il6st cDNA ORF clone, Paralichthys olivaceus(Japanese flounder)

Gene
Clones
gRNAs

Summary

Gene Symbol	il6st
Entrez Gene ID	109643155
Full Name	interleukin 6 signal transducer
Gene Type	protein-coding
Organism	Paralichthys olivaceus(Japanese flounder)

mRNA and Protein(s)

mRNA	Protein	Name
XM_020108177.1	XP_019963736.1	interleukin-6 receptor subunit beta

Pathways from BioSystems
Gene Ontology

The following il6st gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the il6st cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPa256744	XM_020108177.1 Latest version!	Paralichthys olivaceus interleukin 6 signal transducer (il6st), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

il6st ( XM_020108177.1 ) cDNA ORF clone, Paralichthys olivaceus(Japanese flounder) -> XP_019963736.1 Paralichthys olivaceus interleukin 6 signal transducer (il6st), mRNA.

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CloneID	OPa256744
Clone ID Related Accession (Same CDS sequence)	XM_020108177.1
Accession Version	XM_020108177.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2460bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-07
Organism	Paralichthys olivaceus(Japanese flounder)
Product	interleukin-6 receptor subunit beta
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017860401.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Paralichthys olivaceus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCATCG ACACAGCTGA AGTCACAGCC GACGACCTCT ATTGGAATGT GTCTCAGGCG 
ACTGTACCCA AAGAACAGTA CACAAAGATC AACAGAACAG CTCTGAACGT CACCATTCGT 
GTCAGCGGTG AAGAAGTTGA ATGGTTATTT TGCCTCTGCA AGAAGGTGTC GGTCAACGTT 
GTCCTGAATC AAGGCAAATT TATGCACGGG ATCTTGCTTA GGAAAGGCTA TCGTCCGAGG 
AGGCCAGAGA ATCTTTCCTG CATCACTCTT CAGGAGGGAT CCTTATTCTC TCCAGTTATC 
ACGTGCAGCT GGGTCACAGG ACCTCAAACC AAAGTCTGCC CTACAAAGTT CACACTGTTT 
TTGAAAGTCA TCAATCATTC CGCTCACAAC GTGTCGACTG TAGAAAGCAG TGCCTCTGTG 
GCTATGTCCA TTTTCCCAAA CTACATGGAG CTGGAGATCT GGGTGGAGGC GAAGAACACA 
CTGGGAACCG TCGAGTCTGA GCGTCTCAAG AGAGAAGCCG AATATTTTGT GAAAACAAAT 
CCTCCTGCAA ACATCCAAAT CATCTCTGAG AAGAGTTTTC CCACCTCTCT TTTAATAACC 
TGGAGTCCTC CTATTGATAA ACATTATGTG ACATTAAAGT TTGAAATCAG ATTCTGCCAA 
AATGGATCTC ATGAGTGGAC GTATGTGCCT CATTCGGACA CTGCCATGGA TATTCAGTCC 
TTCAGACTCC AGAAACTGCT GCCAGACTCT GTTTATGTCA CTCAGGTTCG CTGCAAAAAC 
CTCAAAGACG ACCAGGGTTA CTGGAGTACA TGGAGCACCA ACGCTACCAA GAGAACGCCA 
GAGGACCGAC CGACAGGGAA ACCAGATTTA TGGAAATTCG TTGACAGTCA TGATGTTGGT 
AAACGACTAT TGCAGTTCAT CTGTAAGGAT CCTGTGTTAA CTAACGGGAG GATAACAAGA 
TTCAAAATGA AGATTGGAAG TCAGGAGTGG ATTATCCCCG TCAACCACTC AGAGGCTGAC 
