PDHA1 cDNA ORF clone, Parus major(Great Tit)

Gene
Clones
gRNAs

Summary

Gene Symbol	PDHA1
Entrez Gene ID	107207275
Full Name	pyruvate dehydrogenase E1 alpha 1 subunit
Gene Type	protein-coding
Organism	Parus major(Great Tit)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015634107.2	XP_015489593.1	pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial isoform X1
XM_015634115.2	XP_015489601.1	pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial isoform X2

Pathways from BioSystems
Gene Ontology

The following PDHA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PDHA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPa157699	XM_015634107.2 Latest version!	Parus major pyruvate dehydrogenase (lipoamide) alpha 1 (PDHA1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
OPa157700	XM_015634115.2 Latest version!	Parus major pyruvate dehydrogenase (lipoamide) alpha 1 (PDHA1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PDHA1 ( XM_015634107.2 ) cDNA ORF clone, Parus major(Great Tit) -> XP_015489593.1 Parus major pyruvate dehydrogenase (lipoamide) alpha 1 (PDHA1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPa157699
Clone ID Related Accession (Same CDS sequence)	XM_015634107.2
Accession Version	XM_015634107.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1191bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-13
Organism	Parus major(Great Tit)
Product	pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031768.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Nov 4, 2016 this sequence version replaced XM_015634107.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Parus major Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCAAGA TGCTGCTGGC CGCGCTCTCC CGGGTGCTGC AGGGCCCGGC GGCCGCCGGA 
AGGACGGCCT CTCGAGTGAT GGTGGCATCA CGTAACTATG GAGACTTTGC AAGTGAAGCT 
ACGTTTGAGA TTAAGAAATG TGACCTCCAT CGGCTGGAGG AGGGGCCGGG CACCACGGCA 
GTGATGACTC GGGAGGAGGG GCTCCAGTAC TACAAGACCA TGCAGACCAT CCGGCGCATG 
GAGCTCAAGT CTGACCAGCT CTACAAACAG AAGATTATTC GTGGCTTCTG CCACTTGTAT 
GATGGTCAGG AGGCTTGCTG TGTGGGGATT GAGGCTGCCA TAAAGCCCAC GGACCACGTG 
ATAACAGCGT ACAGAGCCCA CGGCTTCACC TACACCCGAG GAGTGCCTGT CCGGGAGATT 
CTCGCGGAAC TTACAGGTCG AAAAGGAGGA TGTGCAAAGG GAAAAGGAGG ATCAATGCAT 
ATGTATACCA AAAACTTTTA CGGTGGCAAT GGTATTGTTG GTGCTCAGGT TCCTCTTGGA 
GCTGGGATTG CATTGGCCTG TAAATACTTT GGTAAAAACG AAATCGCCCT GGCCTTGTAT 
GGGGATGGTG CAGCCAATCA GGGTCAGATA TTTGAAACAT ACAACATGGC TGCCTTATGG 
AAATTGCCTT GTATTTTTAT CTGTGAGAAC AATCGGTATG GAATGGGAAC ATCAGTTGAA 
AGAGCTGCAG CCAGCACTGA CTACTACAAA AGAGGGTACT TCATTCCAGG GCTCAGGGTG 
GATGGCATGG ATATTCTCTG TGTCCGAGAA GCAATGAAGT TTGCAGCTGA GTACTGTAGA 
TCTGGAAAAG GTCCTCTTGT GATGGAGTTA CAGACATATC GTTACCATGG CCACAGTATG 
AGTGACCCTG GAATAAGCTA TCGTACTAGA GAAGAAATTC AGGAAGTGAG AAGCAAAAGT 
GATCCCATTA CTTTGCTGAA GGACAGAATG GTCAACAACA ACCTTGCTAG CGTTGAGGAA 
CTAAAGGAAA TAGATGTGGC AGTAAGGAAG GAGATCGAGG AAGCTGCTCA GTTTGCTACC 
ACTGACCCAG AGCCTCCCCT GGAAGAACTG GGCTATCACA TCTACTTCAA CGAGCCACCC 
TTTGAAGTGC GTGGCCCAAA CCAGTGGATA AAATACAAGT CTGTCAGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015489593.1
CDS	106..1296
Translation	MRKMLLAALSRVLQGPAAAGRTASRVMVASRNYGDFASEATFEIKKCDLHRLEEGPGTTAVMTREEGLQYYKTMQTIRRMELKSDQLYKQKIIRGFCHLYDGQEACCVGIEAAIKPTDHVITAYRAHGFTYTRGVPVREILAELTGRKGGCAKGKGGSMHMYTKNFYGGNGIVGAQVPLGAGIALACKYFGKNEIALALYGDGAANQGQIFETYNMAALWKLPCIFICENNRYGMGTSVERAAASTDYYKRGYFIPGLRVDGMDILCVREAMKFAAEYCRSGKGPLVMELQTYRYHGHSMSDPGISYRTREEIQEVRSKSDPITLLKDRMVNNNLASVEELKEIDVAVRKEIEEAAQFATTDPEPPLEELGYHIYFNEPPFEVRGPNQWIKYKSVS

