tbc1d10b cDNA ORF clone, Paralichthys olivaceus(Japanese flounder)

Gene
Clones
gRNAs

Summary

Gene Symbol	tbc1d10b
Entrez Gene ID	109634264
Full Name	TBC1 domain family member 10B
Gene Type	protein-coding
Organism	Paralichthys olivaceus(Japanese flounder)

mRNA and Protein(s)

mRNA	Protein	Name
XM_020094639.1	XP_019950198.1	TBC1 domain family member 10B
XM_020094640.1	XP_019950199.1	TBC1 domain family member 10B

Pathways from BioSystems
Gene Ontology

The following tbc1d10b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tbc1d10b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPa246370	XM_020094639.1 Latest version!	Paralichthys olivaceus TBC1 domain family member 10B (tbc1d10b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OPa246370	XM_020094640.1 Latest version!	Paralichthys olivaceus TBC1 domain family member 10B (tbc1d10b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tbc1d10b ( XM_020094639.1 ) cDNA ORF clone, Paralichthys olivaceus(Japanese flounder) -> XP_019950198.1 Paralichthys olivaceus TBC1 domain family member 10B (tbc1d10b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPa246370
Clone ID Related Accession (Same CDS sequence)	XM_020094639.1 , XM_020094640.1
Accession Version	XM_020094639.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2772bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-07
Organism	Paralichthys olivaceus(Japanese flounder)
Product	TBC1 domain family member 10B
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017859663.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Paralichthys olivaceus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGAAC TCTCCACCTT ATCCCCATCA GCGCTGAGCG AACCGCATGC AGCTCCTTCC 
ATCCCTCCAT CCTCTGCATC CTCTATCCCT GTACCCAACA ACTCTGCTCC GGAGGCTAAA 
CCAAAGTCTT CCCTCTACAA TGACAAAGCT CTGACTGGAA ACACTTCTAC ATCACTGTCG 
ATGAGTTCAC CTGAGACTCC CCACCCACCC CTGAATCCTC TGAAAACCAC TGACGAGATC 
CCCCTCCCAT CATTGGTACC TGAGGTTCTT CGGAAAGAGG AATCAACAGG GAGAATTGTA 
GAAACTGGAG TTCATAACAT TAAGCTGGAG TCAATGTCCA GTGACCAAGT TGCTGCAGAG 
ATCATGAATA CTGTAACAGA GGCACCAACA CTATTGGGTC AGCCATCAGC TCAGATTGAA 
GAGCAACAGC CATCCCCCTC TCCTGATTTT AATCCCAGCC CTAACCTTTA TCCAAATGTG 
CATGTCACCC ATGCCCCACA TCCTGTCTTG GATGTTGGTC ACTTTTCAGC AGACCAACCC 
CAAACCACTG CAGATTCCAA TCCCACACCT GCTCTTCCAG CATCCACCAA TACAGCATCA 
GCAACAGCCG AAGAGGAGAA TCCTCAGCCA ATTCAACAAA TACAAAACCC CCAAAGTCAG 
CCTCCCCCTC TGTCCACAAA GCCTGACGAC CCGAAGGAAG CGGGGGAAGC AGAGCAGCCT 
AGCACCCCAA CCAGAGGCGA ACACCCTAGC CCCACTGTCC CATTGATCCA TGAACCAGCC 
TGTGCCCTCC CACTGTACAA CCCCAACCCC CGCCCAGCCC CTGATACCCT CAGCTACCTG 
GAATCAGCTA GCCTTATGTC AGGGACGTTG GAGTCCCTGT CTGGATTGGG AGAGGATGGG 
AGCTCTGTTG GCTCAGACTC GGAGATCAAC GGCCTCACTG TCAGGCGGAC AGATAAATAT 
GGCTTCCTGG GTGGTAATCA GTACAGTGAA GGCAGTGAAA AGGATGTCCG AGTTGAAGTT 
GCCAGACAGA GGGAGATGAA ATGGATTGAT ATGTTCAACA ACTGGGATAA GTGGATAAAA 
