kif18a cDNA ORF clone, Paralichthys olivaceus(Japanese flounder)

Gene
Clones
gRNAs

Summary

Gene Symbol	kif18a
Entrez Gene ID	109625069
Full Name	kinesin family member 18A
Gene Type	protein-coding
Organism	Paralichthys olivaceus(Japanese flounder)

mRNA and Protein(s)

mRNA	Protein	Name
XM_020080135.1	XP_019935694.1	kinesin-like protein KIF18A isoform X1
XM_020080136.1	XP_019935695.1	kinesin-like protein KIF18A isoform X2

Pathways from BioSystems
Gene Ontology

The following kif18a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the kif18a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPa234933	XM_020080135.1 Latest version!	Paralichthys olivaceus kinesin family member 18A (kif18a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OPa234934	XM_020080136.1 Latest version!	Paralichthys olivaceus kinesin family member 18A (kif18a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

kif18a ( XM_020080135.1 ) cDNA ORF clone, Paralichthys olivaceus(Japanese flounder) -> XP_019935694.1 Paralichthys olivaceus kinesin family member 18A (kif18a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPa234933
Clone ID Related Accession (Same CDS sequence)	XM_020080135.1
Accession Version	XM_020080135.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2748bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-07
Organism	Paralichthys olivaceus(Japanese flounder)
Product	kinesin-like protein KIF18A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017859645.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Paralichthys olivaceus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAGTG ACGTGTGTAG CCATGTCAAG GTGGTCATTC GAGTGAGGCC AACCAATGAC 
AGCGAGAAGC GGGAGAACTG CCGAAATGTG GTCCAGGTTG TTGATAACCA CATGCTCATC 
TTTGACCCCA AAGAGCAGGA CTTATCATGT TTTGGGTCTC GAAAAGCACC AAACAGAAAC 
AGAAACATCA ATAAGAAGGC CAACAAGGAC CTTAAATTTG TTTTTGACCA TGTCTTCGAC 
GAGAACTCTA CTCAAGACGA CATATTTGAG AACACCACTA AAGGAGTGTT GGATGGTGTG 
ATGAATGGAT TCAATTGCAC AGTGTTTGCT TATGGTGCTA CTGGAGCAGG AAAGACCCAC 
ACCATGTTAG GCGCACAAAA TGAACCTGGG GTCATGTACC GCACCATGAA AGAGCTGTTT 
AAACGCATGG ATGATGCCAA GGAAGAGAAG GAATTTGCTG TTGCATTCTC ATATCTTGAG 
GTTTACAATG AACAGATCCG AGACCTGTTG GCTAACGCAG GCCCCCTTGC AGTTAGAGAC 
GACGGCTCTA AAGGAGTGGT TGTTCAGGGC CTCACATTGC ATCAGCCCAA ATCTGCAGAG 
CACATTCTTG AGGCTTTGGA TTCTGGCAAT CGAAACAGAA CACAGCACCC AACTGATATG 
AATGCCACCT CTTCTCGGTC CCATGCTGTG TTTCAGATAT ATTTGAGACA GCAGGACAAA 
ACCGCCAGCC TCAACCCAAA CGCCTGTGTT GCCAAGATGA GCCTGATTGA TCTGGCAGGC 
TCGGAGAGAG CCAGCGCCAC AAACGCCAAA GGGGCACGCT TGCGGGAAGG TGCTAACATC 
AATCGCTCAC TACTTGCCCT TGGAAATGTT ATCAACGCCC TTGCTGACCC AAAAAGCAAG 
AAAGCTCATA TCCCATACAG GGACAGCAAG CTGACACGGA TACTGAAGGA CTCCTTGGGA 
GGCAACTGCA GAACGGTTAT GATTGCCAAT GTTGGCCCCT CATCCAAGTC ATATGATGAC 
ACCCACAACA CCCTCAAATA TGCCAACAGG GCCAAGGAGA TAAAGTCATC GCTCAAGAGT 