GGCCGCTCCA ACCACAGGCG CATCACTTCC CTCAAAAAGG TGCACCTGTC CGACAAGAAA 
TCTCTCGTGT ACGTCACTGC CGTCAACTCA GTGGGGGAAT CTGCTCCAGC GTTGATTGTC 
ATCCCTGAAA AAGGACGTGA GCTTGGCCCA GTGCAGCAGC TGAGGGTGGG GGTCCAAGGA 
GGCCAGATGT GGCTGCAGTG GACCCCCCCG AACACTGGAA CTGTGAAGGA GTACGTGGTG 
CAGTGGCTCA CTGGAGATGT GTTAGACTGG CAGAGAGAAA ACAGAAGCAC CAGACACACT 
GTTATTAAAG GCAACCTGGA GAAGTTTGTC TGCTACAACG TGTCTGTGTA TCCGATATAT 
TCTGGAGGGA CGGGGCAGGC AGCGACTGTC CAAGCCTTTC TGGAACAAAG CGTTCCACTG 
TCTGGCCCCA ATGTGGAACT GACCAGTAAA GCGGGACATA CAGAGGCTGA GCTTCAGTGG 
AAAGAGATTC CCCAAAACCA TCGACGAGGC TTCATCACAA ACTACACAAT ATTCTACCAA 
AGTGGGGATA AATTACACTC GGTGACGGTT CCGTCAAACA CCACCTCATT CACTTTGACA 
GGACTGTCAG GAAACACCAG GTACAACACA TGGATCAGTG CCTCAACCAT CGCAGGTTCA 
ACCAAAGGCA ACAATCACTC GTTCACCACT CTGAGATACG CAACGGGAGA GATTGAGTTC 
ATCATGGCCG GAGCGAGCCT TGGCTTTCTG TTTGTTATCG TCATGACGAT GGTGCTCTGT 
ATTTCCAAAA AAGACATCAT CAAGAGGAAC TTCTGGCCTC AGATTCCAAA CCCTGGAGAG 
AGCACGATTG GAAACTGGTC TCCTGATCAA CCGTCAAAGG CAGAGACGCC AAAGCAGAAC 
TGTCTGTCTG GCATCAGTGT CCTCGACATG GACATGTGCG ATGGAAAGAT GGTGTTTGAT 
GAAGACAAAA CCAGTCTCTC TCTGAAGAAG GACAAGTATC TGTCCGAGGA GCACAGCAGT 
GGCATCGGTG GCTCCTCCTG CATGTCTTCG CCTCGCCAGA GCGTTTCCGA CAGCGATGAG 
GGCGGAGACT TGGCGGACAC CACGGCCAGT ACCGTGCAGT ACTCCTCGGT GGTGGCCTCC 
AACGGTTACA AGGGTCAGAC CCCGAGCTCG CAGCCACAGT CGGCCTTCTT CTCCCGCTCC 
GAGTCCACGC AGCCCCTGCT GGATTGCGAG GAGAACCCGG ACATATTGGT GCAGGACGGC 
AGCAGACAGT CCCAGCGCTT CCTGCGACGG CCTGCGGAGG AGGCGCTAGA GCCTAAGGAC 
TTCTGCCCCC TGGAGGAGGA CTCTGAGCTG ACACTAACAG ATGAGCAGTC AGGTGATTGG 
CTGCCGACAG CGGCAGCCTC CAGCTACATG CCTCAGCTGG GAGGCTACAG GCAGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019963736.1
CDS	75..2534
Translation	MIIDTAEVTADDLYWNVSQATVPKEQYTKINRTALNVTIRVSGEEVEWLFCLCKKVSVNVVLNQGKFMHGILLRKGYRPRRPENLSCITLQEGSLFSPVITCSWVTGPQTKVCPTKFTLFLKVINHSAHNVSTVESSASVAMSIFPNYMELEIWVEAKNTLGTVESERLKREAEYFVKTNPPANIQIISEKSFPTSLLITWSPPIDKHYVTLKFEIRFCQNGSHEWTYVPHSDTAMDIQSFRLQKLLPDSVYVTQVRCKNLKDDQGYWSTWSTNATKRTPEDRPTGKPDLWKFVDSHDVGKRLLQFICKDPVLTNGRITRFKMKIGSQEWIIPVNHSEADGRSNHRRITSLKKVHLSDKKSLVYVTAVNSVGESAPALIVIPEKGRELGPVQQLRVGVQGGQMWLQWTPPNTGTVKEYVVQWLTGDVLDWQRENRSTRHTVIKGNLEKFVCYNVSVYPIYSGGTGQAATVQAFLEQSVPLSGPNVELTSKAGHTEAELQWKEIPQNHRRGFITNYTIFYQSGDKLHSVTVPSNTTSFTLTGLSGNTRYNTWISASTIAGSTKGNNHSFTTLRYATGEIEFIMAGASLGFLFVIVMTMVLCISKKDIIKRNFWPQIPNPGESTIGNWSPDQPSKAETPKQNCLSGISVLDMDMCDGKMVFDEDKTSLSLKKDKYLSEEHSSGIGGSSCMSSPRQSVSDSDEGGDLADTTASTVQYSSVVASNGYKGQTPSSQPQSAFFSRSESTQPLLDCEENPDILVQDGSRQSQRFLRRPAEEALEPKDFCPLEEDSELTLTDEQSGDWLPTAAASSYMPQLGGYRQQ

