IL4 cDNA ORF clone, Ovis aries(sheep)

Gene
Clones
gRNAs

Summary

Gene Symbol	IL4
Entrez Gene ID	443327
Full Name	interleukin 4
Synonyms	IL-4
Gene Type	protein-coding
Organism	Ovis aries(sheep)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001009313.2	NP_001009313.2	interleukin-4 precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Early IL-4 gene expression in abomasum is associated with resistance to Haemonchus contortus in hair and wool sheep breeds.
Jacobs JR, Sommers KN, Zajac AM, Notter DR, Bowdridge SA
Parasite immunology38(6)333-9(2016 06)

Prolongation of sheep corneal allograft survival by transfer of the gene encoding ovine IL-12-p40 but not IL-4 to donor corneal endothelium.
Klebe S, Coster DJ, Sykes PJ, Swinburne S, Hallsworth P, Scheerlinck JP, Krishnan R, Williams KA
Journal of immunology (Baltimore, Md. : 1950)175(4)2219-26(2005 Aug)

Generation and characterization of ovine dendritic cells derived from peripheral blood monocytes.
Chan SS, McConnell I, Blacklaws BA
Immunology107(3)366-72(2002 Nov)

The expression and biologic effects of ovine interleukin-4 on T and B cell proliferation.
Chaplin PJ, Casey G, De Rose R, Buchan G, Wood PR, Scheerlinck JP
Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research20(4)419-25(2000 Apr)

Cloning and sequencing an ovine interleukin-4-encoding cDNA.
Seow HF, Rothel JS, Wood PR
Gene124(2)291-3(1993 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Early IL-4 gene expression in abomasum is associated with resistance to Haemonchus infection.
Title: Early IL-4 gene expression in abomasum is associated with resistance to Haemonchus contortus in hair and wool sheep breeds.

Local production of the prototypic immunomodulatory cytokine IL-4 in transplanted corneal endothelium induces eosinophilia, inflammation, and rejection.
Title: Prolongation of sheep corneal allograft survival by transfer of the gene encoding ovine IL-12-p40 but not IL-4 to donor corneal endothelium.

The following IL4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IL4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSm00211	NM_001009313.2 Latest version!	Ovis aries interleukin 4 (IL4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

IL4 ( NM_001009313.2 ) cDNA ORF clone, Ovis aries(sheep) -> NP_001009313.2 Ovis aries interleukin 4 (IL4), mRNA.

Price & Availability↑

CloneID	OSm00211
Clone ID Related Accession (Same CDS sequence)	NM_001009313.2
Accession Version	NM_001009313.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 408bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-28
Organism	Ovis aries(sheep)
Product	interleukin-4 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from M96845.1. On Jan 12, 2005 this sequence version replaced NM_001009313.1. ##Evidence-Data-START## Transcript exon combination :: M96845.1 [ECO:0000332] ##Evidence-Data-END##

ATGGGTCTCA CCTCCCAGCT GATCCCAGCG CTGGTCTGCT TACTGGTATG TACCAGCCAC 
TTCGTCCATG GACACAAGTG TGACATTACC TTAGAAGAGA TCATCAAAAC GCCGAACATC 
CTCACATCGC AGAAGAATTC ATGCATGGAG CTGCCTGCAG CAGACGTCTT TGCTGCCCCA 
AAGAACGCAA CTGAGAAGGA AACCTTCTGT AGGACTGGAA TTGAGCTTAG GCGTATCTAC 
AGGAGCCACA TGTGCTTGAA CAAATTCCTG GGCGGACTTG ACAGGAATCT CAGCAGCCTG 
GCAAGCAAGA CCTGTTCTGT GAATGAAGCC AAGACGAGTA CAAGTACGCT GAGAGACCTC 
TTGGAAAGGC TAAAGACTAT TATGAGGGAG AAATACTCAA AGTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001009313.2
CDS	59..466
Translation	MGLTSQLIPALVCLLVCTSHFVHGHKCDITLEEIIKTPNILTSQKNSCMELPAADVFAAPKNATEKETFCRTGIELRRIYRSHMCLNKFLGGLDRNLSSLASKTCSVNEAKTSTSTLRDLLERLKTIMREKYSKC

Target ORF information:

RefSeq Version	NM_001009313.2
Organism	Ovis aries(sheep)
Definition	Ovis aries interleukin 4 (IL4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001009313.2

ORF Insert Sequence:

ATGGGTCTCA CCTCCCAGCT GATCCCAGCG CTGGTCTGCT TACTGGTATG TACCAGCCAC 
TTCGTCCATG GACACAAGTG TGACATTACC TTAGAAGAGA TCATCAAAAC GCCGAACATC 
CTCACATCGC AGAAGAATTC ATGCATGGAG CTGCCTGCAG CAGACGTCTT TGCTGCCCCA 
AAGAACGCAA CTGAGAAGGA AACCTTCTGT AGGACTGGAA TTGAGCTTAG GCGTATCTAC 
AGGAGCCACA TGTGCTTGAA CAAATTCCTG GGCGGACTTG ACAGGAATCT CAGCAGCCTG 
GCAAGCAAGA CCTGTTCTGT GAATGAAGCC AAGACGAGTA CAAGTACGCT GAGAGACCTC 
TTGGAAAGGC TAAAGACTAT TATGAGGGAG AAATACTCAA AGTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Early IL-4 gene expression in abomasum is associated with resistance to Haemonchus contortus in hair and wool sheep breeds.
Parasite immunology38(6)333-9(2016 06)
Jacobs JR,Sommers KN,Zajac AM,Notter DR,Bowdridge SA

Prolongation of sheep corneal allograft survival by transfer of the gene encoding ovine IL-12-p40 but not IL-4 to donor corneal endothelium.
Journal of immunology (Baltimore, Md. : 1950)175(4)2219-26(2005 Aug)
Klebe S,Coster DJ,Sykes PJ,Swinburne S,Hallsworth P,Scheerlinck JP,Krishnan R,Williams KA

Generation and characterization of ovine dendritic cells derived from peripheral blood monocytes.
Immunology107(3)366-72(2002 Nov)
Chan SS,McConnell I,Blacklaws BA

The expression and biologic effects of ovine interleukin-4 on T and B cell proliferation.
Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research20(4)419-25(2000 Apr)
Chaplin PJ,Casey G,De Rose R,Buchan G,Wood PR,Scheerlinck JP

Cloning and sequencing an ovine interleukin-4-encoding cDNA.
Gene124(2)291-3(1993 Feb)
Seow HF,Rothel JS,Wood PR