MSTN cDNA ORF clone, Ovis aries(sheep)

Gene
Clones
gRNAs

Summary

Gene Symbol	MSTN
Entrez Gene ID	443449
Full Name	myostatin
Synonyms	GDF-8,GDF8
Gene Type	protein-coding
Organism	Ovis aries(sheep)
Summary	This gene encodes a member of the TGF-beta (transforming growth factor-beta) family of proteins. The encoded preproprotein is proteolytically processed to generate an N-terminal propeptide and a C-terminal subunit. The C-terminal subunits form the mature homodimer, which negatively regulates skeletal muscle cell proliferation and differentiation. Mutations in this gene are associated with increased skeletal muscle mass in humans and other mammals. [provided by RefSeq, Jun 2016].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001009428.3	NP_001009428.1	growth/differentiation factor 8 precursor
NM_001009428.2	NP_001009428.1	growth/differentiation factor 8 precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Multiplex gene editing via CRISPR/Cas9 exhibits desirable muscle hypertrophy without detectable off-target effects in sheep.
Wang X, Niu Y, Zhou J, Yu H, Kou Q, Lei A, Zhao X, Yan H, Cai B, Shen Q, Zhou S, Zhu H, Zhou G, Niu W, Hua J, Jiang Y, Huang X, Ma B, Chen Y
Scientific reports632271(2016 Aug)

Two single nucleotide polymorphisms in the promoter of the ovine myostatin gene (MSTN) and their effect on growth and carcass muscle traits in New Zealand Romney sheep.
Wang J, Zhou H, Hu J, Li S, Luo Y, Hickford JG
Journal of animal breeding and genetics = Zeitschrift fur Tierzuchtung und Zuchtungsbiologie133(3)219-26(2016 Jun)

Efficient Generation of Myostatin Knock-Out Sheep Using CRISPR/Cas9 Technology and Microinjection into Zygotes.
Crispo M, Mulet AP, Tesson L, Barrera N, Cuadro F, dos Santos-Neto PC, Nguyen TH, Cr?n?guy A, Brusselle L, Aneg?n I, Menchaca A
PloS one10(8)e0136690(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The effect of these SNPs on growth and carcass traits was investigated in 357 New Zealand Romney lambs in addition to 2 previously identified SNPs, c.-2379C was associated with increased birthweight, tailing weight and weaning weight.
Title: Two single nucleotide polymorphisms in the promoter of the ovine myostatin gene (MSTN) and their effect on growth and carcass muscle traits in New Zealand Romney sheep.

Data show that Yes-associated protein 1 (YAP1) expression was significantly or and positively correlated with (MSTN) and myogenin (MyoG) at 2 days old, 2 and 6 months old.
Title: Correlation between sheep YAP1 temporal and spatial expression trends and MSTN and MyoG gene expression.

we demonstrate zygote injection of TALEN mRNA can also produce gene-edited cattle and sheep. In both species we have targeted the myostatin (MSTN) gene.
Title: Genome edited sheep and cattle.

We determined the efficiency of the CRISPR/Cas9 system to edit MSTN in sheep and generate knock-out (KO) animals with the aim to promote muscle development and body growth
Title: Efficient Generation of Myostatin Knock-Out Sheep Using CRISPR/Cas9 Technology and Microinjection into Zygotes.

The mRNA expression levels of MSTN, IGF-1 and IGF-2 were significantly different in the same skeletal muscle under different dietary intake.
Title: Effects of different dietary intake on mRNA levels of MSTN, IGF-I, and IGF-II in the skeletal muscle of Dorper and Hu sheep hybrid F1 rams.

The following MSTN gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MSTN cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSm00226	NM_001009428.3 Latest version!	Ovis aries myostatin (MSTN), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
View Old Accession Versions >>
OSm00226	NM_001009428.2	Ovis aries myostatin (MSTN), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MSTN ( NM_001009428.3 ) cDNA ORF clone, Ovis aries(sheep) -> NP_001009428.1 Ovis aries myostatin (MSTN), mRNA.

Price & Availability↑

CloneID	OSm00226
Clone ID Related Accession (Same CDS sequence)	NM_001009428.3 , NM_001009428.2
Accession Version	NM_001009428.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1128bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-25
Organism	Ovis aries(sheep)
Product	growth/differentiation factor 8 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from PEKD01000915.1. On Apr 24, 2019 this sequence version replaced NM_001009428.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: KM371731.1, SRR8173312.177299.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA104494974, SAMEA104495017 [ECO:0000348] ##Evidence-Data-END##

ATGCAAAAAC TGCAAATCTT TGTTTATATT TACCTATTTA TGCTGCTTGT TGCTGGCCCA 
GTGGATCTGA ATGAGAACAG CGAGCAGAAG GAAAATGTGG AAAAAAAGGG GCTGTGTAAT 
GCATGCTTGT GGAGACAAAA CAATAAATCC TCAAGACTAG AAGCCATAAA AATCCAAATC 
CTCAGTAAGC TTCGCCTGGA AACAGCTCCT AACATCAGCA AAGATGCTAT AAGACAACTT 
TTGCCCAAGG CTCCTCCACT CCGGGAACTG ATTGATCAGT ACGATGTCCA GAGAGATGAC 
AGCAGCGACG GCTCCTTGGA AGACGATGAC TACCACGTTA CGACGGAAAC GGTCATTACC 
ATGCCCACGG AGTCTGATCT TCTAGCAGAA GTGCAAGAAA AACCCAAATG TTGCTTCTTT 
AAATTTAGCT CTAAGATACA ACACAATAAA GTAGTAAAGG CCCAACTGTG GATATATCTG 
AGACCTGTCA AGACTCCTAC AACAGTGTTT GTGCAAATCC TGAGACTCAT CAAACCCATG 
AAAGACGGTA CAAGGTATAC TGGAATCCGA TCTCTGAAAC TTGACATGAA CCCAGGCACT 
GGTATTTGGC AGAGCATTGA TGTGAAGACA GTGTTGCAAA ACTGGCTCAA ACAACCTGAA 
TCCAACTTAG GCATTGAAAT CAAAGCTTTA GATGAGAATG GTCATGATCT TGCTGTAACC 
TTCCCAGAAC CAGGAGAAGA AGGACTGAAT CCTTTTTTAG AAGTCAAGGT AACAGACACA 
CCAAAAAGAT CTAGGAGAGA TTTTGGGCTT GATTGTGATG AGCACTCCAC AGAATCTCGA 
TGCTGTCGTT ACCCTCTAAC TGTGGATTTT GAAGCTTTTG GATGGGATTG GATTATTGCA 
CCTAAAAGAT ATAAGGCCAA TTACTGCTCT GGAGAATGTG AATTTTTATT TTTGCAAAAG 
TATCCTCATA CCCATCTTGT GCACCAAGCA AACCCCAAAG GTTCAGCCGG CCCTTGCTGT 
ACTCCTACAA AGATGTCTCC AATTAATATG CTATATTTTA ATGGCAAAGA ACAAATAATA 
TATGGGAAGA TTCCAGGCAT GGTAGTAGAT CGCTGTGGGT GCTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001009428.1
CDS	209..1336
Translation	MQKLQIFVYIYLFMLLVAGPVDLNENSEQKENVEKKGLCNACLWRQNNKSSRLEAIKIQILSKLRLETAPNISKDAIRQLLPKAPPLRELIDQYDVQRDDSSDGSLEDDDYHVTTETVITMPTESDLLAEVQEKPKCCFFKFSSKIQHNKVVKAQLWIYLRPVKTPTTVFVQILRLIKPMKDGTRYTGIRSLKLDMNPGTGIWQSIDVKTVLQNWLKQPESNLGIEIKALDENGHDLAVTFPEPGEEGLNPFLEVKVTDTPKRSRRDFGLDCDEHSTESRCCRYPLTVDFEAFGWDWIIAPKRYKANYCSGECEFLFLQKYPHTHLVHQANPKGSAGPCCTPTKMSPINMLYFNGKEQIIYGKIPGMVVDRCGCS

Target ORF information:

RefSeq Version	NM_001009428.3
Organism	Ovis aries(sheep)
Definition	Ovis aries myostatin (MSTN), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001009428.3

ORF Insert Sequence:

ATGCAAAAAC TGCAAATCTT TGTTTATATT TACCTATTTA TGCTGCTTGT TGCTGGCCCA 
GTGGATCTGA ATGAGAACAG CGAGCAGAAG GAAAATGTGG AAAAAAAGGG GCTGTGTAAT 
GCATGCTTGT GGAGACAAAA CAATAAATCC TCAAGACTAG AAGCCATAAA AATCCAAATC 
CTCAGTAAGC TTCGCCTGGA AACAGCTCCT AACATCAGCA AAGATGCTAT AAGACAACTT 
TTGCCCAAGG CTCCTCCACT CCGGGAACTG ATTGATCAGT ACGATGTCCA GAGAGATGAC 
AGCAGCGACG GCTCCTTGGA AGACGATGAC TACCACGTTA CGACGGAAAC GGTCATTACC 
ATGCCCACGG AGTCTGATCT TCTAGCAGAA GTGCAAGAAA AACCCAAATG TTGCTTCTTT 
AAATTTAGCT CTAAGATACA ACACAATAAA GTAGTAAAGG CCCAACTGTG GATATATCTG 
AGACCTGTCA AGACTCCTAC AACAGTGTTT GTGCAAATCC TGAGACTCAT CAAACCCATG 
AAAGACGGTA CAAGGTATAC TGGAATCCGA TCTCTGAAAC TTGACATGAA CCCAGGCACT 
GGTATTTGGC AGAGCATTGA TGTGAAGACA GTGTTGCAAA ACTGGCTCAA ACAACCTGAA 
TCCAACTTAG GCATTGAAAT CAAAGCTTTA GATGAGAATG GTCATGATCT TGCTGTAACC 
TTCCCAGAAC CAGGAGAAGA AGGACTGAAT CCTTTTTTAG AAGTCAAGGT AACAGACACA 
CCAAAAAGAT CTAGGAGAGA TTTTGGGCTT GATTGTGATG AGCACTCCAC AGAATCTCGA 
TGCTGTCGTT ACCCTCTAAC TGTGGATTTT GAAGCTTTTG GATGGGATTG GATTATTGCA 
CCTAAAAGAT ATAAGGCCAA TTACTGCTCT GGAGAATGTG AATTTTTATT TTTGCAAAAG 
TATCCTCATA CCCATCTTGT GCACCAAGCA AACCCCAAAG GTTCAGCCGG CCCTTGCTGT 
ACTCCTACAA AGATGTCTCC AATTAATATG CTATATTTTA ATGGCAAAGA ACAAATAATA 
TATGGGAAGA TTCCAGGCAT GGTAGTAGAT CGCTGTGGGT GCTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MSTN ( NM_001009428.2 ) cDNA ORF clone, Ovis aries(sheep) -> NP_001009428.1 Ovis aries myostatin (MSTN), mRNA.

Price & Availability↑

CloneID	OSm00226
Clone ID Related Accession (Same CDS sequence)	NM_001009428.3 , NM_001009428.2
Accession Version	NM_001009428.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1128bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Ovis aries(sheep)
Product	growth/differentiation factor 8 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from KM371731.1 and AMGL02007816.1. On Jun 30, 2016 this sequence version replaced NM_001009428.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: KM371731.1, AM992883.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA104494974, SAMEA104495017 [ECO:0000348] ##Evidence-Data-END##

ATGCAAAAAC TGCAAATCTT TGTTTATATT TACCTATTTA TGCTGCTTGT TGCTGGCCCA 
GTGGATCTGA ATGAGAACAG CGAGCAGAAG GAAAATGTGG AAAAAAAGGG GCTGTGTAAT 
GCATGCTTGT GGAGACAAAA CAATAAATCC TCAAGACTAG AAGCCATAAA AATCCAAATC 
CTCAGTAAGC TTCGCCTGGA AACAGCTCCT AACATCAGCA AAGATGCTAT AAGACAACTT 
TTGCCCAAGG CTCCTCCACT CCGGGAACTG ATTGATCAGT ACGATGTCCA GAGAGATGAC 
AGCAGCGACG GCTCCTTGGA AGACGATGAC TACCACGTTA CGACGGAAAC GGTCATTACC 
ATGCCCACGG AGTCTGATCT TCTAGCAGAA GTGCAAGAAA AACCCAAATG TTGCTTCTTT 
AAATTTAGCT CTAAGATACA ACACAATAAA GTAGTAAAGG CCCAACTGTG GATATATCTG 
AGACCTGTCA AGACTCCTAC AACAGTGTTT GTGCAAATCC TGAGACTCAT CAAACCCATG 
AAAGACGGTA CAAGGTATAC TGGAATCCGA TCTCTGAAAC TTGACATGAA CCCAGGCACT 
GGTATTTGGC AGAGCATTGA TGTGAAGACA GTGTTGCAAA ACTGGCTCAA ACAACCTGAA 
TCCAACTTAG GCATTGAAAT CAAAGCTTTA GATGAGAATG GTCATGATCT TGCTGTAACC 
TTCCCAGAAC CAGGAGAAGA AGGACTGAAT CCTTTTTTAG AAGTCAAGGT AACAGACACA 
CCAAAAAGAT CTAGGAGAGA TTTTGGGCTT GATTGTGATG AGCACTCCAC AGAATCTCGA 
TGCTGTCGTT ACCCTCTAAC TGTGGATTTT GAAGCTTTTG GATGGGATTG GATTATTGCA 
CCTAAAAGAT ATAAGGCCAA TTACTGCTCT GGAGAATGTG AATTTTTATT TTTGCAAAAG 
TATCCTCATA CCCATCTTGT GCACCAAGCA AACCCCAAAG GTTCAGCCGG CCCTTGCTGT 
ACTCCTACAA AGATGTCTCC AATTAATATG CTATATTTTA ATGGCAAAGA ACAAATAATA 
TATGGGAAGA TTCCAGGCAT GGTAGTAGAT CGCTGTGGGT GCTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001009428.1
CDS	209..1336
Translation	MQKLQIFVYIYLFMLLVAGPVDLNENSEQKENVEKKGLCNACLWRQNNKSSRLEAIKIQILSKLRLETAPNISKDAIRQLLPKAPPLRELIDQYDVQRDDSSDGSLEDDDYHVTTETVITMPTESDLLAEVQEKPKCCFFKFSSKIQHNKVVKAQLWIYLRPVKTPTTVFVQILRLIKPMKDGTRYTGIRSLKLDMNPGTGIWQSIDVKTVLQNWLKQPESNLGIEIKALDENGHDLAVTFPEPGEEGLNPFLEVKVTDTPKRSRRDFGLDCDEHSTESRCCRYPLTVDFEAFGWDWIIAPKRYKANYCSGECEFLFLQKYPHTHLVHQANPKGSAGPCCTPTKMSPINMLYFNGKEQIIYGKIPGMVVDRCGCS

Target ORF information:

RefSeq Version	NM_001009428.2
Organism	Ovis aries(sheep)
Definition	Ovis aries myostatin (MSTN), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001009428.2

ORF Insert Sequence:

ATGCAAAAAC TGCAAATCTT TGTTTATATT TACCTATTTA TGCTGCTTGT TGCTGGCCCA 
GTGGATCTGA ATGAGAACAG CGAGCAGAAG GAAAATGTGG AAAAAAAGGG GCTGTGTAAT 
GCATGCTTGT GGAGACAAAA CAATAAATCC TCAAGACTAG AAGCCATAAA AATCCAAATC 
CTCAGTAAGC TTCGCCTGGA AACAGCTCCT AACATCAGCA AAGATGCTAT AAGACAACTT 
TTGCCCAAGG CTCCTCCACT CCGGGAACTG ATTGATCAGT ACGATGTCCA GAGAGATGAC 
AGCAGCGACG GCTCCTTGGA AGACGATGAC TACCACGTTA CGACGGAAAC GGTCATTACC 
ATGCCCACGG AGTCTGATCT TCTAGCAGAA GTGCAAGAAA AACCCAAATG TTGCTTCTTT 
AAATTTAGCT CTAAGATACA ACACAATAAA GTAGTAAAGG CCCAACTGTG GATATATCTG 
AGACCTGTCA AGACTCCTAC AACAGTGTTT GTGCAAATCC TGAGACTCAT CAAACCCATG 
AAAGACGGTA CAAGGTATAC TGGAATCCGA TCTCTGAAAC TTGACATGAA CCCAGGCACT 
GGTATTTGGC AGAGCATTGA TGTGAAGACA GTGTTGCAAA ACTGGCTCAA ACAACCTGAA 
TCCAACTTAG GCATTGAAAT CAAAGCTTTA GATGAGAATG GTCATGATCT TGCTGTAACC 
TTCCCAGAAC CAGGAGAAGA AGGACTGAAT CCTTTTTTAG AAGTCAAGGT AACAGACACA 
CCAAAAAGAT CTAGGAGAGA TTTTGGGCTT GATTGTGATG AGCACTCCAC AGAATCTCGA 
TGCTGTCGTT ACCCTCTAAC TGTGGATTTT GAAGCTTTTG GATGGGATTG GATTATTGCA 
CCTAAAAGAT ATAAGGCCAA TTACTGCTCT GGAGAATGTG AATTTTTATT TTTGCAAAAG 
TATCCTCATA CCCATCTTGT GCACCAAGCA AACCCCAAAG GTTCAGCCGG CCCTTGCTGT 
ACTCCTACAA AGATGTCTCC AATTAATATG CTATATTTTA ATGGCAAAGA ACAAATAATA 
TATGGGAAGA TTCCAGGCAT GGTAGTAGAT CGCTGTGGGT GCTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Multiplex gene editing via CRISPR/Cas9 exhibits desirable muscle hypertrophy without detectable off-target effects in sheep.
Scientific reports632271(2016 Aug)
Wang X,Niu Y,Zhou J,Yu H,Kou Q,Lei A,Zhao X,Yan H,Cai B,Shen Q,Zhou S,Zhu H,Zhou G,Niu W,Hua J,Jiang Y,Huang X,Ma B,Chen Y

Two single nucleotide polymorphisms in the promoter of the ovine myostatin gene (MSTN) and their effect on growth and carcass muscle traits in New Zealand Romney sheep.
Journal of animal breeding and genetics = Zeitschrift fur Tierzuchtung und Zuchtungsbiologie133(3)219-26(2016 Jun)
Wang J,Zhou H,Hu J,Li S,Luo Y,Hickford JG

Efficient Generation of Myostatin Knock-Out Sheep Using CRISPR/Cas9 Technology and Microinjection into Zygotes.
PloS one10(8)e0136690(2015)
Crispo M,Mulet AP,Tesson L,Barrera N,Cuadro F,dos Santos-Neto PC,Nguyen TH,Cr?n?guy A,Brusselle L,Aneg?n I,Menchaca A

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MSTN cDNA ORF clone, Ovis aries(sheep)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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