LOC107451685 cDNA ORF clone, Parasteatoda tepidariorum(common house spider)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC107451685
Entrez Gene ID	107451685
Full Name	roundabout homolog 3
Gene Type	protein-coding
Organism	Parasteatoda tepidariorum(common house spider)

mRNA and Protein(s)

mRNA	Protein	Name
XM_016067885.1	XP_015923371.1	roundabout homolog 3 isoform X5
XM_016067877.1	XP_015923363.1	hemicentin-2-like isoform X4
XM_016067870.1	XP_015923356.1	hemicentin-2-like isoform X3
XM_016067870.2	XP_015923356.1	hemicentin-1 isoform X3
XM_016067877.2	XP_015923363.1	hemicentin-1 isoform X4
XM_016067864.1	XP_015923350.1	hemicentin-1 isoform X2
XM_016067855.1	XP_015923341.1	hemicentin-1 isoform X1

Pathways from BioSystems
Gene Ontology

The following LOC107451685 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC107451685 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPa120370	XM_016067885.1 Latest version!	Parasteatoda tepidariorum roundabout homolog 3 (LOC107451685), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OPa120366	XM_016067855.1 Latest version!	Parasteatoda tepidariorum roundabout homolog 3 (LOC107451685), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OPa120367	XM_016067864.1 Latest version!	Parasteatoda tepidariorum roundabout homolog 3 (LOC107451685), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OPa120368	XM_016067870.2 Latest version!	Parasteatoda tepidariorum roundabout homolog 3 (LOC107451685), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OPa120369	XM_016067877.2 Latest version!	Parasteatoda tepidariorum roundabout homolog 3 (LOC107451685), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
View Old Accession Versions >>
OPa120368	XM_016067870.1	Parasteatoda tepidariorum roundabout homolog 3-like (LOC107451685), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OPa120369	XM_016067877.1	Parasteatoda tepidariorum roundabout homolog 3-like (LOC107451685), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC107451685 ( XM_016067885.1 ) cDNA ORF clone, Parasteatoda tepidariorum(common house spider) -> XP_015923371.1 Parasteatoda tepidariorum roundabout homolog 3 (LOC107451685), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OPa120370
Clone ID Related Accession (Same CDS sequence)	XM_016067885.1
Accession Version	XM_016067885.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2880bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-14
Organism	Parasteatoda tepidariorum(common house spider)
Product	roundabout homolog 3 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018372425.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Parasteatoda tepidariorum Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCAGA AAGGTGGCTC CAGGGAAAGG CCTACGTCCA GCGTACTGAC CATCACTCCT 
AGACTAGAAG ACGATGATTC TATCTATAGA TGTGTTGTTT GGAACAGAGC TTTGAGGGAG 
GATCAAAAAC TTGAAACAAG CGTCAGTCTT AGGGTTCACT ATTCACCTAG AATAACTGTG 
GGGCCCTCTA ACCCTTTAAA CGTCCTAGAA AATACTGATG CTCATTTAAG CTGCAATGTG 
ATAGCATCAC CCCCTGTTCG TTCTGTCAGG TGGTTGAGGA ATGGAATGCT GATATCTAAC 
ACTCTCAATC ATACTATCAG AGGTGTCACA TCTGAAGACA GTGGTATTTA TAGCTGTATC 
GCTGAAAATG GCATTGGAGA ACCTACACAA GCCAACTTGG AATTATCTGT GCTTTATGGC 
CCCCAAGTGA GACTTCCAAC TTATCAGGAA GTAGCTGCTG GCGAACCTCT CATAGTTAAA 
TGTGAAGTCT TGTCTAAACC TCCTCCTCAT ATGATCCAAT GGCTGAAAGA AGGGGATCCG 
TACTTCAGGC AAACAGGAGA CACTCTACGA ATTGATAAAA TTACTGTAGA TGATGGTGGA 
CGATATATTT GCCAGGCTGC CACTTCATTC AGACCTTCAG GTTCTAACAT CCAAACTGAA 
GCTACAGGAA ATGGAACAGT TACAGTTCGA ATCAAACATA AACCTGGCCA GGCTGAAATC 
CATCCTTCTC AACCTGTAGC CATTGCTGGT CGACCGTTTA CACTCTCTTG CACAGCACAC 
CCTCTTGGAT GGCCAGTACC TGAATACAGG TGGTGGAAAG AAGGCCAGCA GAAAGAAGAC 
CTTACTCAGA AACCGAATTA TACCCTAATA TCAGCTCACA TGAGCCATGA AGGTAGATAT 
TTCTGCCAGC CTTTTAATAG TCTTGGAAGG GGTGGATCGG CATCTGTGTA TCTAACTGTG 
CATGAACCAG CATCTATCCT CATACCACTT AGACCTCAAA CCATCAAAAA GGACAAAGAA 
AGAGATTACA GTATAACATG CAGAGCTCGT GGAAAGCCCA AACCAAAAGT CAGGTGGTTA 
CAGAATGGTC AAGAAATATC TGAAGATAGC GGGCTGTTCC ATATTTCAAA TTCTGATAAT 
GTAGAGGACA ATAATGCATT CACCGTACAA AGTACTCTGT CGTTTACGGG ACCCAGTCGT 
CGAGAGAATG GCCTCACTCC TGGCGACAGA GGAAGATATA CTTGTGAATT TAATAATGGA 
TTTGGCGAGA ATGCTAGGTC TGAAATGATG CTCAGAATTG AACATTCCCC TGTGGTCCGA 
CATACGTACA ATCGAGTGGC TTTTGATGTG GGAGAGACCG CTGTTTTGCA GTGCAAAATG 
CAGGCTTACC CTAGCCCAAC ATTTGAATGG GTTTCGAGAG GACGTGTTCT GGACGAATAT 
GGGAACTATG GTACGAACAT CACAGACCTA GGCGAAGACA TATATGCTGG CGTTCTCTCT 
ATTCGAGACA TGAAGGAAGA AGACTACGGA GAGTACACGT GTAGAGCTTC CAATCAAGTT 
GGGGATGACA AGAAAACCAT TATCAGGCTT GTTAGGAAAG GTCCTCCTGA GAGGCCTACT 
CACTTAGAGT TGATAGAAGT TAGTTCAGAT AAAGTGACTT TGAGATGGCA GGAAGGATTC 
AATGGTGGTT TCGCCAACAC TGAGTTTTTG GTTACGTATC TCAATACTGA AAATGGTCGA 
CAAATGAATG AGTCCTGTCG GACTCAAAAT TTGTGCCAAA TCACTGGTCT CTCTGCAAAT 
ACAGAATATC ACTTCAAAGT TTTGGCTGTT AATCCCCGTG GTTATAGTCC ATATTCAGAA 
GAGCTGTCTG TTACAACTAA AGTGAATTTA AGAGACATGC CAAGACCAAC AGAAGCACAA 
TATGATCGTG ATACTGGCTA TCTTACATTT CATGTTGAAT TAAGTTCATT AAAATTGTTG 
GCGAAGATTG AGGCTAGACC TGTGGGATCA GAGATGTGGG TCCTACAGAC TGTGATCTCG 
GTCATTAGAC CCATAGAGAA AGTGCAGTTA AGCCCTCCTG ATGAGGGATT TTCTGATGTG 
CGTGTCAAAC TTTGTCTTGA TAGTAATGAC TCTTGGTGTG GTGATGCCAA ATTGGCAAAT 
CCTTTTGAAG AAGAGCTTCC AAATCCCAAA GATTCTAGAG CTGGTTTACC AACTGAGGCT 
GTCATGATAA GCATTGTTAT TGGAGGTGTT GTATTTTTAT TGGTTATCAT TTTAAGCATT 
TGCTGCTGTT GGAAGAAGAG AGTAAATCAC AAAGAAAAGA AAGATTTTGA GATGGAAACA 
ACAAATCCTA GACCAAAGGC TGTGACGCCA CCTTACTTCC CAGACGGGGT TGTGAACAAG 
GGATTGGAGA GCGTGACGGA CGACATGACA AAAGCAGGTA TCTACACCAC CAATACATCT 
GGCATCAATG GAGGGATCAA TGGCCATATA CCTTCTGGCA GTCACCTCAA TTCTGGAATG 
ATGTATAATC CAGATCCTGA TACTAGTCCC TGTAGTGCCC TAGATCATTC AGAACCCTGG 
TTGAAACCAA CAGACGAGCT TGGAACAGAA GGTTCCTATC AGCCTTATGA TTCTAGGTTA 
CCTAATGGTT ATTTTTACCC AGAAGATTAT CAACCTTTAA CAGAAGATTT AATGAACATT 
AAAAATAGAG ACCATATACA TTCACCATAC TACGATGTGA GTGGTCTGCC GGATCCATAC 
GCCATGGGCG AGGAAGACAA ACCACAACAC ATCTCCCTCT CGTACGACGA GAGTCATGAA 
AGTGGCTACT CCACACCTAA CTCTAGATCG AGGAGAGTCA TTCGGGAAAT AATTGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015923371.1
CDS	268..3147
Translation	MVQKGGSRERPTSSVLTITPRLEDDDSIYRCVVWNRALREDQKLETSVSLRVHYSPRITVGPSNPLNVLENTDAHLSCNVIASPPVRSVRWLRNGMLISNTLNHTIRGVTSEDSGIYSCIAENGIGEPTQANLELSVLYGPQVRLPTYQEVAAGEPLIVKCEVLSKPPPHMIQWLKEGDPYFRQTGDTLRIDKITVDDGGRYICQAATSFRPSGSNIQTEATGNGTVTVRIKHKPGQAEIHPSQPVAIAGRPFTLSCTAHPLGWPVPEYRWWKEGQQKEDLTQKPNYTLISAHMSHEGRYFCQPFNSLGRGGSASVYLTVHEPASILIPLRPQTIKKDKERDYSITCRARGKPKPKVRWLQNGQEISEDSGLFHISNSDNVEDNNAFTVQSTLSFTGPSRRENGLTPGDRGRYTCEFNNGFGENARSEMMLRIEHSPVVRHTYNRVAFDVGETAVLQCKMQAYPSPTFEWVSRGRVLDEYGNYGTNITDLGEDIYAGVLSIRDMKEEDYGEYTCRASNQVGDDKKTIIRLVRKGPPERPTHLELIEVSSDKVTLRWQEGFNGGFANTEFLVTYLNTENGRQMNESCRTQNLCQITGLSANTEYHFKVLAVNPRGYSPYSEELSVTTKVNLRDMPRPTEAQYDRDTGYLTFHVELSSLKLLAKIEARPVGSEMWVLQTVISVIRPIEKVQLSPPDEGFSDVRVKLCLDSNDSWCGDAKLANPFEEELPNPKDSRAGLPTEAVMISIVIGGVVFLLVIILSICCCWKKRVNHKEKKDFEMETTNPRPKAVTPPYFPDGVVNKGLESVTDDMTKAGIYTTNTSGINGGINGHIPSGSHLNSGMMYNPDPDTSPCSALDHSEPWLKPTDELGTEGSYQPYDSRLPNGYFYPEDYQPLTEDLMNIKNRDHIHSPYYDVSGLPDPYAMGEEDKPQHISLSYDESHESGYSTPNSRSRRVIREIIV

Target ORF information:

RefSeq Version	XM_016067885.1
Organism	Parasteatoda tepidariorum(common house spider)
Definition	Parasteatoda tepidariorum roundabout homolog 3 (LOC107451685), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016067885.1

ORF Insert Sequence:

ATGGTCCAGA AAGGTGGCTC CAGGGAAAGG CCTACGTCCA GCGTACTGAC CATCACTCCT 
AGACTAGAAG ACGATGATTC TATCTATAGA TGTGTTGTTT GGAACAGAGC TTTGAGGGAG 
GATCAAAAAC TTGAAACAAG CGTCAGTCTT AGGGTTCACT ATTCACCTAG AATAACTGTG 
GGGCCCTCTA ACCCTTTAAA CGTCCTAGAA AATACTGATG CTCATTTAAG CTGCAATGTG 
ATAGCATCAC CCCCTGTTCG TTCTGTCAGG TGGTTGAGGA ATGGAATGCT GATATCTAAC 
ACTCTCAATC ATACTATCAG AGGTGTCACA TCTGAAGACA GTGGTATTTA TAGCTGTATC 
GCTGAAAATG GCATTGGAGA ACCTACACAA GCCAACTTGG AATTATCTGT GCTTTATGGC 
CCCCAAGTGA GACTTCCAAC TTATCAGGAA GTAGCTGCTG GCGAACCTCT CATAGTTAAA 
TGTGAAGTCT TGTCTAAACC TCCTCCTCAT ATGATCCAAT GGCTGAAAGA AGGGGATCCG 
TACTTCAGGC AAACAGGAGA CACTCTACGA ATTGATAAAA TTACTGTAGA TGATGGTGGA 
CGATATATTT GCCAGGCTGC CACTTCATTC AGACCTTCAG GTTCTAACAT CCAAACTGAA 
GCTACAGGAA ATGGAACAGT TACAGTTCGA ATCAAACATA AACCTGGCCA GGCTGAAATC 
CATCCTTCTC AACCTGTAGC CATTGCTGGT CGACCGTTTA CACTCTCTTG CACAGCACAC 
CCTCTTGGAT GGCCAGTACC TGAATACAGG TGGTGGAAAG AAGGCCAGCA GAAAGAAGAC 
CTTACTCAGA AACCGAATTA TACCCTAATA TCAGCTCACA TGAGCCATGA AGGTAGATAT 
TTCTGCCAGC CTTTTAATAG TCTTGGAAGG GGTGGATCGG CATCTGTGTA TCTAACTGTG 
CATGAACCAG CATCTATCCT CATACCACTT AGACCTCAAA CCATCAAAAA GGACAAAGAA 
AGAGATTACA GTATAACATG CAGAGCTCGT GGAAAGCCCA AACCAAAAGT CAGGTGGTTA 
CAGAATGGTC AAGAAATATC TGAAGATAGC GGGCTGTTCC ATATTTCAAA TTCTGATAAT 
GTAGAGGACA ATAATGCATT CACCGTACAA AGTACTCTGT CGTTTACGGG ACCCAGTCGT 
CGAGAGAATG GCCTCACTCC TGGCGACAGA GGAAGATATA CTTGTGAATT TAATAATGGA 
TTTGGCGAGA ATGCTAGGTC TGAAATGATG CTCAGAATTG AACATTCCCC TGTGGTCCGA 
CATACGTACA ATCGAGTGGC TTTTGATGTG GGAGAGACCG CTGTTTTGCA GTGCAAAATG 
CAGGCTTACC CTAGCCCAAC ATTTGAATGG GTTTCGAGAG GACGTGTTCT GGACGAATAT 
GGGAACTATG GTACGAACAT CACAGACCTA GGCGAAGACA TATATGCTGG CGTTCTCTCT 
ATTCGAGACA TGAAGGAAGA AGACTACGGA GAGTACACGT GTAGAGCTTC CAATCAAGTT 
GGGGATGACA AGAAAACCAT TATCAGGCTT GTTAGGAAAG GTCCTCCTGA GAGGCCTACT 
CACTTAGAGT TGATAGAAGT TAGTTCAGAT AAAGTGACTT TGAGATGGCA GGAAGGATTC 
AATGGTGGTT TCGCCAACAC TGAGTTTTTG GTTACGTATC TCAATACTGA AAATGGTCGA 
CAAATGAATG AGTCCTGTCG GACTCAAAAT TTGTGCCAAA TCACTGGTCT CTCTGCAAAT 
ACAGAATATC ACTTCAAAGT TTTGGCTGTT AATCCCCGTG GTTATAGTCC ATATTCAGAA 
GAGCTGTCTG TTACAACTAA AGTGAATTTA AGAGACATGC CAAGACCAAC AGAAGCACAA 
TATGATCGTG ATACTGGCTA TCTTACATTT CATGTTGAAT TAAGTTCATT AAAATTGTTG 
GCGAAGATTG AGGCTAGACC TGTGGGATCA GAGATGTGGG TCCTACAGAC TGTGATCTCG 
GTCATTAGAC CCATAGAGAA AGTGCAGTTA AGCCCTCCTG ATGAGGGATT TTCTGATGTG 
CGTGTCAAAC TTTGTCTTGA TAGTAATGAC TCTTGGTGTG GTGATGCCAA ATTGGCAAAT 
CCTTTTGAAG AAGAGCTTCC AAATCCCAAA GATTCTAGAG CTGGTTTACC AACTGAGGCT 
GTCATGATAA GCATTGTTAT TGGAGGTGTT GTATTTTTAT TGGTTATCAT TTTAAGCATT 
TGCTGCTGTT GGAAGAAGAG AGTAAATCAC AAAGAAAAGA AAGATTTTGA GATGGAAACA 
ACAAATCCTA GACCAAAGGC TGTGACGCCA CCTTACTTCC CAGACGGGGT TGTGAACAAG 
GGATTGGAGA GCGTGACGGA CGACATGACA AAAGCAGGTA TCTACACCAC CAATACATCT 
GGCATCAATG GAGGGATCAA TGGCCATATA CCTTCTGGCA GTCACCTCAA TTCTGGAATG 
ATGTATAATC CAGATCCTGA TACTAGTCCC TGTAGTGCCC TAGATCATTC AGAACCCTGG 
TTGAAACCAA CAGACGAGCT TGGAACAGAA GGTTCCTATC AGCCTTATGA TTCTAGGTTA 
CCTAATGGTT ATTTTTACCC AGAAGATTAT CAACCTTTAA CAGAAGATTT AATGAACATT 
AAAAATAGAG ACCATATACA TTCACCATAC TACGATGTGA GTGGTCTGCC GGATCCATAC 
GCCATGGGCG AGGAAGACAA ACCACAACAC ATCTCCCTCT CGTACGACGA GAGTCATGAA 
AGTGGCTACT CCACACCTAA CTCTAGATCG AGGAGAGTCA TTCGGGAAAT AATTGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107451685 ( XM_016067855.1 ) cDNA ORF clone, Parasteatoda tepidariorum(common house spider) -> XP_015923341.1 Parasteatoda tepidariorum roundabout homolog 3 (LOC107451685), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPa120366
Clone ID Related Accession (Same CDS sequence)	XM_016067855.1
Accession Version	XM_016067855.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3603bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-14
Organism	Parasteatoda tepidariorum(common house spider)
Product	hemicentin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018372425.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Parasteatoda tepidariorum Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCAATC GAACTGCAGG CCGGTGCTGT CTTCTGAGTT CTGTTTTATT CTTGGATTTA 
TTCCTTCGAA TATTGTTTTT GTTTGTGGAT GGATGTCGTT TCTTGGATTG GTGTTCTGAA 
GTTAGTGTTT ATTTGCAAGT GCGCGTTCTG TTAGGAATAT TTAAAAACAT GTTGGTGGAC 
CTGTGTACTA TGGATATAAT GATCGCGCGG TTGTGCAATA TTGTGAGGAT TCTAGATATT 
ACCACAGGTT GTTGCTCTGG GCAAGTTATG CTAGGTGATA GCAGGGACGT AGTACAAGGT 
GAAAGTTTAC GACTTCCCTG TAGATTTCAT TCAGCCTTAG GATCAAGTAC TACGCTCTAC 
TTTTGGAGTA GAGTGAATAA AGATGGTAAA GACAATGCAG CTATCAATGC TGCGGCATTG 
GACCCTCATT ACAATATAGA CTTCAGCCCT CAAGAGGGCA AATACGATCT GCTCATATCT 
CAAGCGAATT ATGACAAAGA CAATGGAAAG TTCGAATGCT GGTTGAAGGA AGGGGGCAGC 
GGAGTTGACA TCTATAGTGT TTCTTATGTC GTAACTGTTT TAATTGTTCC TGGGGAACCT 
AGAATAACTC CACCAAAGCC CATAGCCAGG GAAGGAGAGT CCACAGCTCT TACTTGCTCA 
AGTGAAGGTG GAAGCCCAGA TCCTACCATC CGCTGGTACA GAGATGGCGT CCTACTTCAA 
GGCATGGTCC AGAAAGGTGG CTCCAGGGAA AGGCCTACGT CCAGCGTACT GACCATCACT 
CCTAGACTAG AAGACGATGA TTCTATCTAT AGATGTGTTG TTTGGAACAG AGCTTTGAGG 
GAGGATCAAA AACTTGAAAC AAGCGTCAGT CTTAGGGTTC ACTATTCACC TAGAATAACT 
GTGGGGCCCT CTAACCCTTT AAACGTCCTA GAAAATACTG ATGCTCATTT AAGCTGCAAT 
GTGATAGCAT CACCCCCTGT TCGTTCTGTC AGGTGGTTGA GGAATGGAAT GCTGATATCT 
AACACTCTCA ATCATACTAT CAGAGGTGTC ACATCTGAAG ACAGTGGTAT TTATAGCTGT 
ATCGCTGAAA ATGGCATTGG AGAACCTACA CAAGCCAACT TGGAATTATC TGTGCTTTAT 
GGCCCCCAAG TGAGACTTCC AACTTATCAG GAAGTAGCTG CTGGCGAACC TCTCATAGTT 
AAATGTGAAG TCTTGTCTAA ACCTCCTCCT CATATGATCC AATGGCTGAA AGAAGGGGAT 
CCGTACTTCA GGCAAACAGG AGACACTCTA CGAATTGATA AAATTACTGT AGATGATGGT 
GGACGATATA TTTGCCAGGC TGCCACTTCA TTCAGACCTT CAGGTTCTAA CATCCAAACT 
GAAGCTACAG GAAATGGAAC AGTTACAGTT CGAATCAAAC ATAAACCTGG CCAGGCTGAA 
ATCCATCCTT CTCAACCTGT AGCCATTGCT GGTCGACCGT TTACACTCTC TTGCACAGCA 
CACCCTCTTG GATGGCCAGT ACCTGAATAC AGGTGGTGGA AAGAAGGCCA GCAGAAAGAA 
GACCTTACTC AGAAACCGAA TTATACCCTA ATATCAGCTC ACATGAGCCA TGAAGGTAGA 
TATTTCTGCC AGCCTTTTAA TAGTCTTGGA AGGGGTGGAT CGGCATCTGT GTATCTAACT 
GTGCATGAAC CAGCATCTAT CCTCATACCA CTTAGACCTC AAACCATCAA AAAGGACAAA 
GAAAGAGATT ACAGTATAAC ATGCAGAGCT CGTGGAAAGC CCAAACCAAA AGTCAGGTGG 
TTACAGAATG GTCAAGAAAT ATCTGAAGAT AGCGGGCTGT TCCATATTTC AAATTCTGAT 
AATGTAGAGG ACAATAATGC ATTCACCGTA CAAAGTACTC TGTCGTTTAC GGGACCCAGT 
CGTCGAGAGA ATGGCCTCAC TCCTGGCGAC AGAGGAAGAT ATACTTGTGA ATTTAATAAT 
GGATTTGGCG AGAATGCTAG GTCTGAAATG ATGCTCAGAA TTGAACATTC CCCTGTGGTC 
CGACATACGT ACAATCGAGT GGCTTTTGAT GTGGGAGAGA CCGCTGTTTT GCAGTGCAAA 
ATGCAGGCTT ACCCTAGCCC AACATTTGAA TGGGTTTCGA GAGGACGTGT TCTGGACGAA 
TATGGGAACT ATGGTACGAA CATCACAGAC CTAGGCGAAG ACATATATGC TGGCGTTCTC 
TCTATTCGAG ACATGAAGGA AGAAGACTAC GGAGAGTACA CGTGTAGAGC TTCCAATCAA 
GTTGGGGATG ACAAGAAAAC CATTATCAGG CTTGTTAGGA AAGGTCCTCC TGAGAGGCCT 
ACTCACTTAG AGTTGATAGA AGTTAGTTCA GATAAAGTGA CTTTGAGATG GCAGGAAGGA 
TTCAATGGTG GTTTCGCCAA CACTGAGTTT TTGGTTACGT ATCTCAATAC TGAAAATGGT 
CGACAAATGA ATGAGTCCTG TCGGACTCAA AATTTGTGCC AAATCACTGG TCTCTCTGCA 
AATACAGAAT ATCACTTCAA AGTTTTGGCT GTTAATCCCC GTGGTTATAG TCCATATTCA 
GAAGAGCTGT CTGTTACAAC TAAAGTGAAT TTAAGAGACA TGCCAAGACC AACAGAAGCA 
CAATATGATC GTGATACTGG CTATCTTACA TTTCATGTTG AATTAAGTTC ATTAAAATTG 
TTGGCGAAGA TTGAGGCTAG ACCTGTGGGA TCAGAGATGT GGGTCCTACA GACTGTGATC 
TCGGTCATTA GACCCATAGA GAAAGTGCAG TTAAGCCCTC CTGATGAGGG ATTTTCTGAT 
GTGCGTGTCA AACTTTGTCT TGATAGTAAT GACTCTTGGT GTGGTGATGC CAAATTGGCA 
AATCCTTTTG AAGAAGAGCT TCCAAATCCC AAAGATTCTA GAGCTGGTTT ACCAACTGAG 
GCTGTCATGA TAAGCATTGT TATTGGAGGT GTTGTATTTT TATTGGTTAT CATTTTAAGC 
ATTTGCTGCT GTTGGAAGAA GAGAGTAAAT CACAAAGAAA AGAAAGATTT TGAGATGGAA 
ACAACAAATC CTAGACCAAA GGCTGTGACG CCACCTTACT TCCCAGACGG GGTTGTGAAC 
AAGGGATTGG AGAGCGTGAC GGACGACATG ACAAAAGCAG GTATCTACAC CACCAATACA 
TCTGGCATCA ATGGAGGGAT CAATGGCCAT ATACCTTCTG GCAGTCACCT CAATTCTGGA 
ATGATGTATA ATCCAGATCC TGATACTAGT CCCTGTAGTG CCCTAGATCA TTCAGAACCC 
TGGTTGAAAC CAACAGACGA GCTTGGAACA GAAGGTTCCT ATCAGCCTTA TGATTCTAGG 
TTACCTAATG GTTATTTTTA CCCAGAAGAT TATCAACCTT TAACAGAAGA TTTAATGAAC 
ATTAAAAATA GAGACCATAT ACATTCACCA TACTACGATG TGAGTGGTCT GCCGGATCCA 
TACGCCATGG GCGAGGAAGA CAAACCACAA CACATCTCCC TCTCGTACGA CGAGAGTCAT 
GAAAGTGGCT ACTCCACACC TAACTCTAGA TCGAGGAGAG TCATTCGGGA AATAATTGTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015923341.1
CDS	121..3723
Translation	MTNRTAGRCCLLSSVLFLDLFLRILFLFVDGCRFLDWCSEVSVYLQVRVLLGIFKNMLVDLCTMDIMIARLCNIVRILDITTGCCSGQVMLGDSRDVVQGESLRLPCRFHSALGSSTTLYFWSRVNKDGKDNAAINAAALDPHYNIDFSPQEGKYDLLISQANYDKDNGKFECWLKEGGSGVDIYSVSYVVTVLIVPGEPRITPPKPIAREGESTALTCSSEGGSPDPTIRWYRDGVLLQGMVQKGGSRERPTSSVLTITPRLEDDDSIYRCVVWNRALREDQKLETSVSLRVHYSPRITVGPSNPLNVLENTDAHLSCNVIASPPVRSVRWLRNGMLISNTLNHTIRGVTSEDSGIYSCIAENGIGEPTQANLELSVLYGPQVRLPTYQEVAAGEPLIVKCEVLSKPPPHMIQWLKEGDPYFRQTGDTLRIDKITVDDGGRYICQAATSFRPSGSNIQTEATGNGTVTVRIKHKPGQAEIHPSQPVAIAGRPFTLSCTAHPLGWPVPEYRWWKEGQQKEDLTQKPNYTLISAHMSHEGRYFCQPFNSLGRGGSASVYLTVHEPASILIPLRPQTIKKDKERDYSITCRARGKPKPKVRWLQNGQEISEDSGLFHISNSDNVEDNNAFTVQSTLSFTGPSRRENGLTPGDRGRYTCEFNNGFGENARSEMMLRIEHSPVVRHTYNRVAFDVGETAVLQCKMQAYPSPTFEWVSRGRVLDEYGNYGTNITDLGEDIYAGVLSIRDMKEEDYGEYTCRASNQVGDDKKTIIRLVRKGPPERPTHLELIEVSSDKVTLRWQEGFNGGFANTEFLVTYLNTENGRQMNESCRTQNLCQITGLSANTEYHFKVLAVNPRGYSPYSEELSVTTKVNLRDMPRPTEAQYDRDTGYLTFHVELSSLKLLAKIEARPVGSEMWVLQTVISVIRPIEKVQLSPPDEGFSDVRVKLCLDSNDSWCGDAKLANPFEEELPNPKDSRAGLPTEAVMISIVIGGVVFLLVIILSICCCWKKRVNHKEKKDFEMETTNPRPKAVTPPYFPDGVVNKGLESVTDDMTKAGIYTTNTSGINGGINGHIPSGSHLNSGMMYNPDPDTSPCSALDHSEPWLKPTDELGTEGSYQPYDSRLPNGYFYPEDYQPLTEDLMNIKNRDHIHSPYYDVSGLPDPYAMGEEDKPQHISLSYDESHESGYSTPNSRSRRVIREIIV

Target ORF information:

RefSeq Version	XM_016067855.1
Organism	Parasteatoda tepidariorum(common house spider)
Definition	Parasteatoda tepidariorum roundabout homolog 3 (LOC107451685), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016067855.1

ORF Insert Sequence:

ATGACCAATC GAACTGCAGG CCGGTGCTGT CTTCTGAGTT CTGTTTTATT CTTGGATTTA 
TTCCTTCGAA TATTGTTTTT GTTTGTGGAT GGATGTCGTT TCTTGGATTG GTGTTCTGAA 
GTTAGTGTTT ATTTGCAAGT GCGCGTTCTG TTAGGAATAT TTAAAAACAT GTTGGTGGAC 
CTGTGTACTA TGGATATAAT GATCGCGCGG TTGTGCAATA TTGTGAGGAT TCTAGATATT 
ACCACAGGTT GTTGCTCTGG GCAAGTTATG CTAGGTGATA GCAGGGACGT AGTACAAGGT 
GAAAGTTTAC GACTTCCCTG TAGATTTCAT TCAGCCTTAG GATCAAGTAC TACGCTCTAC 
TTTTGGAGTA GAGTGAATAA AGATGGTAAA GACAATGCAG CTATCAATGC TGCGGCATTG 
GACCCTCATT ACAATATAGA CTTCAGCCCT CAAGAGGGCA AATACGATCT GCTCATATCT 
CAAGCGAATT ATGACAAAGA CAATGGAAAG TTCGAATGCT GGTTGAAGGA AGGGGGCAGC 
GGAGTTGACA TCTATAGTGT TTCTTATGTC GTAACTGTTT TAATTGTTCC TGGGGAACCT 
AGAATAACTC CACCAAAGCC CATAGCCAGG GAAGGAGAGT CCACAGCTCT TACTTGCTCA 
AGTGAAGGTG GAAGCCCAGA TCCTACCATC CGCTGGTACA GAGATGGCGT CCTACTTCAA 
GGCATGGTCC AGAAAGGTGG CTCCAGGGAA AGGCCTACGT CCAGCGTACT GACCATCACT 
CCTAGACTAG AAGACGATGA TTCTATCTAT AGATGTGTTG TTTGGAACAG AGCTTTGAGG 
GAGGATCAAA AACTTGAAAC AAGCGTCAGT CTTAGGGTTC ACTATTCACC TAGAATAACT 
GTGGGGCCCT CTAACCCTTT AAACGTCCTA GAAAATACTG ATGCTCATTT AAGCTGCAAT 
GTGATAGCAT CACCCCCTGT TCGTTCTGTC AGGTGGTTGA GGAATGGAAT GCTGATATCT 
AACACTCTCA ATCATACTAT CAGAGGTGTC ACATCTGAAG ACAGTGGTAT TTATAGCTGT 
ATCGCTGAAA ATGGCATTGG AGAACCTACA CAAGCCAACT TGGAATTATC TGTGCTTTAT 
GGCCCCCAAG TGAGACTTCC AACTTATCAG GAAGTAGCTG CTGGCGAACC TCTCATAGTT 
AAATGTGAAG TCTTGTCTAA ACCTCCTCCT CATATGATCC AATGGCTGAA AGAAGGGGAT 
CCGTACTTCA GGCAAACAGG AGACACTCTA CGAATTGATA AAATTACTGT AGATGATGGT 
GGACGATATA TTTGCCAGGC TGCCACTTCA TTCAGACCTT CAGGTTCTAA CATCCAAACT 
GAAGCTACAG GAAATGGAAC AGTTACAGTT CGAATCAAAC ATAAACCTGG CCAGGCTGAA 
ATCCATCCTT CTCAACCTGT AGCCATTGCT GGTCGACCGT TTACACTCTC TTGCACAGCA 
CACCCTCTTG GATGGCCAGT ACCTGAATAC AGGTGGTGGA AAGAAGGCCA GCAGAAAGAA 
GACCTTACTC AGAAACCGAA TTATACCCTA ATATCAGCTC ACATGAGCCA TGAAGGTAGA 
TATTTCTGCC AGCCTTTTAA TAGTCTTGGA AGGGGTGGAT CGGCATCTGT GTATCTAACT 
GTGCATGAAC CAGCATCTAT CCTCATACCA CTTAGACCTC AAACCATCAA AAAGGACAAA 
GAAAGAGATT ACAGTATAAC ATGCAGAGCT CGTGGAAAGC CCAAACCAAA AGTCAGGTGG 
TTACAGAATG GTCAAGAAAT ATCTGAAGAT AGCGGGCTGT TCCATATTTC AAATTCTGAT 
AATGTAGAGG ACAATAATGC ATTCACCGTA CAAAGTACTC TGTCGTTTAC GGGACCCAGT 
CGTCGAGAGA ATGGCCTCAC TCCTGGCGAC AGAGGAAGAT ATACTTGTGA ATTTAATAAT 
GGATTTGGCG AGAATGCTAG GTCTGAAATG ATGCTCAGAA TTGAACATTC CCCTGTGGTC 
CGACATACGT ACAATCGAGT GGCTTTTGAT GTGGGAGAGA CCGCTGTTTT GCAGTGCAAA 
ATGCAGGCTT ACCCTAGCCC AACATTTGAA TGGGTTTCGA GAGGACGTGT TCTGGACGAA 
TATGGGAACT ATGGTACGAA CATCACAGAC CTAGGCGAAG ACATATATGC TGGCGTTCTC 
TCTATTCGAG ACATGAAGGA AGAAGACTAC GGAGAGTACA CGTGTAGAGC TTCCAATCAA 
GTTGGGGATG ACAAGAAAAC CATTATCAGG CTTGTTAGGA AAGGTCCTCC TGAGAGGCCT 
ACTCACTTAG AGTTGATAGA AGTTAGTTCA GATAAAGTGA CTTTGAGATG GCAGGAAGGA 
TTCAATGGTG GTTTCGCCAA CACTGAGTTT TTGGTTACGT ATCTCAATAC TGAAAATGGT 
CGACAAATGA ATGAGTCCTG TCGGACTCAA AATTTGTGCC AAATCACTGG TCTCTCTGCA 
AATACAGAAT ATCACTTCAA AGTTTTGGCT GTTAATCCCC GTGGTTATAG TCCATATTCA 
GAAGAGCTGT CTGTTACAAC TAAAGTGAAT TTAAGAGACA TGCCAAGACC AACAGAAGCA 
CAATATGATC GTGATACTGG CTATCTTACA TTTCATGTTG AATTAAGTTC ATTAAAATTG 
TTGGCGAAGA TTGAGGCTAG ACCTGTGGGA TCAGAGATGT GGGTCCTACA GACTGTGATC 
TCGGTCATTA GACCCATAGA GAAAGTGCAG TTAAGCCCTC CTGATGAGGG ATTTTCTGAT 
GTGCGTGTCA AACTTTGTCT TGATAGTAAT GACTCTTGGT GTGGTGATGC CAAATTGGCA 
AATCCTTTTG AAGAAGAGCT TCCAAATCCC AAAGATTCTA GAGCTGGTTT ACCAACTGAG 
GCTGTCATGA TAAGCATTGT TATTGGAGGT GTTGTATTTT TATTGGTTAT CATTTTAAGC 
ATTTGCTGCT GTTGGAAGAA GAGAGTAAAT CACAAAGAAA AGAAAGATTT TGAGATGGAA 
ACAACAAATC CTAGACCAAA GGCTGTGACG CCACCTTACT TCCCAGACGG GGTTGTGAAC 
AAGGGATTGG AGAGCGTGAC GGACGACATG ACAAAAGCAG GTATCTACAC CACCAATACA 
TCTGGCATCA ATGGAGGGAT CAATGGCCAT ATACCTTCTG GCAGTCACCT CAATTCTGGA 
ATGATGTATA ATCCAGATCC TGATACTAGT CCCTGTAGTG CCCTAGATCA TTCAGAACCC 
TGGTTGAAAC CAACAGACGA GCTTGGAACA GAAGGTTCCT ATCAGCCTTA TGATTCTAGG 
TTACCTAATG GTTATTTTTA CCCAGAAGAT TATCAACCTT TAACAGAAGA TTTAATGAAC 
ATTAAAAATA GAGACCATAT ACATTCACCA TACTACGATG TGAGTGGTCT GCCGGATCCA 
TACGCCATGG GCGAGGAAGA CAAACCACAA CACATCTCCC TCTCGTACGA CGAGAGTCAT 
GAAAGTGGCT ACTCCACACC TAACTCTAGA TCGAGGAGAG TCATTCGGGA AATAATTGTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107451685 ( XM_016067864.1 ) cDNA ORF clone, Parasteatoda tepidariorum(common house spider) -> XP_015923350.1 Parasteatoda tepidariorum roundabout homolog 3 (LOC107451685), transcript variant X2, mRNA.

