myom1 cDNA ORF clone, Paralichthys olivaceus(Japanese flounder)

Gene
Clones
gRNAs

Summary

Gene Symbol	myom1
Entrez Gene ID	109629741
Full Name	myomesin 1
Gene Type	protein-coding
Organism	Paralichthys olivaceus(Japanese flounder)

mRNA and Protein(s)

mRNA	Protein	Name
XM_020087596.1	XP_019943155.1	myomesin-1 isoform X2
XM_020087598.1	XP_019943157.1	myomesin-1 isoform X4
XM_020087600.1	XP_019943159.1	myomesin-1 isoform X5
XM_020087602.1	XP_019943161.1	myomesin-1 isoform X6
XM_020087597.1	XP_019943156.1	myomesin-1 isoform X3
XM_020087601.1	XP_019943160.1	myomesin-1 isoform X5
XM_020087595.1	XP_019943154.1	myomesin-1 isoform X1

Pathways from BioSystems
Gene Ontology

The following myom1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the myom1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPa240840	XM_020087600.1 Latest version!	Paralichthys olivaceus myomesin 1 (myom1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OPa240840	XM_020087601.1 Latest version!	Paralichthys olivaceus myomesin 1 (myom1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OPa240836	XM_020087595.1 Latest version!	Paralichthys olivaceus myomesin 1 (myom1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OPa240837	XM_020087596.1 Latest version!	Paralichthys olivaceus myomesin 1 (myom1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OPa240838	XM_020087597.1 Latest version!	Paralichthys olivaceus myomesin 1 (myom1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OPa240839	XM_020087598.1 Latest version!	Paralichthys olivaceus myomesin 1 (myom1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OPa240841	XM_020087602.1 Latest version!	Paralichthys olivaceus myomesin 1 (myom1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

myom1 ( XM_020087600.1 ) cDNA ORF clone, Paralichthys olivaceus(Japanese flounder) -> XP_019943159.1 Paralichthys olivaceus myomesin 1 (myom1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OPa240840
Clone ID Related Accession (Same CDS sequence)	XM_020087600.1 , XM_020087601.1
Accession Version	XM_020087600.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4239bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-07
Organism	Paralichthys olivaceus(Japanese flounder)
Product	myomesin-1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017859653.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Paralichthys olivaceus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCTGG GGTGCACAGT CGTCATCTAC CCGTCCCTGC ACCTCTACCA GCCAGAGGTC 
CAGTGGTACA AAGATGATGT CCTGCTGTCT CCATCTAAAT GGTACCAAAT GCGCTGGAGC 
GGAGACAGAG CCACACTGAC GCTCACACAC CTGAACAAGG AGGACGAGGG TCTCTACACC 
CTGAGAGTCA CCACCAAGTC TGGATATGAA ACCTACTCCT CCTATGTCTT CGTCAGAGAT 
TCTGATGCTG AGGTGGAAGG AGCTCCAGGA GCCCCTCTGG ATGTTCGCTG TCTCGATGCC 
AACAAGGACT ACATCATTGT CACTTGGAAG CAGCCGGCTG TCGATGGTGG CAACTCCATC 
ATGGGCTACT TTGTGGACAG GTGTGAGGTT GGAACCAACC ACTGGATCCA GTGCAATGAC 
ACTCCAGTCA AGTTCGCCCG TTTCCCCGTG ACCGGTTTGG TGGAGGGTCG TTCTTATTTC 
TTCCGCGTGC GTGCGGTCAA CAACAGCGGC ATGAGTCATC CGTCCAGAGT GTCTGAACCC 
GTGGCCGCCA TGGACCCGGC TGACCGCGCC CGCTTGAGAG GTACTTCTGC TCCCTGGACT 
GGCCAAATCA TTGTCACAGA GGAGGAACCT GCAGAGGGCA TTGTTCCTGG CAGACCTCTT 
GAACTCCAGG TGACGGAGGC CACCAAGAAT TACGTAGTGC TGAGCTGGAA GCCACCTGGA 
GAGAAAGGCC TGGAGGGAGT GATGTACTAC GTGGAGAAGT GTGTCTCGGG CACAGACAGC 
TGGCAGAGGG TGAACACAGA GATCCCAGTC AAATCCCCTC GCTTTGCGTT GTTCGATCTG 
GCCGAGGGGA AGTCCTACAG CTTCCGTGTC CGGTGCTGCA ACCCTGCCGG GGTCGGTGAA 
CCCTCTGACC CCACTGAGGC CACCACAGTT GGCGACAAGC TTGATATCCC ATCAGCCCCC 
AGCAAAGTCG TCCCCACTAG AAACACAGAC ACGTCAGTCG TCGTGTCTTG GGCAGCGTCC 
AGTGATGCCA AAGAGTTGGT TGGTTACTAC ATTGAGGGGA GCATCGTGGG CAGCAACGTG 
TGGGAGCCGT GCAACAACAA GCCTGTAAAT GTAACCAGGT TCATCTGCCA CGGTCTGATG 
ACGGGAGAGA AGTACGTGTT CAGGGTGAGG GCAGTGAACG CAGCAGGACT CAGCCAGTTC 
TCCCCAGAGT CTGAGCCTGT TGAGGTGAAG GCTGCTATTG GGGGCGGCAT CCCTCATGGT 
GTGTTGCCGG AGATGGGGCC GGGGGGTAAC GTTGGCCAAC TAACTGAACA CAGGCCACAC 
TGGACGGGCA CGCATGAAAC TGTCCAGCCC TCCCCTGACA CTAAGCAAAA GTGCAATAAA 
AAAGCTCAGA CTGACACTGG AGCCAGGGAG GGGGCCGGGG CTGCTGGGTG CGGAGGCCGC 
ACAGAGCCCG ACCCCTCCGA GAGTAAAAAC AAGGGGTGCA AGAGAGGCAG TGTGTCCTGG 
GCCCCTGACC TGGCAAAAGA CCTGGTCTCA GAGTCCGTGT CAGGCTCGGT TTCTAACTCA 
CACACTGACA AAGACACAGC CACATGCTTA GAGCCAGTCA CTGCTCAGGT TCAGGATGTA 
GAGACAGCTG ACGGGCCTGA GGCCGCGCAG GCACGTAAGT TCTCTGTAGA CCCTAATCGG 
GAGACAGCGA GGCCGAGGAG AGAGTCGACA GCCTCCCCTG CTCCACCCTA TGGCATCAGC 
GTCTTGGAGT GTGTGCGCGA CTCCATGGTG CTGGGCTGGA AACAGCCGAG CTTCATCGGC 
GGTGCTGACA TCACCGGCTA CTTCGTGGAT TACCGCGAGG TCATCGATGG CGTGCCGGAG 
AAGTGGCACG AGGCCAACAT CAAGGCTGTC AGTGAGAGGG CCTACAGAGT GTCCGACCTG 
ATGGAGAACA GGAAGTACCA GTTCCAGGTG CGAGCAGCCA ACATGGCAGG TGTTGGGATC 
CCGTCGCTGG CCAGTGACAC CTTCCTGTGT GAGGAGTGGA CCATCGCTGT GCCAGGACCT 
CCTCATGACC TGCAGGTGAG AGAGGTGCGC AACGACTCTC TGGTATTGCT ATGGAAACCA 
CCTGTGTACC AGGGCCGCGA TCCGGTCAAC GGGTTCTACA TTGACATCAA GGAAGCGGAC 
GCCCCAGAGG AGGCGTGGAG AGGCGTCAAC AACAAGGCCA GGGAGAAGAC GTACATGAAG 
ATTACGAACC TGAAGGAGGG AGAGGTGTAC ATCTTCCGTG TTCGTGCTCA GAATAAAGCC 
GGTGTTGGAA AAACATCTGA CGTGACAGAA CCGGTTTCAG CTGTGACCAA ACCTGGCACT 
AAGGAGATCG TCGTGGACGT AGACGATGAT GGCATCATCT CCCTGAACTT TGAATGCTGT 
GACCTGACCC CTGACTCCAA ATTTGTGTGG TCCAAGAATT ACGAGGAGAT CACAGACCCC 
TCACGCTTGA CTATGGAAAC CAAGGGAAAC AAATCGAAAG CTGTTTTCAA CACTCCTGGA 
GAGGAGGACA TCGGCATTTA CTCCTGTATC GTCACACACA CTGATGGAGC TTCATCCAGC 
TACGATCTCT CTGAGGAAGA GTTGAAGAGG CTGCTGGAGG TCAGTCACGA CCACAAATTC 
CCCATTATTC CCCTGAAGTC ACAGTTGGCT GTGGAACTGC TGGAGAAAGG CAGAGTTCGC 
TTTTGGCTGC AGGCCGAGAA GATTTCTGCT GATGGCAAAG TCGATTACGT GTTTAATGAA 
AATCTCATCT CTCAGGGGGA GAAATATAAG ATGAACTTCG ACAAGAGCAC CGGTGTGATC 
GAGATGATCA TGGAGTCTCT GACCCCAGCG GACGAGGGCA CCTTCACCTT CCAGCTGAAA 
GACGGGAAAG CAACCAACCA GTCCAGCTTG GTGCTGATAG GAAACGTGTT CAAGGAGCTT 
CAGAAGGAAT CAGAGTTCCA GAGGAAAGAA TGGTTCAGAA AGCAAGGTCC TCACTTCATC 
GAGTATTTGA GTTATGAGGT GACGCCCGAG TGCTGCGTTG TGCTGAAATG CAAGGTCGGC 
AACGTGAAGA AGGAAACCTC GGCGCTGTGG TACAAAGACG GACGAGAGAT CAAGGCAGAA 
GAGCATCTGA GCTTCACAGA GGGAGTGTTG AAACTGGAAA TAGCTCAGAT TTCCAAGAGG 
GATTCTGGTG TGTACGAGGT CGTTCTGAAG GACGACAGAG GAAAAGACAC GTCCACATTG 
AATTTAACAG ATCAAGGTTT CAAGGACTTG ATGAATGAAG TTTTCAGTTT TATTGCCAAT 
TCCTCCACTC CACTGAAGAT CACGAGCACA GATGAGGGCA TCCGACTCTA CACCTTCGTC 
AACTTCTATA ACGATTTACT CCAAGTGACA TGGCACTACA AAGAATCAGC CATCGCCTTC 
TCTGACCGTA TAAAGAGCGG CGTGGTGGGG GAGCAGCTGT GGCTGCAGAT CACTGAGCCC 
ACAGAGAAAG ACACGGGCAA GTACGCCATC GAGTTCCACG ACGGAAAGGG AGGCCTGAGG 
AGAACTGTAG AGTTGTCTGG TCAAGCATTT GATGACGCTT TTGCAGAATT CCAGAGACTC 
AAAGCCGCTG CTGTTGCAGA GAGAGGTCGT GCTCGAGTAG CAGGAGGTTT GCCTGATGTG 
GTCACCATCC AGGAGGGCAA GGCCCTCAAT CTCACCTGCA ACATTTCGGG AGACCCCGTG 
CCAAAGGTCA CCTGGCTGAA GAACGACAGG GAAATCACGT CCGACGACCA CTGCATCCTG 
AAGTTCGAGT CCGGCAAGTT CGCCAGTTTC ACCATCACGG CTGTGAACAC GTCGGACACC 
GGAAAATACA GCATCCTGGT GAAGAACAAG TACGGCACAG AGAGCGGGGA CTTCACCCTG 
GAGACCACGG AGGGGACGCC TGACCTGGGC GTGACCTCAT ACTGGACCGA CGCAGATGGA 
AATGTGGTGT TTGGCCCAAA CACTCCCAAG GAAAAACGAA AGGAGTCTAT GGCTAAAGCT 
GCGAAAATGA AGGAAGACAA AAAAGAGAGA AAAAGCGAGG GATCGGAGGA GCAGAAAAAT 
GAGATTAAAG TTGATGCCGC TGCAGAAACA AAAGCTTCTG ATTCAAAACA AACAGGAGAC 
GTAAAGAAAG ATTCAGCCGA CCAATCAGAA ACCAGTGAGC GGCCTGCAGA CACAACGGAG 
GAGCCGGAAC CAGAGGCTGA GACGACACAG CCGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019943159.1
CDS	168..4406
Translation	MSLGCTVVIYPSLHLYQPEVQWYKDDVLLSPSKWYQMRWSGDRATLTLTHLNKEDEGLYTLRVTTKSGYETYSSYVFVRDSDAEVEGAPGAPLDVRCLDANKDYIIVTWKQPAVDGGNSIMGYFVDRCEVGTNHWIQCNDTPVKFARFPVTGLVEGRSYFFRVRAVNNSGMSHPSRVSEPVAAMDPADRARLRGTSAPWTGQIIVTEEEPAEGIVPGRPLELQVTEATKNYVVLSWKPPGEKGLEGVMYYVEKCVSGTDSWQRVNTEIPVKSPRFALFDLAEGKSYSFRVRCCNPAGVGEPSDPTEATTVGDKLDIPSAPSKVVPTRNTDTSVVVSWAASSDAKELVGYYIEGSIVGSNVWEPCNNKPVNVTRFICHGLMTGEKYVFRVRAVNAAGLSQFSPESEPVEVKAAIGGGIPHGVLPEMGPGGNVGQLTEHRPHWTGTHETVQPSPDTKQKCNKKAQTDTGAREGAGAAGCGGRTEPDPSESKNKGCKRGSVSWAPDLAKDLVSESVSGSVSNSHTDKDTATCLEPVTAQVQDVETADGPEAAQARKFSVDPNRETARPRRESTASPAPPYGISVLECVRDSMVLGWKQPSFIGGADITGYFVDYREVIDGVPEKWHEANIKAVSERAYRVSDLMENRKYQFQVRAANMAGVGIPSLASDTFLCEEWTIAVPGPPHDLQVREVRNDSLVLLWKPPVYQGRDPVNGFYIDIKEADAPEEAWRGVNNKAREKTYMKITNLKEGEVYIFRVRAQNKAGVGKTSDVTEPVSAVTKPGTKEIVVDVDDDGIISLNFECCDLTPDSKFVWSKNYEEITDPSRLTMETKGNKSKAVFNTPGEEDIGIYSCIVTHTDGASSSYDLSEEELKRLLEVSHDHKFPIIPLKSQLAVELLEKGRVRFWLQAEKISADGKVDYVFNENLISQGEKYKMNFDKSTGVIEMIMESLTPADEGTFTFQLKDGKATNQSSLVLIGNVFKELQKESEFQRKEWFRKQGPHFIEYLSYEVTPECCVVLKCKVGNVKKETSALWYKDGREIKAEEHLSFTEGVLKLEIAQISKRDSGVYEVVLKDDRGKDTSTLNLTDQGFKDLMNEVFSFIANSSTPLKITSTDEGIRLYTFVNFYNDLLQVTWHYKESAIAFSDRIKSGVVGEQLWLQITEPTEKDTGKYAIEFHDGKGGLRRTVELSGQAFDDAFAEFQRLKAAAVAERGRARVAGGLPDVVTIQEGKALNLTCNISGDPVPKVTWLKNDREITSDDHCILKFESGKFASFTITAVNTSDTGKYSILVKNKYGTESGDFTLETTEGTPDLGVTSYWTDADGNVVFGPNTPKEKRKESMAKAAKMKEDKKERKSEGSEEQKNEIKVDAAAETKASDSKQTGDVKKDSADQSETSERPADTTEEPEPEAETTQPK

Target ORF information:

RefSeq Version	XM_020087600.1
Organism	Paralichthys olivaceus(Japanese flounder)
Definition	Paralichthys olivaceus myomesin 1 (myom1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020087600.1

ORF Insert Sequence:

