LOC114441227 cDNA ORF clone,

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gRNAs

The following LOC114441227 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC114441227 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OIn105326	XM_028414029.1 Latest version!	Parambassis ranga interleukin-6 receptor subunit beta-like (LOC114441227), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC114441227 ( XM_028414029.1 ) cDNA ORF clone, -> XP_028269830.1 Parambassis ranga interleukin-6 receptor subunit beta-like (LOC114441227), mRNA.

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CloneID	OIn105326
Clone ID Related Accession (Same CDS sequence)	XM_028414029.1
Accession Version	XM_028414029.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2232bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-13
Organism	Parambassis ranga(Indian glassy fish)
Product	interleukin-6 receptor subunit beta-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041030.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Parambassis ranga Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGACG TCTGGTGCCT CCAGCTGCAG CTCTGTGCCG TGAGCTGCCT CCTCCTGCAC 
GGCGGGGTTT CTCTCGAAGT GACGGTGAGA CACTCGGAGC TGCAGCAGTG TGAAGGAAAT 
AAACCTGTGT GTGTCACCGA CCCCAGAGAC TGCAGCCCAC GGCCGCCTTC ATCTGTTCAG 
GAGACCCTCA ACATGTCCTG TTACTACCAG CGGTCTGCAG ACCAGGTCCG GTCCATGACG 
TGTGCCTGGA GTCTGGAGTC TGACAGCCTG ACTGACGCCT CTCTGATCTT CAGCAGTAAA 
CGCGTCTATT CCTGTCGAGG CATCTTCAAC CCGGCAGCCG TCCTTAACGT GACAGCCCGG 
ATCAGGAACT ACCTGACGGG GACGGAGACC TGGTCTCAGC CTCACACTGT GGTTCTCCGT 
AAAGCAGTCA AGCCCCCTCA GCCCCTGCTG TCTGTGCTCA GCTCCACTGA GGACTCGGTA 
GTTGTGAGTT GGAGGAGCAG CGCTAATGGC AGCTGTCGGC TTCGCTACAG ACTCAGCGGC 
TCTCAGATGT GGATTCAGGC TGCAGATTCA GTCCCGGCAC ACAAAGATCA GGAGCTGACC 
CAGAGGATCC AAGGCCTCCA GCCATTCACA GTCTACAGCG CCGCCGTGGC CTGCAGGGGG 
GCGTCCAGCA TCTGGAGCGA CTGGAGCCTT GGCGTCAGCG GAAGGACACA GGACAGAGCG 
CCCTCCAGGC CTCCGCAGGT GTGTTACCGA GCCGAGAGAA CCGGGTCAGG TGATGTGCCT 
CTTCTTCATC TCATCTGGAA GACTTCTGAC CCTGTGGAGG CTGGAGCTCG TATCCTTGGA 
TACCAGGTAA GCTGCGAGTC CACTGGAGTG ACTCAAAACG TCACCGAGAC GACAGCGCTC 
CTGGTGGAGG AGGAGAAGAA CTGCAGCGTC GCTGTTCGCT CCTTCAACAC GGCGGGCTGC 
GGACCGGCTG CACGCCTCAC CATCGACCCA CAAAGGATGG ACGCTCCGCC CTCCGTCAGA 