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CloneID	OPa120367
Clone ID Related Accession (Same CDS sequence)	XM_016067864.1
Accession Version	XM_016067864.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3591bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-14
Organism	Parasteatoda tepidariorum(common house spider)
Product	hemicentin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018372425.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Parasteatoda tepidariorum Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCAATC GAACTGCAGG CCGGTGCTGT CTTCTGAGTT CTGTTTTATT CTTGGATTTA 
TTCCTTCGAA TATTGTTTTT GTTTGTGGAT GGATGTCGTT TCTTGGATTG GTGTTCTGAA 
GTTAGTGTTT ATTTGCAAGT GCGCGTTCTG TTAGGAATAT TTAAAAACAT GTTGGTGGAC 
CTGTGTACTA TGGATATAAT GATCGCGCGG TTGTGCAATA TTGTGAGGAT TCTAGATATT 
ACCACAGGTT GTTGCTCTGG GCAAGTTATG CTAGGTGATA GCAGGGACGT AGTACAAGGT 
GAAAGTTTAC GACTTCCCTG TAGATTTCAT TCAGCCTTAG GATCAAGTAC TACGCTCTAC 
TTTTGGAGTA GAGTGAATAA AGATGGTAAA GACAATGCAG CTATCAATGC TGCGGCATTG 
GACCCTCATT ACAATATAGA CTTCAGCCCT CAAGAGGGCA AATACGATCT GCTCATATCT 
CAAGCGAATT ATGACAAAGA CAATGGAAAG TTCGAATGCT GGTTGAAGGA AGGGGGCAGC 
GGAGTTGACA TCTATAGTGT TTCTTATGTC GTAACTGTTT TAATTGTTCC TGGGGAACCT 
AGAATAACTC CACCAAAGCC CATAGCCAGG GAAGGAGAGT CCACAGCTCT TACTTGCTCA 
AGTGAAGGTG GAAGCCCAGA TCCTACCATC CGCTGGTACA GAGATGGCGT CCTACTTCAA 
GGCATGGTCC AGAAAGGTGG CTCCAGGGAA AGGCCTACGT CCAGCGTACT GACCATCACT 
CCTAGACTAG AAGACGATGA TTCTATCTAT AGATGTGTTG TTTGGAACAG AGCTTTGAGG 
GAGGATCAAA AACTTGAAAC AAGCGTCAGT CTTAGGGTTC ACTATTCACC TAGAATAACT 
GTGGGGCCCT CTAACCCTTT AAACGTCCTA GAAAATACTG ATGCTCATTT AAGCTGCAAT 
GTGATAGCAT CACCCCCTGT TCGTTCTGTC AGGTGGTTGA GGAATGGAAT GCTGATATCT 
AACACTCTCA ATCATACTAT CAGAGGTGTC ACATCTGAAG ACAGTGGTAT TTATAGCTGT 
ATCGCTGAAA ATGGCATTGG AGAACCTACA CAAGCCAACT TGGAATTATC TGTGCTTTAT 
GGCCCCCAAG TGAGACTTCC AACTTATCAG GAAGTAGCTG CTGGCGAACC TCTCATAGTT 
AAATGTGAAG TCTTGTCTAA ACCTCCTCCT CATATGATCC AATGGCTGAA AGAAGGGGAT 
CCGTACTTCA GGCAAACAGG AGACACTCTA CGAATTGATA AAATTACTGT AGATGATGGT 
GGACGATATA TTTGCCAGGC TGCCACTTCA TTCAGACCTT CAGGTTCTAA CATCCAAACT 
GAAGCTACAG GAAATGGAAC AGTTACAGTT CGAATCAAAC ATAAACCTGG CCAGGCTGAA 
ATCCATCCTT CTCAACCTGT AGCCATTGCT GGTCGACCGT TTACACTCTC TTGCACAGCA 
CACCCTCTTG GATGGCCAGT ACCTGAATAC AGGTGGTGGA AAGAAGGCCA GCAGAAAGAA 
GACCTTACTC AGAAACCGAA TTATACCCTA ATATCAGCTC ACATGAGCCA TGAAGGTAGA 
TATTTCTGCC AGCCTTTTAA TAGTCTTGGA AGGGGTGGAT CGGCATCTGT GTATCTAACT 
GTGCATGAAC CAGCATCTAT CCTCATACCA CTTAGACCTC AAACCATCAA AAAGGACAAA 
GAAAGAGATT ACAGTATAAC ATGCAGAGCT CGTGGAAAGC CCAAACCAAA AGTCAGGTGG 
TTACAGAATG GTCAAGAAAT ATCTGAAGAT AGCGGGCTGT TCCATATTTC AAATTCTGAT 
AATGTAGAGG ACAATAATGC ATTCACCGTA CAAAGTACTC TGTCGTTTAC GGGACCCAGT 
CGTCGAGAGA ATGGCCTCAC TCCTGGCGAC AGAGGAAGAT ATACTTGTGA ATTTAATAAT 
GGATTTGGCG AGAATGCTAG GTCTGAAATG ATGCTCAGAA TTGAACATTC CCCTGTGGTC 
CGACATACGT ACAATCGAGT GGCTTTTGAT GTGGGAGAGA CCGCTGTTTT GCAGTGCAAA 
ATGCAGGCTT ACCCTAGCCC AACATTTGAA TGGGTTTCGA GAGGACGTGT TCTGGACGAA 
TATGGGAACT ATGGTACGAA CATCACAGAC CTAGGCGAAG ACATATATGC TGGCGTTCTC 
TCTATTCGAG ACATGAAGGA AGAAGACTAC GGAGAGTACA CGTGTAGAGC TTCCAATCAA 
GTTGGGGATG ACAAGAAAAC CATTATCAGG CTTGTTAGGA AAGGTCCTCC TGAGAGGCCT 
ACTCACTTAG AGTTGATAGA AGTTAGTTCA GATAAAGTGA CTTTGAGATG GCAGGAAGGA 
TTCAATGGTG GTTTCGCCAA CACTGAGTTT TTGGTTACGT ATCTCAATAC TGAAAATGGT 
CGACAAATGA ATGAGTCCTG TCGGACTCAA AATTTGTGCC AAATCACTGG TCTCTCTGCA 
AATACAGAAT ATCACTTCAA AGTTTTGGCT GTTAATCCCC GTGGTTATAG TCCATATTCA 
GAAGAGCTGT CTGTTACAAC TAAAGACATG CCAAGACCAA CAGAAGCACA ATATGATCGT 
GATACTGGCT ATCTTACATT TCATGTTGAA TTAAGTTCAT TAAAATTGTT GGCGAAGATT 
GAGGCTAGAC CTGTGGGATC AGAGATGTGG GTCCTACAGA CTGTGATCTC GGTCATTAGA 
CCCATAGAGA AAGTGCAGTT AAGCCCTCCT GATGAGGGAT TTTCTGATGT GCGTGTCAAA 
CTTTGTCTTG ATAGTAATGA CTCTTGGTGT GGTGATGCCA AATTGGCAAA TCCTTTTGAA 
GAAGAGCTTC CAAATCCCAA AGATTCTAGA GCTGGTTTAC CAACTGAGGC TGTCATGATA 
AGCATTGTTA TTGGAGGTGT TGTATTTTTA TTGGTTATCA TTTTAAGCAT TTGCTGCTGT 
TGGAAGAAGA GAGTAAATCA CAAAGAAAAG AAAGATTTTG AGATGGAAAC AACAAATCCT 
AGACCAAAGG CTGTGACGCC ACCTTACTTC CCAGACGGGG TTGTGAACAA GGGATTGGAG 
AGCGTGACGG ACGACATGAC AAAAGCAGGT ATCTACACCA CCAATACATC TGGCATCAAT 
GGAGGGATCA ATGGCCATAT ACCTTCTGGC AGTCACCTCA ATTCTGGAAT GATGTATAAT 
CCAGATCCTG ATACTAGTCC CTGTAGTGCC CTAGATCATT CAGAACCCTG GTTGAAACCA 
ACAGACGAGC TTGGAACAGA AGGTTCCTAT CAGCCTTATG ATTCTAGGTT ACCTAATGGT 
TATTTTTACC CAGAAGATTA TCAACCTTTA ACAGAAGATT TAATGAACAT TAAAAATAGA 
GACCATATAC ATTCACCATA CTACGATGTG AGTGGTCTGC CGGATCCATA CGCCATGGGC 
GAGGAAGACA AACCACAACA CATCTCCCTC TCGTACGACG AGAGTCATGA AAGTGGCTAC 
TCCACACCTA ACTCTAGATC GAGGAGAGTC ATTCGGGAAA TAATTGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015923350.1
CDS	121..3711
Translation	MTNRTAGRCCLLSSVLFLDLFLRILFLFVDGCRFLDWCSEVSVYLQVRVLLGIFKNMLVDLCTMDIMIARLCNIVRILDITTGCCSGQVMLGDSRDVVQGESLRLPCRFHSALGSSTTLYFWSRVNKDGKDNAAINAAALDPHYNIDFSPQEGKYDLLISQANYDKDNGKFECWLKEGGSGVDIYSVSYVVTVLIVPGEPRITPPKPIAREGESTALTCSSEGGSPDPTIRWYRDGVLLQGMVQKGGSRERPTSSVLTITPRLEDDDSIYRCVVWNRALREDQKLETSVSLRVHYSPRITVGPSNPLNVLENTDAHLSCNVIASPPVRSVRWLRNGMLISNTLNHTIRGVTSEDSGIYSCIAENGIGEPTQANLELSVLYGPQVRLPTYQEVAAGEPLIVKCEVLSKPPPHMIQWLKEGDPYFRQTGDTLRIDKITVDDGGRYICQAATSFRPSGSNIQTEATGNGTVTVRIKHKPGQAEIHPSQPVAIAGRPFTLSCTAHPLGWPVPEYRWWKEGQQKEDLTQKPNYTLISAHMSHEGRYFCQPFNSLGRGGSASVYLTVHEPASILIPLRPQTIKKDKERDYSITCRARGKPKPKVRWLQNGQEISEDSGLFHISNSDNVEDNNAFTVQSTLSFTGPSRRENGLTPGDRGRYTCEFNNGFGENARSEMMLRIEHSPVVRHTYNRVAFDVGETAVLQCKMQAYPSPTFEWVSRGRVLDEYGNYGTNITDLGEDIYAGVLSIRDMKEEDYGEYTCRASNQVGDDKKTIIRLVRKGPPERPTHLELIEVSSDKVTLRWQEGFNGGFANTEFLVTYLNTENGRQMNESCRTQNLCQITGLSANTEYHFKVLAVNPRGYSPYSEELSVTTKDMPRPTEAQYDRDTGYLTFHVELSSLKLLAKIEARPVGSEMWVLQTVISVIRPIEKVQLSPPDEGFSDVRVKLCLDSNDSWCGDAKLANPFEEELPNPKDSRAGLPTEAVMISIVIGGVVFLLVIILSICCCWKKRVNHKEKKDFEMETTNPRPKAVTPPYFPDGVVNKGLESVTDDMTKAGIYTTNTSGINGGINGHIPSGSHLNSGMMYNPDPDTSPCSALDHSEPWLKPTDELGTEGSYQPYDSRLPNGYFYPEDYQPLTEDLMNIKNRDHIHSPYYDVSGLPDPYAMGEEDKPQHISLSYDESHESGYSTPNSRSRRVIREIIV

Target ORF information:

RefSeq Version	XM_016067864.1
Organism	Parasteatoda tepidariorum(common house spider)
Definition	Parasteatoda tepidariorum roundabout homolog 3 (LOC107451685), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016067864.1

ORF Insert Sequence:

