crim1 cDNA ORF clone,

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gRNAs

The following crim1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the crim1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFl16079	XM_030719611.1 Latest version!	Archocentrus centrarchus cysteine rich transmembrane BMP regulator 1 (crim1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

crim1 ( XM_030719611.1 ) cDNA ORF clone, -> XP_030575471.1 Archocentrus centrarchus cysteine rich transmembrane BMP regulator 1 (crim1), mRNA.

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CloneID	OFl16079
Clone ID Related Accession (Same CDS sequence)	XM_030719611.1
Accession Version	XM_030719611.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2814bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-05
Organism	Archocentrus centrarchus(flier cichlid)
Product	cysteine-rich motor neuron 1 protein
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_044367.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Archocentrus centrarchus Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCAGCA AATGCTCCGT GGTGTTTTTG AGTGTCCTCT TTCTTGTGCG TGCGCTCTCG 
GCTCACGAGT GCACGCCGTG CAACCTCCGG ACTTGTCCCT TCAAATCCCC GCTTGCTTGC 
GAAGGTAATC GGACGCCGGC CAAGGATCCG TGCGGATGCT GTGATCAGTG CACCCGGCTG 
GAGTGGGAGC CCTGCGGCGG CCAGGACTGG ACGCACGGCT ACTGCGCCCT GGGACTGACC 
TGCGCCTCTG TAAACAAAAC CGGAGCTGCC ACTATCCCTG AAATTGGAGT ATGTAAAGTA 
CTCCCTGACT ATCCAGACGC AGGTCTCGAG GATGAGCGCT GTCCACTGAT GACAGGCTGC 
GACAAAACAG GAGGTCAGTG TGTGTGTGAT GCCCGTCACT CCTGCTTGGG CTCGTTTACC 
TACCCAGACC AAGAAACCTG CATGAAGCCC ACTAAGTCAG ATGGTCGGAG GCACGAGCAT 
CGGGAAAAGC ACAGAGAGAA GTATGTGGGA CCATCCAACC CAGCTTGTAT GTTCTCTGGC 
TGTAACCTGA CCACTGAGGG CTGTGTGTGT GAGTCTCAGA GCTGCCACCA CCACTTCGCC 
TACATCAACC GCAGCCAGTG CCAGGAAGCT GCAGAGGAGA TGAGGTGTGC TGGGGTGAAA 
TGCCCCACTC TGCAGGTGCC CTCTTGCCCC GAGGGTTCAG TCCTGACCAA GAGCTACAAG 
CCTCCTGCTG GATGCTGCCC CTCCGTACCA CCCCAGTGCA CTTGCAACTT CCGTGCCTGC 
CACAAGCCCC AGTGCCCGAG CGGACAGCAC CCTGTTTCAC TTGGGCAAGC CAGCGGTCAA 
CCAGGAGACT GCTGTGATGT CTTTGTGTGT CAGAAAGTCT CTCCCAAGTG TGTTCACAAT 
GGGAAAGAGT ACTCTGAGGG AGAAGTGTAC CGCATGGACC CGTGCTGGCT GTGTCAGTGC 
CGGGGAGGAA TCTCCTTCTG CTCCAAGGCA GAGTGTGCTG AGCTGGACTG TGAAAACTTC 
TACATTCCCG AGGGAGAGTG TTGCCCTGTC TGCATAGATG TGGAGTTACT GTCAATGGAT 
AGTGCTGAGG CCAGCTGCTG GGTGAATAAT AAGCTGCGAG CCCACGAGGA GCAGTGGAAA 
GAGGACGACT GCACCTTCTG CCAGTGTGTG GATGGAGAAC CGCACTGCAC GGCCATGGCC 
TGCAAACAGA GTTGCCAAAA CCCCGTGAAG ATCCCCGGAG AATGCTGTCC CTTCTGTGAA 
GAGCCTTCCT ATGAAACAGT AAGCCCCTTG CTGTGTCCTC CCCTGGAAAA CTGCTCCCTG 
TCAGTGAATG ACTGCCCCTA TGGCTTCCAA CAGGACAAAA ATGGATGTCT TCTTTGCCAG 
