gfm1 cDNA ORF clone, Paralichthys olivaceus(Japanese flounder)

Gene
Clones
gRNAs

Summary

Gene Symbol	gfm1
Entrez Gene ID	109630332
Full Name	G elongation factor mitochondrial 1
Gene Type	protein-coding
Organism	Paralichthys olivaceus(Japanese flounder)

mRNA and Protein(s)

mRNA	Protein	Name
XM_020088475.1	XP_019944034.1	elongation factor G, mitochondrial

Pathways from BioSystems
Gene Ontology

The following gfm1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gfm1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPa241541	XM_020088475.1 Latest version!	Paralichthys olivaceus G elongation factor mitochondrial 1 (gfm1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

gfm1 ( XM_020088475.1 ) cDNA ORF clone, Paralichthys olivaceus(Japanese flounder) -> XP_019944034.1 Paralichthys olivaceus G elongation factor mitochondrial 1 (gfm1), mRNA.

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CloneID	OPa241541
Clone ID Related Accession (Same CDS sequence)	XM_020088475.1
Accession Version	XM_020088475.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2247bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-07
Organism	Paralichthys olivaceus(Japanese flounder)
Product	elongation factor G, mitochondrial
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017859654.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Paralichthys olivaceus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGACTTT TAAAAGCGGG TCTGTGTTTG GCCAGGACGC ATCTTTTGTC ACCAGGAGGC 
GTCACTGTGA AGAAGGTGCT CATCAACAGC TGTCGGACAT GCTCCTCCGG TATCATCCCC 
AACGAGATGA TCCGCAACAT CGGGATCTCT GCTCACATCG ACTCGGGGAA GACCACCCTG 
ACCGAGCGGG TCCTCTTCTA CACGGGCAGG ATAGCGGAGA TTCACGAGGT GAAGGGGAAG 
GACGGTGTTG GAGCCACCAT GGACTCCATG GAGCTGGAGA GACAGAGAGG CATCACCATC 
CAGTCAGCTG CCACATACAC AATGTGGAAG AACCACAACA TCAACATCAT TGACACACCA 
GGTCATGTGG ACTTTACCAT CGAAGTGGAG CGAGCGCTAC GTGTGCTGGA TGGGGCCGTG 
CTTGTTCTGT GTGCCGTGGG AGGGGTCCAG TGCCAGACCA TGACCGTGAA CAGACAGATG 
AAACGCTACA ACGTGCCCTT CCTCACCTTC ATCAACAAGC TGGACCGCAC GGGTGCCAAC 
CCCATCCGAG CCCTGCAGCA GCTGAGAACT AAACTGAACC ACAACGCAGC CTTTGTGAGC 
ATCCCCATTG GCCTGGAGGG AAACATGCGT GGCATAATTG ACCTTGTGCA GGAGCAGAGC 
ATGTACTTTG AAGGGCCATT TGGTCAAAAT ATCCGCTATG ATGAGATCCC TGCTGATTTT 
CGTGCCGAGG CTTCAGACCG GAGGCAGGAA CTGGTGGAGT GTGTGGCCAA CGCTGACGAA 
ATCTTGGGAG AGATGTTTTT GGAGGAAAAA ATCCCCACCA ATGACGACTT GAAGGCAGCA 
ATCCGCAGAG CCACGGTGCA GCGTCTCTTC ACCCCAGTGT TGGTGGGCAC GGCGCTGAAG 
AACAAGGGTG TTCAGCCTCT GCTGGACGCT GCGCTGGATT ACCTACCCAA CCCCACTGAG 
GTCAAAAACT ATGCCATCCT CAATGACGAA GAAACTAGTG AAACAACAAA GCTTCAAATG 
GACCCGACAA GAAGTGCTGT AAATCCTTTC GTTGGTCTGG CCTTCAAACT GGAGGCGGGA 
AGGTTTGGTC AGCTGACGTA TGTGCGGGTG TACCAGGGCT GTCTCAAGAA AGGAGAATAC 
ATCTACAACA CACGCACCAG CAAAAGGGTC AGAGTTCAGA GGCTTGTGCG TCTCCATGCC 
GACCAGATGG AGGATGTGGA TGAAGTGTAT GCAGGAGATA TTTGTGCTCT TTTTGGGATC 
GACTGTGCCA GTGGAGACAC TTTCACATCC AGGACCAGCG CTAACCTCTC AATGGAGTCC 
ATTCACATCC CAGAGCCTGT CATTTCCATG TCAATCAAGC CAGCAATCAA GAACGACCTG 
GACAAATTCT CCAAAGGCAT CAACCGTTTC ACCAGAGAGG ACCCGACGTT CAGAGTTCAT 
TATGACACAG AGAGCAAAGA AACCATCATC TCAGGAATGG GCGAGCTGCA TCTTGAAATC 
TACTCGCAGA GGATGGAGAG AGAATACAAC TGTCCCTGTG TGATGGGGAA ACCCAAAGTG 
GCTTTCAGAG AGACAATCAC CGATGCTATA CCGTTTGATT TCACCCATAA GAAGCAGAGT 
GGTGGCTCTG GACAGTACGG TAAAGTTATC GGGGTCCTCG AGCCTCTGGA GCCGGAACAT 
TACACCAAGC TGGAATTTGA CGATCAGACA GTCGGCACAA ATGTCCCGAA GCAGTTTGTG 
CCCGCCGTCG AAAAGGGCTT CAGGGAGGCC TGTGAGAAGG GACCCCTGAG TGGGCACAAG 
ATCTCAGGAG TCAGGTTCCT CCTGGAGGAC GGAGCTCATC ACATGGTCGA CTCCAATGAG 
ATCTCCTTCA TCCGTGCAGG AGACGGCGCT GTGAAGCAAG CGATGGAAAA GGCCAACGCG 
ACCATCCTTG AGCCAATCAT GTCTGTGGAA GTAGTAGCAC CTCAGGAGTT TCAGGGAGCA 
GTCATTTCTG GTGTAAACCG TCGTCATGGT GTCATCACAG GACAGGACGG GGCAGAGGGA 
TACTTCACTC TGTACGCTGA TATTCCACTG AATGACATGT TTGGCTACGC TACAGAACTT 
CGCTCCTGCA CTGAAGGGAA AGGCGAGTAC ACCATGGACT ACAGCAGATA CCAGCCCTGC 
CTGCCGGCCA CACAAGAAGA GCTCATCCAC AAATACTTAG AGGCTACAGG ACAGCTGCCT 
GCCAAGAAGA ACAAGTGGAA GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019944034.1
CDS	157..2403
Translation	MRLLKAGLCLARTHLLSPGGVTVKKVLINSCRTCSSGIIPNEMIRNIGISAHIDSGKTTLTERVLFYTGRIAEIHEVKGKDGVGATMDSMELERQRGITIQSAATYTMWKNHNINIIDTPGHVDFTIEVERALRVLDGAVLVLCAVGGVQCQTMTVNRQMKRYNVPFLTFINKLDRTGANPIRALQQLRTKLNHNAAFVSIPIGLEGNMRGIIDLVQEQSMYFEGPFGQNIRYDEIPADFRAEASDRRQELVECVANADEILGEMFLEEKIPTNDDLKAAIRRATVQRLFTPVLVGTALKNKGVQPLLDAALDYLPNPTEVKNYAILNDEETSETTKLQMDPTRSAVNPFVGLAFKLEAGRFGQLTYVRVYQGCLKKGEYIYNTRTSKRVRVQRLVRLHADQMEDVDEVYAGDICALFGIDCASGDTFTSRTSANLSMESIHIPEPVISMSIKPAIKNDLDKFSKGINRFTREDPTFRVHYDTESKETIISGMGELHLEIYSQRMEREYNCPCVMGKPKVAFRETITDAIPFDFTHKKQSGGSGQYGKVIGVLEPLEPEHYTKLEFDDQTVGTNVPKQFVPAVEKGFREACEKGPLSGHKISGVRFLLEDGAHHMVDSNEISFIRAGDGAVKQAMEKANATILEPIMSVEVVAPQEFQGAVISGVNRRHGVITGQDGAEGYFTLYADIPLNDMFGYATELRSCTEGKGEYTMDYSRYQPCLPATQEELIHKYLEATGQLPAKKNKWKN

Target ORF information:

RefSeq Version	XM_020088475.1
Organism	Paralichthys olivaceus(Japanese flounder)
Definition	Paralichthys olivaceus G elongation factor mitochondrial 1 (gfm1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020088475.1

ORF Insert Sequence:

ATGAGACTTT TAAAAGCGGG TCTGTGTTTG GCCAGGACGC ATCTTTTGTC ACCAGGAGGC 
GTCACTGTGA AGAAGGTGCT CATCAACAGC TGTCGGACAT GCTCCTCCGG TATCATCCCC 
AACGAGATGA TCCGCAACAT CGGGATCTCT GCTCACATCG ACTCGGGGAA GACCACCCTG 
ACCGAGCGGG TCCTCTTCTA CACGGGCAGG ATAGCGGAGA TTCACGAGGT GAAGGGGAAG 
GACGGTGTTG GAGCCACCAT GGACTCCATG GAGCTGGAGA GACAGAGAGG CATCACCATC 
CAGTCAGCTG CCACATACAC AATGTGGAAG AACCACAACA TCAACATCAT TGACACACCA 
GGTCATGTGG ACTTTACCAT CGAAGTGGAG CGAGCGCTAC GTGTGCTGGA TGGGGCCGTG 
CTTGTTCTGT GTGCCGTGGG AGGGGTCCAG TGCCAGACCA TGACCGTGAA CAGACAGATG 
AAACGCTACA ACGTGCCCTT CCTCACCTTC ATCAACAAGC TGGACCGCAC GGGTGCCAAC 
CCCATCCGAG CCCTGCAGCA GCTGAGAACT AAACTGAACC ACAACGCAGC CTTTGTGAGC 
ATCCCCATTG GCCTGGAGGG AAACATGCGT GGCATAATTG ACCTTGTGCA GGAGCAGAGC 
ATGTACTTTG AAGGGCCATT TGGTCAAAAT ATCCGCTATG ATGAGATCCC TGCTGATTTT 
CGTGCCGAGG CTTCAGACCG GAGGCAGGAA CTGGTGGAGT GTGTGGCCAA CGCTGACGAA 
ATCTTGGGAG AGATGTTTTT GGAGGAAAAA ATCCCCACCA ATGACGACTT GAAGGCAGCA 
ATCCGCAGAG CCACGGTGCA GCGTCTCTTC ACCCCAGTGT TGGTGGGCAC GGCGCTGAAG 
AACAAGGGTG TTCAGCCTCT GCTGGACGCT GCGCTGGATT ACCTACCCAA CCCCACTGAG 
GTCAAAAACT ATGCCATCCT CAATGACGAA GAAACTAGTG AAACAACAAA GCTTCAAATG 
GACCCGACAA GAAGTGCTGT AAATCCTTTC GTTGGTCTGG CCTTCAAACT GGAGGCGGGA 
AGGTTTGGTC AGCTGACGTA TGTGCGGGTG TACCAGGGCT GTCTCAAGAA AGGAGAATAC 
ATCTACAACA CACGCACCAG CAAAAGGGTC AGAGTTCAGA GGCTTGTGCG TCTCCATGCC 
GACCAGATGG AGGATGTGGA TGAAGTGTAT GCAGGAGATA TTTGTGCTCT TTTTGGGATC 
GACTGTGCCA GTGGAGACAC TTTCACATCC AGGACCAGCG CTAACCTCTC AATGGAGTCC 
ATTCACATCC CAGAGCCTGT CATTTCCATG TCAATCAAGC CAGCAATCAA GAACGACCTG 
GACAAATTCT CCAAAGGCAT CAACCGTTTC ACCAGAGAGG ACCCGACGTT CAGAGTTCAT 
TATGACACAG AGAGCAAAGA AACCATCATC TCAGGAATGG GCGAGCTGCA TCTTGAAATC 
TACTCGCAGA GGATGGAGAG AGAATACAAC TGTCCCTGTG TGATGGGGAA ACCCAAAGTG 
GCTTTCAGAG AGACAATCAC CGATGCTATA CCGTTTGATT TCACCCATAA GAAGCAGAGT 
GGTGGCTCTG GACAGTACGG TAAAGTTATC GGGGTCCTCG AGCCTCTGGA GCCGGAACAT 
TACACCAAGC TGGAATTTGA CGATCAGACA GTCGGCACAA ATGTCCCGAA GCAGTTTGTG 
CCCGCCGTCG AAAAGGGCTT CAGGGAGGCC TGTGAGAAGG GACCCCTGAG TGGGCACAAG 
ATCTCAGGAG TCAGGTTCCT CCTGGAGGAC GGAGCTCATC ACATGGTCGA CTCCAATGAG 
ATCTCCTTCA TCCGTGCAGG AGACGGCGCT GTGAAGCAAG CGATGGAAAA GGCCAACGCG 
ACCATCCTTG AGCCAATCAT GTCTGTGGAA GTAGTAGCAC CTCAGGAGTT TCAGGGAGCA 
GTCATTTCTG GTGTAAACCG TCGTCATGGT GTCATCACAG GACAGGACGG GGCAGAGGGA 
TACTTCACTC TGTACGCTGA TATTCCACTG AATGACATGT TTGGCTACGC TACAGAACTT 
CGCTCCTGCA CTGAAGGGAA AGGCGAGTAC ACCATGGACT ACAGCAGATA CCAGCCCTGC 
CTGCCGGCCA CACAAGAAGA GCTCATCCAC AAATACTTAG AGGCTACAGG ACAGCTGCCT 
GCCAAGAAGA ACAAGTGGAA GAACTGA