LOC109639892 cDNA ORF clone, Paralichthys olivaceus(Japanese flounder)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC109639892
Entrez Gene ID	109639892
Full Name	eukaryotic translation initiation factor 4 gamma 1-like
Gene Type	protein-coding
Organism	Paralichthys olivaceus(Japanese flounder)

mRNA and Protein(s)

mRNA	Protein	Name
XM_020103598.1	XP_019959157.1	eukaryotic translation initiation factor 4 gamma 1-like isoform X1
XM_020103607.1	XP_019959166.1	eukaryotic translation initiation factor 4 gamma 1-like isoform X2

Pathways from BioSystems
Gene Ontology

The following LOC109639892 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC109639892 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPa232587	XM_020103598.1 Latest version!	Paralichthys olivaceus eukaryotic translation initiation factor 4 gamma 1-like (LOC109639892), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OPa232588	XM_020103607.1 Latest version!	Paralichthys olivaceus eukaryotic translation initiation factor 4 gamma 1-like (LOC109639892), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC109639892 ( XM_020103598.1 ) cDNA ORF clone, Paralichthys olivaceus(Japanese flounder) -> XP_019959157.1 Paralichthys olivaceus eukaryotic translation initiation factor 4 gamma 1-like (LOC109639892), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPa232587
Clone ID Related Accession (Same CDS sequence)	XM_020103598.1
Accession Version	XM_020103598.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5181bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-07
Organism	Paralichthys olivaceus(Japanese flounder)
Product	eukaryotic translation initiation factor 4 gamma 1-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017859643.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Paralichthys olivaceus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACAAGC CACCACAGCC TATAACGGGA CCCACCTCTG TCCCAAACCC TGCCCCTTCC 
CCTGGATTGA CACAGGCTGC CTACGGCCCA GGACAGCCCC CTTCTCTTGT TTTTGCCACC 
CCTCCACCTC CACAAATGAA CTCTGCACCC CAGACAAGAC AGTTTGCCGC AGGGCCCCGT 
ACTTTACACC AACAGGGTGG ATACAGAGCA TTACAGACTT ACTATCCAAA CCGACCAGCC 
ATGGCAACCA ATGCTCCAAG AGTACAGACA AGTAGTGGGC CCAGACCTGT TGGACCTACC 
CATGTCTACC CGCCCAGCTC CCAGATGATG ATGATTTCCC AGCAGCAGCT GTCTTTTGCT 
GGATCCCCTC AGGGCTACTT CATCCCCCCT CCTGGACAGT ACCGGGCCCC ATACATGCCT 
CCAACTCAGC AGTATCCTGT GACCAGCGGT ACAGCTGCCT TCTATCCAGG AACCAGCCCT 
GCTGAATACC CCGCTTATGC AGGAGCATAT TATCCAGCAC AGCCACAGTA CCCTCCATCG 
GTCCAGCCTG CACCGGTCAT GATCAACCCC ACCCAGCAAC AGCCACAAGC CCCGCCTCCT 
CAGCAACCAC CGGCACAGGC ACAAGGCCCA CTAAAGAGAG AACGCAAACC GATAAGAATA 
CGAGACCCCA ACCAGGGCGG GCGTGATATC ACAGAAGAGA TAATGTCAGG TGGAAGATCC 
ACCACCACAC CGACTCCCCC ACAGGCCTCC ATAGCAGATT TAAGTCCTGC ACAGACCAAC 
GGTGAAGTTG TACAGCCTGT CGCTACAGTG ACGAGACAAG ATGATAACAT GGAGCCTGCT 
GTAAGCGCTG AAACCCCGCC GCCTCCTGCC ACAGCAGACA CAGAGCCTGT GCTGGAGGCC 
AAACAGGAAG TGGACAGCCA GATGTTGGTG TGTGCTGAAT CAGATGTACC ATCTGTAGCC 
CCCACAGCTG CTACTGAGGA TCCATCCCCA TTGATAAATG ATGAGCAGTC TCCCTCTTCC 
CTCCCTCCTG CAGTAGCAGC TACTACTACT ACTACTCCTG CCGAGGCAGA AAATAATGAC 
GTTACTAAAG TCAGTGACAC AGTAGATGCT CCTGTCGGCC CTTCGGCATC AATAGCAACA 
CAAGAGGCAC CTGTTAAAAT AGAGGAACCA CAGGCTGCCC CAGCTCCACC TGAGAAGGCA 
CCAGAACAGG AAGAAAAGAT AACAGAGGAA GTGAAGACAG TTGAAAAAGA AGAGCAGGTG 
GCTAGTTCCA AGTTGGAGCC TGTAGTTGAA GATGCACCAA TAGTTACCCC TGTTGAAGTG 
GCAGAAGAAG AAGCAGTGAC TAAGACGTCA ACTGAAATGT CTCATCCTCC ACCCTCCAAA 
CAGGAACTAG TTGCTCCACA GACCCAGAGC CCTGCTCCGA ACTCTGGCCC TGAACCCGAA 
CCCACTCTGG CTGAAACAGC AGAGCCTCTC GTCTCCAACG GTCTTCCTCA GAACATTGAG 
GAACTTTCTG AGGATGTTAC GCTTTCAGAC ACTACATCCC TTGACAAGCC AGATGCCTCT 
CAATCTCAGG AATCCACACC TGTAGCTAAA ATGGCAGCAC TAACCCAGGA AGAGGAGGAA 
GAGGAGGAGA TTGAGGTGGA GAAGGAGGAG ATTAAAGGGG AAGAGAAGGA GAAAATTGAG 
GATGTCCCTC CTACCCCTAT CAGCTGCCCC ACAGAGGAAT CTACTACGCA AGCTGCTACG 
TCTCTGCCAA AGAAGAGGAG GAACATGAAG GAGCTCAACA AAAAGGAGGC CATTGGAGAC 
CTCCTGGATG CATTCACAGA TGAGCAGGAG GCCAAGCCGG CTTCTGAACC TTTGCCCACT 
CCGGCCGACC CTGTCCCTGT TGCTCCAGCT GAACCTCCTG CTGAGGCTGC AGATGAGACT 
TGGGAAGAGA AAGAGGACAA GCAGAACGCA GAACCTGACA GGCCCAAAGC CTCGCCTGAG 
CCAACTGAGC AGAAATACCA GTACAAAGAA GAACAATGGA AGCCGATAAA CCCAGAGGAG 
AAGAAGCAGT ACGACAGGGA TTTCCTCCTG GGCTTCCAGT TCATCAGCGC CAGTATGAAC 
AAACCCGAGG GTCTGCCCAC TATCAGTGAT GTGGTCCTGG ACAAGGTGAA CAAGACTCCT 
CTGCGACCTG CTGACCCAGC TCGACTAATT AACGTTGGTC CTGATTTTAC TCCCTCCTAT 
TTGGGTAATC TTGGGAGCAG ATCAGTGGGA GGACCAAGAG GCCCACCTCC GGGGCCACGT 
CGCTCCCAGC AGGGTCAGCG TAAAGAACCT AGGAAAATCA TCATCAGCAG CATGTCCCTC 
AATGATGACG TGCAACTCAA CAAGGCTGAG AAGGCCTGGA AGCCCTCAGT GAAGAAGTCC 
ACTCGCAGCC GCTGTGCTGA GGAAACCGAT ACAGATGACA GTGAACAGGG CAAGACTAAA 
GAGCTGTTCA AGCGTCTGCG CAGTATCCTC AACAAGTTGA CTCCTCAGAA GTTTCAGGAG 
CTGATGAAGC AGGTGACAGA TCTGGCGATA GATACGGAGG AGAGGCTGAA GGGAGCCATT 
GACCTCATCT TTGAGAAGGC CATCTCAGAG CCCAACTTCT CTGTGGCCTA CGCCAATATG 
TGCCGCTGCC TTATGGGATT GAAAGTCCCA ACTACGGACA AGCCAGGAGT TTCTGCCAAC 
TTCCGTAAAC TGCTGCTTAA CCGCTGTCAG AAAGAGTTTG AGAAGGACCA GGATGATGAT 
GAGATCTTTG AGAAGAAGCA AAAAGAGTTG GAGGCTTCCA AAGATGATGA AGAACGTGAA 
CGCTTGAGGG TGGAGCTGAA TGATTCCAGA GACCAGGCCC GCCGGCGCTC ACTGGGAAAC 
ATAAAGTTCA TAGGCGAGCT CTTCAAGCTG AAGATGCTGA CTGAGGCCAT CATGCACGAC 
TGCGTAGTGA AACTACTAAA GAATCATGAT GAAGAGTCTC TGGAGTGTCT CTGCAGGCTG 
TTCTCCACTA TTGGAAAAGA TCTGGACTTT GAAAAGGCCA AGCCTCGTAT GGATCAGTAT 
TTTAACCAGA TGGACAAGAT CATCAAAGAG AGAAAGACTT CATCCAGAAT CCGCTTCATG 
CTTCAAGACG TCCTGGACCT CAGAAAGTGC AACTGGGTGC CTCGCAGAGG AGACCAGGGT 
CCTAAAACAA TTGACCAAAT CCACAAGGAG GCAGAGATGG AGGAGCACAG GGAGCACATC 
AAAGTGCAGC AGCTCATGTC CAAGAAAGAG GCAGGTGGAG GTAGGATGGG AGGAGGAGGA 
GGGGGCATGG GGGGCCGAGG GTCTCACACA CCAGGAGGTG GCCGGAATAA CCCGCCTCAG 
GATGAGGGAT GGAACACGGT GCCCATCTCC AAGAACAGAC CCATCGACAC CACCCGCCTT 
AGCAAGATCA CCAAGCCCGG TGCTCTGGAC TTCAGCAACC AACTGTTGGC TCCAGGTGGC 
AAAGGCATGT GGGGCAGCTG GGGCAAAGGC AGCAGTGGAG GAACTGGAGC TAAACCCGCC 
AGTGGAGAGC AAGATGCTGG TCGTCCAGCT ACTAGCACCC TCAACCGATT CTCTGCCCTG 
CAGTCTGGTT CTCTGTCTTC ATCAGACTCC GATCGCAGAG CTCCTCAGAG GTCAAGCTCC 
AGTCGCGAGC GCGGTGGAGA CAGGGGCGAC CGCGAGAAGG ATCGCTTTGA CCGATTTGAT 
CGCAATGAGG GACGGGAAGG TCGTGACGAC AGGAGCACCC GCAACCAAAT CACGAAGAGA 
AGCTTCAGCA GAGAGTCGCA GGAGCGCGGA GGGAGGGTTG GAGACACCCG GACCCCGACT 
GAGCCTGTGC GTCGCGTCGC CAGCATGACT GACGACAGGG ACAGAGGAAG TCGGGATAGA 
GGAAGTCGGG ACAGGGGAGG CAGAGACCAA GGAAGCAGAG ACCGAGAAAG CCGGGACAGG 
GGAAGCAGAG ACCAAGGTAG CAGAGACAGA GGAAGTCGGG ACAGGGTTCC GAGCAAAGAT 
CTCACAGCTA AGCGTGAGAG CACCCCCACT CCTCCTCCTT CTCTCTCTAA ACCTGCTCTG 
ACTGAGGAGG AGGTGGAGAA GAAGTCAAGC GCCATCATTG AAGAATACCT CCACATCAAT 
GACTTGAAGG AGGCGCTGCA GTGTGTGACA GAGCTCAACA GTGCCTCGCT GCTGTACGTG 
TTTGTGCGGA ACGGCTTGGA GTCGACGCTG GAGCGCAGCA CAATCGCTAG AGAGCGCATG 
GGCCACTTGC TGCACCAACT TATAAAGGCA GGGACATTGC CCACACTGCA GTACTACAAA 
GGGCTAGAGG AGATCCTGGA GGTAGCCGAA GACATGGCCA TAGATATACC TCACATATGG 
CTCTACCTGG CTGAACTCAT TACCCCCATG CTCCATGAGG GAGGCATCCC TATGGGACAG 
CTCTTCAGGG AGATCTCAAA GCCTCTCCTG CCTCTGGGGA AGGCCGCTGT GCTGCTGGTC 
CAGATCCTTA AGCTGCTCTG CAACGGAATG ACCCCTAAGA AGGTCGGGGC CATGTGGACA 
GAGGACGGGC TGAGCTGGAG TGACTTCTTA CCCAAGGACG AGGACGTCAA CAAGTTTGTC 
ACTGAACAGC AGGTGGATTT CACCACAGGA GAAACGACGG AGTCACTGGA AGTGGTTCTT 
AAGAGAGCCC TGAGTGGAGA AGAGCTCAGT CAACAGCTGG ACAGACTCCT CGAGGATAAG 
GCCAACAACC AGCGCATCGA AGACTGGGTT GAGGCTAACT TAGAGGAGCA GCAGATGACT 
TCCAACCAGT TCGTAAGAGC ATTGATGACA GCAGTGTGCC AGTCCGCCAT CATATGTGAC 
AACCCGTACA GGGTGGATCC AAATCAGATC AAAGAGAGAG CCAGACTTCT GCAGAAATAC 
ATGTCTGACG AGCAGAAAGA GCTCCAGGCC CTTTACGCCC TCCAGGCACT GATGGTGCAT 
ATGGAGCAGC CTGCGAACCT GCTGCGGATG TTCTTCGACG CCTTGTACGA TGAGGACGTC 
ATTAAAGAAG AGGCCTTCTA TAAGTGGGAA TCAAGCAAAG ACCCTGCAGA GCAGACGGGC 
AAAGGTGTGG CCTTGAAGTC TGTCACCGCC TTCTTCACCT GGCTCCGCGA GGCAGAGGAG 
GAGGAGTCCG ACAAGGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019959157.1
CDS	510..5690
Translation	MNKPPQPITGPTSVPNPAPSPGLTQAAYGPGQPPSLVFATPPPPQMNSAPQTRQFAAGPRTLHQQGGYRALQTYYPNRPAMATNAPRVQTSSGPRPVGPTHVYPPSSQMMMISQQQLSFAGSPQGYFIPPPGQYRAPYMPPTQQYPVTSGTAAFYPGTSPAEYPAYAGAYYPAQPQYPPSVQPAPVMINPTQQQPQAPPPQQPPAQAQGPLKRERKPIRIRDPNQGGRDITEEIMSGGRSTTTPTPPQASIADLSPAQTNGEVVQPVATVTRQDDNMEPAVSAETPPPPATADTEPVLEAKQEVDSQMLVCAESDVPSVAPTAATEDPSPLINDEQSPSSLPPAVAATTTTTPAEAENNDVTKVSDTVDAPVGPSASIATQEAPVKIEEPQAAPAPPEKAPEQEEKITEEVKTVEKEEQVASSKLEPVVEDAPIVTPVEVAEEEAVTKTSTEMSHPPPSKQELVAPQTQSPAPNSGPEPEPTLAETAEPLVSNGLPQNIEELSEDVTLSDTTSLDKPDASQSQESTPVAKMAALTQEEEEEEEIEVEKEEIKGEEKEKIEDVPPTPISCPTEESTTQAATSLPKKRRNMKELNKKEAIGDLLDAFTDEQEAKPASEPLPTPADPVPVAPAEPPAEAADETWEEKEDKQNAEPDRPKASPEPTEQKYQYKEEQWKPINPEEKKQYDRDFLLGFQFISASMNKPEGLPTISDVVLDKVNKTPLRPADPARLINVGPDFTPSYLGNLGSRSVGGPRGPPPGPRRSQQGQRKEPRKIIISSMSLNDDVQLNKAEKAWKPSVKKSTRSRCAEETDTDDSEQGKTKELFKRLRSILNKLTPQKFQELMKQVTDLAIDTEERLKGAIDLIFEKAISEPNFSVAYANMCRCLMGLKVPTTDKPGVSANFRKLLLNRCQKEFEKDQDDDEIFEKKQKELEASKDDEERERLRVELNDSRDQARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLFSTIGKDLDFEKAKPRMDQYFNQMDKIIKERKTSSRIRFMLQDVLDLRKCNWVPRRGDQGPKTIDQIHKEAEMEEHREHIKVQQLMSKKEAGGGRMGGGGGGMGGRGSHTPGGGRNNPPQDEGWNTVPISKNRPIDTTRLSKITKPGALDFSNQLLAPGGKGMWGSWGKGSSGGTGAKPASGEQDAGRPATSTLNRFSALQSGSLSSSDSDRRAPQRSSSSRERGGDRGDREKDRFDRFDRNEGREGRDDRSTRNQITKRSFSRESQERGGRVGDTRTPTEPVRRVASMTDDRDRGSRDRGSRDRGGRDQGSRDRESRDRGSRDQGSRDRGSRDRVPSKDLTAKRESTPTPPPSLSKPALTEEEVEKKSSAIIEEYLHINDLKEALQCVTELNSASLLYVFVRNGLESTLERSTIARERMGHLLHQLIKAGTLPTLQYYKGLEEILEVAEDMAIDIPHIWLYLAELITPMLHEGGIPMGQLFREISKPLLPLGKAAVLLVQILKLLCNGMTPKKVGAMWTEDGLSWSDFLPKDEDVNKFVTEQQVDFTTGETTESLEVVLKRALSGEELSQQLDRLLEDKANNQRIEDWVEANLEEQQMTSNQFVRALMTAVCQSAIICDNPYRVDPNQIKERARLLQKYMSDEQKELQALYALQALMVHMEQPANLLRMFFDALYDEDVIKEEAFYKWESSKDPAEQTGKGVALKSVTAFFTWLREAEEEESDKE

Target ORF information:

RefSeq Version	XM_020103598.1
Organism	Paralichthys olivaceus(Japanese flounder)
Definition	Paralichthys olivaceus eukaryotic translation initiation factor 4 gamma 1-like (LOC109639892), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020103598.1

ORF Insert Sequence:

ATGAACAAGC CACCACAGCC TATAACGGGA CCCACCTCTG TCCCAAACCC TGCCCCTTCC 
CCTGGATTGA CACAGGCTGC CTACGGCCCA GGACAGCCCC CTTCTCTTGT TTTTGCCACC 
CCTCCACCTC CACAAATGAA CTCTGCACCC CAGACAAGAC AGTTTGCCGC AGGGCCCCGT 
ACTTTACACC AACAGGGTGG ATACAGAGCA TTACAGACTT ACTATCCAAA CCGACCAGCC 
ATGGCAACCA ATGCTCCAAG AGTACAGACA AGTAGTGGGC CCAGACCTGT TGGACCTACC 
CATGTCTACC CGCCCAGCTC CCAGATGATG ATGATTTCCC AGCAGCAGCT GTCTTTTGCT 
GGATCCCCTC AGGGCTACTT CATCCCCCCT CCTGGACAGT ACCGGGCCCC ATACATGCCT 
CCAACTCAGC AGTATCCTGT GACCAGCGGT ACAGCTGCCT TCTATCCAGG AACCAGCCCT 
GCTGAATACC CCGCTTATGC AGGAGCATAT TATCCAGCAC AGCCACAGTA CCCTCCATCG 
GTCCAGCCTG CACCGGTCAT GATCAACCCC ACCCAGCAAC AGCCACAAGC CCCGCCTCCT 
CAGCAACCAC CGGCACAGGC ACAAGGCCCA CTAAAGAGAG AACGCAAACC GATAAGAATA 
CGAGACCCCA ACCAGGGCGG GCGTGATATC ACAGAAGAGA TAATGTCAGG TGGAAGATCC 
ACCACCACAC CGACTCCCCC ACAGGCCTCC ATAGCAGATT TAAGTCCTGC ACAGACCAAC 
GGTGAAGTTG TACAGCCTGT CGCTACAGTG ACGAGACAAG ATGATAACAT GGAGCCTGCT 
GTAAGCGCTG AAACCCCGCC GCCTCCTGCC ACAGCAGACA CAGAGCCTGT GCTGGAGGCC 
AAACAGGAAG TGGACAGCCA GATGTTGGTG TGTGCTGAAT CAGATGTACC ATCTGTAGCC 
CCCACAGCTG CTACTGAGGA TCCATCCCCA TTGATAAATG ATGAGCAGTC TCCCTCTTCC 
CTCCCTCCTG CAGTAGCAGC TACTACTACT ACTACTCCTG CCGAGGCAGA AAATAATGAC 
GTTACTAAAG TCAGTGACAC AGTAGATGCT CCTGTCGGCC CTTCGGCATC AATAGCAACA 
CAAGAGGCAC CTGTTAAAAT AGAGGAACCA CAGGCTGCCC CAGCTCCACC TGAGAAGGCA 
CCAGAACAGG AAGAAAAGAT AACAGAGGAA GTGAAGACAG TTGAAAAAGA AGAGCAGGTG 
GCTAGTTCCA AGTTGGAGCC TGTAGTTGAA GATGCACCAA TAGTTACCCC TGTTGAAGTG 
GCAGAAGAAG AAGCAGTGAC TAAGACGTCA ACTGAAATGT CTCATCCTCC ACCCTCCAAA 
CAGGAACTAG TTGCTCCACA GACCCAGAGC CCTGCTCCGA ACTCTGGCCC TGAACCCGAA 
CCCACTCTGG CTGAAACAGC AGAGCCTCTC GTCTCCAACG GTCTTCCTCA GAACATTGAG 
GAACTTTCTG AGGATGTTAC GCTTTCAGAC ACTACATCCC TTGACAAGCC AGATGCCTCT 
CAATCTCAGG AATCCACACC TGTAGCTAAA ATGGCAGCAC TAACCCAGGA AGAGGAGGAA 
GAGGAGGAGA TTGAGGTGGA GAAGGAGGAG ATTAAAGGGG AAGAGAAGGA GAAAATTGAG 
GATGTCCCTC CTACCCCTAT CAGCTGCCCC ACAGAGGAAT CTACTACGCA AGCTGCTACG 
TCTCTGCCAA AGAAGAGGAG GAACATGAAG GAGCTCAACA AAAAGGAGGC CATTGGAGAC 
CTCCTGGATG CATTCACAGA TGAGCAGGAG GCCAAGCCGG CTTCTGAACC TTTGCCCACT 
CCGGCCGACC CTGTCCCTGT TGCTCCAGCT GAACCTCCTG CTGAGGCTGC AGATGAGACT 
TGGGAAGAGA AAGAGGACAA GCAGAACGCA GAACCTGACA GGCCCAAAGC CTCGCCTGAG 
CCAACTGAGC AGAAATACCA GTACAAAGAA GAACAATGGA AGCCGATAAA CCCAGAGGAG 
AAGAAGCAGT ACGACAGGGA TTTCCTCCTG GGCTTCCAGT TCATCAGCGC CAGTATGAAC 
AAACCCGAGG GTCTGCCCAC TATCAGTGAT GTGGTCCTGG ACAAGGTGAA CAAGACTCCT 
CTGCGACCTG CTGACCCAGC TCGACTAATT AACGTTGGTC CTGATTTTAC TCCCTCCTAT 
TTGGGTAATC TTGGGAGCAG ATCAGTGGGA GGACCAAGAG GCCCACCTCC GGGGCCACGT 
CGCTCCCAGC AGGGTCAGCG TAAAGAACCT AGGAAAATCA TCATCAGCAG CATGTCCCTC 
AATGATGACG TGCAACTCAA CAAGGCTGAG AAGGCCTGGA AGCCCTCAGT GAAGAAGTCC 
ACTCGCAGCC GCTGTGCTGA GGAAACCGAT ACAGATGACA GTGAACAGGG CAAGACTAAA 
GAGCTGTTCA AGCGTCTGCG CAGTATCCTC AACAAGTTGA CTCCTCAGAA GTTTCAGGAG 
CTGATGAAGC AGGTGACAGA TCTGGCGATA GATACGGAGG AGAGGCTGAA GGGAGCCATT 
GACCTCATCT TTGAGAAGGC CATCTCAGAG CCCAACTTCT CTGTGGCCTA CGCCAATATG 
TGCCGCTGCC TTATGGGATT GAAAGTCCCA ACTACGGACA AGCCAGGAGT TTCTGCCAAC 
TTCCGTAAAC TGCTGCTTAA CCGCTGTCAG AAAGAGTTTG AGAAGGACCA GGATGATGAT 
GAGATCTTTG AGAAGAAGCA AAAAGAGTTG GAGGCTTCCA AAGATGATGA AGAACGTGAA 
CGCTTGAGGG TGGAGCTGAA TGATTCCAGA GACCAGGCCC GCCGGCGCTC ACTGGGAAAC 
ATAAAGTTCA TAGGCGAGCT CTTCAAGCTG AAGATGCTGA CTGAGGCCAT CATGCACGAC 
TGCGTAGTGA AACTACTAAA GAATCATGAT GAAGAGTCTC TGGAGTGTCT CTGCAGGCTG 
TTCTCCACTA TTGGAAAAGA TCTGGACTTT GAAAAGGCCA AGCCTCGTAT GGATCAGTAT 
TTTAACCAGA TGGACAAGAT CATCAAAGAG AGAAAGACTT CATCCAGAAT CCGCTTCATG 
CTTCAAGACG TCCTGGACCT CAGAAAGTGC AACTGGGTGC CTCGCAGAGG AGACCAGGGT 
CCTAAAACAA TTGACCAAAT CCACAAGGAG GCAGAGATGG AGGAGCACAG GGAGCACATC 
AAAGTGCAGC AGCTCATGTC CAAGAAAGAG GCAGGTGGAG GTAGGATGGG AGGAGGAGGA 
GGGGGCATGG GGGGCCGAGG GTCTCACACA CCAGGAGGTG GCCGGAATAA CCCGCCTCAG 
GATGAGGGAT GGAACACGGT GCCCATCTCC AAGAACAGAC CCATCGACAC CACCCGCCTT 
AGCAAGATCA CCAAGCCCGG TGCTCTGGAC TTCAGCAACC AACTGTTGGC TCCAGGTGGC 
AAAGGCATGT GGGGCAGCTG GGGCAAAGGC AGCAGTGGAG GAACTGGAGC TAAACCCGCC 
AGTGGAGAGC AAGATGCTGG TCGTCCAGCT ACTAGCACCC TCAACCGATT CTCTGCCCTG 
CAGTCTGGTT CTCTGTCTTC ATCAGACTCC GATCGCAGAG CTCCTCAGAG GTCAAGCTCC 
AGTCGCGAGC GCGGTGGAGA CAGGGGCGAC CGCGAGAAGG ATCGCTTTGA CCGATTTGAT 
CGCAATGAGG GACGGGAAGG TCGTGACGAC AGGAGCACCC GCAACCAAAT CACGAAGAGA 
AGCTTCAGCA GAGAGTCGCA GGAGCGCGGA GGGAGGGTTG GAGACACCCG GACCCCGACT 
GAGCCTGTGC GTCGCGTCGC CAGCATGACT GACGACAGGG ACAGAGGAAG TCGGGATAGA 
GGAAGTCGGG ACAGGGGAGG CAGAGACCAA GGAAGCAGAG ACCGAGAAAG CCGGGACAGG 
GGAAGCAGAG ACCAAGGTAG CAGAGACAGA GGAAGTCGGG ACAGGGTTCC GAGCAAAGAT 
CTCACAGCTA AGCGTGAGAG CACCCCCACT CCTCCTCCTT CTCTCTCTAA ACCTGCTCTG 
ACTGAGGAGG AGGTGGAGAA GAAGTCAAGC GCCATCATTG AAGAATACCT CCACATCAAT 
GACTTGAAGG AGGCGCTGCA GTGTGTGACA GAGCTCAACA GTGCCTCGCT GCTGTACGTG 
TTTGTGCGGA ACGGCTTGGA GTCGACGCTG GAGCGCAGCA CAATCGCTAG AGAGCGCATG 
GGCCACTTGC TGCACCAACT TATAAAGGCA GGGACATTGC CCACACTGCA GTACTACAAA 
GGGCTAGAGG AGATCCTGGA GGTAGCCGAA GACATGGCCA TAGATATACC TCACATATGG 
CTCTACCTGG CTGAACTCAT TACCCCCATG CTCCATGAGG GAGGCATCCC TATGGGACAG 
CTCTTCAGGG AGATCTCAAA GCCTCTCCTG CCTCTGGGGA AGGCCGCTGT GCTGCTGGTC 
CAGATCCTTA AGCTGCTCTG CAACGGAATG ACCCCTAAGA AGGTCGGGGC CATGTGGACA 
GAGGACGGGC TGAGCTGGAG TGACTTCTTA CCCAAGGACG AGGACGTCAA CAAGTTTGTC 
ACTGAACAGC AGGTGGATTT CACCACAGGA GAAACGACGG AGTCACTGGA AGTGGTTCTT 
AAGAGAGCCC TGAGTGGAGA AGAGCTCAGT CAACAGCTGG ACAGACTCCT CGAGGATAAG 
GCCAACAACC AGCGCATCGA AGACTGGGTT GAGGCTAACT TAGAGGAGCA GCAGATGACT 
TCCAACCAGT TCGTAAGAGC ATTGATGACA GCAGTGTGCC AGTCCGCCAT CATATGTGAC 
AACCCGTACA GGGTGGATCC AAATCAGATC AAAGAGAGAG CCAGACTTCT GCAGAAATAC 
ATGTCTGACG AGCAGAAAGA GCTCCAGGCC CTTTACGCCC TCCAGGCACT GATGGTGCAT 
ATGGAGCAGC CTGCGAACCT GCTGCGGATG TTCTTCGACG CCTTGTACGA TGAGGACGTC 
ATTAAAGAAG AGGCCTTCTA TAAGTGGGAA TCAAGCAAAG ACCCTGCAGA GCAGACGGGC 
AAAGGTGTGG CCTTGAAGTC TGTCACCGCC TTCTTCACCT GGCTCCGCGA GGCAGAGGAG 
GAGGAGTCCG ACAAGGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC109639892 ( XM_020103607.1 ) cDNA ORF clone, Paralichthys olivaceus(Japanese flounder) -> XP_019959166.1 Paralichthys olivaceus eukaryotic translation initiation factor 4 gamma 1-like (LOC109639892), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPa232588
Clone ID Related Accession (Same CDS sequence)	XM_020103607.1
Accession Version	XM_020103607.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5127bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-07
Organism	Paralichthys olivaceus(Japanese flounder)
Product	eukaryotic translation initiation factor 4 gamma 1-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017859643.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Paralichthys olivaceus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACAAGC CACCACAGCC TATAACGGGA CCCACCTCTG TCCCAAACCC TGCCCCTTCC 
CCTGGATTGA CACAGGCTGC CTACGGCCCA GGACAGCCCC CTTCTCTTGT TTTTGCCACC 
CCTCCACCTC CACAAATGAA CTCTGCACCC CAGACAAGAC AGACTTACTA TCCAAACCGA 
CCAGCCATGG CAACCAATGC TCCAAGAGTA CAGACAAGTA GTGGGCCCAG ACCTGTTGGA 
CCTACCCATG TCTACCCGCC CAGCTCCCAG ATGATGATGA TTTCCCAGCA GCAGCTGTCT 
TTTGCTGGAT CCCCTCAGGG CTACTTCATC CCCCCTCCTG GACAGTACCG GGCCCCATAC 
ATGCCTCCAA CTCAGCAGTA TCCTGTGACC AGCGGTACAG CTGCCTTCTA TCCAGGAACC 
AGCCCTGCTG AATACCCCGC TTATGCAGGA GCATATTATC CAGCACAGCC ACAGTACCCT 
CCATCGGTCC AGCCTGCACC GGTCATGATC AACCCCACCC AGCAACAGCC ACAAGCCCCG 
CCTCCTCAGC AACCACCGGC ACAGGCACAA GGCCCACTAA AGAGAGAACG CAAACCGATA 
AGAATACGAG ACCCCAACCA GGGCGGGCGT GATATCACAG AAGAGATAAT GTCAGGTGGA 
AGATCCACCA CCACACCGAC TCCCCCACAG GCCTCCATAG CAGATTTAAG TCCTGCACAG 
ACCAACGGTG AAGTTGTACA GCCTGTCGCT ACAGTGACGA GACAAGATGA TAACATGGAG 
CCTGCTGTAA GCGCTGAAAC CCCGCCGCCT CCTGCCACAG CAGACACAGA GCCTGTGCTG 
GAGGCCAAAC AGGAAGTGGA CAGCCAGATG TTGGTGTGTG CTGAATCAGA TGTACCATCT 
GTAGCCCCCA CAGCTGCTAC TGAGGATCCA TCCCCATTGA TAAATGATGA GCAGTCTCCC 
TCTTCCCTCC CTCCTGCAGT AGCAGCTACT ACTACTACTA CTCCTGCCGA GGCAGAAAAT 
AATGACGTTA CTAAAGTCAG TGACACAGTA GATGCTCCTG TCGGCCCTTC GGCATCAATA 
GCAACACAAG AGGCACCTGT TAAAATAGAG GAACCACAGG CTGCCCCAGC TCCACCTGAG 
AAGGCACCAG AACAGGAAGA AAAGATAACA GAGGAAGTGA AGACAGTTGA AAAAGAAGAG 
CAGGTGGCTA GTTCCAAGTT GGAGCCTGTA GTTGAAGATG CACCAATAGT TACCCCTGTT 
GAAGTGGCAG AAGAAGAAGC AGTGACTAAG ACGTCAACTG AAATGTCTCA TCCTCCACCC 
TCCAAACAGG AACTAGTTGC TCCACAGACC CAGAGCCCTG CTCCGAACTC TGGCCCTGAA 
CCCGAACCCA CTCTGGCTGA AACAGCAGAG CCTCTCGTCT CCAACGGTCT TCCTCAGAAC 
ATTGAGGAAC TTTCTGAGGA TGTTACGCTT TCAGACACTA CATCCCTTGA CAAGCCAGAT 
GCCTCTCAAT CTCAGGAATC CACACCTGTA GCTAAAATGG CAGCACTAAC CCAGGAAGAG 
GAGGAAGAGG AGGAGATTGA GGTGGAGAAG GAGGAGATTA AAGGGGAAGA GAAGGAGAAA 
ATTGAGGATG TCCCTCCTAC CCCTATCAGC TGCCCCACAG AGGAATCTAC TACGCAAGCT 
GCTACGTCTC TGCCAAAGAA GAGGAGGAAC ATGAAGGAGC TCAACAAAAA GGAGGCCATT 
GGAGACCTCC TGGATGCATT CACAGATGAG CAGGAGGCCA AGCCGGCTTC TGAACCTTTG 
CCCACTCCGG CCGACCCTGT CCCTGTTGCT CCAGCTGAAC CTCCTGCTGA GGCTGCAGAT 
GAGACTTGGG AAGAGAAAGA GGACAAGCAG AACGCAGAAC CTGACAGGCC CAAAGCCTCG 
CCTGAGCCAA CTGAGCAGAA ATACCAGTAC AAAGAAGAAC AATGGAAGCC GATAAACCCA 
GAGGAGAAGA AGCAGTACGA CAGGGATTTC CTCCTGGGCT TCCAGTTCAT CAGCGCCAGT 
ATGAACAAAC CCGAGGGTCT GCCCACTATC AGTGATGTGG TCCTGGACAA GGTGAACAAG 
ACTCCTCTGC GACCTGCTGA CCCAGCTCGA CTAATTAACG TTGGTCCTGA TTTTACTCCC 
TCCTATTTGG GTAATCTTGG GAGCAGATCA GTGGGAGGAC CAAGAGGCCC ACCTCCGGGG 
CCACGTCGCT CCCAGCAGGG TCAGCGTAAA GAACCTAGGA AAATCATCAT CAGCAGCATG 
TCCCTCAATG ATGACGTGCA ACTCAACAAG GCTGAGAAGG CCTGGAAGCC CTCAGTGAAG 
AAGTCCACTC GCAGCCGCTG TGCTGAGGAA ACCGATACAG ATGACAGTGA ACAGGGCAAG 
ACTAAAGAGC TGTTCAAGCG TCTGCGCAGT ATCCTCAACA AGTTGACTCC TCAGAAGTTT 
CAGGAGCTGA TGAAGCAGGT GACAGATCTG GCGATAGATA CGGAGGAGAG GCTGAAGGGA 
GCCATTGACC TCATCTTTGA GAAGGCCATC TCAGAGCCCA ACTTCTCTGT GGCCTACGCC 
AATATGTGCC GCTGCCTTAT GGGATTGAAA GTCCCAACTA CGGACAAGCC AGGAGTTTCT 
GCCAACTTCC GTAAACTGCT GCTTAACCGC TGTCAGAAAG AGTTTGAGAA GGACCAGGAT 
GATGATGAGA TCTTTGAGAA GAAGCAAAAA GAGTTGGAGG CTTCCAAAGA TGATGAAGAA 
CGTGAACGCT TGAGGGTGGA GCTGAATGAT TCCAGAGACC AGGCCCGCCG GCGCTCACTG 
GGAAACATAA AGTTCATAGG CGAGCTCTTC AAGCTGAAGA TGCTGACTGA GGCCATCATG 
CACGACTGCG TAGTGAAACT ACTAAAGAAT CATGATGAAG AGTCTCTGGA GTGTCTCTGC 
AGGCTGTTCT CCACTATTGG AAAAGATCTG GACTTTGAAA AGGCCAAGCC TCGTATGGAT 
CAGTATTTTA ACCAGATGGA CAAGATCATC AAAGAGAGAA AGACTTCATC CAGAATCCGC 
TTCATGCTTC AAGACGTCCT GGACCTCAGA AAGTGCAACT GGGTGCCTCG CAGAGGAGAC 
CAGGGTCCTA AAACAATTGA CCAAATCCAC AAGGAGGCAG AGATGGAGGA GCACAGGGAG 
CACATCAAAG TGCAGCAGCT CATGTCCAAG AAAGAGGCAG GTGGAGGTAG GATGGGAGGA 
GGAGGAGGGG GCATGGGGGG CCGAGGGTCT CACACACCAG GAGGTGGCCG GAATAACCCG 
CCTCAGGATG AGGGATGGAA CACGGTGCCC ATCTCCAAGA ACAGACCCAT CGACACCACC 
CGCCTTAGCA AGATCACCAA GCCCGGTGCT CTGGACTTCA GCAACCAACT GTTGGCTCCA 
GGTGGCAAAG GCATGTGGGG CAGCTGGGGC AAAGGCAGCA GTGGAGGAAC TGGAGCTAAA 
CCCGCCAGTG GAGAGCAAGA TGCTGGTCGT CCAGCTACTA GCACCCTCAA CCGATTCTCT 
GCCCTGCAGT CTGGTTCTCT GTCTTCATCA GACTCCGATC GCAGAGCTCC TCAGAGGTCA 
AGCTCCAGTC GCGAGCGCGG TGGAGACAGG GGCGACCGCG AGAAGGATCG CTTTGACCGA 
TTTGATCGCA ATGAGGGACG GGAAGGTCGT GACGACAGGA GCACCCGCAA CCAAATCACG 
AAGAGAAGCT TCAGCAGAGA GTCGCAGGAG CGCGGAGGGA GGGTTGGAGA CACCCGGACC 
CCGACTGAGC CTGTGCGTCG CGTCGCCAGC ATGACTGACG ACAGGGACAG AGGAAGTCGG 
GATAGAGGAA GTCGGGACAG GGGAGGCAGA GACCAAGGAA GCAGAGACCG AGAAAGCCGG 
GACAGGGGAA GCAGAGACCA AGGTAGCAGA GACAGAGGAA GTCGGGACAG GGTTCCGAGC 
AAAGATCTCA CAGCTAAGCG TGAGAGCACC CCCACTCCTC CTCCTTCTCT CTCTAAACCT 
GCTCTGACTG AGGAGGAGGT GGAGAAGAAG TCAAGCGCCA TCATTGAAGA ATACCTCCAC 
ATCAATGACT TGAAGGAGGC GCTGCAGTGT GTGACAGAGC TCAACAGTGC CTCGCTGCTG 
TACGTGTTTG TGCGGAACGG CTTGGAGTCG ACGCTGGAGC GCAGCACAAT CGCTAGAGAG 
CGCATGGGCC ACTTGCTGCA CCAACTTATA AAGGCAGGGA CATTGCCCAC ACTGCAGTAC 
TACAAAGGGC TAGAGGAGAT CCTGGAGGTA GCCGAAGACA TGGCCATAGA TATACCTCAC 
ATATGGCTCT ACCTGGCTGA ACTCATTACC CCCATGCTCC ATGAGGGAGG CATCCCTATG 
GGACAGCTCT TCAGGGAGAT CTCAAAGCCT CTCCTGCCTC TGGGGAAGGC CGCTGTGCTG 
CTGGTCCAGA TCCTTAAGCT GCTCTGCAAC GGAATGACCC CTAAGAAGGT CGGGGCCATG 
TGGACAGAGG ACGGGCTGAG CTGGAGTGAC TTCTTACCCA AGGACGAGGA CGTCAACAAG 
TTTGTCACTG AACAGCAGGT GGATTTCACC ACAGGAGAAA CGACGGAGTC ACTGGAAGTG 
GTTCTTAAGA GAGCCCTGAG TGGAGAAGAG CTCAGTCAAC AGCTGGACAG ACTCCTCGAG 
GATAAGGCCA ACAACCAGCG CATCGAAGAC TGGGTTGAGG CTAACTTAGA GGAGCAGCAG 
ATGACTTCCA ACCAGTTCGT AAGAGCATTG ATGACAGCAG TGTGCCAGTC CGCCATCATA 
TGTGACAACC CGTACAGGGT GGATCCAAAT CAGATCAAAG AGAGAGCCAG ACTTCTGCAG 
AAATACATGT CTGACGAGCA GAAAGAGCTC CAGGCCCTTT ACGCCCTCCA GGCACTGATG 
GTGCATATGG AGCAGCCTGC GAACCTGCTG CGGATGTTCT TCGACGCCTT GTACGATGAG 
GACGTCATTA AAGAAGAGGC CTTCTATAAG TGGGAATCAA GCAAAGACCC TGCAGAGCAG 
ACGGGCAAAG GTGTGGCCTT GAAGTCTGTC ACCGCCTTCT TCACCTGGCT CCGCGAGGCA 
GAGGAGGAGG AGTCCGACAA GGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019959166.1
CDS	509..5635
Translation	MNKPPQPITGPTSVPNPAPSPGLTQAAYGPGQPPSLVFATPPPPQMNSAPQTRQTYYPNRPAMATNAPRVQTSSGPRPVGPTHVYPPSSQMMMISQQQLSFAGSPQGYFIPPPGQYRAPYMPPTQQYPVTSGTAAFYPGTSPAEYPAYAGAYYPAQPQYPPSVQPAPVMINPTQQQPQAPPPQQPPAQAQGPLKRERKPIRIRDPNQGGRDITEEIMSGGRSTTTPTPPQASIADLSPAQTNGEVVQPVATVTRQDDNMEPAVSAETPPPPATADTEPVLEAKQEVDSQMLVCAESDVPSVAPTAATEDPSPLINDEQSPSSLPPAVAATTTTTPAEAENNDVTKVSDTVDAPVGPSASIATQEAPVKIEEPQAAPAPPEKAPEQEEKITEEVKTVEKEEQVASSKLEPVVEDAPIVTPVEVAEEEAVTKTSTEMSHPPPSKQELVAPQTQSPAPNSGPEPEPTLAETAEPLVSNGLPQNIEELSEDVTLSDTTSLDKPDASQSQESTPVAKMAALTQEEEEEEEIEVEKEEIKGEEKEKIEDVPPTPISCPTEESTTQAATSLPKKRRNMKELNKKEAIGDLLDAFTDEQEAKPASEPLPTPADPVPVAPAEPPAEAADETWEEKEDKQNAEPDRPKASPEPTEQKYQYKEEQWKPINPEEKKQYDRDFLLGFQFISASMNKPEGLPTISDVVLDKVNKTPLRPADPARLINVGPDFTPSYLGNLGSRSVGGPRGPPPGPRRSQQGQRKEPRKIIISSMSLNDDVQLNKAEKAWKPSVKKSTRSRCAEETDTDDSEQGKTKELFKRLRSILNKLTPQKFQELMKQVTDLAIDTEERLKGAIDLIFEKAISEPNFSVAYANMCRCLMGLKVPTTDKPGVSANFRKLLLNRCQKEFEKDQDDDEIFEKKQKELEASKDDEERERLRVELNDSRDQARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLFSTIGKDLDFEKAKPRMDQYFNQMDKIIKERKTSSRIRFMLQDVLDLRKCNWVPRRGDQGPKTIDQIHKEAEMEEHREHIKVQQLMSKKEAGGGRMGGGGGGMGGRGSHTPGGGRNNPPQDEGWNTVPISKNRPIDTTRLSKITKPGALDFSNQLLAPGGKGMWGSWGKGSSGGTGAKPASGEQDAGRPATSTLNRFSALQSGSLSSSDSDRRAPQRSSSSRERGGDRGDREKDRFDRFDRNEGREGRDDRSTRNQITKRSFSRESQERGGRVGDTRTPTEPVRRVASMTDDRDRGSRDRGSRDRGGRDQGSRDRESRDRGSRDQGSRDRGSRDRVPSKDLTAKRESTPTPPPSLSKPALTEEEVEKKSSAIIEEYLHINDLKEALQCVTELNSASLLYVFVRNGLESTLERSTIARERMGHLLHQLIKAGTLPTLQYYKGLEEILEVAEDMAIDIPHIWLYLAELITPMLHEGGIPMGQLFREISKPLLPLGKAAVLLVQILKLLCNGMTPKKVGAMWTEDGLSWSDFLPKDEDVNKFVTEQQVDFTTGETTESLEVVLKRALSGEELSQQLDRLLEDKANNQRIEDWVEANLEEQQMTSNQFVRALMTAVCQSAIICDNPYRVDPNQIKERARLLQKYMSDEQKELQALYALQALMVHMEQPANLLRMFFDALYDEDVIKEEAFYKWESSKDPAEQTGKGVALKSVTAFFTWLREAEEEESDKE

Target ORF information:

RefSeq Version	XM_020103607.1
Organism	Paralichthys olivaceus(Japanese flounder)
Definition	Paralichthys olivaceus eukaryotic translation initiation factor 4 gamma 1-like (LOC109639892), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020103607.1

ORF Insert Sequence:

ATGAACAAGC CACCACAGCC TATAACGGGA CCCACCTCTG TCCCAAACCC TGCCCCTTCC 
CCTGGATTGA CACAGGCTGC CTACGGCCCA GGACAGCCCC CTTCTCTTGT TTTTGCCACC 
CCTCCACCTC CACAAATGAA CTCTGCACCC CAGACAAGAC AGACTTACTA TCCAAACCGA 
CCAGCCATGG CAACCAATGC TCCAAGAGTA CAGACAAGTA GTGGGCCCAG ACCTGTTGGA 
CCTACCCATG TCTACCCGCC CAGCTCCCAG ATGATGATGA TTTCCCAGCA GCAGCTGTCT 
TTTGCTGGAT CCCCTCAGGG CTACTTCATC CCCCCTCCTG GACAGTACCG GGCCCCATAC 
ATGCCTCCAA CTCAGCAGTA TCCTGTGACC AGCGGTACAG CTGCCTTCTA TCCAGGAACC 
AGCCCTGCTG AATACCCCGC TTATGCAGGA GCATATTATC CAGCACAGCC ACAGTACCCT 
CCATCGGTCC AGCCTGCACC GGTCATGATC AACCCCACCC AGCAACAGCC ACAAGCCCCG 
CCTCCTCAGC AACCACCGGC ACAGGCACAA GGCCCACTAA AGAGAGAACG CAAACCGATA 
AGAATACGAG ACCCCAACCA GGGCGGGCGT GATATCACAG AAGAGATAAT GTCAGGTGGA 
AGATCCACCA CCACACCGAC TCCCCCACAG GCCTCCATAG CAGATTTAAG TCCTGCACAG 
ACCAACGGTG AAGTTGTACA GCCTGTCGCT ACAGTGACGA GACAAGATGA TAACATGGAG 
CCTGCTGTAA GCGCTGAAAC CCCGCCGCCT CCTGCCACAG CAGACACAGA GCCTGTGCTG 
GAGGCCAAAC AGGAAGTGGA CAGCCAGATG TTGGTGTGTG CTGAATCAGA TGTACCATCT 
GTAGCCCCCA CAGCTGCTAC TGAGGATCCA TCCCCATTGA TAAATGATGA GCAGTCTCCC 
TCTTCCCTCC CTCCTGCAGT AGCAGCTACT ACTACTACTA CTCCTGCCGA GGCAGAAAAT 
AATGACGTTA CTAAAGTCAG TGACACAGTA GATGCTCCTG TCGGCCCTTC GGCATCAATA 
GCAACACAAG AGGCACCTGT TAAAATAGAG GAACCACAGG CTGCCCCAGC TCCACCTGAG 
AAGGCACCAG AACAGGAAGA AAAGATAACA GAGGAAGTGA AGACAGTTGA AAAAGAAGAG 
CAGGTGGCTA GTTCCAAGTT GGAGCCTGTA GTTGAAGATG CACCAATAGT TACCCCTGTT 
GAAGTGGCAG AAGAAGAAGC AGTGACTAAG ACGTCAACTG AAATGTCTCA TCCTCCACCC 
TCCAAACAGG AACTAGTTGC TCCACAGACC CAGAGCCCTG CTCCGAACTC TGGCCCTGAA 
CCCGAACCCA CTCTGGCTGA AACAGCAGAG CCTCTCGTCT CCAACGGTCT TCCTCAGAAC 
ATTGAGGAAC TTTCTGAGGA TGTTACGCTT TCAGACACTA CATCCCTTGA CAAGCCAGAT 
GCCTCTCAAT CTCAGGAATC CACACCTGTA GCTAAAATGG CAGCACTAAC CCAGGAAGAG 
GAGGAAGAGG AGGAGATTGA GGTGGAGAAG GAGGAGATTA AAGGGGAAGA GAAGGAGAAA 
ATTGAGGATG TCCCTCCTAC CCCTATCAGC TGCCCCACAG AGGAATCTAC TACGCAAGCT 
GCTACGTCTC TGCCAAAGAA GAGGAGGAAC ATGAAGGAGC TCAACAAAAA GGAGGCCATT 
GGAGACCTCC TGGATGCATT CACAGATGAG CAGGAGGCCA AGCCGGCTTC TGAACCTTTG 
