bmp2k cDNA ORF clone, Paralichthys olivaceus(Japanese flounder)

Gene
Clones
gRNAs

Summary

Gene Symbol	bmp2k
Entrez Gene ID	109641322
Full Name	BMP2 inducible kinase
Gene Type	protein-coding
Organism	Paralichthys olivaceus(Japanese flounder)

mRNA and Protein(s)

mRNA	Protein	Name
XM_020105756.1	XP_019961315.1	BMP-2-inducible protein kinase isoform X2
XM_020105755.1	XP_019961314.1	BMP-2-inducible protein kinase isoform X1

Pathways from BioSystems
Gene Ontology

The following bmp2k gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the bmp2k cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPa255058	XM_020105755.1 Latest version!	Paralichthys olivaceus BMP2 inducible kinase (bmp2k), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OPa255059	XM_020105756.1 Latest version!	Paralichthys olivaceus BMP2 inducible kinase (bmp2k), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

bmp2k ( XM_020105755.1 ) cDNA ORF clone, Paralichthys olivaceus(Japanese flounder) -> XP_019961314.1 Paralichthys olivaceus BMP2 inducible kinase (bmp2k), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPa255058
Clone ID Related Accession (Same CDS sequence)	XM_020105755.1
Accession Version	XM_020105755.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3489bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-07
Organism	Paralichthys olivaceus(Japanese flounder)
Product	BMP-2-inducible protein kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017859835.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Paralichthys olivaceus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAGT TTTCCAGGAT GCCAAAGTCG GAAAGCGGCG GGCTCGGAGG GGCCGGGTCC 
GGCTGCAGCT CCGGAGTCAG CAGCTACTTC GGGAAAGTGT TCGCAGTGGG TCGGTACCAG 
GTCACCGTGG AGGAGCTGGT CGCTGAAGGA GGCTTCTCCG TTGTGTTTCT GGCTCGTACG 
CACAGCGGCG TCCGCTGCGC TCTGAAACGG ATGTACGTCA ACAACGTCCC GGATCTGAAT 
GTCTACAAGA GAGAGATCAC TATCATGAAG GAGCTCTCAG GCCACAAGAA CATCGTCGGC 
TACCTGGACT CTACAATCAG TGCCGTGTCC GACAGCGTCT GGGAGGTCCT GATCCTCATG 
GAGTACTGCA AAGCGGGTCA GGTGGTGAAG CAGATGAACC AGCGGTTGAA TGTGGGCTTC 
AGTGAGGCCG AGGTCCTTCA CATCTTCTGT GACACCTGTG AGGCCGTGGC CCGGCTGCAC 
CAATGCAAGA CGCCCGTCAT CCACAGAGAC CTGAAGGTTG AAAACATCCT GCTGAACGAC 
CAGGGGAACT ACGTGCTGTG TGACTTTGGA AGCGCGACTC ACAAGATTTT GCTGCCACAT 
AAAGATGGAG TCACTGCTGT GGAGGACGAG ATTAAAAAGT ACACGACCCT GTCATATCGG 
GCGCCGGAGA TGATTAACTT GTATGCAGGG ATAGCCATCA CCACTAAAGC TGATATATGG 
GCACTCGGAT GCTTGTTGTA CAAGCTGTGT TTCTTCGCTC TTCCATTTGG GGAGAGTCAA 
GTTGCCATAT GTGACGGAAC CTTTATCGTT CCAGATAACT CCAAATTCTC CTTCAAGTTG 
CACTGCTTAA TCAGATATAT GCTCGAACCA GATCAGGAGA AGAGACCAGA CATTTACCAA 
GTGTCCTACT TTGCCTTCAA GTTAGCTGGA AAAGACTGTC CGGTGCCAAA TCTCTTTAAC 
TCTCCGATCC CCACGTCGCT CCCAGAGCCT CTAACGGCCA GCGAGGTCGC TGCTAAGAAA 
AGCCAGACAA AAGCCAGGAT AACGGAATCT GTCGGCCCAA CGGAAACATC AATAGCTCCC 
AGGCAGAGGC CGAAGGCGGT GAACAGTAAC GTTCTGCCCC TCGCCAGCAC CGTCACCCCC 
GTGAAGATGA CCATGCCTTC AGCCCCCGTC AGCAATGGAC AGAAAGCTCC CACCCCTGTC 
TCCGGGCAGC TGTCCATGCA GCCCCAGCCC AGCAGTCAGC AGCACCGAGT CCTGCAGCAG 
CTGCAGCCCG GTGACCTGCG TCTGCAGCAA CTTCAGCAGC ATCAACAACA ACAACAACAA 
CAAGCCGTGC AACAGCAACA TGTTAATGCA CAGCAACTCC AATACCTCCA GTACCAACAG 
GCTGTGCAGC AGTCCCTCCA GCTCCAGCAG CAGCAGGCTC TGCTCCAGCA GCAGCAGCAG 
CAGCAGCAGA TGATGATGCA GCCGATGTAC CAGCAGCAGG TCACTCAGGC CCAGGCTCAG 
TATGCAGCCA TGTTGCACCA GTACCAACAG GCTTTTGTGC AGCAGCAGCA GCAGCATCAC 