CATCGCTTCC AGAAGGTGAA GTTGCGCTGC AGGAAGGGCA TTCCATCCTC TCTCAGAGCC 
AAAGCTTGGC AGCTGCTGTC CAATAGCCAG GAGCTCTTAG AAGCCAACAG AGGGAAATTT 
GAGGAGCTGG AAAGAGAGCA GGGTGAGGCT AAATGGTTGG ACATTATAGA GAAGGATCTC 
CACAGACAGT TCCCCTTCCA TGAAATGTTT GCTGCTCGGG GTGGCCATGG CCAACAGGAC 
CTGTACCGCA TCCTGAAAGC CTACACCATC TATCGGCCTG ATGAGGGATA TTGCCAGGCC 
CAAGCTCCTG TCGCTGCAGT CCTGCTCATG CACATGCCTG CTGAGCAAGC GTTTTGGTGC 
CTCGTACAGA TATGTGAGAA ATATCTCCCT GGCTACTACA GCGCGGGGCT GGAAGCGATC 
CAGCTGGATG GCGAGATCTT CTTCTCTTTA CTACGACGCA CATGTCCAAT GGCGTACCGT 
CATCTCAAGA AGTTCAAGAT CGACCCGATT CTCTACATGA CCGAGTGGTT CATGTGCATC 
TTCTCACGCA CTTTACCATG GGCCTGTGTA CTGCGTGTAT GGGACATGTT CTTCTGTGAA 
GGAGTGAAAA TAGTGTTTCG GGTGGGCTTG GTGCTTCTGA AACAAATGCT TGGCTCAGTG 
GATAAACTTC GAGAACTGCA GGGCATGTAT GAAACAATGG AGCGCCTGAG AAACATTTCT 
CCTGAAAGTG TCAGAGAGGA CATAATAGTA CAGGAGATTG TTATACTCCC AGTGACGGAG 
TCGCTCATTG AGAGGGAGTG CAGCATCCAG GTGAGGAAGT GGAGGGAGTC CAGGGGGGAG 
CTGACACACC AGCCGGGCCG CCGGCTCCAT GGTACGAGAG CCATCTTTGA GTGCAAACGA 
CGCGCTGCCT CCATCAGCTC AGGTGGCAGC TTCTCTTTCC TTGGAAGCTC GATCCCTCCA 
CCAGGACCAC TCAGGGCCTC ATCCAGCCTC CTCTCGCTCC CGGGCTTCCG CAAGTCTAGG 
GCTCCTTTCC ACTCTCAAAC CAAGAAGGGT TCCTTCTCAG GGCCCTCAGG AATGGAGAGC 
CTGCTGCCTC AGTCGCCACC TGTAGTAACA TCAAGCCCTG GTCATGGCCC CAACCACAAA 
CCCCCCATTC CCCCAGGAGC AGGTCAGAGG GCACCACCAG CCCACGTTCA GAGCCCCTTG 
GTTGTGAGTG CGGCTGGCCC ATCACGTGAG CTTGCTCAGC CCTCAGAAGG CTCCTTAGCA 
CAAGGCCAGC CTGCAGCTTC AGCCCCAGTA CAGAGCACAC CACCACCTCC ACCCACCACT 
CTATCCCCCT CCCCGAAGAT CGTCTCGGAG CAAATTACTC CCACTATCCC TTCACCTACT 
GCAAACAACT CCCCTCTGCC TCCATGTCCA GAGACAAAAA AAGAAGCGGT GCCAGTGGCT 
GACGCCACAA CTGGAGAGGA GGAGGGAAAA AAGAAGAAGA AAAGCAAAGA AAACAACAAG 
AAAAAGGAAA AGGAAGATGA AAAGAAAAAA ATGAAGGAAA AGAAGGAAAA GGATAAGGCT 
GAAAAAGAGA GGCTCAGAAG GGAGAAGGAG AGGCTGGAAA AAGAGAAGAA GAAAGGGGGG 
AAGAAAAAGG ACAAAGGGGG AGGAGAGGCT GAAGACAAAA ATGGAGCTGC AGCAGCGAAA 
GATTCGGCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019950198.1
CDS	553..3324
Translation	MAELSTLSPSALSEPHAAPSIPPSSASSIPVPNNSAPEAKPKSSLYNDKALTGNTSTSLSMSSPETPHPPLNPLKTTDEIPLPSLVPEVLRKEESTGRIVETGVHNIKLESMSSDQVAAEIMNTVTEAPTLLGQPSAQIEEQQPSPSPDFNPSPNLYPNVHVTHAPHPVLDVGHFSADQPQTTADSNPTPALPASTNTASATAEEENPQPIQQIQNPQSQPPPLSTKPDDPKEAGEAEQPSTPTRGEHPSPTVPLIHEPACALPLYNPNPRPAPDTLSYLESASLMSGTLESLSGLGEDGSSVGSDSEINGLTVRRTDKYGFLGGNQYSEGSEKDVRVEVARQREMKWIDMFNNWDKWIKHRFQKVKLRCRKGIPSSLRAKAWQLLSNSQELLEANRGKFEELEREQGEAKWLDIIEKDLHRQFPFHEMFAARGGHGQQDLYRILKAYTIYRPDEGYCQAQAPVAAVLLMHMPAEQAFWCLVQICEKYLPGYYSAGLEAIQLDGEIFFSLLRRTCPMAYRHLKKFKIDPILYMTEWFMCIFSRTLPWACVLRVWDMFFCEGVKIVFRVGLVLLKQMLGSVDKLRELQGMYETMERLRNISPESVREDIIVQEIVILPVTESLIERECSIQVRKWRESRGELTHQPGRRLHGTRAIFECKRRAASISSGGSFSFLGSSIPPPGPLRASSSLLSLPGFRKSRAPFHSQTKKGSFSGPSGMESLLPQSPPVVTSSPGHGPNHKPPIPPGAGQRAPPAHVQSPLVVSAAGPSRELAQPSEGSLAQGQPAASAPVQSTPPPPPTTLSPSPKIVSEQITPTIPSPTANNSPLPPCPETKKEAVPVADATTGEEEGKKKKKSKENNKKKEKEDEKKKMKEKKEKDKAEKERLRREKERLEKEKKKGGKKKDKGGGEAEDKNGAAAAKDSA

Target ORF information:

RefSeq Version	XM_020094639.1
Organism	Paralichthys olivaceus(Japanese flounder)
Definition	Paralichthys olivaceus TBC1 domain family member 10B (tbc1d10b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020094639.1

ORF Insert Sequence:

ATGGCTGAAC TCTCCACCTT ATCCCCATCA GCGCTGAGCG AACCGCATGC AGCTCCTTCC 
ATCCCTCCAT CCTCTGCATC CTCTATCCCT GTACCCAACA ACTCTGCTCC GGAGGCTAAA 
CCAAAGTCTT CCCTCTACAA TGACAAAGCT CTGACTGGAA ACACTTCTAC ATCACTGTCG 
ATGAGTTCAC CTGAGACTCC CCACCCACCC CTGAATCCTC TGAAAACCAC TGACGAGATC 
CCCCTCCCAT CATTGGTACC TGAGGTTCTT CGGAAAGAGG AATCAACAGG GAGAATTGTA 
GAAACTGGAG TTCATAACAT TAAGCTGGAG TCAATGTCCA GTGACCAAGT TGCTGCAGAG 
ATCATGAATA CTGTAACAGA GGCACCAACA CTATTGGGTC AGCCATCAGC TCAGATTGAA 
GAGCAACAGC CATCCCCCTC TCCTGATTTT AATCCCAGCC CTAACCTTTA TCCAAATGTG 
CATGTCACCC ATGCCCCACA TCCTGTCTTG GATGTTGGTC ACTTTTCAGC AGACCAACCC 
CAAACCACTG CAGATTCCAA TCCCACACCT GCTCTTCCAG CATCCACCAA TACAGCATCA 
GCAACAGCCG AAGAGGAGAA TCCTCAGCCA ATTCAACAAA TACAAAACCC CCAAAGTCAG 
CCTCCCCCTC TGTCCACAAA GCCTGACGAC CCGAAGGAAG CGGGGGAAGC AGAGCAGCCT 
AGCACCCCAA CCAGAGGCGA ACACCCTAGC CCCACTGTCC CATTGATCCA TGAACCAGCC 
TGTGCCCTCC CACTGTACAA CCCCAACCCC CGCCCAGCCC CTGATACCCT CAGCTACCTG 
GAATCAGCTA GCCTTATGTC AGGGACGTTG GAGTCCCTGT CTGGATTGGG AGAGGATGGG 
AGCTCTGTTG GCTCAGACTC GGAGATCAAC GGCCTCACTG TCAGGCGGAC AGATAAATAT 
GGCTTCCTGG GTGGTAATCA GTACAGTGAA GGCAGTGAAA AGGATGTCCG AGTTGAAGTT 
GCCAGACAGA GGGAGATGAA ATGGATTGAT ATGTTCAACA ACTGGGATAA GTGGATAAAA 
CATCGCTTCC AGAAGGTGAA GTTGCGCTGC AGGAAGGGCA TTCCATCCTC TCTCAGAGCC 
AAAGCTTGGC AGCTGCTGTC CAATAGCCAG GAGCTCTTAG AAGCCAACAG AGGGAAATTT 
GAGGAGCTGG AAAGAGAGCA GGGTGAGGCT AAATGGTTGG ACATTATAGA GAAGGATCTC 
CACAGACAGT TCCCCTTCCA TGAAATGTTT GCTGCTCGGG GTGGCCATGG CCAACAGGAC 
CTGTACCGCA TCCTGAAAGC CTACACCATC TATCGGCCTG ATGAGGGATA TTGCCAGGCC 
CAAGCTCCTG TCGCTGCAGT CCTGCTCATG CACATGCCTG CTGAGCAAGC GTTTTGGTGC 
CTCGTACAGA TATGTGAGAA ATATCTCCCT GGCTACTACA GCGCGGGGCT GGAAGCGATC 
CAGCTGGATG GCGAGATCTT CTTCTCTTTA CTACGACGCA CATGTCCAAT GGCGTACCGT 
CATCTCAAGA AGTTCAAGAT CGACCCGATT CTCTACATGA CCGAGTGGTT CATGTGCATC 
TTCTCACGCA CTTTACCATG GGCCTGTGTA CTGCGTGTAT GGGACATGTT CTTCTGTGAA 
GGAGTGAAAA TAGTGTTTCG GGTGGGCTTG GTGCTTCTGA AACAAATGCT TGGCTCAGTG 
GATAAACTTC GAGAACTGCA GGGCATGTAT GAAACAATGG AGCGCCTGAG AAACATTTCT 
CCTGAAAGTG TCAGAGAGGA CATAATAGTA CAGGAGATTG TTATACTCCC AGTGACGGAG 
TCGCTCATTG AGAGGGAGTG CAGCATCCAG GTGAGGAAGT GGAGGGAGTC CAGGGGGGAG 
CTGACACACC AGCCGGGCCG CCGGCTCCAT GGTACGAGAG CCATCTTTGA GTGCAAACGA 
CGCGCTGCCT CCATCAGCTC AGGTGGCAGC TTCTCTTTCC TTGGAAGCTC GATCCCTCCA 
CCAGGACCAC TCAGGGCCTC ATCCAGCCTC CTCTCGCTCC CGGGCTTCCG CAAGTCTAGG 
GCTCCTTTCC ACTCTCAAAC CAAGAAGGGT TCCTTCTCAG GGCCCTCAGG AATGGAGAGC 
CTGCTGCCTC AGTCGCCACC TGTAGTAACA TCAAGCCCTG GTCATGGCCC CAACCACAAA 
CCCCCCATTC CCCCAGGAGC AGGTCAGAGG GCACCACCAG CCCACGTTCA GAGCCCCTTG 
GTTGTGAGTG CGGCTGGCCC ATCACGTGAG CTTGCTCAGC CCTCAGAAGG CTCCTTAGCA 
CAAGGCCAGC CTGCAGCTTC AGCCCCAGTA CAGAGCACAC CACCACCTCC ACCCACCACT 
CTATCCCCCT CCCCGAAGAT CGTCTCGGAG CAAATTACTC CCACTATCCC TTCACCTACT 
GCAAACAACT CCCCTCTGCC TCCATGTCCA GAGACAAAAA AAGAAGCGGT GCCAGTGGCT 
GACGCCACAA CTGGAGAGGA GGAGGGAAAA AAGAAGAAGA AAAGCAAAGA AAACAACAAG 
AAAAAGGAAA AGGAAGATGA AAAGAAAAAA ATGAAGGAAA AGAAGGAAAA GGATAAGGCT 
GAAAAAGAGA GGCTCAGAAG GGAGAAGGAG AGGCTGGAAA AAGAGAAGAA GAAAGGGGGG 
AAGAAAAAGG ACAAAGGGGG AGGAGAGGCT GAAGACAAAA ATGGAGCTGC AGCAGCGAAA 
GATTCGGCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tbc1d10b ( XM_020094640.1 ) cDNA ORF clone, Paralichthys olivaceus(Japanese flounder) -> XP_019950199.1 Paralichthys olivaceus TBC1 domain family member 10B (tbc1d10b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPa246370
Clone ID Related Accession (Same CDS sequence)	XM_020094639.1 , XM_020094640.1
Accession Version	XM_020094640.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2772bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-07
Organism	Paralichthys olivaceus(Japanese flounder)
Product	TBC1 domain family member 10B
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017859663.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Paralichthys olivaceus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGAAC TCTCCACCTT ATCCCCATCA GCGCTGAGCG AACCGCATGC AGCTCCTTCC 
ATCCCTCCAT CCTCTGCATC CTCTATCCCT GTACCCAACA ACTCTGCTCC GGAGGCTAAA 
CCAAAGTCTT CCCTCTACAA TGACAAAGCT CTGACTGGAA ACACTTCTAC ATCACTGTCG 
ATGAGTTCAC CTGAGACTCC CCACCCACCC CTGAATCCTC TGAAAACCAC TGACGAGATC 
CCCCTCCCAT CATTGGTACC TGAGGTTCTT CGGAAAGAGG AATCAACAGG GAGAATTGTA 
GAAACTGGAG TTCATAACAT TAAGCTGGAG TCAATGTCCA GTGACCAAGT TGCTGCAGAG 
ATCATGAATA CTGTAACAGA GGCACCAACA CTATTGGGTC AGCCATCAGC TCAGATTGAA 
GAGCAACAGC CATCCCCCTC TCCTGATTTT AATCCCAGCC CTAACCTTTA TCCAAATGTG 
CATGTCACCC ATGCCCCACA TCCTGTCTTG GATGTTGGTC ACTTTTCAGC AGACCAACCC 
CAAACCACTG CAGATTCCAA TCCCACACCT GCTCTTCCAG CATCCACCAA TACAGCATCA 
GCAACAGCCG AAGAGGAGAA TCCTCAGCCA ATTCAACAAA TACAAAACCC CCAAAGTCAG 
CCTCCCCCTC TGTCCACAAA GCCTGACGAC CCGAAGGAAG CGGGGGAAGC AGAGCAGCCT 
AGCACCCCAA CCAGAGGCGA ACACCCTAGC CCCACTGTCC CATTGATCCA TGAACCAGCC 
TGTGCCCTCC CACTGTACAA CCCCAACCCC CGCCCAGCCC CTGATACCCT CAGCTACCTG 
GAATCAGCTA GCCTTATGTC AGGGACGTTG GAGTCCCTGT CTGGATTGGG AGAGGATGGG 
AGCTCTGTTG GCTCAGACTC GGAGATCAAC GGCCTCACTG TCAGGCGGAC AGATAAATAT 
GGCTTCCTGG GTGGTAATCA GTACAGTGAA GGCAGTGAAA AGGATGTCCG AGTTGAAGTT 
GCCAGACAGA GGGAGATGAA ATGGATTGAT ATGTTCAACA ACTGGGATAA GTGGATAAAA 
CATCGCTTCC AGAAGGTGAA GTTGCGCTGC AGGAAGGGCA TTCCATCCTC TCTCAGAGCC 
AAAGCTTGGC AGCTGCTGTC CAATAGCCAG GAGCTCTTAG AAGCCAACAG AGGGAAATTT 
GAGGAGCTGG AAAGAGAGCA GGGTGAGGCT AAATGGTTGG ACATTATAGA GAAGGATCTC 
CACAGACAGT TCCCCTTCCA TGAAATGTTT GCTGCTCGGG GTGGCCATGG CCAACAGGAC 
CTGTACCGCA TCCTGAAAGC CTACACCATC TATCGGCCTG ATGAGGGATA TTGCCAGGCC 
CAAGCTCCTG TCGCTGCAGT CCTGCTCATG CACATGCCTG CTGAGCAAGC GTTTTGGTGC 
CTCGTACAGA TATGTGAGAA ATATCTCCCT GGCTACTACA GCGCGGGGCT GGAAGCGATC 
CAGCTGGATG GCGAGATCTT CTTCTCTTTA CTACGACGCA CATGTCCAAT GGCGTACCGT 
CATCTCAAGA AGTTCAAGAT CGACCCGATT CTCTACATGA CCGAGTGGTT CATGTGCATC 
TTCTCACGCA CTTTACCATG GGCCTGTGTA CTGCGTGTAT GGGACATGTT CTTCTGTGAA 
GGAGTGAAAA TAGTGTTTCG GGTGGGCTTG GTGCTTCTGA AACAAATGCT TGGCTCAGTG 
GATAAACTTC GAGAACTGCA GGGCATGTAT GAAACAATGG AGCGCCTGAG AAACATTTCT 
CCTGAAAGTG TCAGAGAGGA CATAATAGTA CAGGAGATTG TTATACTCCC AGTGACGGAG 
TCGCTCATTG AGAGGGAGTG CAGCATCCAG GTGAGGAAGT GGAGGGAGTC CAGGGGGGAG 
CTGACACACC AGCCGGGCCG CCGGCTCCAT GGTACGAGAG CCATCTTTGA GTGCAAACGA 
CGCGCTGCCT CCATCAGCTC AGGTGGCAGC TTCTCTTTCC TTGGAAGCTC GATCCCTCCA 
CCAGGACCAC TCAGGGCCTC ATCCAGCCTC CTCTCGCTCC CGGGCTTCCG CAAGTCTAGG 
GCTCCTTTCC ACTCTCAAAC CAAGAAGGGT TCCTTCTCAG GGCCCTCAGG AATGGAGAGC 
CTGCTGCCTC AGTCGCCACC TGTAGTAACA TCAAGCCCTG GTCATGGCCC CAACCACAAA 
CCCCCCATTC CCCCAGGAGC AGGTCAGAGG GCACCACCAG CCCACGTTCA GAGCCCCTTG 
GTTGTGAGTG CGGCTGGCCC ATCACGTGAG CTTGCTCAGC CCTCAGAAGG CTCCTTAGCA 
CAAGGCCAGC CTGCAGCTTC AGCCCCAGTA CAGAGCACAC CACCACCTCC ACCCACCACT 
CTATCCCCCT CCCCGAAGAT CGTCTCGGAG CAAATTACTC CCACTATCCC TTCACCTACT 
GCAAACAACT CCCCTCTGCC TCCATGTCCA GAGACAAAAA AAGAAGCGGT GCCAGTGGCT 
GACGCCACAA CTGGAGAGGA GGAGGGAAAA AAGAAGAAGA AAAGCAAAGA AAACAACAAG 
AAAAAGGAAA AGGAAGATGA AAAGAAAAAA ATGAAGGAAA AGAAGGAAAA GGATAAGGCT 
GAAAAAGAGA GGCTCAGAAG GGAGAAGGAG AGGCTGGAAA AAGAGAAGAA GAAAGGGGGG 
AAGAAAAAGG ACAAAGGGGG AGGAGAGGCT GAAGACAAAA ATGGAGCTGC AGCAGCGAAA 
GATTCGGCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019950199.1
CDS	550..3321
Translation	MAELSTLSPSALSEPHAAPSIPPSSASSIPVPNNSAPEAKPKSSLYNDKALTGNTSTSLSMSSPETPHPPLNPLKTTDEIPLPSLVPEVLRKEESTGRIVETGVHNIKLESMSSDQVAAEIMNTVTEAPTLLGQPSAQIEEQQPSPSPDFNPSPNLYPNVHVTHAPHPVLDVGHFSADQPQTTADSNPTPALPASTNTASATAEEENPQPIQQIQNPQSQPPPLSTKPDDPKEAGEAEQPSTPTRGEHPSPTVPLIHEPACALPLYNPNPRPAPDTLSYLESASLMSGTLESLSGLGEDGSSVGSDSEINGLTVRRTDKYGFLGGNQYSEGSEKDVRVEVARQREMKWIDMFNNWDKWIKHRFQKVKLRCRKGIPSSLRAKAWQLLSNSQELLEANRGKFEELEREQGEAKWLDIIEKDLHRQFPFHEMFAARGGHGQQDLYRILKAYTIYRPDEGYCQAQAPVAAVLLMHMPAEQAFWCLVQICEKYLPGYYSAGLEAIQLDGEIFFSLLRRTCPMAYRHLKKFKIDPILYMTEWFMCIFSRTLPWACVLRVWDMFFCEGVKIVFRVGLVLLKQMLGSVDKLRELQGMYETMERLRNISPESVREDIIVQEIVILPVTESLIERECSIQVRKWRESRGELTHQPGRRLHGTRAIFECKRRAASISSGGSFSFLGSSIPPPGPLRASSSLLSLPGFRKSRAPFHSQTKKGSFSGPSGMESLLPQSPPVVTSSPGHGPNHKPPIPPGAGQRAPPAHVQSPLVVSAAGPSRELAQPSEGSLAQGQPAASAPVQSTPPPPPTTLSPSPKIVSEQITPTIPSPTANNSPLPPCPETKKEAVPVADATTGEEEGKKKKKSKENNKKKEKEDEKKKMKEKKEKDKAEKERLRREKERLEKEKKKGGKKKDKGGGEAEDKNGAAAAKDSA

Target ORF information:

RefSeq Version	XM_020094640.1
Organism	Paralichthys olivaceus(Japanese flounder)
Definition	Paralichthys olivaceus TBC1 domain family member 10B (tbc1d10b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020094640.1

ORF Insert Sequence:

ATGGCTGAAC TCTCCACCTT ATCCCCATCA GCGCTGAGCG AACCGCATGC AGCTCCTTCC 
ATCCCTCCAT CCTCTGCATC CTCTATCCCT GTACCCAACA ACTCTGCTCC GGAGGCTAAA 
CCAAAGTCTT CCCTCTACAA TGACAAAGCT CTGACTGGAA ACACTTCTAC ATCACTGTCG 
ATGAGTTCAC CTGAGACTCC CCACCCACCC CTGAATCCTC TGAAAACCAC TGACGAGATC 
CCCCTCCCAT CATTGGTACC TGAGGTTCTT CGGAAAGAGG AATCAACAGG GAGAATTGTA 
GAAACTGGAG TTCATAACAT TAAGCTGGAG TCAATGTCCA GTGACCAAGT TGCTGCAGAG 
ATCATGAATA CTGTAACAGA GGCACCAACA CTATTGGGTC AGCCATCAGC TCAGATTGAA 
GAGCAACAGC CATCCCCCTC TCCTGATTTT AATCCCAGCC CTAACCTTTA TCCAAATGTG 
CATGTCACCC ATGCCCCACA TCCTGTCTTG GATGTTGGTC ACTTTTCAGC AGACCAACCC 
CAAACCACTG CAGATTCCAA TCCCACACCT GCTCTTCCAG CATCCACCAA TACAGCATCA 
GCAACAGCCG AAGAGGAGAA TCCTCAGCCA ATTCAACAAA TACAAAACCC CCAAAGTCAG 
CCTCCCCCTC TGTCCACAAA GCCTGACGAC CCGAAGGAAG CGGGGGAAGC AGAGCAGCCT 
AGCACCCCAA CCAGAGGCGA ACACCCTAGC CCCACTGTCC CATTGATCCA TGAACCAGCC 
TGTGCCCTCC CACTGTACAA CCCCAACCCC CGCCCAGCCC CTGATACCCT CAGCTACCTG 
GAATCAGCTA GCCTTATGTC AGGGACGTTG GAGTCCCTGT CTGGATTGGG AGAGGATGGG 
AGCTCTGTTG GCTCAGACTC GGAGATCAAC GGCCTCACTG TCAGGCGGAC AGATAAATAT 
GGCTTCCTGG GTGGTAATCA GTACAGTGAA GGCAGTGAAA AGGATGTCCG AGTTGAAGTT 
GCCAGACAGA GGGAGATGAA ATGGATTGAT ATGTTCAACA ACTGGGATAA GTGGATAAAA 
CATCGCTTCC AGAAGGTGAA GTTGCGCTGC AGGAAGGGCA TTCCATCCTC TCTCAGAGCC 
AAAGCTTGGC AGCTGCTGTC CAATAGCCAG GAGCTCTTAG AAGCCAACAG AGGGAAATTT 
GAGGAGCTGG AAAGAGAGCA GGGTGAGGCT AAATGGTTGG ACATTATAGA GAAGGATCTC 
CACAGACAGT TCCCCTTCCA TGAAATGTTT GCTGCTCGGG GTGGCCATGG CCAACAGGAC 
CTGTACCGCA TCCTGAAAGC CTACACCATC TATCGGCCTG ATGAGGGATA TTGCCAGGCC 
CAAGCTCCTG TCGCTGCAGT CCTGCTCATG CACATGCCTG CTGAGCAAGC GTTTTGGTGC 
CTCGTACAGA TATGTGAGAA ATATCTCCCT GGCTACTACA GCGCGGGGCT GGAAGCGATC 
CAGCTGGATG GCGAGATCTT CTTCTCTTTA CTACGACGCA CATGTCCAAT GGCGTACCGT 
CATCTCAAGA AGTTCAAGAT CGACCCGATT CTCTACATGA CCGAGTGGTT CATGTGCATC 
TTCTCACGCA CTTTACCATG GGCCTGTGTA CTGCGTGTAT GGGACATGTT CTTCTGTGAA 
GGAGTGAAAA TAGTGTTTCG GGTGGGCTTG GTGCTTCTGA AACAAATGCT TGGCTCAGTG 
GATAAACTTC GAGAACTGCA GGGCATGTAT GAAACAATGG AGCGCCTGAG AAACATTTCT 
CCTGAAAGTG TCAGAGAGGA CATAATAGTA CAGGAGATTG TTATACTCCC AGTGACGGAG 
TCGCTCATTG AGAGGGAGTG CAGCATCCAG GTGAGGAAGT GGAGGGAGTC CAGGGGGGAG 
CTGACACACC AGCCGGGCCG CCGGCTCCAT GGTACGAGAG CCATCTTTGA GTGCAAACGA 
CGCGCTGCCT CCATCAGCTC AGGTGGCAGC TTCTCTTTCC TTGGAAGCTC GATCCCTCCA 
CCAGGACCAC TCAGGGCCTC ATCCAGCCTC CTCTCGCTCC CGGGCTTCCG CAAGTCTAGG 
GCTCCTTTCC ACTCTCAAAC CAAGAAGGGT TCCTTCTCAG GGCCCTCAGG AATGGAGAGC 
CTGCTGCCTC AGTCGCCACC TGTAGTAACA TCAAGCCCTG GTCATGGCCC CAACCACAAA 
CCCCCCATTC CCCCAGGAGC AGGTCAGAGG GCACCACCAG CCCACGTTCA GAGCCCCTTG 
GTTGTGAGTG CGGCTGGCCC ATCACGTGAG CTTGCTCAGC CCTCAGAAGG CTCCTTAGCA 
CAAGGCCAGC CTGCAGCTTC AGCCCCAGTA CAGAGCACAC CACCACCTCC ACCCACCACT 
CTATCCCCCT CCCCGAAGAT CGTCTCGGAG CAAATTACTC CCACTATCCC TTCACCTACT 
GCAAACAACT CCCCTCTGCC TCCATGTCCA GAGACAAAAA AAGAAGCGGT GCCAGTGGCT 
GACGCCACAA CTGGAGAGGA GGAGGGAAAA AAGAAGAAGA AAAGCAAAGA AAACAACAAG 
AAAAAGGAAA AGGAAGATGA AAAGAAAAAA ATGAAGGAAA AGAAGGAAAA GGATAAGGCT 
GAAAAAGAGA GGCTCAGAAG GGAGAAGGAG AGGCTGGAAA AAGAGAAGAA GAAAGGGGGG 
AAGAAAAAGG ACAAAGGGGG AGGAGAGGCT GAAGACAAAA ATGGAGCTGC AGCAGCGAAA 
GATTCGGCCT AG