Target ORF information:

RefSeq Version	XM_015634107.2
Organism	Parus major(Great Tit)
Definition	Parus major pyruvate dehydrogenase (lipoamide) alpha 1 (PDHA1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015634107.2

ORF Insert Sequence:

ATGCGCAAGA TGCTGCTGGC CGCGCTCTCC CGGGTGCTGC AGGGCCCGGC GGCCGCCGGA 
AGGACGGCCT CTCGAGTGAT GGTGGCATCA CGTAACTATG GAGACTTTGC AAGTGAAGCT 
ACGTTTGAGA TTAAGAAATG TGACCTCCAT CGGCTGGAGG AGGGGCCGGG CACCACGGCA 
GTGATGACTC GGGAGGAGGG GCTCCAGTAC TACAAGACCA TGCAGACCAT CCGGCGCATG 
GAGCTCAAGT CTGACCAGCT CTACAAACAG AAGATTATTC GTGGCTTCTG CCACTTGTAT 
GATGGTCAGG AGGCTTGCTG TGTGGGGATT GAGGCTGCCA TAAAGCCCAC GGACCACGTG 
ATAACAGCGT ACAGAGCCCA CGGCTTCACC TACACCCGAG GAGTGCCTGT CCGGGAGATT 
CTCGCGGAAC TTACAGGTCG AAAAGGAGGA TGTGCAAAGG GAAAAGGAGG ATCAATGCAT 
ATGTATACCA AAAACTTTTA CGGTGGCAAT GGTATTGTTG GTGCTCAGGT TCCTCTTGGA 
GCTGGGATTG CATTGGCCTG TAAATACTTT GGTAAAAACG AAATCGCCCT GGCCTTGTAT 
GGGGATGGTG CAGCCAATCA GGGTCAGATA TTTGAAACAT ACAACATGGC TGCCTTATGG 
AAATTGCCTT GTATTTTTAT CTGTGAGAAC AATCGGTATG GAATGGGAAC ATCAGTTGAA 
AGAGCTGCAG CCAGCACTGA CTACTACAAA AGAGGGTACT TCATTCCAGG GCTCAGGGTG 
GATGGCATGG ATATTCTCTG TGTCCGAGAA GCAATGAAGT TTGCAGCTGA GTACTGTAGA 
TCTGGAAAAG GTCCTCTTGT GATGGAGTTA CAGACATATC GTTACCATGG CCACAGTATG 
AGTGACCCTG GAATAAGCTA TCGTACTAGA GAAGAAATTC AGGAAGTGAG AAGCAAAAGT 
GATCCCATTA CTTTGCTGAA GGACAGAATG GTCAACAACA ACCTTGCTAG CGTTGAGGAA 
CTAAAGGAAA TAGATGTGGC AGTAAGGAAG GAGATCGAGG AAGCTGCTCA GTTTGCTACC 
ACTGACCCAG AGCCTCCCCT GGAAGAACTG GGCTATCACA TCTACTTCAA CGAGCCACCC 
TTTGAAGTGC GTGGCCCAAA CCAGTGGATA AAATACAAGT CTGTCAGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDHA1 ( XM_015634115.2 ) cDNA ORF clone, Parus major(Great Tit) -> XP_015489601.1 Parus major pyruvate dehydrogenase (lipoamide) alpha 1 (PDHA1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPa157700
Clone ID Related Accession (Same CDS sequence)	XM_015634115.2
Accession Version	XM_015634115.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1113bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-13
Organism	Parus major(Great Tit)
Product	pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031768.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Nov 4, 2016 this sequence version replaced XM_015634115.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Parus major Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGCAT CACGTAACTA TGGAGACTTT GCAAGTGAAG CTACGTTTGA GATTAAGAAA 
TGTGACCTCC ATCGGCTGGA GGAGGGGCCG GGCACCACGG CAGTGATGAC TCGGGAGGAG 
GGGCTCCAGT ACTACAAGAC CATGCAGACC ATCCGGCGCA TGGAGCTCAA GTCTGACCAG 
CTCTACAAAC AGAAGATTAT TCGTGGCTTC TGCCACTTGT ATGATGGTCA GGAGGCTTGC 
TGTGTGGGGA TTGAGGCTGC CATAAAGCCC ACGGACCACG TGATAACAGC GTACAGAGCC 
CACGGCTTCA CCTACACCCG AGGAGTGCCT GTCCGGGAGA TTCTCGCGGA ACTTACAGGT 
CGAAAAGGAG GATGTGCAAA GGGAAAAGGA GGATCAATGC ATATGTATAC CAAAAACTTT 
TACGGTGGCA ATGGTATTGT TGGTGCTCAG GTTCCTCTTG GAGCTGGGAT TGCATTGGCC 
TGTAAATACT TTGGTAAAAA CGAAATCGCC CTGGCCTTGT ATGGGGATGG TGCAGCCAAT 
CAGGGTCAGA TATTTGAAAC ATACAACATG GCTGCCTTAT GGAAATTGCC TTGTATTTTT 
ATCTGTGAGA ACAATCGGTA TGGAATGGGA ACATCAGTTG AAAGAGCTGC AGCCAGCACT 
GACTACTACA AAAGAGGGTA CTTCATTCCA GGGCTCAGGG TGGATGGCAT GGATATTCTC 
TGTGTCCGAG AAGCAATGAA GTTTGCAGCT GAGTACTGTA GATCTGGAAA AGGTCCTCTT 
GTGATGGAGT TACAGACATA TCGTTACCAT GGCCACAGTA TGAGTGACCC TGGAATAAGC 
TATCGTACTA GAGAAGAAAT TCAGGAAGTG AGAAGCAAAA GTGATCCCAT TACTTTGCTG 
AAGGACAGAA TGGTCAACAA CAACCTTGCT AGCGTTGAGG AACTAAAGGA AATAGATGTG 
GCAGTAAGGA AGGAGATCGA GGAAGCTGCT CAGTTTGCTA CCACTGACCC AGAGCCTCCC 
CTGGAAGAAC TGGGCTATCA CATCTACTTC AACGAGCCAC CCTTTGAAGT GCGTGGCCCA 
AACCAGTGGA TAAAATACAA GTCTGTCAGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015489601.1
CDS	31..1143
Translation	MVASRNYGDFASEATFEIKKCDLHRLEEGPGTTAVMTREEGLQYYKTMQTIRRMELKSDQLYKQKIIRGFCHLYDGQEACCVGIEAAIKPTDHVITAYRAHGFTYTRGVPVREILAELTGRKGGCAKGKGGSMHMYTKNFYGGNGIVGAQVPLGAGIALACKYFGKNEIALALYGDGAANQGQIFETYNMAALWKLPCIFICENNRYGMGTSVERAAASTDYYKRGYFIPGLRVDGMDILCVREAMKFAAEYCRSGKGPLVMELQTYRYHGHSMSDPGISYRTREEIQEVRSKSDPITLLKDRMVNNNLASVEELKEIDVAVRKEIEEAAQFATTDPEPPLEELGYHIYFNEPPFEVRGPNQWIKYKSVS