AACTTGTGGG TTTCCAGCTT CTACCCGGGC AACAAATCCC TCCTGGTGCA GTGGGAGGCC 
TCCACCCTGC CTGTCAGCCA TTTTGTTGTC CAGTGGCGCT CTGAGACGAG TTCATCAACC 
CTCCGCTGGT CCTCAGTCAT CACCTCCATC ACCTCCGCTG TCATACAAGA TGTGGATCCT 
GATGAGTCCT ACCTGATCAG TGTGTTCCCC GTCCACCAGC AGCAGTGTGG ACCTCCTGAG 
TCTCTGCCTG CCAGTCTGCA GCACGGAGCT CTGATGGAGG CCGTCAGCCT GAACGTCATC 
CATGTGACTA AAACGACGGT GACCGCTGTG TGTGTGTGGC GGAGGACGTC TGGACCAATC 
AGAGTGAGCC GATACAGAGC GATGCTCAGG AGACACTCCG ATGGACGCGG TGGCCCTGCT 
CTGACTTTGT ACCCCGACCA ATCGCAGCAC ACCTTCCTCA ACCTGACACC CAACGCTCAG 
TATTCTCTCC TCCTATTGGC TGACAACATT TCTAGGAGCA TCGTCCCAGT GGTGACGTCT 
TTCGATGAGG TTCCTGTGGT TGCTACGGCG ACGCCTCTGC TGCTGCTGGC TGTCGTCGTG 
TTAATCATCG CCATCCTGTC CAGGACTGTG TACAAGTCGT ACTTCTTCCC GCCCATTTCC 
AGCCCTCGAG GAAGCACATC GGGCCGGTGG CTGATGGACC CGAACCTCGA GAAAGGTGCA 
GAGGGGACCA ACATCCTGGT TGTGGAGGAC CTCCAGGTGA CTGAAGTCCT CGTCAAGAAA 
AGCCTCATCA TGGTGGATCC AAAACCGCCA ATTGAACACG GAGACACGCC GTCGATCAGC 
CGCCTCCTCA TCCGGCTGGA TGCAGACTAC GTGTCCGCCG CCGCCTCGGA GCAGCAAGAG 
GCCTCTTCAC TGCAGGCCCG TCGGTCTGAT GATGAAGTCT TCATCCCCGA GATGGAGGAG 
AGCCGAGACG TCGCTCTGCT TCCTCACATA GAAGAAGAAG AAGACCGGCA GGGTGACGTC 
CCTCAGATGG CCAATCACAC TGAGCCGAGC GTGAGGTGTG GTGGTCAGGT GACCTGTGAG 
GCGGTATATG TGCGCTTCTC CTTTGTGGGA GAACGTGACG CTGAAACAGA ACGGACCCGT 
CTGATGTGTG AGCCGGAGTA TTTACCAAAC ACAGAGCCGA CCCGCTCTGA CCAGCTGACG 
GACAGCTGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028269830.1
CDS	175..2406
Translation	MMDVWCLQLQLCAVSCLLLHGGVSLEVTVRHSELQQCEGNKPVCVTDPRDCSPRPPSSVQETLNMSCYYQRSADQVRSMTCAWSLESDSLTDASLIFSSKRVYSCRGIFNPAAVLNVTARIRNYLTGTETWSQPHTVVLRKAVKPPQPLLSVLSSTEDSVVVSWRSSANGSCRLRYRLSGSQMWIQAADSVPAHKDQELTQRIQGLQPFTVYSAAVACRGASSIWSDWSLGVSGRTQDRAPSRPPQVCYRAERTGSGDVPLLHLIWKTSDPVEAGARILGYQVSCESTGVTQNVTETTALLVEEEKNCSVAVRSFNTAGCGPAARLTIDPQRMDAPPSVRNLWVSSFYPGNKSLLVQWEASTLPVSHFVVQWRSETSSSTLRWSSVITSITSAVIQDVDPDESYLISVFPVHQQQCGPPESLPASLQHGALMEAVSLNVIHVTKTTVTAVCVWRRTSGPIRVSRYRAMLRRHSDGRGGPALTLYPDQSQHTFLNLTPNAQYSLLLLADNISRSIVPVVTSFDEVPVVATATPLLLLAVVVLIIAILSRTVYKSYFFPPISSPRGSTSGRWLMDPNLEKGAEGTNILVVEDLQVTEVLVKKSLIMVDPKPPIEHGDTPSISRLLIRLDADYVSAAASEQQEASSLQARRSDDEVFIPEMEESRDVALLPHIEEEEDRQGDVPQMANHTEPSVRCGGQVTCEAVYVRFSFVGERDAETERTRLMCEPEYLPNTEPTRSDQLTDSC

