nolc1 cDNA ORF clone, Paralichthys olivaceus(Japanese flounder)

Gene
Clones
gRNAs

Summary

Gene Symbol	nolc1
Entrez Gene ID	109634709
Full Name	nucleolar and coiled-body phosphoprotein 1
Gene Type	protein-coding
Organism	Paralichthys olivaceus(Japanese flounder)

mRNA and Protein(s)

mRNA	Protein	Name
XM_020095403.1	XP_019950962.1	nucleolar and coiled-body phosphoprotein 1 isoform X1
XM_020095409.1	XP_019950968.1	nucleolar and coiled-body phosphoprotein 1 isoform X2

Pathways from BioSystems
Gene Ontology

The following nolc1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nolc1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPa231944	XM_020095403.1 Latest version!	Paralichthys olivaceus nucleolar and coiled-body phosphoprotein 1 (nolc1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OPa231945	XM_020095409.1 Latest version!	Paralichthys olivaceus nucleolar and coiled-body phosphoprotein 1 (nolc1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nolc1 ( XM_020095403.1 ) cDNA ORF clone, Paralichthys olivaceus(Japanese flounder) -> XP_019950962.1 Paralichthys olivaceus nucleolar and coiled-body phosphoprotein 1 (nolc1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPa231944
Clone ID Related Accession (Same CDS sequence)	XM_020095403.1
Accession Version	XM_020095403.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3072bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-07
Organism	Paralichthys olivaceus(Japanese flounder)
Product	nucleolar and coiled-body phosphoprotein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017859642.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Paralichthys olivaceus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCCGGA GCTCCGCACA CTTCTTTCCT TCCAGAAAGA GCGTGGTAAA GATGGCGGAG 
AAGAGCTCGG TGCCGAGTGA TCTCTACAAG TGCGTTTATT CATTTCTACG GGAAAACAAG 
TTCACCAGGG CGGCGCAGCA GTTCCTGAAG CAGACCCAAG TGACTCCCCA AGACGAAAAT 
GAGGAAAACC TCGTCAACAT CTACAACTTC TGGGTGAAGT CTCCTGAATC CAAGAAACGA 
AAAGCGCCTC CTACCAAGGC TGAAGCTGGC GACGGTCCAT CGTCCAAGAA AGCAAAGCCA 
AGTACAGAGT CCTCCAGCAG CGAAGAGTCG AGCAGCGAGG ATGAAGAAGC TGCTGCAAAA 
CCAGCTAAGG CAGCCGCTGC AGCAGCTAAA GTGGTCCCTG CTAAAGCAGC AGCTGCTAAA 
GCAGCAGCTG CTAAAGCTGC ATCCAGCAGC AGTGAAGACT CCAGTGACTC TGAGGAGGAG 
AAGGCTCCTG TAAAGGCTCC TGTGAAGAAG GCTGCTGCTG TGAAGAAGGT TGTTGCTGCT 
CCTGCTGCGA AGAAGGTTGT TGCTGCTCCT GCTGCAGGTG GCACAAGGAA GAAAGACAGC 
AGCTCAAGCA GCGAAGAGTC CGACTCTGAG GAGGAGGAGC CGGCTAAAGC CCCTGCACCA 
AAACCCAAGC CGGGTGCCGT CACTACATCT AAACCTGCTG CTCCTGCTAA AGGTGCAGGT 
CAGAAAAAAC AGGAGAGCAC CAGTGAAGAC AGTTCCTCAG ATTCTGAAGC TGAAGAACCA 
GCAAAGGCTC CAGCCAAACC CACCCCAGTA AAAGCTGCTG CTGCAGCCGA ATCAACCAGT 
GAAGACTCTT CATCCGAAGA TGAGGCTCCT CCCAGTAAAA AACCCAAAGC AGGAGCATAC 
AGCGCCGTAC CACCTCCTGC ATCAGTCCAG AAAGCACCAG CCGCACCGGC TGCCAGCAAG 
GCCAAGGACA GCGACTCCTC AGACAGCAGT GATGACAGCA GTGATGAGGA AGAAGAGAAG 
AAAGTGGCTG CAAAACCTGC CCCAGTTAAG ACCCCTGCTG TCAAACCTGC CGCGGCCAAA 
CCTGCTGCAA AGAAGCAGGA ATCCAGCTCA GAGAGCTCAG ATTCAAGCTC AGAAGAAGAG 
GCAAAGAAGA CCCCAGCCAA AGTCACCCCA GCTAAAGCAG CCCCAGCCAA AGCAGCCCCA 
GCTAAAGCAG CCCCAGCTAA AGCAGCCCCA GCTAAAGCAG CCCCAGCCAA AGCAGCCCCA 