Target ORF information:

RefSeq Version	XM_015634115.2
Organism	Parus major(Great Tit)
Definition	Parus major pyruvate dehydrogenase (lipoamide) alpha 1 (PDHA1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015634115.2

ORF Insert Sequence:

ATGGTGGCAT CACGTAACTA TGGAGACTTT GCAAGTGAAG CTACGTTTGA GATTAAGAAA 
TGTGACCTCC ATCGGCTGGA GGAGGGGCCG GGCACCACGG CAGTGATGAC TCGGGAGGAG 
GGGCTCCAGT ACTACAAGAC CATGCAGACC ATCCGGCGCA TGGAGCTCAA GTCTGACCAG 
CTCTACAAAC AGAAGATTAT TCGTGGCTTC TGCCACTTGT ATGATGGTCA GGAGGCTTGC 
TGTGTGGGGA TTGAGGCTGC CATAAAGCCC ACGGACCACG TGATAACAGC GTACAGAGCC 
CACGGCTTCA CCTACACCCG AGGAGTGCCT GTCCGGGAGA TTCTCGCGGA ACTTACAGGT 
CGAAAAGGAG GATGTGCAAA GGGAAAAGGA GGATCAATGC ATATGTATAC CAAAAACTTT 
TACGGTGGCA ATGGTATTGT TGGTGCTCAG GTTCCTCTTG GAGCTGGGAT TGCATTGGCC 
TGTAAATACT TTGGTAAAAA CGAAATCGCC CTGGCCTTGT ATGGGGATGG TGCAGCCAAT 
CAGGGTCAGA TATTTGAAAC ATACAACATG GCTGCCTTAT GGAAATTGCC TTGTATTTTT 
ATCTGTGAGA ACAATCGGTA TGGAATGGGA ACATCAGTTG AAAGAGCTGC AGCCAGCACT 
GACTACTACA AAAGAGGGTA CTTCATTCCA GGGCTCAGGG TGGATGGCAT GGATATTCTC 
TGTGTCCGAG AAGCAATGAA GTTTGCAGCT GAGTACTGTA GATCTGGAAA AGGTCCTCTT 
GTGATGGAGT TACAGACATA TCGTTACCAT GGCCACAGTA TGAGTGACCC TGGAATAAGC 
TATCGTACTA GAGAAGAAAT TCAGGAAGTG AGAAGCAAAA GTGATCCCAT TACTTTGCTG 
AAGGACAGAA TGGTCAACAA CAACCTTGCT AGCGTTGAGG AACTAAAGGA AATAGATGTG 
GCAGTAAGGA AGGAGATCGA GGAAGCTGCT CAGTTTGCTA CCACTGACCC AGAGCCTCCC 
CTGGAAGAAC TGGGCTATCA CATCTACTTC AACGAGCCAC CCTTTGAAGT GCGTGGCCCA 
AACCAGTGGA TAAAATACAA GTCTGTCAGC TAA