ATGAGTCTGG GGTGCACAGT CGTCATCTAC CCGTCCCTGC ACCTCTACCA GCCAGAGGTC 
CAGTGGTACA AAGATGATGT CCTGCTGTCT CCATCTAAAT GGTACCAAAT GCGCTGGAGC 
GGAGACAGAG CCACACTGAC GCTCACACAC CTGAACAAGG AGGACGAGGG TCTCTACACC 
CTGAGAGTCA CCACCAAGTC TGGATATGAA ACCTACTCCT CCTATGTCTT CGTCAGAGAT 
TCTGATGCTG AGGTGGAAGG AGCTCCAGGA GCCCCTCTGG ATGTTCGCTG TCTCGATGCC 
AACAAGGACT ACATCATTGT CACTTGGAAG CAGCCGGCTG TCGATGGTGG CAACTCCATC 
ATGGGCTACT TTGTGGACAG GTGTGAGGTT GGAACCAACC ACTGGATCCA GTGCAATGAC 
ACTCCAGTCA AGTTCGCCCG TTTCCCCGTG ACCGGTTTGG TGGAGGGTCG TTCTTATTTC 
TTCCGCGTGC GTGCGGTCAA CAACAGCGGC ATGAGTCATC CGTCCAGAGT GTCTGAACCC 
GTGGCCGCCA TGGACCCGGC TGACCGCGCC CGCTTGAGAG GTACTTCTGC TCCCTGGACT 
GGCCAAATCA TTGTCACAGA GGAGGAACCT GCAGAGGGCA TTGTTCCTGG CAGACCTCTT 
GAACTCCAGG TGACGGAGGC CACCAAGAAT TACGTAGTGC TGAGCTGGAA GCCACCTGGA 
GAGAAAGGCC TGGAGGGAGT GATGTACTAC GTGGAGAAGT GTGTCTCGGG CACAGACAGC 
TGGCAGAGGG TGAACACAGA GATCCCAGTC AAATCCCCTC GCTTTGCGTT GTTCGATCTG 
GCCGAGGGGA AGTCCTACAG CTTCCGTGTC CGGTGCTGCA ACCCTGCCGG GGTCGGTGAA 
CCCTCTGACC CCACTGAGGC CACCACAGTT GGCGACAAGC TTGATATCCC ATCAGCCCCC 
AGCAAAGTCG TCCCCACTAG AAACACAGAC ACGTCAGTCG TCGTGTCTTG GGCAGCGTCC 
AGTGATGCCA AAGAGTTGGT TGGTTACTAC ATTGAGGGGA GCATCGTGGG CAGCAACGTG 
TGGGAGCCGT GCAACAACAA GCCTGTAAAT GTAACCAGGT TCATCTGCCA CGGTCTGATG 
ACGGGAGAGA AGTACGTGTT CAGGGTGAGG GCAGTGAACG CAGCAGGACT CAGCCAGTTC 
TCCCCAGAGT CTGAGCCTGT TGAGGTGAAG GCTGCTATTG GGGGCGGCAT CCCTCATGGT 
GTGTTGCCGG AGATGGGGCC GGGGGGTAAC GTTGGCCAAC TAACTGAACA CAGGCCACAC 
TGGACGGGCA CGCATGAAAC TGTCCAGCCC TCCCCTGACA CTAAGCAAAA GTGCAATAAA 
AAAGCTCAGA CTGACACTGG AGCCAGGGAG GGGGCCGGGG CTGCTGGGTG CGGAGGCCGC 
ACAGAGCCCG ACCCCTCCGA GAGTAAAAAC AAGGGGTGCA AGAGAGGCAG TGTGTCCTGG 
GCCCCTGACC TGGCAAAAGA CCTGGTCTCA GAGTCCGTGT CAGGCTCGGT TTCTAACTCA 
CACACTGACA AAGACACAGC CACATGCTTA GAGCCAGTCA CTGCTCAGGT TCAGGATGTA 
GAGACAGCTG ACGGGCCTGA GGCCGCGCAG GCACGTAAGT TCTCTGTAGA CCCTAATCGG 
GAGACAGCGA GGCCGAGGAG AGAGTCGACA GCCTCCCCTG CTCCACCCTA TGGCATCAGC 
GTCTTGGAGT GTGTGCGCGA CTCCATGGTG CTGGGCTGGA AACAGCCGAG CTTCATCGGC 
GGTGCTGACA TCACCGGCTA CTTCGTGGAT TACCGCGAGG TCATCGATGG CGTGCCGGAG 
AAGTGGCACG AGGCCAACAT CAAGGCTGTC AGTGAGAGGG CCTACAGAGT GTCCGACCTG 
ATGGAGAACA GGAAGTACCA GTTCCAGGTG CGAGCAGCCA ACATGGCAGG TGTTGGGATC 
CCGTCGCTGG CCAGTGACAC CTTCCTGTGT GAGGAGTGGA CCATCGCTGT GCCAGGACCT 
CCTCATGACC TGCAGGTGAG AGAGGTGCGC AACGACTCTC TGGTATTGCT ATGGAAACCA 
CCTGTGTACC AGGGCCGCGA TCCGGTCAAC GGGTTCTACA TTGACATCAA GGAAGCGGAC 
GCCCCAGAGG AGGCGTGGAG AGGCGTCAAC AACAAGGCCA GGGAGAAGAC GTACATGAAG 
ATTACGAACC TGAAGGAGGG AGAGGTGTAC ATCTTCCGTG TTCGTGCTCA GAATAAAGCC 
GGTGTTGGAA AAACATCTGA CGTGACAGAA CCGGTTTCAG CTGTGACCAA ACCTGGCACT 
AAGGAGATCG TCGTGGACGT AGACGATGAT GGCATCATCT CCCTGAACTT TGAATGCTGT 
GACCTGACCC CTGACTCCAA ATTTGTGTGG TCCAAGAATT ACGAGGAGAT CACAGACCCC 
TCACGCTTGA CTATGGAAAC CAAGGGAAAC AAATCGAAAG CTGTTTTCAA CACTCCTGGA 
GAGGAGGACA TCGGCATTTA CTCCTGTATC GTCACACACA CTGATGGAGC TTCATCCAGC 
TACGATCTCT CTGAGGAAGA GTTGAAGAGG CTGCTGGAGG TCAGTCACGA CCACAAATTC 
CCCATTATTC CCCTGAAGTC ACAGTTGGCT GTGGAACTGC TGGAGAAAGG CAGAGTTCGC 
TTTTGGCTGC AGGCCGAGAA GATTTCTGCT GATGGCAAAG TCGATTACGT GTTTAATGAA 
AATCTCATCT CTCAGGGGGA GAAATATAAG ATGAACTTCG ACAAGAGCAC CGGTGTGATC 
GAGATGATCA TGGAGTCTCT GACCCCAGCG GACGAGGGCA CCTTCACCTT CCAGCTGAAA 
GACGGGAAAG CAACCAACCA GTCCAGCTTG GTGCTGATAG GAAACGTGTT CAAGGAGCTT 
CAGAAGGAAT CAGAGTTCCA GAGGAAAGAA TGGTTCAGAA AGCAAGGTCC TCACTTCATC 
GAGTATTTGA GTTATGAGGT GACGCCCGAG TGCTGCGTTG TGCTGAAATG CAAGGTCGGC 
AACGTGAAGA AGGAAACCTC GGCGCTGTGG TACAAAGACG GACGAGAGAT CAAGGCAGAA 
GAGCATCTGA GCTTCACAGA GGGAGTGTTG AAACTGGAAA TAGCTCAGAT TTCCAAGAGG 
GATTCTGGTG TGTACGAGGT CGTTCTGAAG GACGACAGAG GAAAAGACAC GTCCACATTG 
AATTTAACAG ATCAAGGTTT CAAGGACTTG ATGAATGAAG TTTTCAGTTT TATTGCCAAT 
TCCTCCACTC CACTGAAGAT CACGAGCACA GATGAGGGCA TCCGACTCTA CACCTTCGTC 
AACTTCTATA ACGATTTACT CCAAGTGACA TGGCACTACA AAGAATCAGC CATCGCCTTC 
TCTGACCGTA TAAAGAGCGG CGTGGTGGGG GAGCAGCTGT GGCTGCAGAT CACTGAGCCC 
ACAGAGAAAG ACACGGGCAA GTACGCCATC GAGTTCCACG ACGGAAAGGG AGGCCTGAGG 
AGAACTGTAG AGTTGTCTGG TCAAGCATTT GATGACGCTT TTGCAGAATT CCAGAGACTC 
AAAGCCGCTG CTGTTGCAGA GAGAGGTCGT GCTCGAGTAG CAGGAGGTTT GCCTGATGTG 
GTCACCATCC AGGAGGGCAA GGCCCTCAAT CTCACCTGCA ACATTTCGGG AGACCCCGTG 
CCAAAGGTCA CCTGGCTGAA GAACGACAGG GAAATCACGT CCGACGACCA CTGCATCCTG 
AAGTTCGAGT CCGGCAAGTT CGCCAGTTTC ACCATCACGG CTGTGAACAC GTCGGACACC 
GGAAAATACA GCATCCTGGT GAAGAACAAG TACGGCACAG AGAGCGGGGA CTTCACCCTG 
GAGACCACGG AGGGGACGCC TGACCTGGGC GTGACCTCAT ACTGGACCGA CGCAGATGGA 
AATGTGGTGT TTGGCCCAAA CACTCCCAAG GAAAAACGAA AGGAGTCTAT GGCTAAAGCT 
GCGAAAATGA AGGAAGACAA AAAAGAGAGA AAAAGCGAGG GATCGGAGGA GCAGAAAAAT 
GAGATTAAAG TTGATGCCGC TGCAGAAACA AAAGCTTCTG ATTCAAAACA AACAGGAGAC 
GTAAAGAAAG ATTCAGCCGA CCAATCAGAA ACCAGTGAGC GGCCTGCAGA CACAACGGAG 
GAGCCGGAAC CAGAGGCTGA GACGACACAG CCGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myom1 ( XM_020087601.1 ) cDNA ORF clone, Paralichthys olivaceus(Japanese flounder) -> XP_019943160.1 Paralichthys olivaceus myomesin 1 (myom1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OPa240840
Clone ID Related Accession (Same CDS sequence)	XM_020087600.1 , XM_020087601.1
Accession Version	XM_020087601.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4239bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-07
Organism	Paralichthys olivaceus(Japanese flounder)
Product	myomesin-1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017859653.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Paralichthys olivaceus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCTGG GGTGCACAGT CGTCATCTAC CCGTCCCTGC ACCTCTACCA GCCAGAGGTC 
CAGTGGTACA AAGATGATGT CCTGCTGTCT CCATCTAAAT GGTACCAAAT GCGCTGGAGC 
GGAGACAGAG CCACACTGAC GCTCACACAC CTGAACAAGG AGGACGAGGG TCTCTACACC 
CTGAGAGTCA CCACCAAGTC TGGATATGAA ACCTACTCCT CCTATGTCTT CGTCAGAGAT 
TCTGATGCTG AGGTGGAAGG AGCTCCAGGA GCCCCTCTGG ATGTTCGCTG TCTCGATGCC 
AACAAGGACT ACATCATTGT CACTTGGAAG CAGCCGGCTG TCGATGGTGG CAACTCCATC 
ATGGGCTACT TTGTGGACAG GTGTGAGGTT GGAACCAACC ACTGGATCCA GTGCAATGAC 
ACTCCAGTCA AGTTCGCCCG TTTCCCCGTG ACCGGTTTGG TGGAGGGTCG TTCTTATTTC 
TTCCGCGTGC GTGCGGTCAA CAACAGCGGC ATGAGTCATC CGTCCAGAGT GTCTGAACCC 
GTGGCCGCCA TGGACCCGGC TGACCGCGCC CGCTTGAGAG GTACTTCTGC TCCCTGGACT 
GGCCAAATCA TTGTCACAGA GGAGGAACCT GCAGAGGGCA TTGTTCCTGG CAGACCTCTT 
GAACTCCAGG TGACGGAGGC CACCAAGAAT TACGTAGTGC TGAGCTGGAA GCCACCTGGA 
GAGAAAGGCC TGGAGGGAGT GATGTACTAC GTGGAGAAGT GTGTCTCGGG CACAGACAGC 
TGGCAGAGGG TGAACACAGA GATCCCAGTC AAATCCCCTC GCTTTGCGTT GTTCGATCTG 
GCCGAGGGGA AGTCCTACAG CTTCCGTGTC CGGTGCTGCA ACCCTGCCGG GGTCGGTGAA 
CCCTCTGACC CCACTGAGGC CACCACAGTT GGCGACAAGC TTGATATCCC ATCAGCCCCC 
AGCAAAGTCG TCCCCACTAG AAACACAGAC ACGTCAGTCG TCGTGTCTTG GGCAGCGTCC 
AGTGATGCCA AAGAGTTGGT TGGTTACTAC ATTGAGGGGA GCATCGTGGG CAGCAACGTG 
TGGGAGCCGT GCAACAACAA GCCTGTAAAT GTAACCAGGT TCATCTGCCA CGGTCTGATG 
ACGGGAGAGA AGTACGTGTT CAGGGTGAGG GCAGTGAACG CAGCAGGACT CAGCCAGTTC 
TCCCCAGAGT CTGAGCCTGT TGAGGTGAAG GCTGCTATTG GGGGCGGCAT CCCTCATGGT 
GTGTTGCCGG AGATGGGGCC GGGGGGTAAC GTTGGCCAAC TAACTGAACA CAGGCCACAC 
TGGACGGGCA CGCATGAAAC TGTCCAGCCC TCCCCTGACA CTAAGCAAAA GTGCAATAAA 
AAAGCTCAGA CTGACACTGG AGCCAGGGAG GGGGCCGGGG CTGCTGGGTG CGGAGGCCGC 
ACAGAGCCCG ACCCCTCCGA GAGTAAAAAC AAGGGGTGCA AGAGAGGCAG TGTGTCCTGG 
GCCCCTGACC TGGCAAAAGA CCTGGTCTCA GAGTCCGTGT CAGGCTCGGT TTCTAACTCA 
CACACTGACA AAGACACAGC CACATGCTTA GAGCCAGTCA CTGCTCAGGT TCAGGATGTA 
GAGACAGCTG ACGGGCCTGA GGCCGCGCAG GCACGTAAGT TCTCTGTAGA CCCTAATCGG 
GAGACAGCGA GGCCGAGGAG AGAGTCGACA GCCTCCCCTG CTCCACCCTA TGGCATCAGC 
GTCTTGGAGT GTGTGCGCGA CTCCATGGTG CTGGGCTGGA AACAGCCGAG CTTCATCGGC 
GGTGCTGACA TCACCGGCTA CTTCGTGGAT TACCGCGAGG TCATCGATGG CGTGCCGGAG 
AAGTGGCACG AGGCCAACAT CAAGGCTGTC AGTGAGAGGG CCTACAGAGT GTCCGACCTG 
ATGGAGAACA GGAAGTACCA GTTCCAGGTG CGAGCAGCCA ACATGGCAGG TGTTGGGATC 
CCGTCGCTGG CCAGTGACAC CTTCCTGTGT GAGGAGTGGA CCATCGCTGT GCCAGGACCT 
CCTCATGACC TGCAGGTGAG AGAGGTGCGC AACGACTCTC TGGTATTGCT ATGGAAACCA 
CCTGTGTACC AGGGCCGCGA TCCGGTCAAC GGGTTCTACA TTGACATCAA GGAAGCGGAC 
GCCCCAGAGG AGGCGTGGAG AGGCGTCAAC AACAAGGCCA GGGAGAAGAC GTACATGAAG 
ATTACGAACC TGAAGGAGGG AGAGGTGTAC ATCTTCCGTG TTCGTGCTCA GAATAAAGCC 
GGTGTTGGAA AAACATCTGA CGTGACAGAA CCGGTTTCAG CTGTGACCAA ACCTGGCACT 
AAGGAGATCG TCGTGGACGT AGACGATGAT GGCATCATCT CCCTGAACTT TGAATGCTGT 
GACCTGACCC CTGACTCCAA ATTTGTGTGG TCCAAGAATT ACGAGGAGAT CACAGACCCC 
TCACGCTTGA CTATGGAAAC CAAGGGAAAC AAATCGAAAG CTGTTTTCAA CACTCCTGGA 
GAGGAGGACA TCGGCATTTA CTCCTGTATC GTCACACACA CTGATGGAGC TTCATCCAGC 
TACGATCTCT CTGAGGAAGA GTTGAAGAGG CTGCTGGAGG TCAGTCACGA CCACAAATTC 
CCCATTATTC CCCTGAAGTC ACAGTTGGCT GTGGAACTGC TGGAGAAAGG CAGAGTTCGC 
TTTTGGCTGC AGGCCGAGAA GATTTCTGCT GATGGCAAAG TCGATTACGT GTTTAATGAA 
AATCTCATCT CTCAGGGGGA GAAATATAAG ATGAACTTCG ACAAGAGCAC CGGTGTGATC 
GAGATGATCA TGGAGTCTCT GACCCCAGCG GACGAGGGCA CCTTCACCTT CCAGCTGAAA 
GACGGGAAAG CAACCAACCA GTCCAGCTTG GTGCTGATAG GAAACGTGTT CAAGGAGCTT 
CAGAAGGAAT CAGAGTTCCA GAGGAAAGAA TGGTTCAGAA AGCAAGGTCC TCACTTCATC 
GAGTATTTGA GTTATGAGGT GACGCCCGAG TGCTGCGTTG TGCTGAAATG CAAGGTCGGC 
AACGTGAAGA AGGAAACCTC GGCGCTGTGG TACAAAGACG GACGAGAGAT CAAGGCAGAA 
GAGCATCTGA GCTTCACAGA GGGAGTGTTG AAACTGGAAA TAGCTCAGAT TTCCAAGAGG 
GATTCTGGTG TGTACGAGGT CGTTCTGAAG GACGACAGAG GAAAAGACAC GTCCACATTG 
AATTTAACAG ATCAAGGTTT CAAGGACTTG ATGAATGAAG TTTTCAGTTT TATTGCCAAT 
TCCTCCACTC CACTGAAGAT CACGAGCACA GATGAGGGCA TCCGACTCTA CACCTTCGTC 
AACTTCTATA ACGATTTACT CCAAGTGACA TGGCACTACA AAGAATCAGC CATCGCCTTC 
TCTGACCGTA TAAAGAGCGG CGTGGTGGGG GAGCAGCTGT GGCTGCAGAT CACTGAGCCC 
ACAGAGAAAG ACACGGGCAA GTACGCCATC GAGTTCCACG ACGGAAAGGG AGGCCTGAGG 
AGAACTGTAG AGTTGTCTGG TCAAGCATTT GATGACGCTT TTGCAGAATT CCAGAGACTC 
AAAGCCGCTG CTGTTGCAGA GAGAGGTCGT GCTCGAGTAG CAGGAGGTTT GCCTGATGTG 
GTCACCATCC AGGAGGGCAA GGCCCTCAAT CTCACCTGCA ACATTTCGGG AGACCCCGTG 
CCAAAGGTCA CCTGGCTGAA GAACGACAGG GAAATCACGT CCGACGACCA CTGCATCCTG 
AAGTTCGAGT CCGGCAAGTT CGCCAGTTTC ACCATCACGG CTGTGAACAC GTCGGACACC 
GGAAAATACA GCATCCTGGT GAAGAACAAG TACGGCACAG AGAGCGGGGA CTTCACCCTG 
GAGACCACGG AGGGGACGCC TGACCTGGGC GTGACCTCAT ACTGGACCGA CGCAGATGGA 
AATGTGGTGT TTGGCCCAAA CACTCCCAAG GAAAAACGAA AGGAGTCTAT GGCTAAAGCT 
GCGAAAATGA AGGAAGACAA AAAAGAGAGA AAAAGCGAGG GATCGGAGGA GCAGAAAAAT 
GAGATTAAAG TTGATGCCGC TGCAGAAACA AAAGCTTCTG ATTCAAAACA AACAGGAGAC 
GTAAAGAAAG ATTCAGCCGA CCAATCAGAA ACCAGTGAGC GGCCTGCAGA CACAACGGAG 
GAGCCGGAAC CAGAGGCTGA GACGACACAG CCGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019943160.1
CDS	156..4394
Translation	MSLGCTVVIYPSLHLYQPEVQWYKDDVLLSPSKWYQMRWSGDRATLTLTHLNKEDEGLYTLRVTTKSGYETYSSYVFVRDSDAEVEGAPGAPLDVRCLDANKDYIIVTWKQPAVDGGNSIMGYFVDRCEVGTNHWIQCNDTPVKFARFPVTGLVEGRSYFFRVRAVNNSGMSHPSRVSEPVAAMDPADRARLRGTSAPWTGQIIVTEEEPAEGIVPGRPLELQVTEATKNYVVLSWKPPGEKGLEGVMYYVEKCVSGTDSWQRVNTEIPVKSPRFALFDLAEGKSYSFRVRCCNPAGVGEPSDPTEATTVGDKLDIPSAPSKVVPTRNTDTSVVVSWAASSDAKELVGYYIEGSIVGSNVWEPCNNKPVNVTRFICHGLMTGEKYVFRVRAVNAAGLSQFSPESEPVEVKAAIGGGIPHGVLPEMGPGGNVGQLTEHRPHWTGTHETVQPSPDTKQKCNKKAQTDTGAREGAGAAGCGGRTEPDPSESKNKGCKRGSVSWAPDLAKDLVSESVSGSVSNSHTDKDTATCLEPVTAQVQDVETADGPEAAQARKFSVDPNRETARPRRESTASPAPPYGISVLECVRDSMVLGWKQPSFIGGADITGYFVDYREVIDGVPEKWHEANIKAVSERAYRVSDLMENRKYQFQVRAANMAGVGIPSLASDTFLCEEWTIAVPGPPHDLQVREVRNDSLVLLWKPPVYQGRDPVNGFYIDIKEADAPEEAWRGVNNKAREKTYMKITNLKEGEVYIFRVRAQNKAGVGKTSDVTEPVSAVTKPGTKEIVVDVDDDGIISLNFECCDLTPDSKFVWSKNYEEITDPSRLTMETKGNKSKAVFNTPGEEDIGIYSCIVTHTDGASSSYDLSEEELKRLLEVSHDHKFPIIPLKSQLAVELLEKGRVRFWLQAEKISADGKVDYVFNENLISQGEKYKMNFDKSTGVIEMIMESLTPADEGTFTFQLKDGKATNQSSLVLIGNVFKELQKESEFQRKEWFRKQGPHFIEYLSYEVTPECCVVLKCKVGNVKKETSALWYKDGREIKAEEHLSFTEGVLKLEIAQISKRDSGVYEVVLKDDRGKDTSTLNLTDQGFKDLMNEVFSFIANSSTPLKITSTDEGIRLYTFVNFYNDLLQVTWHYKESAIAFSDRIKSGVVGEQLWLQITEPTEKDTGKYAIEFHDGKGGLRRTVELSGQAFDDAFAEFQRLKAAAVAERGRARVAGGLPDVVTIQEGKALNLTCNISGDPVPKVTWLKNDREITSDDHCILKFESGKFASFTITAVNTSDTGKYSILVKNKYGTESGDFTLETTEGTPDLGVTSYWTDADGNVVFGPNTPKEKRKESMAKAAKMKEDKKERKSEGSEEQKNEIKVDAAAETKASDSKQTGDVKKDSADQSETSERPADTTEEPEPEAETTQPK

Target ORF information:

RefSeq Version	XM_020087601.1
Organism	Paralichthys olivaceus(Japanese flounder)
Definition	Paralichthys olivaceus myomesin 1 (myom1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020087601.1

ORF Insert Sequence:

ATGAGTCTGG GGTGCACAGT CGTCATCTAC CCGTCCCTGC ACCTCTACCA GCCAGAGGTC 
CAGTGGTACA AAGATGATGT CCTGCTGTCT CCATCTAAAT GGTACCAAAT GCGCTGGAGC 
GGAGACAGAG CCACACTGAC GCTCACACAC CTGAACAAGG AGGACGAGGG TCTCTACACC 
CTGAGAGTCA CCACCAAGTC TGGATATGAA ACCTACTCCT CCTATGTCTT CGTCAGAGAT 
TCTGATGCTG AGGTGGAAGG AGCTCCAGGA GCCCCTCTGG ATGTTCGCTG TCTCGATGCC 
AACAAGGACT ACATCATTGT CACTTGGAAG CAGCCGGCTG TCGATGGTGG CAACTCCATC 
ATGGGCTACT TTGTGGACAG GTGTGAGGTT GGAACCAACC ACTGGATCCA GTGCAATGAC 
ACTCCAGTCA AGTTCGCCCG TTTCCCCGTG ACCGGTTTGG TGGAGGGTCG TTCTTATTTC 
TTCCGCGTGC GTGCGGTCAA CAACAGCGGC ATGAGTCATC CGTCCAGAGT GTCTGAACCC 
GTGGCCGCCA TGGACCCGGC TGACCGCGCC CGCTTGAGAG GTACTTCTGC TCCCTGGACT 
GGCCAAATCA TTGTCACAGA GGAGGAACCT GCAGAGGGCA TTGTTCCTGG CAGACCTCTT 
GAACTCCAGG TGACGGAGGC CACCAAGAAT TACGTAGTGC TGAGCTGGAA GCCACCTGGA 
GAGAAAGGCC TGGAGGGAGT GATGTACTAC GTGGAGAAGT GTGTCTCGGG CACAGACAGC 
TGGCAGAGGG TGAACACAGA GATCCCAGTC AAATCCCCTC GCTTTGCGTT GTTCGATCTG 
GCCGAGGGGA AGTCCTACAG CTTCCGTGTC CGGTGCTGCA ACCCTGCCGG GGTCGGTGAA 
CCCTCTGACC CCACTGAGGC CACCACAGTT GGCGACAAGC TTGATATCCC ATCAGCCCCC 
AGCAAAGTCG TCCCCACTAG AAACACAGAC ACGTCAGTCG TCGTGTCTTG GGCAGCGTCC 
AGTGATGCCA AAGAGTTGGT TGGTTACTAC ATTGAGGGGA GCATCGTGGG CAGCAACGTG 
TGGGAGCCGT GCAACAACAA GCCTGTAAAT GTAACCAGGT TCATCTGCCA CGGTCTGATG 
ACGGGAGAGA AGTACGTGTT CAGGGTGAGG GCAGTGAACG CAGCAGGACT CAGCCAGTTC 
TCCCCAGAGT CTGAGCCTGT TGAGGTGAAG GCTGCTATTG GGGGCGGCAT CCCTCATGGT 
GTGTTGCCGG AGATGGGGCC GGGGGGTAAC GTTGGCCAAC TAACTGAACA CAGGCCACAC 
TGGACGGGCA CGCATGAAAC TGTCCAGCCC TCCCCTGACA CTAAGCAAAA GTGCAATAAA 
AAAGCTCAGA CTGACACTGG AGCCAGGGAG GGGGCCGGGG CTGCTGGGTG CGGAGGCCGC 
ACAGAGCCCG ACCCCTCCGA GAGTAAAAAC AAGGGGTGCA AGAGAGGCAG TGTGTCCTGG 
GCCCCTGACC TGGCAAAAGA CCTGGTCTCA GAGTCCGTGT CAGGCTCGGT TTCTAACTCA 
CACACTGACA AAGACACAGC CACATGCTTA GAGCCAGTCA CTGCTCAGGT TCAGGATGTA 
GAGACAGCTG ACGGGCCTGA GGCCGCGCAG GCACGTAAGT TCTCTGTAGA CCCTAATCGG 
GAGACAGCGA GGCCGAGGAG AGAGTCGACA GCCTCCCCTG CTCCACCCTA TGGCATCAGC 
GTCTTGGAGT GTGTGCGCGA CTCCATGGTG CTGGGCTGGA AACAGCCGAG CTTCATCGGC 
GGTGCTGACA TCACCGGCTA CTTCGTGGAT TACCGCGAGG TCATCGATGG CGTGCCGGAG 
AAGTGGCACG AGGCCAACAT CAAGGCTGTC AGTGAGAGGG CCTACAGAGT GTCCGACCTG 
ATGGAGAACA GGAAGTACCA GTTCCAGGTG CGAGCAGCCA ACATGGCAGG TGTTGGGATC 
CCGTCGCTGG CCAGTGACAC CTTCCTGTGT GAGGAGTGGA CCATCGCTGT GCCAGGACCT 
CCTCATGACC TGCAGGTGAG AGAGGTGCGC AACGACTCTC TGGTATTGCT ATGGAAACCA 
CCTGTGTACC AGGGCCGCGA TCCGGTCAAC GGGTTCTACA TTGACATCAA GGAAGCGGAC 
GCCCCAGAGG AGGCGTGGAG AGGCGTCAAC AACAAGGCCA GGGAGAAGAC GTACATGAAG 
ATTACGAACC TGAAGGAGGG AGAGGTGTAC ATCTTCCGTG TTCGTGCTCA GAATAAAGCC 
GGTGTTGGAA AAACATCTGA CGTGACAGAA CCGGTTTCAG CTGTGACCAA ACCTGGCACT 
AAGGAGATCG TCGTGGACGT AGACGATGAT GGCATCATCT CCCTGAACTT TGAATGCTGT 
GACCTGACCC CTGACTCCAA ATTTGTGTGG TCCAAGAATT ACGAGGAGAT CACAGACCCC 
TCACGCTTGA CTATGGAAAC CAAGGGAAAC AAATCGAAAG CTGTTTTCAA CACTCCTGGA 
GAGGAGGACA TCGGCATTTA CTCCTGTATC GTCACACACA CTGATGGAGC TTCATCCAGC 
TACGATCTCT CTGAGGAAGA GTTGAAGAGG CTGCTGGAGG TCAGTCACGA CCACAAATTC 
CCCATTATTC CCCTGAAGTC ACAGTTGGCT GTGGAACTGC TGGAGAAAGG CAGAGTTCGC 
TTTTGGCTGC AGGCCGAGAA GATTTCTGCT GATGGCAAAG TCGATTACGT GTTTAATGAA 
AATCTCATCT CTCAGGGGGA GAAATATAAG ATGAACTTCG ACAAGAGCAC CGGTGTGATC 
GAGATGATCA TGGAGTCTCT GACCCCAGCG GACGAGGGCA CCTTCACCTT CCAGCTGAAA 
GACGGGAAAG CAACCAACCA GTCCAGCTTG GTGCTGATAG GAAACGTGTT CAAGGAGCTT 
CAGAAGGAAT CAGAGTTCCA GAGGAAAGAA TGGTTCAGAA AGCAAGGTCC TCACTTCATC 
GAGTATTTGA GTTATGAGGT GACGCCCGAG TGCTGCGTTG TGCTGAAATG CAAGGTCGGC 
AACGTGAAGA AGGAAACCTC GGCGCTGTGG TACAAAGACG GACGAGAGAT CAAGGCAGAA 
GAGCATCTGA GCTTCACAGA GGGAGTGTTG AAACTGGAAA TAGCTCAGAT TTCCAAGAGG 
GATTCTGGTG TGTACGAGGT CGTTCTGAAG GACGACAGAG GAAAAGACAC GTCCACATTG 
AATTTAACAG ATCAAGGTTT CAAGGACTTG ATGAATGAAG TTTTCAGTTT TATTGCCAAT 
TCCTCCACTC CACTGAAGAT CACGAGCACA GATGAGGGCA TCCGACTCTA CACCTTCGTC 
AACTTCTATA ACGATTTACT CCAAGTGACA TGGCACTACA AAGAATCAGC CATCGCCTTC 
TCTGACCGTA TAAAGAGCGG CGTGGTGGGG GAGCAGCTGT GGCTGCAGAT CACTGAGCCC 
ACAGAGAAAG ACACGGGCAA GTACGCCATC GAGTTCCACG ACGGAAAGGG AGGCCTGAGG 
AGAACTGTAG AGTTGTCTGG TCAAGCATTT GATGACGCTT TTGCAGAATT CCAGAGACTC 
AAAGCCGCTG CTGTTGCAGA GAGAGGTCGT GCTCGAGTAG CAGGAGGTTT GCCTGATGTG 
GTCACCATCC AGGAGGGCAA GGCCCTCAAT CTCACCTGCA ACATTTCGGG AGACCCCGTG 
CCAAAGGTCA CCTGGCTGAA GAACGACAGG GAAATCACGT CCGACGACCA CTGCATCCTG 
AAGTTCGAGT CCGGCAAGTT CGCCAGTTTC ACCATCACGG CTGTGAACAC GTCGGACACC 
GGAAAATACA GCATCCTGGT GAAGAACAAG TACGGCACAG AGAGCGGGGA CTTCACCCTG 
GAGACCACGG AGGGGACGCC TGACCTGGGC GTGACCTCAT ACTGGACCGA CGCAGATGGA 
AATGTGGTGT TTGGCCCAAA CACTCCCAAG GAAAAACGAA AGGAGTCTAT GGCTAAAGCT 
GCGAAAATGA AGGAAGACAA AAAAGAGAGA AAAAGCGAGG GATCGGAGGA GCAGAAAAAT 
GAGATTAAAG TTGATGCCGC TGCAGAAACA AAAGCTTCTG ATTCAAAACA AACAGGAGAC 
GTAAAGAAAG ATTCAGCCGA CCAATCAGAA ACCAGTGAGC GGCCTGCAGA CACAACGGAG 
GAGCCGGAAC CAGAGGCTGA GACGACACAG CCGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myom1 ( XM_020087595.1 ) cDNA ORF clone, Paralichthys olivaceus(Japanese flounder) -> XP_019943154.1 Paralichthys olivaceus myomesin 1 (myom1), transcript variant X1, mRNA.