AATGTTGTAA GCCTGGACAG TCACATTGGC CAGTATGCAG TAATATGTGA GAAGCAACGG 
CAAGAGATTC TACAGTTGAA GAAAAAACTA AAAGAATATG AGGAGAAGAA CATGGTGCCT 
GGGACTTCCA ACACTATCTC CTCTCAGAGA GCAGCTGAGC TCAAAGGGGT GTCAGAAGCT 
CTGCAGCAAA TCTTCTCAAG TCGGACCCAA ATCAGGAGAG AACAGCTGGA ACTGGAGAGA 
CAACTGAAGG AGAACGAGCT GCGCCAGCAC TACAGTGAGG TGGACAACCT GCAGGTCCAA 
ACTTTCTGTG CCAAAGAAAA GACTGAGAAG GCCACTTGTA AGCATGAGCG GAAGATTGCC 
AGCTTAAACA CTCAGCAGCA ACACATTTGT AAGCGTCTGA AGGAGTCAGA AACACGTTTC 
AAGGAAAACG ACGGCTGGCT TCATCGTGTC GAGAATGAGA TGAAGTTGGT GAAGTTAAAT 
GATCAGACTT CAGAGGTGTT AGAGAAGGAA CTACATTGCC ACAGATTGGA GCTGCAAGTA 
AATGACCTCC AAAAATACAT CCATCAGATC ATCGCGCTCA CTACACTGCA GGATCAGGAG 
AACAAGCGAA CTCAGAAAAT GATGGAAATC TTGCTGCCCG CATACAGTCG ACACTACTCT 
GCGTTGCAAC GGGCTGGGCT GGTCACTGCA GCAGATGCGA TGGCAAACGA CGAACTGGAG 
CACCTTGTGT TGAGGGATAG AGGCGTGGTC TGGGCAGACC AGGAGGGGGT GGGGCAGAAA 
GGTGAAAGGA CTGGAGTGGA ATCACAGAAG ACAAATGAGG TGGGAAGCAT CGGGCTCCAT 
GGCGAGTTGG CACCTTTCCT GTCTTTCTCA CATTTGCAAT ACCACCAGAG CAGCCCCTGC 
AGTGCAGAGG GGCAGACAAA AAGAACATCA ATTTGCAAAG CTGCTCTATC CCCAAAAGGT 
AGAGGGAAGG CTGACATGAA ACAGGAACCA CATCCCAGAA TGCCCATCAG GAGAAATCTG 
TCTGCGTCAC TGCCACCACA AAGCCCTCAG TCCGTTGCCA AGGTCCAAGT GTTTGAGGAT 
GGCATGTTTC CGCTCCAGTG CACACCAGAA CCTGCTCGAA AAACTGCTGA GGCGCAGTCG 
CTGAGCTCTT CCACTGCTAT GGATCCCAAC ATGACCTTCG AAGTTCTGGA CAATGATGAT 
CAAACTGCAA ATAATGCAAC CATCCTATTA AGCGGTGATA GTCCCTCTCA ACCATCCAAG 
TCCAATGACG TCTCGGGTCA CAGCCGACTA CACCATGCAC CTAAGGCCAA TGAGGGGAAG 
CAGATTCAGA CTAAAAGACA GCAGATTCCA TCTTTACAAG AGGTCAGACG AGCCAAGCCG 
ACCTACATGG AAATGACGTC TGCTGCGCAA GGAAAGCGTA AATTTAATCG CAGCATCAGA 
GAAGATATAA CTCAGGGTCT CTCTGCACCA AAGCGTATAA AGAAAGACCC CTTGGTGGCC 
CAGAGACCGC TCAGAGTGCA TCGCTTCGCT GGTTCAGAAG AAAACAAGCC TGGTAGAGTT 
GTAAGGAGCA TTTCTGAGGG GAACCTCAAC CTCCTCGAAC CTCAGAAATC CAAGTCCATT 
TTCTACAGAA CAACCCAACA GATAAAACGA GTGGCCAAGC GGATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019935694.1
CDS	175..2922
Translation	MASDVCSHVKVVIRVRPTNDSEKRENCRNVVQVVDNHMLIFDPKEQDLSCFGSRKAPNRNRNINKKANKDLKFVFDHVFDENSTQDDIFENTTKGVLDGVMNGFNCTVFAYGATGAGKTHTMLGAQNEPGVMYRTMKELFKRMDDAKEEKEFAVAFSYLEVYNEQIRDLLANAGPLAVRDDGSKGVVVQGLTLHQPKSAEHILEALDSGNRNRTQHPTDMNATSSRSHAVFQIYLRQQDKTASLNPNACVAKMSLIDLAGSERASATNAKGARLREGANINRSLLALGNVINALADPKSKKAHIPYRDSKLTRILKDSLGGNCRTVMIANVGPSSKSYDDTHNTLKYANRAKEIKSSLKSNVVSLDSHIGQYAVICEKQRQEILQLKKKLKEYEEKNMVPGTSNTISSQRAAELKGVSEALQQIFSSRTQIRREQLELERQLKENELRQHYSEVDNLQVQTFCAKEKTEKATCKHERKIASLNTQQQHICKRLKESETRFKENDGWLHRVENEMKLVKLNDQTSEVLEKELHCHRLELQVNDLQKYIHQIIALTTLQDQENKRTQKMMEILLPAYSRHYSALQRAGLVTAADAMANDELEHLVLRDRGVVWADQEGVGQKGERTGVESQKTNEVGSIGLHGELAPFLSFSHLQYHQSSPCSAEGQTKRTSICKAALSPKGRGKADMKQEPHPRMPIRRNLSASLPPQSPQSVAKVQVFEDGMFPLQCTPEPARKTAEAQSLSSSTAMDPNMTFEVLDNDDQTANNATILLSGDSPSQPSKSNDVSGHSRLHHAPKANEGKQIQTKRQQIPSLQEVRRAKPTYMEMTSAAQGKRKFNRSIREDITQGLSAPKRIKKDPLVAQRPLRVHRFAGSEENKPGRVVRSISEGNLNLLEPQKSKSIFYRTTQQIKRVAKRM

Target ORF information:

RefSeq Version	XM_020080135.1
Organism	Paralichthys olivaceus(Japanese flounder)
Definition	Paralichthys olivaceus kinesin family member 18A (kif18a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020080135.1

ORF Insert Sequence:

ATGGCAAGTG ACGTGTGTAG CCATGTCAAG GTGGTCATTC GAGTGAGGCC AACCAATGAC 
AGCGAGAAGC GGGAGAACTG CCGAAATGTG GTCCAGGTTG TTGATAACCA CATGCTCATC 
TTTGACCCCA AAGAGCAGGA CTTATCATGT TTTGGGTCTC GAAAAGCACC AAACAGAAAC 
AGAAACATCA ATAAGAAGGC CAACAAGGAC CTTAAATTTG TTTTTGACCA TGTCTTCGAC 
GAGAACTCTA CTCAAGACGA CATATTTGAG AACACCACTA AAGGAGTGTT GGATGGTGTG 
ATGAATGGAT TCAATTGCAC AGTGTTTGCT TATGGTGCTA CTGGAGCAGG AAAGACCCAC 
ACCATGTTAG GCGCACAAAA TGAACCTGGG GTCATGTACC GCACCATGAA AGAGCTGTTT 
AAACGCATGG ATGATGCCAA GGAAGAGAAG GAATTTGCTG TTGCATTCTC ATATCTTGAG 
GTTTACAATG AACAGATCCG AGACCTGTTG GCTAACGCAG GCCCCCTTGC AGTTAGAGAC 
GACGGCTCTA AAGGAGTGGT TGTTCAGGGC CTCACATTGC ATCAGCCCAA ATCTGCAGAG 
CACATTCTTG AGGCTTTGGA TTCTGGCAAT CGAAACAGAA CACAGCACCC AACTGATATG 
AATGCCACCT CTTCTCGGTC CCATGCTGTG TTTCAGATAT ATTTGAGACA GCAGGACAAA 
ACCGCCAGCC TCAACCCAAA CGCCTGTGTT GCCAAGATGA GCCTGATTGA TCTGGCAGGC 
TCGGAGAGAG CCAGCGCCAC AAACGCCAAA GGGGCACGCT TGCGGGAAGG TGCTAACATC 
AATCGCTCAC TACTTGCCCT TGGAAATGTT ATCAACGCCC TTGCTGACCC AAAAAGCAAG 
AAAGCTCATA TCCCATACAG GGACAGCAAG CTGACACGGA TACTGAAGGA CTCCTTGGGA 
GGCAACTGCA GAACGGTTAT GATTGCCAAT GTTGGCCCCT CATCCAAGTC ATATGATGAC 
ACCCACAACA CCCTCAAATA TGCCAACAGG GCCAAGGAGA TAAAGTCATC GCTCAAGAGT 
AATGTTGTAA GCCTGGACAG TCACATTGGC CAGTATGCAG TAATATGTGA GAAGCAACGG 
CAAGAGATTC TACAGTTGAA GAAAAAACTA AAAGAATATG AGGAGAAGAA CATGGTGCCT 
GGGACTTCCA ACACTATCTC CTCTCAGAGA GCAGCTGAGC TCAAAGGGGT GTCAGAAGCT 
CTGCAGCAAA TCTTCTCAAG TCGGACCCAA ATCAGGAGAG AACAGCTGGA ACTGGAGAGA 
CAACTGAAGG AGAACGAGCT GCGCCAGCAC TACAGTGAGG TGGACAACCT GCAGGTCCAA 
ACTTTCTGTG CCAAAGAAAA GACTGAGAAG GCCACTTGTA AGCATGAGCG GAAGATTGCC 
AGCTTAAACA CTCAGCAGCA ACACATTTGT AAGCGTCTGA AGGAGTCAGA AACACGTTTC 
AAGGAAAACG ACGGCTGGCT TCATCGTGTC GAGAATGAGA TGAAGTTGGT GAAGTTAAAT 
GATCAGACTT CAGAGGTGTT AGAGAAGGAA CTACATTGCC ACAGATTGGA GCTGCAAGTA 
AATGACCTCC AAAAATACAT CCATCAGATC ATCGCGCTCA CTACACTGCA GGATCAGGAG 
AACAAGCGAA CTCAGAAAAT GATGGAAATC TTGCTGCCCG CATACAGTCG ACACTACTCT 
GCGTTGCAAC GGGCTGGGCT GGTCACTGCA GCAGATGCGA TGGCAAACGA CGAACTGGAG 
CACCTTGTGT TGAGGGATAG AGGCGTGGTC TGGGCAGACC AGGAGGGGGT GGGGCAGAAA 
GGTGAAAGGA CTGGAGTGGA ATCACAGAAG ACAAATGAGG TGGGAAGCAT CGGGCTCCAT 
GGCGAGTTGG CACCTTTCCT GTCTTTCTCA CATTTGCAAT ACCACCAGAG CAGCCCCTGC 
AGTGCAGAGG GGCAGACAAA AAGAACATCA ATTTGCAAAG CTGCTCTATC CCCAAAAGGT 
AGAGGGAAGG CTGACATGAA ACAGGAACCA CATCCCAGAA TGCCCATCAG GAGAAATCTG 
TCTGCGTCAC TGCCACCACA AAGCCCTCAG TCCGTTGCCA AGGTCCAAGT GTTTGAGGAT 
GGCATGTTTC CGCTCCAGTG CACACCAGAA CCTGCTCGAA AAACTGCTGA GGCGCAGTCG 
CTGAGCTCTT CCACTGCTAT GGATCCCAAC ATGACCTTCG AAGTTCTGGA CAATGATGAT 
CAAACTGCAA ATAATGCAAC CATCCTATTA AGCGGTGATA GTCCCTCTCA ACCATCCAAG 
TCCAATGACG TCTCGGGTCA CAGCCGACTA CACCATGCAC CTAAGGCCAA TGAGGGGAAG 
CAGATTCAGA CTAAAAGACA GCAGATTCCA TCTTTACAAG AGGTCAGACG AGCCAAGCCG 
ACCTACATGG AAATGACGTC TGCTGCGCAA GGAAAGCGTA AATTTAATCG CAGCATCAGA 
GAAGATATAA CTCAGGGTCT CTCTGCACCA AAGCGTATAA AGAAAGACCC CTTGGTGGCC 
CAGAGACCGC TCAGAGTGCA TCGCTTCGCT GGTTCAGAAG AAAACAAGCC TGGTAGAGTT 
GTAAGGAGCA TTTCTGAGGG GAACCTCAAC CTCCTCGAAC CTCAGAAATC CAAGTCCATT 
TTCTACAGAA CAACCCAACA GATAAAACGA GTGGCCAAGC GGATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif18a ( XM_020080136.1 ) cDNA ORF clone, Paralichthys olivaceus(Japanese flounder) -> XP_019935695.1 Paralichthys olivaceus kinesin family member 18A (kif18a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPa234934
Clone ID Related Accession (Same CDS sequence)	XM_020080136.1
Accession Version	XM_020080136.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2745bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-07
Organism	Paralichthys olivaceus(Japanese flounder)
Product	kinesin-like protein KIF18A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017859645.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Paralichthys olivaceus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAGTG ACGTGTGTAG CCATGTCAAG GTGGTCATTC GAGTGAGGCC AACCAATGAC 
AGCGAGAAGC GGGAGAACTG CCGAAATGTG GTCCAGGTTG TTGATAACCA CATGCTCATC 
TTTGACCCCA AAGAGCAGGA CTTATCATGT TTTGGGTCTC GAAAAGCACC AAACAGAAAC 
AGAAACATCA ATAAGAAGGC CAACAAGGAC CTTAAATTTG TTTTTGACCA TGTCTTCGAC 
GAGAACTCTA CTCAAGACGA CATATTTGAG AACACCACTA AAGGAGTGTT GGATGGTGTG 
ATGAATGGAT TCAATTGCAC AGTGTTTGCT TATGGTGCTA CTGGAGCAGG AAAGACCCAC 
ACCATGTTAG GCGCACAAAA TGAACCTGGG GTCATGTACC GCACCATGAA AGAGCTGTTT 
AAACGCATGG ATGATGCCAA GGAAGAGAAG GAATTTGCTG TTGCATTCTC ATATCTTGAG 
GTTTACAATG AACAGATCCG AGACCTGTTG GCTAACGCAG GCCCCCTTGC AGTTAGAGAC 
GACGGCTCTA AAGGAGTGGT TGTTCAGGGC CTCACATTGC ATCAGCCCAA ATCTGCAGAG 
CACATTCTTG AGGCTTTGGA TTCTGGCAAT CGAAACAGAA CACAGCACCC AACTGATATG 
AATGCCACCT CTTCTCGGTC CCATGCTGTG TTTCAGATAT ATTTGAGACA GCAGGACAAA 
ACCGCCAGCC TCAACCCAAA CGCCTGTGTT GCCAAGATGA GCCTGATTGA TCTGGCAGGC 
TCGGAGAGAG CCAGCGCCAC AAACGCCAAA GGGGCACGCT TGCGGGAAGG TGCTAACATC 
AATCGCTCAC TACTTGCCCT TGGAAATGTT ATCAACGCCC TTGCTGACCC AAAAAGCAAG 
AAAGCTCATA TCCCATACAG GGACAGCAAG CTGACACGGA TACTGAAGGA CTCCTTGGGA 
GGCAACTGCA GAACGGTTAT GATTGCCAAT GTTGGCCCCT CATCCAAGTC ATATGATGAC 
ACCCACAACA CCCTCAAATA TGCCAACAGG GCCAAGGAGA TAAAGTCATC GCTCAAGAGT 
AATGTTGTAA GCCTGGACAG TCACATTGGC CAGTATGCAG TAATATGTGA GAAGCAACGG 
CAAGAGATTC TACAGTTGAA GAAAAAACTA AAAGAATATG AGGAGAAGAA CATGGTGCCT 
GGGACTTCCA ACACTATCTC CTCTCAGAGA GCAGCTGAGC TCAAAGGGGT GTCAGAAGCT 
CTGCAGCAAA TCTTCTCAAG TCGGACCCAA ATCAGGAGAG AACAGCTGGA ACTGGAGAGA 
CAACTGAAGG AGAACGAGCT GCGCCAGCAC TACAGTGAGG TGGACAACCT GCAGGTCCAA 
ACTTTCTGTG CCAAAGAAAA GACTGAGAAG GCCACTTGTA AGCATGAGCG GAAGATTGCC 
AGCTTAAACA CTCAGCAGCA ACACATTTGT AAGCGTCTGA AGGAGTCAGA AACACGTTTC 
AAGGAAAACG ACGGCTGGCT TCATCGTGTC GAGAATGAGA TGAAGTTGGT GAAGTTAAAT 
GATCAGACTT CAGAGGTGTT AGAGAAGGAA CTACATTGCC ACAGATTGGA GCTGCAAGTA 
AATGACCTCC AAAAATACAT CCATCAGATC ATCGCGCTCA CTACACTGCA GGATCAGGAG 
AACAAGCGAA CTCAGAAAAT GATGGAAATC TTGCTGCCCG CATACAGTCG ACACTACTCT 
GCGTTGCAAC GGGCTGGGCT GGTCACTGCA GCAGATGCGA TGGCAAACGA CGAACTGGAG 