Target ORF information:

RefSeq Version	XM_028414029.1
Organism	Parambassis ranga(Indian glassy fish)
Definition	Parambassis ranga interleukin-6 receptor subunit beta-like (LOC114441227), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_028414029.1

ORF Insert Sequence:

ATGATGGACG TCTGGTGCCT CCAGCTGCAG CTCTGTGCCG TGAGCTGCCT CCTCCTGCAC 
GGCGGGGTTT CTCTCGAAGT GACGGTGAGA CACTCGGAGC TGCAGCAGTG TGAAGGAAAT 
AAACCTGTGT GTGTCACCGA CCCCAGAGAC TGCAGCCCAC GGCCGCCTTC ATCTGTTCAG 
GAGACCCTCA ACATGTCCTG TTACTACCAG CGGTCTGCAG ACCAGGTCCG GTCCATGACG 
TGTGCCTGGA GTCTGGAGTC TGACAGCCTG ACTGACGCCT CTCTGATCTT CAGCAGTAAA 
CGCGTCTATT CCTGTCGAGG CATCTTCAAC CCGGCAGCCG TCCTTAACGT GACAGCCCGG 
ATCAGGAACT ACCTGACGGG GACGGAGACC TGGTCTCAGC CTCACACTGT GGTTCTCCGT 
AAAGCAGTCA AGCCCCCTCA GCCCCTGCTG TCTGTGCTCA GCTCCACTGA GGACTCGGTA 
GTTGTGAGTT GGAGGAGCAG CGCTAATGGC AGCTGTCGGC TTCGCTACAG ACTCAGCGGC 
TCTCAGATGT GGATTCAGGC TGCAGATTCA GTCCCGGCAC ACAAAGATCA GGAGCTGACC 
CAGAGGATCC AAGGCCTCCA GCCATTCACA GTCTACAGCG CCGCCGTGGC CTGCAGGGGG 
GCGTCCAGCA TCTGGAGCGA CTGGAGCCTT GGCGTCAGCG GAAGGACACA GGACAGAGCG 
CCCTCCAGGC CTCCGCAGGT GTGTTACCGA GCCGAGAGAA CCGGGTCAGG TGATGTGCCT 
CTTCTTCATC TCATCTGGAA GACTTCTGAC CCTGTGGAGG CTGGAGCTCG TATCCTTGGA 
TACCAGGTAA GCTGCGAGTC CACTGGAGTG ACTCAAAACG TCACCGAGAC GACAGCGCTC 
CTGGTGGAGG AGGAGAAGAA CTGCAGCGTC GCTGTTCGCT CCTTCAACAC GGCGGGCTGC 
GGACCGGCTG CACGCCTCAC CATCGACCCA CAAAGGATGG ACGCTCCGCC CTCCGTCAGA 
AACTTGTGGG TTTCCAGCTT CTACCCGGGC AACAAATCCC TCCTGGTGCA GTGGGAGGCC 
TCCACCCTGC CTGTCAGCCA TTTTGTTGTC CAGTGGCGCT CTGAGACGAG TTCATCAACC 
CTCCGCTGGT CCTCAGTCAT CACCTCCATC ACCTCCGCTG TCATACAAGA TGTGGATCCT 
GATGAGTCCT ACCTGATCAG TGTGTTCCCC GTCCACCAGC AGCAGTGTGG ACCTCCTGAG 
TCTCTGCCTG CCAGTCTGCA GCACGGAGCT CTGATGGAGG CCGTCAGCCT GAACGTCATC 
CATGTGACTA AAACGACGGT GACCGCTGTG TGTGTGTGGC GGAGGACGTC TGGACCAATC 
AGAGTGAGCC GATACAGAGC GATGCTCAGG AGACACTCCG ATGGACGCGG TGGCCCTGCT 
CTGACTTTGT ACCCCGACCA ATCGCAGCAC ACCTTCCTCA ACCTGACACC CAACGCTCAG 
TATTCTCTCC TCCTATTGGC TGACAACATT TCTAGGAGCA TCGTCCCAGT GGTGACGTCT 
TTCGATGAGG TTCCTGTGGT TGCTACGGCG ACGCCTCTGC TGCTGCTGGC TGTCGTCGTG 
TTAATCATCG CCATCCTGTC CAGGACTGTG TACAAGTCGT ACTTCTTCCC GCCCATTTCC 
AGCCCTCGAG GAAGCACATC GGGCCGGTGG CTGATGGACC CGAACCTCGA GAAAGGTGCA 
GAGGGGACCA ACATCCTGGT TGTGGAGGAC CTCCAGGTGA CTGAAGTCCT CGTCAAGAAA 
AGCCTCATCA TGGTGGATCC AAAACCGCCA ATTGAACACG GAGACACGCC GTCGATCAGC 
CGCCTCCTCA TCCGGCTGGA TGCAGACTAC GTGTCCGCCG CCGCCTCGGA GCAGCAAGAG 
GCCTCTTCAC TGCAGGCCCG TCGGTCTGAT GATGAAGTCT TCATCCCCGA GATGGAGGAG 
AGCCGAGACG TCGCTCTGCT TCCTCACATA GAAGAAGAAG AAGACCGGCA GGGTGACGTC 
CCTCAGATGG CCAATCACAC TGAGCCGAGC GTGAGGTGTG GTGGTCAGGT GACCTGTGAG 
GCGGTATATG TGCGCTTCTC CTTTGTGGGA GAACGTGACG CTGAAACAGA ACGGACCCGT 
CTGATGTGTG AGCCGGAGTA TTTACCAAAC ACAGAGCCGA CCCGCTCTGA CCAGCTGACG 
GACAGCTGTT AA