slc12a1 cDNA ORF clone, Paralichthys olivaceus(Japanese flounder)

Gene
Clones
gRNAs

Summary

Gene Symbol	slc12a1
Entrez Gene ID	109637281
Full Name	solute carrier family 12 member 1
Synonyms	fNKCC
Gene Type	protein-coding
Organism	Paralichthys olivaceus(Japanese flounder)

mRNA and Protein(s)

mRNA	Protein	Name
XM_020099529.1	XP_019955088.1	solute carrier family 12 member 1

Pathways from BioSystems
Gene Ontology

The following slc12a1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the slc12a1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPa250195	XM_020099529.1 Latest version!	Paralichthys olivaceus solute carrier family 12 member 1 (slc12a1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

slc12a1 ( XM_020099529.1 ) cDNA ORF clone, Paralichthys olivaceus(Japanese flounder) -> XP_019955088.1 Paralichthys olivaceus solute carrier family 12 member 1 (slc12a1), mRNA.

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CloneID	OPa250195
Clone ID Related Accession (Same CDS sequence)	XM_020099529.1
Accession Version	XM_020099529.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3132bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-07
Organism	Paralichthys olivaceus(Japanese flounder)
Product	solute carrier family 12 member 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017859674.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Paralichthys olivaceus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGGT TCAAGTCTAA CGGAGGGGAG CATGTGAACC CCGCGTATGA TTCCAACCTG 
GATGAGCCAC CTGTCTATCA GGACCCCAAC TTCTCCAACG GGGAACAACG GACGGTGCGG 
CCCTCGGTGG TCAGTGCCTT TGGTCACGAC ACTTTGGACC GAGTGCCCAA CATTGACTTC 
TATCGCAATG CAGGCAGTGT CAGCGGTCAC CGTGCCGTGC GGCCATCCCT GCAGGAGCTC 
CATGATGTGT TTCAGAAGAA TGGAGGGATC TCTGTTCCAG ACACCGTGGA GGACGATGGC 
GAGGGAAGTG ACGGGACACC CTCTGATGAC CTGGAGTCTG CCGAACCCCC CAATGAAAAC 
AAAGGAGGAG TAAAGTTTGG CTGGATAAGA GGCGTCCTGG TGAGATGCAT GCTGAACATC 
TGGGGTGTCA TGTTGTTCAT CCGTCTGTCC TGGGTTTTCG GTCAGGCAGG CTGGGGCCTG 
GGAATCGTGG TCATCGCTCT CAGCTGCGTG GTCACCGTCA TCACTGGCCT CTCCATGTCG 
GCCATTTGTA CTAATGGTGT AGTCAGAGGA GGAGGCGCCT ACTACTTGAT ATCTCGCAGT 
CTTGGGCCTG AGTTCGGCGG ATCCATCGGC CTCATCTTCG CCTTCGCCAA TGCTGTGGCC 
GTGGCCATGT ACGTGGTGGG GTTTGCTGAG ACCGTGGTCG ACTTGCTGGA GGAGAACTCT 
GCCATCATGG TCGATGAAAT AAACGACATC AGGATCGTCG GCTGTATCAC GGTGGTGCTG 
CTGTTGGCCA TATCGGTTGC TGGGATGGAA TGGGAGGCCA AGGCGCAGAT CGTGCTCCTC 
GTCATCCTGC TGGTTGCCAT AGTGAACGTG TTTGTAGGAA CTTTCATCCC TGCCACCGCT 
GAGAAGAAAT CCAAAGGCTT CTTCAATTAT GAAATGAGAA TCTTCCAAGA AAATTTTACT 
CCAAACTTCA GTGAGAATGA GTCGTTTTTC ACGGTGTTTT CCATCTTTTT CCCGGCCGCC 
ACGGGGATCC TGGCCGGAGC CAACATCTCC GGCGACTTGC GGGATCCCCA AGCAGCCATA 
CCTAAGGGAA CCTTGCTTGC CATCGTGATC ACTGGCATCA CCTACTTGGC TGTGGCCCTT 
TCTGTCTCTG CCTCTGTTGT CCGAGATGCC ACAGGAAACA TAACTGACCT CTTAATTCCC 
GGGGGAAATT GTAGCCATTC TGCGTTTGTT AGTTGTGAGC TCGGCTATAA TTTCTCCTCC 
TGCGCAGAGC AAACCTGTCG GTTTGGCTTG ATGAACGACT TCCAGGTGAT GACCATGGTG 
TCTGGGTTCG GTCCCCTCAT CATTGCTGGA ACTTTCTCGG CGACCCTCTC CTCTGCCCTT 
GCTTCCCTTG TCAGCGCTCC CAAAGTCTTC CAGGCTCTGT GTAAAGACAA CATTTACAAG 