CACCAACAAC AACATCATCA TCTACAGCAG CAGCAGCAGC TTCATCCCGC CCAGCAGCCT 
CTCCCCTACA TGTCCTCCCC TCTTGAGTTC CAGATCCCCC TCGGCTCCTA TAACACTACC 
ACCCCCTCTG CTGGACCTGC AGCAGGAAGT GGACAGTTGG GGGGAATATC ACCTGTGGAT 
CCCTTGTACA CGAACTCCAG CAGAAACCCT CTTCTGGCCT CTGATGGCAC CACCCCTCCC 
CCTCAGAGCT GCAACAGCAC AGTGAGTAAC CCCCCCGACA TGTCACGCTG GAACCCTTTC 
GGAGAGGACA ACTTCTCCAA GCTGACGGAG GAGGAGCTGA TCGACCGGGA GTTCGACATG 
CTCCGAGCAA ACAAACCTGT GGAGAGAACA GCCTGCGTGG AAGCGGACCG ACCGCAGCCC 
GCTGCCATCA AGCCCCTCCC ACCCGAGGAC CTGTTCGGCT CAGTCCCGTT TGTGGCCAGC 
GCAGGATCTA CCACCGTGAC GCAGACTGAG CAGAGCGGCG AGGACGTCAG CGTTCCTCCT 
CCCGTCTCGA CCTCCGCAGA CGATCTGCAG GCGCCCGCGG TCAAGGAGCA CAAACCCGGC 
AGGAAGAGTA ACTGCAGATC GGGAGAAGAG TCCGACAGCG ACTTCGAGTC TGACCCTCCT 
TCACCAAAAA GCAGCGAGGA AGAGGAGCCG GAGGAGGACG AGGCTCTGAA CAGTGAACAT 
GGTGAAGACA CAGAGCCAGA GAATATAGGA CAGAGGCCTC TGCTGATGGA TTCTGAGGAA 
GAGGAGGAGG AGGACAAGCA CAGTTCTGAC TCGGACTACA ACCCCAGCAG AGTCAAGGGT 
CGCTCGAAGA AGTCTGGTAA ATCCGCTTCT GCTGCCACCA GGAGGAGCCC TGAAGGAGAT 
TCTGCCATGA CCCTGATCAC CCCTCCCGAA ACGCCCAGCG GGGCGAGAGC CGCCCCAGCT 
GAGGAAGCTG TGGATGTGTT TGGGTCCATT CCCTTCGTCA GAGGAGCTTC ACCGGTTGAG 
CCCTCGGAGA ACGCGGACAT CTTTGCCAAA GCGCCTTTCA GACAAGTCAG CCAAGAGCCG 
CGAGCAGCCG ATGAGTTTGA TGTTTTTACC AAAGCTCCGT TCAACCGGAA CCTCTCCAAG 
TCTGGCAAGA GCGGCAGCGA AGCTCCCGCG GGCCAATCGC CGCCCACCTC CCCCGAGGCC 
TTTGACATTT TTGGCTTCTC TCCCTTCCAC CCGGGCCCCA CGGCGCCACC TCCCGTGACC 
TCGAGGAGCG CGGACGATAT CTTTCAGTCG TCTTACGAGG AGTCGGCGAG TCCTCAGCAA 
CAGAGGCTGA AGCAGCGCAG CCTCCAGAAG CTGTCGTCGC GGCAGCGGAA AACCAAGCAA 
GAGGCCACCG CGGGGGGCAG CAACGGCAAA CGACACCACG GTACACCCAC CGGGGGGCGC 
AAGACTGGCA AGCCAACTTA CCGCACCCCT GAGCGAGTCC GCAGGCACAA GAAGGTCGGC 
CGGAGAGACT CTCAGAGCAG CAACGAGTTC CTCAGCACCT CCGACTCTAA AGAGAACATC 
AGCGTTGATA TAACCATGGC TGAGGGGAAG GACAAGGGAG CTTCTCTGCC GGTGGACGAG 
GCCCTTTTAG ACCCGTTTGG AGCCAAACCT TTCCACCCTC CGGACGGAAG CCGGCACAGC 
CAGTACCAAG GCCTCGTGGA TAGTAAGAGT GACATGGGCA CGGCCAACGG TAAGACCTGG 
ACGGGCTCTC TTCACGGAGC TCTTGGAGAG AGTAGAGATA TGGATGACTT CGGGGCAGTG 
CCCTTCACAG AACTGGTCGT CCACAGCGGA CCCCAGCAGC AGCCGCCGCC GCAGCAGCAG 
CAGCAGGCGC CCTCGCAGCC GGGGGAGCTG GACCCGTTCG GAGCTGCACC TTTTCCCTCG 
AAACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019961314.1
CDS	907..4395
Translation	MKKFSRMPKSESGGLGGAGSGCSSGVSSYFGKVFAVGRYQVTVEELVAEGGFSVVFLARTHSGVRCALKRMYVNNVPDLNVYKREITIMKELSGHKNIVGYLDSTISAVSDSVWEVLILMEYCKAGQVVKQMNQRLNVGFSEAEVLHIFCDTCEAVARLHQCKTPVIHRDLKVENILLNDQGNYVLCDFGSATHKILLPHKDGVTAVEDEIKKYTTLSYRAPEMINLYAGIAITTKADIWALGCLLYKLCFFALPFGESQVAICDGTFIVPDNSKFSFKLHCLIRYMLEPDQEKRPDIYQVSYFAFKLAGKDCPVPNLFNSPIPTSLPEPLTASEVAAKKSQTKARITESVGPTETSIAPRQRPKAVNSNVLPLASTVTPVKMTMPSAPVSNGQKAPTPVSGQLSMQPQPSSQQHRVLQQLQPGDLRLQQLQQHQQQQQQQAVQQQHVNAQQLQYLQYQQAVQQSLQLQQQQALLQQQQQQQQMMMQPMYQQQVTQAQAQYAAMLHQYQQAFVQQQQQHHHQQQHHHLQQQQQLHPAQQPLPYMSSPLEFQIPLGSYNTTTPSAGPAAGSGQLGGISPVDPLYTNSSRNPLLASDGTTPPPQSCNSTVSNPPDMSRWNPFGEDNFSKLTEEELIDREFDMLRANKPVERTACVEADRPQPAAIKPLPPEDLFGSVPFVASAGSTTVTQTEQSGEDVSVPPPVSTSADDLQAPAVKEHKPGRKSNCRSGEESDSDFESDPPSPKSSEEEEPEEDEALNSEHGEDTEPENIGQRPLLMDSEEEEEEDKHSSDSDYNPSRVKGRSKKSGKSASAATRRSPEGDSAMTLITPPETPSGARAAPAEEAVDVFGSIPFVRGASPVEPSENADIFAKAPFRQVSQEPRAADEFDVFTKAPFNRNLSKSGKSGSEAPAGQSPPTSPEAFDIFGFSPFHPGPTAPPPVTSRSADDIFQSSYEESASPQQQRLKQRSLQKLSSRQRKTKQEATAGGSNGKRHHGTPTGGRKTGKPTYRTPERVRRHKKVGRRDSQSSNEFLSTSDSKENISVDITMAEGKDKGASLPVDEALLDPFGAKPFHPPDGSRHSQYQGLVDSKSDMGTANGKTWTGSLHGALGESRDMDDFGAVPFTELVVHSGPQQQPPPQQQQQAPSQPGELDPFGAAPFPSKQ