GCCAAAGCAG CCCCAGCCAA AGCTGCACCT GCTAAGAAGC AAGATTCAGA CTCTGAATCA 
TCAAGTTCAG AGGATGAAGA AGAGGTGAAG AAACCTGCTG CAAAGGTGAC CCCTGCTAAG 
GCTACACCGG CTAAGGCTAC ACCGGCTAAG GCTACACCGG CTAAGGCTAC ACCGGCTAAA 
GCTACACCTG CCAAGACTCC CCCAGCTAAA AAGGAGGAGT CCTCAAGCTC AGATTCAGAT 
AGCAGCTCTG AAGATGAGGC TCCTTCAAAA CCTGCCGCGA AAACTGCAGC CCCACCGAAA 
CCTGCTGCTG CTTCCAAAGC CCCCACCAAG AAAGCTGAGA GCAGCTCTGA TTCAGAGGAC 
TCATCCGAAG ATGAGGAAGA AGCAGTTAAA GCCAAACCCA AAGCAACTAA ATCAACGACC 
CCAGCCTCAA AGCCTGCTAC GCCTGCAGCT AAGACCCCTG CTGCAGCAGA GAGTAGCTCA 
GACTCTGACT CATCCTCTGA GGAAGAAGAA GAAACGGTCA AGTCTAAGCC TGCAGCAACT 
AAACTAGCTA CCCCTGCTTC AAAGCCTGCC ACTCCTGCTA CAAAGCCTGC TGCTGCAGCA 
GAGAGCAGTT CTGACTCTGA CTCCTCTGAG GAAGAAGAAC CAGCTAAAGC TAAGCCTGCT 
ACCCCTGCTT CAAAGCCTGC TACCCCCTCA ACTAAGCCTG CTACTCCTGC TTCAAAGCCT 
GCTGCTGCTG CAGCAGAGAG CAGTTCTGAC TCCTCTGAGG ATGAGGAAGA ACCAGCTAAA 
GCTAAGCCAG TAGCAGCAAC TAAACCTGCT ACCCCAGCAG CGAAGCCTGC CACTCCTGCA 
GCAAAGCCTG CAGCAGCAGA GAGCAGCTCA GACTCTGACA GCTCAGACTC TGAGAATGAG 
GCCTCAAAAC CTGCAGTCAA GAAGCCAGCT CCAGCTAAAA CAGTGGCTAA GAAACCTGCT 
GCTGCTGCAA AGGCCCCCGC AGACTCCAGT GATGACAGCT CTAGTGATGA GGAGGAACCC 
AAGAAGGCAG CACCACAGAC CCCCAAACCT GCAGCCACAA AGGCAGCAGC TGCTCCACCA 
AAGAAGACTC AGGAGAGCAG CTCTGACTCC TCAGACAGCT CTGACTCTGA GGCAGAGACC 
AAGTCCACCC CTGCTAAGCC TGCGGTCACA AACGGCAAGG CAGCAACACC CAAGACCCCA 
GCCAAGACCC CAGCCAAGAC CCCAGCAAAG CCAGAAGAGT CTTCATCCAG TGACAGCAGC 
TCCGAGGAGG AGGAAGAGGC CAGTAAAAGC ACTAAAGTAA AAAAACCTGC AACACCAGTT 
GCAACAGCAA AGACGACTCC AGCAGCGAAA CCTAAAGAGA GCAGCTCCTC AGACAGTTCA 
TCAGATGAGG ACGAAGCTCC ACAGAAAGCA GCCACAGCAC CCACCACCCC CACAACAAAT 
GGTACCAATG GAAAGAGAAA AAAAGATGAC GAATCATCAG AAAGTGAAGA CGAAGATACA 
GAGGTCACAA CCCCAAAAAA CAAGAAGGCA GCAACCACAC CTCAGACTTT CCCTAAAGCC 
AATAAGAAGT CTTCCAACGT ACCTTTCCGT AGAATAAGGG AGGAACAAGT TGATGTGGAT 
CCCCGTCTTG CAGATAACTC CTTTGATGCA AAGGGTGGAT CCAGAGGTGA CTGGGGCGAG 
AAAGCAAACG ATGTGCTCAG GTTCACGAAA GGCAAATCTT TCCGCCACGA AAAGACCAAG 
AAGAAGAGGG GAAGCTACCG CGGCGGAGCC ATCTCCACCA CTATCAACTC CATTAAGTTT 
GACAGTGATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019950962.1
CDS	32..3103
Translation	MRRSSAHFFPSRKSVVKMAEKSSVPSDLYKCVYSFLRENKFTRAAQQFLKQTQVTPQDENEENLVNIYNFWVKSPESKKRKAPPTKAEAGDGPSSKKAKPSTESSSSEESSSEDEEAAAKPAKAAAAAAKVVPAKAAAAKAAAAKAASSSSEDSSDSEEEKAPVKAPVKKAAAVKKVVAAPAAKKVVAAPAAGGTRKKDSSSSSEESDSEEEEPAKAPAPKPKPGAVTTSKPAAPAKGAGQKKQESTSEDSSSDSEAEEPAKAPAKPTPVKAAAAAESTSEDSSSEDEAPPSKKPKAGAYSAVPPPASVQKAPAAPAASKAKDSDSSDSSDDSSDEEEEKKVAAKPAPVKTPAVKPAAAKPAAKKQESSSESSDSSSEEEAKKTPAKVTPAKAAPAKAAPAKAAPAKAAPAKAAPAKAAPAKAAPAKAAPAKKQDSDSESSSSEDEEEVKKPAAKVTPAKATPAKATPAKATPAKATPAKATPAKTPPAKKEESSSSDSDSSSEDEAPSKPAAKTAAPPKPAAASKAPTKKAESSSDSEDSSEDEEEAVKAKPKATKSTTPASKPATPAAKTPAAAESSSDSDSSSEEEEETVKSKPAATKLATPASKPATPATKPAAAAESSSDSDSSEEEEPAKAKPATPASKPATPSTKPATPASKPAAAAAESSSDSSEDEEEPAKAKPVAATKPATPAAKPATPAAKPAAAESSSDSDSSDSENEASKPAVKKPAPAKTVAKKPAAAAKAPADSSDDSSSDEEEPKKAAPQTPKPAATKAAAAPPKKTQESSSDSSDSSDSEAETKSTPAKPAVTNGKAATPKTPAKTPAKTPAKPEESSSSDSSSEEEEEASKSTKVKKPATPVATAKTTPAAKPKESSSSDSSSDEDEAPQKAATAPTTPTTNGTNGKRKKDDESSESEDEDTEVTTPKNKKAATTPQTFPKANKKSSNVPFRRIREEQVDVDPRLADNSFDAKGGSRGDWGEKANDVLRFTKGKSFRHEKTKKKRGSYRGGAISTTINSIKFDSD