Target ORF information:

RefSeq Version	XM_020108177.1
Organism	Paralichthys olivaceus(Japanese flounder)
Definition	Paralichthys olivaceus interleukin 6 signal transducer (il6st), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020108177.1

ORF Insert Sequence:

ATGATCATCG ACACAGCTGA AGTCACAGCC GACGACCTCT ATTGGAATGT GTCTCAGGCG 
ACTGTACCCA AAGAACAGTA CACAAAGATC AACAGAACAG CTCTGAACGT CACCATTCGT 
GTCAGCGGTG AAGAAGTTGA ATGGTTATTT TGCCTCTGCA AGAAGGTGTC GGTCAACGTT 
GTCCTGAATC AAGGCAAATT TATGCACGGG ATCTTGCTTA GGAAAGGCTA TCGTCCGAGG 
AGGCCAGAGA ATCTTTCCTG CATCACTCTT CAGGAGGGAT CCTTATTCTC TCCAGTTATC 
ACGTGCAGCT GGGTCACAGG ACCTCAAACC AAAGTCTGCC CTACAAAGTT CACACTGTTT 
TTGAAAGTCA TCAATCATTC CGCTCACAAC GTGTCGACTG TAGAAAGCAG TGCCTCTGTG 
GCTATGTCCA TTTTCCCAAA CTACATGGAG CTGGAGATCT GGGTGGAGGC GAAGAACACA 
CTGGGAACCG TCGAGTCTGA GCGTCTCAAG AGAGAAGCCG AATATTTTGT GAAAACAAAT 
CCTCCTGCAA ACATCCAAAT CATCTCTGAG AAGAGTTTTC CCACCTCTCT TTTAATAACC 
TGGAGTCCTC CTATTGATAA ACATTATGTG ACATTAAAGT TTGAAATCAG ATTCTGCCAA 
AATGGATCTC ATGAGTGGAC GTATGTGCCT CATTCGGACA CTGCCATGGA TATTCAGTCC 
TTCAGACTCC AGAAACTGCT GCCAGACTCT GTTTATGTCA CTCAGGTTCG CTGCAAAAAC 
CTCAAAGACG ACCAGGGTTA CTGGAGTACA TGGAGCACCA ACGCTACCAA GAGAACGCCA 
GAGGACCGAC CGACAGGGAA ACCAGATTTA TGGAAATTCG TTGACAGTCA TGATGTTGGT 
AAACGACTAT TGCAGTTCAT CTGTAAGGAT CCTGTGTTAA CTAACGGGAG GATAACAAGA 
TTCAAAATGA AGATTGGAAG TCAGGAGTGG ATTATCCCCG TCAACCACTC AGAGGCTGAC 
GGCCGCTCCA ACCACAGGCG CATCACTTCC CTCAAAAAGG TGCACCTGTC CGACAAGAAA 
TCTCTCGTGT ACGTCACTGC CGTCAACTCA GTGGGGGAAT CTGCTCCAGC GTTGATTGTC 
ATCCCTGAAA AAGGACGTGA GCTTGGCCCA GTGCAGCAGC TGAGGGTGGG GGTCCAAGGA 
GGCCAGATGT GGCTGCAGTG GACCCCCCCG AACACTGGAA CTGTGAAGGA GTACGTGGTG 
CAGTGGCTCA CTGGAGATGT GTTAGACTGG CAGAGAGAAA ACAGAAGCAC CAGACACACT 
GTTATTAAAG GCAACCTGGA GAAGTTTGTC TGCTACAACG TGTCTGTGTA TCCGATATAT 
TCTGGAGGGA CGGGGCAGGC AGCGACTGTC CAAGCCTTTC TGGAACAAAG CGTTCCACTG 
TCTGGCCCCA ATGTGGAACT GACCAGTAAA GCGGGACATA CAGAGGCTGA GCTTCAGTGG 
AAAGAGATTC CCCAAAACCA TCGACGAGGC TTCATCACAA ACTACACAAT ATTCTACCAA 
AGTGGGGATA AATTACACTC GGTGACGGTT CCGTCAAACA CCACCTCATT CACTTTGACA 
GGACTGTCAG GAAACACCAG GTACAACACA TGGATCAGTG CCTCAACCAT CGCAGGTTCA 
ACCAAAGGCA ACAATCACTC GTTCACCACT CTGAGATACG CAACGGGAGA GATTGAGTTC 
ATCATGGCCG GAGCGAGCCT TGGCTTTCTG TTTGTTATCG TCATGACGAT GGTGCTCTGT 
ATTTCCAAAA AAGACATCAT CAAGAGGAAC TTCTGGCCTC AGATTCCAAA CCCTGGAGAG 
AGCACGATTG GAAACTGGTC TCCTGATCAA CCGTCAAAGG CAGAGACGCC AAAGCAGAAC 
TGTCTGTCTG GCATCAGTGT CCTCGACATG GACATGTGCG ATGGAAAGAT GGTGTTTGAT 
GAAGACAAAA CCAGTCTCTC TCTGAAGAAG GACAAGTATC TGTCCGAGGA GCACAGCAGT 
GGCATCGGTG GCTCCTCCTG CATGTCTTCG CCTCGCCAGA GCGTTTCCGA CAGCGATGAG 
GGCGGAGACT TGGCGGACAC CACGGCCAGT ACCGTGCAGT ACTCCTCGGT GGTGGCCTCC 
AACGGTTACA AGGGTCAGAC CCCGAGCTCG CAGCCACAGT CGGCCTTCTT CTCCCGCTCC 
GAGTCCACGC AGCCCCTGCT GGATTGCGAG GAGAACCCGG ACATATTGGT GCAGGACGGC 
AGCAGACAGT CCCAGCGCTT CCTGCGACGG CCTGCGGAGG AGGCGCTAGA GCCTAAGGAC 
TTCTGCCCCC TGGAGGAGGA CTCTGAGCTG ACACTAACAG ATGAGCAGTC AGGTGATTGG 
CTGCCGACAG CGGCAGCCTC CAGCTACATG CCTCAGCTGG GAGGCTACAG GCAGCAGTGA