Target ORF information:

RefSeq Version	XM_020105755.1
Organism	Paralichthys olivaceus(Japanese flounder)
Definition	Paralichthys olivaceus BMP2 inducible kinase (bmp2k), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020105755.1

ORF Insert Sequence:

ATGAAGAAGT TTTCCAGGAT GCCAAAGTCG GAAAGCGGCG GGCTCGGAGG GGCCGGGTCC 
GGCTGCAGCT CCGGAGTCAG CAGCTACTTC GGGAAAGTGT TCGCAGTGGG TCGGTACCAG 
GTCACCGTGG AGGAGCTGGT CGCTGAAGGA GGCTTCTCCG TTGTGTTTCT GGCTCGTACG 
CACAGCGGCG TCCGCTGCGC TCTGAAACGG ATGTACGTCA ACAACGTCCC GGATCTGAAT 
GTCTACAAGA GAGAGATCAC TATCATGAAG GAGCTCTCAG GCCACAAGAA CATCGTCGGC 
TACCTGGACT CTACAATCAG TGCCGTGTCC GACAGCGTCT GGGAGGTCCT GATCCTCATG 
GAGTACTGCA AAGCGGGTCA GGTGGTGAAG CAGATGAACC AGCGGTTGAA TGTGGGCTTC 
AGTGAGGCCG AGGTCCTTCA CATCTTCTGT GACACCTGTG AGGCCGTGGC CCGGCTGCAC 
CAATGCAAGA CGCCCGTCAT CCACAGAGAC CTGAAGGTTG AAAACATCCT GCTGAACGAC 
CAGGGGAACT ACGTGCTGTG TGACTTTGGA AGCGCGACTC ACAAGATTTT GCTGCCACAT 
AAAGATGGAG TCACTGCTGT GGAGGACGAG ATTAAAAAGT ACACGACCCT GTCATATCGG 
GCGCCGGAGA TGATTAACTT GTATGCAGGG ATAGCCATCA CCACTAAAGC TGATATATGG 
GCACTCGGAT GCTTGTTGTA CAAGCTGTGT TTCTTCGCTC TTCCATTTGG GGAGAGTCAA 
GTTGCCATAT GTGACGGAAC CTTTATCGTT CCAGATAACT CCAAATTCTC CTTCAAGTTG 
CACTGCTTAA TCAGATATAT GCTCGAACCA GATCAGGAGA AGAGACCAGA CATTTACCAA 
GTGTCCTACT TTGCCTTCAA GTTAGCTGGA AAAGACTGTC CGGTGCCAAA TCTCTTTAAC 
TCTCCGATCC CCACGTCGCT CCCAGAGCCT CTAACGGCCA GCGAGGTCGC TGCTAAGAAA 
AGCCAGACAA AAGCCAGGAT AACGGAATCT GTCGGCCCAA CGGAAACATC AATAGCTCCC 
AGGCAGAGGC CGAAGGCGGT GAACAGTAAC GTTCTGCCCC TCGCCAGCAC CGTCACCCCC 
GTGAAGATGA CCATGCCTTC AGCCCCCGTC AGCAATGGAC AGAAAGCTCC CACCCCTGTC 
TCCGGGCAGC TGTCCATGCA GCCCCAGCCC AGCAGTCAGC AGCACCGAGT CCTGCAGCAG 
CTGCAGCCCG GTGACCTGCG TCTGCAGCAA CTTCAGCAGC ATCAACAACA ACAACAACAA 
CAAGCCGTGC AACAGCAACA TGTTAATGCA CAGCAACTCC AATACCTCCA GTACCAACAG 
GCTGTGCAGC AGTCCCTCCA GCTCCAGCAG CAGCAGGCTC TGCTCCAGCA GCAGCAGCAG 
CAGCAGCAGA TGATGATGCA GCCGATGTAC CAGCAGCAGG TCACTCAGGC CCAGGCTCAG 
TATGCAGCCA TGTTGCACCA GTACCAACAG GCTTTTGTGC AGCAGCAGCA GCAGCATCAC 
CACCAACAAC AACATCATCA TCTACAGCAG CAGCAGCAGC TTCATCCCGC CCAGCAGCCT 
CTCCCCTACA TGTCCTCCCC TCTTGAGTTC CAGATCCCCC TCGGCTCCTA TAACACTACC 
ACCCCCTCTG CTGGACCTGC AGCAGGAAGT GGACAGTTGG GGGGAATATC ACCTGTGGAT 
CCCTTGTACA CGAACTCCAG CAGAAACCCT CTTCTGGCCT CTGATGGCAC CACCCCTCCC 
CCTCAGAGCT GCAACAGCAC AGTGAGTAAC CCCCCCGACA TGTCACGCTG GAACCCTTTC 
GGAGAGGACA ACTTCTCCAA GCTGACGGAG GAGGAGCTGA TCGACCGGGA GTTCGACATG 
CTCCGAGCAA ACAAACCTGT GGAGAGAACA GCCTGCGTGG AAGCGGACCG ACCGCAGCCC 
GCTGCCATCA AGCCCCTCCC ACCCGAGGAC CTGTTCGGCT CAGTCCCGTT TGTGGCCAGC 
GCAGGATCTA CCACCGTGAC GCAGACTGAG CAGAGCGGCG AGGACGTCAG CGTTCCTCCT 
CCCGTCTCGA CCTCCGCAGA CGATCTGCAG GCGCCCGCGG TCAAGGAGCA CAAACCCGGC 
AGGAAGAGTA ACTGCAGATC GGGAGAAGAG TCCGACAGCG ACTTCGAGTC TGACCCTCCT 
TCACCAAAAA GCAGCGAGGA AGAGGAGCCG GAGGAGGACG AGGCTCTGAA CAGTGAACAT 
GGTGAAGACA CAGAGCCAGA GAATATAGGA CAGAGGCCTC TGCTGATGGA TTCTGAGGAA 
GAGGAGGAGG AGGACAAGCA CAGTTCTGAC TCGGACTACA ACCCCAGCAG AGTCAAGGGT 
CGCTCGAAGA AGTCTGGTAA ATCCGCTTCT GCTGCCACCA GGAGGAGCCC TGAAGGAGAT 
TCTGCCATGA CCCTGATCAC CCCTCCCGAA ACGCCCAGCG GGGCGAGAGC CGCCCCAGCT 
GAGGAAGCTG TGGATGTGTT TGGGTCCATT CCCTTCGTCA GAGGAGCTTC ACCGGTTGAG 
CCCTCGGAGA ACGCGGACAT CTTTGCCAAA GCGCCTTTCA GACAAGTCAG CCAAGAGCCG 
CGAGCAGCCG ATGAGTTTGA TGTTTTTACC AAAGCTCCGT TCAACCGGAA CCTCTCCAAG 
TCTGGCAAGA GCGGCAGCGA AGCTCCCGCG GGCCAATCGC CGCCCACCTC CCCCGAGGCC 
TTTGACATTT TTGGCTTCTC TCCCTTCCAC CCGGGCCCCA CGGCGCCACC TCCCGTGACC 
TCGAGGAGCG CGGACGATAT CTTTCAGTCG TCTTACGAGG AGTCGGCGAG TCCTCAGCAA 
CAGAGGCTGA AGCAGCGCAG CCTCCAGAAG CTGTCGTCGC GGCAGCGGAA AACCAAGCAA 
GAGGCCACCG CGGGGGGCAG CAACGGCAAA CGACACCACG GTACACCCAC CGGGGGGCGC 
AAGACTGGCA AGCCAACTTA CCGCACCCCT GAGCGAGTCC GCAGGCACAA GAAGGTCGGC 
CGGAGAGACT CTCAGAGCAG CAACGAGTTC CTCAGCACCT CCGACTCTAA AGAGAACATC 
AGCGTTGATA TAACCATGGC TGAGGGGAAG GACAAGGGAG CTTCTCTGCC GGTGGACGAG 
GCCCTTTTAG ACCCGTTTGG AGCCAAACCT TTCCACCCTC CGGACGGAAG CCGGCACAGC 
CAGTACCAAG GCCTCGTGGA TAGTAAGAGT GACATGGGCA CGGCCAACGG TAAGACCTGG 
ACGGGCTCTC TTCACGGAGC TCTTGGAGAG AGTAGAGATA TGGATGACTT CGGGGCAGTG 
CCCTTCACAG AACTGGTCGT CCACAGCGGA CCCCAGCAGC AGCCGCCGCC GCAGCAGCAG 
CAGCAGGCGC CCTCGCAGCC GGGGGAGCTG GACCCGTTCG GAGCTGCACC TTTTCCCTCG 
AAACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bmp2k ( XM_020105756.1 ) cDNA ORF clone, Paralichthys olivaceus(Japanese flounder) -> XP_019961315.1 Paralichthys olivaceus BMP2 inducible kinase (bmp2k), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPa255059
Clone ID Related Accession (Same CDS sequence)	XM_020105756.1
Accession Version	XM_020105756.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3486bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-07
Organism	Paralichthys olivaceus(Japanese flounder)
Product	BMP-2-inducible protein kinase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017859835.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Paralichthys olivaceus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAGT TTTCCAGGAT GCCAAAGTCG GAAAGCGGCG GGCTCGGAGG GGCCGGGTCC 
GGCTGCAGCT CCGGAGTCAG CAGCTACTTC GGGAAAGTGT TCGCAGTGGG TCGGTACCAG 
GTCACCGTGG AGGAGCTGGT CGCTGAAGGA GGCTTCTCCG TTGTGTTTCT GGCTCGTACG 
CACAGCGGCG TCCGCTGCGC TCTGAAACGG ATGTACGTCA ACAACGTCCC GGATCTGAAT 
GTCTACAAGA GAGAGATCAC TATCATGAAG GAGCTCTCAG GCCACAAGAA CATCGTCGGC 
TACCTGGACT CTACAATCAG TGCCGTGTCC GACAGCGTCT GGGAGGTCCT GATCCTCATG 
GAGTACTGCA AAGCGGGTCA GGTGGTGAAG CAGATGAACC AGCGGTTGAA TGTGGGCTTC 
AGTGAGGCCG AGGTCCTTCA CATCTTCTGT GACACCTGTG AGGCCGTGGC CCGGCTGCAC 
CAATGCAAGA CGCCCGTCAT CCACAGAGAC CTGAAGGTTG AAAACATCCT GCTGAACGAC 
CAGGGGAACT ACGTGCTGTG TGACTTTGGA AGCGCGACTC ACAAGATTTT GCTGCCACAT 
AAAGATGGAG TCACTGCTGT GGAGGACGAG ATTAAAAAGT ACACGACCCT GTCATATCGG 
GCGCCGGAGA TGATTAACTT GTATGCAGGG ATAGCCATCA CCACTAAAGC TGATATATGG 
GCACTCGGAT GCTTGTTGTA CAAGCTGTGT TTCTTCGCTC TTCCATTTGG GGAGAGTCAA 
GTTGCCATAT GTGACGGAAC CTTTATCGTT CCAGATAACT CCAAATTCTC CTTCAAGTTG 
CACTGCTTAA TCAGATATAT GCTCGAACCA GATCAGGAGA AGAGACCAGA CATTTACCAA 
GTGTCCTACT TTGCCTTCAA GTTAGCTGGA AAAGACTGTC CGGTGCCAAA TCTCTTTAAC 
TCTCCGATCC CCACGTCGCT CCCAGAGCCT CTAACGGCCA GCGAGGTCGC TGCTAAGAAA 
AGCCAGACAA AAGCCAGGAT AACGGAATCT GTCGGCCCAA CGGAAACATC AATAGCTCCC 
AGGCAGAGGC CGAAGGCGGT GAACAGTAAC GTTCTGCCCC TCGCCAGCAC CGTCACCCCC 
GTGAAGATGA CCATGCCTTC AGCCCCCGTC AGCAATGGAC AGAAAGCTCC CACCCCTGTC 
TCCGGGCAGC TGTCCATGCA GCCCCAGCCC AGCAGTCAGC AGCACCGAGT CCTGCAGCAG 
CTGCAGCCCG GTGACCTGCG TCTGCAGCAA CTTCAGCAGC ATCAACAACA ACAACAACAA 
CAAGCCGTGC AACAGCAACA TGTTAATGCA CAGCAACTCC AATACCTCCA GTACCAACAG 
GCTGTGCAGC AGTCCCTCCA GCTCCAGCAG CAGCAGGCTC TGCTCCAGCA GCAGCAGCAG 
CAGCAGCAGA TGATGATGCA GCCGATGTAC CAGCAGCAGG TCACTCAGGC CCAGGCTCAG 
TATGCAGCCA TGTTGCACCA GTACCAACAG GCTTTTGTGC AGCAGCAGCA GCAGCATCAC 
CACCAACAAC AACATCATCA TCTACAGCAG CAGCAGCAGC TTCATCCCGC CCAGCAGCCT 
CTCCCCTACA TGTCCTCCCC TCTTGAGTTC CAGATCCCCC TCGGCTCCTA TAACACTACC 
ACCCCCTCTG CTGGACCTGC AGCAGGAAGT GGACAGTTGG GGGGAATATC ACCTGTGGAT 
CCCTTGTACA CGAACTCCAG AAACCCTCTT CTGGCCTCTG ATGGCACCAC CCCTCCCCCT 
CAGAGCTGCA ACAGCACAGT GAGTAACCCC CCCGACATGT CACGCTGGAA CCCTTTCGGA 
GAGGACAACT TCTCCAAGCT GACGGAGGAG GAGCTGATCG ACCGGGAGTT CGACATGCTC 
CGAGCAAACA AACCTGTGGA GAGAACAGCC TGCGTGGAAG CGGACCGACC GCAGCCCGCT 
GCCATCAAGC CCCTCCCACC CGAGGACCTG TTCGGCTCAG TCCCGTTTGT GGCCAGCGCA 
GGATCTACCA CCGTGACGCA GACTGAGCAG AGCGGCGAGG ACGTCAGCGT TCCTCCTCCC 
GTCTCGACCT CCGCAGACGA TCTGCAGGCG CCCGCGGTCA AGGAGCACAA ACCCGGCAGG 
AAGAGTAACT GCAGATCGGG AGAAGAGTCC GACAGCGACT TCGAGTCTGA CCCTCCTTCA 
CCAAAAAGCA GCGAGGAAGA GGAGCCGGAG GAGGACGAGG CTCTGAACAG TGAACATGGT 
GAAGACACAG AGCCAGAGAA TATAGGACAG AGGCCTCTGC TGATGGATTC TGAGGAAGAG 
GAGGAGGAGG ACAAGCACAG TTCTGACTCG GACTACAACC CCAGCAGAGT CAAGGGTCGC 
TCGAAGAAGT CTGGTAAATC CGCTTCTGCT GCCACCAGGA GGAGCCCTGA AGGAGATTCT 
GCCATGACCC TGATCACCCC TCCCGAAACG CCCAGCGGGG CGAGAGCCGC CCCAGCTGAG 
GAAGCTGTGG ATGTGTTTGG GTCCATTCCC TTCGTCAGAG GAGCTTCACC GGTTGAGCCC 
TCGGAGAACG CGGACATCTT TGCCAAAGCG CCTTTCAGAC AAGTCAGCCA AGAGCCGCGA 
GCAGCCGATG AGTTTGATGT TTTTACCAAA GCTCCGTTCA ACCGGAACCT CTCCAAGTCT 
GGCAAGAGCG GCAGCGAAGC TCCCGCGGGC CAATCGCCGC CCACCTCCCC CGAGGCCTTT 
GACATTTTTG GCTTCTCTCC CTTCCACCCG GGCCCCACGG CGCCACCTCC CGTGACCTCG 
AGGAGCGCGG ACGATATCTT TCAGTCGTCT TACGAGGAGT CGGCGAGTCC TCAGCAACAG 
AGGCTGAAGC AGCGCAGCCT CCAGAAGCTG TCGTCGCGGC AGCGGAAAAC CAAGCAAGAG 
GCCACCGCGG GGGGCAGCAA CGGCAAACGA CACCACGGTA CACCCACCGG GGGGCGCAAG 
ACTGGCAAGC CAACTTACCG CACCCCTGAG CGAGTCCGCA GGCACAAGAA GGTCGGCCGG 
AGAGACTCTC AGAGCAGCAA CGAGTTCCTC AGCACCTCCG ACTCTAAAGA GAACATCAGC 
GTTGATATAA CCATGGCTGA GGGGAAGGAC AAGGGAGCTT CTCTGCCGGT GGACGAGGCC 
CTTTTAGACC CGTTTGGAGC CAAACCTTTC CACCCTCCGG ACGGAAGCCG GCACAGCCAG 
TACCAAGGCC TCGTGGATAG TAAGAGTGAC ATGGGCACGG CCAACGGTAA GACCTGGACG 
GGCTCTCTTC ACGGAGCTCT TGGAGAGAGT AGAGATATGG ATGACTTCGG GGCAGTGCCC 
TTCACAGAAC TGGTCGTCCA CAGCGGACCC CAGCAGCAGC CGCCGCCGCA GCAGCAGCAG 
CAGGCGCCCT CGCAGCCGGG GGAGCTGGAC CCGTTCGGAG CTGCACCTTT TCCCTCGAAA 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019961315.1
CDS	909..4394
Translation	MKKFSRMPKSESGGLGGAGSGCSSGVSSYFGKVFAVGRYQVTVEELVAEGGFSVVFLARTHSGVRCALKRMYVNNVPDLNVYKREITIMKELSGHKNIVGYLDSTISAVSDSVWEVLILMEYCKAGQVVKQMNQRLNVGFSEAEVLHIFCDTCEAVARLHQCKTPVIHRDLKVENILLNDQGNYVLCDFGSATHKILLPHKDGVTAVEDEIKKYTTLSYRAPEMINLYAGIAITTKADIWALGCLLYKLCFFALPFGESQVAICDGTFIVPDNSKFSFKLHCLIRYMLEPDQEKRPDIYQVSYFAFKLAGKDCPVPNLFNSPIPTSLPEPLTASEVAAKKSQTKARITESVGPTETSIAPRQRPKAVNSNVLPLASTVTPVKMTMPSAPVSNGQKAPTPVSGQLSMQPQPSSQQHRVLQQLQPGDLRLQQLQQHQQQQQQQAVQQQHVNAQQLQYLQYQQAVQQSLQLQQQQALLQQQQQQQQMMMQPMYQQQVTQAQAQYAAMLHQYQQAFVQQQQQHHHQQQHHHLQQQQQLHPAQQPLPYMSSPLEFQIPLGSYNTTTPSAGPAAGSGQLGGISPVDPLYTNSRNPLLASDGTTPPPQSCNSTVSNPPDMSRWNPFGEDNFSKLTEEELIDREFDMLRANKPVERTACVEADRPQPAAIKPLPPEDLFGSVPFVASAGSTTVTQTEQSGEDVSVPPPVSTSADDLQAPAVKEHKPGRKSNCRSGEESDSDFESDPPSPKSSEEEEPEEDEALNSEHGEDTEPENIGQRPLLMDSEEEEEEDKHSSDSDYNPSRVKGRSKKSGKSASAATRRSPEGDSAMTLITPPETPSGARAAPAEEAVDVFGSIPFVRGASPVEPSENADIFAKAPFRQVSQEPRAADEFDVFTKAPFNRNLSKSGKSGSEAPAGQSPPTSPEAFDIFGFSPFHPGPTAPPPVTSRSADDIFQSSYEESASPQQQRLKQRSLQKLSSRQRKTKQEATAGGSNGKRHHGTPTGGRKTGKPTYRTPERVRRHKKVGRRDSQSSNEFLSTSDSKENISVDITMAEGKDKGASLPVDEALLDPFGAKPFHPPDGSRHSQYQGLVDSKSDMGTANGKTWTGSLHGALGESRDMDDFGAVPFTELVVHSGPQQQPPPQQQQQAPSQPGELDPFGAAPFPSKQ

Target ORF information:

RefSeq Version	XM_020105756.1
Organism	Paralichthys olivaceus(Japanese flounder)
Definition	Paralichthys olivaceus BMP2 inducible kinase (bmp2k), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020105756.1

ORF Insert Sequence:

ATGAAGAAGT TTTCCAGGAT GCCAAAGTCG GAAAGCGGCG GGCTCGGAGG GGCCGGGTCC 
GGCTGCAGCT CCGGAGTCAG CAGCTACTTC GGGAAAGTGT TCGCAGTGGG TCGGTACCAG 
GTCACCGTGG AGGAGCTGGT CGCTGAAGGA GGCTTCTCCG TTGTGTTTCT GGCTCGTACG 
CACAGCGGCG TCCGCTGCGC TCTGAAACGG ATGTACGTCA ACAACGTCCC GGATCTGAAT 
GTCTACAAGA GAGAGATCAC TATCATGAAG GAGCTCTCAG GCCACAAGAA CATCGTCGGC 
TACCTGGACT CTACAATCAG TGCCGTGTCC GACAGCGTCT GGGAGGTCCT GATCCTCATG 
GAGTACTGCA AAGCGGGTCA GGTGGTGAAG CAGATGAACC AGCGGTTGAA TGTGGGCTTC 
AGTGAGGCCG AGGTCCTTCA CATCTTCTGT GACACCTGTG AGGCCGTGGC CCGGCTGCAC 
CAATGCAAGA CGCCCGTCAT CCACAGAGAC CTGAAGGTTG AAAACATCCT GCTGAACGAC 
CAGGGGAACT ACGTGCTGTG TGACTTTGGA AGCGCGACTC ACAAGATTTT GCTGCCACAT 
AAAGATGGAG TCACTGCTGT GGAGGACGAG ATTAAAAAGT ACACGACCCT GTCATATCGG 
GCGCCGGAGA TGATTAACTT GTATGCAGGG ATAGCCATCA CCACTAAAGC TGATATATGG 
GCACTCGGAT GCTTGTTGTA CAAGCTGTGT TTCTTCGCTC TTCCATTTGG GGAGAGTCAA 
GTTGCCATAT GTGACGGAAC CTTTATCGTT CCAGATAACT CCAAATTCTC CTTCAAGTTG 
CACTGCTTAA TCAGATATAT GCTCGAACCA GATCAGGAGA AGAGACCAGA CATTTACCAA 
GTGTCCTACT TTGCCTTCAA GTTAGCTGGA AAAGACTGTC CGGTGCCAAA TCTCTTTAAC 
TCTCCGATCC CCACGTCGCT CCCAGAGCCT CTAACGGCCA GCGAGGTCGC TGCTAAGAAA 
AGCCAGACAA AAGCCAGGAT AACGGAATCT GTCGGCCCAA CGGAAACATC AATAGCTCCC 
AGGCAGAGGC CGAAGGCGGT GAACAGTAAC GTTCTGCCCC TCGCCAGCAC CGTCACCCCC 
GTGAAGATGA CCATGCCTTC AGCCCCCGTC AGCAATGGAC AGAAAGCTCC CACCCCTGTC 
TCCGGGCAGC TGTCCATGCA GCCCCAGCCC AGCAGTCAGC AGCACCGAGT CCTGCAGCAG 
CTGCAGCCCG GTGACCTGCG TCTGCAGCAA CTTCAGCAGC ATCAACAACA ACAACAACAA 
CAAGCCGTGC AACAGCAACA TGTTAATGCA CAGCAACTCC AATACCTCCA GTACCAACAG 
GCTGTGCAGC AGTCCCTCCA GCTCCAGCAG CAGCAGGCTC TGCTCCAGCA GCAGCAGCAG 
CAGCAGCAGA TGATGATGCA GCCGATGTAC CAGCAGCAGG TCACTCAGGC CCAGGCTCAG 
TATGCAGCCA TGTTGCACCA GTACCAACAG GCTTTTGTGC AGCAGCAGCA GCAGCATCAC 
CACCAACAAC AACATCATCA TCTACAGCAG CAGCAGCAGC TTCATCCCGC CCAGCAGCCT 