Target ORF information:

RefSeq Version	XM_020095403.1
Organism	Paralichthys olivaceus(Japanese flounder)
Definition	Paralichthys olivaceus nucleolar and coiled-body phosphoprotein 1 (nolc1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020095403.1

ORF Insert Sequence:

ATGCGCCGGA GCTCCGCACA CTTCTTTCCT TCCAGAAAGA GCGTGGTAAA GATGGCGGAG 
AAGAGCTCGG TGCCGAGTGA TCTCTACAAG TGCGTTTATT CATTTCTACG GGAAAACAAG 
TTCACCAGGG CGGCGCAGCA GTTCCTGAAG CAGACCCAAG TGACTCCCCA AGACGAAAAT 
GAGGAAAACC TCGTCAACAT CTACAACTTC TGGGTGAAGT CTCCTGAATC CAAGAAACGA 
AAAGCGCCTC CTACCAAGGC TGAAGCTGGC GACGGTCCAT CGTCCAAGAA AGCAAAGCCA 
AGTACAGAGT CCTCCAGCAG CGAAGAGTCG AGCAGCGAGG ATGAAGAAGC TGCTGCAAAA 
CCAGCTAAGG CAGCCGCTGC AGCAGCTAAA GTGGTCCCTG CTAAAGCAGC AGCTGCTAAA 
GCAGCAGCTG CTAAAGCTGC ATCCAGCAGC AGTGAAGACT CCAGTGACTC TGAGGAGGAG 
AAGGCTCCTG TAAAGGCTCC TGTGAAGAAG GCTGCTGCTG TGAAGAAGGT TGTTGCTGCT 
CCTGCTGCGA AGAAGGTTGT TGCTGCTCCT GCTGCAGGTG GCACAAGGAA GAAAGACAGC 
AGCTCAAGCA GCGAAGAGTC CGACTCTGAG GAGGAGGAGC CGGCTAAAGC CCCTGCACCA 
AAACCCAAGC CGGGTGCCGT CACTACATCT AAACCTGCTG CTCCTGCTAA AGGTGCAGGT 
CAGAAAAAAC AGGAGAGCAC CAGTGAAGAC AGTTCCTCAG ATTCTGAAGC TGAAGAACCA 
GCAAAGGCTC CAGCCAAACC CACCCCAGTA AAAGCTGCTG CTGCAGCCGA ATCAACCAGT 
GAAGACTCTT CATCCGAAGA TGAGGCTCCT CCCAGTAAAA AACCCAAAGC AGGAGCATAC 
AGCGCCGTAC CACCTCCTGC ATCAGTCCAG AAAGCACCAG CCGCACCGGC TGCCAGCAAG 
GCCAAGGACA GCGACTCCTC AGACAGCAGT GATGACAGCA GTGATGAGGA AGAAGAGAAG 
AAAGTGGCTG CAAAACCTGC CCCAGTTAAG ACCCCTGCTG TCAAACCTGC CGCGGCCAAA 
CCTGCTGCAA AGAAGCAGGA ATCCAGCTCA GAGAGCTCAG ATTCAAGCTC AGAAGAAGAG 
GCAAAGAAGA CCCCAGCCAA AGTCACCCCA GCTAAAGCAG CCCCAGCCAA AGCAGCCCCA 
GCTAAAGCAG CCCCAGCTAA AGCAGCCCCA GCTAAAGCAG CCCCAGCCAA AGCAGCCCCA 
GCCAAAGCAG CCCCAGCCAA AGCTGCACCT GCTAAGAAGC AAGATTCAGA CTCTGAATCA 
TCAAGTTCAG AGGATGAAGA AGAGGTGAAG AAACCTGCTG CAAAGGTGAC CCCTGCTAAG 
GCTACACCGG CTAAGGCTAC ACCGGCTAAG GCTACACCGG CTAAGGCTAC ACCGGCTAAA 
GCTACACCTG CCAAGACTCC CCCAGCTAAA AAGGAGGAGT CCTCAAGCTC AGATTCAGAT 
AGCAGCTCTG AAGATGAGGC TCCTTCAAAA CCTGCCGCGA AAACTGCAGC CCCACCGAAA 
CCTGCTGCTG CTTCCAAAGC CCCCACCAAG AAAGCTGAGA GCAGCTCTGA TTCAGAGGAC 
TCATCCGAAG ATGAGGAAGA AGCAGTTAAA GCCAAACCCA AAGCAACTAA ATCAACGACC 
CCAGCCTCAA AGCCTGCTAC GCCTGCAGCT AAGACCCCTG CTGCAGCAGA GAGTAGCTCA 
GACTCTGACT CATCCTCTGA GGAAGAAGAA GAAACGGTCA AGTCTAAGCC TGCAGCAACT 