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CloneID	OPa240836
Clone ID Related Accession (Same CDS sequence)	XM_020087595.1
Accession Version	XM_020087595.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5190bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-07
Organism	Paralichthys olivaceus(Japanese flounder)
Product	myomesin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017859653.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Paralichthys olivaceus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAT CTTTACCGTT CTACCAGAAG CACCACCGTC ACTACGACCG TGGCTACCGT 
AACAAGGAGA TGGCATCTAA AATGAGCCAG TACCAGTCCA GCAGCAGCAG CAGCAGCAGA 
TACTTTGCGG GCAGCAGCAC CTCCGCCACC AGCAGCAGCA GCAGCAGAGG ACTTAAAGTG 
TCCTCACACT CTGGGCTGGA AGAGAGCAGA CTGAGCCCCA TACCCAAGAG AACCAAGCCA 
ACTTACTTGG CTGTGGACAA AGAAAACCAA ATCATCGGCT ATGTAGTGCC TATCTTCAGG 
GGCAGTCAAG AGTTTGCTAC AGGATTTTCG GATACAGGCG AATCAAGGGT GAGGGACATG 
GCCCGCAGGG ATTTGTTCAG CAGCGGCCTG GAGATGGAGA GGTCAGAGCA GATCTCCAGG 
AAGCAGGCCA TGCGCGAGTC AGCTGATCGC ATCTCTCTGA GCAAAAGGAT CCATGAACAC 
GAGGAACACT TCAAGCGTAT GAACGAAGAC AGCCTGATGC ACCCCCCGGA GTTTGTGATT 
AAACCTCGCT CCCACACTGT GTGGGAGAAG CAGTGTGTGC GGCTGCACTG CACTGTCAGT 
GGCTGGCCTG ACCCACGGGT CGTCTGGTAC AAAAACAATG TGGCCATTGA CCCCATGGCA 
AACCCTGGCA AGTATAAGCT TGAGAGCAGA TACAGCGTCC ATTCACTGGA TATTAACAGA 
TGCGATTTTG ATGACACAGC TCAGTATCGC GTCTCCGCCA TGAACTCCAA GGGAGAGCTC 
TCTGCATTCG CCTCCATAGT TGTTAAAAGG TTCAAAGGTG AAGTTGATGA GTTCCTGCCA 
GCTCCCAGAC GGGCCATTAC TAAAGATGGC CCAGTGTCTG AGTATGGCAT CACCTTCCAG 
ACTCACATTG TCGATAAGTT TGGCGTGTCA TTTGGAAGAG AGGGGGAGAC CATGAGTCTG 
GGGTGCACAG TCGTCATCTA CCCGTCCCTG CACCTCTACC AGCCAGAGGT CCAGTGGTAC 
AAAGATGATG TCCTGCTGTC TCCATCTAAA TGGTACCAAA TGCGCTGGAG CGGAGACAGA 
GCCACACTGA CGCTCACACA CCTGAACAAG GAGGACGAGG GTCTCTACAC CCTGAGAGTC 
ACCACCAAGT CTGGATATGA AACCTACTCC TCCTATGTCT TCGTCAGAGA TTCTGATGCT 
GAGGTGGAAG GAGCTCCAGG AGCCCCTCTG GATGTTCGCT GTCTCGATGC CAACAAGGAC 
TACATCATTG TCACTTGGAA GCAGCCGGCT GTCGATGGTG GCAACTCCAT CATGGGCTAC 
TTTGTGGACA GGTGTGAGGT TGGAACCAAC CACTGGATCC AGTGCAATGA CACTCCAGTC 
AAGTTCGCCC GTTTCCCCGT GACCGGTTTG GTGGAGGGTC GTTCTTATTT CTTCCGCGTG 
CGTGCGGTCA ACAACAGCGG CATGAGTCAT CCGTCCAGAG TGTCTGAACC CGTGGCCGCC 
ATGGACCCGG CTGACCGCGC CCGCTTGAGA GGTACTTCTG CTCCCTGGAC TGGCCAAATC 
ATTGTCACAG AGGAGGAACC TGCAGAGGGC ATTGTTCCTG GCAGACCTCT TGAACTCCAG 
GTGACGGAGG CCACCAAGAA TTACGTAGTG CTGAGCTGGA AGCCACCTGG AGAGAAAGGC 
CTGGAGGGAG TGATGTACTA CGTGGAGAAG TGTGTCTCGG GCACAGACAG CTGGCAGAGG 
GTGAACACAG AGATCCCAGT CAAATCCCCT CGCTTTGCGT TGTTCGATCT GGCCGAGGGG 
AAGTCCTACA GCTTCCGTGT CCGGTGCTGC AACCCTGCCG GGGTCGGTGA ACCCTCTGAC 
CCCACTGAGG CCACCACAGT TGGCGACAAG CTTGATATCC CATCAGCCCC CAGCAAAGTC 
GTCCCCACTA GAAACACAGA CACGTCAGTC GTCGTGTCTT GGGCAGCGTC CAGTGATGCC 
AAAGAGTTGG TTGGTTACTA CATTGAGGGG AGCATCGTGG GCAGCAACGT GTGGGAGCCG 
TGCAACAACA AGCCTGTAAA TGTAACCAGG TTCATCTGCC ACGGTCTGAT GACGGGAGAG 
AAGTACGTGT TCAGGGTGAG GGCAGTGAAC GCAGCAGGAC TCAGCCAGTT CTCCCCAGAG 
TCTGAGCCTG TTGAGGTGAA GGCTGCTATT GGGGGCGGCA TCCCTCATGG TGTGTTGCCG 
GAGATGGGGC CGGGGGGTAA CGTTGGCCAA CTAACTGAAC ACAGGCCACA CTGGACGGGC 
ACGCATGAAA CTGTCCAGCC CTCCCCTGAC ACTAAGCAAA AGTGCAATAA AAAAGCTCAG 
ACTGACACTG GAGCCAGGGA GGGGGCCGGG GCTGCTGGGT GCGGAGGCCG CACAGAGCCC 
GACCCCTCCG AGAGTAAAAA CAAGGGGTGC AAGAGAGGCA GTGTGTCCTG GGCCCCTGAC 
CTGGCAAAAG ACCTGGTCTC AGAGTCCGTG TCAGGCTCGG TTTCTAACTC ACACACTGAC 
AAAGACACAG CCACATGCTT AGAGCCAGTC ACTGCTCAGG TTCAGGATGT AGAGACAGCT 
GACGGGCCTG AGGCCGCGCA GGCACGTAAG TTCTCTGTAG ACCCTAATCG GGAGACAGCG 
AGGCCGAGGA GAGAGTCGAC AGCCTCCCCT GCTCCACCCT ATGGCATCAG CGTCTTGGAG 
TGTGTGCGCG ACTCCATGGT GCTGGGCTGG AAACAGCCGA GCTTCATCGG CGGTGCTGAC 
ATCACCGGCT ACTTCGTGGA TTACCGCGAG GTCATCGATG GCGTGCCGGA GAAGTGGCAC 
GAGGCCAACA TCAAGGCTGT CAGTGAGAGG GCCTACAGAG TGTCCGACCT GATGGAGAAC 
AGGAAGTACC AGTTCCAGGT GCGAGCAGCC AACATGGCAG GTGTTGGGAT CCCGTCGCTG 
GCCAGTGACA CCTTCCTGTG TGAGGAGTGG ACCATCGCTG TGCCAGGACC TCCTCATGAC 
CTGCAGGTGA GAGAGGTGCG CAACGACTCT CTGGTATTGC TATGGAAACC ACCTGTGTAC 
CAGGGCCGCG ATCCGGTCAA CGGGTTCTAC ATTGACATCA AGGAAGCGGA CGCCCCAGAG 
GAGGCGTGGA GAGGCGTCAA CAACAAGGCC AGGGAGAAGA CGTACATGAA GATTACGAAC 
CTGAAGGAGG GAGAGGTGTA CATCTTCCGT GTTCGTGCTC AGAATAAAGC CGGTGTTGGA 
AAAACATCTG ACGTGACAGA ACCGGTTTCA GCTGTGACCA AACCTGGCAC TAAGGAGATC 
GTCGTGGACG TAGACGATGA TGGCATCATC TCCCTGAACT TTGAATGCTG TGACCTGACC 
CCTGACTCCA AATTTGTGTG GTCCAAGAAT TACGAGGAGA TCACAGACCC CTCACGCTTG 
ACTATGGAAA CCAAGGGAAA CAAATCGAAA GCTGTTTTCA ACACTCCTGG AGAGGAGGAC 
ATCGGCATTT ACTCCTGTAT CGTCACACAC ACTGATGGAG CTTCATCCAG CTACGATCTC 
TCTGAGGAAG AGTTGAAGAG GCTGCTGGAG GTCAGTCACG ACCACAAATT CCCCATTATT 
CCCCTGAAGT CACAGTTGGC TGTGGAACTG CTGGAGAAAG GCAGAGTTCG CTTTTGGCTG 
CAGGCCGAGA AGATTTCTGC TGATGGCAAA GTCGATTACG TGTTTAATGA AAATCTCATC 
TCTCAGGGGG AGAAATATAA GATGAACTTC GACAAGAGCA CCGGTGTGAT CGAGATGATC 
ATGGAGTCTC TGACCCCAGC GGACGAGGGC ACCTTCACCT TCCAGCTGAA AGACGGGAAA 
GCAACCAACC AGTCCAGCTT GGTGCTGATA GGAAACGTGT TCAAGGAGCT TCAGAAGGAA 
TCAGAGTTCC AGAGGAAAGA ATGGTTCAGA AAGCAAGGTC CTCACTTCAT CGAGTATTTG 
AGTTATGAGG TGACGCCCGA GTGCTGCGTT GTGCTGAAAT GCAAGGTCGG CAACGTGAAG 
AAGGAAACCT CGGCGCTGTG GTACAAAGAC GGACGAGAGA TCAAGGCAGA AGAGCATCTG 
AGCTTCACAG AGGGAGTGTT GAAACTGGAA ATAGCTCAGA TTTCCAAGAG GGATTCTGGT 
GTGTACGAGG TCGTTCTGAA GGACGACAGA GGAAAAGACA CGTCCACATT GAATTTAACA 
GATCAAGGTT TCAAGGACTT GATGAATGAA GTTTTCAGTT TTATTGCCAA TTCCTCCACT 
CCACTGAAGA TCACGAGCAC AGATGAGGGC ATCCGACTCT ACACCTTCGT CAACTTCTAT 
AACGATTTAC TCCAAGTGAC ATGGCACTAC AAAGAATCAG CCATCGCCTT CTCTGACCGT 
ATAAAGAGCG GCGTGGTGGG GGAGCAGCTG TGGCTGCAGA TCACTGAGCC CACAGAGAAA 
GACACGGGCA AGTACGCCAT CGAGTTCCAC GACGGAAAGG GAGGCCTGAG GAGAACTGTA 
GAGTTGTCTG GTCAAGCATT TGATGACGCT TTTGCAGAAT TCCAGAGACT CAAAGCCGCT 
GCTGTTGCAG AGAGAGGTCG TGCTCGAGTA GCAGGAGGTT TGCCTGATGT GGTCACCATC 
CAGGAGGGCA AGGCCCTCAA TCTCACCTGC AACATTTCGG GAGACCCCGT GCCAAAGGTC 
ACCTGGCTGA AGAACGACAG GGAAATCACG TCCGACGACC ACTGCATCCT GAAGTTCGAG 
TCCGGCAAGT TCGCCAGTTT CACCATCACG GCTGTGAACA CGTCGGACAC CGGAAAATAC 
AGCATCCTGG TGAAGAACAA GTACGGCACA GAGAGCGGGG ACTTCACCCT GGAGACCACG 
GAGGGGACGC CTGACCTGGG CGTGACCTCA TACTGGACCG ACGCAGATGG AAATGTGGTG 
TTTGGCCCAA ACACTCCCAA GGAAAAACGA AAGGAGTCTA TGGCTAAAGC TGCGAAAATG 
AAGGAAGACA AAAAAGAGAG AAAAAGCGAG GGATCGGAGG AGCAGAAAAA TGAGATTAAA 
GTTGATGCCG CTGCAGAAAC AAAAGCTTCT GATTCAAAAC AAACAGGAGA CGTAAAGAAA 
GATTCAGCCG ACCAATCAGA AACCAGTGAG CGGCCTGCAG ACACAACGGA GGAGCCGGAA 
CCAGAGGCTG AGACGACACA GCCGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019943154.1
CDS	179..5368
Translation	MSGSLPFYQKHHRHYDRGYRNKEMASKMSQYQSSSSSSSRYFAGSSTSATSSSSSRGLKVSSHSGLEESRLSPIPKRTKPTYLAVDKENQIIGYVVPIFRGSQEFATGFSDTGESRVRDMARRDLFSSGLEMERSEQISRKQAMRESADRISLSKRIHEHEEHFKRMNEDSLMHPPEFVIKPRSHTVWEKQCVRLHCTVSGWPDPRVVWYKNNVAIDPMANPGKYKLESRYSVHSLDINRCDFDDTAQYRVSAMNSKGELSAFASIVVKRFKGEVDEFLPAPRRAITKDGPVSEYGITFQTHIVDKFGVSFGREGETMSLGCTVVIYPSLHLYQPEVQWYKDDVLLSPSKWYQMRWSGDRATLTLTHLNKEDEGLYTLRVTTKSGYETYSSYVFVRDSDAEVEGAPGAPLDVRCLDANKDYIIVTWKQPAVDGGNSIMGYFVDRCEVGTNHWIQCNDTPVKFARFPVTGLVEGRSYFFRVRAVNNSGMSHPSRVSEPVAAMDPADRARLRGTSAPWTGQIIVTEEEPAEGIVPGRPLELQVTEATKNYVVLSWKPPGEKGLEGVMYYVEKCVSGTDSWQRVNTEIPVKSPRFALFDLAEGKSYSFRVRCCNPAGVGEPSDPTEATTVGDKLDIPSAPSKVVPTRNTDTSVVVSWAASSDAKELVGYYIEGSIVGSNVWEPCNNKPVNVTRFICHGLMTGEKYVFRVRAVNAAGLSQFSPESEPVEVKAAIGGGIPHGVLPEMGPGGNVGQLTEHRPHWTGTHETVQPSPDTKQKCNKKAQTDTGAREGAGAAGCGGRTEPDPSESKNKGCKRGSVSWAPDLAKDLVSESVSGSVSNSHTDKDTATCLEPVTAQVQDVETADGPEAAQARKFSVDPNRETARPRRESTASPAPPYGISVLECVRDSMVLGWKQPSFIGGADITGYFVDYREVIDGVPEKWHEANIKAVSERAYRVSDLMENRKYQFQVRAANMAGVGIPSLASDTFLCEEWTIAVPGPPHDLQVREVRNDSLVLLWKPPVYQGRDPVNGFYIDIKEADAPEEAWRGVNNKAREKTYMKITNLKEGEVYIFRVRAQNKAGVGKTSDVTEPVSAVTKPGTKEIVVDVDDDGIISLNFECCDLTPDSKFVWSKNYEEITDPSRLTMETKGNKSKAVFNTPGEEDIGIYSCIVTHTDGASSSYDLSEEELKRLLEVSHDHKFPIIPLKSQLAVELLEKGRVRFWLQAEKISADGKVDYVFNENLISQGEKYKMNFDKSTGVIEMIMESLTPADEGTFTFQLKDGKATNQSSLVLIGNVFKELQKESEFQRKEWFRKQGPHFIEYLSYEVTPECCVVLKCKVGNVKKETSALWYKDGREIKAEEHLSFTEGVLKLEIAQISKRDSGVYEVVLKDDRGKDTSTLNLTDQGFKDLMNEVFSFIANSSTPLKITSTDEGIRLYTFVNFYNDLLQVTWHYKESAIAFSDRIKSGVVGEQLWLQITEPTEKDTGKYAIEFHDGKGGLRRTVELSGQAFDDAFAEFQRLKAAAVAERGRARVAGGLPDVVTIQEGKALNLTCNISGDPVPKVTWLKNDREITSDDHCILKFESGKFASFTITAVNTSDTGKYSILVKNKYGTESGDFTLETTEGTPDLGVTSYWTDADGNVVFGPNTPKEKRKESMAKAAKMKEDKKERKSEGSEEQKNEIKVDAAAETKASDSKQTGDVKKDSADQSETSERPADTTEEPEPEAETTQPK

Target ORF information:

RefSeq Version	XM_020087595.1
Organism	Paralichthys olivaceus(Japanese flounder)
Definition	Paralichthys olivaceus myomesin 1 (myom1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020087595.1

ORF Insert Sequence:

ATGTCTGGAT CTTTACCGTT CTACCAGAAG CACCACCGTC ACTACGACCG TGGCTACCGT 
AACAAGGAGA TGGCATCTAA AATGAGCCAG TACCAGTCCA GCAGCAGCAG CAGCAGCAGA 
TACTTTGCGG GCAGCAGCAC CTCCGCCACC AGCAGCAGCA GCAGCAGAGG ACTTAAAGTG 
TCCTCACACT CTGGGCTGGA AGAGAGCAGA CTGAGCCCCA TACCCAAGAG AACCAAGCCA 
ACTTACTTGG CTGTGGACAA AGAAAACCAA ATCATCGGCT ATGTAGTGCC TATCTTCAGG 
GGCAGTCAAG AGTTTGCTAC AGGATTTTCG GATACAGGCG AATCAAGGGT GAGGGACATG 
GCCCGCAGGG ATTTGTTCAG CAGCGGCCTG GAGATGGAGA GGTCAGAGCA GATCTCCAGG 
AAGCAGGCCA TGCGCGAGTC AGCTGATCGC ATCTCTCTGA GCAAAAGGAT CCATGAACAC 
GAGGAACACT TCAAGCGTAT GAACGAAGAC AGCCTGATGC ACCCCCCGGA GTTTGTGATT 
AAACCTCGCT CCCACACTGT GTGGGAGAAG CAGTGTGTGC GGCTGCACTG CACTGTCAGT 
GGCTGGCCTG ACCCACGGGT CGTCTGGTAC AAAAACAATG TGGCCATTGA CCCCATGGCA 
AACCCTGGCA AGTATAAGCT TGAGAGCAGA TACAGCGTCC ATTCACTGGA TATTAACAGA 
TGCGATTTTG ATGACACAGC TCAGTATCGC GTCTCCGCCA TGAACTCCAA GGGAGAGCTC 
TCTGCATTCG CCTCCATAGT TGTTAAAAGG TTCAAAGGTG AAGTTGATGA GTTCCTGCCA 
GCTCCCAGAC GGGCCATTAC TAAAGATGGC CCAGTGTCTG AGTATGGCAT CACCTTCCAG 
ACTCACATTG TCGATAAGTT TGGCGTGTCA TTTGGAAGAG AGGGGGAGAC CATGAGTCTG 
GGGTGCACAG TCGTCATCTA CCCGTCCCTG CACCTCTACC AGCCAGAGGT CCAGTGGTAC 
AAAGATGATG TCCTGCTGTC TCCATCTAAA TGGTACCAAA TGCGCTGGAG CGGAGACAGA 
GCCACACTGA CGCTCACACA CCTGAACAAG GAGGACGAGG GTCTCTACAC CCTGAGAGTC 
ACCACCAAGT CTGGATATGA AACCTACTCC TCCTATGTCT TCGTCAGAGA TTCTGATGCT 
GAGGTGGAAG GAGCTCCAGG AGCCCCTCTG GATGTTCGCT GTCTCGATGC CAACAAGGAC 
TACATCATTG TCACTTGGAA GCAGCCGGCT GTCGATGGTG GCAACTCCAT CATGGGCTAC 
TTTGTGGACA GGTGTGAGGT TGGAACCAAC CACTGGATCC AGTGCAATGA CACTCCAGTC 
AAGTTCGCCC GTTTCCCCGT GACCGGTTTG GTGGAGGGTC GTTCTTATTT CTTCCGCGTG 
CGTGCGGTCA ACAACAGCGG CATGAGTCAT CCGTCCAGAG TGTCTGAACC CGTGGCCGCC 
ATGGACCCGG CTGACCGCGC CCGCTTGAGA GGTACTTCTG CTCCCTGGAC TGGCCAAATC 
ATTGTCACAG AGGAGGAACC TGCAGAGGGC ATTGTTCCTG GCAGACCTCT TGAACTCCAG 
GTGACGGAGG CCACCAAGAA TTACGTAGTG CTGAGCTGGA AGCCACCTGG AGAGAAAGGC 
CTGGAGGGAG TGATGTACTA CGTGGAGAAG TGTGTCTCGG GCACAGACAG CTGGCAGAGG 
GTGAACACAG AGATCCCAGT CAAATCCCCT CGCTTTGCGT TGTTCGATCT GGCCGAGGGG 
AAGTCCTACA GCTTCCGTGT CCGGTGCTGC AACCCTGCCG GGGTCGGTGA ACCCTCTGAC 
CCCACTGAGG CCACCACAGT TGGCGACAAG CTTGATATCC CATCAGCCCC CAGCAAAGTC 
GTCCCCACTA GAAACACAGA CACGTCAGTC GTCGTGTCTT GGGCAGCGTC CAGTGATGCC 
AAAGAGTTGG TTGGTTACTA CATTGAGGGG AGCATCGTGG GCAGCAACGT GTGGGAGCCG 
TGCAACAACA AGCCTGTAAA TGTAACCAGG TTCATCTGCC ACGGTCTGAT GACGGGAGAG 
AAGTACGTGT TCAGGGTGAG GGCAGTGAAC GCAGCAGGAC TCAGCCAGTT CTCCCCAGAG 
TCTGAGCCTG TTGAGGTGAA GGCTGCTATT GGGGGCGGCA TCCCTCATGG TGTGTTGCCG 
GAGATGGGGC CGGGGGGTAA CGTTGGCCAA CTAACTGAAC ACAGGCCACA CTGGACGGGC 
ACGCATGAAA CTGTCCAGCC CTCCCCTGAC ACTAAGCAAA AGTGCAATAA AAAAGCTCAG 
ACTGACACTG GAGCCAGGGA GGGGGCCGGG GCTGCTGGGT GCGGAGGCCG CACAGAGCCC 
GACCCCTCCG AGAGTAAAAA CAAGGGGTGC AAGAGAGGCA GTGTGTCCTG GGCCCCTGAC 
CTGGCAAAAG ACCTGGTCTC AGAGTCCGTG TCAGGCTCGG TTTCTAACTC ACACACTGAC 
AAAGACACAG CCACATGCTT AGAGCCAGTC ACTGCTCAGG TTCAGGATGT AGAGACAGCT 
GACGGGCCTG AGGCCGCGCA GGCACGTAAG TTCTCTGTAG ACCCTAATCG GGAGACAGCG 
AGGCCGAGGA GAGAGTCGAC AGCCTCCCCT GCTCCACCCT ATGGCATCAG CGTCTTGGAG 
TGTGTGCGCG ACTCCATGGT GCTGGGCTGG AAACAGCCGA GCTTCATCGG CGGTGCTGAC 
ATCACCGGCT ACTTCGTGGA TTACCGCGAG GTCATCGATG GCGTGCCGGA GAAGTGGCAC 
GAGGCCAACA TCAAGGCTGT CAGTGAGAGG GCCTACAGAG TGTCCGACCT GATGGAGAAC 
AGGAAGTACC AGTTCCAGGT GCGAGCAGCC AACATGGCAG GTGTTGGGAT CCCGTCGCTG 
GCCAGTGACA CCTTCCTGTG TGAGGAGTGG ACCATCGCTG TGCCAGGACC TCCTCATGAC 
CTGCAGGTGA GAGAGGTGCG CAACGACTCT CTGGTATTGC TATGGAAACC ACCTGTGTAC 
CAGGGCCGCG ATCCGGTCAA CGGGTTCTAC ATTGACATCA AGGAAGCGGA CGCCCCAGAG 
GAGGCGTGGA GAGGCGTCAA CAACAAGGCC AGGGAGAAGA CGTACATGAA GATTACGAAC 
CTGAAGGAGG GAGAGGTGTA CATCTTCCGT GTTCGTGCTC AGAATAAAGC CGGTGTTGGA 
AAAACATCTG ACGTGACAGA ACCGGTTTCA GCTGTGACCA AACCTGGCAC TAAGGAGATC 
GTCGTGGACG TAGACGATGA TGGCATCATC TCCCTGAACT TTGAATGCTG TGACCTGACC 
CCTGACTCCA AATTTGTGTG GTCCAAGAAT TACGAGGAGA TCACAGACCC CTCACGCTTG 
ACTATGGAAA CCAAGGGAAA CAAATCGAAA GCTGTTTTCA ACACTCCTGG AGAGGAGGAC 
ATCGGCATTT ACTCCTGTAT CGTCACACAC ACTGATGGAG CTTCATCCAG CTACGATCTC 
TCTGAGGAAG AGTTGAAGAG GCTGCTGGAG GTCAGTCACG ACCACAAATT CCCCATTATT 
CCCCTGAAGT CACAGTTGGC TGTGGAACTG CTGGAGAAAG GCAGAGTTCG CTTTTGGCTG 
CAGGCCGAGA AGATTTCTGC TGATGGCAAA GTCGATTACG TGTTTAATGA AAATCTCATC 
TCTCAGGGGG AGAAATATAA GATGAACTTC GACAAGAGCA CCGGTGTGAT CGAGATGATC 
ATGGAGTCTC TGACCCCAGC GGACGAGGGC ACCTTCACCT TCCAGCTGAA AGACGGGAAA 
GCAACCAACC AGTCCAGCTT GGTGCTGATA GGAAACGTGT TCAAGGAGCT TCAGAAGGAA 
TCAGAGTTCC AGAGGAAAGA ATGGTTCAGA AAGCAAGGTC CTCACTTCAT CGAGTATTTG 
AGTTATGAGG TGACGCCCGA GTGCTGCGTT GTGCTGAAAT GCAAGGTCGG CAACGTGAAG 
AAGGAAACCT CGGCGCTGTG GTACAAAGAC GGACGAGAGA TCAAGGCAGA AGAGCATCTG 
AGCTTCACAG AGGGAGTGTT GAAACTGGAA ATAGCTCAGA TTTCCAAGAG GGATTCTGGT 
GTGTACGAGG TCGTTCTGAA GGACGACAGA GGAAAAGACA CGTCCACATT GAATTTAACA 
GATCAAGGTT TCAAGGACTT GATGAATGAA GTTTTCAGTT TTATTGCCAA TTCCTCCACT 
CCACTGAAGA TCACGAGCAC AGATGAGGGC ATCCGACTCT ACACCTTCGT CAACTTCTAT 
AACGATTTAC TCCAAGTGAC ATGGCACTAC AAAGAATCAG CCATCGCCTT CTCTGACCGT 
ATAAAGAGCG GCGTGGTGGG GGAGCAGCTG TGGCTGCAGA TCACTGAGCC CACAGAGAAA 
GACACGGGCA AGTACGCCAT CGAGTTCCAC GACGGAAAGG GAGGCCTGAG GAGAACTGTA 
GAGTTGTCTG GTCAAGCATT TGATGACGCT TTTGCAGAAT TCCAGAGACT CAAAGCCGCT 
GCTGTTGCAG AGAGAGGTCG TGCTCGAGTA GCAGGAGGTT TGCCTGATGT GGTCACCATC 
CAGGAGGGCA AGGCCCTCAA TCTCACCTGC AACATTTCGG GAGACCCCGT GCCAAAGGTC 
ACCTGGCTGA AGAACGACAG GGAAATCACG TCCGACGACC ACTGCATCCT GAAGTTCGAG 
TCCGGCAAGT TCGCCAGTTT CACCATCACG GCTGTGAACA CGTCGGACAC CGGAAAATAC 
AGCATCCTGG TGAAGAACAA GTACGGCACA GAGAGCGGGG ACTTCACCCT GGAGACCACG 
GAGGGGACGC CTGACCTGGG CGTGACCTCA TACTGGACCG ACGCAGATGG AAATGTGGTG 
TTTGGCCCAA ACACTCCCAA GGAAAAACGA AAGGAGTCTA TGGCTAAAGC TGCGAAAATG 
AAGGAAGACA AAAAAGAGAG AAAAAGCGAG GGATCGGAGG AGCAGAAAAA TGAGATTAAA 
GTTGATGCCG CTGCAGAAAC AAAAGCTTCT GATTCAAAAC AAACAGGAGA CGTAAAGAAA 
GATTCAGCCG ACCAATCAGA AACCAGTGAG CGGCCTGCAG ACACAACGGA GGAGCCGGAA 
CCAGAGGCTG AGACGACACA GCCGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myom1 ( XM_020087596.1 ) cDNA ORF clone, Paralichthys olivaceus(Japanese flounder) -> XP_019943155.1 Paralichthys olivaceus myomesin 1 (myom1), transcript variant X2, mRNA.

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CloneID	OPa240837
Clone ID Related Accession (Same CDS sequence)	XM_020087596.1
Accession Version	XM_020087596.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4719bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-07
Organism	Paralichthys olivaceus(Japanese flounder)
Product	myomesin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017859653.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Paralichthys olivaceus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAT CTTTACCGTT CTACCAGAAG CACCACCGTC ACTACGACCG TGGCTACCGT 
AACAAGGAGA TGGCATCTAA AATGAGCCAG TACCAGTCCA GCAGCAGCAG CAGCAGCAGA 
TACTTTGCGG GCAGCAGCAC CTCCGCCACC AGCAGCAGCA GCAGCAGAGG ACTTAAAGTG 
TCCTCACACT CTGGGCTGGA AGAGAGCAGA CTGAGCCCCA TACCCAAGAG AACCAAGCCA 
ACTTACTTGG CTGTGGACAA AGAAAACCAA ATCATCGGCT ATGTAGTGCC TATCTTCAGG 
GGCAGTCAAG AGTTTGCTAC AGGATTTTCG GATACAGGCG AATCAAGGGT GAGGGACATG 
GCCCGCAGGG ATTTGTTCAG CAGCGGCCTG GAGATGGAGA GGTCAGAGCA GATCTCCAGG 
AAGCAGGCCA TGCGCGAGTC AGCTGATCGC ATCTCTCTGA GCAAAAGGAT CCATGAACAC 
GAGGAACACT TCAAGCGTAT GAACGAAGAC AGCCTGATGC ACCCCCCGGA GTTTGTGATT 
AAACCTCGCT CCCACACTGT GTGGGAGAAG CAGTGTGTGC GGCTGCACTG CACTGTCAGT 
GGCTGGCCTG ACCCACGGGT CGTCTGGTAC AAAAACAATG TGGCCATTGA CCCCATGGCA 
AACCCTGGCA AGTATAAGCT TGAGAGCAGA TACAGCGTCC ATTCACTGGA TATTAACAGA 
TGCGATTTTG ATGACACAGC TCAGTATCGC GTCTCCGCCA TGAACTCCAA GGGAGAGCTC 
TCTGCATTCG CCTCCATAGT TGTTAAAAGG TTCAAAGGTG AAGTTGATGA GTTCCTGCCA 
GCTCCCAGAC GGGCCATTAC TAAAGATGGC CCAGTGTCTG AGTATGGCAT CACCTTCCAG 
ACTCACATTG TCGATAAGTT TGGCGTGTCA TTTGGAAGAG AGGGGGAGAC CATGAGTCTG 
GGGTGCACAG TCGTCATCTA CCCGTCCCTG CACCTCTACC AGCCAGAGGT CCAGTGGTAC 
AAAGATGATG TCCTGCTGTC TCCATCTAAA TGGTACCAAA TGCGCTGGAG CGGAGACAGA 
GCCACACTGA CGCTCACACA CCTGAACAAG GAGGACGAGG GTCTCTACAC CCTGAGAGTC 
ACCACCAAGT CTGGATATGA AACCTACTCC TCCTATGTCT TCGTCAGAGA TTCTGATGCT 
GAGGTGGAAG GAGCTCCAGG AGCCCCTCTG GATGTTCGCT GTCTCGATGC CAACAAGGAC 
TACATCATTG TCACTTGGAA GCAGCCGGCT GTCGATGGTG GCAACTCCAT CATGGGCTAC 
TTTGTGGACA GGTGTGAGGT TGGAACCAAC CACTGGATCC AGTGCAATGA CACTCCAGTC 
AAGTTCGCCC GTTTCCCCGT GACCGGTTTG GTGGAGGGTC GTTCTTATTT CTTCCGCGTG 
CGTGCGGTCA ACAACAGCGG CATGAGTCAT CCGTCCAGAG TGTCTGAACC CGTGGCCGCC 
ATGGACCCGG CTGACCGCGC CCGCTTGAGA GGTACTTCTG CTCCCTGGAC TGGCCAAATC 
ATTGTCACAG AGGAGGAACC TGCAGAGGGC ATTGTTCCTG GCAGACCTCT TGAACTCCAG 
GTGACGGAGG CCACCAAGAA TTACGTAGTG CTGAGCTGGA AGCCACCTGG AGAGAAAGGC 
CTGGAGGGAG TGATGTACTA CGTGGAGAAG TGTGTCTCGG GCACAGACAG CTGGCAGAGG 
GTGAACACAG AGATCCCAGT CAAATCCCCT CGCTTTGCGT TGTTCGATCT GGCCGAGGGG 
AAGTCCTACA GCTTCCGTGT CCGGTGCTGC AACCCTGCCG GGGTCGGTGA ACCCTCTGAC 
CCCACTGAGG CCACCACAGT TGGCGACAAG CTTGATATCC CATCAGCCCC CAGCAAAGTC 
GTCCCCACTA GAAACACAGA CACGTCAGTC GTCGTGTCTT GGGCAGCGTC CAGTGATGCC 
AAAGAGTTGG TTGGTTACTA CATTGAGGGG AGCATCGTGG GCAGCAACGT GTGGGAGCCG 
TGCAACAACA AGCCTGTAAA TGTAACCAGG TTCATCTGCC ACGGTCTGAT GACGGGAGAG 
AAGTACGTGT TCAGGGTGAG GGCAGTGAAC GCAGCAGGAC TCAGCCAGTT CTCCCCAGAG 
TCTGAGCCTG TTGAGGTGAA GGCTGCTATT GCCTCCCCTG CTCCACCCTA TGGCATCAGC 
GTCTTGGAGT GTGTGCGCGA CTCCATGGTG CTGGGCTGGA AACAGCCGAG CTTCATCGGC 
GGTGCTGACA TCACCGGCTA CTTCGTGGAT TACCGCGAGG TCATCGATGG CGTGCCGGAG 
AAGTGGCACG AGGCCAACAT CAAGGCTGTC AGTGAGAGGG CCTACAGAGT GTCCGACCTG 
ATGGAGAACA GGAAGTACCA GTTCCAGGTG CGAGCAGCCA ACATGGCAGG TGTTGGGATC 
CCGTCGCTGG CCAGTGACAC CTTCCTGTGT GAGGAGTGGA CCATCGCTGT GCCAGGACCT 
CCTCATGACC TGCAGGTGAG AGAGGTGCGC AACGACTCTC TGGTATTGCT ATGGAAACCA 
CCTGTGTACC AGGGCCGCGA TCCGGTCAAC GGGTTCTACA TTGACATCAA GGAAGCGGAC 
GCCCCAGAGG AGGCGTGGAG AGGCGTCAAC AACAAGGCCA GGGAGAAGAC GTACATGAAG 
ATTACGAACC TGAAGGAGGG AGAGGTGTAC ATCTTCCGTG TTCGTGCTCA GAATAAAGCC 
GGTGTTGGAA AAACATCTGA CGTGACAGAA CCGGTTTCAG CTGTGACCAA ACCTGGCACT 
AAGGAGATCG TCGTGGACGT AGACGATGAT GGCATCATCT CCCTGAACTT TGAATGCTGT 
GACCTGACCC CTGACTCCAA ATTTGTGTGG TCCAAGAATT ACGAGGAGAT CACAGACCCC 
TCACGCTTGA CTATGGAAAC CAAGGGAAAC AAATCGAAAG CTGTTTTCAA CACTCCTGGA 
GAGGAGGACA TCGGCATTTA CTCCTGTATC GTCACACACA CTGATGGAGC TTCATCCAGC 
TACGATCTCT CTGAGGAAGA GTTGAAGAGG CTGCTGGAGG TCAGTCACGA CCACAAATTC 
CCCATTATTC CCCTGAAGTC ACAGTTGGCT GTGGAACTGC TGGAGAAAGG CAGAGTTCGC 
TTTTGGCTGC AGGCCGAGAA GATTTCTGCT GATGGCAAAG TCGATTACGT GTTTAATGAA 
AATCTCATCT CTCAGGGGGA GAAATATAAG ATGAACTTCG ACAAGAGCAC CGGTGTGATC 
GAGATGATCA TGGAGTCTCT GACCCCAGCG GACGAGGGCA CCTTCACCTT CCAGCTGAAA 
GACGGGAAAG CAACCAACCA GTCCAGCTTG GTGCTGATAG GAAACGTGTT CAAGGAGCTT 
CAGAAGGAAT CAGAGTTCCA GAGGAAAGAA TGGTTCAGAA AGCAAGGTCC TCACTTCATC 
GAGTATTTGA GTTATGAGGT GACGCCCGAG TGCTGCGTTG TGCTGAAATG CAAGGTCGGC 
AACGTGAAGA AGGAAACCTC GGCGCTGTGG TACAAAGACG GACGAGAGAT CAAGGCAGAA 
GAGCATCTGA GCTTCACAGA GGGAGTGTTG AAACTGGAAA TAGCTCAGAT TTCCAAGAGG 
GATTCTGGTG TGTACGAGGT CGTTCTGAAG GACGACAGAG GAAAAGACAC GTCCACATTG 
AATTTAACAG ATCAAGGTTT CAAGGACTTG ATGAATGAAG TTTTCAGTTT TATTGCCAAT 
TCCTCCACTC CACTGAAGAT CACGAGCACA GATGAGGGCA TCCGACTCTA CACCTTCGTC 
AACTTCTATA ACGATTTACT CCAAGTGACA TGGCACTACA AAGAATCAGC CATCGCCTTC 
TCTGACCGTA TAAAGAGCGG CGTGGTGGGG GAGCAGCTGT GGCTGCAGAT CACTGAGCCC 
ACAGAGAAAG ACACGGGCAA GTACGCCATC GAGTTCCACG ACGGAAAGGG AGGCCTGAGG 
AGAACTGTAG AGTTGTCTGG TCAAGCATTT GATGACGCTT TTGCAGAATT CCAGAGACTC 
AAAGCCGCTG CTGTTGCAGA GAGAGGTCGT GCTCGAGTAG CAGGAGGTTT GCCTGATGTG 
GTCACCATCC AGGAGGGCAA GGCCCTCAAT CTCACCTGCA ACATTTCGGG AGACCCCGTG 
CCAAAGGTCA CCTGGCTGAA GAACGACAGG GAAATCACGT CCGACGACCA CTGCATCCTG 
AAGTTCGAGT CCGGCAAGTT CGCCAGTTTC ACCATCACGG CTGTGAACAC GTCGGACACC 
GGAAAATACA GCATCCTGGT GAAGAACAAG TACGGCACAG AGAGCGGGGA CTTCACCCTG 
GAGACCACGG AGGGGACGCC TGACCTGGGC GTGACCTCAT ACTGGACCGA CGCAGATGGA 
AATGTGGTGT TTGGCCCAAA CACTCCCAAG GAAAAACGAA AGGAGTCTAT GGCTAAAGCT 
GCGAAAATGA AGGAAGACAA AAAAGAGAGA AAAAGCGAGG GATCGGAGGA GCAGAAAAAT 
GAGATTAAAG TTGATGCCGC TGCAGAAACA AAAGCTTCTG ATTCAAAACA AACAGGAGAC 
GTAAAGAAAG ATTCAGCCGA CCAATCAGAA ACCAGTGAGC GGCCTGCAGA CACAACGGAG 
GAGCCGGAAC CAGAGGCTGA GACGACACAG CCGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019943155.1
CDS	178..4896
Translation	MSGSLPFYQKHHRHYDRGYRNKEMASKMSQYQSSSSSSSRYFAGSSTSATSSSSSRGLKVSSHSGLEESRLSPIPKRTKPTYLAVDKENQIIGYVVPIFRGSQEFATGFSDTGESRVRDMARRDLFSSGLEMERSEQISRKQAMRESADRISLSKRIHEHEEHFKRMNEDSLMHPPEFVIKPRSHTVWEKQCVRLHCTVSGWPDPRVVWYKNNVAIDPMANPGKYKLESRYSVHSLDINRCDFDDTAQYRVSAMNSKGELSAFASIVVKRFKGEVDEFLPAPRRAITKDGPVSEYGITFQTHIVDKFGVSFGREGETMSLGCTVVIYPSLHLYQPEVQWYKDDVLLSPSKWYQMRWSGDRATLTLTHLNKEDEGLYTLRVTTKSGYETYSSYVFVRDSDAEVEGAPGAPLDVRCLDANKDYIIVTWKQPAVDGGNSIMGYFVDRCEVGTNHWIQCNDTPVKFARFPVTGLVEGRSYFFRVRAVNNSGMSHPSRVSEPVAAMDPADRARLRGTSAPWTGQIIVTEEEPAEGIVPGRPLELQVTEATKNYVVLSWKPPGEKGLEGVMYYVEKCVSGTDSWQRVNTEIPVKSPRFALFDLAEGKSYSFRVRCCNPAGVGEPSDPTEATTVGDKLDIPSAPSKVVPTRNTDTSVVVSWAASSDAKELVGYYIEGSIVGSNVWEPCNNKPVNVTRFICHGLMTGEKYVFRVRAVNAAGLSQFSPESEPVEVKAAIASPAPPYGISVLECVRDSMVLGWKQPSFIGGADITGYFVDYREVIDGVPEKWHEANIKAVSERAYRVSDLMENRKYQFQVRAANMAGVGIPSLASDTFLCEEWTIAVPGPPHDLQVREVRNDSLVLLWKPPVYQGRDPVNGFYIDIKEADAPEEAWRGVNNKAREKTYMKITNLKEGEVYIFRVRAQNKAGVGKTSDVTEPVSAVTKPGTKEIVVDVDDDGIISLNFECCDLTPDSKFVWSKNYEEITDPSRLTMETKGNKSKAVFNTPGEEDIGIYSCIVTHTDGASSSYDLSEEELKRLLEVSHDHKFPIIPLKSQLAVELLEKGRVRFWLQAEKISADGKVDYVFNENLISQGEKYKMNFDKSTGVIEMIMESLTPADEGTFTFQLKDGKATNQSSLVLIGNVFKELQKESEFQRKEWFRKQGPHFIEYLSYEVTPECCVVLKCKVGNVKKETSALWYKDGREIKAEEHLSFTEGVLKLEIAQISKRDSGVYEVVLKDDRGKDTSTLNLTDQGFKDLMNEVFSFIANSSTPLKITSTDEGIRLYTFVNFYNDLLQVTWHYKESAIAFSDRIKSGVVGEQLWLQITEPTEKDTGKYAIEFHDGKGGLRRTVELSGQAFDDAFAEFQRLKAAAVAERGRARVAGGLPDVVTIQEGKALNLTCNISGDPVPKVTWLKNDREITSDDHCILKFESGKFASFTITAVNTSDTGKYSILVKNKYGTESGDFTLETTEGTPDLGVTSYWTDADGNVVFGPNTPKEKRKESMAKAAKMKEDKKERKSEGSEEQKNEIKVDAAAETKASDSKQTGDVKKDSADQSETSERPADTTEEPEPEAETTQPK

Target ORF information:

RefSeq Version	XM_020087596.1
Organism	Paralichthys olivaceus(Japanese flounder)
Definition	Paralichthys olivaceus myomesin 1 (myom1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020087596.1

ORF Insert Sequence:

ATGTCTGGAT CTTTACCGTT CTACCAGAAG CACCACCGTC ACTACGACCG TGGCTACCGT 
AACAAGGAGA TGGCATCTAA AATGAGCCAG TACCAGTCCA GCAGCAGCAG CAGCAGCAGA 
TACTTTGCGG GCAGCAGCAC CTCCGCCACC AGCAGCAGCA GCAGCAGAGG ACTTAAAGTG 
TCCTCACACT CTGGGCTGGA AGAGAGCAGA CTGAGCCCCA TACCCAAGAG AACCAAGCCA 
ACTTACTTGG CTGTGGACAA AGAAAACCAA ATCATCGGCT ATGTAGTGCC TATCTTCAGG 
GGCAGTCAAG AGTTTGCTAC AGGATTTTCG GATACAGGCG AATCAAGGGT GAGGGACATG 
GCCCGCAGGG ATTTGTTCAG CAGCGGCCTG GAGATGGAGA GGTCAGAGCA GATCTCCAGG 
AAGCAGGCCA TGCGCGAGTC AGCTGATCGC ATCTCTCTGA GCAAAAGGAT CCATGAACAC 
GAGGAACACT TCAAGCGTAT GAACGAAGAC AGCCTGATGC ACCCCCCGGA GTTTGTGATT 
AAACCTCGCT CCCACACTGT GTGGGAGAAG CAGTGTGTGC GGCTGCACTG CACTGTCAGT 
GGCTGGCCTG ACCCACGGGT CGTCTGGTAC AAAAACAATG TGGCCATTGA CCCCATGGCA 
AACCCTGGCA AGTATAAGCT TGAGAGCAGA TACAGCGTCC ATTCACTGGA TATTAACAGA 
TGCGATTTTG ATGACACAGC TCAGTATCGC GTCTCCGCCA TGAACTCCAA GGGAGAGCTC 
TCTGCATTCG CCTCCATAGT TGTTAAAAGG TTCAAAGGTG AAGTTGATGA GTTCCTGCCA 
GCTCCCAGAC GGGCCATTAC TAAAGATGGC CCAGTGTCTG AGTATGGCAT CACCTTCCAG 
ACTCACATTG TCGATAAGTT TGGCGTGTCA TTTGGAAGAG AGGGGGAGAC CATGAGTCTG 
GGGTGCACAG TCGTCATCTA CCCGTCCCTG CACCTCTACC AGCCAGAGGT CCAGTGGTAC 
AAAGATGATG TCCTGCTGTC TCCATCTAAA TGGTACCAAA TGCGCTGGAG CGGAGACAGA 
GCCACACTGA CGCTCACACA CCTGAACAAG GAGGACGAGG GTCTCTACAC CCTGAGAGTC 
ACCACCAAGT CTGGATATGA AACCTACTCC TCCTATGTCT TCGTCAGAGA TTCTGATGCT 
GAGGTGGAAG GAGCTCCAGG AGCCCCTCTG GATGTTCGCT GTCTCGATGC CAACAAGGAC 
TACATCATTG TCACTTGGAA GCAGCCGGCT GTCGATGGTG GCAACTCCAT CATGGGCTAC 
TTTGTGGACA GGTGTGAGGT TGGAACCAAC CACTGGATCC AGTGCAATGA CACTCCAGTC 
AAGTTCGCCC GTTTCCCCGT GACCGGTTTG GTGGAGGGTC GTTCTTATTT CTTCCGCGTG 
CGTGCGGTCA ACAACAGCGG CATGAGTCAT CCGTCCAGAG TGTCTGAACC CGTGGCCGCC 
ATGGACCCGG CTGACCGCGC CCGCTTGAGA GGTACTTCTG CTCCCTGGAC TGGCCAAATC 
ATTGTCACAG AGGAGGAACC TGCAGAGGGC ATTGTTCCTG GCAGACCTCT TGAACTCCAG 
GTGACGGAGG CCACCAAGAA TTACGTAGTG CTGAGCTGGA AGCCACCTGG AGAGAAAGGC 
CTGGAGGGAG TGATGTACTA CGTGGAGAAG TGTGTCTCGG GCACAGACAG CTGGCAGAGG 
GTGAACACAG AGATCCCAGT CAAATCCCCT CGCTTTGCGT TGTTCGATCT GGCCGAGGGG 
AAGTCCTACA GCTTCCGTGT CCGGTGCTGC AACCCTGCCG GGGTCGGTGA ACCCTCTGAC 
CCCACTGAGG CCACCACAGT TGGCGACAAG CTTGATATCC CATCAGCCCC CAGCAAAGTC 
GTCCCCACTA GAAACACAGA CACGTCAGTC GTCGTGTCTT GGGCAGCGTC CAGTGATGCC 
AAAGAGTTGG TTGGTTACTA CATTGAGGGG AGCATCGTGG GCAGCAACGT GTGGGAGCCG 
TGCAACAACA AGCCTGTAAA TGTAACCAGG TTCATCTGCC ACGGTCTGAT GACGGGAGAG 
AAGTACGTGT TCAGGGTGAG GGCAGTGAAC GCAGCAGGAC TCAGCCAGTT CTCCCCAGAG 
TCTGAGCCTG TTGAGGTGAA GGCTGCTATT GCCTCCCCTG CTCCACCCTA TGGCATCAGC 
GTCTTGGAGT GTGTGCGCGA CTCCATGGTG CTGGGCTGGA AACAGCCGAG CTTCATCGGC 
GGTGCTGACA TCACCGGCTA CTTCGTGGAT TACCGCGAGG TCATCGATGG CGTGCCGGAG 
AAGTGGCACG AGGCCAACAT CAAGGCTGTC AGTGAGAGGG CCTACAGAGT GTCCGACCTG 
ATGGAGAACA GGAAGTACCA GTTCCAGGTG CGAGCAGCCA ACATGGCAGG TGTTGGGATC 
CCGTCGCTGG CCAGTGACAC CTTCCTGTGT GAGGAGTGGA CCATCGCTGT GCCAGGACCT 
CCTCATGACC TGCAGGTGAG AGAGGTGCGC AACGACTCTC TGGTATTGCT ATGGAAACCA 
CCTGTGTACC AGGGCCGCGA TCCGGTCAAC GGGTTCTACA TTGACATCAA GGAAGCGGAC 
GCCCCAGAGG AGGCGTGGAG AGGCGTCAAC AACAAGGCCA GGGAGAAGAC GTACATGAAG 
ATTACGAACC TGAAGGAGGG AGAGGTGTAC ATCTTCCGTG TTCGTGCTCA GAATAAAGCC 
GGTGTTGGAA AAACATCTGA CGTGACAGAA CCGGTTTCAG CTGTGACCAA ACCTGGCACT 
AAGGAGATCG TCGTGGACGT AGACGATGAT GGCATCATCT CCCTGAACTT TGAATGCTGT 
GACCTGACCC CTGACTCCAA ATTTGTGTGG TCCAAGAATT ACGAGGAGAT CACAGACCCC 
TCACGCTTGA CTATGGAAAC CAAGGGAAAC AAATCGAAAG CTGTTTTCAA CACTCCTGGA 
GAGGAGGACA TCGGCATTTA CTCCTGTATC GTCACACACA CTGATGGAGC TTCATCCAGC 
TACGATCTCT CTGAGGAAGA GTTGAAGAGG CTGCTGGAGG TCAGTCACGA CCACAAATTC 
CCCATTATTC CCCTGAAGTC ACAGTTGGCT GTGGAACTGC TGGAGAAAGG CAGAGTTCGC 
TTTTGGCTGC AGGCCGAGAA GATTTCTGCT GATGGCAAAG TCGATTACGT GTTTAATGAA 
AATCTCATCT CTCAGGGGGA GAAATATAAG ATGAACTTCG ACAAGAGCAC CGGTGTGATC 
GAGATGATCA TGGAGTCTCT GACCCCAGCG GACGAGGGCA CCTTCACCTT CCAGCTGAAA 
GACGGGAAAG CAACCAACCA GTCCAGCTTG GTGCTGATAG GAAACGTGTT CAAGGAGCTT 
CAGAAGGAAT CAGAGTTCCA GAGGAAAGAA TGGTTCAGAA AGCAAGGTCC TCACTTCATC 
GAGTATTTGA GTTATGAGGT GACGCCCGAG TGCTGCGTTG TGCTGAAATG CAAGGTCGGC 
AACGTGAAGA AGGAAACCTC GGCGCTGTGG TACAAAGACG GACGAGAGAT CAAGGCAGAA 
GAGCATCTGA GCTTCACAGA GGGAGTGTTG AAACTGGAAA TAGCTCAGAT TTCCAAGAGG 
GATTCTGGTG TGTACGAGGT CGTTCTGAAG GACGACAGAG GAAAAGACAC GTCCACATTG 
AATTTAACAG ATCAAGGTTT CAAGGACTTG ATGAATGAAG TTTTCAGTTT TATTGCCAAT 
TCCTCCACTC CACTGAAGAT CACGAGCACA GATGAGGGCA TCCGACTCTA CACCTTCGTC 
AACTTCTATA ACGATTTACT CCAAGTGACA TGGCACTACA AAGAATCAGC CATCGCCTTC 
TCTGACCGTA TAAAGAGCGG CGTGGTGGGG GAGCAGCTGT GGCTGCAGAT CACTGAGCCC 
ACAGAGAAAG ACACGGGCAA GTACGCCATC GAGTTCCACG ACGGAAAGGG AGGCCTGAGG 
AGAACTGTAG AGTTGTCTGG TCAAGCATTT GATGACGCTT TTGCAGAATT CCAGAGACTC 
AAAGCCGCTG CTGTTGCAGA GAGAGGTCGT GCTCGAGTAG CAGGAGGTTT GCCTGATGTG 
GTCACCATCC AGGAGGGCAA GGCCCTCAAT CTCACCTGCA ACATTTCGGG AGACCCCGTG 
CCAAAGGTCA CCTGGCTGAA GAACGACAGG GAAATCACGT CCGACGACCA CTGCATCCTG 
AAGTTCGAGT CCGGCAAGTT CGCCAGTTTC ACCATCACGG CTGTGAACAC GTCGGACACC 
GGAAAATACA GCATCCTGGT GAAGAACAAG TACGGCACAG AGAGCGGGGA CTTCACCCTG 
GAGACCACGG AGGGGACGCC TGACCTGGGC GTGACCTCAT ACTGGACCGA CGCAGATGGA 
AATGTGGTGT TTGGCCCAAA CACTCCCAAG GAAAAACGAA AGGAGTCTAT GGCTAAAGCT 
GCGAAAATGA AGGAAGACAA AAAAGAGAGA AAAAGCGAGG GATCGGAGGA GCAGAAAAAT 
GAGATTAAAG TTGATGCCGC TGCAGAAACA AAAGCTTCTG ATTCAAAACA AACAGGAGAC 
GTAAAGAAAG ATTCAGCCGA CCAATCAGAA ACCAGTGAGC GGCCTGCAGA CACAACGGAG 
GAGCCGGAAC CAGAGGCTGA GACGACACAG CCGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myom1 ( XM_020087597.1 ) cDNA ORF clone, Paralichthys olivaceus(Japanese flounder) -> XP_019943156.1 Paralichthys olivaceus myomesin 1 (myom1), transcript variant X3, mRNA.

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CloneID	OPa240838
Clone ID Related Accession (Same CDS sequence)	XM_020087597.1
Accession Version	XM_020087597.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4704bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-07
Organism	Paralichthys olivaceus(Japanese flounder)
Product	myomesin-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017859653.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Paralichthys olivaceus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAT CTTTACCGTT CTACCAGAAG CACCACCGTC ACTACGACCG TGGCTACCGT 
AACAAGGAGA TGGCATCTAA AATGAGCCAG TACCAGTCCA GCAGCAGCAG CAGCAGCAGA 
TACTTTGCGG GCAGCAGCAC CTCCGCCACC AGCAGCAGCA GCAGCAGAGG ACTTAAAGTG 
TCCTCACACT CTGGGCTGGA AGAGAGCAGA CTGAGCCCCA TACCCAAGAG AACCAAGCCA 
ACTTACTTGG CTGTGGACAA AGAAAACCAA ATCATCGGCT ATGTAGTGCC TATCTTCAGG 
GGCAGTCAAG AGTTTGCTAC AGGATTTTCG GATACAGGCG AATCAAGGGT GAGGGACATG 
GCCCGCAGGG ATTTGTTCAG CAGCGGCCTG GAGATGGAGA GGTCAGAGCA GATCTCCAGG 
AAGCAGGCCA TGCGCGAGTC AGCTGATCGC ATCTCTCTGA GCAAAAGGAT CCATGAACAC 
GAGGAACACT TCAAGCGTAT GAACGAAGAC AGCCTGATGC ACCCCCCGGA GTTTGTGATT 
AAACCTCGCT CCCACACTGT GTGGGAGAAG CAGTGTGTGC GGCTGCACTG CACTGTCAGT 
GGCTGGCCTG ACCCACGGGT CGTCTGGTAC AAAAACAATG TGGCCATTGA CCCCATGGCA 
AACCCTGGCA AGTATAAGCT TGAGAGCAGA TACAGCGTCC ATTCACTGGA TATTAACAGA 
TGCGATTTTG ATGACACAGC TCAGTATCGC GTCTCCGCCA TGAACTCCAA GGGAGAGCTC 
TCTGCATTCG CCTCCATAGT TGTTAAAAGG TTCAAAGGTG AAGTTGATGA GTTCCTGCCA 
GCTCCCAGAC ATGGCCCAGT GTCTGAGTAT GGCATCACCT TCCAGACTCA CATTGTCGAT 
AAGTTTGGCG TGTCATTTGG AAGAGAGGGG GAGACCATGA GTCTGGGGTG CACAGTCGTC 
ATCTACCCGT CCCTGCACCT CTACCAGCCA GAGGTCCAGT GGTACAAAGA TGATGTCCTG 
CTGTCTCCAT CTAAATGGTA CCAAATGCGC TGGAGCGGAG ACAGAGCCAC ACTGACGCTC 
ACACACCTGA ACAAGGAGGA CGAGGGTCTC TACACCCTGA GAGTCACCAC CAAGTCTGGA 
TATGAAACCT ACTCCTCCTA TGTCTTCGTC AGAGATTCTG ATGCTGAGGT GGAAGGAGCT 
CCAGGAGCCC CTCTGGATGT TCGCTGTCTC GATGCCAACA AGGACTACAT CATTGTCACT 
TGGAAGCAGC CGGCTGTCGA TGGTGGCAAC TCCATCATGG GCTACTTTGT GGACAGGTGT 
GAGGTTGGAA CCAACCACTG GATCCAGTGC AATGACACTC CAGTCAAGTT CGCCCGTTTC 
CCCGTGACCG GTTTGGTGGA GGGTCGTTCT TATTTCTTCC GCGTGCGTGC GGTCAACAAC 
AGCGGCATGA GTCATCCGTC CAGAGTGTCT GAACCCGTGG CCGCCATGGA CCCGGCTGAC 
CGCGCCCGCT TGAGAGGTAC TTCTGCTCCC TGGACTGGCC AAATCATTGT CACAGAGGAG 
GAACCTGCAG AGGGCATTGT TCCTGGCAGA CCTCTTGAAC TCCAGGTGAC GGAGGCCACC 
AAGAATTACG TAGTGCTGAG CTGGAAGCCA CCTGGAGAGA AAGGCCTGGA GGGAGTGATG 
TACTACGTGG AGAAGTGTGT CTCGGGCACA GACAGCTGGC AGAGGGTGAA CACAGAGATC 
CCAGTCAAAT CCCCTCGCTT TGCGTTGTTC GATCTGGCCG AGGGGAAGTC CTACAGCTTC 
CGTGTCCGGT GCTGCAACCC TGCCGGGGTC GGTGAACCCT CTGACCCCAC TGAGGCCACC 
ACAGTTGGCG ACAAGCTTGA TATCCCATCA GCCCCCAGCA AAGTCGTCCC CACTAGAAAC 
ACAGACACGT CAGTCGTCGT GTCTTGGGCA GCGTCCAGTG ATGCCAAAGA GTTGGTTGGT 
TACTACATTG AGGGGAGCAT CGTGGGCAGC AACGTGTGGG AGCCGTGCAA CAACAAGCCT 
GTAAATGTAA CCAGGTTCAT CTGCCACGGT CTGATGACGG GAGAGAAGTA CGTGTTCAGG 
GTGAGGGCAG TGAACGCAGC AGGACTCAGC CAGTTCTCCC CAGAGTCTGA GCCTGTTGAG 
GTGAAGGCTG CTATTGCCTC CCCTGCTCCA CCCTATGGCA TCAGCGTCTT GGAGTGTGTG 
CGCGACTCCA TGGTGCTGGG CTGGAAACAG CCGAGCTTCA TCGGCGGTGC TGACATCACC 
GGCTACTTCG TGGATTACCG CGAGGTCATC GATGGCGTGC CGGAGAAGTG GCACGAGGCC 
AACATCAAGG CTGTCAGTGA GAGGGCCTAC AGAGTGTCCG ACCTGATGGA GAACAGGAAG 
TACCAGTTCC AGGTGCGAGC AGCCAACATG GCAGGTGTTG GGATCCCGTC GCTGGCCAGT 
GACACCTTCC TGTGTGAGGA GTGGACCATC GCTGTGCCAG GACCTCCTCA TGACCTGCAG 
GTGAGAGAGG TGCGCAACGA CTCTCTGGTA TTGCTATGGA AACCACCTGT GTACCAGGGC 
CGCGATCCGG TCAACGGGTT CTACATTGAC ATCAAGGAAG CGGACGCCCC AGAGGAGGCG 
TGGAGAGGCG TCAACAACAA GGCCAGGGAG AAGACGTACA TGAAGATTAC GAACCTGAAG 
GAGGGAGAGG TGTACATCTT CCGTGTTCGT GCTCAGAATA AAGCCGGTGT TGGAAAAACA 
TCTGACGTGA CAGAACCGGT TTCAGCTGTG ACCAAACCTG GCACTAAGGA GATCGTCGTG 
GACGTAGACG ATGATGGCAT CATCTCCCTG AACTTTGAAT GCTGTGACCT GACCCCTGAC 
TCCAAATTTG TGTGGTCCAA GAATTACGAG GAGATCACAG ACCCCTCACG CTTGACTATG 
GAAACCAAGG GAAACAAATC GAAAGCTGTT TTCAACACTC CTGGAGAGGA GGACATCGGC 
ATTTACTCCT GTATCGTCAC ACACACTGAT GGAGCTTCAT CCAGCTACGA TCTCTCTGAG 
GAAGAGTTGA AGAGGCTGCT GGAGGTCAGT CACGACCACA AATTCCCCAT TATTCCCCTG 
AAGTCACAGT TGGCTGTGGA ACTGCTGGAG AAAGGCAGAG TTCGCTTTTG GCTGCAGGCC 
GAGAAGATTT CTGCTGATGG CAAAGTCGAT TACGTGTTTA ATGAAAATCT CATCTCTCAG 
GGGGAGAAAT ATAAGATGAA CTTCGACAAG AGCACCGGTG TGATCGAGAT GATCATGGAG 
TCTCTGACCC CAGCGGACGA GGGCACCTTC ACCTTCCAGC TGAAAGACGG GAAAGCAACC 
AACCAGTCCA GCTTGGTGCT GATAGGAAAC GTGTTCAAGG AGCTTCAGAA GGAATCAGAG 
TTCCAGAGGA AAGAATGGTT CAGAAAGCAA GGTCCTCACT TCATCGAGTA TTTGAGTTAT 
GAGGTGACGC CCGAGTGCTG CGTTGTGCTG AAATGCAAGG TCGGCAACGT GAAGAAGGAA 
ACCTCGGCGC TGTGGTACAA AGACGGACGA GAGATCAAGG CAGAAGAGCA TCTGAGCTTC 
ACAGAGGGAG TGTTGAAACT GGAAATAGCT CAGATTTCCA AGAGGGATTC TGGTGTGTAC 
GAGGTCGTTC TGAAGGACGA CAGAGGAAAA GACACGTCCA CATTGAATTT AACAGATCAA 
GGTTTCAAGG ACTTGATGAA TGAAGTTTTC AGTTTTATTG CCAATTCCTC CACTCCACTG 
AAGATCACGA GCACAGATGA GGGCATCCGA CTCTACACCT TCGTCAACTT CTATAACGAT 
TTACTCCAAG TGACATGGCA CTACAAAGAA TCAGCCATCG CCTTCTCTGA CCGTATAAAG 
AGCGGCGTGG TGGGGGAGCA GCTGTGGCTG CAGATCACTG AGCCCACAGA GAAAGACACG 
GGCAAGTACG CCATCGAGTT CCACGACGGA AAGGGAGGCC TGAGGAGAAC TGTAGAGTTG 
TCTGGTCAAG CATTTGATGA CGCTTTTGCA GAATTCCAGA GACTCAAAGC CGCTGCTGTT 
GCAGAGAGAG GTCGTGCTCG AGTAGCAGGA GGTTTGCCTG ATGTGGTCAC CATCCAGGAG 
GGCAAGGCCC TCAATCTCAC CTGCAACATT TCGGGAGACC CCGTGCCAAA GGTCACCTGG 
CTGAAGAACG ACAGGGAAAT CACGTCCGAC GACCACTGCA TCCTGAAGTT CGAGTCCGGC 
AAGTTCGCCA GTTTCACCAT CACGGCTGTG AACACGTCGG ACACCGGAAA ATACAGCATC 
CTGGTGAAGA ACAAGTACGG CACAGAGAGC GGGGACTTCA CCCTGGAGAC CACGGAGGGG 
ACGCCTGACC TGGGCGTGAC CTCATACTGG ACCGACGCAG ATGGAAATGT GGTGTTTGGC 
CCAAACACTC CCAAGGAAAA ACGAAAGGAG TCTATGGCTA AAGCTGCGAA AATGAAGGAA 
GACAAAAAAG AGAGAAAAAG CGAGGGATCG GAGGAGCAGA AAAATGAGAT TAAAGTTGAT 
GCCGCTGCAG AAACAAAAGC TTCTGATTCA AAACAAACAG GAGACGTAAA GAAAGATTCA 
GCCGACCAAT CAGAAACCAG TGAGCGGCCT GCAGACACAA CGGAGGAGCC GGAACCAGAG 
GCTGAGACGA CACAGCCGAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019943156.1
CDS	178..4881
Translation	MSGSLPFYQKHHRHYDRGYRNKEMASKMSQYQSSSSSSSRYFAGSSTSATSSSSSRGLKVSSHSGLEESRLSPIPKRTKPTYLAVDKENQIIGYVVPIFRGSQEFATGFSDTGESRVRDMARRDLFSSGLEMERSEQISRKQAMRESADRISLSKRIHEHEEHFKRMNEDSLMHPPEFVIKPRSHTVWEKQCVRLHCTVSGWPDPRVVWYKNNVAIDPMANPGKYKLESRYSVHSLDINRCDFDDTAQYRVSAMNSKGELSAFASIVVKRFKGEVDEFLPAPRHGPVSEYGITFQTHIVDKFGVSFGREGETMSLGCTVVIYPSLHLYQPEVQWYKDDVLLSPSKWYQMRWSGDRATLTLTHLNKEDEGLYTLRVTTKSGYETYSSYVFVRDSDAEVEGAPGAPLDVRCLDANKDYIIVTWKQPAVDGGNSIMGYFVDRCEVGTNHWIQCNDTPVKFARFPVTGLVEGRSYFFRVRAVNNSGMSHPSRVSEPVAAMDPADRARLRGTSAPWTGQIIVTEEEPAEGIVPGRPLELQVTEATKNYVVLSWKPPGEKGLEGVMYYVEKCVSGTDSWQRVNTEIPVKSPRFALFDLAEGKSYSFRVRCCNPAGVGEPSDPTEATTVGDKLDIPSAPSKVVPTRNTDTSVVVSWAASSDAKELVGYYIEGSIVGSNVWEPCNNKPVNVTRFICHGLMTGEKYVFRVRAVNAAGLSQFSPESEPVEVKAAIASPAPPYGISVLECVRDSMVLGWKQPSFIGGADITGYFVDYREVIDGVPEKWHEANIKAVSERAYRVSDLMENRKYQFQVRAANMAGVGIPSLASDTFLCEEWTIAVPGPPHDLQVREVRNDSLVLLWKPPVYQGRDPVNGFYIDIKEADAPEEAWRGVNNKAREKTYMKITNLKEGEVYIFRVRAQNKAGVGKTSDVTEPVSAVTKPGTKEIVVDVDDDGIISLNFECCDLTPDSKFVWSKNYEEITDPSRLTMETKGNKSKAVFNTPGEEDIGIYSCIVTHTDGASSSYDLSEEELKRLLEVSHDHKFPIIPLKSQLAVELLEKGRVRFWLQAEKISADGKVDYVFNENLISQGEKYKMNFDKSTGVIEMIMESLTPADEGTFTFQLKDGKATNQSSLVLIGNVFKELQKESEFQRKEWFRKQGPHFIEYLSYEVTPECCVVLKCKVGNVKKETSALWYKDGREIKAEEHLSFTEGVLKLEIAQISKRDSGVYEVVLKDDRGKDTSTLNLTDQGFKDLMNEVFSFIANSSTPLKITSTDEGIRLYTFVNFYNDLLQVTWHYKESAIAFSDRIKSGVVGEQLWLQITEPTEKDTGKYAIEFHDGKGGLRRTVELSGQAFDDAFAEFQRLKAAAVAERGRARVAGGLPDVVTIQEGKALNLTCNISGDPVPKVTWLKNDREITSDDHCILKFESGKFASFTITAVNTSDTGKYSILVKNKYGTESGDFTLETTEGTPDLGVTSYWTDADGNVVFGPNTPKEKRKESMAKAAKMKEDKKERKSEGSEEQKNEIKVDAAAETKASDSKQTGDVKKDSADQSETSERPADTTEEPEPEAETTQPK

