CRIM1 cDNA ORF clone, Pelodiscus sinensis(Chinese soft-shelled turtle)

Gene
Clones
gRNAs

Summary

Gene Symbol	CRIM1
Entrez Gene ID	102446780
Full Name	cysteine rich transmembrane BMP regulator 1
Gene Type	protein-coding
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006131287.3	XP_006131349.1	cysteine-rich motor neuron 1 protein
XM_006131287.2	XP_006131349.1	cysteine-rich motor neuron 1 protein

Pathways from BioSystems
Gene Ontology

The following CRIM1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CRIM1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPe36732	XM_006131287.3 Latest version!	Pelodiscus sinensis cysteine rich transmembrane BMP regulator 1 (CRIM1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
View Old Accession Versions >>
OPe36732	XM_006131287.2	Pelodiscus sinensis cysteine rich transmembrane BMP regulator 1 (chordin-like) (CRIM1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CRIM1 ( XM_006131287.3 ) cDNA ORF clone, Pelodiscus sinensis(Chinese soft-shelled turtle) -> XP_006131349.1 Pelodiscus sinensis cysteine rich transmembrane BMP regulator 1 (CRIM1), mRNA.

Price & Availability↑

CloneID	OPe36732
Clone ID Related Accession (Same CDS sequence)	XM_006131287.3 , XM_006131287.2
Accession Version	XM_006131287.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3117bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-03
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Product	cysteine-rich motor neuron 1 protein
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005857385.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 4, 2018 this sequence version replaced XM_006131287.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pelodiscus sinensis Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACCTGG CAGCGGTCTT GGCAGGGCGG AGGCGCCCGG GCGGCGGCTG GCACCTGGCG 
GCGGGGTGGC TGCTCTTGGT GCTGCTGGGC GCGTCCGGGA CGCGGGCGCT CGTCTGCCTC 
CCCTGCGACG AGTCCAAGTG CGAGGAGCCC AAGAGCTGCC CCGGCAGCAT CGTGCTGGGC 
ATCTGCGGCT GCTGCTTCAT GTGCGCCCGG CAGCGCAACG AGAGCTGCGG GGGCGTCTAC 
GGGCTGCACG GAGCCTGCGA CCGCGGGCTG CGCTGTGTCA TCCGCCCCCC GCTCAACGGG 
GACTCCATCA CCGAGTACGA AGTGGGCGTC TGCGAAGATG AAAATTGGGA TGATGATCAG 
CTACTTGGAT TTGAACCTTG CAATGAAAAC CTTGTAACAG GTTGCAACAT AATCAATGGG 
AAATGTGAAT GTGACACCAT TCGCACCTGT AGTAATCCAT TTGAGTTTCC AAGCCAGGAT 
ACTTGCCTGT CAGCTTTAAA AAGGATTGAA GAAGAGAAAC CTGACTGTTC CAAGGCCCGC 
TGTGAGGTCC AGTTTTCTCC TCGCTGTCCA GAAGATTCCA TCTTGATTGA AGGTTATGCT 
CCTCCAGGGG AATGCTGTCC ACTTCCAAGC CGTTGTGTTT GCAACCCTGC AGGTTGCCTG 
AGGAAAGTTT GCCAGCCAGG CTATTTGAAT ATATTGGTTT CTAAAGCATC AGGGAAACCA 
GGGGAGTGCT GTGATCTCTA CGAATGTAAA CCAGTTTTCA GTGTGGATTG CAGCACCGTG 
GAATGCCCCC CGGTTCAACA AGTTGTTTGT CCACTGGATA GCTATGAAAC CCAGGTCAGG 
TTGACAGCTG ATGGCTGCTG TACTCTTCCC ACGAGATGCG AGTGTCTCTC TGGTTTATGT 
GGTTTCCCCG TGTGCGAGGC AGGCTCCATT CCCCAGATAG TCTCACGTGG AGATGGAACA 
CCCGGCAAGT GCTGTGATGT CTTTGAATGT GTCAATGAAG TGAAGCCAGC TTGCATATTT 
AACAGTGTGG AGTATTATGA TGGTGACATG TTTCGAATGG ATGCCTGCCG TTTCTGTCGA 
TGTCAGGGTG GCGTCTCGAT TTGTTTCTCG GCCCAGTGTG GCGAGTTGCA CTGTGACAGG 
TACTATGTTC CAGAAGGAGA GTGCTGTCCA GTTTGTGAAG ATCCAGTATA TCCAGTTAAT 
AACCCTGCTG GCTGCTATGC CAATGGTCAG ATCCAAGCAC ATGGAGATCG CTGGAGAGAA 
