phka1 cDNA ORF clone, Oryzias latipes(Japanese medaka)

Gene
Clones
gRNAs

Summary

Gene Symbol	phka1
Entrez Gene ID	101170056
Full Name	phosphorylase kinase regulatory subunit alpha 1
Gene Type	protein-coding
Organism	Oryzias latipes(Japanese medaka)

mRNA and Protein(s)

mRNA	Protein	Name
XM_020711742.2	XP_020567401.1	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X3
XM_011487671.2	XP_011485973.1	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X1
XM_020711742.1	XP_020567401.1	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X3
XM_011487671.3	XP_011485973.1	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X1
XM_011487672.2	XP_011485974.1	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X2
XM_011487672.3	XP_011485974.1	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X2

Pathways from BioSystems
Gene Ontology

The following phka1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the phka1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OJa74016	XM_020711742.2 Latest version!	Oryzias latipes phosphorylase kinase regulatory subunit alpha 1 (phka1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OJa32967	XM_011487671.3 Latest version!	Oryzias latipes phosphorylase kinase regulatory subunit alpha 1 (phka1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OJa32968	XM_011487672.3 Latest version!	Oryzias latipes phosphorylase kinase regulatory subunit alpha 1 (phka1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
View Old Accession Versions >>
OJa74016	XM_020711742.1	Oryzias latipes phosphorylase kinase regulatory subunit alpha 1 (phka1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OJa32967	XM_011487671.2	Oryzias latipes phosphorylase kinase regulatory subunit alpha 1 (phka1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OJa32968	XM_011487672.2	Oryzias latipes phosphorylase kinase regulatory subunit alpha 1 (phka1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