ATGACCAATC GAACTGCAGG CCGGTGCTGT CTTCTGAGTT CTGTTTTATT CTTGGATTTA 
TTCCTTCGAA TATTGTTTTT GTTTGTGGAT GGATGTCGTT TCTTGGATTG GTGTTCTGAA 
GTTAGTGTTT ATTTGCAAGT GCGCGTTCTG TTAGGAATAT TTAAAAACAT GTTGGTGGAC 
CTGTGTACTA TGGATATAAT GATCGCGCGG TTGTGCAATA TTGTGAGGAT TCTAGATATT 
ACCACAGGTT GTTGCTCTGG GCAAGTTATG CTAGGTGATA GCAGGGACGT AGTACAAGGT 
GAAAGTTTAC GACTTCCCTG TAGATTTCAT TCAGCCTTAG GATCAAGTAC TACGCTCTAC 
TTTTGGAGTA GAGTGAATAA AGATGGTAAA GACAATGCAG CTATCAATGC TGCGGCATTG 
GACCCTCATT ACAATATAGA CTTCAGCCCT CAAGAGGGCA AATACGATCT GCTCATATCT 
CAAGCGAATT ATGACAAAGA CAATGGAAAG TTCGAATGCT GGTTGAAGGA AGGGGGCAGC 
GGAGTTGACA TCTATAGTGT TTCTTATGTC GTAACTGTTT TAATTGTTCC TGGGGAACCT 
AGAATAACTC CACCAAAGCC CATAGCCAGG GAAGGAGAGT CCACAGCTCT TACTTGCTCA 
AGTGAAGGTG GAAGCCCAGA TCCTACCATC CGCTGGTACA GAGATGGCGT CCTACTTCAA 
GGCATGGTCC AGAAAGGTGG CTCCAGGGAA AGGCCTACGT CCAGCGTACT GACCATCACT 
CCTAGACTAG AAGACGATGA TTCTATCTAT AGATGTGTTG TTTGGAACAG AGCTTTGAGG 
GAGGATCAAA AACTTGAAAC AAGCGTCAGT CTTAGGGTTC ACTATTCACC TAGAATAACT 
GTGGGGCCCT CTAACCCTTT AAACGTCCTA GAAAATACTG ATGCTCATTT AAGCTGCAAT 
GTGATAGCAT CACCCCCTGT TCGTTCTGTC AGGTGGTTGA GGAATGGAAT GCTGATATCT 
AACACTCTCA ATCATACTAT CAGAGGTGTC ACATCTGAAG ACAGTGGTAT TTATAGCTGT 
ATCGCTGAAA ATGGCATTGG AGAACCTACA CAAGCCAACT TGGAATTATC TGTGCTTTAT 
GGCCCCCAAG TGAGACTTCC AACTTATCAG GAAGTAGCTG CTGGCGAACC TCTCATAGTT 
AAATGTGAAG TCTTGTCTAA ACCTCCTCCT CATATGATCC AATGGCTGAA AGAAGGGGAT 
CCGTACTTCA GGCAAACAGG AGACACTCTA CGAATTGATA AAATTACTGT AGATGATGGT 
GGACGATATA TTTGCCAGGC TGCCACTTCA TTCAGACCTT CAGGTTCTAA CATCCAAACT 
GAAGCTACAG GAAATGGAAC AGTTACAGTT CGAATCAAAC ATAAACCTGG CCAGGCTGAA 
ATCCATCCTT CTCAACCTGT AGCCATTGCT GGTCGACCGT TTACACTCTC TTGCACAGCA 
CACCCTCTTG GATGGCCAGT ACCTGAATAC AGGTGGTGGA AAGAAGGCCA GCAGAAAGAA 
GACCTTACTC AGAAACCGAA TTATACCCTA ATATCAGCTC ACATGAGCCA TGAAGGTAGA 
TATTTCTGCC AGCCTTTTAA TAGTCTTGGA AGGGGTGGAT CGGCATCTGT GTATCTAACT 
GTGCATGAAC CAGCATCTAT CCTCATACCA CTTAGACCTC AAACCATCAA AAAGGACAAA 
GAAAGAGATT ACAGTATAAC ATGCAGAGCT CGTGGAAAGC CCAAACCAAA AGTCAGGTGG 
TTACAGAATG GTCAAGAAAT ATCTGAAGAT AGCGGGCTGT TCCATATTTC AAATTCTGAT 
AATGTAGAGG ACAATAATGC ATTCACCGTA CAAAGTACTC TGTCGTTTAC GGGACCCAGT 
CGTCGAGAGA ATGGCCTCAC TCCTGGCGAC AGAGGAAGAT ATACTTGTGA ATTTAATAAT 
GGATTTGGCG AGAATGCTAG GTCTGAAATG ATGCTCAGAA TTGAACATTC CCCTGTGGTC 
CGACATACGT ACAATCGAGT GGCTTTTGAT GTGGGAGAGA CCGCTGTTTT GCAGTGCAAA 
ATGCAGGCTT ACCCTAGCCC AACATTTGAA TGGGTTTCGA GAGGACGTGT TCTGGACGAA 
TATGGGAACT ATGGTACGAA CATCACAGAC CTAGGCGAAG ACATATATGC TGGCGTTCTC 
TCTATTCGAG ACATGAAGGA AGAAGACTAC GGAGAGTACA CGTGTAGAGC TTCCAATCAA 
GTTGGGGATG ACAAGAAAAC CATTATCAGG CTTGTTAGGA AAGGTCCTCC TGAGAGGCCT 
ACTCACTTAG AGTTGATAGA AGTTAGTTCA GATAAAGTGA CTTTGAGATG GCAGGAAGGA 
TTCAATGGTG GTTTCGCCAA CACTGAGTTT TTGGTTACGT ATCTCAATAC TGAAAATGGT 
CGACAAATGA ATGAGTCCTG TCGGACTCAA AATTTGTGCC AAATCACTGG TCTCTCTGCA 
AATACAGAAT ATCACTTCAA AGTTTTGGCT GTTAATCCCC GTGGTTATAG TCCATATTCA 
GAAGAGCTGT CTGTTACAAC TAAAGACATG CCAAGACCAA CAGAAGCACA ATATGATCGT 
GATACTGGCT ATCTTACATT TCATGTTGAA TTAAGTTCAT TAAAATTGTT GGCGAAGATT 
GAGGCTAGAC CTGTGGGATC AGAGATGTGG GTCCTACAGA CTGTGATCTC GGTCATTAGA 
CCCATAGAGA AAGTGCAGTT AAGCCCTCCT GATGAGGGAT TTTCTGATGT GCGTGTCAAA 
CTTTGTCTTG ATAGTAATGA CTCTTGGTGT GGTGATGCCA AATTGGCAAA TCCTTTTGAA 
GAAGAGCTTC CAAATCCCAA AGATTCTAGA GCTGGTTTAC CAACTGAGGC TGTCATGATA 
AGCATTGTTA TTGGAGGTGT TGTATTTTTA TTGGTTATCA TTTTAAGCAT TTGCTGCTGT 
TGGAAGAAGA GAGTAAATCA CAAAGAAAAG AAAGATTTTG AGATGGAAAC AACAAATCCT 
AGACCAAAGG CTGTGACGCC ACCTTACTTC CCAGACGGGG TTGTGAACAA GGGATTGGAG 
AGCGTGACGG ACGACATGAC AAAAGCAGGT ATCTACACCA CCAATACATC TGGCATCAAT 
GGAGGGATCA ATGGCCATAT ACCTTCTGGC AGTCACCTCA ATTCTGGAAT GATGTATAAT 
CCAGATCCTG ATACTAGTCC CTGTAGTGCC CTAGATCATT CAGAACCCTG GTTGAAACCA 
ACAGACGAGC TTGGAACAGA AGGTTCCTAT CAGCCTTATG ATTCTAGGTT ACCTAATGGT 
TATTTTTACC CAGAAGATTA TCAACCTTTA ACAGAAGATT TAATGAACAT TAAAAATAGA 
GACCATATAC ATTCACCATA CTACGATGTG AGTGGTCTGC CGGATCCATA CGCCATGGGC 
GAGGAAGACA AACCACAACA CATCTCCCTC TCGTACGACG AGAGTCATGA AAGTGGCTAC 
TCCACACCTA ACTCTAGATC GAGGAGAGTC ATTCGGGAAA TAATTGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107451685 ( XM_016067870.1 ) cDNA ORF clone, Parasteatoda tepidariorum(common house spider) -> XP_015923356.1 Parasteatoda tepidariorum roundabout homolog 3-like (LOC107451685), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OPa120368
Clone ID Related Accession (Same CDS sequence)	XM_016067870.1 , XM_016067870.2
Accession Version	XM_016067870.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-03-24
Organism	Parasteatoda tepidariorum(Common house spider)
Product	hemicentin-2-like isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015460275.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Parasteatoda tepidariorum Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCAATC GAACTGCAGG CCGGTGCTGT CTTCTGAGTT CTGTTTTATT CTTGGATTTA 
TTCCTTCGAA TATTGTTTTT GTTTGTGGAT GGATGTCGTT TCTTGGATTG GTGTTCTGAA 
GTTAGTGTTT ATTTGCAAGT GCGCGTTCTG TTAGGAATAT TTAAAAACAT GTTGGTGGAC 
CTGTGTACTA TGGATATAAT GATCGCGCGG TTGTGCAATA TTGTGAGGAT TCTAGATATT 
ACCACAGGTT GTTGCTCTGG GCAAGTTATG CTAGGTGATA GCAGGGACGT AGTACAAGGT 
GAAAGTTTAC GACTTCCCTG TAGATTTCAT TCAGCCTTAG GATCAAGTAC TACGCTCTAC 
TTTTGGAGTA GAGTGAATAA AGATGGTAAA GACAATGCAG CTATCAATGC TGCGGCATTG 
GACCCTCATT ACAATATAGA CTTCAGCCCT CAAGAGGGCA AATACGATCT GCTCATATCT 
CAAGCGAATT ATGACAAAGA CAATGGAAAG TTCGAATGCT GGTTGAAGGA AGGGGGCAGC 
GGAGTTGACA TCTATAGTGT TTCTTATGTC GTAACTGTTT TAATTGTTCC TGGGGAACCT 
AGAATAACTC CACCAAAGCC CATAGCCAGG GAAGGAGAGT CCACAGCTCT TACTTGCTCA 
AGTGAAGGTG GAAGCCCAGA TCCTACCATC CGCTGGTACA GAGATGGCGT CCTACTTCAA 
GGCATGGTCC AGAAAGGTGG CTCCAGGGAA AGGCCTACGT CCAGCGTACT GACCATCACT 
CCTAGACTAG AAGACGATGA TTCTATCTAT AGATGTGTTG TTTGGAACAG AGCTTTGAGG 
GAGGATCAAA AACTTGAAAC AAGCGTCAGT CTTAGGGTTC ACTATTCACC TAGAATAACT 
GTGGGGCCCT CTAACCCTTT AAACGTCCTA GAAAATACTG ATGCTCATTT AAGCTGCAAT 
GTGATAGCAT CACCCCCTGT TCGTTCTGTC AGGTGGTTGA GGAATGGAAT GCTGATATCT 
AACACTCTCA ATCATACTAT CAGAGGTGTC ACATCTGAAG ACAGTGGTAT TTATAGCTGT 
ATCGCTGAAA ATGGCATTGG AGAACCTACA CAAGCCAACT TGGAATTATC TGTGCTTTAT 
GGCCCCCAAG TGAGACTTCC AACTTATCAG GAAGTAGCTG CTGGCGAACC TCTCATAGTT 
AAATGTGAAG TCTTGTCTAA ACCTCCTCCT CATATGATCC AATGGCTGAA AGAAGGGGAT 
CCGTACTTCA GGCAAACAGG AGACACTCTA CGAATTGATA AAATTACTGT AGATGATGGT 
GGACGATATA TTTGCCAGGC TGCCACTTCA TTCAGACCTT CAGGTTCTAA CATCCAAACT 
GAAGCTACAG GAAATGGAAC AGTTACAGTT CGAATCAAAC ATAAACCTGG CCAGGCTGAA 
ATCCATCCTT CTCAACCTGT AGCCATTGCT GGTCGACCGT TTACACTCTC TTGCACAGCA 
CACCCTCTTG GATGGCCAGT ACCTGAATAC AGGTGGTGGA AAGAAGGCCA GCAGAAAGAA 
GACCTTACTC AGAAACCGAA TTATACCCTA ATATCAGCTC ACATGAGCCA TGAAGGTAGA 
TATTTCTGCC AGCCTTTTAA TAGTCTTGGA AGGGGTGGAT CGGCATCTGT GTATCTAACT 
GTGCATGAAC CAGCATCTAT CCTCATACCA CTTAGACCTC AAACCATCAA AAAGGACAAA 
GAAAGAGATT ACAGTATAAC ATGCAGAGCT CGTGGAAAGC CCAAACCAAA AGTCAGGTGG 
TTACAGAATG GTCAAGAAAT ATCTGAAGAT AGCGGGCTGT TCCATATTTC AAATTCTGAT 
AATGTAGAGG ACAATAATGC ATTCACCGTA CAAAGTACTC TGTCGTTTAC GGGACCCAGT 
CGTCGAGAGA ATGGCCTCAC TCCTGGCGAC AGAGGAAGAT ATACTTGTGA ATTTAATAAT 
GGATTTGGCG AGAATGCTAG GTCTGAAATG ATGCTCAGAA TTGAACATTC CCCTGTGGTC 
CGACATACGT ACAATCGAGT GGCTTTTGAT GTGGGAGAGA CCGCTGTTTT GCAGTGCAAA 
ATGCAGGCTT ACCCTAGCCC AACATTTGAA TGGGTTTCGA GAGGACGTGT TCTGGACGAA 
TATGGGAACT ATGGTACGAA CATCACAGAC CTAGGCGAAG ACATATATGC TGGCGTTCTC 
TCTATTCGAG ACATGAAGGA AGAAGACTAC GGAGAGTACA CGTGTAGAGC TTCCAATCAA 
GTTGGGGATG ACAAGAAAAC CATTATCAGG CTTGTTAGGA AAGGTCCTCC TGAGAGGCCT 
ACTCACTTAG AGTTGATAGA AGTTAGTTCA GATAAAGTGA CTTTGAGATG GCAGGAAGGA 
TTCAATGGTG GTTTCGCCAA CACTGAGTTT TTGGTTACGT ATCTCAATAC TGAAAATGGT 
CGACAAATGA ATGAGTCCTG TCGGACTCAA AATTTGTGCC AAATCACTGG TCTCTCTGCA 
AATACAGAAT ATCACTTCAA AGTTTTGGCT GTTAATCCCC GTGGTTATAG TCCATATTCA 
GAAGAGCTGT CTGTTACAAC TAAAGTGAAT TTAAGAGACA TGCCAAGACC AACAGAAGCA 
CAATATGATC GTGATACTGG CTATCTTACA TTTCATGTTG AATTAAGTTC ATTAAAATTG 
TTGGCGAAGA TTGAGGCTAG ACCTGTGGGA TCAGAGATGT GGGTCCTACA GACTGTGATC 
TCGGTCATTA GACCCATAGA GAAAGTGCAG TTAAGCCCTC CTGATGAGGG ATTTTCTGAT 
GTGCGTGTCA AACTTTGTCT TGATAGTAAT GACTCTTGGT GTGGTGATGC CAAATTGGCA 
AATCCTTTTG AAGAAGAGCT TCCAAATCCC AAAGATTCTA GAGCTGGTTT ACCAACTGAG 
GCTGTCATGA TAAGCATTGT TATTGGAGGT GTTGTATTTT TATTGGTTAT CATTTTAAGC 
ATTTGCTGCT GTTGGAAGAA GAGAGTAAAT CACAAAGAAA AGAAAGATTT TGAGATGGAA 
ACAACAAATC CTAGACCAAA GGCTGTGACG CCACCTTACT TCCCAGACGG GGTTGTGAAC 
AAGGGATTGG AGAGCGTGAC GGACGACATG ACAAAAGCAG GAATGATGTA TAATCCAGAT 
CCTGATACTA GTCCCTGTAG TGCCCTAGAT CATTCAGAAC CCTGGTTGAA ACCAACAGAC 
GAGCTTGGAA CAGAAGGTTC CTATCAGCCT TATGATTCTA GGTTACCTAA TGGTTATTTT 
TACCCAGAAG ATTATCAACC TTTAACAGAA GATTTAATGA ACATTAAAAA TAGAGACCAT 
ATACATTCAC CATACTACGA TGTGAGTGGT CTGCCGGATC CATACGCCAT GGGCGAGGAA 
GACAAACCAC AACACATCTC CCTCTCGTAC GACGAGAGTC ATGAAAGTGG CTACTCCACA 
CCTAACTCTA GATCGAGGAG AGTCATTCGG GAAATAATTG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015923356.1
CDS	121..3645
Translation	MTNRTAGRCCLLSSVLFLDLFLRILFLFVDGCRFLDWCSEVSVYLQVRVLLGIFKNMLVDLCTMDIMIARLCNIVRILDITTGCCSGQVMLGDSRDVVQGESLRLPCRFHSALGSSTTLYFWSRVNKDGKDNAAINAAALDPHYNIDFSPQEGKYDLLISQANYDKDNGKFECWLKEGGSGVDIYSVSYVVTVLIVPGEPRITPPKPIAREGESTALTCSSEGGSPDPTIRWYRDGVLLQGMVQKGGSRERPTSSVLTITPRLEDDDSIYRCVVWNRALREDQKLETSVSLRVHYSPRITVGPSNPLNVLENTDAHLSCNVIASPPVRSVRWLRNGMLISNTLNHTIRGVTSEDSGIYSCIAENGIGEPTQANLELSVLYGPQVRLPTYQEVAAGEPLIVKCEVLSKPPPHMIQWLKEGDPYFRQTGDTLRIDKITVDDGGRYICQAATSFRPSGSNIQTEATGNGTVTVRIKHKPGQAEIHPSQPVAIAGRPFTLSCTAHPLGWPVPEYRWWKEGQQKEDLTQKPNYTLISAHMSHEGRYFCQPFNSLGRGGSASVYLTVHEPASILIPLRPQTIKKDKERDYSITCRARGKPKPKVRWLQNGQEISEDSGLFHISNSDNVEDNNAFTVQSTLSFTGPSRRENGLTPGDRGRYTCEFNNGFGENARSEMMLRIEHSPVVRHTYNRVAFDVGETAVLQCKMQAYPSPTFEWVSRGRVLDEYGNYGTNITDLGEDIYAGVLSIRDMKEEDYGEYTCRASNQVGDDKKTIIRLVRKGPPERPTHLELIEVSSDKVTLRWQEGFNGGFANTEFLVTYLNTENGRQMNESCRTQNLCQITGLSANTEYHFKVLAVNPRGYSPYSEELSVTTKVNLRDMPRPTEAQYDRDTGYLTFHVELSSLKLLAKIEARPVGSEMWVLQTVISVIRPIEKVQLSPPDEGFSDVRVKLCLDSNDSWCGDAKLANPFEEELPNPKDSRAGLPTEAVMISIVIGGVVFLLVIILSICCCWKKRVNHKEKKDFEMETTNPRPKAVTPPYFPDGVVNKGLESVTDDMTKAGMMYNPDPDTSPCSALDHSEPWLKPTDELGTEGSYQPYDSRLPNGYFYPEDYQPLTEDLMNIKNRDHIHSPYYDVSGLPDPYAMGEEDKPQHISLSYDESHESGYSTPNSRSRRVIREIIV

Target ORF information:

RefSeq Version	XM_016067870.1
Organism	Parasteatoda tepidariorum(Common house spider)
Definition	Parasteatoda tepidariorum roundabout homolog 3-like (LOC107451685), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016067870.1

ORF Insert Sequence:

ATGACCAATC GAACTGCAGG CCGGTGCTGT CTTCTGAGTT CTGTTTTATT CTTGGATTTA 
TTCCTTCGAA TATTGTTTTT GTTTGTGGAT GGATGTCGTT TCTTGGATTG GTGTTCTGAA 
GTTAGTGTTT ATTTGCAAGT GCGCGTTCTG TTAGGAATAT TTAAAAACAT GTTGGTGGAC 
CTGTGTACTA TGGATATAAT GATCGCGCGG TTGTGCAATA TTGTGAGGAT TCTAGATATT 
ACCACAGGTT GTTGCTCTGG GCAAGTTATG CTAGGTGATA GCAGGGACGT AGTACAAGGT 
GAAAGTTTAC GACTTCCCTG TAGATTTCAT TCAGCCTTAG GATCAAGTAC TACGCTCTAC 
TTTTGGAGTA GAGTGAATAA AGATGGTAAA GACAATGCAG CTATCAATGC TGCGGCATTG 
GACCCTCATT ACAATATAGA CTTCAGCCCT CAAGAGGGCA AATACGATCT GCTCATATCT 
CAAGCGAATT ATGACAAAGA CAATGGAAAG TTCGAATGCT GGTTGAAGGA AGGGGGCAGC 
GGAGTTGACA TCTATAGTGT TTCTTATGTC GTAACTGTTT TAATTGTTCC TGGGGAACCT 
AGAATAACTC CACCAAAGCC CATAGCCAGG GAAGGAGAGT CCACAGCTCT TACTTGCTCA 
AGTGAAGGTG GAAGCCCAGA TCCTACCATC CGCTGGTACA GAGATGGCGT CCTACTTCAA 
GGCATGGTCC AGAAAGGTGG CTCCAGGGAA AGGCCTACGT CCAGCGTACT GACCATCACT 
CCTAGACTAG AAGACGATGA TTCTATCTAT AGATGTGTTG TTTGGAACAG AGCTTTGAGG 
GAGGATCAAA AACTTGAAAC AAGCGTCAGT CTTAGGGTTC ACTATTCACC TAGAATAACT 
GTGGGGCCCT CTAACCCTTT AAACGTCCTA GAAAATACTG ATGCTCATTT AAGCTGCAAT 
GTGATAGCAT CACCCCCTGT TCGTTCTGTC AGGTGGTTGA GGAATGGAAT GCTGATATCT 
AACACTCTCA ATCATACTAT CAGAGGTGTC ACATCTGAAG ACAGTGGTAT TTATAGCTGT 
ATCGCTGAAA ATGGCATTGG AGAACCTACA CAAGCCAACT TGGAATTATC TGTGCTTTAT 
GGCCCCCAAG TGAGACTTCC AACTTATCAG GAAGTAGCTG CTGGCGAACC TCTCATAGTT 
AAATGTGAAG TCTTGTCTAA ACCTCCTCCT CATATGATCC AATGGCTGAA AGAAGGGGAT 
CCGTACTTCA GGCAAACAGG AGACACTCTA CGAATTGATA AAATTACTGT AGATGATGGT 
GGACGATATA TTTGCCAGGC TGCCACTTCA TTCAGACCTT CAGGTTCTAA CATCCAAACT 
GAAGCTACAG GAAATGGAAC AGTTACAGTT CGAATCAAAC ATAAACCTGG CCAGGCTGAA 
ATCCATCCTT CTCAACCTGT AGCCATTGCT GGTCGACCGT TTACACTCTC TTGCACAGCA 
CACCCTCTTG GATGGCCAGT ACCTGAATAC AGGTGGTGGA AAGAAGGCCA GCAGAAAGAA 
GACCTTACTC AGAAACCGAA TTATACCCTA ATATCAGCTC ACATGAGCCA TGAAGGTAGA 
TATTTCTGCC AGCCTTTTAA TAGTCTTGGA AGGGGTGGAT CGGCATCTGT GTATCTAACT 
GTGCATGAAC CAGCATCTAT CCTCATACCA CTTAGACCTC AAACCATCAA AAAGGACAAA 
GAAAGAGATT ACAGTATAAC ATGCAGAGCT CGTGGAAAGC CCAAACCAAA AGTCAGGTGG 
TTACAGAATG GTCAAGAAAT ATCTGAAGAT AGCGGGCTGT TCCATATTTC AAATTCTGAT 
AATGTAGAGG ACAATAATGC ATTCACCGTA CAAAGTACTC TGTCGTTTAC GGGACCCAGT 
CGTCGAGAGA ATGGCCTCAC TCCTGGCGAC AGAGGAAGAT ATACTTGTGA ATTTAATAAT 
GGATTTGGCG AGAATGCTAG GTCTGAAATG ATGCTCAGAA TTGAACATTC CCCTGTGGTC 
CGACATACGT ACAATCGAGT GGCTTTTGAT GTGGGAGAGA CCGCTGTTTT GCAGTGCAAA 
ATGCAGGCTT ACCCTAGCCC AACATTTGAA TGGGTTTCGA GAGGACGTGT TCTGGACGAA 
TATGGGAACT ATGGTACGAA CATCACAGAC CTAGGCGAAG ACATATATGC TGGCGTTCTC 
TCTATTCGAG ACATGAAGGA AGAAGACTAC GGAGAGTACA CGTGTAGAGC TTCCAATCAA 
GTTGGGGATG ACAAGAAAAC CATTATCAGG CTTGTTAGGA AAGGTCCTCC TGAGAGGCCT 
ACTCACTTAG AGTTGATAGA AGTTAGTTCA GATAAAGTGA CTTTGAGATG GCAGGAAGGA 
TTCAATGGTG GTTTCGCCAA CACTGAGTTT TTGGTTACGT ATCTCAATAC TGAAAATGGT 
CGACAAATGA ATGAGTCCTG TCGGACTCAA AATTTGTGCC AAATCACTGG TCTCTCTGCA 
AATACAGAAT ATCACTTCAA AGTTTTGGCT GTTAATCCCC GTGGTTATAG TCCATATTCA 
GAAGAGCTGT CTGTTACAAC TAAAGTGAAT TTAAGAGACA TGCCAAGACC AACAGAAGCA 
CAATATGATC GTGATACTGG CTATCTTACA TTTCATGTTG AATTAAGTTC ATTAAAATTG 
TTGGCGAAGA TTGAGGCTAG ACCTGTGGGA TCAGAGATGT GGGTCCTACA GACTGTGATC 
TCGGTCATTA GACCCATAGA GAAAGTGCAG TTAAGCCCTC CTGATGAGGG ATTTTCTGAT 
GTGCGTGTCA AACTTTGTCT TGATAGTAAT GACTCTTGGT GTGGTGATGC CAAATTGGCA 
AATCCTTTTG AAGAAGAGCT TCCAAATCCC AAAGATTCTA GAGCTGGTTT ACCAACTGAG 
GCTGTCATGA TAAGCATTGT TATTGGAGGT GTTGTATTTT TATTGGTTAT CATTTTAAGC 
ATTTGCTGCT GTTGGAAGAA GAGAGTAAAT CACAAAGAAA AGAAAGATTT TGAGATGGAA 
ACAACAAATC CTAGACCAAA GGCTGTGACG CCACCTTACT TCCCAGACGG GGTTGTGAAC 
AAGGGATTGG AGAGCGTGAC GGACGACATG ACAAAAGCAG GAATGATGTA TAATCCAGAT 
CCTGATACTA GTCCCTGTAG TGCCCTAGAT CATTCAGAAC CCTGGTTGAA ACCAACAGAC 
GAGCTTGGAA CAGAAGGTTC CTATCAGCCT TATGATTCTA GGTTACCTAA TGGTTATTTT 
TACCCAGAAG ATTATCAACC TTTAACAGAA GATTTAATGA ACATTAAAAA TAGAGACCAT 
ATACATTCAC CATACTACGA TGTGAGTGGT CTGCCGGATC CATACGCCAT GGGCGAGGAA 
GACAAACCAC AACACATCTC CCTCTCGTAC GACGAGAGTC ATGAAAGTGG CTACTCCACA 
CCTAACTCTA GATCGAGGAG AGTCATTCGG GAAATAATTG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107451685 ( XM_016067870.2 ) cDNA ORF clone, Parasteatoda tepidariorum(common house spider) -> XP_015923356.1 Parasteatoda tepidariorum roundabout homolog 3 (LOC107451685), transcript variant X3, mRNA.

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CloneID	OPa120368
Clone ID Related Accession (Same CDS sequence)	XM_016067870.1 , XM_016067870.2
Accession Version	XM_016067870.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-14
Organism	Parasteatoda tepidariorum(common house spider)
Product	hemicentin-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018372425.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 15, 2017 this sequence version replaced XM_016067870.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Parasteatoda tepidariorum Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCAATC GAACTGCAGG CCGGTGCTGT CTTCTGAGTT CTGTTTTATT CTTGGATTTA 
TTCCTTCGAA TATTGTTTTT GTTTGTGGAT GGATGTCGTT TCTTGGATTG GTGTTCTGAA 
GTTAGTGTTT ATTTGCAAGT GCGCGTTCTG TTAGGAATAT TTAAAAACAT GTTGGTGGAC 
CTGTGTACTA TGGATATAAT GATCGCGCGG TTGTGCAATA TTGTGAGGAT TCTAGATATT 
ACCACAGGTT GTTGCTCTGG GCAAGTTATG CTAGGTGATA GCAGGGACGT AGTACAAGGT 
GAAAGTTTAC GACTTCCCTG TAGATTTCAT TCAGCCTTAG GATCAAGTAC TACGCTCTAC 
TTTTGGAGTA GAGTGAATAA AGATGGTAAA GACAATGCAG CTATCAATGC TGCGGCATTG 
GACCCTCATT ACAATATAGA CTTCAGCCCT CAAGAGGGCA AATACGATCT GCTCATATCT 
CAAGCGAATT ATGACAAAGA CAATGGAAAG TTCGAATGCT GGTTGAAGGA AGGGGGCAGC 
GGAGTTGACA TCTATAGTGT TTCTTATGTC GTAACTGTTT TAATTGTTCC TGGGGAACCT 
AGAATAACTC CACCAAAGCC CATAGCCAGG GAAGGAGAGT CCACAGCTCT TACTTGCTCA 
AGTGAAGGTG GAAGCCCAGA TCCTACCATC CGCTGGTACA GAGATGGCGT CCTACTTCAA 
GGCATGGTCC AGAAAGGTGG CTCCAGGGAA AGGCCTACGT CCAGCGTACT GACCATCACT 
CCTAGACTAG AAGACGATGA TTCTATCTAT AGATGTGTTG TTTGGAACAG AGCTTTGAGG 
GAGGATCAAA AACTTGAAAC AAGCGTCAGT CTTAGGGTTC ACTATTCACC TAGAATAACT 
GTGGGGCCCT CTAACCCTTT AAACGTCCTA GAAAATACTG ATGCTCATTT AAGCTGCAAT 
GTGATAGCAT CACCCCCTGT TCGTTCTGTC AGGTGGTTGA GGAATGGAAT GCTGATATCT 
AACACTCTCA ATCATACTAT CAGAGGTGTC ACATCTGAAG ACAGTGGTAT TTATAGCTGT 
ATCGCTGAAA ATGGCATTGG AGAACCTACA CAAGCCAACT TGGAATTATC TGTGCTTTAT 
GGCCCCCAAG TGAGACTTCC AACTTATCAG GAAGTAGCTG CTGGCGAACC TCTCATAGTT 
AAATGTGAAG TCTTGTCTAA ACCTCCTCCT CATATGATCC AATGGCTGAA AGAAGGGGAT 
CCGTACTTCA GGCAAACAGG AGACACTCTA CGAATTGATA AAATTACTGT AGATGATGGT 
GGACGATATA TTTGCCAGGC TGCCACTTCA TTCAGACCTT CAGGTTCTAA CATCCAAACT 
GAAGCTACAG GAAATGGAAC AGTTACAGTT CGAATCAAAC ATAAACCTGG CCAGGCTGAA 
ATCCATCCTT CTCAACCTGT AGCCATTGCT GGTCGACCGT TTACACTCTC TTGCACAGCA 
CACCCTCTTG GATGGCCAGT ACCTGAATAC AGGTGGTGGA AAGAAGGCCA GCAGAAAGAA 
GACCTTACTC AGAAACCGAA TTATACCCTA ATATCAGCTC ACATGAGCCA TGAAGGTAGA 
TATTTCTGCC AGCCTTTTAA TAGTCTTGGA AGGGGTGGAT CGGCATCTGT GTATCTAACT 
GTGCATGAAC CAGCATCTAT CCTCATACCA CTTAGACCTC AAACCATCAA AAAGGACAAA 
GAAAGAGATT ACAGTATAAC ATGCAGAGCT CGTGGAAAGC CCAAACCAAA AGTCAGGTGG 
TTACAGAATG GTCAAGAAAT ATCTGAAGAT AGCGGGCTGT TCCATATTTC AAATTCTGAT 
AATGTAGAGG ACAATAATGC ATTCACCGTA CAAAGTACTC TGTCGTTTAC GGGACCCAGT 
CGTCGAGAGA ATGGCCTCAC TCCTGGCGAC AGAGGAAGAT ATACTTGTGA ATTTAATAAT 
GGATTTGGCG AGAATGCTAG GTCTGAAATG ATGCTCAGAA TTGAACATTC CCCTGTGGTC 
CGACATACGT ACAATCGAGT GGCTTTTGAT GTGGGAGAGA CCGCTGTTTT GCAGTGCAAA 
ATGCAGGCTT ACCCTAGCCC AACATTTGAA TGGGTTTCGA GAGGACGTGT TCTGGACGAA 
TATGGGAACT ATGGTACGAA CATCACAGAC CTAGGCGAAG ACATATATGC TGGCGTTCTC 
TCTATTCGAG ACATGAAGGA AGAAGACTAC GGAGAGTACA CGTGTAGAGC TTCCAATCAA 
GTTGGGGATG ACAAGAAAAC CATTATCAGG CTTGTTAGGA AAGGTCCTCC TGAGAGGCCT 
ACTCACTTAG AGTTGATAGA AGTTAGTTCA GATAAAGTGA CTTTGAGATG GCAGGAAGGA 
TTCAATGGTG GTTTCGCCAA CACTGAGTTT TTGGTTACGT ATCTCAATAC TGAAAATGGT 
CGACAAATGA ATGAGTCCTG TCGGACTCAA AATTTGTGCC AAATCACTGG TCTCTCTGCA 
AATACAGAAT ATCACTTCAA AGTTTTGGCT GTTAATCCCC GTGGTTATAG TCCATATTCA 
GAAGAGCTGT CTGTTACAAC TAAAGTGAAT TTAAGAGACA TGCCAAGACC AACAGAAGCA 
CAATATGATC GTGATACTGG CTATCTTACA TTTCATGTTG AATTAAGTTC ATTAAAATTG 
TTGGCGAAGA TTGAGGCTAG ACCTGTGGGA TCAGAGATGT GGGTCCTACA GACTGTGATC 
TCGGTCATTA GACCCATAGA GAAAGTGCAG TTAAGCCCTC CTGATGAGGG ATTTTCTGAT 
GTGCGTGTCA AACTTTGTCT TGATAGTAAT GACTCTTGGT GTGGTGATGC CAAATTGGCA 
AATCCTTTTG AAGAAGAGCT TCCAAATCCC AAAGATTCTA GAGCTGGTTT ACCAACTGAG 
GCTGTCATGA TAAGCATTGT TATTGGAGGT GTTGTATTTT TATTGGTTAT CATTTTAAGC 
ATTTGCTGCT GTTGGAAGAA GAGAGTAAAT CACAAAGAAA AGAAAGATTT TGAGATGGAA 
ACAACAAATC CTAGACCAAA GGCTGTGACG CCACCTTACT TCCCAGACGG GGTTGTGAAC 
AAGGGATTGG AGAGCGTGAC GGACGACATG ACAAAAGCAG GAATGATGTA TAATCCAGAT 
CCTGATACTA GTCCCTGTAG TGCCCTAGAT CATTCAGAAC CCTGGTTGAA ACCAACAGAC 
GAGCTTGGAA CAGAAGGTTC CTATCAGCCT TATGATTCTA GGTTACCTAA TGGTTATTTT 
TACCCAGAAG ATTATCAACC TTTAACAGAA GATTTAATGA ACATTAAAAA TAGAGACCAT 
ATACATTCAC CATACTACGA TGTGAGTGGT CTGCCGGATC CATACGCCAT GGGCGAGGAA 
GACAAACCAC AACACATCTC CCTCTCGTAC GACGAGAGTC ATGAAAGTGG CTACTCCACA 
CCTAACTCTA GATCGAGGAG AGTCATTCGG GAAATAATTG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015923356.1
CDS	122..3646
Translation	MTNRTAGRCCLLSSVLFLDLFLRILFLFVDGCRFLDWCSEVSVYLQVRVLLGIFKNMLVDLCTMDIMIARLCNIVRILDITTGCCSGQVMLGDSRDVVQGESLRLPCRFHSALGSSTTLYFWSRVNKDGKDNAAINAAALDPHYNIDFSPQEGKYDLLISQANYDKDNGKFECWLKEGGSGVDIYSVSYVVTVLIVPGEPRITPPKPIAREGESTALTCSSEGGSPDPTIRWYRDGVLLQGMVQKGGSRERPTSSVLTITPRLEDDDSIYRCVVWNRALREDQKLETSVSLRVHYSPRITVGPSNPLNVLENTDAHLSCNVIASPPVRSVRWLRNGMLISNTLNHTIRGVTSEDSGIYSCIAENGIGEPTQANLELSVLYGPQVRLPTYQEVAAGEPLIVKCEVLSKPPPHMIQWLKEGDPYFRQTGDTLRIDKITVDDGGRYICQAATSFRPSGSNIQTEATGNGTVTVRIKHKPGQAEIHPSQPVAIAGRPFTLSCTAHPLGWPVPEYRWWKEGQQKEDLTQKPNYTLISAHMSHEGRYFCQPFNSLGRGGSASVYLTVHEPASILIPLRPQTIKKDKERDYSITCRARGKPKPKVRWLQNGQEISEDSGLFHISNSDNVEDNNAFTVQSTLSFTGPSRRENGLTPGDRGRYTCEFNNGFGENARSEMMLRIEHSPVVRHTYNRVAFDVGETAVLQCKMQAYPSPTFEWVSRGRVLDEYGNYGTNITDLGEDIYAGVLSIRDMKEEDYGEYTCRASNQVGDDKKTIIRLVRKGPPERPTHLELIEVSSDKVTLRWQEGFNGGFANTEFLVTYLNTENGRQMNESCRTQNLCQITGLSANTEYHFKVLAVNPRGYSPYSEELSVTTKVNLRDMPRPTEAQYDRDTGYLTFHVELSSLKLLAKIEARPVGSEMWVLQTVISVIRPIEKVQLSPPDEGFSDVRVKLCLDSNDSWCGDAKLANPFEEELPNPKDSRAGLPTEAVMISIVIGGVVFLLVIILSICCCWKKRVNHKEKKDFEMETTNPRPKAVTPPYFPDGVVNKGLESVTDDMTKAGMMYNPDPDTSPCSALDHSEPWLKPTDELGTEGSYQPYDSRLPNGYFYPEDYQPLTEDLMNIKNRDHIHSPYYDVSGLPDPYAMGEEDKPQHISLSYDESHESGYSTPNSRSRRVIREIIV

Target ORF information:

RefSeq Version	XM_016067870.2
Organism	Parasteatoda tepidariorum(common house spider)
Definition	Parasteatoda tepidariorum roundabout homolog 3 (LOC107451685), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016067870.2

ORF Insert Sequence:

ATGACCAATC GAACTGCAGG CCGGTGCTGT CTTCTGAGTT CTGTTTTATT CTTGGATTTA 
TTCCTTCGAA TATTGTTTTT GTTTGTGGAT GGATGTCGTT TCTTGGATTG GTGTTCTGAA 
GTTAGTGTTT ATTTGCAAGT GCGCGTTCTG TTAGGAATAT TTAAAAACAT GTTGGTGGAC 
CTGTGTACTA TGGATATAAT GATCGCGCGG TTGTGCAATA TTGTGAGGAT TCTAGATATT 
ACCACAGGTT GTTGCTCTGG GCAAGTTATG CTAGGTGATA GCAGGGACGT AGTACAAGGT 
GAAAGTTTAC GACTTCCCTG TAGATTTCAT TCAGCCTTAG GATCAAGTAC TACGCTCTAC 
TTTTGGAGTA GAGTGAATAA AGATGGTAAA GACAATGCAG CTATCAATGC TGCGGCATTG 
GACCCTCATT ACAATATAGA CTTCAGCCCT CAAGAGGGCA AATACGATCT GCTCATATCT 
CAAGCGAATT ATGACAAAGA CAATGGAAAG TTCGAATGCT GGTTGAAGGA AGGGGGCAGC 
GGAGTTGACA TCTATAGTGT TTCTTATGTC GTAACTGTTT TAATTGTTCC TGGGGAACCT 
AGAATAACTC CACCAAAGCC CATAGCCAGG GAAGGAGAGT CCACAGCTCT TACTTGCTCA 
AGTGAAGGTG GAAGCCCAGA TCCTACCATC CGCTGGTACA GAGATGGCGT CCTACTTCAA 
GGCATGGTCC AGAAAGGTGG CTCCAGGGAA AGGCCTACGT CCAGCGTACT GACCATCACT 
CCTAGACTAG AAGACGATGA TTCTATCTAT AGATGTGTTG TTTGGAACAG AGCTTTGAGG 
GAGGATCAAA AACTTGAAAC AAGCGTCAGT CTTAGGGTTC ACTATTCACC TAGAATAACT 
GTGGGGCCCT CTAACCCTTT AAACGTCCTA GAAAATACTG ATGCTCATTT AAGCTGCAAT 
GTGATAGCAT CACCCCCTGT TCGTTCTGTC AGGTGGTTGA GGAATGGAAT GCTGATATCT 
AACACTCTCA ATCATACTAT CAGAGGTGTC ACATCTGAAG ACAGTGGTAT TTATAGCTGT 
ATCGCTGAAA ATGGCATTGG AGAACCTACA CAAGCCAACT TGGAATTATC TGTGCTTTAT 
GGCCCCCAAG TGAGACTTCC AACTTATCAG GAAGTAGCTG CTGGCGAACC TCTCATAGTT 
AAATGTGAAG TCTTGTCTAA ACCTCCTCCT CATATGATCC AATGGCTGAA AGAAGGGGAT 
CCGTACTTCA GGCAAACAGG AGACACTCTA CGAATTGATA AAATTACTGT AGATGATGGT 
GGACGATATA TTTGCCAGGC TGCCACTTCA TTCAGACCTT CAGGTTCTAA CATCCAAACT 
GAAGCTACAG GAAATGGAAC AGTTACAGTT CGAATCAAAC ATAAACCTGG CCAGGCTGAA 
ATCCATCCTT CTCAACCTGT AGCCATTGCT GGTCGACCGT TTACACTCTC TTGCACAGCA 
CACCCTCTTG GATGGCCAGT ACCTGAATAC AGGTGGTGGA AAGAAGGCCA GCAGAAAGAA 
GACCTTACTC AGAAACCGAA TTATACCCTA ATATCAGCTC ACATGAGCCA TGAAGGTAGA 
TATTTCTGCC AGCCTTTTAA TAGTCTTGGA AGGGGTGGAT CGGCATCTGT GTATCTAACT 
GTGCATGAAC CAGCATCTAT CCTCATACCA CTTAGACCTC AAACCATCAA AAAGGACAAA 
GAAAGAGATT ACAGTATAAC ATGCAGAGCT CGTGGAAAGC CCAAACCAAA AGTCAGGTGG 
TTACAGAATG GTCAAGAAAT ATCTGAAGAT AGCGGGCTGT TCCATATTTC AAATTCTGAT 
AATGTAGAGG ACAATAATGC ATTCACCGTA CAAAGTACTC TGTCGTTTAC GGGACCCAGT 
CGTCGAGAGA ATGGCCTCAC TCCTGGCGAC AGAGGAAGAT ATACTTGTGA ATTTAATAAT 
GGATTTGGCG AGAATGCTAG GTCTGAAATG ATGCTCAGAA TTGAACATTC CCCTGTGGTC 
CGACATACGT ACAATCGAGT GGCTTTTGAT GTGGGAGAGA CCGCTGTTTT GCAGTGCAAA 
ATGCAGGCTT ACCCTAGCCC AACATTTGAA TGGGTTTCGA GAGGACGTGT TCTGGACGAA 
TATGGGAACT ATGGTACGAA CATCACAGAC CTAGGCGAAG ACATATATGC TGGCGTTCTC 
TCTATTCGAG ACATGAAGGA AGAAGACTAC GGAGAGTACA CGTGTAGAGC TTCCAATCAA 
GTTGGGGATG ACAAGAAAAC CATTATCAGG CTTGTTAGGA AAGGTCCTCC TGAGAGGCCT 
ACTCACTTAG AGTTGATAGA AGTTAGTTCA GATAAAGTGA CTTTGAGATG GCAGGAAGGA 
TTCAATGGTG GTTTCGCCAA CACTGAGTTT TTGGTTACGT ATCTCAATAC TGAAAATGGT 
CGACAAATGA ATGAGTCCTG TCGGACTCAA AATTTGTGCC AAATCACTGG TCTCTCTGCA 
AATACAGAAT ATCACTTCAA AGTTTTGGCT GTTAATCCCC GTGGTTATAG TCCATATTCA 
GAAGAGCTGT CTGTTACAAC TAAAGTGAAT TTAAGAGACA TGCCAAGACC AACAGAAGCA 
CAATATGATC GTGATACTGG CTATCTTACA TTTCATGTTG AATTAAGTTC ATTAAAATTG 
TTGGCGAAGA TTGAGGCTAG ACCTGTGGGA TCAGAGATGT GGGTCCTACA GACTGTGATC 
TCGGTCATTA GACCCATAGA GAAAGTGCAG TTAAGCCCTC CTGATGAGGG ATTTTCTGAT 
GTGCGTGTCA AACTTTGTCT TGATAGTAAT GACTCTTGGT GTGGTGATGC CAAATTGGCA 
AATCCTTTTG AAGAAGAGCT TCCAAATCCC AAAGATTCTA GAGCTGGTTT ACCAACTGAG 
GCTGTCATGA TAAGCATTGT TATTGGAGGT GTTGTATTTT TATTGGTTAT CATTTTAAGC 
ATTTGCTGCT GTTGGAAGAA GAGAGTAAAT CACAAAGAAA AGAAAGATTT TGAGATGGAA 
ACAACAAATC CTAGACCAAA GGCTGTGACG CCACCTTACT TCCCAGACGG GGTTGTGAAC 
AAGGGATTGG AGAGCGTGAC GGACGACATG ACAAAAGCAG GAATGATGTA TAATCCAGAT 
CCTGATACTA GTCCCTGTAG TGCCCTAGAT CATTCAGAAC CCTGGTTGAA ACCAACAGAC 
GAGCTTGGAA CAGAAGGTTC CTATCAGCCT TATGATTCTA GGTTACCTAA TGGTTATTTT 
TACCCAGAAG ATTATCAACC TTTAACAGAA GATTTAATGA ACATTAAAAA TAGAGACCAT 
ATACATTCAC CATACTACGA TGTGAGTGGT CTGCCGGATC CATACGCCAT GGGCGAGGAA 
GACAAACCAC AACACATCTC CCTCTCGTAC GACGAGAGTC ATGAAAGTGG CTACTCCACA 
CCTAACTCTA GATCGAGGAG AGTCATTCGG GAAATAATTG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107451685 ( XM_016067877.1 ) cDNA ORF clone, Parasteatoda tepidariorum(common house spider) -> XP_015923363.1 Parasteatoda tepidariorum roundabout homolog 3-like (LOC107451685), transcript variant X4, mRNA.

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CloneID	OPa120369
Clone ID Related Accession (Same CDS sequence)	XM_016067877.1 , XM_016067877.2
Accession Version	XM_016067877.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3516bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-03-24
Organism	Parasteatoda tepidariorum(Common house spider)
Product	hemicentin-2-like isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015460275.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Parasteatoda tepidariorum Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGAGTG CAAAACGGAC CAATTCCTGG ATTACAATGT TTAGGTGTTC ATCTAGTACT 
CTGTGCGTGT GCCTTATGTT TATATTATTG TTCCTGTGTT TGTTGTCAAC AGGGGGCGTA 
AAAGCTGCTC CTCAGTACTT CACCGATTTG GATGCAGAAG GTTGTTGCTC TGGGCAAGTT 
ATGCTAGGTG ATAGCAGGGA CGTAGTACAA GGTGAAAGTT TACGACTTCC CTGTAGATTT 
CATTCAGCCT TAGGATCAAG TACTACGCTC TACTTTTGGA GTAGAGTGAA TAAAGATGGT 
AAAGACAATG CAGCTATCAA TGCTGCGGCA TTGGACCCTC ATTACAATAT AGACTTCAGC 
CCTCAAGAGG GCAAATACGA TCTGCTCATA TCTCAAGCGA ATTATGACAA AGACAATGGA 
AAGTTCGAAT GCTGGTTGAA GGAAGGGGGC AGCGGAGTTG ACATCTATAG TGTTTCTTAT 
GTCGTAACTG TTTTAATTGT TCCTGGGGAA CCTAGAATAA CTCCACCAAA GCCCATAGCC 
AGGGAAGGAG AGTCCACAGC TCTTACTTGC TCAAGTGAAG GTGGAAGCCC AGATCCTACC 
ATCCGCTGGT ACAGAGATGG CGTCCTACTT CAAGGCATGG TCCAGAAAGG TGGCTCCAGG 
GAAAGGCCTA CGTCCAGCGT ACTGACCATC ACTCCTAGAC TAGAAGACGA TGATTCTATC 
TATAGATGTG TTGTTTGGAA CAGAGCTTTG AGGGAGGATC AAAAACTTGA AACAAGCGTC 
AGTCTTAGGG TTCACTATTC ACCTAGAATA ACTGTGGGGC CCTCTAACCC TTTAAACGTC 
CTAGAAAATA CTGATGCTCA TTTAAGCTGC AATGTGATAG CATCACCCCC TGTTCGTTCT 
GTCAGGTGGT TGAGGAATGG AATGCTGATA TCTAACACTC TCAATCATAC TATCAGAGGT 
GTCACATCTG AAGACAGTGG TATTTATAGC TGTATCGCTG AAAATGGCAT TGGAGAACCT 
ACACAAGCCA ACTTGGAATT ATCTGTGCTT TATGGCCCCC AAGTGAGACT TCCAACTTAT 
CAGGAAGTAG CTGCTGGCGA ACCTCTCATA GTTAAATGTG AAGTCTTGTC TAAACCTCCT 
CCTCATATGA TCCAATGGCT GAAAGAAGGG GATCCGTACT TCAGGCAAAC AGGAGACACT 
CTACGAATTG ATAAAATTAC TGTAGATGAT GGTGGACGAT ATATTTGCCA GGCTGCCACT 
TCATTCAGAC CTTCAGGTTC TAACATCCAA ACTGAAGCTA CAGGAAATGG AACAGTTACA 
GTTCGAATCA AACATAAACC TGGCCAGGCT GAAATCCATC CTTCTCAACC TGTAGCCATT 
GCTGGTCGAC CGTTTACACT CTCTTGCACA GCACACCCTC TTGGATGGCC AGTACCTGAA 
TACAGGTGGT GGAAAGAAGG CCAGCAGAAA GAAGACCTTA CTCAGAAACC GAATTATACC 
CTAATATCAG CTCACATGAG CCATGAAGGT AGATATTTCT GCCAGCCTTT TAATAGTCTT 
GGAAGGGGTG GATCGGCATC TGTGTATCTA ACTGTGCATG AACCAGCATC TATCCTCATA 
CCACTTAGAC CTCAAACCAT CAAAAAGGAC AAAGAAAGAG ATTACAGTAT AACATGCAGA 
GCTCGTGGAA AGCCCAAACC AAAAGTCAGG TGGTTACAGA ATGGTCAAGA AATATCTGAA 
GATAGCGGGC TGTTCCATAT TTCAAATTCT GATAATGTAG AGGACAATAA TGCATTCACC 
GTACAAAGTA CTCTGTCGTT TACGGGACCC AGTCGTCGAG AGAATGGCCT CACTCCTGGC 
GACAGAGGAA GATATACTTG TGAATTTAAT AATGGATTTG GCGAGAATGC TAGGTCTGAA 
ATGATGCTCA GAATTGAACA TTCCCCTGTG GTCCGACATA CGTACAATCG AGTGGCTTTT 
GATGTGGGAG AGACCGCTGT TTTGCAGTGC AAAATGCAGG CTTACCCTAG CCCAACATTT 
GAATGGGTTT CGAGAGGACG TGTTCTGGAC GAATATGGGA ACTATGGTAC GAACATCACA 
GACCTAGGCG AAGACATATA TGCTGGCGTT CTCTCTATTC GAGACATGAA GGAAGAAGAC 
TACGGAGAGT ACACGTGTAG AGCTTCCAAT CAAGTTGGGG ATGACAAGAA AACCATTATC 
AGGCTTGTTA GGAAAGGTCC TCCTGAGAGG CCTACTCACT TAGAGTTGAT AGAAGTTAGT 
TCAGATAAAG TGACTTTGAG ATGGCAGGAA GGATTCAATG GTGGTTTCGC CAACACTGAG 
TTTTTGGTTA CGTATCTCAA TACTGAAAAT GGTCGACAAA TGAATGAGTC CTGTCGGACT 
CAAAATTTGT GCCAAATCAC TGGTCTCTCT GCAAATACAG AATATCACTT CAAAGTTTTG 
GCTGTTAATC CCCGTGGTTA TAGTCCATAT TCAGAAGAGC TGTCTGTTAC AACTAAAGTG 
AATTTAAGAG ACATGCCAAG ACCAACAGAA GCACAATATG ATCGTGATAC TGGCTATCTT 
ACATTTCATG TTGAATTAAG TTCATTAAAA TTGTTGGCGA AGATTGAGGC TAGACCTGTG 
GGATCAGAGA TGTGGGTCCT ACAGACTGTG ATCTCGGTCA TTAGACCCAT AGAGAAAGTG 
CAGTTAAGCC CTCCTGATGA GGGATTTTCT GATGTGCGTG TCAAACTTTG TCTTGATAGT 
AATGACTCTT GGTGTGGTGA TGCCAAATTG GCAAATCCTT TTGAAGAAGA GCTTCCAAAT 
CCCAAAGATT CTAGAGCTGG TTTACCAACT GAGGCTGTCA TGATAAGCAT TGTTATTGGA 
GGTGTTGTAT TTTTATTGGT TATCATTTTA AGCATTTGCT GCTGTTGGAA GAAGAGAGTA 
AATCACAAAG AAAAGAAAGA TTTTGAGATG GAAACAACAA ATCCTAGACC AAAGGCTGTG 
ACGCCACCTT ACTTCCCAGA CGGGGTTGTG AACAAGGGAT TGGAGAGCGT GACGGACGAC 
ATGACAAAAG CAGGTATCTA CACCACCAAT ACATCTGGCA TCAATGGAGG GATCAATGGC 
CATATACCTT CTGGCAGTCA CCTCAATTCT GGAATGATGT ATAATCCAGA TCCTGATACT 
AGTCCCTGTA GTGCCCTAGA TCATTCAGAA CCCTGGTTGA AACCAACAGA CGAGCTTGGA 
ACAGAAGGTT CCTATCAGCC TTATGATTCT AGGTTACCTA ATGGTTATTT TTACCCAGAA 
GATTATCAAC CTTTAACAGA AGATTTAATG AACATTAAAA ATAGAGACCA TATACATTCA 
CCATACTACG ATGTGAGTGG TCTGCCGGAT CCATACGCCA TGGGCGAGGA AGACAAACCA 
CAACACATCT CCCTCTCGTA CGACGAGAGT CATGAAAGTG GCTACTCCAC ACCTAACTCT 
AGATCGAGGA GAGTCATTCG GGAAATAATT GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015923363.1
CDS	592..4107
Translation	MTSAKRTNSWITMFRCSSSTLCVCLMFILLFLCLLSTGGVKAAPQYFTDLDAEGCCSGQVMLGDSRDVVQGESLRLPCRFHSALGSSTTLYFWSRVNKDGKDNAAINAAALDPHYNIDFSPQEGKYDLLISQANYDKDNGKFECWLKEGGSGVDIYSVSYVVTVLIVPGEPRITPPKPIAREGESTALTCSSEGGSPDPTIRWYRDGVLLQGMVQKGGSRERPTSSVLTITPRLEDDDSIYRCVVWNRALREDQKLETSVSLRVHYSPRITVGPSNPLNVLENTDAHLSCNVIASPPVRSVRWLRNGMLISNTLNHTIRGVTSEDSGIYSCIAENGIGEPTQANLELSVLYGPQVRLPTYQEVAAGEPLIVKCEVLSKPPPHMIQWLKEGDPYFRQTGDTLRIDKITVDDGGRYICQAATSFRPSGSNIQTEATGNGTVTVRIKHKPGQAEIHPSQPVAIAGRPFTLSCTAHPLGWPVPEYRWWKEGQQKEDLTQKPNYTLISAHMSHEGRYFCQPFNSLGRGGSASVYLTVHEPASILIPLRPQTIKKDKERDYSITCRARGKPKPKVRWLQNGQEISEDSGLFHISNSDNVEDNNAFTVQSTLSFTGPSRRENGLTPGDRGRYTCEFNNGFGENARSEMMLRIEHSPVVRHTYNRVAFDVGETAVLQCKMQAYPSPTFEWVSRGRVLDEYGNYGTNITDLGEDIYAGVLSIRDMKEEDYGEYTCRASNQVGDDKKTIIRLVRKGPPERPTHLELIEVSSDKVTLRWQEGFNGGFANTEFLVTYLNTENGRQMNESCRTQNLCQITGLSANTEYHFKVLAVNPRGYSPYSEELSVTTKVNLRDMPRPTEAQYDRDTGYLTFHVELSSLKLLAKIEARPVGSEMWVLQTVISVIRPIEKVQLSPPDEGFSDVRVKLCLDSNDSWCGDAKLANPFEEELPNPKDSRAGLPTEAVMISIVIGGVVFLLVIILSICCCWKKRVNHKEKKDFEMETTNPRPKAVTPPYFPDGVVNKGLESVTDDMTKAGIYTTNTSGINGGINGHIPSGSHLNSGMMYNPDPDTSPCSALDHSEPWLKPTDELGTEGSYQPYDSRLPNGYFYPEDYQPLTEDLMNIKNRDHIHSPYYDVSGLPDPYAMGEEDKPQHISLSYDESHESGYSTPNSRSRRVIREIIV

Target ORF information:

RefSeq Version	XM_016067877.1
Organism	Parasteatoda tepidariorum(Common house spider)
Definition	Parasteatoda tepidariorum roundabout homolog 3-like (LOC107451685), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016067877.1

ORF Insert Sequence:

ATGACGAGTG CAAAACGGAC CAATTCCTGG ATTACAATGT TTAGGTGTTC ATCTAGTACT 
CTGTGCGTGT GCCTTATGTT TATATTATTG TTCCTGTGTT TGTTGTCAAC AGGGGGCGTA 
AAAGCTGCTC CTCAGTACTT CACCGATTTG GATGCAGAAG GTTGTTGCTC TGGGCAAGTT 
ATGCTAGGTG ATAGCAGGGA CGTAGTACAA GGTGAAAGTT TACGACTTCC CTGTAGATTT 
CATTCAGCCT TAGGATCAAG TACTACGCTC TACTTTTGGA GTAGAGTGAA TAAAGATGGT 
AAAGACAATG CAGCTATCAA TGCTGCGGCA TTGGACCCTC ATTACAATAT AGACTTCAGC 
CCTCAAGAGG GCAAATACGA TCTGCTCATA TCTCAAGCGA ATTATGACAA AGACAATGGA 
AAGTTCGAAT GCTGGTTGAA GGAAGGGGGC AGCGGAGTTG ACATCTATAG TGTTTCTTAT 
GTCGTAACTG TTTTAATTGT TCCTGGGGAA CCTAGAATAA CTCCACCAAA GCCCATAGCC 
AGGGAAGGAG AGTCCACAGC TCTTACTTGC TCAAGTGAAG GTGGAAGCCC AGATCCTACC 
ATCCGCTGGT ACAGAGATGG CGTCCTACTT CAAGGCATGG TCCAGAAAGG TGGCTCCAGG 
GAAAGGCCTA CGTCCAGCGT ACTGACCATC ACTCCTAGAC TAGAAGACGA TGATTCTATC 
TATAGATGTG TTGTTTGGAA CAGAGCTTTG AGGGAGGATC AAAAACTTGA AACAAGCGTC 
AGTCTTAGGG TTCACTATTC ACCTAGAATA ACTGTGGGGC CCTCTAACCC TTTAAACGTC 
CTAGAAAATA CTGATGCTCA TTTAAGCTGC AATGTGATAG CATCACCCCC TGTTCGTTCT 
GTCAGGTGGT TGAGGAATGG AATGCTGATA TCTAACACTC TCAATCATAC TATCAGAGGT 
GTCACATCTG AAGACAGTGG TATTTATAGC TGTATCGCTG AAAATGGCAT TGGAGAACCT 
ACACAAGCCA ACTTGGAATT ATCTGTGCTT TATGGCCCCC AAGTGAGACT TCCAACTTAT 
CAGGAAGTAG CTGCTGGCGA ACCTCTCATA GTTAAATGTG AAGTCTTGTC TAAACCTCCT 
CCTCATATGA TCCAATGGCT GAAAGAAGGG GATCCGTACT TCAGGCAAAC AGGAGACACT 
CTACGAATTG ATAAAATTAC TGTAGATGAT GGTGGACGAT ATATTTGCCA GGCTGCCACT 
TCATTCAGAC CTTCAGGTTC TAACATCCAA ACTGAAGCTA CAGGAAATGG AACAGTTACA 
GTTCGAATCA AACATAAACC TGGCCAGGCT GAAATCCATC CTTCTCAACC TGTAGCCATT 
GCTGGTCGAC CGTTTACACT CTCTTGCACA GCACACCCTC TTGGATGGCC AGTACCTGAA 
TACAGGTGGT GGAAAGAAGG CCAGCAGAAA GAAGACCTTA CTCAGAAACC GAATTATACC 
CTAATATCAG CTCACATGAG CCATGAAGGT AGATATTTCT GCCAGCCTTT TAATAGTCTT 
GGAAGGGGTG GATCGGCATC TGTGTATCTA ACTGTGCATG AACCAGCATC TATCCTCATA 
CCACTTAGAC CTCAAACCAT CAAAAAGGAC AAAGAAAGAG ATTACAGTAT AACATGCAGA 
GCTCGTGGAA AGCCCAAACC AAAAGTCAGG TGGTTACAGA ATGGTCAAGA AATATCTGAA 
GATAGCGGGC TGTTCCATAT TTCAAATTCT GATAATGTAG AGGACAATAA TGCATTCACC 
GTACAAAGTA CTCTGTCGTT TACGGGACCC AGTCGTCGAG AGAATGGCCT CACTCCTGGC 
GACAGAGGAA GATATACTTG TGAATTTAAT AATGGATTTG GCGAGAATGC TAGGTCTGAA 
ATGATGCTCA GAATTGAACA TTCCCCTGTG GTCCGACATA CGTACAATCG AGTGGCTTTT 
GATGTGGGAG AGACCGCTGT TTTGCAGTGC AAAATGCAGG CTTACCCTAG CCCAACATTT 
GAATGGGTTT CGAGAGGACG TGTTCTGGAC GAATATGGGA ACTATGGTAC GAACATCACA 
GACCTAGGCG AAGACATATA TGCTGGCGTT CTCTCTATTC GAGACATGAA GGAAGAAGAC 
TACGGAGAGT ACACGTGTAG AGCTTCCAAT CAAGTTGGGG ATGACAAGAA AACCATTATC 
AGGCTTGTTA GGAAAGGTCC TCCTGAGAGG CCTACTCACT TAGAGTTGAT AGAAGTTAGT 
TCAGATAAAG TGACTTTGAG ATGGCAGGAA GGATTCAATG GTGGTTTCGC CAACACTGAG 
TTTTTGGTTA CGTATCTCAA TACTGAAAAT GGTCGACAAA TGAATGAGTC CTGTCGGACT 
CAAAATTTGT GCCAAATCAC TGGTCTCTCT GCAAATACAG AATATCACTT CAAAGTTTTG 
GCTGTTAATC CCCGTGGTTA TAGTCCATAT TCAGAAGAGC TGTCTGTTAC AACTAAAGTG 
AATTTAAGAG ACATGCCAAG ACCAACAGAA GCACAATATG ATCGTGATAC TGGCTATCTT 
ACATTTCATG TTGAATTAAG TTCATTAAAA TTGTTGGCGA AGATTGAGGC TAGACCTGTG 
GGATCAGAGA TGTGGGTCCT ACAGACTGTG ATCTCGGTCA TTAGACCCAT AGAGAAAGTG 
CAGTTAAGCC CTCCTGATGA GGGATTTTCT GATGTGCGTG TCAAACTTTG TCTTGATAGT 
AATGACTCTT GGTGTGGTGA TGCCAAATTG GCAAATCCTT TTGAAGAAGA GCTTCCAAAT 
CCCAAAGATT CTAGAGCTGG TTTACCAACT GAGGCTGTCA TGATAAGCAT TGTTATTGGA 
GGTGTTGTAT TTTTATTGGT TATCATTTTA AGCATTTGCT GCTGTTGGAA GAAGAGAGTA 
AATCACAAAG AAAAGAAAGA TTTTGAGATG GAAACAACAA ATCCTAGACC AAAGGCTGTG 
ACGCCACCTT ACTTCCCAGA CGGGGTTGTG AACAAGGGAT TGGAGAGCGT GACGGACGAC 
ATGACAAAAG CAGGTATCTA CACCACCAAT ACATCTGGCA TCAATGGAGG GATCAATGGC 
CATATACCTT CTGGCAGTCA CCTCAATTCT GGAATGATGT ATAATCCAGA TCCTGATACT 
AGTCCCTGTA GTGCCCTAGA TCATTCAGAA CCCTGGTTGA AACCAACAGA CGAGCTTGGA 
ACAGAAGGTT CCTATCAGCC TTATGATTCT AGGTTACCTA ATGGTTATTT TTACCCAGAA 
GATTATCAAC CTTTAACAGA AGATTTAATG AACATTAAAA ATAGAGACCA TATACATTCA 
CCATACTACG ATGTGAGTGG TCTGCCGGAT CCATACGCCA TGGGCGAGGA AGACAAACCA 
CAACACATCT CCCTCTCGTA CGACGAGAGT CATGAAAGTG GCTACTCCAC ACCTAACTCT 
AGATCGAGGA GAGTCATTCG GGAAATAATT GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107451685 ( XM_016067877.2 ) cDNA ORF clone, Parasteatoda tepidariorum(common house spider) -> XP_015923363.1 Parasteatoda tepidariorum roundabout homolog 3 (LOC107451685), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OPa120369
Clone ID Related Accession (Same CDS sequence)	XM_016067877.1 , XM_016067877.2
Accession Version	XM_016067877.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3516bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-14
Organism	Parasteatoda tepidariorum(common house spider)
Product	hemicentin-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018372425.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 15, 2017 this sequence version replaced XM_016067877.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Parasteatoda tepidariorum Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGAGTG CAAAACGGAC CAATTCCTGG ATTACAATGT TTAGGTGTTC ATCTAGTACT 
CTGTGCGTGT GCCTTATGTT TATATTATTG TTCCTGTGTT TGTTGTCAAC AGGGGGCGTA 
AAAGCTGCTC CTCAGTACTT CACCGATTTG GATGCAGAAG GTTGTTGCTC TGGGCAAGTT 
ATGCTAGGTG ATAGCAGGGA CGTAGTACAA GGTGAAAGTT TACGACTTCC CTGTAGATTT 
CATTCAGCCT TAGGATCAAG TACTACGCTC TACTTTTGGA GTAGAGTGAA TAAAGATGGT 
AAAGACAATG CAGCTATCAA TGCTGCGGCA TTGGACCCTC ATTACAATAT AGACTTCAGC 
CCTCAAGAGG GCAAATACGA TCTGCTCATA TCTCAAGCGA ATTATGACAA AGACAATGGA 
AAGTTCGAAT GCTGGTTGAA GGAAGGGGGC AGCGGAGTTG ACATCTATAG TGTTTCTTAT 
GTCGTAACTG TTTTAATTGT TCCTGGGGAA CCTAGAATAA CTCCACCAAA GCCCATAGCC 
AGGGAAGGAG AGTCCACAGC TCTTACTTGC TCAAGTGAAG GTGGAAGCCC AGATCCTACC 
ATCCGCTGGT ACAGAGATGG CGTCCTACTT CAAGGCATGG TCCAGAAAGG TGGCTCCAGG 
GAAAGGCCTA CGTCCAGCGT ACTGACCATC ACTCCTAGAC TAGAAGACGA TGATTCTATC 
TATAGATGTG TTGTTTGGAA CAGAGCTTTG AGGGAGGATC AAAAACTTGA AACAAGCGTC 
AGTCTTAGGG TTCACTATTC ACCTAGAATA ACTGTGGGGC CCTCTAACCC TTTAAACGTC 
CTAGAAAATA CTGATGCTCA TTTAAGCTGC AATGTGATAG CATCACCCCC TGTTCGTTCT 
GTCAGGTGGT TGAGGAATGG AATGCTGATA TCTAACACTC TCAATCATAC TATCAGAGGT 
GTCACATCTG AAGACAGTGG TATTTATAGC TGTATCGCTG AAAATGGCAT TGGAGAACCT 
ACACAAGCCA ACTTGGAATT ATCTGTGCTT TATGGCCCCC AAGTGAGACT TCCAACTTAT 
CAGGAAGTAG CTGCTGGCGA ACCTCTCATA GTTAAATGTG AAGTCTTGTC TAAACCTCCT 
CCTCATATGA TCCAATGGCT GAAAGAAGGG GATCCGTACT TCAGGCAAAC AGGAGACACT 
CTACGAATTG ATAAAATTAC TGTAGATGAT GGTGGACGAT ATATTTGCCA GGCTGCCACT 
TCATTCAGAC CTTCAGGTTC TAACATCCAA ACTGAAGCTA CAGGAAATGG AACAGTTACA 
GTTCGAATCA AACATAAACC TGGCCAGGCT GAAATCCATC CTTCTCAACC TGTAGCCATT 
GCTGGTCGAC CGTTTACACT CTCTTGCACA GCACACCCTC TTGGATGGCC AGTACCTGAA 
TACAGGTGGT GGAAAGAAGG CCAGCAGAAA GAAGACCTTA CTCAGAAACC GAATTATACC 
CTAATATCAG CTCACATGAG CCATGAAGGT AGATATTTCT GCCAGCCTTT TAATAGTCTT 
GGAAGGGGTG GATCGGCATC TGTGTATCTA ACTGTGCATG AACCAGCATC TATCCTCATA 
CCACTTAGAC CTCAAACCAT CAAAAAGGAC AAAGAAAGAG ATTACAGTAT AACATGCAGA 
GCTCGTGGAA AGCCCAAACC AAAAGTCAGG TGGTTACAGA ATGGTCAAGA AATATCTGAA 
GATAGCGGGC TGTTCCATAT TTCAAATTCT GATAATGTAG AGGACAATAA TGCATTCACC 
GTACAAAGTA CTCTGTCGTT TACGGGACCC AGTCGTCGAG AGAATGGCCT CACTCCTGGC 
GACAGAGGAA GATATACTTG TGAATTTAAT AATGGATTTG GCGAGAATGC TAGGTCTGAA 
ATGATGCTCA GAATTGAACA TTCCCCTGTG GTCCGACATA CGTACAATCG AGTGGCTTTT 
GATGTGGGAG AGACCGCTGT TTTGCAGTGC AAAATGCAGG CTTACCCTAG CCCAACATTT 
GAATGGGTTT CGAGAGGACG TGTTCTGGAC GAATATGGGA ACTATGGTAC GAACATCACA 
GACCTAGGCG AAGACATATA TGCTGGCGTT CTCTCTATTC GAGACATGAA GGAAGAAGAC 
TACGGAGAGT ACACGTGTAG AGCTTCCAAT CAAGTTGGGG ATGACAAGAA AACCATTATC 
AGGCTTGTTA GGAAAGGTCC TCCTGAGAGG CCTACTCACT TAGAGTTGAT AGAAGTTAGT 
TCAGATAAAG TGACTTTGAG ATGGCAGGAA GGATTCAATG GTGGTTTCGC CAACACTGAG 
TTTTTGGTTA CGTATCTCAA TACTGAAAAT GGTCGACAAA TGAATGAGTC CTGTCGGACT 
CAAAATTTGT GCCAAATCAC TGGTCTCTCT GCAAATACAG AATATCACTT CAAAGTTTTG 
GCTGTTAATC CCCGTGGTTA TAGTCCATAT TCAGAAGAGC TGTCTGTTAC AACTAAAGTG 
AATTTAAGAG ACATGCCAAG ACCAACAGAA GCACAATATG ATCGTGATAC TGGCTATCTT 
ACATTTCATG TTGAATTAAG TTCATTAAAA TTGTTGGCGA AGATTGAGGC TAGACCTGTG 
GGATCAGAGA TGTGGGTCCT ACAGACTGTG ATCTCGGTCA TTAGACCCAT AGAGAAAGTG 
CAGTTAAGCC CTCCTGATGA GGGATTTTCT GATGTGCGTG TCAAACTTTG TCTTGATAGT 
AATGACTCTT GGTGTGGTGA TGCCAAATTG GCAAATCCTT TTGAAGAAGA GCTTCCAAAT 
CCCAAAGATT CTAGAGCTGG TTTACCAACT GAGGCTGTCA TGATAAGCAT TGTTATTGGA 
GGTGTTGTAT TTTTATTGGT TATCATTTTA AGCATTTGCT GCTGTTGGAA GAAGAGAGTA 
AATCACAAAG AAAAGAAAGA TTTTGAGATG GAAACAACAA ATCCTAGACC AAAGGCTGTG 
ACGCCACCTT ACTTCCCAGA CGGGGTTGTG AACAAGGGAT TGGAGAGCGT GACGGACGAC 
ATGACAAAAG CAGGTATCTA CACCACCAAT ACATCTGGCA TCAATGGAGG GATCAATGGC 
CATATACCTT CTGGCAGTCA CCTCAATTCT GGAATGATGT ATAATCCAGA TCCTGATACT 
AGTCCCTGTA GTGCCCTAGA TCATTCAGAA CCCTGGTTGA AACCAACAGA CGAGCTTGGA 
ACAGAAGGTT CCTATCAGCC TTATGATTCT AGGTTACCTA ATGGTTATTT TTACCCAGAA 
GATTATCAAC CTTTAACAGA AGATTTAATG AACATTAAAA ATAGAGACCA TATACATTCA 
CCATACTACG ATGTGAGTGG TCTGCCGGAT CCATACGCCA TGGGCGAGGA AGACAAACCA 
CAACACATCT CCCTCTCGTA CGACGAGAGT CATGAAAGTG GCTACTCCAC ACCTAACTCT 
AGATCGAGGA GAGTCATTCG GGAAATAATT GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015923363.1
CDS	593..4108
Translation	MTSAKRTNSWITMFRCSSSTLCVCLMFILLFLCLLSTGGVKAAPQYFTDLDAEGCCSGQVMLGDSRDVVQGESLRLPCRFHSALGSSTTLYFWSRVNKDGKDNAAINAAALDPHYNIDFSPQEGKYDLLISQANYDKDNGKFECWLKEGGSGVDIYSVSYVVTVLIVPGEPRITPPKPIAREGESTALTCSSEGGSPDPTIRWYRDGVLLQGMVQKGGSRERPTSSVLTITPRLEDDDSIYRCVVWNRALREDQKLETSVSLRVHYSPRITVGPSNPLNVLENTDAHLSCNVIASPPVRSVRWLRNGMLISNTLNHTIRGVTSEDSGIYSCIAENGIGEPTQANLELSVLYGPQVRLPTYQEVAAGEPLIVKCEVLSKPPPHMIQWLKEGDPYFRQTGDTLRIDKITVDDGGRYICQAATSFRPSGSNIQTEATGNGTVTVRIKHKPGQAEIHPSQPVAIAGRPFTLSCTAHPLGWPVPEYRWWKEGQQKEDLTQKPNYTLISAHMSHEGRYFCQPFNSLGRGGSASVYLTVHEPASILIPLRPQTIKKDKERDYSITCRARGKPKPKVRWLQNGQEISEDSGLFHISNSDNVEDNNAFTVQSTLSFTGPSRRENGLTPGDRGRYTCEFNNGFGENARSEMMLRIEHSPVVRHTYNRVAFDVGETAVLQCKMQAYPSPTFEWVSRGRVLDEYGNYGTNITDLGEDIYAGVLSIRDMKEEDYGEYTCRASNQVGDDKKTIIRLVRKGPPERPTHLELIEVSSDKVTLRWQEGFNGGFANTEFLVTYLNTENGRQMNESCRTQNLCQITGLSANTEYHFKVLAVNPRGYSPYSEELSVTTKVNLRDMPRPTEAQYDRDTGYLTFHVELSSLKLLAKIEARPVGSEMWVLQTVISVIRPIEKVQLSPPDEGFSDVRVKLCLDSNDSWCGDAKLANPFEEELPNPKDSRAGLPTEAVMISIVIGGVVFLLVIILSICCCWKKRVNHKEKKDFEMETTNPRPKAVTPPYFPDGVVNKGLESVTDDMTKAGIYTTNTSGINGGINGHIPSGSHLNSGMMYNPDPDTSPCSALDHSEPWLKPTDELGTEGSYQPYDSRLPNGYFYPEDYQPLTEDLMNIKNRDHIHSPYYDVSGLPDPYAMGEEDKPQHISLSYDESHESGYSTPNSRSRRVIREIIV

