LOC107438324 cDNA ORF clone, Parasteatoda tepidariorum(common house spider)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC107438324
Entrez Gene ID	107438324
Full Name	symplekin
Gene Type	protein-coding
Organism	Parasteatoda tepidariorum(common house spider)

mRNA and Protein(s)

mRNA	Protein	Name
XM_016050601.1	XP_015906087.1	symplekin isoform X2
XM_016050592.1	XP_015906078.1	symplekin isoform X1

Pathways from BioSystems
Gene Ontology

The following LOC107438324 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC107438324 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPa119100	XM_016050592.1 Latest version!	Parasteatoda tepidariorum symplekin (LOC107438324), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OPa119101	XM_016050601.1 Latest version!	Parasteatoda tepidariorum symplekin (LOC107438324), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC107438324 ( XM_016050592.1 ) cDNA ORF clone, Parasteatoda tepidariorum(common house spider) -> XP_015906078.1 Parasteatoda tepidariorum symplekin (LOC107438324), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPa119100
Clone ID Related Accession (Same CDS sequence)	XM_016050592.1
Accession Version	XM_016050592.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3459bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-14
Organism	Parasteatoda tepidariorum(common house spider)
Product	symplekin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018370773.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Parasteatoda tepidariorum Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTCAA CCAGGAAAAG TTCGAGACAT TCCTCATCTT CTAAGGAAGA GTATAAACGA 
TATTCTTCTT CAAGTAGAAG CTCTACTAGA ACGGAAACAA CTGAAGCTCA GAAAAGCTCT 
CTCCCCTCCA AAAGAAAAGC TGAAGCAGAA ACAGATTTGG TTCAAAAATT AAAAAAGAAA 
ACTGTAGAAT GTCGACGTAG CACTGCTGCT CAATTTTTTG TTGAAGAAGA AACTGTACAA 
GAATCAGTGA CTGCTGATCG AATTGTCCAG CTCCTCAATG ATGCTGTTAC ACCAAAGGAA 
GAAAATGGAA AGCTGAAAAA TCTGCTCATT GTCAAAGAAC TGATCATACA GAAAGAACCT 
AAGTTGCTGG ACAACTTTCT GGATGAAGTA GTAAGTTTTC AACATGACAC TGATCCTGAA 
GTACGCAGAT TCATTGTACA ATTCATTGAG GATGTTTGTA GGAGAGACTT ACAATTTCTT 
CCAAAAGTAA TCAACAATCT GTTGATGATG CTACACGATG AATCGGTCAA AGTTCAGAAA 
AAAGTTATCC AAACAACAAC TCATCTTTAT CCTCTAACCA TTCGTTATTT TGTAAATGCA 
AAACATGTTG ATGATGTAAT GGAAGCTATT TGGGCTTCAA TGACAGAACT AAAATTAACA 
ATTGTCTCGC TCTTGGAGTC TGACAATGAA GGAGTCCGTA CTTCAGCTAC AAAATTTATG 
GAACATGTTA TTCTTGCACA AACATTCAAA GACCAATATG CAGATCCGAA AAACACTGAT 
TTATCTTTGG ATGAGATACC AGTGATATTA AAAGTTTTTC GACCCAGGAA GATGGAAGAA 
GAAGCCAAAG ATATTTTCCA GCGAATTGTT GCTTTTCATT GTGCTTCCCA TGTGTCTCTT 