phka1 ( XM_020711742.2 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_020567401.1 Oryzias latipes phosphorylase kinase regulatory subunit alpha 1 (phka1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OJa74016
Clone ID Related Accession (Same CDS sequence)	XM_020711742.2 , XM_020711742.1
Accession Version	XM_020711742.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3138bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-02-14
Organism	Oryzias latipes(Japanese medaka)
Product	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019876.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 15, 2018 this sequence version replaced XM_020711742.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGCC GAAGCAACTC CGGCGTGAAG CTGGACAACT ATGCCCGGAT CGTCCAGCAG 
ACCATCCTGC AGCACCAGGA TCCAGTAACG GGTCTCCTTC CGGGCAGCTT GGAGCAGCCA 
GATGCATGGG TGAGGGACAA CGTCTACAGC ATCGTCTCAG TGTGGGCCCT CAGCCTGGCC 
TACAGGAAAA ATGCCGACCG GGACGAGGAC AAGGCCAAGG CCTACGAGCT GGAGCAGAGT 
GTGGTGAAGC TCATGAGAGG TGTCCTCCAG TGCATTATGA GACAGCTAGA CAAAGTGGAG 
AAGTTCAAAT ACAGCCGAAG TACCAAGGAC TCACTTCACG CCAAGTACAA CCCACGCACC 
TGTGCTACAG TGGTCGGGGA CCACCAGTGG GGTCACCTGC AGGTGGACGC AACCTCGCTC 
TTCCTGCTCT TCCTCGCTCA GATGACGGCA TCCGGTCTGC ACATCATCTA CACGCAGGAT 
GAGGTGGACG TCGTTCAGAA CCTCATGTTC TACATCGAGG CAGCTTACAA AGTGGCTGAT 
TATGGGATGT GGGAGAGGGG GGACAAAACC AACCAGGGCA TCACAGAGAT CAATGCCAGC 
TCCATCGGCA TGGCCAAGGC GGCTCTGGAG GCCCTGGACG AACTCAACCT GTTTGGAGCT 
AAGGGAGGAC CGGGATCTGT GGTCCACGCT CTGGCTGATG ACATCCAGCA CTGCCAGTCC 
ATCCTCACAT CCATGTTACC CAAAGCTTCC ATCTCTAAAG AGGTGGATGC CGGAGTTTTG 
GCTATTCTTA CCTACCCCGC CTTCGCCGTG GAGGACATGA GCATCGTCAC CATGACCAAG 
GAGGAGATCA TCTCCAAGCT GCAGGGTCGG TACGGCTGTT GCAGGTTTCT CAGGGACGGA 
CATAAAACAC CCAAAGAGGA TCCCAACCGC CTGTACTACG AGTCTGCAGA GCTGAAGCTG 
TTCGAGAACA TCGAGTGTGA GTGGCCTCTC TTCTGGACGT ACCTCATTCT GGACGGCATA 
TTCAGCAACA GTCATGAACA GGTGCAGGAG TACCAGGAGG CGCTGGAGGG CATCCTCATC 
AAGCAGAAGG ATGGCATACG ACTGTTTCCA GAGCTCTACA GCGTTCCTCC AGATAAGGTG 
GAAGAGGAAT ACATGAACCC CCACACAGTG GAGAGGATCC CGATGGGGAA ATGTCCCCTG 
AAATGGGGCC AGTCCCTTTA TGTTCTGGGG AGACTCTTAT CCGAGGGGTT TCTTGCTCCT 
GGTGAGATTG ACCCTCTTAA CCGCCGTTTC TCCACCGTGC CCAAGCCGGA CGTGGTCGTT 
CAGGTTTCCA TTTTGGCTGA AACAGATGAG ATTAAGGAGC TTTTGCTGAA GAACGGCTTC 
GAGGTGGAAA CCATCGCAGA CATCCACCCC TTTCATGTGC AGCCCTCCAG AGTCCTCAGC 
CACATCTATG CTAGGCTCGG ACGTAACCCA AGGCTGGGCC TGACGGGACG GCCCTACAGG 
AGAATCGGAG TTCTGGGAAC CTCAAAGTTC TACATCATCA GAAACACCAT CTTCACATTC 
ACTCCACAGT TCATCGACCA CCAGCAGTTC TACCTGGCTC TGGACAACAA AATGATCGTA 
GAGATGTTGA GGACAGAAAT CGCCTACCTG TCCTCCAGGT GGAGGATAAC CGGACGGCCG 
ACGGTCACCT TCCCCGTCTC TCAAACCATG CTGACTGAAG ACCGCACAGA TCTGGACCCG 
GCTGTCCTGG CGACGCTGAA GAAGCTACAG GATGGGTATT TTGGAGGAGC CAGGATCCAG 
ACGGGAAAGC TGTCAGAGTT CCTGACCACC TCCTGCTGCG CTCACCTGAG CTTCCTGGAT 
GGTAAGCGCT TCGGCAGCAT GGGTTCCCAC GCCGAAGAGT ATGACCATGA TGATGCCAGC 
GATGGATATG TGCATGAGTT GCATTGTGAT GATGAGGCAG ACGACCTTGC TCAGTACTTG 
GATCACCTCC TGGCTCACTC TGCTTCTAAG AAGCAGAAGT CCAGCGCTGA AGGTGTGGGC 
CGATTCAAAG CTGTTGCTAC AAGAACCAGG GAGATGGTCT CTTTGATGAA CAAGGCTCAG 
GATCTTAACA TCCACAACAT TGGCATGTAC CTTCCCAACA AGCTGTTTCG TACTCGTCAT 
CCATCGCTCA ATCTGAACCT TCCAGACAGT ACTGTCCACG AAGAAGCTCA GGCACCAGAG 
GTGTCCATCA CCACAGAAAG TAGCCTCCCC AGAGATGCCA GCGGCGCCAT CGACTACAGT 
GCTTTGGTCC AGCTGCTGAA AGACAGTGGG AACATCCAGG ACCAGGCAGA TATTCTCTAC 
ATCCTCTTCA AAGACAAGGG GATGGACTGG GACACACACC TTCATGGGAG GGGCTCCACT 
GTCCGGTCTC TGCTCAGCAA CCTTTACGAG AAGGCTGGTG ACCTCAAACA CTGGGGCCTG 
ATCCGGATGA TCTCTGGGAT GCTGAAGAAG AAGGTGGAAG AGCTCGACTC GGCCTGCTCT 
GACCTGCTGG CTCACCAGAA GCACCTGACA GTAGGTCTGC CTCCAGAACC CAGGGAGAAA 
ACCATCACGG CGCCCATCCC TCCAGACCAG CTGGCTGCCC TCATCGATGA GGCCAGCGAT 
GGCAACATCA GTGTTGCCAT CCTCACACAG GAGATCATGG TGTTCCTGGC CATGAGCATC 
CGCACGCAGC CCAGTCTGTT CAGCGAGATG TTCAGGCTCC GGATCGGCCT CATCATTCAA 
GTCATGGCCA CAGAACTGGC TCAGTCCCTC AACTGCTCTG GTGACGAGGC CACAGAGAGT 
CTGATGAGTC TGAGTCCGTC AGAGCTGAAG AACCTGCTCC ACCACATCCT CAGTGGGAAA 
GAGTTTGGCG TGGAGCGCAG CGTCAGAGAG GGAGATGATG GCATCAGTCC TGCCATCTCC 
ATCCACCACC TGGGGAACGT TGGCGCCACC AAGAGTGAGA GAGCGGGCAT CAGCAAGCTG 
AAGAGCGACA TAAAGATGCT TGAGCACACG ATGTCTCTGT CTGTGAGTAT GATAAAAAAT 
GATCATCTTA TTGGTGCTAA TTCACCTGAT GGAAGGGAAG GAGAGCTTGA GATGCATGGG 
GCAAATCATG ACGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020567401.1
CDS	192..3329
Translation	MRSRSNSGVKLDNYARIVQQTILQHQDPVTGLLPGSLEQPDAWVRDNVYSIVSVWALSLAYRKNADRDEDKAKAYELEQSVVKLMRGVLQCIMRQLDKVEKFKYSRSTKDSLHAKYNPRTCATVVGDHQWGHLQVDATSLFLLFLAQMTASGLHIIYTQDEVDVVQNLMFYIEAAYKVADYGMWERGDKTNQGITEINASSIGMAKAALEALDELNLFGAKGGPGSVVHALADDIQHCQSILTSMLPKASISKEVDAGVLAILTYPAFAVEDMSIVTMTKEEIISKLQGRYGCCRFLRDGHKTPKEDPNRLYYESAELKLFENIECEWPLFWTYLILDGIFSNSHEQVQEYQEALEGILIKQKDGIRLFPELYSVPPDKVEEEYMNPHTVERIPMGKCPLKWGQSLYVLGRLLSEGFLAPGEIDPLNRRFSTVPKPDVVVQVSILAETDEIKELLLKNGFEVETIADIHPFHVQPSRVLSHIYARLGRNPRLGLTGRPYRRIGVLGTSKFYIIRNTIFTFTPQFIDHQQFYLALDNKMIVEMLRTEIAYLSSRWRITGRPTVTFPVSQTMLTEDRTDLDPAVLATLKKLQDGYFGGARIQTGKLSEFLTTSCCAHLSFLDGKRFGSMGSHAEEYDHDDASDGYVHELHCDDEADDLAQYLDHLLAHSASKKQKSSAEGVGRFKAVATRTREMVSLMNKAQDLNIHNIGMYLPNKLFRTRHPSLNLNLPDSTVHEEAQAPEVSITTESSLPRDASGAIDYSALVQLLKDSGNIQDQADILYILFKDKGMDWDTHLHGRGSTVRSLLSNLYEKAGDLKHWGLIRMISGMLKKKVEELDSACSDLLAHQKHLTVGLPPEPREKTITAPIPPDQLAALIDEASDGNISVAILTQEIMVFLAMSIRTQPSLFSEMFRLRIGLIIQVMATELAQSLNCSGDEATESLMSLSPSELKNLLHHILSGKEFGVERSVREGDDGISPAISIHHLGNVGATKSERAGISKLKSDIKMLEHTMSLSVSMIKNDHLIGANSPDGREGELEMHGANHDA