TGTCTCAACA ACGACTCCTG CCCGGACCTG GGAACGTACT GTTCCCTGCA GTGTCCAAGG 
GGATATGAGA GAGATGATTT TGGATGTGAG GTGTGTGAGT GCACTGTCCC TATTCCAAAG 
TGCCGACCTC TGACCTGCAC TAAGACCTGC CCCTATGGAT ATGTCAGGAA CAAGCATGGC 
TGCGAGATGT GTCGCTGTGT CAAGTGTCCT CCCTTCACCT GCGACAAGCA CTGCATTAAT 
GGATATCAGC AGAACAGGAA GGGCTGCTCC ATCTGCATGT GTAAAGAAAG CGGCCATGTC 
CCAAGCACCA CTGCCCCGGC ACCGATGCCC AACTATTGCC TTACGTCCAA TGGGCAGAAA 
TACGAGGAAG GTGAGGGCTG GCACGATGGC TGTCGCGACT GCTATTGTCA CTCCGGACGG 
GAGATGTGTG TGCTCATATC CTGCCCAGTG CCCAGCTGTC TTCATCCTGT TGTCAAGCCT 
AACCAGTGCT GTCCTACCTG TGAGGATGAC TCTGGCAGCG GTCAGCCAGA GGGGATGGAC 
ATGGTGGTGT GCAGAGCACC TGGGGGAGAG TTTTATGTGG AGGGGGAAAC CTGGAACCTA 
GACGAATGCA CACGCTGCAC CTGCAGGAAG GGACGTGTCC TGTGTGACAC CGAAGTGTGT 
CCCCCAGCTC TTTGTCAAGC ACCAATCAGG AACAAGGACA CCTGCTGCCA TGTGTGCCCA 
GAAGAAACAC CTTTGCTGCC AGTGAACAAC AGCCAGCAGG AGTACTGCAT AACCAGCGAT 
GGAGACATGC TGCTAGCTGG AGACTCCTGG AAAGCCAATG CCTGCACCAG CTGCACCTGC 
AACAACGGCA CTATTCAGTG TTTCTCTCAG CGCTGTCCAG CTGCCAACTG CCGGGTGCCT 
GTTTTACGCA AGGGCCAGTG CTGCCCACAC TGTCTCGAAA TGACAACATC AGTGCCAGTG 
ATGGTGTCTA CAAACGCCGC CAAGACGAGA GTCACCACGA CAATGGAGAG CGCAGAGTGG 
CTCACCACTG TCACTGTACC GCCTGTCATT GCTGATACAG ATGAGCCACA TTTGGGTGAG 
AATTACCCCA GCCAAACAGA GATGGCTCTC ATTTACCAAT CGGCTGCCTG GATCCTGGCG 
GGTCTCCTCT TAGCCATCGT GATCTTTCTT ATTGTGGCGT TACTCATCAA CAAGAAGAAG 
AAGTGGGTGC AGATGTCTTG CTACAGTGCA CCAAAGAAGG CAGTGATCCT GAAGAAGCAT 
GTCAACAAGA ATTCAGTGGT CTACATGGAG CCTTCGAAAG AGAACAAATT TCACAATGTG 
AAGAACGACT GCGGCATCAT TCACTTCTCT CCAGAAATGA GCTCCCCCTG TACAGAAAAG 
ATGACCATCC CCCGGGCTAA GCTGAGCATG GGCAATGACT GGACGCCATG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030575471.1
CDS	398..3211
Translation	MVSKCSVVFLSVLFLVRALSAHECTPCNLRTCPFKSPLACEGNRTPAKDPCGCCDQCTRLEWEPCGGQDWTHGYCALGLTCASVNKTGAATIPEIGVCKVLPDYPDAGLEDERCPLMTGCDKTGGQCVCDARHSCLGSFTYPDQETCMKPTKSDGRRHEHREKHREKYVGPSNPACMFSGCNLTTEGCVCESQSCHHHFAYINRSQCQEAAEEMRCAGVKCPTLQVPSCPEGSVLTKSYKPPAGCCPSVPPQCTCNFRACHKPQCPSGQHPVSLGQASGQPGDCCDVFVCQKVSPKCVHNGKEYSEGEVYRMDPCWLCQCRGGISFCSKAECAELDCENFYIPEGECCPVCIDVELLSMDSAEASCWVNNKLRAHEEQWKEDDCTFCQCVDGEPHCTAMACKQSCQNPVKIPGECCPFCEEPSYETVSPLLCPPLENCSLSVNDCPYGFQQDKNGCLLCQCLNNDSCPDLGTYCSLQCPRGYERDDFGCEVCECTVPIPKCRPLTCTKTCPYGYVRNKHGCEMCRCVKCPPFTCDKHCINGYQQNRKGCSICMCKESGHVPSTTAPAPMPNYCLTSNGQKYEEGEGWHDGCRDCYCHSGREMCVLISCPVPSCLHPVVKPNQCCPTCEDDSGSGQPEGMDMVVCRAPGGEFYVEGETWNLDECTRCTCRKGRVLCDTEVCPPALCQAPIRNKDTCCHVCPEETPLLPVNNSQQEYCITSDGDMLLAGDSWKANACTSCTCNNGTIQCFSQRCPAANCRVPVLRKGQCCPHCLEMTTSVPVMVSTNAAKTRVTTTMESAEWLTTVTVPPVIADTDEPHLGENYPSQTEMALIYQSAAWILAGLLLAIVIFLIVALLINKKKKWVQMSCYSAPKKAVILKKHVNKNSVVYMEPSKENKFHNVKNDCGIIHFSPEMSSPCTEKMTIPRAKLSMGNDWTPC

Target ORF information:

RefSeq Version	XM_030719611.1
Organism	Archocentrus centrarchus(flier cichlid)
Definition	Archocentrus centrarchus cysteine rich transmembrane BMP regulator 1 (crim1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030719611.1

ORF Insert Sequence:

ATGGTCAGCA AATGCTCCGT GGTGTTTTTG AGTGTCCTCT TTCTTGTGCG TGCGCTCTCG 
GCTCACGAGT GCACGCCGTG CAACCTCCGG ACTTGTCCCT TCAAATCCCC GCTTGCTTGC 
GAAGGTAATC GGACGCCGGC CAAGGATCCG TGCGGATGCT GTGATCAGTG CACCCGGCTG 
GAGTGGGAGC CCTGCGGCGG CCAGGACTGG ACGCACGGCT ACTGCGCCCT GGGACTGACC 
TGCGCCTCTG TAAACAAAAC CGGAGCTGCC ACTATCCCTG AAATTGGAGT ATGTAAAGTA 
CTCCCTGACT ATCCAGACGC AGGTCTCGAG GATGAGCGCT GTCCACTGAT GACAGGCTGC 
GACAAAACAG GAGGTCAGTG TGTGTGTGAT GCCCGTCACT CCTGCTTGGG CTCGTTTACC 
TACCCAGACC AAGAAACCTG CATGAAGCCC ACTAAGTCAG ATGGTCGGAG GCACGAGCAT 
CGGGAAAAGC ACAGAGAGAA GTATGTGGGA CCATCCAACC CAGCTTGTAT GTTCTCTGGC 
TGTAACCTGA CCACTGAGGG CTGTGTGTGT GAGTCTCAGA GCTGCCACCA CCACTTCGCC 
TACATCAACC GCAGCCAGTG CCAGGAAGCT GCAGAGGAGA TGAGGTGTGC TGGGGTGAAA 
TGCCCCACTC TGCAGGTGCC CTCTTGCCCC GAGGGTTCAG TCCTGACCAA GAGCTACAAG 
CCTCCTGCTG GATGCTGCCC CTCCGTACCA CCCCAGTGCA CTTGCAACTT CCGTGCCTGC 
CACAAGCCCC AGTGCCCGAG CGGACAGCAC CCTGTTTCAC TTGGGCAAGC CAGCGGTCAA 
CCAGGAGACT GCTGTGATGT CTTTGTGTGT CAGAAAGTCT CTCCCAAGTG TGTTCACAAT 
GGGAAAGAGT ACTCTGAGGG AGAAGTGTAC CGCATGGACC CGTGCTGGCT GTGTCAGTGC 
CGGGGAGGAA TCTCCTTCTG CTCCAAGGCA GAGTGTGCTG AGCTGGACTG TGAAAACTTC 
TACATTCCCG AGGGAGAGTG TTGCCCTGTC TGCATAGATG TGGAGTTACT GTCAATGGAT 
AGTGCTGAGG CCAGCTGCTG GGTGAATAAT AAGCTGCGAG CCCACGAGGA GCAGTGGAAA 
GAGGACGACT GCACCTTCTG CCAGTGTGTG GATGGAGAAC CGCACTGCAC GGCCATGGCC 
TGCAAACAGA GTTGCCAAAA CCCCGTGAAG ATCCCCGGAG AATGCTGTCC CTTCTGTGAA 
GAGCCTTCCT ATGAAACAGT AAGCCCCTTG CTGTGTCCTC CCCTGGAAAA CTGCTCCCTG 
TCAGTGAATG ACTGCCCCTA TGGCTTCCAA CAGGACAAAA ATGGATGTCT TCTTTGCCAG 
TGTCTCAACA ACGACTCCTG CCCGGACCTG GGAACGTACT GTTCCCTGCA GTGTCCAAGG 
GGATATGAGA GAGATGATTT TGGATGTGAG GTGTGTGAGT GCACTGTCCC TATTCCAAAG 
TGCCGACCTC TGACCTGCAC TAAGACCTGC CCCTATGGAT ATGTCAGGAA CAAGCATGGC 
TGCGAGATGT GTCGCTGTGT CAAGTGTCCT CCCTTCACCT GCGACAAGCA CTGCATTAAT 
GGATATCAGC AGAACAGGAA GGGCTGCTCC ATCTGCATGT GTAAAGAAAG CGGCCATGTC 
CCAAGCACCA CTGCCCCGGC ACCGATGCCC AACTATTGCC TTACGTCCAA TGGGCAGAAA 
TACGAGGAAG GTGAGGGCTG GCACGATGGC TGTCGCGACT GCTATTGTCA CTCCGGACGG 
GAGATGTGTG TGCTCATATC CTGCCCAGTG CCCAGCTGTC TTCATCCTGT TGTCAAGCCT 
AACCAGTGCT GTCCTACCTG TGAGGATGAC TCTGGCAGCG GTCAGCCAGA GGGGATGGAC 
ATGGTGGTGT GCAGAGCACC TGGGGGAGAG TTTTATGTGG AGGGGGAAAC CTGGAACCTA 
GACGAATGCA CACGCTGCAC CTGCAGGAAG GGACGTGTCC TGTGTGACAC CGAAGTGTGT 
CCCCCAGCTC TTTGTCAAGC ACCAATCAGG AACAAGGACA CCTGCTGCCA TGTGTGCCCA 
GAAGAAACAC CTTTGCTGCC AGTGAACAAC AGCCAGCAGG AGTACTGCAT AACCAGCGAT 
GGAGACATGC TGCTAGCTGG AGACTCCTGG AAAGCCAATG CCTGCACCAG CTGCACCTGC 
AACAACGGCA CTATTCAGTG TTTCTCTCAG CGCTGTCCAG CTGCCAACTG CCGGGTGCCT 
GTTTTACGCA AGGGCCAGTG CTGCCCACAC TGTCTCGAAA TGACAACATC AGTGCCAGTG 
ATGGTGTCTA CAAACGCCGC CAAGACGAGA GTCACCACGA CAATGGAGAG CGCAGAGTGG 
CTCACCACTG TCACTGTACC GCCTGTCATT GCTGATACAG ATGAGCCACA TTTGGGTGAG 
AATTACCCCA GCCAAACAGA GATGGCTCTC ATTTACCAAT CGGCTGCCTG GATCCTGGCG 
GGTCTCCTCT TAGCCATCGT GATCTTTCTT ATTGTGGCGT TACTCATCAA CAAGAAGAAG 
AAGTGGGTGC AGATGTCTTG CTACAGTGCA CCAAAGAAGG CAGTGATCCT GAAGAAGCAT 
GTCAACAAGA ATTCAGTGGT CTACATGGAG CCTTCGAAAG AGAACAAATT TCACAATGTG 
AAGAACGACT GCGGCATCAT TCACTTCTCT CCAGAAATGA GCTCCCCCTG TACAGAAAAG 
ATGACCATCC CCCGGGCTAA GCTGAGCATG GGCAATGACT GGACGCCATG CTAA