TGTAACCTTA TGGCTTGCAT GCAAGTTTTA ACTTCAATAG GAAGGCAAAG ATTTGAGTTT 
TTTCCTAAAG TTTTAGAAGC ATTTGAATTG CTTCATGCAA ATCTGCCACC AACGTTGGGT 
CAATCACAAG TGAGTAGTGT AAGAAAGAAT TTAAAATTAG AGCTCTTTCA ATTGGTTAAG 
CATCCTGGCG CTGTCGAATT TCAATCAAGA ATATCAATCA TGCTCTCTGA TTTAGGTGCA 
TCACCTCAGC AGATAAGTAG ATGCTTTCCG GATGCAGAGG CAATCAAGAA ACCTAGCGTC 
AAAATAGAGG ACAGAAGTGC AAAACAGGAA GATATACCAT CTTCATCAAG ACCTCGCAAA 
ATAAAATCCA GTCTTGAGAA AGGAACTAAA GCTAAAAACA CAGCTATTGA TATTACAGCT 
GAGGATTTAG TACCCAAATT TCACAGTATA AATGTTGTTG ATTTAGTATT AGTAAGCATG 
TTAAGTTTGC CTGATGTTAT GCCTACTAAC TTTCAGTCCA CATACACTCC TATTGCAGCT 
GCTGGCACCG AAGCTCAGAT TAAACATCTG GCTCGTCTTT TAGCCACACA ATTAACTTTG 
GCTGGAATTG GAAAAGGTGT GGAACAAATG AAACATAAAA TGGAAAATGA AAAAGTGGAA 
AGTGATGAGG ATGAGGCAGC CTCTAATGTA CCCAAACCAA TCCAAACTCT AGTAAGCCAA 
ACTACAGAAA TGAAGCCTAA AAAGCAAGGG CTTGTTTTAA TGCCTAGTGG TGGCTCCCAA 
ATAAAAGCTA ATAGAATATC TAAGAAAATC GACTTCCAAG CCATAGCGAA ATCAATGAAC 
GAAGAAGAGT CTAATAATTT TTGTAGAGCT GCTTATAATC GAATCTTGAA TGCTGAAAAA 
ATGATTACTA AAAGAGGTGC TGCTCCAATT AGAACAAAAT TGCTTACTAA CTTAGCTACC 
CGATTTAGAG GTGATTTTTC CATTGATTTG AAAAATCATA TCATGGAAAA TCCCCGCAAT 
AGATTAGATC TAGCATTTTC TTGGATATAT CAAGAATATT CTGCTTATAG AGGATTTGAA 
TGTTCCATAG AAAGCCATGT TGAAAATTAT GAAGAAGCAG TCGATTCTCT ATTGGGTGGC 
ATTTTGAAGA GTGACGAAGA AAACCATGCT CTTTACACTC GTTTTTTCTT GGATGTTCCA 
TTTATTACAG AAGGGATGGC TGAGTCTCTG AAAACTGTTT GTATTGATGA ATCATCTACT 
GCTGAAGCTC TTGAAATGGC TGAAAGCATG AGTTTTAGAA GACCTACAAA GAAGTTGCTG 
TTTTTAGGAG TAATATGTGA CCTTACTCTT CATGAAAATT GTGACATACG GACAAAAGCT 
GTAAAAGCAG CAGTTGACAT CCATGAAAGT GGCCATATGA GGTCATTCAT TGAAGCTTTT 
GCTTTAAGAA GTCTCAAGTT TTTGTTACAT ACTGACCCAC CAACTGATTT CTTCCTGTTT 
TTCAAACAAA GGCCTGTTAC TTGGTCTGAA GATTTGACTA AAGTATGTTT ACATTTGTAT 
TTAACTTTGT TGCCCATAAA CCATCGCCTC ATTCAAGATC TTGGAACTGT TTATGTTGAA 
GCCACTGGTG ATATTAAAAG AATTATTCTC CGTGCCCTCG AAGCACCAGT TAAAGGCATG 
AGCATGGCCT CTCCTGAACT CTTACTCTTT GTTGAAAACT GTCCTAAAGG AGCCGAGACA 
TTAATTACAA GAATCATCCA TATTTTGACT GACAAAGCAC CACCATCAGC CGAACTTGTT 
GCTAGAGTTC GAGATTTATA TCGTAAAAGA GTTCCAGACG TACGTTTCTT TATTCCAATC 
ATTAATGGTC TTTCTAAGCG TGAAATAATT TCTGCATTAC CTGAATTGAT TCAACAAAAA 
ACACCAGTAG TAAAAGAAGT TTTGCATCGT CTTTTAGGAA GTAATTTTTC TAGCCCGATA 
TCTCCAGCTG AACTATTGGT CGCTCTGCAT AACATTGATC CTTCTAAATG CAACCTTAAA 
ATAATTATCA AAGCTACTTC ACTCTGTTTG GAAGAGAAAA ATGTTTACAC TCAAGTTGTC 
TTGGCGATGG TTATTCAGGA ACTGATGGAA CAAACTCCTT TGCCTATTTT ATTAATGAGA 
ACTGTTATAC AATCTTTAGC AAACTATCCT CATCTTTTGG ATTTTGTTAC TCACATATTG 
CAACGGTTAA TATTGAAAGA AGTCTGGAAA CAGAAGAAAA TTTGGGAGGG TTTTATTAAG 
TGCTGCCAAA GAACTAAACC AAAATCTTTT CAGGTGTTGT TGCAATTGCC TGCCCCACAA 
TTGGAGGAAG TCCTAAGTTC GTGCCCAGAT TTACGTGAAT CATTATGCCT ACATGTTAAA 
TCATTTAATG AGGGACAGAA GGCAAATATT CCTCAAGCTG TGATAGATGT CCTTGGTGTG 
GATTTGTCTC AAGATGATAA AAAATCCGAA GATGGCTCTA TAAAAAATGA CGAAGAAAAC 
ATTAAAGAAC CATTACCACC TGGACAAGAT CATTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015906078.1
CDS	148..3606
Translation	MDSTRKSSRHSSSSKEEYKRYSSSSRSSTRTETTEAQKSSLPSKRKAEAETDLVQKLKKKTVECRRSTAAQFFVEEETVQESVTADRIVQLLNDAVTPKEENGKLKNLLIVKELIIQKEPKLLDNFLDEVVSFQHDTDPEVRRFIVQFIEDVCRRDLQFLPKVINNLLMMLHDESVKVQKKVIQTTTHLYPLTIRYFVNAKHVDDVMEAIWASMTELKLTIVSLLESDNEGVRTSATKFMEHVILAQTFKDQYADPKNTDLSLDEIPVILKVFRPRKMEEEAKDIFQRIVAFHCASHVSLCNLMACMQVLTSIGRQRFEFFPKVLEAFELLHANLPPTLGQSQVSSVRKNLKLELFQLVKHPGAVEFQSRISIMLSDLGASPQQISRCFPDAEAIKKPSVKIEDRSAKQEDIPSSSRPRKIKSSLEKGTKAKNTAIDITAEDLVPKFHSINVVDLVLVSMLSLPDVMPTNFQSTYTPIAAAGTEAQIKHLARLLATQLTLAGIGKGVEQMKHKMENEKVESDEDEAASNVPKPIQTLVSQTTEMKPKKQGLVLMPSGGSQIKANRISKKIDFQAIAKSMNEEESNNFCRAAYNRILNAEKMITKRGAAPIRTKLLTNLATRFRGDFSIDLKNHIMENPRNRLDLAFSWIYQEYSAYRGFECSIESHVENYEEAVDSLLGGILKSDEENHALYTRFFLDVPFITEGMAESLKTVCIDESSTAEALEMAESMSFRRPTKKLLFLGVICDLTLHENCDIRTKAVKAAVDIHESGHMRSFIEAFALRSLKFLLHTDPPTDFFLFFKQRPVTWSEDLTKVCLHLYLTLLPINHRLIQDLGTVYVEATGDIKRIILRALEAPVKGMSMASPELLLFVENCPKGAETLITRIIHILTDKAPPSAELVARVRDLYRKRVPDVRFFIPIINGLSKREIISALPELIQQKTPVVKEVLHRLLGSNFSSPISPAELLVALHNIDPSKCNLKIIIKATSLCLEEKNVYTQVVLAMVIQELMEQTPLPILLMRTVIQSLANYPHLLDFVTHILQRLILKEVWKQKKIWEGFIKCCQRTKPKSFQVLLQLPAPQLEEVLSSCPDLRESLCLHVKSFNEGQKANIPQAVIDVLGVDLSQDDKKSEDGSIKNDEENIKEPLPPGQDHL

Target ORF information:

RefSeq Version	XM_016050592.1
Organism	Parasteatoda tepidariorum(common house spider)
Definition	Parasteatoda tepidariorum symplekin (LOC107438324), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016050592.1

ORF Insert Sequence:

ATGGATTCAA CCAGGAAAAG TTCGAGACAT TCCTCATCTT CTAAGGAAGA GTATAAACGA 
TATTCTTCTT CAAGTAGAAG CTCTACTAGA ACGGAAACAA CTGAAGCTCA GAAAAGCTCT 
CTCCCCTCCA AAAGAAAAGC TGAAGCAGAA ACAGATTTGG TTCAAAAATT AAAAAAGAAA 
ACTGTAGAAT GTCGACGTAG CACTGCTGCT CAATTTTTTG TTGAAGAAGA AACTGTACAA 
GAATCAGTGA CTGCTGATCG AATTGTCCAG CTCCTCAATG ATGCTGTTAC ACCAAAGGAA 
GAAAATGGAA AGCTGAAAAA TCTGCTCATT GTCAAAGAAC TGATCATACA GAAAGAACCT 
AAGTTGCTGG ACAACTTTCT GGATGAAGTA GTAAGTTTTC AACATGACAC TGATCCTGAA 
GTACGCAGAT TCATTGTACA ATTCATTGAG GATGTTTGTA GGAGAGACTT ACAATTTCTT 
CCAAAAGTAA TCAACAATCT GTTGATGATG CTACACGATG AATCGGTCAA AGTTCAGAAA 
AAAGTTATCC AAACAACAAC TCATCTTTAT CCTCTAACCA TTCGTTATTT TGTAAATGCA 
AAACATGTTG ATGATGTAAT GGAAGCTATT TGGGCTTCAA TGACAGAACT AAAATTAACA 
ATTGTCTCGC TCTTGGAGTC TGACAATGAA GGAGTCCGTA CTTCAGCTAC AAAATTTATG 
GAACATGTTA TTCTTGCACA AACATTCAAA GACCAATATG CAGATCCGAA AAACACTGAT 
TTATCTTTGG ATGAGATACC AGTGATATTA AAAGTTTTTC GACCCAGGAA GATGGAAGAA 
GAAGCCAAAG ATATTTTCCA GCGAATTGTT GCTTTTCATT GTGCTTCCCA TGTGTCTCTT 
TGTAACCTTA TGGCTTGCAT GCAAGTTTTA ACTTCAATAG GAAGGCAAAG ATTTGAGTTT 
TTTCCTAAAG TTTTAGAAGC ATTTGAATTG CTTCATGCAA ATCTGCCACC AACGTTGGGT 
CAATCACAAG TGAGTAGTGT AAGAAAGAAT TTAAAATTAG AGCTCTTTCA ATTGGTTAAG 
CATCCTGGCG CTGTCGAATT TCAATCAAGA ATATCAATCA TGCTCTCTGA TTTAGGTGCA 
TCACCTCAGC AGATAAGTAG ATGCTTTCCG GATGCAGAGG CAATCAAGAA ACCTAGCGTC 
AAAATAGAGG ACAGAAGTGC AAAACAGGAA GATATACCAT CTTCATCAAG ACCTCGCAAA 
ATAAAATCCA GTCTTGAGAA AGGAACTAAA GCTAAAAACA CAGCTATTGA TATTACAGCT 
GAGGATTTAG TACCCAAATT TCACAGTATA AATGTTGTTG ATTTAGTATT AGTAAGCATG 
TTAAGTTTGC CTGATGTTAT GCCTACTAAC TTTCAGTCCA CATACACTCC TATTGCAGCT 
GCTGGCACCG AAGCTCAGAT TAAACATCTG GCTCGTCTTT TAGCCACACA ATTAACTTTG 
GCTGGAATTG GAAAAGGTGT GGAACAAATG AAACATAAAA TGGAAAATGA AAAAGTGGAA 
AGTGATGAGG ATGAGGCAGC CTCTAATGTA CCCAAACCAA TCCAAACTCT AGTAAGCCAA 
ACTACAGAAA TGAAGCCTAA AAAGCAAGGG CTTGTTTTAA TGCCTAGTGG TGGCTCCCAA 
ATAAAAGCTA ATAGAATATC TAAGAAAATC GACTTCCAAG CCATAGCGAA ATCAATGAAC 
GAAGAAGAGT CTAATAATTT TTGTAGAGCT GCTTATAATC GAATCTTGAA TGCTGAAAAA 
ATGATTACTA AAAGAGGTGC TGCTCCAATT AGAACAAAAT TGCTTACTAA CTTAGCTACC 
CGATTTAGAG GTGATTTTTC CATTGATTTG AAAAATCATA TCATGGAAAA TCCCCGCAAT 
AGATTAGATC TAGCATTTTC TTGGATATAT CAAGAATATT CTGCTTATAG AGGATTTGAA 
TGTTCCATAG AAAGCCATGT TGAAAATTAT GAAGAAGCAG TCGATTCTCT ATTGGGTGGC 
ATTTTGAAGA GTGACGAAGA AAACCATGCT CTTTACACTC GTTTTTTCTT GGATGTTCCA 
TTTATTACAG AAGGGATGGC TGAGTCTCTG AAAACTGTTT GTATTGATGA ATCATCTACT 
GCTGAAGCTC TTGAAATGGC TGAAAGCATG AGTTTTAGAA GACCTACAAA GAAGTTGCTG 
TTTTTAGGAG TAATATGTGA CCTTACTCTT CATGAAAATT GTGACATACG GACAAAAGCT 
GTAAAAGCAG CAGTTGACAT CCATGAAAGT GGCCATATGA GGTCATTCAT TGAAGCTTTT 
GCTTTAAGAA GTCTCAAGTT TTTGTTACAT ACTGACCCAC CAACTGATTT CTTCCTGTTT 
TTCAAACAAA GGCCTGTTAC TTGGTCTGAA GATTTGACTA AAGTATGTTT ACATTTGTAT 
TTAACTTTGT TGCCCATAAA CCATCGCCTC ATTCAAGATC TTGGAACTGT TTATGTTGAA 
GCCACTGGTG ATATTAAAAG AATTATTCTC CGTGCCCTCG AAGCACCAGT TAAAGGCATG 
AGCATGGCCT CTCCTGAACT CTTACTCTTT GTTGAAAACT GTCCTAAAGG AGCCGAGACA 
TTAATTACAA GAATCATCCA TATTTTGACT GACAAAGCAC CACCATCAGC CGAACTTGTT 
GCTAGAGTTC GAGATTTATA TCGTAAAAGA GTTCCAGACG TACGTTTCTT TATTCCAATC 
ATTAATGGTC TTTCTAAGCG TGAAATAATT TCTGCATTAC CTGAATTGAT TCAACAAAAA 
ACACCAGTAG TAAAAGAAGT TTTGCATCGT CTTTTAGGAA GTAATTTTTC TAGCCCGATA 
TCTCCAGCTG AACTATTGGT CGCTCTGCAT AACATTGATC CTTCTAAATG CAACCTTAAA 
ATAATTATCA AAGCTACTTC ACTCTGTTTG GAAGAGAAAA ATGTTTACAC TCAAGTTGTC 
TTGGCGATGG TTATTCAGGA ACTGATGGAA CAAACTCCTT TGCCTATTTT ATTAATGAGA 
ACTGTTATAC AATCTTTAGC AAACTATCCT CATCTTTTGG ATTTTGTTAC TCACATATTG 
CAACGGTTAA TATTGAAAGA AGTCTGGAAA CAGAAGAAAA TTTGGGAGGG TTTTATTAAG 
TGCTGCCAAA GAACTAAACC AAAATCTTTT CAGGTGTTGT TGCAATTGCC TGCCCCACAA 
TTGGAGGAAG TCCTAAGTTC GTGCCCAGAT TTACGTGAAT CATTATGCCT ACATGTTAAA 
TCATTTAATG AGGGACAGAA GGCAAATATT CCTCAAGCTG TGATAGATGT CCTTGGTGTG 
GATTTGTCTC AAGATGATAA AAAATCCGAA GATGGCTCTA TAAAAAATGA CGAAGAAAAC 
ATTAAAGAAC CATTACCACC TGGACAAGAT CATTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107438324 ( XM_016050601.1 ) cDNA ORF clone, Parasteatoda tepidariorum(common house spider) -> XP_015906087.1 Parasteatoda tepidariorum symplekin (LOC107438324), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPa119101
Clone ID Related Accession (Same CDS sequence)	XM_016050601.1
Accession Version	XM_016050601.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3456bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-14
Organism	Parasteatoda tepidariorum(common house spider)
Product	symplekin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018370773.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Parasteatoda tepidariorum Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTCAA CCAGGAAAAG TTCGAGACAT TCCTCATCTT CTAAGGAAGA GTATAAACGA 
TATTCTTCTT CAAGTAGAAG CTCTACTAGA ACGGAAACAA CTGAAGCTCA GAAAAGCTCT 
CTCCCCTCCA AAAGAAAAGC TGAAGAAACA GATTTGGTTC AAAAATTAAA AAAGAAAACT 
GTAGAATGTC GACGTAGCAC TGCTGCTCAA TTTTTTGTTG AAGAAGAAAC TGTACAAGAA 
TCAGTGACTG CTGATCGAAT TGTCCAGCTC CTCAATGATG CTGTTACACC AAAGGAAGAA 
AATGGAAAGC TGAAAAATCT GCTCATTGTC AAAGAACTGA TCATACAGAA AGAACCTAAG 
TTGCTGGACA ACTTTCTGGA TGAAGTAGTA AGTTTTCAAC ATGACACTGA TCCTGAAGTA 
CGCAGATTCA TTGTACAATT CATTGAGGAT GTTTGTAGGA GAGACTTACA ATTTCTTCCA 
AAAGTAATCA ACAATCTGTT GATGATGCTA CACGATGAAT CGGTCAAAGT TCAGAAAAAA 
GTTATCCAAA CAACAACTCA TCTTTATCCT CTAACCATTC GTTATTTTGT AAATGCAAAA 
CATGTTGATG ATGTAATGGA AGCTATTTGG GCTTCAATGA CAGAACTAAA ATTAACAATT 
GTCTCGCTCT TGGAGTCTGA CAATGAAGGA GTCCGTACTT CAGCTACAAA ATTTATGGAA 
CATGTTATTC TTGCACAAAC ATTCAAAGAC CAATATGCAG ATCCGAAAAA CACTGATTTA 
TCTTTGGATG AGATACCAGT GATATTAAAA GTTTTTCGAC CCAGGAAGAT GGAAGAAGAA 
GCCAAAGATA TTTTCCAGCG AATTGTTGCT TTTCATTGTG CTTCCCATGT GTCTCTTTGT 
AACCTTATGG CTTGCATGCA AGTTTTAACT TCAATAGGAA GGCAAAGATT TGAGTTTTTT 
CCTAAAGTTT TAGAAGCATT TGAATTGCTT CATGCAAATC TGCCACCAAC GTTGGGTCAA 
TCACAAGTGA GTAGTGTAAG AAAGAATTTA AAATTAGAGC TCTTTCAATT GGTTAAGCAT 
CCTGGCGCTG TCGAATTTCA ATCAAGAATA TCAATCATGC TCTCTGATTT AGGTGCATCA 
CCTCAGCAGA TAAGTAGATG CTTTCCGGAT GCAGAGGCAA TCAAGAAACC TAGCGTCAAA 
ATAGAGGACA GAAGTGCAAA ACAGGAAGAT ATACCATCTT CATCAAGACC TCGCAAAATA 
AAATCCAGTC TTGAGAAAGG AACTAAAGCT AAAAACACAG CTATTGATAT TACAGCTGAG 
GATTTAGTAC CCAAATTTCA CAGTATAAAT GTTGTTGATT TAGTATTAGT AAGCATGTTA 
AGTTTGCCTG ATGTTATGCC TACTAACTTT CAGTCCACAT ACACTCCTAT TGCAGCTGCT 
GGCACCGAAG CTCAGATTAA ACATCTGGCT CGTCTTTTAG CCACACAATT AACTTTGGCT 
GGAATTGGAA AAGGTGTGGA ACAAATGAAA CATAAAATGG AAAATGAAAA AGTGGAAAGT 
GATGAGGATG AGGCAGCCTC TAATGTACCC AAACCAATCC AAACTCTAGT AAGCCAAACT 
ACAGAAATGA AGCCTAAAAA GCAAGGGCTT GTTTTAATGC CTAGTGGTGG CTCCCAAATA 
AAAGCTAATA GAATATCTAA GAAAATCGAC TTCCAAGCCA TAGCGAAATC AATGAACGAA 
GAAGAGTCTA ATAATTTTTG TAGAGCTGCT TATAATCGAA TCTTGAATGC TGAAAAAATG 
ATTACTAAAA GAGGTGCTGC TCCAATTAGA ACAAAATTGC TTACTAACTT AGCTACCCGA 
TTTAGAGGTG ATTTTTCCAT TGATTTGAAA AATCATATCA TGGAAAATCC CCGCAATAGA 
TTAGATCTAG CATTTTCTTG GATATATCAA GAATATTCTG CTTATAGAGG ATTTGAATGT 
TCCATAGAAA GCCATGTTGA AAATTATGAA GAAGCAGTCG ATTCTCTATT GGGTGGCATT 
TTGAAGAGTG ACGAAGAAAA CCATGCTCTT TACACTCGTT TTTTCTTGGA TGTTCCATTT 
ATTACAGAAG GGATGGCTGA GTCTCTGAAA ACTGTTTGTA TTGATGAATC ATCTACTGCT 
GAAGCTCTTG AAATGGCTGA AAGCATGAGT TTTAGAAGAC CTACAAAGAA GTTGCTGTTT 
TTAGGAGTAA TATGTGACCT TACTCTTCAT GAAAATTGTG ACATACGGAC AAAAGCTGTA 
AAAGCAGCAG TTGACATCCA TGAAAGTGGC CATATGAGGT CATTCATTGA AGCTTTTGCT 
TTAAGAAGTC TCAAGTTTTT GTTACATACT GACCCACCAA CTGATTTCTT CCTGTTTTTC 
AAACAAAGGC CTGTTACTTG GTCTGAAGAT TTGACTAAAG TATGTTTACA TTTGTATTTA 
ACTTTGTTGC CCATAAACCA TCGCCTCATT CAAGATCTTG GAACTGTTTA TGTTGAAGCC 
ACTGGTGATA TTAAAAGAAT TATTCTCCGT GCCCTCGAAG CACCAGTTAA AGGCATGAGC 
ATGGCCTCTC CTGAACTCTT ACTCTTTGTT GAAAACTGTC CTAAAGGAGC CGAGACATTA 
ATTACAAGAA TCATCCATAT TTTGACTGAC AAAGCACCAC CATCAGCCGA ACTTGTTGCT 
AGAGTTCGAG ATTTATATCG TAAAAGAGTT CCAGACGTAC GTTTCTTTAT TCCAATCATT 
AATGGTCTTT CTAAGCGTGA AATAATTTCT GCATTACCTG AATTGATTCA ACAAAAAACA 
CCAGTAGTAA AAGAAGTTTT GCATCGTCTT TTAGGAAGTA ATTTTTCTAG CCCGATATCT 
CCAGCTGAAC TATTGGTCGC TCTGCATAAC ATTGATCCTT CTAAATGCAA CCTTAAAATA 
ATTATCAAAG CTACTTCACT CTGTTTGGAA GAGAAAAATG TTTACACTCA AGTTGTCTTG 
GCGATGGTTA TTCAGGAACT GATGGAACAA ACTCCTTTGC CTATTTTATT AATGAGAACT 
GTTATACAAT CTTTAGCAAA CTATCCTCAT CTTTTGGATT TTGTTACTCA CATATTGCAA 
CGGTTAATAT TGAAAGAAGT CTGGAAACAG AAGAAAATTT GGGAGGGTTT TATTAAGTGC 
TGCCAAAGAA CTAAACCAAA ATCTTTTCAG GTGTTGTTGC AATTGCCTGC CCCACAATTG 
GAGGAAGTCC TAAGTTCGTG CCCAGATTTA CGTGAATCAT TATGCCTACA TGTTAAATCA 
TTTAATGAGG GACAGAAGGC AAATATTCCT CAAGCTGTGA TAGATGTCCT TGGTGTGGAT 
TTGTCTCAAG ATGATAAAAA ATCCGAAGAT GGCTCTATAA AAAATGACGA AGAAAACATT 
AAAGAACCAT TACCACCTGG ACAAGATCAT TTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015906087.1
CDS	157..3612