Target ORF information:

RefSeq Version	XM_020087597.1
Organism	Paralichthys olivaceus(Japanese flounder)
Definition	Paralichthys olivaceus myomesin 1 (myom1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020087597.1

ORF Insert Sequence:

ATGTCTGGAT CTTTACCGTT CTACCAGAAG CACCACCGTC ACTACGACCG TGGCTACCGT 
AACAAGGAGA TGGCATCTAA AATGAGCCAG TACCAGTCCA GCAGCAGCAG CAGCAGCAGA 
TACTTTGCGG GCAGCAGCAC CTCCGCCACC AGCAGCAGCA GCAGCAGAGG ACTTAAAGTG 
TCCTCACACT CTGGGCTGGA AGAGAGCAGA CTGAGCCCCA TACCCAAGAG AACCAAGCCA 
ACTTACTTGG CTGTGGACAA AGAAAACCAA ATCATCGGCT ATGTAGTGCC TATCTTCAGG 
GGCAGTCAAG AGTTTGCTAC AGGATTTTCG GATACAGGCG AATCAAGGGT GAGGGACATG 
GCCCGCAGGG ATTTGTTCAG CAGCGGCCTG GAGATGGAGA GGTCAGAGCA GATCTCCAGG 
AAGCAGGCCA TGCGCGAGTC AGCTGATCGC ATCTCTCTGA GCAAAAGGAT CCATGAACAC 
GAGGAACACT TCAAGCGTAT GAACGAAGAC AGCCTGATGC ACCCCCCGGA GTTTGTGATT 
AAACCTCGCT CCCACACTGT GTGGGAGAAG CAGTGTGTGC GGCTGCACTG CACTGTCAGT 
GGCTGGCCTG ACCCACGGGT CGTCTGGTAC AAAAACAATG TGGCCATTGA CCCCATGGCA 
AACCCTGGCA AGTATAAGCT TGAGAGCAGA TACAGCGTCC ATTCACTGGA TATTAACAGA 
TGCGATTTTG ATGACACAGC TCAGTATCGC GTCTCCGCCA TGAACTCCAA GGGAGAGCTC 
TCTGCATTCG CCTCCATAGT TGTTAAAAGG TTCAAAGGTG AAGTTGATGA GTTCCTGCCA 
GCTCCCAGAC ATGGCCCAGT GTCTGAGTAT GGCATCACCT TCCAGACTCA CATTGTCGAT 
AAGTTTGGCG TGTCATTTGG AAGAGAGGGG GAGACCATGA GTCTGGGGTG CACAGTCGTC 
ATCTACCCGT CCCTGCACCT CTACCAGCCA GAGGTCCAGT GGTACAAAGA TGATGTCCTG 
CTGTCTCCAT CTAAATGGTA CCAAATGCGC TGGAGCGGAG ACAGAGCCAC ACTGACGCTC 
ACACACCTGA ACAAGGAGGA CGAGGGTCTC TACACCCTGA GAGTCACCAC CAAGTCTGGA 
TATGAAACCT ACTCCTCCTA TGTCTTCGTC AGAGATTCTG ATGCTGAGGT GGAAGGAGCT 
CCAGGAGCCC CTCTGGATGT TCGCTGTCTC GATGCCAACA AGGACTACAT CATTGTCACT 
TGGAAGCAGC CGGCTGTCGA TGGTGGCAAC TCCATCATGG GCTACTTTGT GGACAGGTGT 
GAGGTTGGAA CCAACCACTG GATCCAGTGC AATGACACTC CAGTCAAGTT CGCCCGTTTC 
CCCGTGACCG GTTTGGTGGA GGGTCGTTCT TATTTCTTCC GCGTGCGTGC GGTCAACAAC 
AGCGGCATGA GTCATCCGTC CAGAGTGTCT GAACCCGTGG CCGCCATGGA CCCGGCTGAC 
CGCGCCCGCT TGAGAGGTAC TTCTGCTCCC TGGACTGGCC AAATCATTGT CACAGAGGAG 
GAACCTGCAG AGGGCATTGT TCCTGGCAGA CCTCTTGAAC TCCAGGTGAC GGAGGCCACC 
AAGAATTACG TAGTGCTGAG CTGGAAGCCA CCTGGAGAGA AAGGCCTGGA GGGAGTGATG 
TACTACGTGG AGAAGTGTGT CTCGGGCACA GACAGCTGGC AGAGGGTGAA CACAGAGATC 
CCAGTCAAAT CCCCTCGCTT TGCGTTGTTC GATCTGGCCG AGGGGAAGTC CTACAGCTTC 
CGTGTCCGGT GCTGCAACCC TGCCGGGGTC GGTGAACCCT CTGACCCCAC TGAGGCCACC 
ACAGTTGGCG ACAAGCTTGA TATCCCATCA GCCCCCAGCA AAGTCGTCCC CACTAGAAAC 
ACAGACACGT CAGTCGTCGT GTCTTGGGCA GCGTCCAGTG ATGCCAAAGA GTTGGTTGGT 
TACTACATTG AGGGGAGCAT CGTGGGCAGC AACGTGTGGG AGCCGTGCAA CAACAAGCCT 
GTAAATGTAA CCAGGTTCAT CTGCCACGGT CTGATGACGG GAGAGAAGTA CGTGTTCAGG 
GTGAGGGCAG TGAACGCAGC AGGACTCAGC CAGTTCTCCC CAGAGTCTGA GCCTGTTGAG 
GTGAAGGCTG CTATTGCCTC CCCTGCTCCA CCCTATGGCA TCAGCGTCTT GGAGTGTGTG 
CGCGACTCCA TGGTGCTGGG CTGGAAACAG CCGAGCTTCA TCGGCGGTGC TGACATCACC 
GGCTACTTCG TGGATTACCG CGAGGTCATC GATGGCGTGC CGGAGAAGTG GCACGAGGCC 
AACATCAAGG CTGTCAGTGA GAGGGCCTAC AGAGTGTCCG ACCTGATGGA GAACAGGAAG 
TACCAGTTCC AGGTGCGAGC AGCCAACATG GCAGGTGTTG GGATCCCGTC GCTGGCCAGT 
GACACCTTCC TGTGTGAGGA GTGGACCATC GCTGTGCCAG GACCTCCTCA TGACCTGCAG 
GTGAGAGAGG TGCGCAACGA CTCTCTGGTA TTGCTATGGA AACCACCTGT GTACCAGGGC 
CGCGATCCGG TCAACGGGTT CTACATTGAC ATCAAGGAAG CGGACGCCCC AGAGGAGGCG 
TGGAGAGGCG TCAACAACAA GGCCAGGGAG AAGACGTACA TGAAGATTAC GAACCTGAAG 
GAGGGAGAGG TGTACATCTT CCGTGTTCGT GCTCAGAATA AAGCCGGTGT TGGAAAAACA 
TCTGACGTGA CAGAACCGGT TTCAGCTGTG ACCAAACCTG GCACTAAGGA GATCGTCGTG 
GACGTAGACG ATGATGGCAT CATCTCCCTG AACTTTGAAT GCTGTGACCT GACCCCTGAC 
TCCAAATTTG TGTGGTCCAA GAATTACGAG GAGATCACAG ACCCCTCACG CTTGACTATG 
GAAACCAAGG GAAACAAATC GAAAGCTGTT TTCAACACTC CTGGAGAGGA GGACATCGGC 
ATTTACTCCT GTATCGTCAC ACACACTGAT GGAGCTTCAT CCAGCTACGA TCTCTCTGAG 
GAAGAGTTGA AGAGGCTGCT GGAGGTCAGT CACGACCACA AATTCCCCAT TATTCCCCTG 
AAGTCACAGT TGGCTGTGGA ACTGCTGGAG AAAGGCAGAG TTCGCTTTTG GCTGCAGGCC 
GAGAAGATTT CTGCTGATGG CAAAGTCGAT TACGTGTTTA ATGAAAATCT CATCTCTCAG 
GGGGAGAAAT ATAAGATGAA CTTCGACAAG AGCACCGGTG TGATCGAGAT GATCATGGAG 
TCTCTGACCC CAGCGGACGA GGGCACCTTC ACCTTCCAGC TGAAAGACGG GAAAGCAACC 
AACCAGTCCA GCTTGGTGCT GATAGGAAAC GTGTTCAAGG AGCTTCAGAA GGAATCAGAG 
TTCCAGAGGA AAGAATGGTT CAGAAAGCAA GGTCCTCACT TCATCGAGTA TTTGAGTTAT 
GAGGTGACGC CCGAGTGCTG CGTTGTGCTG AAATGCAAGG TCGGCAACGT GAAGAAGGAA 
ACCTCGGCGC TGTGGTACAA AGACGGACGA GAGATCAAGG CAGAAGAGCA TCTGAGCTTC 
ACAGAGGGAG TGTTGAAACT GGAAATAGCT CAGATTTCCA AGAGGGATTC TGGTGTGTAC 
GAGGTCGTTC TGAAGGACGA CAGAGGAAAA GACACGTCCA CATTGAATTT AACAGATCAA 
GGTTTCAAGG ACTTGATGAA TGAAGTTTTC AGTTTTATTG CCAATTCCTC CACTCCACTG 
AAGATCACGA GCACAGATGA GGGCATCCGA CTCTACACCT TCGTCAACTT CTATAACGAT 
TTACTCCAAG TGACATGGCA CTACAAAGAA TCAGCCATCG CCTTCTCTGA CCGTATAAAG 
AGCGGCGTGG TGGGGGAGCA GCTGTGGCTG CAGATCACTG AGCCCACAGA GAAAGACACG 
GGCAAGTACG CCATCGAGTT CCACGACGGA AAGGGAGGCC TGAGGAGAAC TGTAGAGTTG 
TCTGGTCAAG CATTTGATGA CGCTTTTGCA GAATTCCAGA GACTCAAAGC CGCTGCTGTT 
GCAGAGAGAG GTCGTGCTCG AGTAGCAGGA GGTTTGCCTG ATGTGGTCAC CATCCAGGAG 
GGCAAGGCCC TCAATCTCAC CTGCAACATT TCGGGAGACC CCGTGCCAAA GGTCACCTGG 
CTGAAGAACG ACAGGGAAAT CACGTCCGAC GACCACTGCA TCCTGAAGTT CGAGTCCGGC 
AAGTTCGCCA GTTTCACCAT CACGGCTGTG AACACGTCGG ACACCGGAAA ATACAGCATC 
CTGGTGAAGA ACAAGTACGG CACAGAGAGC GGGGACTTCA CCCTGGAGAC CACGGAGGGG 
ACGCCTGACC TGGGCGTGAC CTCATACTGG ACCGACGCAG ATGGAAATGT GGTGTTTGGC 
CCAAACACTC CCAAGGAAAA ACGAAAGGAG TCTATGGCTA AAGCTGCGAA AATGAAGGAA 
GACAAAAAAG AGAGAAAAAG CGAGGGATCG GAGGAGCAGA AAAATGAGAT TAAAGTTGAT 
GCCGCTGCAG AAACAAAAGC TTCTGATTCA AAACAAACAG GAGACGTAAA GAAAGATTCA 
GCCGACCAAT CAGAAACCAG TGAGCGGCCT GCAGACACAA CGGAGGAGCC GGAACCAGAG 
GCTGAGACGA CACAGCCGAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myom1 ( XM_020087598.1 ) cDNA ORF clone, Paralichthys olivaceus(Japanese flounder) -> XP_019943157.1 Paralichthys olivaceus myomesin 1 (myom1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OPa240839
Clone ID Related Accession (Same CDS sequence)	XM_020087598.1
Accession Version	XM_020087598.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4422bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-07
Organism	Paralichthys olivaceus(Japanese flounder)
Product	myomesin-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017859653.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Paralichthys olivaceus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAT CTTTACCGTT CTACCAGAAG CACCACCGTC ACTACGACCG TGGCTACCGT 
AACAAGGAGA TGGCATCTAA AATGAGCCAG TACCAGTCCA GCAGCAGCAG CAGCAGCAGA 
TACTTTGCGG GCAGCAGCAC CTCCGCCACC AGCAGCAGCA GCAGCAGAGG ACTTAAAGTG 
TCCTCACACT CTGGGCTGGA AGAGAGCAGA CTGAGCCCCA TACCCAAGAG AACCAAGCCA 
ACTTACTTGG CTGTGGACAA AGAAAACCAA ATCATCGGCT ATGTAGTGCC TATCTTCAGG 
GGCAGTCAAG AGTTTGCTAC AGGATTTTCG GATACAGGCG AATCAAGGGT GAGGGACATG 
GCCCGCAGGG ATTTGTTCAG CAGCGGCCTG GAGATGGAGA GGTCAGAGCA GATCTCCAGG 
AAGCAGGCCA TGCGCGAGTC AGCTGATCGC ATCTCTCTGA GCAAAAGGAT CCATGAACAC 
GAGGAACACT TCAAGCGTAT GAACGAAGAC AGCCTGATGC ACCCCCCGGA GTTTGTGATT 
AAACCTCGCT CCCACACTGT GTGGGAGAAG CAGTGTGTGC GGCTGCACTG CACTGTCAGT 
GGCTGGCCTG ACCCACGGGT CGTCTGGTAC AAAAACAATG TGGCCATTGA CCCCATGGCA 
AACCCTGGCA AGTATAAGCT TGAGAGCAGA TACAGCGTCC ATTCACTGGA TATTAACAGA 
TGCGATTTTG ATGACACAGC TCAGTATCGC GTCTCCGCCA TGAACTCCAA GGGAGAGCTC 
TCTGCATTCG CCTCCATAGT TGTTAAAAGG TTCAAAGGTG AAGTTGATGA GTTCCTGCCA 
GCTCCCAGAC GGGCCATTAC TAAAGATGGC CCAGTGTCTG AGTATGGCAT CACCTTCCAG 
ACTCACATTG TCGATAAGTT TGGCGTGTCA TTTGGAAGAG AGGGGGAGAC CATGAGTCTG 
GGGTGCACAG TCGTCATCTA CCCGTCCCTG CACCTCTACC AGCCAGAGGT CCAGTGGTAC 
AAAGATGATG TCCTGCTGTC TCCATCTAAA TGGTACCAAA TGCGCTGGAG CGGAGACAGA 
GCCACACTGA CGCTCACACA CCTGAACAAG GAGGACGAGG GTCTCTACAC CCTGAGAGTC 
ACCACCAAGT CTGGATATGA AACCTACTCC TCCTATGTCT TCGTCAGAGA TTCTGATGCT 
GAGGTGGAAG GAGCTCCAGG AGCCCCTCTG GATGTTCGCT GTCTCGATGC CAACAAGGAC 
TACATCATTG TCACTTGGAA GCAGCCGGCT GTCGATGGTG GCAACTCCAT CATGGGCTAC 
TTTGTGGACA GGTGTGAGGT TGGAACCAAC CACTGGATCC AGTGCAATGA CACTCCAGTC 
AAGTTCGCCC GTTTCCCCGT GACCGGTTTG GTGGAGGGTC GTTCTTATTT CTTCCGCGTG 
CGTGCGGTCA ACAACAGCGG CATGAGTCAT CCGTCCAGAG TGTCTGAACC CGTGGCCGCC 
ATGGACCCGG CTGACCGCGC CCGCTTGAGA GGTACTTCTG CTCCCTGGAC TGGCCAAATC 
ATTGTCACAG AGGAGGAACC TGCAGAGGGC ATTGTTCCTG GCAGACCTCT TGAACTCCAG 
GTGACGGAGG CCACCAAGAA TTACGTAGTG CTGAGCTGGA AGCCACCTGG AGAGAAAGGC 
CTGGAGGGAG TGATGTACTA CGTGGAGAAG TGTGTCTCGG GCACAGACAG CTGGCAGAGG 
GTGAACACAG AGATCCCAGT CAAATCCCCT CGCTTTGCGT TGTTCGATCT GGCCGAGGGG 
AAGTCCTACA GCTTCCGTGT CCGGTGCTGC AACCCTGCCG GGGTCGGTGA ACCCTCTGAC 
CCCACTGAGG CCACCACAGT TGGCGACAAG CTTGATATCC CATCAGCCCC CAGCAAAGTC 
GTCCCCACTA GAAACACAGA CACGTCAGTC GTCGTGTCTT GGGCAGCGTC CAGTGATGCC 
AAAGAGTTGG TTGGTTACTA CATTGAGGGG AGCATCGTGG GCAGCAACGT GTGGGAGCCG 
TGCAACAACA AGCCTGTAAA TGTAACCAGG TTCATCTGCC ACGGTCTGAT GACGGGAGAG 
AAGTACGTGT TCAGGGTGAG GGCAGTGAAC GCAGCAGGAC TCAGCCAGTT CTCCCCAGAG 
TCTGAGCCTG TTGAGGTGAA GGCTGCTATT GCCTCCCCTG CTCCACCCTA TGGCATCAGC 
GTCTTGGAGT GTGTGCGCGA CTCCATGGTG CTGGGCTGGA AACAGCCGAG CTTCATCGGC 
GGTGCTGACA TCACCGGCTA CTTCGTGGAT TACCGCGAGG TCATCGATGG CGTGCCGGAG 
AAGTGGCACG AGGCCAACAT CAAGGCTGTC AGTGAGAGGG CCTACAGAGT GTCCGACCTG 
ATGGAGAACA GGAAGTACCA GTTCCAGGTG CGAGCAGCCA ACATGGCAGG TGTTGGGATC 
CCGTCGCTGG CCAGTGACAC CTTCCTGTGT GAGGAGTGGA CCATCGCTGT GCCAGGACCT 
CCTCATGACC TGCAGGTGAG AGAGGTGCGC AACGACTCTC TGGTATTGCT ATGGAAACCA 
CCTGTGTACC AGGGCCGCGA TCCGGTCAAC GGGTTCTACA TTGACATCAA GGAAGCGGAC 
GCCCCAGAGG AGGCGTGGAG AGGCGTCAAC AACAAGGCCA GGGAGAAGAC GTACATGAAG 
ATTACGAACC TGAAGGAGGG AGAGGTGTAC ATCTTCCGTG TTCGTGCTCA GAATAAAGCC 
GGTGTTGGAA AAACATCTGA CGTGACAGAA CCGGTTTCAG CTGTGACCAA ACCTGGCACT 
AAGGAGATCG TCGTGGACGT AGACGATGAT GGCATCATCT CCCTGAACTT TGAATGCTGT 
GACCTGACCC CTGACTCCAA ATTTGTGTGG TCCAAGAATT ACGAGGAGAT CACAGACCCC 
TCACGCTTGA CTATGGAAAC CAAGGGAAAC AAATCGAAAG CTGTTTTCAA CACTCCTGGA 
GAGGAGGACA TCGGCATTTA CTCCTGTATC GTCACACACA CTGATGGAGC TTCATCCAGC 
TACGATCTCT CTGAGGAAGA GTTGAAGAGG CTGCTGGAGG TCAGTCACGA CCACAAATTC 
CCCATTATTC CCCTGAAGTC ACAGTTGGCT GTGGAACTGC TGGAGAAAGG CAGAGTTCGC 
TTTTGGCTGC AGGCCGAGAA GATTTCTGCT GATGGCAAAG TCGATTACGT GTTTAATGAA 
AATCTCATCT CTCAGGGGGA GAAATATAAG ATGAACTTCG ACAAGAGCAC CGGTGTGATC 
GAGATGATCA TGGAGTCTCT GACCCCAGCG GACGAGGGCA CCTTCACCTT CCAGCTGAAA 
GACGGGAAAG CAACCAACCA GTCCAGCTTG GTGCTGATAG GAAACGTGTT CAAGGAGCTT 
CAGAAGGAAT CAGAGTTCCA GAGGAAAGAA TGGTTCAGAA AGCAAGGTCC TCACTTCATC 
GAGTATTTGA GTTATGAGGT GACGCCCGAG TGCTGCGTTG TGCTGAAATG CAAGGTCGGC 
AACGTGAAGA AGGAAACCTC GGCGCTGTGG TACAAAGACG GACGAGAGAT CAAGGCAGAA 
GAGCATCTGA GCTTCACAGA GGGAGTGTTG AAACTGGAAA TAGCTCAGAT TTCCAAGAGG 
GATTCTGGTG TGTACGAGGT CGTTCTGAAG GACGACAGAG GAAAAGACAC GTCCACATTG 
AATTTAACAG ATCAAGGTTT CAAGGACTTG ATGAATGAAG TTTTCAGTTT TATTGCCAAT 
TCCTCCACTC CACTGAAGAT CACGAGCACA GATGAGGGCA TCCGACTCTA CACCTTCGTC 
AACTTCTATA ACGATTTACT CCAAGTGACA TGGCACTACA AAGAATCAGC CATCGCCTTC 
TCTGACCGTA TAAAGAGCGG CGTGGTGGGG GAGCAGCTGT GGCTGCAGAT CACTGAGCCC 
ACAGAGAAAG ACACGGGCAA GTACGCCATC GAGTTCCACG ACGGAAAGGG AGGCCTGAGG 
AGAACTGTAG AGTTGTCTGG TCAAGCATTT GATGACGCTT TTGCAGAATT CCAGAGACTC 
AAAGCCGCTG CTGTTGCAGA GAGAGGTCGT GCTCGAGTAG CAGGAGGTTT GCCTGATGTG 
GTCACCATCC AGGAGGGCAA GGCCCTCAAT CTCACCTGCA ACATTTCGGG AGACCCCGTG 
CCAAAGGTCA CCTGGCTGAA GAACGACAGG GAAATCACGT CCGACGACCA CTGCATCCTG 
AAGTTCGAGT CCGGCAAGTT CGCCAGTTTC ACCATCACGG CTGTGAACAC GTCGGACACC 
GGAAAATACA GCATCCTGGT GAAGAACAAG TACGGCACAG AGAGCGGGGA CTTCACCGTA 
AGTGTCTTCA TCCCTGAAGA GGCGGCCGGC AAGAAAAAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019943157.1
CDS	178..4599
Translation	MSGSLPFYQKHHRHYDRGYRNKEMASKMSQYQSSSSSSSRYFAGSSTSATSSSSSRGLKVSSHSGLEESRLSPIPKRTKPTYLAVDKENQIIGYVVPIFRGSQEFATGFSDTGESRVRDMARRDLFSSGLEMERSEQISRKQAMRESADRISLSKRIHEHEEHFKRMNEDSLMHPPEFVIKPRSHTVWEKQCVRLHCTVSGWPDPRVVWYKNNVAIDPMANPGKYKLESRYSVHSLDINRCDFDDTAQYRVSAMNSKGELSAFASIVVKRFKGEVDEFLPAPRRAITKDGPVSEYGITFQTHIVDKFGVSFGREGETMSLGCTVVIYPSLHLYQPEVQWYKDDVLLSPSKWYQMRWSGDRATLTLTHLNKEDEGLYTLRVTTKSGYETYSSYVFVRDSDAEVEGAPGAPLDVRCLDANKDYIIVTWKQPAVDGGNSIMGYFVDRCEVGTNHWIQCNDTPVKFARFPVTGLVEGRSYFFRVRAVNNSGMSHPSRVSEPVAAMDPADRARLRGTSAPWTGQIIVTEEEPAEGIVPGRPLELQVTEATKNYVVLSWKPPGEKGLEGVMYYVEKCVSGTDSWQRVNTEIPVKSPRFALFDLAEGKSYSFRVRCCNPAGVGEPSDPTEATTVGDKLDIPSAPSKVVPTRNTDTSVVVSWAASSDAKELVGYYIEGSIVGSNVWEPCNNKPVNVTRFICHGLMTGEKYVFRVRAVNAAGLSQFSPESEPVEVKAAIASPAPPYGISVLECVRDSMVLGWKQPSFIGGADITGYFVDYREVIDGVPEKWHEANIKAVSERAYRVSDLMENRKYQFQVRAANMAGVGIPSLASDTFLCEEWTIAVPGPPHDLQVREVRNDSLVLLWKPPVYQGRDPVNGFYIDIKEADAPEEAWRGVNNKAREKTYMKITNLKEGEVYIFRVRAQNKAGVGKTSDVTEPVSAVTKPGTKEIVVDVDDDGIISLNFECCDLTPDSKFVWSKNYEEITDPSRLTMETKGNKSKAVFNTPGEEDIGIYSCIVTHTDGASSSYDLSEEELKRLLEVSHDHKFPIIPLKSQLAVELLEKGRVRFWLQAEKISADGKVDYVFNENLISQGEKYKMNFDKSTGVIEMIMESLTPADEGTFTFQLKDGKATNQSSLVLIGNVFKELQKESEFQRKEWFRKQGPHFIEYLSYEVTPECCVVLKCKVGNVKKETSALWYKDGREIKAEEHLSFTEGVLKLEIAQISKRDSGVYEVVLKDDRGKDTSTLNLTDQGFKDLMNEVFSFIANSSTPLKITSTDEGIRLYTFVNFYNDLLQVTWHYKESAIAFSDRIKSGVVGEQLWLQITEPTEKDTGKYAIEFHDGKGGLRRTVELSGQAFDDAFAEFQRLKAAAVAERGRARVAGGLPDVVTIQEGKALNLTCNISGDPVPKVTWLKNDREITSDDHCILKFESGKFASFTITAVNTSDTGKYSILVKNKYGTESGDFTVSVFIPEEAAGKKK

Target ORF information:

RefSeq Version	XM_020087598.1
Organism	Paralichthys olivaceus(Japanese flounder)
Definition	Paralichthys olivaceus myomesin 1 (myom1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020087598.1

ORF Insert Sequence:

ATGTCTGGAT CTTTACCGTT CTACCAGAAG CACCACCGTC ACTACGACCG TGGCTACCGT 
AACAAGGAGA TGGCATCTAA AATGAGCCAG TACCAGTCCA GCAGCAGCAG CAGCAGCAGA 
TACTTTGCGG GCAGCAGCAC CTCCGCCACC AGCAGCAGCA GCAGCAGAGG ACTTAAAGTG 
TCCTCACACT CTGGGCTGGA AGAGAGCAGA CTGAGCCCCA TACCCAAGAG AACCAAGCCA 
ACTTACTTGG CTGTGGACAA AGAAAACCAA ATCATCGGCT ATGTAGTGCC TATCTTCAGG 
GGCAGTCAAG AGTTTGCTAC AGGATTTTCG GATACAGGCG AATCAAGGGT GAGGGACATG 
GCCCGCAGGG ATTTGTTCAG CAGCGGCCTG GAGATGGAGA GGTCAGAGCA GATCTCCAGG 
AAGCAGGCCA TGCGCGAGTC AGCTGATCGC ATCTCTCTGA GCAAAAGGAT CCATGAACAC 
GAGGAACACT TCAAGCGTAT GAACGAAGAC AGCCTGATGC ACCCCCCGGA GTTTGTGATT 
AAACCTCGCT CCCACACTGT GTGGGAGAAG CAGTGTGTGC GGCTGCACTG CACTGTCAGT 
GGCTGGCCTG ACCCACGGGT CGTCTGGTAC AAAAACAATG TGGCCATTGA CCCCATGGCA 
AACCCTGGCA AGTATAAGCT TGAGAGCAGA TACAGCGTCC ATTCACTGGA TATTAACAGA 
TGCGATTTTG ATGACACAGC TCAGTATCGC GTCTCCGCCA TGAACTCCAA GGGAGAGCTC 
TCTGCATTCG CCTCCATAGT TGTTAAAAGG TTCAAAGGTG AAGTTGATGA GTTCCTGCCA 
GCTCCCAGAC GGGCCATTAC TAAAGATGGC CCAGTGTCTG AGTATGGCAT CACCTTCCAG 
ACTCACATTG TCGATAAGTT TGGCGTGTCA TTTGGAAGAG AGGGGGAGAC CATGAGTCTG 
GGGTGCACAG TCGTCATCTA CCCGTCCCTG CACCTCTACC AGCCAGAGGT CCAGTGGTAC 
AAAGATGATG TCCTGCTGTC TCCATCTAAA TGGTACCAAA TGCGCTGGAG CGGAGACAGA 
GCCACACTGA CGCTCACACA CCTGAACAAG GAGGACGAGG GTCTCTACAC CCTGAGAGTC 
ACCACCAAGT CTGGATATGA AACCTACTCC TCCTATGTCT TCGTCAGAGA TTCTGATGCT 
GAGGTGGAAG GAGCTCCAGG AGCCCCTCTG GATGTTCGCT GTCTCGATGC CAACAAGGAC 
TACATCATTG TCACTTGGAA GCAGCCGGCT GTCGATGGTG GCAACTCCAT CATGGGCTAC 
TTTGTGGACA GGTGTGAGGT TGGAACCAAC CACTGGATCC AGTGCAATGA CACTCCAGTC 
AAGTTCGCCC GTTTCCCCGT GACCGGTTTG GTGGAGGGTC GTTCTTATTT CTTCCGCGTG 
CGTGCGGTCA ACAACAGCGG CATGAGTCAT CCGTCCAGAG TGTCTGAACC CGTGGCCGCC 
ATGGACCCGG CTGACCGCGC CCGCTTGAGA GGTACTTCTG CTCCCTGGAC TGGCCAAATC 
ATTGTCACAG AGGAGGAACC TGCAGAGGGC ATTGTTCCTG GCAGACCTCT TGAACTCCAG 
GTGACGGAGG CCACCAAGAA TTACGTAGTG CTGAGCTGGA AGCCACCTGG AGAGAAAGGC 
CTGGAGGGAG TGATGTACTA CGTGGAGAAG TGTGTCTCGG GCACAGACAG CTGGCAGAGG 
GTGAACACAG AGATCCCAGT CAAATCCCCT CGCTTTGCGT TGTTCGATCT GGCCGAGGGG 
AAGTCCTACA GCTTCCGTGT CCGGTGCTGC AACCCTGCCG GGGTCGGTGA ACCCTCTGAC 
CCCACTGAGG CCACCACAGT TGGCGACAAG CTTGATATCC CATCAGCCCC CAGCAAAGTC 
GTCCCCACTA GAAACACAGA CACGTCAGTC GTCGTGTCTT GGGCAGCGTC CAGTGATGCC 
AAAGAGTTGG TTGGTTACTA CATTGAGGGG AGCATCGTGG GCAGCAACGT GTGGGAGCCG 
TGCAACAACA AGCCTGTAAA TGTAACCAGG TTCATCTGCC ACGGTCTGAT GACGGGAGAG 
AAGTACGTGT TCAGGGTGAG GGCAGTGAAC GCAGCAGGAC TCAGCCAGTT CTCCCCAGAG 
TCTGAGCCTG TTGAGGTGAA GGCTGCTATT GCCTCCCCTG CTCCACCCTA TGGCATCAGC 
GTCTTGGAGT GTGTGCGCGA CTCCATGGTG CTGGGCTGGA AACAGCCGAG CTTCATCGGC 
GGTGCTGACA TCACCGGCTA CTTCGTGGAT TACCGCGAGG TCATCGATGG CGTGCCGGAG 
AAGTGGCACG AGGCCAACAT CAAGGCTGTC AGTGAGAGGG CCTACAGAGT GTCCGACCTG 
ATGGAGAACA GGAAGTACCA GTTCCAGGTG CGAGCAGCCA ACATGGCAGG TGTTGGGATC 
CCGTCGCTGG CCAGTGACAC CTTCCTGTGT GAGGAGTGGA CCATCGCTGT GCCAGGACCT 
CCTCATGACC TGCAGGTGAG AGAGGTGCGC AACGACTCTC TGGTATTGCT ATGGAAACCA 
CCTGTGTACC AGGGCCGCGA TCCGGTCAAC GGGTTCTACA TTGACATCAA GGAAGCGGAC 
GCCCCAGAGG AGGCGTGGAG AGGCGTCAAC AACAAGGCCA GGGAGAAGAC GTACATGAAG 
ATTACGAACC TGAAGGAGGG AGAGGTGTAC ATCTTCCGTG TTCGTGCTCA GAATAAAGCC 
GGTGTTGGAA AAACATCTGA CGTGACAGAA CCGGTTTCAG CTGTGACCAA ACCTGGCACT 
AAGGAGATCG TCGTGGACGT AGACGATGAT GGCATCATCT CCCTGAACTT TGAATGCTGT 
GACCTGACCC CTGACTCCAA ATTTGTGTGG TCCAAGAATT ACGAGGAGAT CACAGACCCC 
TCACGCTTGA CTATGGAAAC CAAGGGAAAC AAATCGAAAG CTGTTTTCAA CACTCCTGGA 
GAGGAGGACA TCGGCATTTA CTCCTGTATC GTCACACACA CTGATGGAGC TTCATCCAGC 
TACGATCTCT CTGAGGAAGA GTTGAAGAGG CTGCTGGAGG TCAGTCACGA CCACAAATTC 
CCCATTATTC CCCTGAAGTC ACAGTTGGCT GTGGAACTGC TGGAGAAAGG CAGAGTTCGC 
TTTTGGCTGC AGGCCGAGAA GATTTCTGCT GATGGCAAAG TCGATTACGT GTTTAATGAA 
AATCTCATCT CTCAGGGGGA GAAATATAAG ATGAACTTCG ACAAGAGCAC CGGTGTGATC 
GAGATGATCA TGGAGTCTCT GACCCCAGCG GACGAGGGCA CCTTCACCTT CCAGCTGAAA 
GACGGGAAAG CAACCAACCA GTCCAGCTTG GTGCTGATAG GAAACGTGTT CAAGGAGCTT 
CAGAAGGAAT CAGAGTTCCA GAGGAAAGAA TGGTTCAGAA AGCAAGGTCC TCACTTCATC 
GAGTATTTGA GTTATGAGGT GACGCCCGAG TGCTGCGTTG TGCTGAAATG CAAGGTCGGC 
AACGTGAAGA AGGAAACCTC GGCGCTGTGG TACAAAGACG GACGAGAGAT CAAGGCAGAA 
GAGCATCTGA GCTTCACAGA GGGAGTGTTG AAACTGGAAA TAGCTCAGAT TTCCAAGAGG 
GATTCTGGTG TGTACGAGGT CGTTCTGAAG GACGACAGAG GAAAAGACAC GTCCACATTG 
AATTTAACAG ATCAAGGTTT CAAGGACTTG ATGAATGAAG TTTTCAGTTT TATTGCCAAT 
TCCTCCACTC CACTGAAGAT CACGAGCACA GATGAGGGCA TCCGACTCTA CACCTTCGTC 
AACTTCTATA ACGATTTACT CCAAGTGACA TGGCACTACA AAGAATCAGC CATCGCCTTC 
TCTGACCGTA TAAAGAGCGG CGTGGTGGGG GAGCAGCTGT GGCTGCAGAT CACTGAGCCC 
ACAGAGAAAG ACACGGGCAA GTACGCCATC GAGTTCCACG ACGGAAAGGG AGGCCTGAGG 
AGAACTGTAG AGTTGTCTGG TCAAGCATTT GATGACGCTT TTGCAGAATT CCAGAGACTC 
AAAGCCGCTG CTGTTGCAGA GAGAGGTCGT GCTCGAGTAG CAGGAGGTTT GCCTGATGTG 
GTCACCATCC AGGAGGGCAA GGCCCTCAAT CTCACCTGCA ACATTTCGGG AGACCCCGTG 
CCAAAGGTCA CCTGGCTGAA GAACGACAGG GAAATCACGT CCGACGACCA CTGCATCCTG 
AAGTTCGAGT CCGGCAAGTT CGCCAGTTTC ACCATCACGG CTGTGAACAC GTCGGACACC 
GGAAAATACA GCATCCTGGT GAAGAACAAG TACGGCACAG AGAGCGGGGA CTTCACCGTA 
AGTGTCTTCA TCCCTGAAGA GGCGGCCGGC AAGAAAAAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myom1 ( XM_020087602.1 ) cDNA ORF clone, Paralichthys olivaceus(Japanese flounder) -> XP_019943161.1 Paralichthys olivaceus myomesin 1 (myom1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OPa240841
Clone ID Related Accession (Same CDS sequence)	XM_020087602.1
Accession Version	XM_020087602.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4407bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-07
Organism	Paralichthys olivaceus(Japanese flounder)
Product	myomesin-1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017859653.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Paralichthys olivaceus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAT CTTTACCGTT CTACCAGAAG CACCACCGTC ACTACGACCG TGGCTACCGT 
AACAAGGAGA TGGCATCTAA AATGAGCCAG TACCAGTCCA GCAGCAGCAG CAGCAGCAGA 
TACTTTGCGG GCAGCAGCAC CTCCGCCACC AGCAGCAGCA GCAGCAGAGG ACTTAAAGTG 
TCCTCACACT CTGGGCTGGA AGAGAGCAGA CTGAGCCCCA TACCCAAGAG AACCAAGCCA 
ACTTACTTGG CTGTGGACAA AGAAAACCAA ATCATCGGCT ATGTAGTGCC TATCTTCAGG 
GGCAGTCAAG AGTTTGCTAC AGGATTTTCG GATACAGGCG AATCAAGGGT GAGGGACATG 
GCCCGCAGGG ATTTGTTCAG CAGCGGCCTG GAGATGGAGA GGTCAGAGCA GATCTCCAGG 
AAGCAGGCCA TGCGCGAGTC AGCTGATCGC ATCTCTCTGA GCAAAAGGAT CCATGAACAC 
GAGGAACACT TCAAGCGTAT GAACGAAGAC AGCCTGATGC ACCCCCCGGA GTTTGTGATT 
AAACCTCGCT CCCACACTGT GTGGGAGAAG CAGTGTGTGC GGCTGCACTG CACTGTCAGT 
GGCTGGCCTG ACCCACGGGT CGTCTGGTAC AAAAACAATG TGGCCATTGA CCCCATGGCA 
AACCCTGGCA AGTATAAGCT TGAGAGCAGA TACAGCGTCC ATTCACTGGA TATTAACAGA 
TGCGATTTTG ATGACACAGC TCAGTATCGC GTCTCCGCCA TGAACTCCAA GGGAGAGCTC 
TCTGCATTCG CCTCCATAGT TGTTAAAAGG TTCAAAGGTG AAGTTGATGA GTTCCTGCCA 
GCTCCCAGAC ATGGCCCAGT GTCTGAGTAT GGCATCACCT TCCAGACTCA CATTGTCGAT 
AAGTTTGGCG TGTCATTTGG AAGAGAGGGG GAGACCATGA GTCTGGGGTG CACAGTCGTC 
ATCTACCCGT CCCTGCACCT CTACCAGCCA GAGGTCCAGT GGTACAAAGA TGATGTCCTG 
CTGTCTCCAT CTAAATGGTA CCAAATGCGC TGGAGCGGAG ACAGAGCCAC ACTGACGCTC 
ACACACCTGA ACAAGGAGGA CGAGGGTCTC TACACCCTGA GAGTCACCAC CAAGTCTGGA 
TATGAAACCT ACTCCTCCTA TGTCTTCGTC AGAGATTCTG ATGCTGAGGT GGAAGGAGCT 
CCAGGAGCCC CTCTGGATGT TCGCTGTCTC GATGCCAACA AGGACTACAT CATTGTCACT 
TGGAAGCAGC CGGCTGTCGA TGGTGGCAAC TCCATCATGG GCTACTTTGT GGACAGGTGT 
GAGGTTGGAA CCAACCACTG GATCCAGTGC AATGACACTC CAGTCAAGTT CGCCCGTTTC 
CCCGTGACCG GTTTGGTGGA GGGTCGTTCT TATTTCTTCC GCGTGCGTGC GGTCAACAAC 
AGCGGCATGA GTCATCCGTC CAGAGTGTCT GAACCCGTGG CCGCCATGGA CCCGGCTGAC 
CGCGCCCGCT TGAGAGGTAC TTCTGCTCCC TGGACTGGCC AAATCATTGT CACAGAGGAG 
GAACCTGCAG AGGGCATTGT TCCTGGCAGA CCTCTTGAAC TCCAGGTGAC GGAGGCCACC 
AAGAATTACG TAGTGCTGAG CTGGAAGCCA CCTGGAGAGA AAGGCCTGGA GGGAGTGATG 
TACTACGTGG AGAAGTGTGT CTCGGGCACA GACAGCTGGC AGAGGGTGAA CACAGAGATC 
CCAGTCAAAT CCCCTCGCTT TGCGTTGTTC GATCTGGCCG AGGGGAAGTC CTACAGCTTC 
CGTGTCCGGT GCTGCAACCC TGCCGGGGTC GGTGAACCCT CTGACCCCAC TGAGGCCACC 
ACAGTTGGCG ACAAGCTTGA TATCCCATCA GCCCCCAGCA AAGTCGTCCC CACTAGAAAC 
ACAGACACGT CAGTCGTCGT GTCTTGGGCA GCGTCCAGTG ATGCCAAAGA GTTGGTTGGT 
TACTACATTG AGGGGAGCAT CGTGGGCAGC AACGTGTGGG AGCCGTGCAA CAACAAGCCT 
GTAAATGTAA CCAGGTTCAT CTGCCACGGT CTGATGACGG GAGAGAAGTA CGTGTTCAGG 
GTGAGGGCAG TGAACGCAGC AGGACTCAGC CAGTTCTCCC CAGAGTCTGA GCCTGTTGAG 
GTGAAGGCTG CTATTGCCTC CCCTGCTCCA CCCTATGGCA TCAGCGTCTT GGAGTGTGTG 
CGCGACTCCA TGGTGCTGGG CTGGAAACAG CCGAGCTTCA TCGGCGGTGC TGACATCACC 
GGCTACTTCG TGGATTACCG CGAGGTCATC GATGGCGTGC CGGAGAAGTG GCACGAGGCC 
AACATCAAGG CTGTCAGTGA GAGGGCCTAC AGAGTGTCCG ACCTGATGGA GAACAGGAAG 
TACCAGTTCC AGGTGCGAGC AGCCAACATG GCAGGTGTTG GGATCCCGTC GCTGGCCAGT 
GACACCTTCC TGTGTGAGGA GTGGACCATC GCTGTGCCAG GACCTCCTCA TGACCTGCAG 
GTGAGAGAGG TGCGCAACGA CTCTCTGGTA TTGCTATGGA AACCACCTGT GTACCAGGGC 
CGCGATCCGG TCAACGGGTT CTACATTGAC ATCAAGGAAG CGGACGCCCC AGAGGAGGCG 
TGGAGAGGCG TCAACAACAA GGCCAGGGAG AAGACGTACA TGAAGATTAC GAACCTGAAG 
GAGGGAGAGG TGTACATCTT CCGTGTTCGT GCTCAGAATA AAGCCGGTGT TGGAAAAACA 
TCTGACGTGA CAGAACCGGT TTCAGCTGTG ACCAAACCTG GCACTAAGGA GATCGTCGTG 
GACGTAGACG ATGATGGCAT CATCTCCCTG AACTTTGAAT GCTGTGACCT GACCCCTGAC 
TCCAAATTTG TGTGGTCCAA GAATTACGAG GAGATCACAG ACCCCTCACG CTTGACTATG 
GAAACCAAGG GAAACAAATC GAAAGCTGTT TTCAACACTC CTGGAGAGGA GGACATCGGC 
ATTTACTCCT GTATCGTCAC ACACACTGAT GGAGCTTCAT CCAGCTACGA TCTCTCTGAG 
GAAGAGTTGA AGAGGCTGCT GGAGGTCAGT CACGACCACA AATTCCCCAT TATTCCCCTG 
AAGTCACAGT TGGCTGTGGA ACTGCTGGAG AAAGGCAGAG TTCGCTTTTG GCTGCAGGCC 
GAGAAGATTT CTGCTGATGG CAAAGTCGAT TACGTGTTTA ATGAAAATCT CATCTCTCAG 
GGGGAGAAAT ATAAGATGAA CTTCGACAAG AGCACCGGTG TGATCGAGAT GATCATGGAG 
TCTCTGACCC CAGCGGACGA GGGCACCTTC ACCTTCCAGC TGAAAGACGG GAAAGCAACC 
AACCAGTCCA GCTTGGTGCT GATAGGAAAC GTGTTCAAGG AGCTTCAGAA GGAATCAGAG 
TTCCAGAGGA AAGAATGGTT CAGAAAGCAA GGTCCTCACT TCATCGAGTA TTTGAGTTAT 
GAGGTGACGC CCGAGTGCTG CGTTGTGCTG AAATGCAAGG TCGGCAACGT GAAGAAGGAA 
ACCTCGGCGC TGTGGTACAA AGACGGACGA GAGATCAAGG CAGAAGAGCA TCTGAGCTTC 
ACAGAGGGAG TGTTGAAACT GGAAATAGCT CAGATTTCCA AGAGGGATTC TGGTGTGTAC 
GAGGTCGTTC TGAAGGACGA CAGAGGAAAA GACACGTCCA CATTGAATTT AACAGATCAA 
GGTTTCAAGG ACTTGATGAA TGAAGTTTTC AGTTTTATTG CCAATTCCTC CACTCCACTG 
AAGATCACGA GCACAGATGA GGGCATCCGA CTCTACACCT TCGTCAACTT CTATAACGAT 
TTACTCCAAG TGACATGGCA CTACAAAGAA TCAGCCATCG CCTTCTCTGA CCGTATAAAG 
AGCGGCGTGG TGGGGGAGCA GCTGTGGCTG CAGATCACTG AGCCCACAGA GAAAGACACG 
GGCAAGTACG CCATCGAGTT CCACGACGGA AAGGGAGGCC TGAGGAGAAC TGTAGAGTTG 
TCTGGTCAAG CATTTGATGA CGCTTTTGCA GAATTCCAGA GACTCAAAGC CGCTGCTGTT 
GCAGAGAGAG GTCGTGCTCG AGTAGCAGGA GGTTTGCCTG ATGTGGTCAC CATCCAGGAG 
GGCAAGGCCC TCAATCTCAC CTGCAACATT TCGGGAGACC CCGTGCCAAA GGTCACCTGG 
CTGAAGAACG ACAGGGAAAT CACGTCCGAC GACCACTGCA TCCTGAAGTT CGAGTCCGGC 
AAGTTCGCCA GTTTCACCAT CACGGCTGTG AACACGTCGG ACACCGGAAA ATACAGCATC 
CTGGTGAAGA ACAAGTACGG CACAGAGAGC GGGGACTTCA CCGTAAGTGT CTTCATCCCT 
GAAGAGGCGG CCGGCAAGAA AAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019943161.1
CDS	177..4583
Translation	MSGSLPFYQKHHRHYDRGYRNKEMASKMSQYQSSSSSSSRYFAGSSTSATSSSSSRGLKVSSHSGLEESRLSPIPKRTKPTYLAVDKENQIIGYVVPIFRGSQEFATGFSDTGESRVRDMARRDLFSSGLEMERSEQISRKQAMRESADRISLSKRIHEHEEHFKRMNEDSLMHPPEFVIKPRSHTVWEKQCVRLHCTVSGWPDPRVVWYKNNVAIDPMANPGKYKLESRYSVHSLDINRCDFDDTAQYRVSAMNSKGELSAFASIVVKRFKGEVDEFLPAPRHGPVSEYGITFQTHIVDKFGVSFGREGETMSLGCTVVIYPSLHLYQPEVQWYKDDVLLSPSKWYQMRWSGDRATLTLTHLNKEDEGLYTLRVTTKSGYETYSSYVFVRDSDAEVEGAPGAPLDVRCLDANKDYIIVTWKQPAVDGGNSIMGYFVDRCEVGTNHWIQCNDTPVKFARFPVTGLVEGRSYFFRVRAVNNSGMSHPSRVSEPVAAMDPADRARLRGTSAPWTGQIIVTEEEPAEGIVPGRPLELQVTEATKNYVVLSWKPPGEKGLEGVMYYVEKCVSGTDSWQRVNTEIPVKSPRFALFDLAEGKSYSFRVRCCNPAGVGEPSDPTEATTVGDKLDIPSAPSKVVPTRNTDTSVVVSWAASSDAKELVGYYIEGSIVGSNVWEPCNNKPVNVTRFICHGLMTGEKYVFRVRAVNAAGLSQFSPESEPVEVKAAIASPAPPYGISVLECVRDSMVLGWKQPSFIGGADITGYFVDYREVIDGVPEKWHEANIKAVSERAYRVSDLMENRKYQFQVRAANMAGVGIPSLASDTFLCEEWTIAVPGPPHDLQVREVRNDSLVLLWKPPVYQGRDPVNGFYIDIKEADAPEEAWRGVNNKAREKTYMKITNLKEGEVYIFRVRAQNKAGVGKTSDVTEPVSAVTKPGTKEIVVDVDDDGIISLNFECCDLTPDSKFVWSKNYEEITDPSRLTMETKGNKSKAVFNTPGEEDIGIYSCIVTHTDGASSSYDLSEEELKRLLEVSHDHKFPIIPLKSQLAVELLEKGRVRFWLQAEKISADGKVDYVFNENLISQGEKYKMNFDKSTGVIEMIMESLTPADEGTFTFQLKDGKATNQSSLVLIGNVFKELQKESEFQRKEWFRKQGPHFIEYLSYEVTPECCVVLKCKVGNVKKETSALWYKDGREIKAEEHLSFTEGVLKLEIAQISKRDSGVYEVVLKDDRGKDTSTLNLTDQGFKDLMNEVFSFIANSSTPLKITSTDEGIRLYTFVNFYNDLLQVTWHYKESAIAFSDRIKSGVVGEQLWLQITEPTEKDTGKYAIEFHDGKGGLRRTVELSGQAFDDAFAEFQRLKAAAVAERGRARVAGGLPDVVTIQEGKALNLTCNISGDPVPKVTWLKNDREITSDDHCILKFESGKFASFTITAVNTSDTGKYSILVKNKYGTESGDFTVSVFIPEEAAGKKK

Target ORF information:

RefSeq Version	XM_020087602.1
Organism	Paralichthys olivaceus(Japanese flounder)
Definition	Paralichthys olivaceus myomesin 1 (myom1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020087602.1

ORF Insert Sequence:

ATGTCTGGAT CTTTACCGTT CTACCAGAAG CACCACCGTC ACTACGACCG TGGCTACCGT 
AACAAGGAGA TGGCATCTAA AATGAGCCAG TACCAGTCCA GCAGCAGCAG CAGCAGCAGA 
TACTTTGCGG GCAGCAGCAC CTCCGCCACC AGCAGCAGCA GCAGCAGAGG ACTTAAAGTG 
TCCTCACACT CTGGGCTGGA AGAGAGCAGA CTGAGCCCCA TACCCAAGAG AACCAAGCCA 
ACTTACTTGG CTGTGGACAA AGAAAACCAA ATCATCGGCT ATGTAGTGCC TATCTTCAGG 
GGCAGTCAAG AGTTTGCTAC AGGATTTTCG GATACAGGCG AATCAAGGGT GAGGGACATG 
GCCCGCAGGG ATTTGTTCAG CAGCGGCCTG GAGATGGAGA GGTCAGAGCA GATCTCCAGG 
AAGCAGGCCA TGCGCGAGTC AGCTGATCGC ATCTCTCTGA GCAAAAGGAT CCATGAACAC 
GAGGAACACT TCAAGCGTAT GAACGAAGAC AGCCTGATGC ACCCCCCGGA GTTTGTGATT 
AAACCTCGCT CCCACACTGT GTGGGAGAAG CAGTGTGTGC GGCTGCACTG CACTGTCAGT 
GGCTGGCCTG ACCCACGGGT CGTCTGGTAC AAAAACAATG TGGCCATTGA CCCCATGGCA 
AACCCTGGCA AGTATAAGCT TGAGAGCAGA TACAGCGTCC ATTCACTGGA TATTAACAGA 
TGCGATTTTG ATGACACAGC TCAGTATCGC GTCTCCGCCA TGAACTCCAA GGGAGAGCTC 
TCTGCATTCG CCTCCATAGT TGTTAAAAGG TTCAAAGGTG AAGTTGATGA GTTCCTGCCA 
GCTCCCAGAC ATGGCCCAGT GTCTGAGTAT GGCATCACCT TCCAGACTCA CATTGTCGAT 
AAGTTTGGCG TGTCATTTGG AAGAGAGGGG GAGACCATGA GTCTGGGGTG CACAGTCGTC 
ATCTACCCGT CCCTGCACCT CTACCAGCCA GAGGTCCAGT GGTACAAAGA TGATGTCCTG 
CTGTCTCCAT CTAAATGGTA CCAAATGCGC TGGAGCGGAG ACAGAGCCAC ACTGACGCTC 
ACACACCTGA ACAAGGAGGA CGAGGGTCTC TACACCCTGA GAGTCACCAC CAAGTCTGGA 
TATGAAACCT ACTCCTCCTA TGTCTTCGTC AGAGATTCTG ATGCTGAGGT GGAAGGAGCT 
CCAGGAGCCC CTCTGGATGT TCGCTGTCTC GATGCCAACA AGGACTACAT CATTGTCACT 
TGGAAGCAGC CGGCTGTCGA TGGTGGCAAC TCCATCATGG GCTACTTTGT GGACAGGTGT 
GAGGTTGGAA CCAACCACTG GATCCAGTGC AATGACACTC CAGTCAAGTT CGCCCGTTTC 
CCCGTGACCG GTTTGGTGGA GGGTCGTTCT TATTTCTTCC GCGTGCGTGC GGTCAACAAC 
AGCGGCATGA GTCATCCGTC CAGAGTGTCT GAACCCGTGG CCGCCATGGA CCCGGCTGAC 
CGCGCCCGCT TGAGAGGTAC TTCTGCTCCC TGGACTGGCC AAATCATTGT CACAGAGGAG 
GAACCTGCAG AGGGCATTGT TCCTGGCAGA CCTCTTGAAC TCCAGGTGAC GGAGGCCACC 
AAGAATTACG TAGTGCTGAG CTGGAAGCCA CCTGGAGAGA AAGGCCTGGA GGGAGTGATG 
TACTACGTGG AGAAGTGTGT CTCGGGCACA GACAGCTGGC AGAGGGTGAA CACAGAGATC 
CCAGTCAAAT CCCCTCGCTT TGCGTTGTTC GATCTGGCCG AGGGGAAGTC CTACAGCTTC 
CGTGTCCGGT GCTGCAACCC TGCCGGGGTC GGTGAACCCT CTGACCCCAC TGAGGCCACC 
ACAGTTGGCG ACAAGCTTGA TATCCCATCA GCCCCCAGCA AAGTCGTCCC CACTAGAAAC 
ACAGACACGT CAGTCGTCGT GTCTTGGGCA GCGTCCAGTG ATGCCAAAGA GTTGGTTGGT 
TACTACATTG AGGGGAGCAT CGTGGGCAGC AACGTGTGGG AGCCGTGCAA CAACAAGCCT 
GTAAATGTAA CCAGGTTCAT CTGCCACGGT CTGATGACGG GAGAGAAGTA CGTGTTCAGG 
GTGAGGGCAG TGAACGCAGC AGGACTCAGC CAGTTCTCCC CAGAGTCTGA GCCTGTTGAG 
GTGAAGGCTG CTATTGCCTC CCCTGCTCCA CCCTATGGCA TCAGCGTCTT GGAGTGTGTG 
CGCGACTCCA TGGTGCTGGG CTGGAAACAG CCGAGCTTCA TCGGCGGTGC TGACATCACC 
GGCTACTTCG TGGATTACCG CGAGGTCATC GATGGCGTGC CGGAGAAGTG GCACGAGGCC 
AACATCAAGG CTGTCAGTGA GAGGGCCTAC AGAGTGTCCG ACCTGATGGA GAACAGGAAG 
TACCAGTTCC AGGTGCGAGC AGCCAACATG GCAGGTGTTG GGATCCCGTC GCTGGCCAGT 
GACACCTTCC TGTGTGAGGA GTGGACCATC GCTGTGCCAG GACCTCCTCA TGACCTGCAG 
GTGAGAGAGG TGCGCAACGA CTCTCTGGTA TTGCTATGGA AACCACCTGT GTACCAGGGC 
CGCGATCCGG TCAACGGGTT CTACATTGAC ATCAAGGAAG CGGACGCCCC AGAGGAGGCG 
TGGAGAGGCG TCAACAACAA GGCCAGGGAG AAGACGTACA TGAAGATTAC GAACCTGAAG 
GAGGGAGAGG TGTACATCTT CCGTGTTCGT GCTCAGAATA AAGCCGGTGT TGGAAAAACA 
TCTGACGTGA CAGAACCGGT TTCAGCTGTG ACCAAACCTG GCACTAAGGA GATCGTCGTG 
GACGTAGACG ATGATGGCAT CATCTCCCTG AACTTTGAAT GCTGTGACCT GACCCCTGAC 
TCCAAATTTG TGTGGTCCAA GAATTACGAG GAGATCACAG ACCCCTCACG CTTGACTATG 
GAAACCAAGG GAAACAAATC GAAAGCTGTT TTCAACACTC CTGGAGAGGA GGACATCGGC 
ATTTACTCCT GTATCGTCAC ACACACTGAT GGAGCTTCAT CCAGCTACGA TCTCTCTGAG 
GAAGAGTTGA AGAGGCTGCT GGAGGTCAGT CACGACCACA AATTCCCCAT TATTCCCCTG 
AAGTCACAGT TGGCTGTGGA ACTGCTGGAG AAAGGCAGAG TTCGCTTTTG GCTGCAGGCC 
GAGAAGATTT CTGCTGATGG CAAAGTCGAT TACGTGTTTA ATGAAAATCT CATCTCTCAG 
GGGGAGAAAT ATAAGATGAA CTTCGACAAG AGCACCGGTG TGATCGAGAT GATCATGGAG 
TCTCTGACCC CAGCGGACGA GGGCACCTTC ACCTTCCAGC TGAAAGACGG GAAAGCAACC 
AACCAGTCCA GCTTGGTGCT GATAGGAAAC GTGTTCAAGG AGCTTCAGAA GGAATCAGAG 
TTCCAGAGGA AAGAATGGTT CAGAAAGCAA GGTCCTCACT TCATCGAGTA TTTGAGTTAT 
GAGGTGACGC CCGAGTGCTG CGTTGTGCTG AAATGCAAGG TCGGCAACGT GAAGAAGGAA 
ACCTCGGCGC TGTGGTACAA AGACGGACGA GAGATCAAGG CAGAAGAGCA TCTGAGCTTC 
ACAGAGGGAG TGTTGAAACT GGAAATAGCT CAGATTTCCA AGAGGGATTC TGGTGTGTAC 
GAGGTCGTTC TGAAGGACGA CAGAGGAAAA GACACGTCCA CATTGAATTT AACAGATCAA 
GGTTTCAAGG ACTTGATGAA TGAAGTTTTC AGTTTTATTG CCAATTCCTC CACTCCACTG 
AAGATCACGA GCACAGATGA GGGCATCCGA CTCTACACCT TCGTCAACTT CTATAACGAT 
TTACTCCAAG TGACATGGCA CTACAAAGAA TCAGCCATCG CCTTCTCTGA CCGTATAAAG 
AGCGGCGTGG TGGGGGAGCA GCTGTGGCTG CAGATCACTG AGCCCACAGA GAAAGACACG 
GGCAAGTACG CCATCGAGTT CCACGACGGA AAGGGAGGCC TGAGGAGAAC TGTAGAGTTG 
TCTGGTCAAG CATTTGATGA CGCTTTTGCA GAATTCCAGA GACTCAAAGC CGCTGCTGTT 
GCAGAGAGAG GTCGTGCTCG AGTAGCAGGA GGTTTGCCTG ATGTGGTCAC CATCCAGGAG 
GGCAAGGCCC TCAATCTCAC CTGCAACATT TCGGGAGACC CCGTGCCAAA GGTCACCTGG 
CTGAAGAACG ACAGGGAAAT CACGTCCGAC GACCACTGCA TCCTGAAGTT CGAGTCCGGC 
AAGTTCGCCA GTTTCACCAT CACGGCTGTG AACACGTCGG ACACCGGAAA ATACAGCATC 
CTGGTGAAGA ACAAGTACGG CACAGAGAGC GGGGACTTCA CCGTAAGTGT CTTCATCCCT 
GAAGAGGCGG CCGGCAAGAA AAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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03-6811-6572

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オンライン注文

myom1 cDNA ORF clone, Paralichthys olivaceus(Japanese flounder)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム