTGGAAAC GCTCGGCAAC CCCTCCTACC GGAGGGTGAC CGAGAGCCGG 
GCCACCTACA CCCGGGCCAG CGCCTCCCCG TCCAGCGGCT TCAGGTCGCA GTCCTGGTCC 
CGGGGCTCTC CGAGCACCGT CTCCTCCTCG TACAAGCGCG CCACGCTGGG CGCGCCGCGG 
CTCTCCTATG GCTCCACCGT GCTGAGCTCC TCGGAGAGCC TGGACCTCAG CCAGTCCTCG 
CTGCTCAACG GGGGCGGCGA CTTCAAGCTG AGCCGCTCCA ACGAGAAGGA GCAGCTGCAG 
GGGCTGAACG ACCGCTTCGC CGGCTACATC GAGAAGGTGC ACGGCCTGGA GCAGCAGAAC 
AAGGCGCTGG AGGCGGAGAT CGCGGCGCTG CGCCAGAAGC AAGCCGGGCG CTCGCAGCTG 
GGCGACGCCT ACGAGCAAGA GCTGCGGGAG CTGCGCGGGA CGCTGGAGCT GGTGAACCAC 
GAGAAGGCGC AGGTCCAGCT GGACTCGGAG CACGTGGACG AGGACATCCA GCGGCTGAAG 
GAGCGCTTCG AGGAGGAGGC TCGCCTGCAC GAGGAGACCG AGGCCACCAT CCGCGCTCTG 
CGCAAGGACA TCGAGGAGGC CTCCATGGTC AAGGTGGAGC TGGACAAGAA GGTGCAGTCG 
CTGCAGGACG AGGTGGCCTT CCTGCGGGGC AACCACGAGG AGGAGGTGGC CGAGCTGCTG 
GCCCAGATCC AGGCGTCCCA TGTCACGGTG GAGAGGAAGG ACTACCTGAA GACCGACCTC 
ACCGCCGCGC TCAAGGAGAT CCGCAGCCAG CTGGAGTGCC ACTCCGACCA GAACATGCAC 
CAGGCCGAGG AATGGTTCCG GTGCCGCTAT GCCAAGCTCA CCGAGGCGGC GGAGCAGAAC 
AAAGAGGCCA TCCGCACGGC CAAGGAGGAG ATCGCCGAGT ACCGCCGGCA GCTGCAGTCC 
AAGAGCGTGG AGCTGGAGTC GGTGCGGGGC ACCAAGGAGT CCCTGGAGCG GCAGCTGAAC 
GACATCGAGG AGCGCCACAA CCACGATCTT ACCACCTACC AGGACACGAT TCAGCAGTTG 
GAAAACGAGC TGCGGGGTAC AAAATGGGAA ATGGCGCGTC ATTTGAGGGA ATACCAGGAT 
CTCCTCAATG TCAAGATGGC TCTGGATATT GAAATTGCTG CATACAGGAA ACTACTAGAG 
GGTGAAGAGA CGAGATTCAG TGCCTTCTCG GGAAGCATTA CTGGTCCCAC ATTTACACAC 
AGGCAACCCA CAGTCACAAT ATCATCTAGT AAAATACAGA AATCCAAAGC GGAGCCGCCA 
AAGCTAAAGG TCCAGCACAA ATTTGTAGAA GAAATCATCG AAGAGACAAA AGTGGAGGAT 
GAGAAGTCAG AAATGGATGA TGCCTTGGCA GCCATTGCAG AAGAACTGGC AAGGAAGGAA 
GAACAGAAGA AGGAAGAAGA AGCCGAAGAG GAAGAAGCCG TGGAAGAGGA GATTGTAGCT 
GAGAAGAAAG CGCCCGTGAA AGCAGCTGCA CCCAAAGCTG AAGAAGAAGA AGAAGAAGAA 
GAAGAGAAAG CAGAAGAAGA GGAGGAAGAG GAGGAAGCTG CAAAATCTGA TGCAGCAGAA 
GAAGGAGGTT CTGAAAAGGA AGAAGCAGAA GAAGCGGGTG AGGATGCTGA GCAGGAAGAG 
GGAGAAGAAG AAGGTGAGGC TGAAGCTGAG GGAGAAAAAG CTGAGGCTGA GCAAGAAAGA 
AAAGGAGAGG AAGAGGGTGA GGAGGCTGCA GTGGAAGAGA AGGTGGAAAA ACTGAAAACA 
CCTCTAGCAA AATCGCCACC TAAATCTCCT GCAGCGGAAG AGGCCAAAGA AGAGCAGAAG 
GAATCAGAAG AAGCTGAAGG AGGAGAAAGT GAAGAAGAGA AAGTGGAGAC GGGAGGTAAA 
GAGGAGGAAA CAAAGGAAGA GAAGAAGGCG GGGTCTCCAG AGAAGACGGG GTCTCCAGAG 
AAGGCAGGAA CTCCAGAGAA GGCGGGATCC CCAGTGAAAG AAGAAAAGGC AGTGGTGGAA 
GAGACCATCT CTGTCACAAA GGTAACAAAA GTCAGCGTAG AGAAAGAGTC CAAAGTGAAG 
GACGAAGGCA GTAAAGGAAA AGAAAGTGAC AAGACCTCCA AGGAATCCAT GAAAGAAGAC 
ATTGCAGTGA ACGGGGAGGT GGATGACAAG GAGGAGGAGA AATCCAAAGA AAAAGAAGTT 
GAAGGGGAAG ACAAGGGTGT GGTCACCAAT GGCCTGGATG TGAGCCCATC CGAAGAGAAG 
AAAGGAAAGG CTGAAGAGAA AGTTGTGGTA ACCAAAAAGG TGGAAAAAAT CACTAGCGAC 
GACGGGGACA GTACGACCAA ATACATCACC AAGTCTGTGA CGGTCACCCA AAAGGTAGAG 
GAACACGAGG AATCTTTTGA AGAGAAACTA GTGTCCACCA AGAAGGTTGA GAAAGTCACT 
TCACATGCTG TTATAAAAGA AGTAAAAGGT AGCGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014424550.1
CDS	27..2525
Translation	MSYTLETLGNPSYRRVTESRATYTRASASPSSGFRSQSWSRGSPSTVSSSYKRATLGAPRLSYGSTVLSSSESLDLSQSSLLNGGGDFKLSRSNEKEQLQGLNDRFAGYIEKVHGLEQQNKALEAEIAALRQKQAGRSQLGDAYEQELRELRGTLELVNHEKAQVQLDSEHVDEDIQRLKERFEEEARLHEETEATIRALRKDIEEASMVKVELDKKVQSLQDEVAFLRGNHEEEVAELLAQIQASHVTVERKDYLKTDLTAALKEIRSQLECHSDQNMHQAEEWFRCRYAKLTEAAEQNKEAIRTAKEEIAEYRRQLQSKSVELESVRGTKESLERQLNDIEERHNHDLTTYQDTIQQLENELRGTKWEMARHLREYQDLLNVKMALDIEIAAYRKLLEGEETRFSAFSGSITGPTFTHRQPTVTISSSKIQKSKAEPPKLKVQHKFVEEIIEETKVEDEKSEMDDALAAIAEELARKEEQKKEEEAEEEEAVEEEIVAEKKAPVKAAAPKAEEEEEEEEEKAEEEEEEEEAAKSDAAEEGGSEKEEAEEAGEDAEQEEGEEEGEAEAEGEKAEAEQERKGEEEGEEAAVEEKVEKLKTPLAKSPPKSPAAEEAKEEQKESEEAEGGESEEEKVETGGKEEETKEEKKAGSPEKTGSPEKAGTPEKAGSPVKEEKAVVEETISVTKVTKVSVEKESKVKDEGSKGKESDKTSKESMKEDIAVNGEVDDKEEEKSKEKEVEGEDKGVVTNGLDVSPSEEKKGKAEEKVVVTKKVEKITSDDGDSTTKYITKSVTVTQKVEEHEESFEEKLVSTKKVEKVTSHAVIKEVKGSD

Target ORF information:

RefSeq Version	XM_014569064.1
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Definition	Pelodiscus sinensis neurofilament, medium polypeptide (NEFM), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014569064.1

ORF Insert Sequence:

ATGAGCTACA CCCTGGAAAC GCTCGGCAAC CCCTCCTACC GGAGGGTGAC CGAGAGCCGG 
GCCACCTACA CCCGGGCCAG CGCCTCCCCG TCCAGCGGCT TCAGGTCGCA GTCCTGGTCC 
CGGGGCTCTC CGAGCACCGT CTCCTCCTCG TACAAGCGCG CCACGCTGGG CGCGCCGCGG 
CTCTCCTATG GCTCCACCGT GCTGAGCTCC TCGGAGAGCC TGGACCTCAG CCAGTCCTCG 
CTGCTCAACG GGGGCGGCGA CTTCAAGCTG AGCCGCTCCA ACGAGAAGGA GCAGCTGCAG 
GGGCTGAACG ACCGCTTCGC CGGCTACATC GAGAAGGTGC ACGGCCTGGA GCAGCAGAAC 
AAGGCGCTGG AGGCGGAGAT CGCGGCGCTG CGCCAGAAGC AAGCCGGGCG CTCGCAGCTG 
GGCGACGCCT ACGAGCAAGA GCTGCGGGAG CTGCGCGGGA CGCTGGAGCT GGTGAACCAC 
GAGAAGGCGC AGGTCCAGCT GGACTCGGAG CACGTGGACG AGGACATCCA GCGGCTGAAG 
GAGCGCTTCG AGGAGGAGGC TCGCCTGCAC GAGGAGACCG AGGCCACCAT CCGCGCTCTG 
CGCAAGGACA TCGAGGAGGC CTCCATGGTC AAGGTGGAGC TGGACAAGAA GGTGCAGTCG 
CTGCAGGACG AGGTGGCCTT CCTGCGGGGC AACCACGAGG AGGAGGTGGC CGAGCTGCTG 
GCCCAGATCC AGGCGTCCCA TGTCACGGTG GAGAGGAAGG ACTACCTGAA GACCGACCTC 
ACCGCCGCGC TCAAGGAGAT CCGCAGCCAG CTGGAGTGCC ACTCCGACCA GAACATGCAC 
CAGGCCGAGG AATGGTTCCG GTGCCGCTAT GCCAAGCTCA CCGAGGCGGC GGAGCAGAAC 
AAAGAGGCCA TCCGCACGGC CAAGGAGGAG ATCGCCGAGT ACCGCCGGCA GCTGCAGTCC 
AAGAGCGTGG AGCTGGAGTC GGTGCGGGGC ACCAAGGAGT CCCTGGAGCG GCAGCTGAAC 
GACATCGAGG AGCGCCACAA CCACGATCTT ACCACCTACC AGGACACGAT TCAGCAGTTG 
GAAAACGAGC TGCGGGGTAC AAAATGGGAA ATGGCGCGTC ATTTGAGGGA ATACCAGGAT 
CTCCTCAATG TCAAGATGGC TCTGGATATT GAAATTGCTG CATACAGGAA ACTACTAGAG 
GGTGAAGAGA CGAGATTCAG TGCCTTCTCG GGAAGCATTA CTGGTCCCAC ATTTACACAC 
AGGCAACCCA CAGTCACAAT ATCATCTAGT AAAATACAGA AATCCAAAGC GGAGCCGCCA 
AAGCTAAAGG TCCAGCACAA ATTTGTAGAA GAAATCATCG AAGAGACAAA AGTGGAGGAT 
GAGAAGTCAG AAATGGATGA TGCCTTGGCA GCCATTGCAG AAGAACTGGC AAGGAAGGAA 
GAACAGAAGA AGGAAGAAGA AGCCGAAGAG GAAGAAGCCG TGGAAGAGGA GATTGTAGCT 
GAGAAGAAAG CGCCCGTGAA AGCAGCTGCA CCCAAAGCTG AAGAAGAAGA AGAAGAAGAA 
GAAGAGAAAG CAGAAGAAGA GGAGGAAGAG GAGGAAGCTG CAAAATCTGA TGCAGCAGAA 
GAAGGAGGTT CTGAAAAGGA AGAAGCAGAA GAAGCGGGTG AGGATGCTGA GCAGGAAGAG 
GGAGAAGAAG AAGGTGAGGC TGAAGCTGAG GGAGAAAAAG CTGAGGCTGA GCAAGAAAGA 
AAAGGAGAGG AAGAGGGTGA GGAGGCTGCA GTGGAAGAGA AGGTGGAAAA ACTGAAAACA 
CCTCTAGCAA AATCGCCACC TAAATCTCCT GCAGCGGAAG AGGCCAAAGA AGAGCAGAAG 
GAATCAGAAG AAGCTGAAGG AGGAGAAAGT GAAGAAGAGA AAGTGGAGAC GGGAGGTAAA 
GAGGAGGAAA CAAAGGAAGA GAAGAAGGCG GGGTCTCCAG AGAAGACGGG GTCTCCAGAG 
AAGGCAGGAA CTCCAGAGAA GGCGGGATCC CCAGTGAAAG AAGAAAAGGC AGTGGTGGAA 
GAGACCATCT CTGTCACAAA GGTAACAAAA GTCAGCGTAG AGAAAGAGTC CAAAGTGAAG 
GACGAAGGCA GTAAAGGAAA AGAAAGTGAC AAGACCTCCA AGGAATCCAT GAAAGAAGAC 
ATTGCAGTGA ACGGGGAGGT GGATGACAAG GAGGAGGAGA AATCCAAAGA AAAAGAAGTT 
GAAGGGGAAG ACAAGGGTGT GGTCACCAAT GGCCTGGATG TGAGCCCATC CGAAGAGAAG 
AAAGGAAAGG CTGAAGAGAA AGTTGTGGTA ACCAAAAAGG TGGAAAAAAT CACTAGCGAC 
GACGGGGACA GTACGACCAA ATACATCACC AAGTCTGTGA CGGTCACCCA AAAGGTAGAG 
GAACACGAGG AATCTTTTGA AGAGAAACTA GTGTCCACCA AGAAGGTTGA GAAAGTCACT 
TCACATGCTG TTATAAAAGA AGTAAAAGGT AGCGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEFM ( XM_014569064.2 ) cDNA ORF clone, Pelodiscus sinensis(Chinese soft-shelled turtle) -> XP_014424550.1 Pelodiscus sinensis neurofilament medium (NEFM), mRNA.

Price & Availability↑

CloneID	OPe21182
Clone ID Related Accession (Same CDS sequence)	XM_014569064.1 , XM_014569064.2
Accession Version	XM_014569064.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2499bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-03
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Product	LOW QUALITY PROTEIN: neurofilament medium polypeptide
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005852623.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 4, 2018 this sequence version replaced XM_014569064.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pelodiscus sinensis Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGAGCTACA CCCTGGAAAC GCTCGGCAAC CCCTCCTACC GGAGGGTGAC CGAGAGCCGG 
GCCACCTACA CCCGGGCCAG CGCCTCCCCG TCCAGCGGCT TCAGGTCGCA GTCCTGGTCC 
CGGGGCTCTC CGAGCACCGT CTCCTCCTCG TACAAGCGCG CCACGCTGGG CGCGCCGCGG 
CTCTCCTATG GCTCCACCGT GCTGAGCTCC TCGGAGAGCC TGGACCTCAG CCAGTCCTCG 
CTGCTCAACG GGGGCGGCGA CTTCAAGCTG AGCCGCTCCA ACGAGAAGGA GCAGCTGCAG 
GGGCTGAACG ACCGCTTCGC CGGCTACATC GAGAAGGTGC ACGGCCTGGA GCAGCAGAAC 
AAGGCGCTGG AGGCGGAGAT CGCGGCGCTG CGCCAGAAGC AAGCCGGGCG CTCGCAGCTG 
GGCGACGCCT ACGAGCAAGA GCTGCGGGAG CTGCGCGGGA CGCTGGAGCT GGTGAACCAC 
GAGAAGGCGC AGGTCCAGCT GGACTCGGAG CACGTGGACG AGGACATCCA GCGGCTGAAG 
GAGCGCTTCG AGGAGGAGGC TCGCCTGCAC GAGGAGACCG AGGCCACCAT CCGCGCTCTG 
CGCAAGGACA TCGAGGAGGC CTCCATGGTC AAGGTGGAGC TGGACAAGAA GGTGCAGTCG 
CTGCAGGACG AGGTGGCCTT CCTGCGGGGC AACCACGAGG AGGAGGTGGC CGAGCTGCTG 
GCCCAGATCC AGGCGTCCCA TGTCACGGTG GAGAGGAAGG ACTACCTGAA GACCGACCTC 
ACCGCCGCGC TCAAGGAGAT CCGCAGCCAG CTGGAGTGCC ACTCCGACCA GAACATGCAC 
CAGGCCGAGG AATGGTTCCG GTGCCGCTAT GCCAAGCTCA CCGAGGCGGC GGAGCAGAAC 
AAAGAGGCCA TCCGCACGGC CAAGGAGGAG ATCGCCGAGT ACCGCCGGCA GCTGCAGTCC 
AAGAGCGTGG AGCTGGAGTC GGTGCGGGGC ACCAAGGAGT CCCTGGAGCG GCAGCTGAAC 
GACATCGAGG AGCGCCACAA CCACGATCTT ACCACCTACC AGGACACGAT TCAGCAGTTG 
GAAAACGAGC TGCGGGGTAC AAAATGGGAA ATGGCGCGTC ATTTGAGGGA ATACCAGGAT 
CTCCTCAATG TCAAGATGGC TCTGGATATT GAAATTGCTG CATACAGGAA ACTACTAGAG 
GGTGAAGAGA CGAGATTCAG TGCCTTCTCG GGAAGCATTA CTGGTCCCAC ATTTACACAC 
AGGCAACCCA CAGTCACAAT ATCATCTAGT AAAATACAGA AATCCAAAGC GGAGCCGCCA 
AAGCTAAAGG TCCAGCACAA ATTTGTAGAA GAAATCATCG AAGAGACAAA AGTGGAGGAT 
GAGAAGTCAG AAATGGATGA TGCCTTGGCA GCCATTGCAG AAGAACTGGC AAGGAAGGAA 
GAACAGAAGA AGGAAGAAGA AGCCGAAGAG GAAGAAGCCG TGGAAGAGGA GATTGTAGCT 
GAGAAGAAAG CGCCCGTGAA AGCAGCTGCA CCCAAAGCTG AAGAAGAAGA AGAAGAAGAA 
GAAGAGAAAG CAGAAGAAGA GGAGGAAGAG GAGGAAGCTG CAAAATCTGA TGCAGCAGAA 
GAAGGAGGTT CTGAAAAGGA AGAAGCAGAA GAAGCGGGTG AGGATGCTGA GCAGGAAGAG 
GGAGAAGAAG AAGGTGAGGC TGAAGCTGAG GGAGAAAAAG CTGAGGCTGA GCAAGAAAGA 
AAAGGAGAGG AAGAGGGTGA GGAGGCTGCA GTGGAAGAGA AGGTGGAAAA ACTGAAAACA 
CCTCTAGCAA AATCGCCACC TAAATCTCCT GCAGCGGAAG AGGCCAAAGA AGAGCAGAAG 
GAATCAGAAG AAGCTGAAGG AGGAGAAAGT GAAGAAGAGA AAGTGGAGAC GGGAGGTAAA 
GAGGAGGAAA CAAAGGAAGA GAAGAAGGCG GGGTCTCCAG AGAAGACGGG GTCTCCAGAG 
AAGGCAGGAA CTCCAGAGAA GGCGGGATCC CCAGTGAAAG AAGAAAAGGC AGTGGTGGAA 
GAGACCATCT CTGTCACAAA GGTAACAAAA GTCAGCGTAG AGAAAGAGTC CAAAGTGAAG 
GACGAAGGCA GTAAAGGAAA AGAAAGTGAC AAGACCTCCA AGGAATCCAT GAAAGAAGAC 
ATTGCAGTGA ACGGGGAGGT GGATGACAAG GAGGAGGAGA AATCCAAAGA AAAAGAAGTT 
GAAGGGGAAG ACAAGGGTGT GGTCACCAAT GGCCTGGATG TGAGCCCATC CGAAGAGAAG 
AAAGGAAAGG CTGAAGAGAA AGTTGTGGTA ACCAAAAAGG TGGAAAAAAT CACTAGCGAC 
GACGGGGACA GTACGACCAA ATACATCACC AAGTCTGTGA CGGTCACCCA AAAGGTAGAG 
GAACACGAGG AATCTTTTGA AGAGAAACTA GTGTCCACCA AGAAGGTTGA GAAAGTCACT 
TCACATGCTG TTATAAAAGA AGTAAAAGGT AGCGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014424550.1
CDS	27..2525
Translation	MSYTLETLGNPSYRRVTESRATYTRASASPSSGFRSQSWSRGSPSTVSSSYKRATLGAPRLSYGSTVLSSSESLDLSQSSLLNGGGDFKLSRSNEKEQLQGLNDRFAGYIEKVHGLEQQNKALEAEIAALRQKQAGRSQLGDAYEQELRELRGTLELVNHEKAQVQLDSEHVDEDIQRLKERFEEEARLHEETEATIRALRKDIEEASMVKVELDKKVQSLQDEVAFLRGNHEEEVAELLAQIQASHVTVERKDYLKTDLTAALKEIRSQLECHSDQNMHQAEEWFRCRYAKLTEAAEQNKEAIRTAKEEIAEYRRQLQSKSVELESVRGTKESLERQLNDIEERHNHDLTTYQDTIQQLENELRGTKWEMARHLREYQDLLNVKMALDIEIAAYRKLLEGEETRFSAFSGSITGPTFTHRQPTVTISSSKIQKSKAEPPKLKVQHKFVEEIIEETKVEDEKSEMDDALAAIAEELARKEEQKKEEEAEEEEAVEEEIVAEKKAPVKAAAPKAEEEEEEEEEKAEEEEEEEEAAKSDAAEEGGSEKEEAEEAGEDAEQEEGEEEGEAEAEGEKAEAEQERKGEEEGEEAAVEEKVEKLKTPLAKSPPKSPAAEEAKEEQKESEEAEGGESEEEKVETGGKEEETKEEKKAGSPEKTGSPEKAGTPEKAGSPVKEEKAVVEETISVTKVTKVSVEKESKVKDEGSKGKESDKTSKESMKEDIAVNGEVDDKEEEKSKEKEVEGEDKGVVTNGLDVSPSEEKKGKAEEKVVVTKKVEKITSDDGDSTTKYITKSVTVTQKVEEHEESFEEKLVSTKKVEKVTSHAVIKEVKGSD

Target ORF information:

RefSeq Version	XM_014569064.2
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Definition	Pelodiscus sinensis neurofilament medium (NEFM), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014569064.2

ORF Insert Sequence:

ATGAGCTACA CCCTGGAAAC GCTCGGCAAC CCCTCCTACC GGAGGGTGAC CGAGAGCCGG 
GCCACCTACA CCCGGGCCAG CGCCTCCCCG TCCAGCGGCT TCAGGTCGCA GTCCTGGTCC 
CGGGGCTCTC CGAGCACCGT CTCCTCCTCG TACAAGCGCG CCACGCTGGG CGCGCCGCGG 
CTCTCCTATG GCTCCACCGT GCTGAGCTCC TCGGAGAGCC TGGACCTCAG CCAGTCCTCG 
CTGCTCAACG GGGGCGGCGA CTTCAAGCTG AGCCGCTCCA ACGAGAAGGA GCAGCTGCAG 
GGGCTGAACG ACCGCTTCGC CGGCTACATC GAGAAGGTGC ACGGCCTGGA GCAGCAGAAC 
AAGGCGCTGG AGGCGGAGAT CGCGGCGCTG CGCCAGAAGC AAGCCGGGCG CTCGCAGCTG 
GGCGACGCCT ACGAGCAAGA GCTGCGGGAG CTGCGCGGGA CGCTGGAGCT GGTGAACCAC 
GAGAAGGCGC AGGTCCAGCT GGACTCGGAG CACGTGGACG AGGACATCCA GCGGCTGAAG 
GAGCGCTTCG AGGAGGAGGC TCGCCTGCAC GAGGAGACCG AGGCCACCAT CCGCGCTCTG 
CGCAAGGACA TCGAGGAGGC CTCCATGGTC AAGGTGGAGC TGGACAAGAA GGTGCAGTCG 
CTGCAGGACG AGGTGGCCTT CCTGCGGGGC AACCACGAGG AGGAGGTGGC CGAGCTGCTG 
GCCCAGATCC AGGCGTCCCA TGTCACGGTG GAGAGGAAGG ACTACCTGAA GACCGACCTC 
ACCGCCGCGC TCAAGGAGAT CCGCAGCCAG CTGGAGTGCC ACTCCGACCA GAACATGCAC 
CAGGCCGAGG AATGGTTCCG GTGCCGCTAT GCCAAGCTCA CCGAGGCGGC GGAGCAGAAC 
AAAGAGGCCA TCCGCACGGC CAAGGAGGAG ATCGCCGAGT ACCGCCGGCA GCTGCAGTCC 
AAGAGCGTGG AGCTGGAGTC GGTGCGGGGC ACCAAGGAGT CCCTGGAGCG GCAGCTGAAC 
GACATCGAGG AGCGCCACAA CCACGATCTT ACCACCTACC AGGACACGAT TCAGCAGTTG 
GAAAACGAGC TGCGGGGTAC AAAATGGGAA ATGGCGCGTC ATTTGAGGGA ATACCAGGAT 
CTCCTCAATG TCAAGATGGC TCTGGATATT GAAATTGCTG CATACAGGAA ACTACTAGAG 
GGTGAAGAGA CGAGATTCAG TGCCTTCTCG GGAAGCATTA CTGGTCCCAC ATTTACACAC 
AGGCAACCCA CAGTCACAAT ATCATCTAGT AAAATACAGA AATCCAAAGC GGAGCCGCCA 
AAGCTAAAGG TCCAGCACAA ATTTGTAGAA GAAATCATCG AAGAGACAAA AGTGGAGGAT 
GAGAAGTCAG AAATGGATGA TGCCTTGGCA GCCATTGCAG AAGAACTGGC AAGGAAGGAA 
GAACAGAAGA AGGAAGAAGA AGCCGAAGAG GAAGAAGCCG TGGAAGAGGA GATTGTAGCT 
GAGAAGAAAG CGCCCGTGAA AGCAGCTGCA CCCAAAGCTG AAGAAGAAGA AGAAGAAGAA 
GAAGAGAAAG CAGAAGAAGA GGAGGAAGAG GAGGAAGCTG CAAAATCTGA TGCAGCAGAA 
GAAGGAGGTT CTGAAAAGGA AGAAGCAGAA GAAGCGGGTG AGGATGCTGA GCAGGAAGAG 
GGAGAAGAAG AAGGTGAGGC TGAAGCTGAG GGAGAAAAAG CTGAGGCTGA GCAAGAAAGA 
AAAGGAGAGG AAGAGGGTGA GGAGGCTGCA GTGGAAGAGA AGGTGGAAAA ACTGAAAACA 
CCTCTAGCAA AATCGCCACC TAAATCTCCT GCAGCGGAAG AGGCCAAAGA AGAGCAGAAG 
GAATCAGAAG AAGCTGAAGG AGGAGAAAGT GAAGAAGAGA AAGTGGAGAC GGGAGGTAAA 
GAGGAGGAAA CAAAGGAAGA GAAGAAGGCG GGGTCTCCAG AGAAGACGGG GTCTCCAGAG 
AAGGCAGGAA CTCCAGAGAA GGCGGGATCC CCAGTGAAAG AAGAAAAGGC AGTGGTGGAA 
GAGACCATCT CTGTCACAAA GGTAACAAAA GTCAGCGTAG AGAAAGAGTC CAAAGTGAAG 
GACGAAGGCA GTAAAGGAAA AGAAAGTGAC AAGACCTCCA AGGAATCCAT GAAAGAAGAC 
ATTGCAGTGA ACGGGGAGGT GGATGACAAG GAGGAGGAGA AATCCAAAGA AAAAGAAGTT 
GAAGGGGAAG ACAAGGGTGT GGTCACCAAT GGCCTGGATG TGAGCCCATC CGAAGAGAAG 
AAAGGAAAGG CTGAAGAGAA AGTTGTGGTA ACCAAAAAGG TGGAAAAAAT CACTAGCGAC 
GACGGGGACA GTACGACCAA ATACATCACC AAGTCTGTGA CGGTCACCCA AAAGGTAGAG 
GAACACGAGG AATCTTTTGA AGAGAAACTA GTGTCCACCA AGAAGGTTGA GAAAGTCACT 
TCACATGCTG TTATAAAAGA AGTAAAAGGT AGCGATTAA