Translation	MDSTRKSSRHSSSSKEEYKRYSSSSRSSTRTETTEAQKSSLPSKRKAEETDLVQKLKKKTVECRRSTAAQFFVEEETVQESVTADRIVQLLNDAVTPKEENGKLKNLLIVKELIIQKEPKLLDNFLDEVVSFQHDTDPEVRRFIVQFIEDVCRRDLQFLPKVINNLLMMLHDESVKVQKKVIQTTTHLYPLTIRYFVNAKHVDDVMEAIWASMTELKLTIVSLLESDNEGVRTSATKFMEHVILAQTFKDQYADPKNTDLSLDEIPVILKVFRPRKMEEEAKDIFQRIVAFHCASHVSLCNLMACMQVLTSIGRQRFEFFPKVLEAFELLHANLPPTLGQSQVSSVRKNLKLELFQLVKHPGAVEFQSRISIMLSDLGASPQQISRCFPDAEAIKKPSVKIEDRSAKQEDIPSSSRPRKIKSSLEKGTKAKNTAIDITAEDLVPKFHSINVVDLVLVSMLSLPDVMPTNFQSTYTPIAAAGTEAQIKHLARLLATQLTLAGIGKGVEQMKHKMENEKVESDEDEAASNVPKPIQTLVSQTTEMKPKKQGLVLMPSGGSQIKANRISKKIDFQAIAKSMNEEESNNFCRAAYNRILNAEKMITKRGAAPIRTKLLTNLATRFRGDFSIDLKNHIMENPRNRLDLAFSWIYQEYSAYRGFECSIESHVENYEEAVDSLLGGILKSDEENHALYTRFFLDVPFITEGMAESLKTVCIDESSTAEALEMAESMSFRRPTKKLLFLGVICDLTLHENCDIRTKAVKAAVDIHESGHMRSFIEAFALRSLKFLLHTDPPTDFFLFFKQRPVTWSEDLTKVCLHLYLTLLPINHRLIQDLGTVYVEATGDIKRIILRALEAPVKGMSMASPELLLFVENCPKGAETLITRIIHILTDKAPPSAELVARVRDLYRKRVPDVRFFIPIINGLSKREIISALPELIQQKTPVVKEVLHRLLGSNFSSPISPAELLVALHNIDPSKCNLKIIIKATSLCLEEKNVYTQVVLAMVIQELMEQTPLPILLMRTVIQSLANYPHLLDFVTHILQRLILKEVWKQKKIWEGFIKCCQRTKPKSFQVLLQLPAPQLEEVLSSCPDLRESLCLHVKSFNEGQKANIPQAVIDVLGVDLSQDDKKSEDGSIKNDEENIKEPLPPGQDHL

Target ORF information:

RefSeq Version	XM_016050601.1
Organism	Parasteatoda tepidariorum(common house spider)
Definition	Parasteatoda tepidariorum symplekin (LOC107438324), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016050601.1

ORF Insert Sequence:

ATGGATTCAA CCAGGAAAAG TTCGAGACAT TCCTCATCTT CTAAGGAAGA GTATAAACGA 
TATTCTTCTT CAAGTAGAAG CTCTACTAGA ACGGAAACAA CTGAAGCTCA GAAAAGCTCT 
CTCCCCTCCA AAAGAAAAGC TGAAGAAACA GATTTGGTTC AAAAATTAAA AAAGAAAACT 
GTAGAATGTC GACGTAGCAC TGCTGCTCAA TTTTTTGTTG AAGAAGAAAC TGTACAAGAA 
TCAGTGACTG CTGATCGAAT TGTCCAGCTC CTCAATGATG CTGTTACACC AAAGGAAGAA 
AATGGAAAGC TGAAAAATCT GCTCATTGTC AAAGAACTGA TCATACAGAA AGAACCTAAG 
TTGCTGGACA ACTTTCTGGA TGAAGTAGTA AGTTTTCAAC ATGACACTGA TCCTGAAGTA 
CGCAGATTCA TTGTACAATT CATTGAGGAT GTTTGTAGGA GAGACTTACA ATTTCTTCCA 
AAAGTAATCA ACAATCTGTT GATGATGCTA CACGATGAAT CGGTCAAAGT TCAGAAAAAA 
GTTATCCAAA CAACAACTCA TCTTTATCCT CTAACCATTC GTTATTTTGT AAATGCAAAA 
CATGTTGATG ATGTAATGGA AGCTATTTGG GCTTCAATGA CAGAACTAAA ATTAACAATT 
GTCTCGCTCT TGGAGTCTGA CAATGAAGGA GTCCGTACTT CAGCTACAAA ATTTATGGAA 
CATGTTATTC TTGCACAAAC ATTCAAAGAC CAATATGCAG ATCCGAAAAA CACTGATTTA 
TCTTTGGATG AGATACCAGT GATATTAAAA GTTTTTCGAC CCAGGAAGAT GGAAGAAGAA 
GCCAAAGATA TTTTCCAGCG AATTGTTGCT TTTCATTGTG CTTCCCATGT GTCTCTTTGT 
AACCTTATGG CTTGCATGCA AGTTTTAACT TCAATAGGAA GGCAAAGATT TGAGTTTTTT 
CCTAAAGTTT TAGAAGCATT TGAATTGCTT CATGCAAATC TGCCACCAAC GTTGGGTCAA 
TCACAAGTGA GTAGTGTAAG AAAGAATTTA AAATTAGAGC TCTTTCAATT GGTTAAGCAT 
CCTGGCGCTG TCGAATTTCA ATCAAGAATA TCAATCATGC TCTCTGATTT AGGTGCATCA 