CACCTTGTGT TGAGGGATAG AGGCGTGGTC TGGGCAGACC AGGAGGGGGT GGGGCAGAAA 
GGTGAAAGGA CTGGAGTGGA ATCACAGAAG ACAAATGAGG TGGGAAGCAT CGGGCTCCAT 
GGCGAGTTGG CACCTTTCCT GTCTTTCTCA CATTTGCAAT ACCACCAGAG CAGCCCCTGC 
AGTGCAGAGG GGCAGACAAA AAGAACATCA ATTTGCAAAG CTGCTCTATC CCCAAAAGGT 
AGAGGGAAGG CTGACATGAA ACAGGAACCA CATCCCAGAA TGCCCATCAG GAGAAATCTG 
TCTGCGTCAC TGCCACCACA AAGCCCTCAG TCCGTTGCCA AGGTCCAAGT GTTTGAGGAT 
GGCATGTTTC CGCTCCAGTG CACACCAGAA CCTGCTCGAA AAACTGCTGA GGCGCAGTCG 
CTGAGCTCTT CCACTGCTAT GGATCCCAAC ATGACCTTCG AAGTTCTGGA CAATGATGAT 
CAAACTGCAA ATAATGCAAC CATCCTATTA AGCGGTGATA GTCCCTCTCA ACCATCCAAG 
TCCAATGACG TCTCGGGTCA CAGCCGACTA CACCATGCAC CTAAGGCCAA TGAGGGGAAG 
CAGATTCAGA CTAAAAGACA GCAGATTCCA TCTTTACAAG AGGTCAGACG AGCCAAGCCG 
ACCTACATGG AAATGACGTC TGCTGCGCAA GGAAAGCGTA AATTTAATCG CATCAGAGAA 
GATATAACTC AGGGTCTCTC TGCACCAAAG CGTATAAAGA AAGACCCCTT GGTGGCCCAG 
AGACCGCTCA GAGTGCATCG CTTCGCTGGT TCAGAAGAAA ACAAGCCTGG TAGAGTTGTA 
AGGAGCATTT CTGAGGGGAA CCTCAACCTC CTCGAACCTC AGAAATCCAA GTCCATTTTC 
TACAGAACAA CCCAACAGAT AAAACGAGTG GCCAAGCGGA TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019935695.1
CDS	1..2745
Translation	MASDVCSHVKVVIRVRPTNDSEKRENCRNVVQVVDNHMLIFDPKEQDLSCFGSRKAPNRNRNINKKANKDLKFVFDHVFDENSTQDDIFENTTKGVLDGVMNGFNCTVFAYGATGAGKTHTMLGAQNEPGVMYRTMKELFKRMDDAKEEKEFAVAFSYLEVYNEQIRDLLANAGPLAVRDDGSKGVVVQGLTLHQPKSAEHILEALDSGNRNRTQHPTDMNATSSRSHAVFQIYLRQQDKTASLNPNACVAKMSLIDLAGSERASATNAKGARLREGANINRSLLALGNVINALADPKSKKAHIPYRDSKLTRILKDSLGGNCRTVMIANVGPSSKSYDDTHNTLKYANRAKEIKSSLKSNVVSLDSHIGQYAVICEKQRQEILQLKKKLKEYEEKNMVPGTSNTISSQRAAELKGVSEALQQIFSSRTQIRREQLELERQLKENELRQHYSEVDNLQVQTFCAKEKTEKATCKHERKIASLNTQQQHICKRLKESETRFKENDGWLHRVENEMKLVKLNDQTSEVLEKELHCHRLELQVNDLQKYIHQIIALTTLQDQENKRTQKMMEILLPAYSRHYSALQRAGLVTAADAMANDELEHLVLRDRGVVWADQEGVGQKGERTGVESQKTNEVGSIGLHGELAPFLSFSHLQYHQSSPCSAEGQTKRTSICKAALSPKGRGKADMKQEPHPRMPIRRNLSASLPPQSPQSVAKVQVFEDGMFPLQCTPEPARKTAEAQSLSSSTAMDPNMTFEVLDNDDQTANNATILLSGDSPSQPSKSNDVSGHSRLHHAPKANEGKQIQTKRQQIPSLQEVRRAKPTYMEMTSAAQGKRKFNRIREDITQGLSAPKRIKKDPLVAQRPLRVHRFAGSEENKPGRVVRSISEGNLNLLEPQKSKSIFYRTTQQIKRVAKRM

Target ORF information:

RefSeq Version	XM_020080136.1
Organism	Paralichthys olivaceus(Japanese flounder)
Definition	Paralichthys olivaceus kinesin family member 18A (kif18a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020080136.1

ORF Insert Sequence:

ATGGCAAGTG ACGTGTGTAG CCATGTCAAG GTGGTCATTC GAGTGAGGCC AACCAATGAC 
AGCGAGAAGC GGGAGAACTG CCGAAATGTG GTCCAGGTTG TTGATAACCA CATGCTCATC 
TTTGACCCCA AAGAGCAGGA CTTATCATGT TTTGGGTCTC GAAAAGCACC AAACAGAAAC 
AGAAACATCA ATAAGAAGGC CAACAAGGAC CTTAAATTTG TTTTTGACCA TGTCTTCGAC 
GAGAACTCTA CTCAAGACGA CATATTTGAG AACACCACTA AAGGAGTGTT GGATGGTGTG 
ATGAATGGAT TCAATTGCAC AGTGTTTGCT TATGGTGCTA CTGGAGCAGG AAAGACCCAC 
ACCATGTTAG GCGCACAAAA TGAACCTGGG GTCATGTACC GCACCATGAA AGAGCTGTTT 
AAACGCATGG ATGATGCCAA GGAAGAGAAG GAATTTGCTG TTGCATTCTC ATATCTTGAG 
GTTTACAATG AACAGATCCG AGACCTGTTG GCTAACGCAG GCCCCCTTGC AGTTAGAGAC 
GACGGCTCTA AAGGAGTGGT TGTTCAGGGC CTCACATTGC ATCAGCCCAA ATCTGCAGAG 
CACATTCTTG AGGCTTTGGA TTCTGGCAAT CGAAACAGAA CACAGCACCC AACTGATATG 
AATGCCACCT CTTCTCGGTC CCATGCTGTG TTTCAGATAT ATTTGAGACA GCAGGACAAA 
ACCGCCAGCC TCAACCCAAA CGCCTGTGTT GCCAAGATGA GCCTGATTGA TCTGGCAGGC 
TCGGAGAGAG CCAGCGCCAC AAACGCCAAA GGGGCACGCT TGCGGGAAGG TGCTAACATC 
AATCGCTCAC TACTTGCCCT TGGAAATGTT ATCAACGCCC TTGCTGACCC AAAAAGCAAG 
AAAGCTCATA TCCCATACAG GGACAGCAAG CTGACACGGA TACTGAAGGA CTCCTTGGGA 
GGCAACTGCA GAACGGTTAT GATTGCCAAT GTTGGCCCCT CATCCAAGTC ATATGATGAC 
ACCCACAACA CCCTCAAATA TGCCAACAGG GCCAAGGAGA TAAAGTCATC GCTCAAGAGT 
AATGTTGTAA GCCTGGACAG TCACATTGGC CAGTATGCAG TAATATGTGA GAAGCAACGG 
CAAGAGATTC TACAGTTGAA GAAAAAACTA AAAGAATATG AGGAGAAGAA CATGGTGCCT 
GGGACTTCCA ACACTATCTC CTCTCAGAGA GCAGCTGAGC TCAAAGGGGT GTCAGAAGCT 
CTGCAGCAAA TCTTCTCAAG TCGGACCCAA ATCAGGAGAG AACAGCTGGA ACTGGAGAGA 
CAACTGAAGG AGAACGAGCT GCGCCAGCAC TACAGTGAGG TGGACAACCT GCAGGTCCAA 
ACTTTCTGTG CCAAAGAAAA GACTGAGAAG GCCACTTGTA AGCATGAGCG GAAGATTGCC 
AGCTTAAACA CTCAGCAGCA ACACATTTGT AAGCGTCTGA AGGAGTCAGA AACACGTTTC 
AAGGAAAACG ACGGCTGGCT TCATCGTGTC GAGAATGAGA TGAAGTTGGT GAAGTTAAAT 
GATCAGACTT CAGAGGTGTT AGAGAAGGAA CTACATTGCC ACAGATTGGA GCTGCAAGTA 
AATGACCTCC AAAAATACAT CCATCAGATC ATCGCGCTCA CTACACTGCA GGATCAGGAG 
AACAAGCGAA CTCAGAAAAT GATGGAAATC TTGCTGCCCG CATACAGTCG ACACTACTCT 
GCGTTGCAAC GGGCTGGGCT GGTCACTGCA GCAGATGCGA TGGCAAACGA CGAACTGGAG 
CACCTTGTGT TGAGGGATAG AGGCGTGGTC TGGGCAGACC AGGAGGGGGT GGGGCAGAAA 
GGTGAAAGGA CTGGAGTGGA ATCACAGAAG ACAAATGAGG TGGGAAGCAT CGGGCTCCAT 
GGCGAGTTGG CACCTTTCCT GTCTTTCTCA CATTTGCAAT ACCACCAGAG CAGCCCCTGC 
AGTGCAGAGG GGCAGACAAA AAGAACATCA ATTTGCAAAG CTGCTCTATC CCCAAAAGGT 
AGAGGGAAGG CTGACATGAA ACAGGAACCA CATCCCAGAA TGCCCATCAG GAGAAATCTG 
TCTGCGTCAC TGCCACCACA AAGCCCTCAG TCCGTTGCCA AGGTCCAAGT GTTTGAGGAT 
GGCATGTTTC CGCTCCAGTG CACACCAGAA CCTGCTCGAA AAACTGCTGA GGCGCAGTCG 
CTGAGCTCTT CCACTGCTAT GGATCCCAAC ATGACCTTCG AAGTTCTGGA CAATGATGAT 
CAAACTGCAA ATAATGCAAC CATCCTATTA AGCGGTGATA GTCCCTCTCA ACCATCCAAG 
TCCAATGACG TCTCGGGTCA CAGCCGACTA CACCATGCAC CTAAGGCCAA TGAGGGGAAG 
CAGATTCAGA CTAAAAGACA GCAGATTCCA TCTTTACAAG AGGTCAGACG AGCCAAGCCG 
ACCTACATGG AAATGACGTC TGCTGCGCAA GGAAAGCGTA AATTTAATCG CATCAGAGAA 
GATATAACTC AGGGTCTCTC TGCACCAAAG CGTATAAAGA AAGACCCCTT GGTGGCCCAG 
AGACCGCTCA GAGTGCATCG CTTCGCTGGT TCAGAAGAAA ACAAGCCTGG TAGAGTTGTA 
AGGAGCATTT CTGAGGGGAA CCTCAACCTC CTCGAACCTC AGAAATCCAA GTCCATTTTC 
TACAGAACAA CCCAACAGAT AAAACGAGTG GCCAAGCGGA TGTAA