twsg1 cDNA ORF clone, Oryzias latipes(Japanese medaka)

Gene
Clones
gRNAs

Summary

Gene Symbol	twsg1
Entrez Gene ID	101172607
Full Name	twisted gastrulation BMP signaling modulator 1
Gene Type	protein-coding
Organism	Oryzias latipes(Japanese medaka)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004078614.3	XP_004078662.1	twisted gastrulation protein homolog 1
XM_004078614.4	XP_004078662.1	twisted gastrulation protein homolog 1

Pathways from BioSystems
Gene Ontology

The following twsg1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the twsg1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OJa12866	XM_004078614.4 Latest version!	Oryzias latipes twisted gastrulation BMP signaling modulator 1 (twsg1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
View Old Accession Versions >>
OJa12866	XM_004078614.3	Oryzias latipes twisted gastrulation BMP signaling modulator 1 (twsg1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

twsg1 ( XM_004078614.3 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_004078662.1 Oryzias latipes twisted gastrulation BMP signaling modulator 1 (twsg1), mRNA.

Price & Availability↑

CloneID	OJa12866
Clone ID Related Accession (Same CDS sequence)	XM_004078614.3 , XM_004078614.4
Accession Version	XM_004078614.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 669bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-06
Organism	Oryzias latipes(Japanese medaka)
Product	twisted gastrulation protein homolog 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019875.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 7, 2017 this sequence version replaced XM_004078614.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryzias latipes Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGCCTG CTCAGCTGAT CCTCCCCGCC ACTGCTGCTC TGCTCTTCGT CCTGTCAGGA 
CTGTCTCTGA CCTCTGGCTG CAACAAAGCC CTCTGCGCCA GCGACGTCAG CAAATGTCTC 
ATCCAGGAGC TTTGCCAGTG CCGCCCCTCC GATGACAACT GCTCCTGCTG CAAAGACTGC 
ATGCTTTGTC TGGGGAACCT CTGGGAGGAG TGCTGCGACT GCGTGGGAAT GTGTAACCCC 
AAAAACTACA ACGACTCTCC TGCGACATCC AAGAGCACGG TGGAGGAGCT GCACCGCCCC 
ATCCCCTCGC TGTTCCGCGC TCTGACGGAG GGTGACGCTC CCATCAACAT GATGGTGGTG 
TCGTTCCCCG TCGCCGAGGA GCTCTCACAT CACGAGAACT TGGTGTCTTT CCTGGAGTCG 
CTCGACAGCC AGGATCCCAA CGTGTCAAAG GCCAGGAACA GTCTCCATCC CGGCTACGAC 
AATCAAGAAA ACATGTGCAC GGTGGTCTAC TTTGACGACT GCGTGTCCAT CCATCAGTGT 
AAACAGTATT GTGAGTCGAT GGGAGGGTCG AAGTACCGCT GGTTTCACAA CGCCTGCTGC 
CAGTGCATCG GGCCGGAGTG CGTGGACTAC GGCAGCAAGG CTGTGAAGTG CTTGAACTGT 
CTCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004078662.1
CDS	988..1656
Translation	MRPAQLILPATAALLFVLSGLSLTSGCNKALCASDVSKCLIQELCQCRPSDDNCSCCKDCMLCLGNLWEECCDCVGMCNPKNYNDSPATSKSTVEELHRPIPSLFRALTEGDAPINMMVVSFPVAEELSHHENLVSFLESLDSQDPNVSKARNSLHPGYDNQENMCTVVYFDDCVSIHQCKQYCESMGGSKYRWFHNACCQCIGPECVDYGSKAVKCLNCLF

Target ORF information:

RefSeq Version	XM_004078614.3
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes twisted gastrulation BMP signaling modulator 1 (twsg1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004078614.3

ORF Insert Sequence:

ATGAGGCCTG CTCAGCTGAT CCTCCCCGCC ACTGCTGCTC TGCTCTTCGT CCTGTCAGGA 
CTGTCTCTGA CCTCTGGCTG CAACAAAGCC CTCTGCGCCA GCGACGTCAG CAAATGTCTC 
ATCCAGGAGC TTTGCCAGTG CCGCCCCTCC GATGACAACT GCTCCTGCTG CAAAGACTGC 
ATGCTTTGTC TGGGGAACCT CTGGGAGGAG TGCTGCGACT GCGTGGGAAT GTGTAACCCC 
AAAAACTACA ACGACTCTCC TGCGACATCC AAGAGCACGG TGGAGGAGCT GCACCGCCCC 
ATCCCCTCGC TGTTCCGCGC TCTGACGGAG GGTGACGCTC CCATCAACAT GATGGTGGTG 
TCGTTCCCCG TCGCCGAGGA GCTCTCACAT CACGAGAACT TGGTGTCTTT CCTGGAGTCG 
CTCGACAGCC AGGATCCCAA CGTGTCAAAG GCCAGGAACA GTCTCCATCC CGGCTACGAC 
AATCAAGAAA ACATGTGCAC GGTGGTCTAC TTTGACGACT GCGTGTCCAT CCATCAGTGT 
AAACAGTATT GTGAGTCGAT GGGAGGGTCG AAGTACCGCT GGTTTCACAA CGCCTGCTGC 
CAGTGCATCG GGCCGGAGTG CGTGGACTAC GGCAGCAAGG CTGTGAAGTG CTTGAACTGT 
CTCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

twsg1 ( XM_004078614.4 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_004078662.1 Oryzias latipes twisted gastrulation BMP signaling modulator 1 (twsg1), mRNA.

Price & Availability↑

CloneID	OJa12866
Clone ID Related Accession (Same CDS sequence)	XM_004078614.3 , XM_004078614.4
Accession Version	XM_004078614.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 669bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-02-14
Organism	Oryzias latipes(Japanese medaka)
Product	twisted gastrulation protein homolog 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019875.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 15, 2018 this sequence version replaced XM_004078614.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGCCTG CTCAGCTGAT CCTCCCCGCC ACTGCTGCTC TGCTCTTCGT CCTGTCAGGA 
CTGTCTCTGA CCTCTGGCTG CAACAAAGCC CTCTGCGCCA GCGACGTCAG CAAATGTCTC 
ATCCAGGAGC TTTGCCAGTG CCGCCCCTCC GATGACAACT GCTCCTGCTG CAAAGACTGC 
ATGCTTTGTC TGGGGAACCT CTGGGAGGAG TGCTGCGACT GCGTGGGAAT GTGTAACCCC 
AAAAACTACA ACGACTCTCC TGCGACATCC AAGAGCACGG TGGAGGAGCT GCACCGCCCC 
ATCCCCTCGC TGTTCCGCGC TCTGACGGAG GGTGACGCTC CCATCAACAT GATGGTGGTG 
TCGTTCCCCG TCGCCGAGGA GCTCTCACAT CACGAGAACT TGGTGTCTTT CCTGGAGTCG 
CTCGACAGCC AGGATCCCAA CGTGTCAAAG GCCAGGAACA GTCTCCATCC CGGCTACGAC 
AATCAAGAAA ACATGTGCAC GGTGGTCTAC TTTGACGACT GCGTGTCCAT CCATCAGTGT 
AAACAGTATT GTGAGTCGAT GGGAGGGTCG AAGTACCGCT GGTTTCACAA CGCCTGCTGC 
CAGTGCATCG GGCCGGAGTG CGTGGACTAC GGCAGCAAGG CTGTGAAGTG CTTGAACTGT 
CTCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004078662.1
CDS	992..1660
Translation	MRPAQLILPATAALLFVLSGLSLTSGCNKALCASDVSKCLIQELCQCRPSDDNCSCCKDCMLCLGNLWEECCDCVGMCNPKNYNDSPATSKSTVEELHRPIPSLFRALTEGDAPINMMVVSFPVAEELSHHENLVSFLESLDSQDPNVSKARNSLHPGYDNQENMCTVVYFDDCVSIHQCKQYCESMGGSKYRWFHNACCQCIGPECVDYGSKAVKCLNCLF

Target ORF information:

RefSeq Version	XM_004078614.4
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes twisted gastrulation BMP signaling modulator 1 (twsg1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004078614.4

ORF Insert Sequence:

ATGAGGCCTG CTCAGCTGAT CCTCCCCGCC ACTGCTGCTC TGCTCTTCGT CCTGTCAGGA 
CTGTCTCTGA CCTCTGGCTG CAACAAAGCC CTCTGCGCCA GCGACGTCAG CAAATGTCTC 
ATCCAGGAGC TTTGCCAGTG CCGCCCCTCC GATGACAACT GCTCCTGCTG CAAAGACTGC 
ATGCTTTGTC TGGGGAACCT CTGGGAGGAG TGCTGCGACT GCGTGGGAAT GTGTAACCCC 
AAAAACTACA ACGACTCTCC TGCGACATCC AAGAGCACGG TGGAGGAGCT GCACCGCCCC 
ATCCCCTCGC TGTTCCGCGC TCTGACGGAG GGTGACGCTC CCATCAACAT GATGGTGGTG 
TCGTTCCCCG TCGCCGAGGA GCTCTCACAT CACGAGAACT TGGTGTCTTT CCTGGAGTCG 
CTCGACAGCC AGGATCCCAA CGTGTCAAAG GCCAGGAACA GTCTCCATCC CGGCTACGAC 
AATCAAGAAA ACATGTGCAC GGTGGTCTAC TTTGACGACT GCGTGTCCAT CCATCAGTGT 
AAACAGTATT GTGAGTCGAT GGGAGGGTCG AAGTACCGCT GGTTTCACAA CGCCTGCTGC 
CAGTGCATCG GGCCGGAGTG CGTGGACTAC GGCAGCAAGG CTGTGAAGTG CTTGAACTGT 
CTCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.