Target ORF information:

RefSeq Version	XM_016067877.2
Organism	Parasteatoda tepidariorum(common house spider)
Definition	Parasteatoda tepidariorum roundabout homolog 3 (LOC107451685), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016067877.2

ORF Insert Sequence:

ATGACGAGTG CAAAACGGAC CAATTCCTGG ATTACAATGT TTAGGTGTTC ATCTAGTACT 
CTGTGCGTGT GCCTTATGTT TATATTATTG TTCCTGTGTT TGTTGTCAAC AGGGGGCGTA 
AAAGCTGCTC CTCAGTACTT CACCGATTTG GATGCAGAAG GTTGTTGCTC TGGGCAAGTT 
ATGCTAGGTG ATAGCAGGGA CGTAGTACAA GGTGAAAGTT TACGACTTCC CTGTAGATTT 
CATTCAGCCT TAGGATCAAG TACTACGCTC TACTTTTGGA GTAGAGTGAA TAAAGATGGT 
AAAGACAATG CAGCTATCAA TGCTGCGGCA TTGGACCCTC ATTACAATAT AGACTTCAGC 
CCTCAAGAGG GCAAATACGA TCTGCTCATA TCTCAAGCGA ATTATGACAA AGACAATGGA 
AAGTTCGAAT GCTGGTTGAA GGAAGGGGGC AGCGGAGTTG ACATCTATAG TGTTTCTTAT 
GTCGTAACTG TTTTAATTGT TCCTGGGGAA CCTAGAATAA CTCCACCAAA GCCCATAGCC 
AGGGAAGGAG AGTCCACAGC TCTTACTTGC TCAAGTGAAG GTGGAAGCCC AGATCCTACC 
ATCCGCTGGT ACAGAGATGG CGTCCTACTT CAAGGCATGG TCCAGAAAGG TGGCTCCAGG 
GAAAGGCCTA CGTCCAGCGT ACTGACCATC ACTCCTAGAC TAGAAGACGA TGATTCTATC 
TATAGATGTG TTGTTTGGAA CAGAGCTTTG AGGGAGGATC AAAAACTTGA AACAAGCGTC 
AGTCTTAGGG TTCACTATTC ACCTAGAATA ACTGTGGGGC CCTCTAACCC TTTAAACGTC 
CTAGAAAATA CTGATGCTCA TTTAAGCTGC AATGTGATAG CATCACCCCC TGTTCGTTCT 
GTCAGGTGGT TGAGGAATGG AATGCTGATA TCTAACACTC TCAATCATAC TATCAGAGGT 
GTCACATCTG AAGACAGTGG TATTTATAGC TGTATCGCTG AAAATGGCAT TGGAGAACCT 
ACACAAGCCA ACTTGGAATT ATCTGTGCTT TATGGCCCCC AAGTGAGACT TCCAACTTAT 
CAGGAAGTAG CTGCTGGCGA ACCTCTCATA GTTAAATGTG AAGTCTTGTC TAAACCTCCT 
CCTCATATGA TCCAATGGCT GAAAGAAGGG GATCCGTACT TCAGGCAAAC AGGAGACACT 
CTACGAATTG ATAAAATTAC TGTAGATGAT GGTGGACGAT ATATTTGCCA GGCTGCCACT 
TCATTCAGAC CTTCAGGTTC TAACATCCAA ACTGAAGCTA CAGGAAATGG AACAGTTACA 
GTTCGAATCA AACATAAACC TGGCCAGGCT GAAATCCATC CTTCTCAACC TGTAGCCATT 
GCTGGTCGAC CGTTTACACT CTCTTGCACA GCACACCCTC TTGGATGGCC AGTACCTGAA 
TACAGGTGGT GGAAAGAAGG CCAGCAGAAA GAAGACCTTA CTCAGAAACC GAATTATACC 
CTAATATCAG CTCACATGAG CCATGAAGGT AGATATTTCT GCCAGCCTTT TAATAGTCTT 
GGAAGGGGTG GATCGGCATC TGTGTATCTA ACTGTGCATG AACCAGCATC TATCCTCATA 
CCACTTAGAC CTCAAACCAT CAAAAAGGAC AAAGAAAGAG ATTACAGTAT AACATGCAGA 
GCTCGTGGAA AGCCCAAACC AAAAGTCAGG TGGTTACAGA ATGGTCAAGA AATATCTGAA 
GATAGCGGGC TGTTCCATAT TTCAAATTCT GATAATGTAG AGGACAATAA TGCATTCACC 
GTACAAAGTA CTCTGTCGTT TACGGGACCC AGTCGTCGAG AGAATGGCCT CACTCCTGGC 
GACAGAGGAA GATATACTTG TGAATTTAAT AATGGATTTG GCGAGAATGC TAGGTCTGAA 
ATGATGCTCA GAATTGAACA TTCCCCTGTG GTCCGACATA CGTACAATCG AGTGGCTTTT 
GATGTGGGAG AGACCGCTGT TTTGCAGTGC AAAATGCAGG CTTACCCTAG CCCAACATTT 
GAATGGGTTT CGAGAGGACG TGTTCTGGAC GAATATGGGA ACTATGGTAC GAACATCACA 
GACCTAGGCG AAGACATATA TGCTGGCGTT CTCTCTATTC GAGACATGAA GGAAGAAGAC 
TACGGAGAGT ACACGTGTAG AGCTTCCAAT CAAGTTGGGG ATGACAAGAA AACCATTATC 
AGGCTTGTTA GGAAAGGTCC TCCTGAGAGG CCTACTCACT TAGAGTTGAT AGAAGTTAGT 
TCAGATAAAG TGACTTTGAG ATGGCAGGAA GGATTCAATG GTGGTTTCGC CAACACTGAG 
TTTTTGGTTA CGTATCTCAA TACTGAAAAT GGTCGACAAA TGAATGAGTC CTGTCGGACT 
CAAAATTTGT GCCAAATCAC TGGTCTCTCT GCAAATACAG AATATCACTT CAAAGTTTTG 
GCTGTTAATC CCCGTGGTTA TAGTCCATAT TCAGAAGAGC TGTCTGTTAC AACTAAAGTG 
AATTTAAGAG ACATGCCAAG ACCAACAGAA GCACAATATG ATCGTGATAC TGGCTATCTT 
ACATTTCATG TTGAATTAAG TTCATTAAAA TTGTTGGCGA AGATTGAGGC TAGACCTGTG 
GGATCAGAGA TGTGGGTCCT ACAGACTGTG ATCTCGGTCA TTAGACCCAT AGAGAAAGTG 
CAGTTAAGCC CTCCTGATGA GGGATTTTCT GATGTGCGTG TCAAACTTTG TCTTGATAGT 
AATGACTCTT GGTGTGGTGA TGCCAAATTG GCAAATCCTT TTGAAGAAGA GCTTCCAAAT 
CCCAAAGATT CTAGAGCTGG TTTACCAACT GAGGCTGTCA TGATAAGCAT TGTTATTGGA 
GGTGTTGTAT TTTTATTGGT TATCATTTTA AGCATTTGCT GCTGTTGGAA GAAGAGAGTA 
AATCACAAAG AAAAGAAAGA TTTTGAGATG GAAACAACAA ATCCTAGACC AAAGGCTGTG 
ACGCCACCTT ACTTCCCAGA CGGGGTTGTG AACAAGGGAT TGGAGAGCGT GACGGACGAC 
ATGACAAAAG CAGGTATCTA CACCACCAAT ACATCTGGCA TCAATGGAGG GATCAATGGC 
CATATACCTT CTGGCAGTCA CCTCAATTCT GGAATGATGT ATAATCCAGA TCCTGATACT 
AGTCCCTGTA GTGCCCTAGA TCATTCAGAA CCCTGGTTGA AACCAACAGA CGAGCTTGGA 
ACAGAAGGTT CCTATCAGCC TTATGATTCT AGGTTACCTA ATGGTTATTT TTACCCAGAA 
GATTATCAAC CTTTAACAGA AGATTTAATG AACATTAAAA ATAGAGACCA TATACATTCA 
CCATACTACG ATGTGAGTGG TCTGCCGGAT CCATACGCCA TGGGCGAGGA AGACAAACCA 
CAACACATCT CCCTCTCGTA CGACGAGAGT CATGAAAGTG GCTACTCCAC ACCTAACTCT 
AGATCGAGGA GAGTCATTCG GGAAATAATT GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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電話

03-6811-6572

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オンライン注文

LOC107451685 cDNA ORF clone, Parasteatoda tepidariorum(common house spider)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム