fam13a cDNA ORF clone, Paralichthys olivaceus(Japanese flounder)

Gene
Clones
gRNAs

Summary

Gene Symbol	fam13a
Entrez Gene ID	109638357
Full Name	family with sequence similarity 13 member A
Gene Type	protein-coding
Organism	Paralichthys olivaceus(Japanese flounder)

mRNA and Protein(s)

mRNA	Protein	Name
XM_020101305.1	XP_019956864.1	protein FAM13A isoform X2
XM_020101304.1	XP_019956863.1	protein FAM13A isoform X1

Pathways from BioSystems
Gene Ontology

The following fam13a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the fam13a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPa251619	XM_020101304.1 Latest version!	Paralichthys olivaceus family with sequence similarity 13 member A (fam13a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OPa251620	XM_020101305.1 Latest version!	Paralichthys olivaceus family with sequence similarity 13 member A (fam13a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

fam13a ( XM_020101304.1 ) cDNA ORF clone, Paralichthys olivaceus(Japanese flounder) -> XP_019956863.1 Paralichthys olivaceus family with sequence similarity 13 member A (fam13a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPa251619
Clone ID Related Accession (Same CDS sequence)	XM_020101304.1
Accession Version	XM_020101304.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3315bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-07
Organism	Paralichthys olivaceus(Japanese flounder)
Product	protein FAM13A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017859681.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Paralichthys olivaceus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGCAG GAGCTCTGAC CATCTGTCAC AACAAGACGG CAGTGCAGGT TAAAGAAGAC 
ATGAAGAAGA TGGTGCAGAT ACCCATGCTG AGACCCAGGA TGGTGGGAGC AAAGCACACC 
AAGCTGTTTG GAGCGTCCCT GTTTGAGCTG CGTCAGAGGG GCCTGGTGGA GAACGGTGTG 
CCTCTGGTGG TGCAGAGGAT GGTGGAGCAT CTCCGCAATC ATGCCCTGCA TCAGGAAGGC 
CTGTTCAGAG TGAATGGGAA CGTCCGGGCT GTGGAGACCC TGAAACAGCG GCTGGAGGGA 
GGCGAGGAAG TGGACCTTCT CTCTGAATCT GACACGTGTA CAGTGGCTAG TTTGCTGAAA 
CGGTATCTCA GGGAGCTGCC AGACGGTCTG GTAGACTCAA CAGTCCAGCA GGCTCTAATA 
CAGCACTTCC AAGAGTGCGG TGATGATGGC TCTTGGTCAG ATATGAGAGA CCTGCTCCAG 
CAGCTTCCAG ATGTTCACCA CAGCCTCCTC CGCTACCTCT GCCACTTCCT TACGCTCGTC 
GAGTGCAACC ACAAAGACAA CCGCATGACT GCCCTCAACC TCGCCACCGT GTTTGGACCC 
AGCGTCTTCC ATGTGGCTCC TGGGTTTGAA GCAATGAAGG ACCAAAATAT CTGTAACAAG 
CTCATGGTCA AGCTCATACA GAACTACAGC AACATTTTTG AGGAAGACAA AGAAAAAGAA 
AGCTGCTCAG AAGAACTATC TACCCTAATC ATTGTCAAGG AGGCGTGCGT GATGGACACA 
AACACAAAGT CACCCAGCCA CCTCAATGCG AAGACACCAA CACCAATTCC CAGGACAAAA 
GTTGGACAAA AAGACGTTCC AGAAAACGTA CCCGGACCTT TGATCAAATC CACCTCTAAC 
ACTGCAAAGG CAAGATCAAG GAGGAAGAAG GTGAAGAAAG GGAAGAGTGA CAGCATGCCT 
GAAGTTGTCC TTCAGCCCAG CCGCTCTGCA CACTTGCCTC ATGTGAGACC TGGCTCCATT 
CCAATAGTTT TGTCCCTAAG TTCTGAACTG AGAAGCCCAA GTCCAGAGGC GATGGAGACA 
TCTCCCATAA CTCTGGCTCA CCGAGACACC CCAGACCCTG CCTCGCACCC CAGCCATCTT 
GATGTCAGCC CCTCTGGTAG CATGGAAGCC CTCAGTAGTT CCCAAGAAGA AGAGAGGCCA 
ATATCTCCTT TCTACATGAG TAACCACCTC TCCCCTGTCC ACTGCAGACC AGATGTGGTG 
AATTTCCTGG ACAAGACCAT ACGATCAACA GTAGAGCAGC ACCTCTTTGA CGTGAACCCT 
TTGCGGGATC AAAGTTCAAA GGAGTGTGAA TTGACATCTC CAAAAGTGAC GCCAACTGCT 
CGACAGAGAC GTCGACACCA GAGAGAGCAG CAGGAGGAAG GACTCAGACA ACGAGAGAGA 
AACGGAGCTG CATCCATCAC AGCAGACACC AACAAGGAGA ACATCCCATC GAGCTGTCGA 
AGCAGTTCGG GCAGCGTGGG GGAGGACGCA GGCGGTGGCA GCAGCAACGT GGACGCACAG 
GGAGGCCAGA CCGCTCACAC TGAGCGAACC CCCAAACCCA GGAAGTCAAA ACACAGTCCC 
ACGCTGGTGC TACTCACTGA CAATCAGGAT GCACACACAG TAAAGGAGTG TGTTGATGAG 
AGACCTGGAC CAAAGCCCAT GGAGACATCT CAGACAGGGA ATGGCATTTC AAGAAAAAAT 
GGTCTTCCTT CTCCTCCACA GACGTTAAGG ATGAAGATCC AGGAGTCGCC TGTCACTTGT 
GACACGGGGA GGGTCAGCAG CGGAGAGGTG CGGGACTCGG ACTGTGAGAA GACGAGCTGC 
GAGGATGTTC CCCGGCTGGA CCTCACAGCA CTGACCGAGG ACGACAACTG GGGAGAATCT 
TCATTCTTTA ACATCCCTTT CCTTGATTTC AAAAGCATTT TCCATCCAAG TTTTTGTGAT 
GAACCCGTCC CAGCGTTCTC ATCACTGCAG AGGGAGAGCA TGGACCGCGA GGAAGCTCGA 
CTGTCCCCTC ACGCGGGGGG GCGACCGATC CGCCAGCTGC TGGAGGAAGA CAGCGACCCC 
ATGCTGTCAC CTCGCTTCTA CACCTACGGA CAGTGCCAGC AGTACCTGGA CGACACTGAA 
GTGCCTCCCT CACCGCCAAA CGCACACTCG TTTGTCAGTC GCAGACGGAG TTCATCTCTC 
GGCTCCTGTG ACGACGAGAA AGAGGAGTTG ACATCGGCTC AGCTCACAAA GAGGGTTCAC 
TTCCTGAAGA AGAAGATCCA CAGATTTGAG GAGAAGTTTG AAGATGAGCG AAAATACAGG 
CCTTCCCATA GTGATAAAGC TGCAAACCCA GAAGTGCTGA GGTGGGTAAC TGAACTGGCC 
AAACTCCGCA AAGAGCTCAA AGAACACAAG CTGATGAAGT CTGAGGAGGA TCTTCCACCA 
CTGACCCGTC AGCGCAGCAA CACTTTGCCT AAAAGCTTCG GCTCTCAGCT GGAGAAGAAA 
CCGCAGCAGG AGAAAGTGCC GAAGCCGCCG GTGGAGAGCA CGCTGGAGAC CATTTTAAAG 
AAGCTGCAGG AGAAGAGGGA GGAGGTGAAC CGCCCCGAGG ACATCAAGGA TATGACACGG 
GAGCAGATCG GAGCAGAGAA AGTCGCCCTG CAGAAAGCTC TCCTCTACTA CGAGAGCATA 
CACGGACGAC CAGTCACCAA AAGTGAGAGG CAAATCATGA AGCCGCTCTA CGACAGATAC 
CGCCTGGTCA AACAGATCCT GTGCAGAGCC TCCACCATCC CTGTCATTGG CTCCCCCTCC 
AGCAAGCGCA GAGGGCCGCT GCTTCAGCCC ATTATCGAGG GCGAAACCGC GCTCTTCTTC 
GATGACATCA AGGAGGAGGA GGACGGCTCA GAGGACGACG GAGACTCCAA GACTCAGTTC 
ACCGTGACCG AGCGGCCCGA CCTCAGCGTC CTCGGCTTCC TCGACCACAT GGAGGAGGAA 
GCGGACGGTT TCATTTCGCC AGTGGATGAG CTCTCGCCGT CTCAAAACAC AACAGACATG 
AGGCTGTCGA ACCTTCACTC CGCCACCATG CCGGAGCTGG TGGAACAGCT CCAAGAGTCC 
AGAGAGGAGA AGAAGAGGAT CCGCAAAAAC CTGAAAGAGT TCGAGGACCA GTTCTTCAGA 
CAGAACGGAA GAAATGTGCA GAAGGAGGAC CGCTCCCCAT TGGCAGTGGA GTACAACGAA 
TACAAGCATG TTAAAGCCAA ACTGCGGCTG CTGGAAGTCC TCATCAGCAA AAGAGAGTCG 
TCCAAGTTCA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019956863.1
CDS	290..3604
Translation	MGAGALTICHNKTAVQVKEDMKKMVQIPMLRPRMVGAKHTKLFGASLFELRQRGLVENGVPLVVQRMVEHLRNHALHQEGLFRVNGNVRAVETLKQRLEGGEEVDLLSESDTCTVASLLKRYLRELPDGLVDSTVQQALIQHFQECGDDGSWSDMRDLLQQLPDVHHSLLRYLCHFLTLVECNHKDNRMTALNLATVFGPSVFHVAPGFEAMKDQNICNKLMVKLIQNYSNIFEEDKEKESCSEELSTLIIVKEACVMDTNTKSPSHLNAKTPTPIPRTKVGQKDVPENVPGPLIKSTSNTAKARSRRKKVKKGKSDSMPEVVLQPSRSAHLPHVRPGSIPIVLSLSSELRSPSPEAMETSPITLAHRDTPDPASHPSHLDVSPSGSMEALSSSQEEERPISPFYMSNHLSPVHCRPDVVNFLDKTIRSTVEQHLFDVNPLRDQSSKECELTSPKVTPTARQRRRHQREQQEEGLRQRERNGAASITADTNKENIPSSCRSSSGSVGEDAGGGSSNVDAQGGQTAHTERTPKPRKSKHSPTLVLLTDNQDAHTVKECVDERPGPKPMETSQTGNGISRKNGLPSPPQTLRMKIQESPVTCDTGRVSSGEVRDSDCEKTSCEDVPRLDLTALTEDDNWGESSFFNIPFLDFKSIFHPSFCDEPVPAFSSLQRESMDREEARLSPHAGGRPIRQLLEEDSDPMLSPRFYTYGQCQQYLDDTEVPPSPPNAHSFVSRRRSSSLGSCDDEKEELTSAQLTKRVHFLKKKIHRFEEKFEDERKYRPSHSDKAANPEVLRWVTELAKLRKELKEHKLMKSEEDLPPLTRQRSNTLPKSFGSQLEKKPQQEKVPKPPVESTLETILKKLQEKREEVNRPEDIKDMTREQIGAEKVALQKALLYYESIHGRPVTKSERQIMKPLYDRYRLVKQILCRASTIPVIGSPSSKRRGPLLQPIIEGETALFFDDIKEEEDGSEDDGDSKTQFTVTERPDLSVLGFLDHMEEEADGFISPVDELSPSQNTTDMRLSNLHSATMPELVEQLQESREEKKRIRKNLKEFEDQFFRQNGRNVQKEDRSPLAVEYNEYKHVKAKLRLLEVLISKRESSKFI