CCTCAGCAGA TAAGTAGATG CTTTCCGGAT GCAGAGGCAA TCAAGAAACC TAGCGTCAAA 
ATAGAGGACA GAAGTGCAAA ACAGGAAGAT ATACCATCTT CATCAAGACC TCGCAAAATA 
AAATCCAGTC TTGAGAAAGG AACTAAAGCT AAAAACACAG CTATTGATAT TACAGCTGAG 
GATTTAGTAC CCAAATTTCA CAGTATAAAT GTTGTTGATT TAGTATTAGT AAGCATGTTA 
AGTTTGCCTG ATGTTATGCC TACTAACTTT CAGTCCACAT ACACTCCTAT TGCAGCTGCT 
GGCACCGAAG CTCAGATTAA ACATCTGGCT CGTCTTTTAG CCACACAATT AACTTTGGCT 
GGAATTGGAA AAGGTGTGGA ACAAATGAAA CATAAAATGG AAAATGAAAA AGTGGAAAGT 
GATGAGGATG AGGCAGCCTC TAATGTACCC AAACCAATCC AAACTCTAGT AAGCCAAACT 
ACAGAAATGA AGCCTAAAAA GCAAGGGCTT GTTTTAATGC CTAGTGGTGG CTCCCAAATA 
AAAGCTAATA GAATATCTAA GAAAATCGAC TTCCAAGCCA TAGCGAAATC AATGAACGAA 
GAAGAGTCTA ATAATTTTTG TAGAGCTGCT TATAATCGAA TCTTGAATGC TGAAAAAATG 
ATTACTAAAA GAGGTGCTGC TCCAATTAGA ACAAAATTGC TTACTAACTT AGCTACCCGA 
TTTAGAGGTG ATTTTTCCAT TGATTTGAAA AATCATATCA TGGAAAATCC CCGCAATAGA 
TTAGATCTAG CATTTTCTTG GATATATCAA GAATATTCTG CTTATAGAGG ATTTGAATGT 
TCCATAGAAA GCCATGTTGA AAATTATGAA GAAGCAGTCG ATTCTCTATT GGGTGGCATT 
TTGAAGAGTG ACGAAGAAAA CCATGCTCTT TACACTCGTT TTTTCTTGGA TGTTCCATTT 
ATTACAGAAG GGATGGCTGA GTCTCTGAAA ACTGTTTGTA TTGATGAATC ATCTACTGCT 
GAAGCTCTTG AAATGGCTGA AAGCATGAGT TTTAGAAGAC CTACAAAGAA GTTGCTGTTT 
TTAGGAGTAA TATGTGACCT TACTCTTCAT GAAAATTGTG ACATACGGAC AAAAGCTGTA 
AAAGCAGCAG TTGACATCCA TGAAAGTGGC CATATGAGGT CATTCATTGA AGCTTTTGCT 
TTAAGAAGTC TCAAGTTTTT GTTACATACT GACCCACCAA CTGATTTCTT CCTGTTTTTC 
AAACAAAGGC CTGTTACTTG GTCTGAAGAT TTGACTAAAG TATGTTTACA TTTGTATTTA 
ACTTTGTTGC CCATAAACCA TCGCCTCATT CAAGATCTTG GAACTGTTTA TGTTGAAGCC 
ACTGGTGATA TTAAAAGAAT TATTCTCCGT GCCCTCGAAG CACCAGTTAA AGGCATGAGC 
ATGGCCTCTC CTGAACTCTT ACTCTTTGTT GAAAACTGTC CTAAAGGAGC CGAGACATTA 
ATTACAAGAA TCATCCATAT TTTGACTGAC AAAGCACCAC CATCAGCCGA ACTTGTTGCT 
AGAGTTCGAG ATTTATATCG TAAAAGAGTT CCAGACGTAC GTTTCTTTAT TCCAATCATT 
AATGGTCTTT CTAAGCGTGA AATAATTTCT GCATTACCTG AATTGATTCA ACAAAAAACA 
CCAGTAGTAA AAGAAGTTTT GCATCGTCTT TTAGGAAGTA ATTTTTCTAG CCCGATATCT 
CCAGCTGAAC TATTGGTCGC TCTGCATAAC ATTGATCCTT CTAAATGCAA CCTTAAAATA 
ATTATCAAAG CTACTTCACT CTGTTTGGAA GAGAAAAATG TTTACACTCA AGTTGTCTTG 
GCGATGGTTA TTCAGGAACT GATGGAACAA ACTCCTTTGC CTATTTTATT AATGAGAACT 
GTTATACAAT CTTTAGCAAA CTATCCTCAT CTTTTGGATT TTGTTACTCA CATATTGCAA 
CGGTTAATAT TGAAAGAAGT CTGGAAACAG AAGAAAATTT GGGAGGGTTT TATTAAGTGC 
TGCCAAAGAA CTAAACCAAA ATCTTTTCAG GTGTTGTTGC AATTGCCTGC CCCACAATTG 
GAGGAAGTCC TAAGTTCGTG CCCAGATTTA CGTGAATCAT TATGCCTACA TGTTAAATCA 
TTTAATGAGG GACAGAAGGC AAATATTCCT CAAGCTGTGA TAGATGTCCT TGGTGTGGAT 
TTGTCTCAAG ATGATAAAAA ATCCGAAGAT GGCTCTATAA AAAATGACGA AGAAAACATT 
AAAGAACCAT TACCACCTGG ACAAGATCAT TTGTAA