CCCACTCCGG CCGACCCTGT CCCTGTTGCT CCAGCTGAAC CTCCTGCTGA GGCTGCAGAT 
GAGACTTGGG AAGAGAAAGA GGACAAGCAG AACGCAGAAC CTGACAGGCC CAAAGCCTCG 
CCTGAGCCAA CTGAGCAGAA ATACCAGTAC AAAGAAGAAC AATGGAAGCC GATAAACCCA 
GAGGAGAAGA AGCAGTACGA CAGGGATTTC CTCCTGGGCT TCCAGTTCAT CAGCGCCAGT 
ATGAACAAAC CCGAGGGTCT GCCCACTATC AGTGATGTGG TCCTGGACAA GGTGAACAAG 
ACTCCTCTGC GACCTGCTGA CCCAGCTCGA CTAATTAACG TTGGTCCTGA TTTTACTCCC 
TCCTATTTGG GTAATCTTGG GAGCAGATCA GTGGGAGGAC CAAGAGGCCC ACCTCCGGGG 
CCACGTCGCT CCCAGCAGGG TCAGCGTAAA GAACCTAGGA AAATCATCAT CAGCAGCATG 
TCCCTCAATG ATGACGTGCA ACTCAACAAG GCTGAGAAGG CCTGGAAGCC CTCAGTGAAG 
AAGTCCACTC GCAGCCGCTG TGCTGAGGAA ACCGATACAG ATGACAGTGA ACAGGGCAAG 
ACTAAAGAGC TGTTCAAGCG TCTGCGCAGT ATCCTCAACA AGTTGACTCC TCAGAAGTTT 
CAGGAGCTGA TGAAGCAGGT GACAGATCTG GCGATAGATA CGGAGGAGAG GCTGAAGGGA 
GCCATTGACC TCATCTTTGA GAAGGCCATC TCAGAGCCCA ACTTCTCTGT GGCCTACGCC 
AATATGTGCC GCTGCCTTAT GGGATTGAAA GTCCCAACTA CGGACAAGCC AGGAGTTTCT 
GCCAACTTCC GTAAACTGCT GCTTAACCGC TGTCAGAAAG AGTTTGAGAA GGACCAGGAT 
GATGATGAGA TCTTTGAGAA GAAGCAAAAA GAGTTGGAGG CTTCCAAAGA TGATGAAGAA 
CGTGAACGCT TGAGGGTGGA GCTGAATGAT TCCAGAGACC AGGCCCGCCG GCGCTCACTG 
GGAAACATAA AGTTCATAGG CGAGCTCTTC AAGCTGAAGA TGCTGACTGA GGCCATCATG 
CACGACTGCG TAGTGAAACT ACTAAAGAAT CATGATGAAG AGTCTCTGGA GTGTCTCTGC 
AGGCTGTTCT CCACTATTGG AAAAGATCTG GACTTTGAAA AGGCCAAGCC TCGTATGGAT 
CAGTATTTTA ACCAGATGGA CAAGATCATC AAAGAGAGAA AGACTTCATC CAGAATCCGC 
TTCATGCTTC AAGACGTCCT GGACCTCAGA AAGTGCAACT GGGTGCCTCG CAGAGGAGAC 
CAGGGTCCTA AAACAATTGA CCAAATCCAC AAGGAGGCAG AGATGGAGGA GCACAGGGAG 
CACATCAAAG TGCAGCAGCT CATGTCCAAG AAAGAGGCAG GTGGAGGTAG GATGGGAGGA 
GGAGGAGGGG GCATGGGGGG CCGAGGGTCT CACACACCAG GAGGTGGCCG GAATAACCCG 
CCTCAGGATG AGGGATGGAA CACGGTGCCC ATCTCCAAGA ACAGACCCAT CGACACCACC 
CGCCTTAGCA AGATCACCAA GCCCGGTGCT CTGGACTTCA GCAACCAACT GTTGGCTCCA 
GGTGGCAAAG GCATGTGGGG CAGCTGGGGC AAAGGCAGCA GTGGAGGAAC TGGAGCTAAA 
CCCGCCAGTG GAGAGCAAGA TGCTGGTCGT CCAGCTACTA GCACCCTCAA CCGATTCTCT 
GCCCTGCAGT CTGGTTCTCT GTCTTCATCA GACTCCGATC GCAGAGCTCC TCAGAGGTCA 
AGCTCCAGTC GCGAGCGCGG TGGAGACAGG GGCGACCGCG AGAAGGATCG CTTTGACCGA 
TTTGATCGCA ATGAGGGACG GGAAGGTCGT GACGACAGGA GCACCCGCAA CCAAATCACG 
AAGAGAAGCT TCAGCAGAGA GTCGCAGGAG CGCGGAGGGA GGGTTGGAGA CACCCGGACC 
CCGACTGAGC CTGTGCGTCG CGTCGCCAGC ATGACTGACG ACAGGGACAG AGGAAGTCGG 
GATAGAGGAA GTCGGGACAG GGGAGGCAGA GACCAAGGAA GCAGAGACCG AGAAAGCCGG 
GACAGGGGAA GCAGAGACCA AGGTAGCAGA GACAGAGGAA GTCGGGACAG GGTTCCGAGC 
AAAGATCTCA CAGCTAAGCG TGAGAGCACC CCCACTCCTC CTCCTTCTCT CTCTAAACCT 
GCTCTGACTG AGGAGGAGGT GGAGAAGAAG TCAAGCGCCA TCATTGAAGA ATACCTCCAC 
ATCAATGACT TGAAGGAGGC GCTGCAGTGT GTGACAGAGC TCAACAGTGC CTCGCTGCTG 
TACGTGTTTG TGCGGAACGG CTTGGAGTCG ACGCTGGAGC GCAGCACAAT CGCTAGAGAG 
CGCATGGGCC ACTTGCTGCA CCAACTTATA AAGGCAGGGA CATTGCCCAC ACTGCAGTAC 
TACAAAGGGC TAGAGGAGAT CCTGGAGGTA GCCGAAGACA TGGCCATAGA TATACCTCAC 
ATATGGCTCT ACCTGGCTGA ACTCATTACC CCCATGCTCC ATGAGGGAGG CATCCCTATG 
GGACAGCTCT TCAGGGAGAT CTCAAAGCCT CTCCTGCCTC TGGGGAAGGC CGCTGTGCTG 
CTGGTCCAGA TCCTTAAGCT GCTCTGCAAC GGAATGACCC CTAAGAAGGT CGGGGCCATG 
TGGACAGAGG ACGGGCTGAG CTGGAGTGAC TTCTTACCCA AGGACGAGGA CGTCAACAAG 
TTTGTCACTG AACAGCAGGT GGATTTCACC ACAGGAGAAA CGACGGAGTC ACTGGAAGTG 
GTTCTTAAGA GAGCCCTGAG TGGAGAAGAG CTCAGTCAAC AGCTGGACAG ACTCCTCGAG 
GATAAGGCCA ACAACCAGCG CATCGAAGAC TGGGTTGAGG CTAACTTAGA GGAGCAGCAG 
ATGACTTCCA ACCAGTTCGT AAGAGCATTG ATGACAGCAG TGTGCCAGTC CGCCATCATA 
TGTGACAACC CGTACAGGGT GGATCCAAAT CAGATCAAAG AGAGAGCCAG ACTTCTGCAG 
AAATACATGT CTGACGAGCA GAAAGAGCTC CAGGCCCTTT ACGCCCTCCA GGCACTGATG 
GTGCATATGG AGCAGCCTGC GAACCTGCTG CGGATGTTCT TCGACGCCTT GTACGATGAG 
GACGTCATTA AAGAAGAGGC CTTCTATAAG TGGGAATCAA GCAAAGACCC TGCAGAGCAG 
ACGGGCAAAG GTGTGGCCTT GAAGTCTGTC ACCGCCTTCT TCACCTGGCT CCGCGAGGCA 
GAGGAGGAGG AGTCCGACAA GGAATAA