Target ORF information:

RefSeq Version	XM_020101304.1
Organism	Paralichthys olivaceus(Japanese flounder)
Definition	Paralichthys olivaceus family with sequence similarity 13 member A (fam13a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020101304.1

ORF Insert Sequence:

ATGGGGGCAG GAGCTCTGAC CATCTGTCAC AACAAGACGG CAGTGCAGGT TAAAGAAGAC 
ATGAAGAAGA TGGTGCAGAT ACCCATGCTG AGACCCAGGA TGGTGGGAGC AAAGCACACC 
AAGCTGTTTG GAGCGTCCCT GTTTGAGCTG CGTCAGAGGG GCCTGGTGGA GAACGGTGTG 
CCTCTGGTGG TGCAGAGGAT GGTGGAGCAT CTCCGCAATC ATGCCCTGCA TCAGGAAGGC 
CTGTTCAGAG TGAATGGGAA CGTCCGGGCT GTGGAGACCC TGAAACAGCG GCTGGAGGGA 
GGCGAGGAAG TGGACCTTCT CTCTGAATCT GACACGTGTA CAGTGGCTAG TTTGCTGAAA 
CGGTATCTCA GGGAGCTGCC AGACGGTCTG GTAGACTCAA CAGTCCAGCA GGCTCTAATA 
CAGCACTTCC AAGAGTGCGG TGATGATGGC TCTTGGTCAG ATATGAGAGA CCTGCTCCAG 
CAGCTTCCAG ATGTTCACCA CAGCCTCCTC CGCTACCTCT GCCACTTCCT TACGCTCGTC 
GAGTGCAACC ACAAAGACAA CCGCATGACT GCCCTCAACC TCGCCACCGT GTTTGGACCC 
AGCGTCTTCC ATGTGGCTCC TGGGTTTGAA GCAATGAAGG ACCAAAATAT CTGTAACAAG 
CTCATGGTCA AGCTCATACA GAACTACAGC AACATTTTTG AGGAAGACAA AGAAAAAGAA 
AGCTGCTCAG AAGAACTATC TACCCTAATC ATTGTCAAGG AGGCGTGCGT GATGGACACA 
AACACAAAGT CACCCAGCCA CCTCAATGCG AAGACACCAA CACCAATTCC CAGGACAAAA 
GTTGGACAAA AAGACGTTCC AGAAAACGTA CCCGGACCTT TGATCAAATC CACCTCTAAC 
ACTGCAAAGG CAAGATCAAG GAGGAAGAAG GTGAAGAAAG GGAAGAGTGA CAGCATGCCT 
GAAGTTGTCC TTCAGCCCAG CCGCTCTGCA CACTTGCCTC ATGTGAGACC TGGCTCCATT 
CCAATAGTTT TGTCCCTAAG TTCTGAACTG AGAAGCCCAA GTCCAGAGGC GATGGAGACA 
TCTCCCATAA CTCTGGCTCA CCGAGACACC CCAGACCCTG CCTCGCACCC CAGCCATCTT 
GATGTCAGCC CCTCTGGTAG CATGGAAGCC CTCAGTAGTT CCCAAGAAGA AGAGAGGCCA 
ATATCTCCTT TCTACATGAG TAACCACCTC TCCCCTGTCC ACTGCAGACC AGATGTGGTG 
AATTTCCTGG ACAAGACCAT ACGATCAACA GTAGAGCAGC ACCTCTTTGA CGTGAACCCT 
TTGCGGGATC AAAGTTCAAA GGAGTGTGAA TTGACATCTC CAAAAGTGAC GCCAACTGCT 
CGACAGAGAC GTCGACACCA GAGAGAGCAG CAGGAGGAAG GACTCAGACA ACGAGAGAGA 
AACGGAGCTG CATCCATCAC AGCAGACACC AACAAGGAGA ACATCCCATC GAGCTGTCGA 
AGCAGTTCGG GCAGCGTGGG GGAGGACGCA GGCGGTGGCA GCAGCAACGT GGACGCACAG 
GGAGGCCAGA CCGCTCACAC TGAGCGAACC CCCAAACCCA GGAAGTCAAA ACACAGTCCC 
ACGCTGGTGC TACTCACTGA CAATCAGGAT GCACACACAG TAAAGGAGTG TGTTGATGAG 
AGACCTGGAC CAAAGCCCAT GGAGACATCT CAGACAGGGA ATGGCATTTC AAGAAAAAAT 
GGTCTTCCTT CTCCTCCACA GACGTTAAGG ATGAAGATCC AGGAGTCGCC TGTCACTTGT 
GACACGGGGA GGGTCAGCAG CGGAGAGGTG CGGGACTCGG ACTGTGAGAA GACGAGCTGC 
GAGGATGTTC CCCGGCTGGA CCTCACAGCA CTGACCGAGG ACGACAACTG GGGAGAATCT 
TCATTCTTTA ACATCCCTTT CCTTGATTTC AAAAGCATTT TCCATCCAAG TTTTTGTGAT 
GAACCCGTCC CAGCGTTCTC ATCACTGCAG AGGGAGAGCA TGGACCGCGA GGAAGCTCGA 
CTGTCCCCTC ACGCGGGGGG GCGACCGATC CGCCAGCTGC TGGAGGAAGA CAGCGACCCC 
ATGCTGTCAC CTCGCTTCTA CACCTACGGA CAGTGCCAGC AGTACCTGGA CGACACTGAA 
GTGCCTCCCT CACCGCCAAA CGCACACTCG TTTGTCAGTC GCAGACGGAG TTCATCTCTC 
GGCTCCTGTG ACGACGAGAA AGAGGAGTTG ACATCGGCTC AGCTCACAAA GAGGGTTCAC 
TTCCTGAAGA AGAAGATCCA CAGATTTGAG GAGAAGTTTG AAGATGAGCG AAAATACAGG 
CCTTCCCATA GTGATAAAGC TGCAAACCCA GAAGTGCTGA GGTGGGTAAC TGAACTGGCC 
AAACTCCGCA AAGAGCTCAA AGAACACAAG CTGATGAAGT CTGAGGAGGA TCTTCCACCA 
CTGACCCGTC AGCGCAGCAA CACTTTGCCT AAAAGCTTCG GCTCTCAGCT GGAGAAGAAA 
CCGCAGCAGG AGAAAGTGCC GAAGCCGCCG GTGGAGAGCA CGCTGGAGAC CATTTTAAAG 
AAGCTGCAGG AGAAGAGGGA GGAGGTGAAC CGCCCCGAGG ACATCAAGGA TATGACACGG 
GAGCAGATCG GAGCAGAGAA AGTCGCCCTG CAGAAAGCTC TCCTCTACTA CGAGAGCATA 
CACGGACGAC CAGTCACCAA AAGTGAGAGG CAAATCATGA AGCCGCTCTA CGACAGATAC 
CGCCTGGTCA AACAGATCCT GTGCAGAGCC TCCACCATCC CTGTCATTGG CTCCCCCTCC 
AGCAAGCGCA GAGGGCCGCT GCTTCAGCCC ATTATCGAGG GCGAAACCGC GCTCTTCTTC 
GATGACATCA AGGAGGAGGA GGACGGCTCA GAGGACGACG GAGACTCCAA GACTCAGTTC 
ACCGTGACCG AGCGGCCCGA CCTCAGCGTC CTCGGCTTCC TCGACCACAT GGAGGAGGAA 
GCGGACGGTT TCATTTCGCC AGTGGATGAG CTCTCGCCGT CTCAAAACAC AACAGACATG 
AGGCTGTCGA ACCTTCACTC CGCCACCATG CCGGAGCTGG TGGAACAGCT CCAAGAGTCC 
AGAGAGGAGA AGAAGAGGAT CCGCAAAAAC CTGAAAGAGT TCGAGGACCA GTTCTTCAGA 
CAGAACGGAA GAAATGTGCA GAAGGAGGAC CGCTCCCCAT TGGCAGTGGA GTACAACGAA 
TACAAGCATG TTAAAGCCAA ACTGCGGCTG CTGGAAGTCC TCATCAGCAA AAGAGAGTCG 
TCCAAGTTCA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fam13a ( XM_020101305.1 ) cDNA ORF clone, Paralichthys olivaceus(Japanese flounder) -> XP_019956864.1 Paralichthys olivaceus family with sequence similarity 13 member A (fam13a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPa251620
Clone ID Related Accession (Same CDS sequence)	XM_020101305.1
Accession Version	XM_020101305.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3165bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-07
Organism	Paralichthys olivaceus(Japanese flounder)
Product	protein FAM13A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017859681.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Paralichthys olivaceus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGCAG GAGCTCTGAC CATCTGTCAC AACAAGACGG CAGTGCAGGT TAAAGAAGAC 