GCTCTGCATT TCTTTGCCAA AGGACACGGC AAGAACAACG AGCCAATCCG AGGCTACATC 
CTCACATTCA TTATTTCCGT GGCCTTCATT CTCATTGGTA ACCTCAACAC CATCGCTCCA 
ATAATTTCCA ACTTCTTCCT GGCATCTTAC GCTCTCATCA ATTTCTCCTG CTTCCACGCA 
TCCTACGCCA AGTCTCCAGG CTGGAGACCT GGATATAAGT ACTACAACAT GTGGCTGTCC 
CTGGTGGGGG CAGTGCTCTG CTGCGCCGTC ATGTTTGTCA TCAACTGGTG GGCTGCTCTC 
CTCACGTACG CTATCGAGAT CCTCCTCTAC ATTTACGTCA CCGTCAAGAA GCCAGATGTG 
AACTGGGGTT CGTCCACGCA GGCGGTGACG TTTGTGAGTG CGGTCAGCAA CGCCCTGTCT 
CTGTCTGGTG TCGAGGATCA CGTCAAGAAC TTCAGGCCTC AGATTTTGGC TCTGACCGGT 
TCAGCGCGGT CCAGACCGGC TCTCCTCGAC CTGGCTCACT CCTTCTCCAA GAACTATGGT 
CTCTGCATCA CCTGTGAAGT CTTTGAGGGT CCAAGATCAG AGGCTCTGGA GGAGATGAAC 
GCCGGCATGG AGAAGAACCA GCTCTGGCTC AGGAAGAACA AACGCAAGGC GTTCTATGCA 
GCTGTGGCCT GTGAAAGCTT CAGGGAAGGA ACAGAAAGCC TCCTGCAGGC CTCTGGTCTC 
GGCCGCATGA AGCCCAACAC ACTGATGATC GGCTTCAAGA GAAACTGGAG GATGGCCGAT 
GCAGAGTCAG TGCAGAGCTA TGTGGGAATC CTGCATGACG CGTTTGACTT TGAGTACGGG 
ACATTGGTGC TGAGGATGAA CCAGGGACTT GATGTGTCAC ACATTGTGGA AGCAGAAGAG 
GAGATGCTGA AGGCAGCGAA GGAGCAACAG GCCCTGGAGG ACGAAATGAT GCCAAACGGA 
GGGAAATCAA AGGGACTGTT TAGGAAGTCC AGGAAATCGT CTCAACAAGT GCTCACAACC 
AGAGTGTCGG TGTGCGGACC TCCACCCCCG CAGGTTGCCA GGATGAATGA GAGGCTGTTA 
GAGGCCAGTG CTCAATTCAA GAAGAAGCAG CCTAAAGGCA CGATCGATGT GTGGTGGCTC 
TTCGACGATG GAGGCCTCAC CCTGCTGCTC CCCTACATCC TCACCACCAG GAAGAAGTGG 
AAAGACTCTA AATTAAGGAT CTTCATCGCA GGGCAGCCTG ACCGCAGCGA GCTGGATAAA 
CAGGAGATGA AGTCTCTGCT GCAGAAATTC AGGATAAACT GCACTGACAT CAAAGTCATT 
GATGACATCC ACGTCAAACC CAGCTCCGCC AGCCTGAAGC AGTTAGAGGA CATGATCGAG 
CCTTTCCGTC TGCGTGAGGG CTCCAAAGAC AAAATCCAAG CTGAAGCCAT GCAGAGAGAG 
CAGCCCTGGA AAATCACTGA CGAGGAGCTG AACAGCTTCG AGGAGAAGAC CAACCTCCAG 
GTGCGACTGA ACGAGGTGCT CCAGGAGAAC TCCAAATCAG CCAACCTGAT CATCGTGAGC 
ATGCCCATTG CTCGCAAGGA GTCCGTGTCT GACTTCCTCT ACATGGCCTG GCTGGACATT 
CTAACCAAGG ATCTTCCACC AACGCTGCTC ATCAGAGGAA ACCACAAGAG CGTTCTTACT 
TTCTACTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019955088.1
CDS	173..3304
Translation	MERFKSNGGEHVNPAYDSNLDEPPVYQDPNFSNGEQRTVRPSVVSAFGHDTLDRVPNIDFYRNAGSVSGHRAVRPSLQELHDVFQKNGGISVPDTVEDDGEGSDGTPSDDLESAEPPNENKGGVKFGWIRGVLVRCMLNIWGVMLFIRLSWVFGQAGWGLGIVVIALSCVVTVITGLSMSAICTNGVVRGGGAYYLISRSLGPEFGGSIGLIFAFANAVAVAMYVVGFAETVVDLLEENSAIMVDEINDIRIVGCITVVLLLAISVAGMEWEAKAQIVLLVILLVAIVNVFVGTFIPATAEKKSKGFFNYEMRIFQENFTPNFSENESFFTVFSIFFPAATGILAGANISGDLRDPQAAIPKGTLLAIVITGITYLAVALSVSASVVRDATGNITDLLIPGGNCSHSAFVSCELGYNFSSCAEQTCRFGLMNDFQVMTMVSGFGPLIIAGTFSATLSSALASLVSAPKVFQALCKDNIYKALHFFAKGHGKNNEPIRGYILTFIISVAFILIGNLNTIAPIISNFFLASYALINFSCFHASYAKSPGWRPGYKYYNMWLSLVGAVLCCAVMFVINWWAALLTYAIEILLYIYVTVKKPDVNWGSSTQAVTFVSAVSNALSLSGVEDHVKNFRPQILALTGSARSRPALLDLAHSFSKNYGLCITCEVFEGPRSEALEEMNAGMEKNQLWLRKNKRKAFYAAVACESFREGTESLLQASGLGRMKPNTLMIGFKRNWRMADAESVQSYVGILHDAFDFEYGTLVLRMNQGLDVSHIVEAEEEMLKAAKEQQALEDEMMPNGGKSKGLFRKSRKSSQQVLTTRVSVCGPPPPQVARMNERLLEASAQFKKKQPKGTIDVWWLFDDGGLTLLLPYILTTRKKWKDSKLRIFIAGQPDRSELDKQEMKSLLQKFRINCTDIKVIDDIHVKPSSASLKQLEDMIEPFRLREGSKDKIQAEAMQREQPWKITDEELNSFEEKTNLQVRLNEVLQENSKSANLIIVSMPIARKESVSDFLYMAWLDILTKDLPPTLLIRGNHKSVLTFYS

Target ORF information:

RefSeq Version	XM_020099529.1
Organism	Paralichthys olivaceus(Japanese flounder)
Definition	Paralichthys olivaceus solute carrier family 12 member 1 (slc12a1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020099529.1

ORF Insert Sequence:

ATGGAGAGGT TCAAGTCTAA CGGAGGGGAG CATGTGAACC CCGCGTATGA TTCCAACCTG 
GATGAGCCAC CTGTCTATCA GGACCCCAAC TTCTCCAACG GGGAACAACG GACGGTGCGG 
CCCTCGGTGG TCAGTGCCTT TGGTCACGAC ACTTTGGACC GAGTGCCCAA CATTGACTTC 
TATCGCAATG CAGGCAGTGT CAGCGGTCAC CGTGCCGTGC GGCCATCCCT GCAGGAGCTC 
CATGATGTGT TTCAGAAGAA TGGAGGGATC TCTGTTCCAG ACACCGTGGA GGACGATGGC 
GAGGGAAGTG ACGGGACACC CTCTGATGAC CTGGAGTCTG CCGAACCCCC CAATGAAAAC 
AAAGGAGGAG TAAAGTTTGG CTGGATAAGA GGCGTCCTGG TGAGATGCAT GCTGAACATC 
TGGGGTGTCA TGTTGTTCAT CCGTCTGTCC TGGGTTTTCG GTCAGGCAGG CTGGGGCCTG 
GGAATCGTGG TCATCGCTCT CAGCTGCGTG GTCACCGTCA TCACTGGCCT CTCCATGTCG 
GCCATTTGTA CTAATGGTGT AGTCAGAGGA GGAGGCGCCT ACTACTTGAT ATCTCGCAGT 
CTTGGGCCTG AGTTCGGCGG ATCCATCGGC CTCATCTTCG CCTTCGCCAA TGCTGTGGCC 
GTGGCCATGT ACGTGGTGGG GTTTGCTGAG ACCGTGGTCG ACTTGCTGGA GGAGAACTCT 
GCCATCATGG TCGATGAAAT AAACGACATC AGGATCGTCG GCTGTATCAC GGTGGTGCTG 
CTGTTGGCCA TATCGGTTGC TGGGATGGAA TGGGAGGCCA AGGCGCAGAT CGTGCTCCTC 
GTCATCCTGC TGGTTGCCAT AGTGAACGTG TTTGTAGGAA CTTTCATCCC TGCCACCGCT 
GAGAAGAAAT CCAAAGGCTT CTTCAATTAT GAAATGAGAA TCTTCCAAGA AAATTTTACT 
CCAAACTTCA GTGAGAATGA GTCGTTTTTC ACGGTGTTTT CCATCTTTTT CCCGGCCGCC 
ACGGGGATCC TGGCCGGAGC CAACATCTCC GGCGACTTGC GGGATCCCCA AGCAGCCATA 
CCTAAGGGAA CCTTGCTTGC CATCGTGATC ACTGGCATCA CCTACTTGGC TGTGGCCCTT 
TCTGTCTCTG CCTCTGTTGT CCGAGATGCC ACAGGAAACA TAACTGACCT CTTAATTCCC 
GGGGGAAATT GTAGCCATTC TGCGTTTGTT AGTTGTGAGC TCGGCTATAA TTTCTCCTCC 
TGCGCAGAGC AAACCTGTCG GTTTGGCTTG ATGAACGACT TCCAGGTGAT GACCATGGTG 
TCTGGGTTCG GTCCCCTCAT CATTGCTGGA ACTTTCTCGG CGACCCTCTC CTCTGCCCTT 
GCTTCCCTTG TCAGCGCTCC CAAAGTCTTC CAGGCTCTGT GTAAAGACAA CATTTACAAG 
GCTCTGCATT TCTTTGCCAA AGGACACGGC AAGAACAACG AGCCAATCCG AGGCTACATC 
CTCACATTCA TTATTTCCGT GGCCTTCATT CTCATTGGTA ACCTCAACAC CATCGCTCCA 
ATAATTTCCA ACTTCTTCCT GGCATCTTAC GCTCTCATCA ATTTCTCCTG CTTCCACGCA 
TCCTACGCCA AGTCTCCAGG CTGGAGACCT GGATATAAGT ACTACAACAT GTGGCTGTCC 
CTGGTGGGGG CAGTGCTCTG CTGCGCCGTC ATGTTTGTCA TCAACTGGTG GGCTGCTCTC 
CTCACGTACG CTATCGAGAT CCTCCTCTAC ATTTACGTCA CCGTCAAGAA GCCAGATGTG 
AACTGGGGTT CGTCCACGCA GGCGGTGACG TTTGTGAGTG CGGTCAGCAA CGCCCTGTCT 
CTGTCTGGTG TCGAGGATCA CGTCAAGAAC TTCAGGCCTC AGATTTTGGC TCTGACCGGT 
TCAGCGCGGT CCAGACCGGC TCTCCTCGAC CTGGCTCACT CCTTCTCCAA GAACTATGGT 
CTCTGCATCA CCTGTGAAGT CTTTGAGGGT CCAAGATCAG AGGCTCTGGA GGAGATGAAC 
GCCGGCATGG AGAAGAACCA GCTCTGGCTC AGGAAGAACA AACGCAAGGC GTTCTATGCA 
GCTGTGGCCT GTGAAAGCTT CAGGGAAGGA ACAGAAAGCC TCCTGCAGGC CTCTGGTCTC 
GGCCGCATGA AGCCCAACAC ACTGATGATC GGCTTCAAGA GAAACTGGAG GATGGCCGAT 
GCAGAGTCAG TGCAGAGCTA TGTGGGAATC CTGCATGACG CGTTTGACTT TGAGTACGGG 
ACATTGGTGC TGAGGATGAA CCAGGGACTT GATGTGTCAC ACATTGTGGA AGCAGAAGAG 
GAGATGCTGA AGGCAGCGAA GGAGCAACAG GCCCTGGAGG ACGAAATGAT GCCAAACGGA 
GGGAAATCAA AGGGACTGTT TAGGAAGTCC AGGAAATCGT CTCAACAAGT GCTCACAACC 
AGAGTGTCGG TGTGCGGACC TCCACCCCCG CAGGTTGCCA GGATGAATGA GAGGCTGTTA 
GAGGCCAGTG CTCAATTCAA GAAGAAGCAG CCTAAAGGCA CGATCGATGT GTGGTGGCTC 
TTCGACGATG GAGGCCTCAC CCTGCTGCTC CCCTACATCC TCACCACCAG GAAGAAGTGG 
AAAGACTCTA AATTAAGGAT CTTCATCGCA GGGCAGCCTG ACCGCAGCGA GCTGGATAAA 
CAGGAGATGA AGTCTCTGCT GCAGAAATTC AGGATAAACT GCACTGACAT CAAAGTCATT 
GATGACATCC ACGTCAAACC CAGCTCCGCC AGCCTGAAGC AGTTAGAGGA CATGATCGAG 
CCTTTCCGTC TGCGTGAGGG CTCCAAAGAC AAAATCCAAG CTGAAGCCAT GCAGAGAGAG 
CAGCCCTGGA AAATCACTGA CGAGGAGCTG AACAGCTTCG AGGAGAAGAC CAACCTCCAG 
GTGCGACTGA ACGAGGTGCT CCAGGAGAAC TCCAAATCAG CCAACCTGAT CATCGTGAGC 
ATGCCCATTG CTCGCAAGGA GTCCGTGTCT GACTTCCTCT ACATGGCCTG GCTGGACATT 
CTAACCAAGG ATCTTCCACC AACGCTGCTC ATCAGAGGAA ACCACAAGAG CGTTCTTACT 
TTCTACTCCT GA