GATGACTGCA CTTTCTGTCA GTGTATCAAT GGAGATCCCC ACTGTGTTGC AACAGCCTGT 
GGACAGAGTT GCCTGAATCC TGTTAAAGTA CCCGGAGAAT GCTGCCCAGT GTGTGAAGAA 
CCAACATACA TCACAATAGG TCCACCCACG TGTGAGATTT TGGTGAACTG CACATTGACT 
GAGAGAGACT GTATGTACAG TTTCAGACTG GACCAAAACG GTTGCCGCAC TTGTCAGTGC 
AAAACTAGAG AGGAGCTGTG TACTGGCCTC ATATCAGGGT GTTCCTTGGA CTGTTCCTTC 
GGTTTCCAGA CTGATGTCCA CAACTGTGAG ATCTGTCAGT GCCGTCCAAG GCCTAAGAAA 
TGCAAACCTA TAGTATGTGA CAAGTACTGT CCATTTGGAT ACTTGAAGAA CAAGCATGGC 
TGTGATACCT GTCGCTGTAA GAAATGCCCA GAAGTTCCTT GTGGTAAGAT CTGTCCAATG 
GGCTTCCAGC AGAATAATCA TGGTTGTCTT CTCTGCAAGT GCAGAGAGAT ACCAGATTCT 
GTTATGCCAC CAGTTAAAAC AGGCTCTTGC CTGTCAATGG ATGGTCATCG TCATGAGAAA 
GAGGAGAGTT GGCATGATGG TTGCCGAGAA TGTTATTGTC TCAATGGACG GGAGATGTGT 
GCGTTGATCA CCTGCCCTGT GCCAAACTGT GGCAATCCTA CCATACATCC AGGACAATGT 
TGTCCATCAT GTCCAGATGA ATCTATTGTG CAGAAGCCAG AGCTTAGCAG TCCATCTATC 
TGCCATGCTC CAGGAGGAGA ATACTTTGTA GAAGGAGAGA CATGGAATAT TGACACCTGC 
ACACAGTGTA CATGCCATAG TGGACGCGTT CTGTGTGAGA CCGAAGTCTG CCCACCTCTT 
CTTTGTCAAA ACCCCATCCG NNNNNNNNNN NTCTGTCTAT TCATGTTTCC AGATGAACCT 
CTTCAGCCCT CCTCATCAAG CAACGAAAGC ATGCCCAGTT ACTGTAAAAA CGACGAAGGA 
GATATTTTCC TGACAGCTGA GTCCTGGAAA CCCAATGTCT GCACTAGTTG TATTTGCATG 
GATGGTGTGA TTAGCTGCTA CTCTGAATCT TGCCCTCCAG TATCCTGTGA GAGACCTGTT 
TTGAGAAAAG GACAGTGTTG CCCTTATTGT ATAGAAGATA CAATACCAAA GAGGGTGGTA 
TGTCACTTCA GTGGAAAAAC ATATGCAGAT GAGGAACGTT GGGACATCGA CAGCTGCACA 
CACTGCTACT GCCTGCAGGG GCAGACCCTT TGCTCCACTG TCAGTTGTCC TCCTCTCCCC 
TGTGTAGAAC CCATCAACGT TGAGGGAAGT TGCTGTCCCA TGTGTCCAGA GATGTATGTA 
CCTGAGCCCA CCAACGTCCC CATTGAGAAA ACAAATCATC GAGGAGATAC TGAACTAGAA 
GCACCAATTT GGCCTACACC TAGTGAAAAT GACATTGTTC ATCTCCATAG AGACATGAGT 
CATCTTCAAG CAGATTTCAG AGAAGGCATT GAATTGCACC CCAGTGAAGA CACATCTCTC 
AACTCTGTTG CCTGGGTGAC AGTTCCCATC ATGATAGCCC TAGTTCTTAT AATAGCCCTT 
CTGCTTGTCA ATCAGAAGAA GCAGTGGATA CCAGTGTCCT GTTACAGAGC CCCAACAAAG 
TCTTCTTGCC TAAACAATCA GCTGGTATAT GTGGACTGCA AGAAAGGTAC CATGGTTCAG 
GTGGATAGTT CTCAGAGGAT GCTAAGAATT GCCGACCCAG ATTCAAGATA CAGTGGATTC 
TACAGCATGC AAAAACAGAA CAACCTACAG GCAGATAATT TCTATCAAAC AGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006131349.1
CDS	404..3520
Translation	MYLAAVLAGRRRPGGGWHLAAGWLLLVLLGASGTRALVCLPCDESKCEEPKSCPGSIVLGICGCCFMCARQRNESCGGVYGLHGACDRGLRCVIRPPLNGDSITEYEVGVCEDENWDDDQLLGFEPCNENLVTGCNIINGKCECDTIRTCSNPFEFPSQDTCLSALKRIEEEKPDCSKARCEVQFSPRCPEDSILIEGYAPPGECCPLPSRCVCNPAGCLRKVCQPGYLNILVSKASGKPGECCDLYECKPVFSVDCSTVECPPVQQVVCPLDSYETQVRLTADGCCTLPTRCECLSGLCGFPVCEAGSIPQIVSRGDGTPGKCCDVFECVNEVKPACIFNSVEYYDGDMFRMDACRFCRCQGGVSICFSAQCGELHCDRYYVPEGECCPVCEDPVYPVNNPAGCYANGQIQAHGDRWREDDCTFCQCINGDPHCVATACGQSCLNPVKVPGECCPVCEEPTYITIGPPTCEILVNCTLTERDCMYSFRLDQNGCRTCQCKTREELCTGLISGCSLDCSFGFQTDVHNCEICQCRPRPKKCKPIVCDKYCPFGYLKNKHGCDTCRCKKCPEVPCGKICPMGFQQNNHGCLLCKCREIPDSVMPPVKTGSCLSMDGHRHEKEESWHDGCRECYCLNGREMCALITCPVPNCGNPTIHPGQCCPSCPDESIVQKPELSSPSICHAPGGEYFVEGETWNIDTCTQCTCHSGRVLCETEVCPPLLCQNPInullnullnullnullnullCLFMFPDEPLQPSSSSNESMPSYCKNDEGDIFLTAESWKPNVCTSCICMDGVISCYSESCPPVSCERPVLRKGQCCPYCIEDTIPKRVVCHFSGKTYADEERWDIDSCTHCYCLQGQTLCSTVSCPPLPCVEPINVEGSCCPMCPEMYVPEPTNVPIEKTNHRGDTELEAPIWPTPSENDIVHLHRDMSHLQADFREGIELHPSEDTSLNSVAWVTVPIMIALVLIIALLLVNQKKQWIPVSCYRAPTKSSCLNNQLVYVDCKKGTMVQVDSSQRMLRIADPDSRYSGFYSMQKQNNLQADNFYQTV

Target ORF information:

RefSeq Version	XM_006131287.3
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Definition	Pelodiscus sinensis cysteine rich transmembrane BMP regulator 1 (CRIM1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006131287.3

ORF Insert Sequence:

ATGTACCTGG CAGCGGTCTT GGCAGGGCGG AGGCGCCCGG GCGGCGGCTG GCACCTGGCG 
GCGGGGTGGC TGCTCTTGGT GCTGCTGGGC GCGTCCGGGA CGCGGGCGCT CGTCTGCCTC 
CCCTGCGACG AGTCCAAGTG CGAGGAGCCC AAGAGCTGCC CCGGCAGCAT CGTGCTGGGC 
ATCTGCGGCT GCTGCTTCAT GTGCGCCCGG CAGCGCAACG AGAGCTGCGG GGGCGTCTAC 
GGGCTGCACG GAGCCTGCGA CCGCGGGCTG CGCTGTGTCA TCCGCCCCCC GCTCAACGGG 
GACTCCATCA CCGAGTACGA AGTGGGCGTC TGCGAAGATG AAAATTGGGA TGATGATCAG 
CTACTTGGAT TTGAACCTTG CAATGAAAAC CTTGTAACAG GTTGCAACAT AATCAATGGG 
AAATGTGAAT GTGACACCAT TCGCACCTGT AGTAATCCAT TTGAGTTTCC AAGCCAGGAT 
ACTTGCCTGT CAGCTTTAAA AAGGATTGAA GAAGAGAAAC CTGACTGTTC CAAGGCCCGC 
TGTGAGGTCC AGTTTTCTCC TCGCTGTCCA GAAGATTCCA TCTTGATTGA AGGTTATGCT 
CCTCCAGGGG AATGCTGTCC ACTTCCAAGC CGTTGTGTTT GCAACCCTGC AGGTTGCCTG 
AGGAAAGTTT GCCAGCCAGG CTATTTGAAT ATATTGGTTT CTAAAGCATC AGGGAAACCA 
GGGGAGTGCT GTGATCTCTA CGAATGTAAA CCAGTTTTCA GTGTGGATTG CAGCACCGTG 
GAATGCCCCC CGGTTCAACA AGTTGTTTGT CCACTGGATA GCTATGAAAC CCAGGTCAGG 
TTGACAGCTG ATGGCTGCTG TACTCTTCCC ACGAGATGCG AGTGTCTCTC TGGTTTATGT 
GGTTTCCCCG TGTGCGAGGC AGGCTCCATT CCCCAGATAG TCTCACGTGG AGATGGAACA 
CCCGGCAAGT GCTGTGATGT CTTTGAATGT GTCAATGAAG TGAAGCCAGC TTGCATATTT 
AACAGTGTGG AGTATTATGA TGGTGACATG TTTCGAATGG ATGCCTGCCG TTTCTGTCGA 
TGTCAGGGTG GCGTCTCGAT TTGTTTCTCG GCCCAGTGTG GCGAGTTGCA CTGTGACAGG 
TACTATGTTC CAGAAGGAGA GTGCTGTCCA GTTTGTGAAG ATCCAGTATA TCCAGTTAAT 
AACCCTGCTG GCTGCTATGC CAATGGTCAG ATCCAAGCAC ATGGAGATCG CTGGAGAGAA 
GATGACTGCA CTTTCTGTCA GTGTATCAAT GGAGATCCCC ACTGTGTTGC AACAGCCTGT 
GGACAGAGTT GCCTGAATCC TGTTAAAGTA CCCGGAGAAT GCTGCCCAGT GTGTGAAGAA 
CCAACATACA TCACAATAGG TCCACCCACG TGTGAGATTT TGGTGAACTG CACATTGACT 
GAGAGAGACT GTATGTACAG TTTCAGACTG GACCAAAACG GTTGCCGCAC TTGTCAGTGC 
AAAACTAGAG AGGAGCTGTG TACTGGCCTC ATATCAGGGT GTTCCTTGGA CTGTTCCTTC 
GGTTTCCAGA CTGATGTCCA CAACTGTGAG ATCTGTCAGT GCCGTCCAAG GCCTAAGAAA 
TGCAAACCTA TAGTATGTGA CAAGTACTGT CCATTTGGAT ACTTGAAGAA CAAGCATGGC 
TGTGATACCT GTCGCTGTAA GAAATGCCCA GAAGTTCCTT GTGGTAAGAT CTGTCCAATG 
GGCTTCCAGC AGAATAATCA TGGTTGTCTT CTCTGCAAGT GCAGAGAGAT ACCAGATTCT 
GTTATGCCAC CAGTTAAAAC AGGCTCTTGC CTGTCAATGG ATGGTCATCG TCATGAGAAA 
GAGGAGAGTT GGCATGATGG TTGCCGAGAA TGTTATTGTC TCAATGGACG GGAGATGTGT 
GCGTTGATCA CCTGCCCTGT GCCAAACTGT GGCAATCCTA CCATACATCC AGGACAATGT 
TGTCCATCAT GTCCAGATGA ATCTATTGTG CAGAAGCCAG AGCTTAGCAG TCCATCTATC 
TGCCATGCTC CAGGAGGAGA ATACTTTGTA GAAGGAGAGA CATGGAATAT TGACACCTGC 
ACACAGTGTA CATGCCATAG TGGACGCGTT CTGTGTGAGA CCGAAGTCTG CCCACCTCTT 
CTTTGTCAAA ACCCCATCCG NNNNNNNNNN NTCTGTCTAT TCATGTTTCC AGATGAACCT 
CTTCAGCCCT CCTCATCAAG CAACGAAAGC ATGCCCAGTT ACTGTAAAAA CGACGAAGGA 
GATATTTTCC TGACAGCTGA GTCCTGGAAA CCCAATGTCT GCACTAGTTG TATTTGCATG 
GATGGTGTGA TTAGCTGCTA CTCTGAATCT TGCCCTCCAG TATCCTGTGA GAGACCTGTT 
TTGAGAAAAG GACAGTGTTG CCCTTATTGT ATAGAAGATA CAATACCAAA GAGGGTGGTA 
TGTCACTTCA GTGGAAAAAC ATATGCAGAT GAGGAACGTT GGGACATCGA CAGCTGCACA 
CACTGCTACT GCCTGCAGGG GCAGACCCTT TGCTCCACTG TCAGTTGTCC TCCTCTCCCC 
TGTGTAGAAC CCATCAACGT TGAGGGAAGT TGCTGTCCCA TGTGTCCAGA GATGTATGTA 
CCTGAGCCCA CCAACGTCCC CATTGAGAAA ACAAATCATC GAGGAGATAC TGAACTAGAA 
GCACCAATTT GGCCTACACC TAGTGAAAAT GACATTGTTC ATCTCCATAG AGACATGAGT 
CATCTTCAAG CAGATTTCAG AGAAGGCATT GAATTGCACC CCAGTGAAGA CACATCTCTC 
AACTCTGTTG CCTGGGTGAC AGTTCCCATC ATGATAGCCC TAGTTCTTAT AATAGCCCTT 
CTGCTTGTCA ATCAGAAGAA GCAGTGGATA CCAGTGTCCT GTTACAGAGC CCCAACAAAG 
TCTTCTTGCC TAAACAATCA GCTGGTATAT GTGGACTGCA AGAAAGGTAC CATGGTTCAG 
GTGGATAGTT CTCAGAGGAT GCTAAGAATT GCCGACCCAG ATTCAAGATA CAGTGGATTC 
TACAGCATGC AAAAACAGAA CAACCTACAG GCAGATAATT TCTATCAAAC AGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CRIM1 ( XM_006131287.2 ) cDNA ORF clone, Pelodiscus sinensis(Chinese soft-shelled turtle) -> XP_006131349.1 Pelodiscus sinensis cysteine rich transmembrane BMP regulator 1 (chordin-like) (CRIM1), mRNA.

Price & Availability↑

CloneID	OPe36732
Clone ID Related Accession (Same CDS sequence)	XM_006131287.3 , XM_006131287.2
Accession Version	XM_006131287.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3117bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-11-02
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Product	cysteine-rich motor neuron 1 protein
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005857385.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 3, 2015 this sequence version replaced XM_006131287.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pelodiscus sinensis Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACCTGG CAGCGGTCTT GGCAGGGCGG AGGCGCCCGG GCGGCGGCTG GCACCTGGCG 
GCGGGGTGGC TGCTCTTGGT GCTGCTGGGC GCGTCCGGGA CGCGGGCGCT CGTCTGCCTC 
CCCTGCGACG AGTCCAAGTG CGAGGAGCCC AAGAGCTGCC CCGGCAGCAT CGTGCTGGGC 
ATCTGCGGCT GCTGCTTCAT GTGCGCCCGG CAGCGCAACG AGAGCTGCGG GGGCGTCTAC 
GGGCTGCACG GAGCCTGCGA CCGCGGGCTG CGCTGTGTCA TCCGCCCCCC GCTCAACGGG 
GACTCCATCA CCGAGTACGA AGTGGGCGTC TGCGAAGATG AAAATTGGGA TGATGATCAG 
CTACTTGGAT TTGAACCTTG CAATGAAAAC CTTGTAACAG GTTGCAACAT AATCAATGGG 
AAATGTGAAT GTGACACCAT TCGCACCTGT AGTAATCCAT TTGAGTTTCC AAGCCAGGAT 
ACTTGCCTGT CAGCTTTAAA AAGGATTGAA GAAGAGAAAC CTGACTGTTC CAAGGCCCGC 
TGTGAGGTCC AGTTTTCTCC TCGCTGTCCA GAAGATTCCA TCTTGATTGA AGGTTATGCT 
CCTCCAGGGG AATGCTGTCC ACTTCCAAGC CGTTGTGTTT GCAACCCTGC AGGTTGCCTG 
AGGAAAGTTT GCCAGCCAGG CTATTTGAAT ATATTGGTTT CTAAAGCATC AGGGAAACCA 
GGGGAGTGCT GTGATCTCTA CGAATGTAAA CCAGTTTTCA GTGTGGATTG CAGCACCGTG 
GAATGCCCCC CGGTTCAACA AGTTGTTTGT CCACTGGATA GCTATGAAAC CCAGGTCAGG 
TTGACAGCTG ATGGCTGCTG TACTCTTCCC ACGAGATGCG AGTGTCTCTC TGGTTTATGT 
GGTTTCCCCG TGTGCGAGGC AGGCTCCATT CCCCAGATAG TCTCACGTGG AGATGGAACA 
CCCGGCAAGT GCTGTGATGT CTTTGAATGT GTCAATGAAG TGAAGCCAGC TTGCATATTT 
AACAGTGTGG AGTATTATGA TGGTGACATG TTTCGAATGG ATGCCTGCCG TTTCTGTCGA 
TGTCAGGGTG GCGTCTCGAT TTGTTTCTCG GCCCAGTGTG GCGAGTTGCA CTGTGACAGG 
TACTATGTTC CAGAAGGAGA GTGCTGTCCA GTTTGTGAAG ATCCAGTATA TCCAGTTAAT 
AACCCTGCTG GCTGCTATGC CAATGGTCAG ATCCAAGCAC ATGGAGATCG CTGGAGAGAA 
GATGACTGCA CTTTCTGTCA GTGTATCAAT GGAGATCCCC ACTGTGTTGC AACAGCCTGT 
GGACAGAGTT GCCTGAATCC TGTTAAAGTA CCCGGAGAAT GCTGCCCAGT GTGTGAAGAA 
CCAACATACA TCACAATAGG TCCACCCACG TGTGAGATTT TGGTGAACTG CACATTGACT 
GAGAGAGACT GTATGTACAG TTTCAGACTG GACCAAAACG GTTGCCGCAC TTGTCAGTGC 
AAAACTAGAG AGGAGCTGTG TACTGGCCTC ATATCAGGGT GTTCCTTGGA CTGTTCCTTC 
GGTTTCCAGA CTGATGTCCA CAACTGTGAG ATCTGTCAGT GCCGTCCAAG GCCTAAGAAA 
TGCAAACCTA TAGTATGTGA CAAGTACTGT CCATTTGGAT ACTTGAAGAA CAAGCATGGC 
TGTGATACCT GTCGCTGTAA GAAATGCCCA GAAGTTCCTT GTGGTAAGAT CTGTCCAATG 
GGCTTCCAGC AGAATAATCA TGGTTGTCTT CTCTGCAAGT GCAGAGAGAT ACCAGATTCT 
GTTATGCCAC CAGTTAAAAC AGGCTCTTGC CTGTCAATGG ATGGTCATCG TCATGAGAAA 
GAGGAGAGTT GGCATGATGG TTGCCGAGAA TGTTATTGTC TCAATGGACG GGAGATGTGT 
GCGTTGATCA CCTGCCCTGT GCCAAACTGT GGCAATCCTA CCATACATCC AGGACAATGT 
TGTCCATCAT GTCCAGATGA ATCTATTGTG CAGAAGCCAG AGCTTAGCAG TCCATCTATC 
TGCCATGCTC CAGGAGGAGA ATACTTTGTA GAAGGAGAGA CATGGAATAT TGACACCTGC 
ACACAGTGTA CATGCCATAG TGGACGCGTT CTGTGTGAGA CCGAAGTCTG CCCACCTCTT 
CTTTGTCAAA ACCCCATCCG NNNNNNNNNN NTCTGTCTAT TCATGTTTCC AGATGAACCT 
CTTCAGCCCT CCTCATCAAG CAACGAAAGC ATGCCCAGTT ACTGTAAAAA CGACGAAGGA 
GATATTTTCC TGACAGCTGA GTCCTGGAAA CCCAATGTCT GCACTAGTTG TATTTGCATG 
GATGGTGTGA TTAGCTGCTA CTCTGAATCT TGCCCTCCAG TATCCTGTGA GAGACCTGTT 
TTGAGAAAAG GACAGTGTTG CCCTTATTGT ATAGAAGATA CAATACCAAA GAGGGTGGTA 
TGTCACTTCA GTGGAAAAAC ATATGCAGAT GAGGAACGTT GGGACATCGA CAGCTGCACA 
CACTGCTACT GCCTGCAGGG GCAGACCCTT TGCTCCACTG TCAGTTGTCC TCCTCTCCCC 
TGTGTAGAAC CCATCAACGT TGAGGGAAGT TGCTGTCCCA TGTGTCCAGA GATGTATGTA 
CCTGAGCCCA CCAACGTCCC CATTGAGAAA ACAAATCATC GAGGAGATAC TGAACTAGAA 
GCACCAATTT GGCCTACACC TAGTGAAAAT GACATTGTTC ATCTCCATAG AGACATGAGT 
CATCTTCAAG CAGATTTCAG AGAAGGCATT GAATTGCACC CCAGTGAAGA CACATCTCTC 
AACTCTGTTG CCTGGGTGAC AGTTCCCATC ATGATAGCCC TAGTTCTTAT AATAGCCCTT 
CTGCTTGTCA ATCAGAAGAA GCAGTGGATA CCAGTGTCCT GTTACAGAGC CCCAACAAAG 
TCTTCTTGCC TAAACAATCA GCTGGTATAT GTGGACTGCA AGAAAGGTAC CATGGTTCAG 
GTGGATAGTT CTCAGAGGAT GCTAAGAATT GCCGACCCAG ATTCAAGATA CAGTGGATTC 
TACAGCATGC AAAAACAGAA CAACCTACAG GCAGATAATT TCTATCAAAC AGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006131349.1
CDS	389..3505
Translation	MYLAAVLAGRRRPGGGWHLAAGWLLLVLLGASGTRALVCLPCDESKCEEPKSCPGSIVLGICGCCFMCARQRNESCGGVYGLHGACDRGLRCVIRPPLNGDSITEYEVGVCEDENWDDDQLLGFEPCNENLVTGCNIINGKCECDTIRTCSNPFEFPSQDTCLSALKRIEEEKPDCSKARCEVQFSPRCPEDSILIEGYAPPGECCPLPSRCVCNPAGCLRKVCQPGYLNILVSKASGKPGECCDLYECKPVFSVDCSTVECPPVQQVVCPLDSYETQVRLTADGCCTLPTRCECLSGLCGFPVCEAGSIPQIVSRGDGTPGKCCDVFECVNEVKPACIFNSVEYYDGDMFRMDACRFCRCQGGVSICFSAQCGELHCDRYYVPEGECCPVCEDPVYPVNNPAGCYANGQIQAHGDRWREDDCTFCQCINGDPHCVATACGQSCLNPVKVPGECCPVCEEPTYITIGPPTCEILVNCTLTERDCMYSFRLDQNGCRTCQCKTREELCTGLISGCSLDCSFGFQTDVHNCEICQCRPRPKKCKPIVCDKYCPFGYLKNKHGCDTCRCKKCPEVPCGKICPMGFQQNNHGCLLCKCREIPDSVMPPVKTGSCLSMDGHRHEKEESWHDGCRECYCLNGREMCALITCPVPNCGNPTIHPGQCCPSCPDESIVQKPELSSPSICHAPGGEYFVEGETWNIDTCTQCTCHSGRVLCETEVCPPLLCQNPInullnullnullnullnullCLFMFPDEPLQPSSSSNESMPSYCKNDEGDIFLTAESWKPNVCTSCICMDGVISCYSESCPPVSCERPVLRKGQCCPYCIEDTIPKRVVCHFSGKTYADEERWDIDSCTHCYCLQGQTLCSTVSCPPLPCVEPINVEGSCCPMCPEMYVPEPTNVPIEKTNHRGDTELEAPIWPTPSENDIVHLHRDMSHLQADFREGIELHPSEDTSLNSVAWVTVPIMIALVLIIALLLVNQKKQWIPVSCYRAPTKSSCLNNQLVYVDCKKGTMVQVDSSQRMLRIADPDSRYSGFYSMQKQNNLQADNFYQTV

Target ORF information:

RefSeq Version	XM_006131287.2
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Definition	Pelodiscus sinensis cysteine rich transmembrane BMP regulator 1 (chordin-like) (CRIM1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006131287.2

ORF Insert Sequence:

ATGTACCTGG CAGCGGTCTT GGCAGGGCGG AGGCGCCCGG GCGGCGGCTG GCACCTGGCG 
GCGGGGTGGC TGCTCTTGGT GCTGCTGGGC GCGTCCGGGA CGCGGGCGCT CGTCTGCCTC 
CCCTGCGACG AGTCCAAGTG CGAGGAGCCC AAGAGCTGCC CCGGCAGCAT CGTGCTGGGC 
ATCTGCGGCT GCTGCTTCAT GTGCGCCCGG CAGCGCAACG AGAGCTGCGG GGGCGTCTAC 
GGGCTGCACG GAGCCTGCGA CCGCGGGCTG CGCTGTGTCA TCCGCCCCCC GCTCAACGGG 
GACTCCATCA CCGAGTACGA AGTGGGCGTC TGCGAAGATG AAAATTGGGA TGATGATCAG 
CTACTTGGAT TTGAACCTTG CAATGAAAAC CTTGTAACAG GTTGCAACAT AATCAATGGG 
AAATGTGAAT GTGACACCAT TCGCACCTGT AGTAATCCAT TTGAGTTTCC AAGCCAGGAT 
ACTTGCCTGT CAGCTTTAAA AAGGATTGAA GAAGAGAAAC CTGACTGTTC CAAGGCCCGC 
TGTGAGGTCC AGTTTTCTCC TCGCTGTCCA GAAGATTCCA TCTTGATTGA AGGTTATGCT 
CCTCCAGGGG AATGCTGTCC ACTTCCAAGC CGTTGTGTTT GCAACCCTGC AGGTTGCCTG 
AGGAAAGTTT GCCAGCCAGG CTATTTGAAT ATATTGGTTT CTAAAGCATC AGGGAAACCA 
GGGGAGTGCT GTGATCTCTA CGAATGTAAA CCAGTTTTCA GTGTGGATTG CAGCACCGTG 
GAATGCCCCC CGGTTCAACA AGTTGTTTGT CCACTGGATA GCTATGAAAC CCAGGTCAGG 
TTGACAGCTG ATGGCTGCTG TACTCTTCCC ACGAGATGCG AGTGTCTCTC TGGTTTATGT 
GGTTTCCCCG TGTGCGAGGC AGGCTCCATT CCCCAGATAG TCTCACGTGG AGATGGAACA 
CCCGGCAAGT GCTGTGATGT CTTTGAATGT GTCAATGAAG TGAAGCCAGC TTGCATATTT 
AACAGTGTGG AGTATTATGA TGGTGACATG TTTCGAATGG ATGCCTGCCG TTTCTGTCGA 
TGTCAGGGTG GCGTCTCGAT TTGTTTCTCG GCCCAGTGTG GCGAGTTGCA CTGTGACAGG 
TACTATGTTC CAGAAGGAGA GTGCTGTCCA GTTTGTGAAG ATCCAGTATA TCCAGTTAAT 
AACCCTGCTG GCTGCTATGC CAATGGTCAG ATCCAAGCAC ATGGAGATCG CTGGAGAGAA 
GATGACTGCA CTTTCTGTCA GTGTATCAAT GGAGATCCCC ACTGTGTTGC AACAGCCTGT 
GGACAGAGTT GCCTGAATCC TGTTAAAGTA CCCGGAGAAT GCTGCCCAGT GTGTGAAGAA 
CCAACATACA TCACAATAGG TCCACCCACG TGTGAGATTT TGGTGAACTG CACATTGACT 
GAGAGAGACT GTATGTACAG TTTCAGACTG GACCAAAACG GTTGCCGCAC TTGTCAGTGC 
AAAACTAGAG AGGAGCTGTG TACTGGCCTC ATATCAGGGT GTTCCTTGGA CTGTTCCTTC 
GGTTTCCAGA CTGATGTCCA CAACTGTGAG ATCTGTCAGT GCCGTCCAAG GCCTAAGAAA 
TGCAAACCTA TAGTATGTGA CAAGTACTGT CCATTTGGAT ACTTGAAGAA CAAGCATGGC 
TGTGATACCT GTCGCTGTAA GAAATGCCCA GAAGTTCCTT GTGGTAAGAT CTGTCCAATG 
GGCTTCCAGC AGAATAATCA TGGTTGTCTT CTCTGCAAGT GCAGAGAGAT ACCAGATTCT 
GTTATGCCAC CAGTTAAAAC AGGCTCTTGC CTGTCAATGG ATGGTCATCG TCATGAGAAA 
GAGGAGAGTT GGCATGATGG TTGCCGAGAA TGTTATTGTC TCAATGGACG GGAGATGTGT 
GCGTTGATCA CCTGCCCTGT GCCAAACTGT GGCAATCCTA CCATACATCC AGGACAATGT 
TGTCCATCAT GTCCAGATGA ATCTATTGTG CAGAAGCCAG AGCTTAGCAG TCCATCTATC 
TGCCATGCTC CAGGAGGAGA ATACTTTGTA GAAGGAGAGA CATGGAATAT TGACACCTGC 
ACACAGTGTA CATGCCATAG TGGACGCGTT CTGTGTGAGA CCGAAGTCTG CCCACCTCTT 
CTTTGTCAAA ACCCCATCCG NNNNNNNNNN NTCTGTCTAT TCATGTTTCC AGATGAACCT 
CTTCAGCCCT CCTCATCAAG CAACGAAAGC ATGCCCAGTT ACTGTAAAAA CGACGAAGGA 
GATATTTTCC TGACAGCTGA GTCCTGGAAA CCCAATGTCT GCACTAGTTG TATTTGCATG 
GATGGTGTGA TTAGCTGCTA CTCTGAATCT TGCCCTCCAG TATCCTGTGA GAGACCTGTT 
TTGAGAAAAG GACAGTGTTG CCCTTATTGT ATAGAAGATA CAATACCAAA GAGGGTGGTA 
TGTCACTTCA GTGGAAAAAC ATATGCAGAT GAGGAACGTT GGGACATCGA CAGCTGCACA 
CACTGCTACT GCCTGCAGGG GCAGACCCTT TGCTCCACTG TCAGTTGTCC TCCTCTCCCC 
TGTGTAGAAC CCATCAACGT TGAGGGAAGT TGCTGTCCCA TGTGTCCAGA GATGTATGTA 
CCTGAGCCCA CCAACGTCCC CATTGAGAAA ACAAATCATC GAGGAGATAC TGAACTAGAA 
GCACCAATTT GGCCTACACC TAGTGAAAAT GACATTGTTC ATCTCCATAG AGACATGAGT 
CATCTTCAAG CAGATTTCAG AGAAGGCATT GAATTGCACC CCAGTGAAGA CACATCTCTC 
AACTCTGTTG CCTGGGTGAC AGTTCCCATC ATGATAGCCC TAGTTCTTAT AATAGCCCTT 
CTGCTTGTCA ATCAGAAGAA GCAGTGGATA CCAGTGTCCT GTTACAGAGC CCCAACAAAG 
TCTTCTTGCC TAAACAATCA GCTGGTATAT GTGGACTGCA AGAAAGGTAC CATGGTTCAG 
GTGGATAGTT CTCAGAGGAT GCTAAGAATT GCCGACCCAG ATTCAAGATA CAGTGGATTC 
TACAGCATGC AAAAACAGAA CAACCTACAG GCAGATAATT TCTATCAAAC AGTTTGA