ATGAAGAAGA TGGTGCAGAT ACCCATGCTG AGACCCAGGA TGGTGGGAGC AAAGCACACC 
AAGCTGTTTG GAGCGTCCCT GTTTGAGCTG CGTCAGAGGG GCCTGGTGGA GAACGGTGTG 
CCTCTGGTGG TGCAGAGGAT GGTGGAGCAT CTCCGCAATC ATGCCCTGCA TCAGGAAGGC 
CTGTTCAGAG TGAATGGGAA CGTCCGGGCT GTGGAGACCC TGAAACAGCG GCTGGAGGGA 
GGCGAGGAAG TGGACCTTCT CTCTGAATCT GACACGTGTA CAGTGGCTAG TTTGCTGAAA 
CGGTATCTCA GGGAGCTGCC AGACGGTCTG GTAGACTCAA CAGTCCAGCA GGCTCTAATA 
CAGCACTTCC AAGAGTGCGG TGATGATGGC TCTTGGTCAG ATATGAGAGA CCTGCTCCAG 
CAGCTTCCAG ATGTTCACCA CAGCCTCCTC CGCTACCTCT GCCACTTCCT TACGCTCGTC 
GAGTGCAACC ACAAAGACAA CCGCATGACT GCCCTCAACC TCGCCACCGT GTTTGGACCC 
AGCGTCTTCC ATGTGGCTCC TGGGTTTGAA GCAATGAAGG ACCAAAATAT CTGTAACAAG 
CTCATGGTCA AGCTCATACA GAACTACAGC AACATTTTTG AGGAAGACAA AGAAAAAGAA 
AGCTGCTCAG AAGAACTATC TACCCTAATC ATTGTCAAGG AGGCGTGCGT GATGGACACA 
AACACAAAGT CACCCAGCCA CCTCAATGCG AAGACACCAA CACCAATTCC CAGGACAAAA 
GTTGGACAAA AAGACGTTCC AGAAAACGTA CCCGGACCTT TGATCAAATC CACCTCTAAC 
ACTGCAAAGG CAAGATCAAG GAGGAAGAAG GTGAAGAAAG GGAAGAGTGA CAGCATGCCT 
GAAGTTGTCC TTCAGCCCAG CCGCTCTGCA CACTTGCCTC ATGTGAGACC TGGCTCCATT 
CCAATAGTTT TGTCCCTAAG TTCTGAACTG AGAAGCCCAA GTCCAGAGGC GATGGAGACA 
TCTCCCATAA CTCTGGCTCA CCGAGACACC CCAGACCCTG CCTCGCACCC CAGCCATCTT 
GATGTCAGCC CCTCTGGTAG CATGGAAGCC CTCAGTAGTT CCCAAGAAGA AGAGAGGCCA 
ATATCTCCTT TCTACATGAG TAACCACCTC TCCCCTGTCC ACTGCAGACC AGATGTGGTG 
AATTTCCTGG ACAAGACCAT ACGATCAACA GTAGAGCAGC ACCTCTTTGA CGTGAACCCT 
TTGCGGGATC AAAGTTCAAA GGAGTGTGAA TTGACATCTC CAAAAGTGAC GCCAACTGCT 
CGACAGAGAC GTCGACACCA GAGAGAGCAG CAGGAGGAAG GACTCAGACA ACGAGAGAGA 
AACGGAGCTG CATCCATCAC AGCAGACACC AACAAGGAGA ACATCCCATC GAGCTGTCGA 
AGCAGTTCGG GCAGCGTGGG GGAGGACGCA GGCGGTGGCA GCAGCAACGT GGACGCACAG 
GGAGGCCAGA CCGCTCACAC TGAGCGAACC CCCAAACCCA GGAAGTCAAA ACACAGTCCC 
ACGCTGGTGC TACTCACTGA CAATCAGGAT GCACACACAG TAAAGGAGTG TGTTGATGAG 
AGACCTGGAC CAAAGCCCAT GGAGACATCT CAGACAGGGA ATGGCATTTC AAGAAAAAAT 
GGTCTTCCTT CTCCTCCACA GACGTTAAGG ATGAAGATCC AGGAGTCGCC TGTCACTTGT 
GACACGGGGA GGGTCAGCAG CGGAGAGGTG CGGGACTCGG ACTGTGAGAA GACGAGCTGC 
GAGGATGTTC CCCGGCTGGA CCTCACAGCA CTGACCGAGG ACGACAACTG GGGAGAACCC 
GTCCCAGCGT TCTCATCACT GCAGAGGGAG AGCATGGACC GCGAGGAAGC TCGACTGTCC 
CCTCACGCGG GGGGGCGACC GATCCGCCAG CTGCTGGAGG AAGACAGCGA CCCCATGCTG 
TCACCTCGCT TCTACACCTA CGGACAGTGC CAGCAGTACC TGGACGACAC TGAAGTGCCT 
CCCTCACCGC CAAACGCACA CTCGTTTGTC AGTCGCAGAC GGAGTTCATC TCTCGGCTCC 
TGTGACGACG AGAAAGAGGA GTTGACATCG GCTCAGCTCA CAAAGAGGGT TCACTTCCTG 
AAGAAGAAGA TCCACAGATT TGAGGAGAAG TTTGAAGATG AGCGAAAATA CAGGCCTTCC 
CATAGTGATA AAGCTGCAAA CCCAGAAGTG CTGAGGTGGG TAACTGAACT GGCCAAACTC 
CGCAAAGAGC TCAAAGAACA CAAGCTGATG AAGTCTGAGG AGGATCTTCC ACCACTGACC 
CGTCAGCGCA GCAACACTTT GCCTAAAAGC TTCGGCTCTC AGCTGGAGAA GAAACCGCAG 
CAGGAGAAAG TGCCGAAGCC GCCGGTGGAG AGCACGCTGG AGACCATTTT AAAGAAGCTG 
CAGGAGAAGA GGGAGGAGGT GAACCGCCCC GAGGACATCA AGGATATGAC ACGGGAGCAG 
ATCGGAGCAG AGAAAGTCGC CCTGCAGAAA GCTCTCCTCT ACTACGAGAG CATACACGGA 
CGACCAGTCA CCAAAAGTGA GAGGCAAATC ATGAAGCCGC TCTACGACAG ATACCGCCTG 
GTCAAACAGA TCCTGTGCAG AGCCTCCACC ATCCCTGTCA TTGAGGAGGA GGACGGCTCA 
GAGGACGACG GAGACTCCAA GACTCAGTTC ACCGTGACCG AGCGGCCCGA CCTCAGCGTC 
CTCGGCTTCC TCGACCACAT GGAGGAGGAA GCGGACGGTT TCATTTCGCC AGTGGATGAG 
CTCTCGCCGT CTCAAAACAC AACAGACATG AGGCTGTCGA ACCTTCACTC CGCCACCATG 
CCGGAGCTGG TGGAACAGCT CCAAGAGTCC AGAGAGGAGA AGAAGAGGAT CCGCAAAAAC 
CTGAAAGAGT TCGAGGACCA GTTCTTCAGA CAGAACGGAA GAAATGTGCA GAAGGAGGAC 
CGCTCCCCAT TGGCAGTGGA GTACAACGAA TACAAGCATG TTAAAGCCAA ACTGCGGCTG 
CTGGAAGTCC TCATCAGCAA AAGAGAGTCG TCCAAGTTCA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019956864.1
CDS	286..3450
Translation	MGAGALTICHNKTAVQVKEDMKKMVQIPMLRPRMVGAKHTKLFGASLFELRQRGLVENGVPLVVQRMVEHLRNHALHQEGLFRVNGNVRAVETLKQRLEGGEEVDLLSESDTCTVASLLKRYLRELPDGLVDSTVQQALIQHFQECGDDGSWSDMRDLLQQLPDVHHSLLRYLCHFLTLVECNHKDNRMTALNLATVFGPSVFHVAPGFEAMKDQNICNKLMVKLIQNYSNIFEEDKEKESCSEELSTLIIVKEACVMDTNTKSPSHLNAKTPTPIPRTKVGQKDVPENVPGPLIKSTSNTAKARSRRKKVKKGKSDSMPEVVLQPSRSAHLPHVRPGSIPIVLSLSSELRSPSPEAMETSPITLAHRDTPDPASHPSHLDVSPSGSMEALSSSQEEERPISPFYMSNHLSPVHCRPDVVNFLDKTIRSTVEQHLFDVNPLRDQSSKECELTSPKVTPTARQRRRHQREQQEEGLRQRERNGAASITADTNKENIPSSCRSSSGSVGEDAGGGSSNVDAQGGQTAHTERTPKPRKSKHSPTLVLLTDNQDAHTVKECVDERPGPKPMETSQTGNGISRKNGLPSPPQTLRMKIQESPVTCDTGRVSSGEVRDSDCEKTSCEDVPRLDLTALTEDDNWGEPVPAFSSLQRESMDREEARLSPHAGGRPIRQLLEEDSDPMLSPRFYTYGQCQQYLDDTEVPPSPPNAHSFVSRRRSSSLGSCDDEKEELTSAQLTKRVHFLKKKIHRFEEKFEDERKYRPSHSDKAANPEVLRWVTELAKLRKELKEHKLMKSEEDLPPLTRQRSNTLPKSFGSQLEKKPQQEKVPKPPVESTLETILKKLQEKREEVNRPEDIKDMTREQIGAEKVALQKALLYYESIHGRPVTKSERQIMKPLYDRYRLVKQILCRASTIPVIEEEDGSEDDGDSKTQFTVTERPDLSVLGFLDHMEEEADGFISPVDELSPSQNTTDMRLSNLHSATMPELVEQLQESREEKKRIRKNLKEFEDQFFRQNGRNVQKEDRSPLAVEYNEYKHVKAKLRLLEVLISKRESSKFI

Target ORF information:

RefSeq Version	XM_020101305.1
Organism	Paralichthys olivaceus(Japanese flounder)
Definition	Paralichthys olivaceus family with sequence similarity 13 member A (fam13a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020101305.1

ORF Insert Sequence:

ATGGGGGCAG GAGCTCTGAC CATCTGTCAC AACAAGACGG CAGTGCAGGT TAAAGAAGAC 
ATGAAGAAGA TGGTGCAGAT ACCCATGCTG AGACCCAGGA TGGTGGGAGC AAAGCACACC 
AAGCTGTTTG GAGCGTCCCT GTTTGAGCTG CGTCAGAGGG GCCTGGTGGA GAACGGTGTG 
CCTCTGGTGG TGCAGAGGAT GGTGGAGCAT CTCCGCAATC ATGCCCTGCA TCAGGAAGGC 
CTGTTCAGAG TGAATGGGAA CGTCCGGGCT GTGGAGACCC TGAAACAGCG GCTGGAGGGA 
GGCGAGGAAG TGGACCTTCT CTCTGAATCT GACACGTGTA CAGTGGCTAG TTTGCTGAAA 
CGGTATCTCA GGGAGCTGCC AGACGGTCTG GTAGACTCAA CAGTCCAGCA GGCTCTAATA 
CAGCACTTCC AAGAGTGCGG TGATGATGGC TCTTGGTCAG ATATGAGAGA CCTGCTCCAG 
CAGCTTCCAG ATGTTCACCA CAGCCTCCTC CGCTACCTCT GCCACTTCCT TACGCTCGTC 
GAGTGCAACC ACAAAGACAA CCGCATGACT GCCCTCAACC TCGCCACCGT GTTTGGACCC 
AGCGTCTTCC ATGTGGCTCC TGGGTTTGAA GCAATGAAGG ACCAAAATAT CTGTAACAAG 
CTCATGGTCA AGCTCATACA GAACTACAGC AACATTTTTG AGGAAGACAA AGAAAAAGAA 
AGCTGCTCAG AAGAACTATC TACCCTAATC ATTGTCAAGG AGGCGTGCGT GATGGACACA 
AACACAAAGT CACCCAGCCA CCTCAATGCG AAGACACCAA CACCAATTCC CAGGACAAAA 
GTTGGACAAA AAGACGTTCC AGAAAACGTA CCCGGACCTT TGATCAAATC CACCTCTAAC 
ACTGCAAAGG CAAGATCAAG GAGGAAGAAG GTGAAGAAAG GGAAGAGTGA CAGCATGCCT 
GAAGTTGTCC TTCAGCCCAG CCGCTCTGCA CACTTGCCTC ATGTGAGACC TGGCTCCATT 
CCAATAGTTT TGTCCCTAAG TTCTGAACTG AGAAGCCCAA GTCCAGAGGC GATGGAGACA 
TCTCCCATAA CTCTGGCTCA CCGAGACACC CCAGACCCTG CCTCGCACCC CAGCCATCTT 
GATGTCAGCC CCTCTGGTAG CATGGAAGCC CTCAGTAGTT CCCAAGAAGA AGAGAGGCCA 
ATATCTCCTT TCTACATGAG TAACCACCTC TCCCCTGTCC ACTGCAGACC AGATGTGGTG 
AATTTCCTGG ACAAGACCAT ACGATCAACA GTAGAGCAGC ACCTCTTTGA CGTGAACCCT 
TTGCGGGATC AAAGTTCAAA GGAGTGTGAA TTGACATCTC CAAAAGTGAC GCCAACTGCT 
CGACAGAGAC GTCGACACCA GAGAGAGCAG CAGGAGGAAG GACTCAGACA ACGAGAGAGA 
AACGGAGCTG CATCCATCAC AGCAGACACC AACAAGGAGA ACATCCCATC GAGCTGTCGA 
AGCAGTTCGG GCAGCGTGGG GGAGGACGCA GGCGGTGGCA GCAGCAACGT GGACGCACAG 
GGAGGCCAGA CCGCTCACAC TGAGCGAACC CCCAAACCCA GGAAGTCAAA ACACAGTCCC 
ACGCTGGTGC TACTCACTGA CAATCAGGAT GCACACACAG TAAAGGAGTG TGTTGATGAG 
AGACCTGGAC CAAAGCCCAT GGAGACATCT CAGACAGGGA ATGGCATTTC AAGAAAAAAT 
GGTCTTCCTT CTCCTCCACA GACGTTAAGG ATGAAGATCC AGGAGTCGCC TGTCACTTGT 
GACACGGGGA GGGTCAGCAG CGGAGAGGTG CGGGACTCGG ACTGTGAGAA GACGAGCTGC 
GAGGATGTTC CCCGGCTGGA CCTCACAGCA CTGACCGAGG ACGACAACTG GGGAGAACCC 
GTCCCAGCGT TCTCATCACT GCAGAGGGAG AGCATGGACC GCGAGGAAGC TCGACTGTCC 
CCTCACGCGG GGGGGCGACC GATCCGCCAG CTGCTGGAGG AAGACAGCGA CCCCATGCTG 
TCACCTCGCT TCTACACCTA CGGACAGTGC CAGCAGTACC TGGACGACAC TGAAGTGCCT 
CCCTCACCGC CAAACGCACA CTCGTTTGTC AGTCGCAGAC GGAGTTCATC TCTCGGCTCC 
TGTGACGACG AGAAAGAGGA GTTGACATCG GCTCAGCTCA CAAAGAGGGT TCACTTCCTG 
AAGAAGAAGA TCCACAGATT TGAGGAGAAG TTTGAAGATG AGCGAAAATA CAGGCCTTCC 
CATAGTGATA AAGCTGCAAA CCCAGAAGTG CTGAGGTGGG TAACTGAACT GGCCAAACTC 
CGCAAAGAGC TCAAAGAACA CAAGCTGATG AAGTCTGAGG AGGATCTTCC ACCACTGACC 
CGTCAGCGCA GCAACACTTT GCCTAAAAGC TTCGGCTCTC AGCTGGAGAA GAAACCGCAG 
CAGGAGAAAG TGCCGAAGCC GCCGGTGGAG AGCACGCTGG AGACCATTTT AAAGAAGCTG 
CAGGAGAAGA GGGAGGAGGT GAACCGCCCC GAGGACATCA AGGATATGAC ACGGGAGCAG 
ATCGGAGCAG AGAAAGTCGC CCTGCAGAAA GCTCTCCTCT ACTACGAGAG CATACACGGA 
CGACCAGTCA CCAAAAGTGA GAGGCAAATC ATGAAGCCGC TCTACGACAG ATACCGCCTG 
GTCAAACAGA TCCTGTGCAG AGCCTCCACC ATCCCTGTCA TTGAGGAGGA GGACGGCTCA 
GAGGACGACG GAGACTCCAA GACTCAGTTC ACCGTGACCG AGCGGCCCGA CCTCAGCGTC 
CTCGGCTTCC TCGACCACAT GGAGGAGGAA GCGGACGGTT TCATTTCGCC AGTGGATGAG 
CTCTCGCCGT CTCAAAACAC AACAGACATG AGGCTGTCGA ACCTTCACTC CGCCACCATG 
CCGGAGCTGG TGGAACAGCT CCAAGAGTCC AGAGAGGAGA AGAAGAGGAT CCGCAAAAAC 
CTGAAAGAGT TCGAGGACCA GTTCTTCAGA CAGAACGGAA GAAATGTGCA GAAGGAGGAC 
CGCTCCCCAT TGGCAGTGGA GTACAACGAA TACAAGCATG TTAAAGCCAA ACTGCGGCTG 
CTGGAAGTCC TCATCAGCAA AAGAGAGTCG TCCAAGTTCA TCTGA