AAACTAGCTA CCCCTGCTTC AAAGCCTGCC ACTCCTGCTA CAAAGCCTGC TGCTGCAGCA 
GAGAGCAGTT CTGACTCTGA CTCCTCTGAG GAAGAAGAAC CAGCTAAAGC TAAGCCTGCT 
ACCCCTGCTT CAAAGCCTGC TACCCCCTCA ACTAAGCCTG CTACTCCTGC TTCAAAGCCT 
GCTGCTGCTG CAGCAGAGAG CAGTTCTGAC TCCTCTGAGG ATGAGGAAGA ACCAGCTAAA 
GCTAAGCCAG TAGCAGCAAC TAAACCTGCT ACCCCAGCAG CGAAGCCTGC CACTCCTGCA 
GCAAAGCCTG CAGCAGCAGA GAGCAGCTCA GACTCTGACA GCTCAGACTC TGAGAATGAG 
GCCTCAAAAC CTGCAGTCAA GAAGCCAGCT CCAGCTAAAA CAGTGGCTAA GAAACCTGCT 
GCTGCTGCAA AGGCCCCCGC AGACTCCAGT GATGACAGCT CTAGTGATGA GGAGGAACCC 
AAGAAGGCAG CACCACAGAC CCCCAAACCT GCAGCCACAA AGGCAGCAGC TGCTCCACCA 
AAGAAGACTC AGGAGAGCAG CTCTGACTCC TCAGACAGCT CTGACTCTGA GGCAGAGACC 
AAGTCCACCC CTGCTAAGCC TGCGGTCACA AACGGCAAGG CAGCAACACC CAAGACCCCA 
GCCAAGACCC CAGCCAAGAC CCCAGCAAAG CCAGAAGAGT CTTCATCCAG TGACAGCAGC 
TCCGAGGAGG AGGAAGAGGC CAGTAAAAGC ACTAAAGTAA AAAAACCTGC AACACCAGTT 
GCAACAGCAA AGACGACTCC AGCAGCGAAA CCTAAAGAGA GCAGCTCCTC AGACAGTTCA 
TCAGATGAGG ACGAAGCTCC ACAGAAAGCA GCCACAGCAC CCACCACCCC CACAACAAAT 
GGTACCAATG GAAAGAGAAA AAAAGATGAC GAATCATCAG AAAGTGAAGA CGAAGATACA 
GAGGTCACAA CCCCAAAAAA CAAGAAGGCA GCAACCACAC CTCAGACTTT CCCTAAAGCC 
AATAAGAAGT CTTCCAACGT ACCTTTCCGT AGAATAAGGG AGGAACAAGT TGATGTGGAT 
CCCCGTCTTG CAGATAACTC CTTTGATGCA AAGGGTGGAT CCAGAGGTGA CTGGGGCGAG 
AAAGCAAACG ATGTGCTCAG GTTCACGAAA GGCAAATCTT TCCGCCACGA AAAGACCAAG 
AAGAAGAGGG GAAGCTACCG CGGCGGAGCC ATCTCCACCA CTATCAACTC CATTAAGTTT 
GACAGTGATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nolc1 ( XM_020095409.1 ) cDNA ORF clone, Paralichthys olivaceus(Japanese flounder) -> XP_019950968.1 Paralichthys olivaceus nucleolar and coiled-body phosphoprotein 1 (nolc1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPa231945
Clone ID Related Accession (Same CDS sequence)	XM_020095409.1
Accession Version	XM_020095409.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3072bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-07
Organism	Paralichthys olivaceus(Japanese flounder)
Product	nucleolar and coiled-body phosphoprotein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017859642.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Paralichthys olivaceus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCCGGA GCTCCGCACA CTTCTTTCCT TCCAGAAAGA GCGTGGTAAA GATGGCGGAG 
AAGAGCTCGG TGCCGAGTGA TCTCTACAAG TGCGTTTATT CATTTCTACG GGAAAACAAG 
TTCACCAGGG CGGCGCAGCA GTTCCTGAAG CAGACCCAAG TGACTCCCCA AGACGAAAAT 
GAGGAAAACC TCGTCAACAT CTACAACTTC TGGGTGAAGT CTCCTGAATC CAAGAAACGA 
AAAGCGCCTC CTACCAAGGC TGAAGCTGGC GACGGTCCAT CGTCCAAGAA AGCAAAGCCA 
AGTACAGAGT CCTCCAGCAG CGAAGAGTCG AGCAGCGAGG ATGAAGAAGC TGCTGCAAAA 
CCAGCTAAGG CAGCCGCTGC AGCAGCTAAA GTGGTCCCTG CTAAAGCAGC AGCTGCTAAA 
GCAGCAGCTG CTAAAGCTGC ATCCAGCAGC AGTGAAGACT CCAGTGACTC TGAGGAGGAG 
AAGGCTCCTG TAAAGGCTCC TGTGAAGAAG GCTGCTGCTG TGAAGAAGGT TGTTGCTGCT 
CCTGCTGCGA AGAAGGTTGT TGCTGCTCCT GCTGCAGGTG GCACAAGGAA GAAAGACAGC 
AGCTCAAGCA GCGAAGAGTC CGACTCTGAG GAGGAGGAGC CGGCTAAAGC CCCTGCACCA 
AAACCCAAGC CGGGTGCCGT CACTACATCT AAACCTGCTG CTCCTGCTAA AGGTGCAGGT 
CAGAAAAAAC AGGAGAGCAC CAGTGAAGAC AGTTCCTCAG ATTCTGAAGC TGAAGAACCA 
GCAAAGGCTC CAGCCAAACC CACCCCAGTA AAAGCTGCTG CTGCAGCCGA ATCAACCAGT 
GAAGACTCTT CATCCGAAGA TGAGGCTCCT CCCAGTAAAA AACCCAAAGC AGGAGCATAC 
AGCGCCGTAC CACCTCCTGC ATCAGTCCAG AAAGCACCAG CCGCACCGGC TGCCAGCAAG 
GCCAAGGACA GCGACTCCTC AGACAGCAGT GATGACAGCA GTGATGAGGA AGAAGAGAAG 
AAAGTGGCTG CAAAACCTGC CCCAGTTAAG ACCCCTGCTG TCAAACCTGC CGCGGCCAAA 
CCTGCTGCAA AGAAGCAGGA ATCCAGCTCA GAGAGCTCAG ATTCAAGCTC AGAAGAAGAG 
GCAAAGAAGA CCCCAGCCAA AGTCACCCCA GCTAAAGCAG CCCCAGCCAA AGCAGCCCCA 
GCTAAAGCAG CCCCAGCTAA AGCAGCCCCA GCTAAAGCAG CCCCAGCCAA AGCAGCCCCA 
GCCAAAGCAG CCCCAGCCAA AGCTGCACCT GCTAAGAAGC AAGATTCAGA CTCTGAATCA 
TCAAGTTCAG AGGATGAAGA AGAGGTGAAG AAACCTGCTG CAAAGGTGAC CCCTGCTAAG 
GCTACACCGG CTAAGGCTAC ACCGGCTAAG GCTACACCGG CTAAGGCTAC ACCGGCTAAA 
GCTACACCTG CCAAGACTCC CCCAGCTAAA AAGGAGGAGT CCTCAAGCTC AGATTCAGAT 
AGCAGCTCTG AAGATGAGGC TCCTTCAAAA CCTGCCGCGA AAACTGCAGC CCCACCGAAA 
CCTGCTGCTG CTTCCAAAGC CCCCACCAAG AAAGCTGAGA GCAGCTCTGA TTCAGAGGAC 
TCATCCGAAG ATGAGGAAGA AGCAGTTAAA GCCAAACCCA AAGCAACTAA ATCAACGACC 
CCAGCCTCAA AGCCTGCTAC GCCTGCAGCT AAGACCCCTG CTGCAGCAGA GAGTAGCTCA 
GACTCTGACT CATCCTCTGA GGAAGAAGAA GAAACGGTCA AGTCTAAGCC TGCAGCAACT 
AAACTAGCTA CCCCTGCTTC AAAGCCTGCC ACTCCTGCTA CAAAGCCTGC TGCTGCAGCA 
GAGAGCAGTT CTGACTCTGA CTCCTCTGAG GAAGAAGAAC CAGCTAAAGC TAAGCCTGCT 
ACCCCTGCTT CAAAGGCTGC TACCCCCTCA ACTAAGCCTG CTACTCCTGC TTCAAAGCCT 
GCTGCTGCTG CAGCAGAGAG CAGTTCTGAC TCCTCTGAGG ATGAGGAAGA ACCAGCTAAA 
GCTAAGCCAG TAGCAGCAAC TAAACCTGCT ACCCCAGCAG CGAAGCCTGC CACTCCTGCA 
GCAAAGCCTG CAGCAGCAGA GAGCAGCTCA GACTCTGACA GCTCAGACTC TGAGAATGAG 
GCCTCAAAAC CTGCAGTCAA GAAGCCAGCT CCAGCTAAAA CAGTGGCTAA GAAACCTGCT 
GCTGCTGCAA AGGCCCCCGC AGACTCCAGT GATGACAGCT CTAGTGATGA GGAGGAACCC 
AAGAAGGCAG CACCACAGAC CCCCAAACCT GCAGCCACAA AGGCAGCAGC TGCTCCACCA 
AAGAAGACTC AGGAGAGCAG CTCTGACTCC TCAGACAGCT CTGACTCTGA GGCAGAGACC 
AAGTCCACCC CTGCTAAGCC TGCGGTCACA AACGGCAAGG CAGCAACACC CAAGACCCCA 
GCCAAGACCC CAGCCAAGAC CCCAGCAAAG CCAGAAGAGT CTTCATCCAG TGACAGCAGC 
TCCGAGGAGG AGGAAGAGGC CAGTAAAAGC ACTAAAGTAA AAAAACCTGC AACACCAGTT 
GCAACAGCAA AGACGACTCC AGCAGCGAAA CCTAAAGAGA GCAGCTCCTC AGACAGTTCA 
TCAGATGAGG ACGAAGCTCC ACAGAAAGCA GCCACAGCAC CCACCACCCC CACAACAAAT 
GGTACCAATG GAAAGAGAAA AAAAGATGAC GAATCATCAG AAAGTGAAGA CGAAGATACA 
GAGGTCACAA CCCCAAAAAA CAAGAAGGCA GCAACCACAC CTCAGACTTT CCCTAAAGCC 
AATAAGAAGT CTTCCAACGT ACCTTTCCGT AGAATAAGGG AGGAACAAGT TGATGTGGAT 
CCCCGTCTTG CAGATAACTC CTTTGATGCA AAGGGTGGAT CCAGAGGTGA CTGGGGCGAG 
AAAGCAAACG ATGTGCTCAG GTTCACGAAA GGCAAATCTT TCCGCCACGA AAAGACCAAG 
AAGAAGAGGG GAAGCTACCG CGGCGGAGCC ATCTCCACCA CTATCAACTC CATTAAGTTT 
GACAGTGATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019950968.1
CDS	32..3103
Translation	MRRSSAHFFPSRKSVVKMAEKSSVPSDLYKCVYSFLRENKFTRAAQQFLKQTQVTPQDENEENLVNIYNFWVKSPESKKRKAPPTKAEAGDGPSSKKAKPSTESSSSEESSSEDEEAAAKPAKAAAAAAKVVPAKAAAAKAAAAKAASSSSEDSSDSEEEKAPVKAPVKKAAAVKKVVAAPAAKKVVAAPAAGGTRKKDSSSSSEESDSEEEEPAKAPAPKPKPGAVTTSKPAAPAKGAGQKKQESTSEDSSSDSEAEEPAKAPAKPTPVKAAAAAESTSEDSSSEDEAPPSKKPKAGAYSAVPPPASVQKAPAAPAASKAKDSDSSDSSDDSSDEEEEKKVAAKPAPVKTPAVKPAAAKPAAKKQESSSESSDSSSEEEAKKTPAKVTPAKAAPAKAAPAKAAPAKAAPAKAAPAKAAPAKAAPAKAAPAKKQDSDSESSSSEDEEEVKKPAAKVTPAKATPAKATPAKATPAKATPAKATPAKTPPAKKEESSSSDSDSSSEDEAPSKPAAKTAAPPKPAAASKAPTKKAESSSDSEDSSEDEEEAVKAKPKATKSTTPASKPATPAAKTPAAAESSSDSDSSSEEEEETVKSKPAATKLATPASKPATPATKPAAAAESSSDSDSSEEEEPAKAKPATPASKAATPSTKPATPASKPAAAAAESSSDSSEDEEEPAKAKPVAATKPATPAAKPATPAAKPAAAESSSDSDSSDSENEASKPAVKKPAPAKTVAKKPAAAAKAPADSSDDSSSDEEEPKKAAPQTPKPAATKAAAAPPKKTQESSSDSSDSSDSEAETKSTPAKPAVTNGKAATPKTPAKTPAKTPAKPEESSSSDSSSEEEEEASKSTKVKKPATPVATAKTTPAAKPKESSSSDSSSDEDEAPQKAATAPTTPTTNGTNGKRKKDDESSESEDEDTEVTTPKNKKAATTPQTFPKANKKSSNVPFRRIREEQVDVDPRLADNSFDAKGGSRGDWGEKANDVLRFTKGKSFRHEKTKKKRGSYRGGAISTTINSIKFDSD

Target ORF information:

RefSeq Version	XM_020095409.1
Organism	Paralichthys olivaceus(Japanese flounder)
Definition	Paralichthys olivaceus nucleolar and coiled-body phosphoprotein 1 (nolc1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020095409.1

ORF Insert Sequence:

ATGCGCCGGA GCTCCGCACA CTTCTTTCCT TCCAGAAAGA GCGTGGTAAA GATGGCGGAG 
AAGAGCTCGG TGCCGAGTGA TCTCTACAAG TGCGTTTATT CATTTCTACG GGAAAACAAG 
TTCACCAGGG CGGCGCAGCA GTTCCTGAAG CAGACCCAAG TGACTCCCCA AGACGAAAAT 
GAGGAAAACC TCGTCAACAT CTACAACTTC TGGGTGAAGT CTCCTGAATC CAAGAAACGA 
AAAGCGCCTC CTACCAAGGC TGAAGCTGGC GACGGTCCAT CGTCCAAGAA AGCAAAGCCA 
AGTACAGAGT CCTCCAGCAG CGAAGAGTCG AGCAGCGAGG ATGAAGAAGC TGCTGCAAAA 
CCAGCTAAGG CAGCCGCTGC AGCAGCTAAA GTGGTCCCTG CTAAAGCAGC AGCTGCTAAA 
GCAGCAGCTG CTAAAGCTGC ATCCAGCAGC AGTGAAGACT CCAGTGACTC TGAGGAGGAG 
AAGGCTCCTG TAAAGGCTCC TGTGAAGAAG GCTGCTGCTG TGAAGAAGGT TGTTGCTGCT 
CCTGCTGCGA AGAAGGTTGT TGCTGCTCCT GCTGCAGGTG GCACAAGGAA GAAAGACAGC 
AGCTCAAGCA GCGAAGAGTC CGACTCTGAG GAGGAGGAGC CGGCTAAAGC CCCTGCACCA 
AAACCCAAGC CGGGTGCCGT CACTACATCT AAACCTGCTG CTCCTGCTAA AGGTGCAGGT 
CAGAAAAAAC AGGAGAGCAC CAGTGAAGAC AGTTCCTCAG ATTCTGAAGC TGAAGAACCA 
GCAAAGGCTC CAGCCAAACC CACCCCAGTA AAAGCTGCTG CTGCAGCCGA ATCAACCAGT 
GAAGACTCTT CATCCGAAGA TGAGGCTCCT CCCAGTAAAA AACCCAAAGC AGGAGCATAC 
AGCGCCGTAC CACCTCCTGC ATCAGTCCAG AAAGCACCAG CCGCACCGGC TGCCAGCAAG 
GCCAAGGACA GCGACTCCTC AGACAGCAGT GATGACAGCA GTGATGAGGA AGAAGAGAAG 
AAAGTGGCTG CAAAACCTGC CCCAGTTAAG ACCCCTGCTG TCAAACCTGC CGCGGCCAAA 
CCTGCTGCAA AGAAGCAGGA ATCCAGCTCA GAGAGCTCAG ATTCAAGCTC AGAAGAAGAG 
GCAAAGAAGA CCCCAGCCAA AGTCACCCCA GCTAAAGCAG CCCCAGCCAA AGCAGCCCCA 
GCTAAAGCAG CCCCAGCTAA AGCAGCCCCA GCTAAAGCAG CCCCAGCCAA AGCAGCCCCA 
GCCAAAGCAG CCCCAGCCAA AGCTGCACCT GCTAAGAAGC AAGATTCAGA CTCTGAATCA 
TCAAGTTCAG AGGATGAAGA AGAGGTGAAG AAACCTGCTG CAAAGGTGAC CCCTGCTAAG 
GCTACACCGG CTAAGGCTAC ACCGGCTAAG GCTACACCGG CTAAGGCTAC ACCGGCTAAA 
GCTACACCTG CCAAGACTCC CCCAGCTAAA AAGGAGGAGT CCTCAAGCTC AGATTCAGAT 
AGCAGCTCTG AAGATGAGGC TCCTTCAAAA CCTGCCGCGA AAACTGCAGC CCCACCGAAA 
CCTGCTGCTG CTTCCAAAGC CCCCACCAAG AAAGCTGAGA GCAGCTCTGA TTCAGAGGAC 
TCATCCGAAG ATGAGGAAGA AGCAGTTAAA GCCAAACCCA AAGCAACTAA ATCAACGACC 
CCAGCCTCAA AGCCTGCTAC GCCTGCAGCT AAGACCCCTG CTGCAGCAGA GAGTAGCTCA 
GACTCTGACT CATCCTCTGA GGAAGAAGAA GAAACGGTCA AGTCTAAGCC TGCAGCAACT 
AAACTAGCTA CCCCTGCTTC AAAGCCTGCC ACTCCTGCTA CAAAGCCTGC TGCTGCAGCA 
GAGAGCAGTT CTGACTCTGA CTCCTCTGAG GAAGAAGAAC CAGCTAAAGC TAAGCCTGCT 
ACCCCTGCTT CAAAGGCTGC TACCCCCTCA ACTAAGCCTG CTACTCCTGC TTCAAAGCCT 
GCTGCTGCTG CAGCAGAGAG CAGTTCTGAC TCCTCTGAGG ATGAGGAAGA ACCAGCTAAA 
GCTAAGCCAG TAGCAGCAAC TAAACCTGCT ACCCCAGCAG CGAAGCCTGC CACTCCTGCA 
GCAAAGCCTG CAGCAGCAGA GAGCAGCTCA GACTCTGACA GCTCAGACTC TGAGAATGAG 
GCCTCAAAAC CTGCAGTCAA GAAGCCAGCT CCAGCTAAAA CAGTGGCTAA GAAACCTGCT 
GCTGCTGCAA AGGCCCCCGC AGACTCCAGT GATGACAGCT CTAGTGATGA GGAGGAACCC 
AAGAAGGCAG CACCACAGAC CCCCAAACCT GCAGCCACAA AGGCAGCAGC TGCTCCACCA 
AAGAAGACTC AGGAGAGCAG CTCTGACTCC TCAGACAGCT CTGACTCTGA GGCAGAGACC 
AAGTCCACCC CTGCTAAGCC TGCGGTCACA AACGGCAAGG CAGCAACACC CAAGACCCCA 
GCCAAGACCC CAGCCAAGAC CCCAGCAAAG CCAGAAGAGT CTTCATCCAG TGACAGCAGC 
TCCGAGGAGG AGGAAGAGGC CAGTAAAAGC ACTAAAGTAA AAAAACCTGC AACACCAGTT 
GCAACAGCAA AGACGACTCC AGCAGCGAAA CCTAAAGAGA GCAGCTCCTC AGACAGTTCA 
TCAGATGAGG ACGAAGCTCC ACAGAAAGCA GCCACAGCAC CCACCACCCC CACAACAAAT 
GGTACCAATG GAAAGAGAAA AAAAGATGAC GAATCATCAG AAAGTGAAGA CGAAGATACA 
GAGGTCACAA CCCCAAAAAA CAAGAAGGCA GCAACCACAC CTCAGACTTT CCCTAAAGCC 
AATAAGAAGT CTTCCAACGT ACCTTTCCGT AGAATAAGGG AGGAACAAGT TGATGTGGAT 
CCCCGTCTTG CAGATAACTC CTTTGATGCA AAGGGTGGAT CCAGAGGTGA CTGGGGCGAG 
AAAGCAAACG ATGTGCTCAG GTTCACGAAA GGCAAATCTT TCCGCCACGA AAAGACCAAG 
AAGAAGAGGG GAAGCTACCG CGGCGGAGCC ATCTCCACCA CTATCAACTC CATTAAGTTT 
GACAGTGATT GA