CTCCCCTACA TGTCCTCCCC TCTTGAGTTC CAGATCCCCC TCGGCTCCTA TAACACTACC 
ACCCCCTCTG CTGGACCTGC AGCAGGAAGT GGACAGTTGG GGGGAATATC ACCTGTGGAT 
CCCTTGTACA CGAACTCCAG AAACCCTCTT CTGGCCTCTG ATGGCACCAC CCCTCCCCCT 
CAGAGCTGCA ACAGCACAGT GAGTAACCCC CCCGACATGT CACGCTGGAA CCCTTTCGGA 
GAGGACAACT TCTCCAAGCT GACGGAGGAG GAGCTGATCG ACCGGGAGTT CGACATGCTC 
CGAGCAAACA AACCTGTGGA GAGAACAGCC TGCGTGGAAG CGGACCGACC GCAGCCCGCT 
GCCATCAAGC CCCTCCCACC CGAGGACCTG TTCGGCTCAG TCCCGTTTGT GGCCAGCGCA 
GGATCTACCA CCGTGACGCA GACTGAGCAG AGCGGCGAGG ACGTCAGCGT TCCTCCTCCC 
GTCTCGACCT CCGCAGACGA TCTGCAGGCG CCCGCGGTCA AGGAGCACAA ACCCGGCAGG 
AAGAGTAACT GCAGATCGGG AGAAGAGTCC GACAGCGACT TCGAGTCTGA CCCTCCTTCA 
CCAAAAAGCA GCGAGGAAGA GGAGCCGGAG GAGGACGAGG CTCTGAACAG TGAACATGGT 
GAAGACACAG AGCCAGAGAA TATAGGACAG AGGCCTCTGC TGATGGATTC TGAGGAAGAG 
GAGGAGGAGG ACAAGCACAG TTCTGACTCG GACTACAACC CCAGCAGAGT CAAGGGTCGC 
TCGAAGAAGT CTGGTAAATC CGCTTCTGCT GCCACCAGGA GGAGCCCTGA AGGAGATTCT 
GCCATGACCC TGATCACCCC TCCCGAAACG CCCAGCGGGG CGAGAGCCGC CCCAGCTGAG 
GAAGCTGTGG ATGTGTTTGG GTCCATTCCC TTCGTCAGAG GAGCTTCACC GGTTGAGCCC 
TCGGAGAACG CGGACATCTT TGCCAAAGCG CCTTTCAGAC AAGTCAGCCA AGAGCCGCGA 
GCAGCCGATG AGTTTGATGT TTTTACCAAA GCTCCGTTCA ACCGGAACCT CTCCAAGTCT 
GGCAAGAGCG GCAGCGAAGC TCCCGCGGGC CAATCGCCGC CCACCTCCCC CGAGGCCTTT 
GACATTTTTG GCTTCTCTCC CTTCCACCCG GGCCCCACGG CGCCACCTCC CGTGACCTCG 
AGGAGCGCGG ACGATATCTT TCAGTCGTCT TACGAGGAGT CGGCGAGTCC TCAGCAACAG 
AGGCTGAAGC AGCGCAGCCT CCAGAAGCTG TCGTCGCGGC AGCGGAAAAC CAAGCAAGAG 
GCCACCGCGG GGGGCAGCAA CGGCAAACGA CACCACGGTA CACCCACCGG GGGGCGCAAG 
ACTGGCAAGC CAACTTACCG CACCCCTGAG CGAGTCCGCA GGCACAAGAA GGTCGGCCGG 
AGAGACTCTC AGAGCAGCAA CGAGTTCCTC AGCACCTCCG ACTCTAAAGA GAACATCAGC 
GTTGATATAA CCATGGCTGA GGGGAAGGAC AAGGGAGCTT CTCTGCCGGT GGACGAGGCC 
CTTTTAGACC CGTTTGGAGC CAAACCTTTC CACCCTCCGG ACGGAAGCCG GCACAGCCAG 
TACCAAGGCC TCGTGGATAG TAAGAGTGAC ATGGGCACGG CCAACGGTAA GACCTGGACG 
GGCTCTCTTC ACGGAGCTCT TGGAGAGAGT AGAGATATGG ATGACTTCGG GGCAGTGCCC 
TTCACAGAAC TGGTCGTCCA CAGCGGACCC CAGCAGCAGC CGCCGCCGCA GCAGCAGCAG 
CAGGCGCCCT CGCAGCCGGG GGAGCTGGAC CCGTTCGGAG CTGCACCTTT TCCCTCGAAA 
CAGTGA