Target ORF information:

RefSeq Version	XM_020711742.2
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes phosphorylase kinase regulatory subunit alpha 1 (phka1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020711742.2

ORF Insert Sequence:

ATGAGGAGCC GAAGCAACTC CGGCGTGAAG CTGGACAACT ATGCCCGGAT CGTCCAGCAG 
ACCATCCTGC AGCACCAGGA TCCAGTAACG GGTCTCCTTC CGGGCAGCTT GGAGCAGCCA 
GATGCATGGG TGAGGGACAA CGTCTACAGC ATCGTCTCAG TGTGGGCCCT CAGCCTGGCC 
TACAGGAAAA ATGCCGACCG GGACGAGGAC AAGGCCAAGG CCTACGAGCT GGAGCAGAGT 
GTGGTGAAGC TCATGAGAGG TGTCCTCCAG TGCATTATGA GACAGCTAGA CAAAGTGGAG 
AAGTTCAAAT ACAGCCGAAG TACCAAGGAC TCACTTCACG CCAAGTACAA CCCACGCACC 
TGTGCTACAG TGGTCGGGGA CCACCAGTGG GGTCACCTGC AGGTGGACGC AACCTCGCTC 
TTCCTGCTCT TCCTCGCTCA GATGACGGCA TCCGGTCTGC ACATCATCTA CACGCAGGAT 
GAGGTGGACG TCGTTCAGAA CCTCATGTTC TACATCGAGG CAGCTTACAA AGTGGCTGAT 
TATGGGATGT GGGAGAGGGG GGACAAAACC AACCAGGGCA TCACAGAGAT CAATGCCAGC 
TCCATCGGCA TGGCCAAGGC GGCTCTGGAG GCCCTGGACG AACTCAACCT GTTTGGAGCT 
AAGGGAGGAC CGGGATCTGT GGTCCACGCT CTGGCTGATG ACATCCAGCA CTGCCAGTCC 
ATCCTCACAT CCATGTTACC CAAAGCTTCC ATCTCTAAAG AGGTGGATGC CGGAGTTTTG 
GCTATTCTTA CCTACCCCGC CTTCGCCGTG GAGGACATGA GCATCGTCAC CATGACCAAG 
GAGGAGATCA TCTCCAAGCT GCAGGGTCGG TACGGCTGTT GCAGGTTTCT CAGGGACGGA 
CATAAAACAC CCAAAGAGGA TCCCAACCGC CTGTACTACG AGTCTGCAGA GCTGAAGCTG 
TTCGAGAACA TCGAGTGTGA GTGGCCTCTC TTCTGGACGT ACCTCATTCT GGACGGCATA 
TTCAGCAACA GTCATGAACA GGTGCAGGAG TACCAGGAGG CGCTGGAGGG CATCCTCATC 
AAGCAGAAGG ATGGCATACG ACTGTTTCCA GAGCTCTACA GCGTTCCTCC AGATAAGGTG 
GAAGAGGAAT ACATGAACCC CCACACAGTG GAGAGGATCC CGATGGGGAA ATGTCCCCTG 
AAATGGGGCC AGTCCCTTTA TGTTCTGGGG AGACTCTTAT CCGAGGGGTT TCTTGCTCCT 
GGTGAGATTG ACCCTCTTAA CCGCCGTTTC TCCACCGTGC CCAAGCCGGA CGTGGTCGTT 
CAGGTTTCCA TTTTGGCTGA AACAGATGAG ATTAAGGAGC TTTTGCTGAA GAACGGCTTC 
GAGGTGGAAA CCATCGCAGA CATCCACCCC TTTCATGTGC AGCCCTCCAG AGTCCTCAGC 
CACATCTATG CTAGGCTCGG ACGTAACCCA AGGCTGGGCC TGACGGGACG GCCCTACAGG 
AGAATCGGAG TTCTGGGAAC CTCAAAGTTC TACATCATCA GAAACACCAT CTTCACATTC 
ACTCCACAGT TCATCGACCA CCAGCAGTTC TACCTGGCTC TGGACAACAA AATGATCGTA 
GAGATGTTGA GGACAGAAAT CGCCTACCTG TCCTCCAGGT GGAGGATAAC CGGACGGCCG 
ACGGTCACCT TCCCCGTCTC TCAAACCATG CTGACTGAAG ACCGCACAGA TCTGGACCCG 
GCTGTCCTGG CGACGCTGAA GAAGCTACAG GATGGGTATT TTGGAGGAGC CAGGATCCAG 
ACGGGAAAGC TGTCAGAGTT CCTGACCACC TCCTGCTGCG CTCACCTGAG CTTCCTGGAT 
GGTAAGCGCT TCGGCAGCAT GGGTTCCCAC GCCGAAGAGT ATGACCATGA TGATGCCAGC 
GATGGATATG TGCATGAGTT GCATTGTGAT GATGAGGCAG ACGACCTTGC TCAGTACTTG 
GATCACCTCC TGGCTCACTC TGCTTCTAAG AAGCAGAAGT CCAGCGCTGA AGGTGTGGGC 
CGATTCAAAG CTGTTGCTAC AAGAACCAGG GAGATGGTCT CTTTGATGAA CAAGGCTCAG 
GATCTTAACA TCCACAACAT TGGCATGTAC CTTCCCAACA AGCTGTTTCG TACTCGTCAT 
CCATCGCTCA ATCTGAACCT TCCAGACAGT ACTGTCCACG AAGAAGCTCA GGCACCAGAG 
GTGTCCATCA CCACAGAAAG TAGCCTCCCC AGAGATGCCA GCGGCGCCAT CGACTACAGT 
GCTTTGGTCC AGCTGCTGAA AGACAGTGGG AACATCCAGG ACCAGGCAGA TATTCTCTAC 
ATCCTCTTCA AAGACAAGGG GATGGACTGG GACACACACC TTCATGGGAG GGGCTCCACT 
GTCCGGTCTC TGCTCAGCAA CCTTTACGAG AAGGCTGGTG ACCTCAAACA CTGGGGCCTG 
ATCCGGATGA TCTCTGGGAT GCTGAAGAAG AAGGTGGAAG AGCTCGACTC GGCCTGCTCT 
GACCTGCTGG CTCACCAGAA GCACCTGACA GTAGGTCTGC CTCCAGAACC CAGGGAGAAA 
ACCATCACGG CGCCCATCCC TCCAGACCAG CTGGCTGCCC TCATCGATGA GGCCAGCGAT 
GGCAACATCA GTGTTGCCAT CCTCACACAG GAGATCATGG TGTTCCTGGC CATGAGCATC 
CGCACGCAGC CCAGTCTGTT CAGCGAGATG TTCAGGCTCC GGATCGGCCT CATCATTCAA 
GTCATGGCCA CAGAACTGGC TCAGTCCCTC AACTGCTCTG GTGACGAGGC CACAGAGAGT 
CTGATGAGTC TGAGTCCGTC AGAGCTGAAG AACCTGCTCC ACCACATCCT CAGTGGGAAA 
GAGTTTGGCG TGGAGCGCAG CGTCAGAGAG GGAGATGATG GCATCAGTCC TGCCATCTCC 
ATCCACCACC TGGGGAACGT TGGCGCCACC AAGAGTGAGA GAGCGGGCAT CAGCAAGCTG 
AAGAGCGACA TAAAGATGCT TGAGCACACG ATGTCTCTGT CTGTGAGTAT GATAAAAAAT 
GATCATCTTA TTGGTGCTAA TTCACCTGAT GGAAGGGAAG GAGAGCTTGA GATGCATGGG 
GCAAATCATG ACGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phka1 ( XM_020711742.1 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_020567401.1 Oryzias latipes phosphorylase kinase regulatory subunit alpha 1 (phka1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OJa74016
Clone ID Related Accession (Same CDS sequence)	XM_020711742.2 , XM_020711742.1
Accession Version	XM_020711742.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3138bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-06
Organism	Oryzias latipes(Japanese medaka)
Product	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019876.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryzias latipes Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGCC GAAGCAACTC CGGCGTGAAG CTGGACAACT ATGCCCGGAT CGTCCAGCAG 
ACCATCCTGC AGCACCAGGA TCCAGTAACG GGTCTCCTTC CGGGCAGCTT GGAGCAGCCA 
GATGCATGGG TGAGGGACAA CGTCTACAGC ATCGTCTCAG TGTGGGCCCT CAGCCTGGCC 
TACAGGAAAA ATGCCGACCG GGACGAGGAC AAGGCCAAGG CCTACGAGCT GGAGCAGAGT 
GTGGTGAAGC TCATGAGAGG TGTCCTCCAG TGCATTATGA GACAGCTAGA CAAAGTGGAG 
AAGTTCAAAT ACAGCCGAAG TACCAAGGAC TCACTTCACG CCAAGTACAA CCCACGCACC 
TGTGCTACAG TGGTCGGGGA CCACCAGTGG GGTCACCTGC AGGTGGACGC AACCTCGCTC 
TTCCTGCTCT TCCTCGCTCA GATGACGGCA TCCGGTCTGC ACATCATCTA CACGCAGGAT 
GAGGTGGACG TCGTTCAGAA CCTCATGTTC TACATCGAGG CAGCTTACAA AGTGGCTGAT 
TATGGGATGT GGGAGAGGGG GGACAAAACC AACCAGGGCA TCACAGAGAT CAATGCCAGC 
TCCATCGGCA TGGCCAAGGC GGCTCTGGAG GCCCTGGACG AACTCAACCT GTTTGGAGCT 
AAGGGAGGAC CGGGATCTGT GGTCCACGCT CTGGCTGATG ACATCCAGCA CTGCCAGTCC 
ATCCTCACAT CCATGTTACC CAAAGCTTCC ATCTCTAAAG AGGTGGATGC CGGAGTTTTG 
GCTATTCTTA CCTACCCCGC CTTCGCCGTG GAGGACATGA GCATCGTCAC CATGACCAAG 
GAGGAGATCA TCTCCAAGCT GCAGGGTCGG TACGGCTGTT GCAGGTTTCT CAGGGACGGA 
CATAAAACAC CCAAAGAGGA TCCCAACCGC CTGTACTACG AGTCTGCAGA GCTGAAGCTG 
TTCGAGAACA TCGAGTGTGA GTGGCCTCTC TTCTGGACGT ACCTCATTCT GGACGGCATA 
TTCAGCAACA GTCATGAACA GGTGCAGGAG TACCAGGAGG CGCTGGAGGG CATCCTCATC 
AAGCAGAAGG ATGGCATACG ACTGTTTCCA GAGCTCTACA GCGTTCCTCC AGATAAGGTG 
GAAGAGGAAT ACATGAACCC CCACACAGTG GAGAGGATCC CGATGGGGAA ATGTCCCCTG 
AAATGGGGCC AGTCCCTTTA TGTTCTGGGG AGACTCTTAT CCGAGGGGTT TCTTGCTCCT 
GGTGAGATTG ACCCTCTTAA CCGCCGTTTC TCCACCGTGC CCAAGCCGGA CGTGGTCGTT 
CAGGTTTCCA TTTTGGCTGA AACAGATGAG ATTAAGGAGC TTTTGCTGAA GAACGGCTTC 
GAGGTGGAAA CCATCGCAGA CATCCACCCC TTTCATGTGC AGCCCTCCAG AGTCCTCAGC 
CACATCTATG CTAGGCTCGG ACGTAACCCA AGGCTGGGCC TGACGGGACG GCCCTACAGG 
AGAATCGGAG TTCTGGGAAC CTCAAAGTTC TACATCATCA GAAACACCAT CTTCACATTC 
ACTCCACAGT TCATCGACCA CCAGCAGTTC TACCTGGCTC TGGACAACAA AATGATCGTA 
GAGATGTTGA GGACAGAAAT CGCCTACCTG TCCTCCAGGT GGAGGATAAC CGGACGGCCG 
ACGGTCACCT TCCCCGTCTC TCAAACCATG CTGACTGAAG ACCGCACAGA TCTGGACCCG 
GCTGTCCTGG CGACGCTGAA GAAGCTACAG GATGGGTATT TTGGAGGAGC CAGGATCCAG 
ACGGGAAAGC TGTCAGAGTT CCTGACCACC TCCTGCTGCG CTCACCTGAG CTTCCTGGAT 
GGTAAGCGCT TCGGCAGCAT GGGTTCCCAC GCCGAAGAGT ATGACCATGA TGATGCCAGC 
GATGGATATG TGCATGAGTT GCATTGTGAT GATGAGGCAG ACGACCTTGC TCAGTACTTG 
GATCACCTCC TGGCTCACTC TGCTTCTAAG AAGCAGAAGT CCAGCGCTGA AGGTGTGGGC 
CGATTCAAAG CTGTTGCTAC AAGAACCAGG GAGATGGTCT CTTTGATGAA CAAGGCTCAG 
GATCTTAACA TCCACAACAT TGGCATGTAC CTTCCCAACA AGCTGTTTCG TACTCGTCAT 
CCATCGCTCA ATCTGAACCT TCCAGACAGT ACTGTCCACG AAGAAGCTCA GGCACCAGAG 
GTGTCCATCA CCACAGAAAG TAGCCTCCCC AGAGATGCCA GCGGCGCCAT CGACTACAGT 
GCTTTGGTCC AGCTGCTGAA AGACAGTGGG AACATCCAGG ACCAGGCAGA TATTCTCTAC 
ATCCTCTTCA AAGACAAGGG GATGGACTGG GACACACACC TTCATGGGAG GGGCTCCACT 
GTCCGGTCTC TGCTCAGCAA CCTTTACGAG AAGGCTGGTG ACCTCAAACA CTGGGGCCTG 
ATCCGGATGA TCTCTGGGAT GCTGAAGAAG AAGGTGGAAG AGCTCGACTC GGCCTGCTCT 
GACCTGCTGG CTCACCAGAA GCACCTGACA GTAGGTCTGC CTCCAGAACC CAGGGAGAAA 
ACCATCACGG CGCCCATCCC TCCAGACCAG CTGGCTGCCC TCATCGATGA GGCCAGCGAT 
GGCAACATCA GTGTTGCCAT CCTCACACAG GAGATCATGG TGTTCCTGGC CATGAGCATC 
CGCACGCAGC CCAGTCTGTT CAGCGAGATG TTCAGGCTCC GGATCGGCCT CATCATTCAA 
GTCATGGCCA CAGAACTGGC TCAGTCCCTC AACTGCTCTG GTGACGAGGC CACAGAGAGT 
CTGATGAGTC TGAGTCCGTC AGAGCTGAAG AACCTGCTCC ACCACATCCT CAGTGGGAAA 
GAGTTTGGCG TGGAGCGCAG CGTCAGAGAG GGAGATGATG GCATCAGTCC TGCCATCTCC 
ATCCACCACC TGGGGAACGT TGGCGCCACC AAGAGTGAGA GAGCGGGCAT CAGCAAGCTG 
AAGAGCGACA TAAAGATGCT TGAGCACACG ATGTCTCTGT CTGTGAGTAT GATAAAAAAT 
GATCATCTTA TTGGTGCTAA TTCACCTGAT GGAAGGGAAG GAGAGCTTGA GATGCATGGG 
GCAAATCATG ACGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020567401.1
CDS	195..3332
Translation	MRSRSNSGVKLDNYARIVQQTILQHQDPVTGLLPGSLEQPDAWVRDNVYSIVSVWALSLAYRKNADRDEDKAKAYELEQSVVKLMRGVLQCIMRQLDKVEKFKYSRSTKDSLHAKYNPRTCATVVGDHQWGHLQVDATSLFLLFLAQMTASGLHIIYTQDEVDVVQNLMFYIEAAYKVADYGMWERGDKTNQGITEINASSIGMAKAALEALDELNLFGAKGGPGSVVHALADDIQHCQSILTSMLPKASISKEVDAGVLAILTYPAFAVEDMSIVTMTKEEIISKLQGRYGCCRFLRDGHKTPKEDPNRLYYESAELKLFENIECEWPLFWTYLILDGIFSNSHEQVQEYQEALEGILIKQKDGIRLFPELYSVPPDKVEEEYMNPHTVERIPMGKCPLKWGQSLYVLGRLLSEGFLAPGEIDPLNRRFSTVPKPDVVVQVSILAETDEIKELLLKNGFEVETIADIHPFHVQPSRVLSHIYARLGRNPRLGLTGRPYRRIGVLGTSKFYIIRNTIFTFTPQFIDHQQFYLALDNKMIVEMLRTEIAYLSSRWRITGRPTVTFPVSQTMLTEDRTDLDPAVLATLKKLQDGYFGGARIQTGKLSEFLTTSCCAHLSFLDGKRFGSMGSHAEEYDHDDASDGYVHELHCDDEADDLAQYLDHLLAHSASKKQKSSAEGVGRFKAVATRTREMVSLMNKAQDLNIHNIGMYLPNKLFRTRHPSLNLNLPDSTVHEEAQAPEVSITTESSLPRDASGAIDYSALVQLLKDSGNIQDQADILYILFKDKGMDWDTHLHGRGSTVRSLLSNLYEKAGDLKHWGLIRMISGMLKKKVEELDSACSDLLAHQKHLTVGLPPEPREKTITAPIPPDQLAALIDEASDGNISVAILTQEIMVFLAMSIRTQPSLFSEMFRLRIGLIIQVMATELAQSLNCSGDEATESLMSLSPSELKNLLHHILSGKEFGVERSVREGDDGISPAISIHHLGNVGATKSERAGISKLKSDIKMLEHTMSLSVSMIKNDHLIGANSPDGREGELEMHGANHDA

Target ORF information:

RefSeq Version	XM_020711742.1
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes phosphorylase kinase regulatory subunit alpha 1 (phka1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020711742.1

ORF Insert Sequence:

ATGAGGAGCC GAAGCAACTC CGGCGTGAAG CTGGACAACT ATGCCCGGAT CGTCCAGCAG 
ACCATCCTGC AGCACCAGGA TCCAGTAACG GGTCTCCTTC CGGGCAGCTT GGAGCAGCCA 
GATGCATGGG TGAGGGACAA CGTCTACAGC ATCGTCTCAG TGTGGGCCCT CAGCCTGGCC 
TACAGGAAAA ATGCCGACCG GGACGAGGAC AAGGCCAAGG CCTACGAGCT GGAGCAGAGT 
GTGGTGAAGC TCATGAGAGG TGTCCTCCAG TGCATTATGA GACAGCTAGA CAAAGTGGAG 
AAGTTCAAAT ACAGCCGAAG TACCAAGGAC TCACTTCACG CCAAGTACAA CCCACGCACC 
TGTGCTACAG TGGTCGGGGA CCACCAGTGG GGTCACCTGC AGGTGGACGC AACCTCGCTC 
TTCCTGCTCT TCCTCGCTCA GATGACGGCA TCCGGTCTGC ACATCATCTA CACGCAGGAT 
GAGGTGGACG TCGTTCAGAA CCTCATGTTC TACATCGAGG CAGCTTACAA AGTGGCTGAT 
TATGGGATGT GGGAGAGGGG GGACAAAACC AACCAGGGCA TCACAGAGAT CAATGCCAGC 
TCCATCGGCA TGGCCAAGGC GGCTCTGGAG GCCCTGGACG AACTCAACCT GTTTGGAGCT 
AAGGGAGGAC CGGGATCTGT GGTCCACGCT CTGGCTGATG ACATCCAGCA CTGCCAGTCC 
ATCCTCACAT CCATGTTACC CAAAGCTTCC ATCTCTAAAG AGGTGGATGC CGGAGTTTTG 
GCTATTCTTA CCTACCCCGC CTTCGCCGTG GAGGACATGA GCATCGTCAC CATGACCAAG 
GAGGAGATCA TCTCCAAGCT GCAGGGTCGG TACGGCTGTT GCAGGTTTCT CAGGGACGGA 
CATAAAACAC CCAAAGAGGA TCCCAACCGC CTGTACTACG AGTCTGCAGA GCTGAAGCTG 
TTCGAGAACA TCGAGTGTGA GTGGCCTCTC TTCTGGACGT ACCTCATTCT GGACGGCATA 
TTCAGCAACA GTCATGAACA GGTGCAGGAG TACCAGGAGG CGCTGGAGGG CATCCTCATC 
AAGCAGAAGG ATGGCATACG ACTGTTTCCA GAGCTCTACA GCGTTCCTCC AGATAAGGTG 
GAAGAGGAAT ACATGAACCC CCACACAGTG GAGAGGATCC CGATGGGGAA ATGTCCCCTG 
AAATGGGGCC AGTCCCTTTA TGTTCTGGGG AGACTCTTAT CCGAGGGGTT TCTTGCTCCT 
GGTGAGATTG ACCCTCTTAA CCGCCGTTTC TCCACCGTGC CCAAGCCGGA CGTGGTCGTT 
CAGGTTTCCA TTTTGGCTGA AACAGATGAG ATTAAGGAGC TTTTGCTGAA GAACGGCTTC 
GAGGTGGAAA CCATCGCAGA CATCCACCCC TTTCATGTGC AGCCCTCCAG AGTCCTCAGC 
CACATCTATG CTAGGCTCGG ACGTAACCCA AGGCTGGGCC TGACGGGACG GCCCTACAGG 
AGAATCGGAG TTCTGGGAAC CTCAAAGTTC TACATCATCA GAAACACCAT CTTCACATTC 
ACTCCACAGT TCATCGACCA CCAGCAGTTC TACCTGGCTC TGGACAACAA AATGATCGTA 
GAGATGTTGA GGACAGAAAT CGCCTACCTG TCCTCCAGGT GGAGGATAAC CGGACGGCCG 
ACGGTCACCT TCCCCGTCTC TCAAACCATG CTGACTGAAG ACCGCACAGA TCTGGACCCG 
GCTGTCCTGG CGACGCTGAA GAAGCTACAG GATGGGTATT TTGGAGGAGC CAGGATCCAG 
ACGGGAAAGC TGTCAGAGTT CCTGACCACC TCCTGCTGCG CTCACCTGAG CTTCCTGGAT 
GGTAAGCGCT TCGGCAGCAT GGGTTCCCAC GCCGAAGAGT ATGACCATGA TGATGCCAGC 
GATGGATATG TGCATGAGTT GCATTGTGAT GATGAGGCAG ACGACCTTGC TCAGTACTTG 
GATCACCTCC TGGCTCACTC TGCTTCTAAG AAGCAGAAGT CCAGCGCTGA AGGTGTGGGC 
CGATTCAAAG CTGTTGCTAC AAGAACCAGG GAGATGGTCT CTTTGATGAA CAAGGCTCAG 
GATCTTAACA TCCACAACAT TGGCATGTAC CTTCCCAACA AGCTGTTTCG TACTCGTCAT 
CCATCGCTCA ATCTGAACCT TCCAGACAGT ACTGTCCACG AAGAAGCTCA GGCACCAGAG 
GTGTCCATCA CCACAGAAAG TAGCCTCCCC AGAGATGCCA GCGGCGCCAT CGACTACAGT 
GCTTTGGTCC AGCTGCTGAA AGACAGTGGG AACATCCAGG ACCAGGCAGA TATTCTCTAC 
ATCCTCTTCA AAGACAAGGG GATGGACTGG GACACACACC TTCATGGGAG GGGCTCCACT 
GTCCGGTCTC TGCTCAGCAA CCTTTACGAG AAGGCTGGTG ACCTCAAACA CTGGGGCCTG 
ATCCGGATGA TCTCTGGGAT GCTGAAGAAG AAGGTGGAAG AGCTCGACTC GGCCTGCTCT 
GACCTGCTGG CTCACCAGAA GCACCTGACA GTAGGTCTGC CTCCAGAACC CAGGGAGAAA 
ACCATCACGG CGCCCATCCC TCCAGACCAG CTGGCTGCCC TCATCGATGA GGCCAGCGAT 
GGCAACATCA GTGTTGCCAT CCTCACACAG GAGATCATGG TGTTCCTGGC CATGAGCATC 
CGCACGCAGC CCAGTCTGTT CAGCGAGATG TTCAGGCTCC GGATCGGCCT CATCATTCAA 
GTCATGGCCA CAGAACTGGC TCAGTCCCTC AACTGCTCTG GTGACGAGGC CACAGAGAGT 
CTGATGAGTC TGAGTCCGTC AGAGCTGAAG AACCTGCTCC ACCACATCCT CAGTGGGAAA 
GAGTTTGGCG TGGAGCGCAG CGTCAGAGAG GGAGATGATG GCATCAGTCC TGCCATCTCC 
ATCCACCACC TGGGGAACGT TGGCGCCACC AAGAGTGAGA GAGCGGGCAT CAGCAAGCTG 
AAGAGCGACA TAAAGATGCT TGAGCACACG ATGTCTCTGT CTGTGAGTAT GATAAAAAAT 
GATCATCTTA TTGGTGCTAA TTCACCTGAT GGAAGGGAAG GAGAGCTTGA GATGCATGGG 
GCAAATCATG ACGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phka1 ( XM_011487671.2 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_011485973.1 Oryzias latipes phosphorylase kinase regulatory subunit alpha 1 (phka1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OJa32967
Clone ID Related Accession (Same CDS sequence)	XM_011487671.2 , XM_011487671.3
Accession Version	XM_011487671.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3654bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-06
Organism	Oryzias latipes(Japanese medaka)
Product	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019876.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 7, 2017 this sequence version replaced XM_011487671.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryzias latipes Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGCC GAAGCAACTC CGGCGTGAAG CTGGACAACT ATGCCCGGAT CGTCCAGCAG 
ACCATCCTGC AGCACCAGGA TCCAGTAACG GGTCTCCTTC CGGGCAGCTT GGAGCAGCCA 
GATGCATGGG TGAGGGACAA CGTCTACAGC ATCGTCTCAG TGTGGGCCCT CAGCCTGGCC 
TACAGGAAAA ATGCCGACCG GGACGAGGAC AAGGCCAAGG CCTACGAGCT GGAGCAGAGT 
GTGGTGAAGC TCATGAGAGG TGTCCTCCAG TGCATTATGA GACAGCTAGA CAAAGTGGAG 
AAGTTCAAAT ACAGCCGAAG TACCAAGGAC TCACTTCACG CCAAGTACAA CCCACGCACC 
TGTGCTACAG TGGTCGGGGA CCACCAGTGG GGTCACCTGC AGGTGGACGC AACCTCGCTC 
TTCCTGCTCT TCCTCGCTCA GATGACGGCA TCCGGTCTGC ACATCATCTA CACGCAGGAT 
GAGGTGGACG TCGTTCAGAA CCTCATGTTC TACATCGAGG CAGCTTACAA AGTGGCTGAT 
TATGGGATGT GGGAGAGGGG GGACAAAACC AACCAGGGCA TCACAGAGAT CAATGCCAGC 
TCCATCGGCA TGGCCAAGGC GGCTCTGGAG GCCCTGGACG AACTCAACCT GTTTGGAGCT 
AAGGGAGGAC CGGGATCTGT GGTCCACGCT CTGGCTGATG ACATCCAGCA CTGCCAGTCC 
ATCCTCACAT CCATGTTACC CAAAGCTTCC ATCTCTAAAG AGGTGGATGC CGGAGTTTTG 
GCTATTCTTA CCTACCCCGC CTTCGCCGTG GAGGACATGA GCATCGTCAC CATGACCAAG 
GAGGAGATCA TCTCCAAGCT GCAGGGTCGG TACGGCTGTT GCAGGTTTCT CAGGGACGGA 
CATAAAACAC CCAAAGAGGA TCCCAACCGC CTGTACTACG AGTCTGCAGA GCTGAAGCTG 
TTCGAGAACA TCGAGTGTGA GTGGCCTCTC TTCTGGACGT ACCTCATTCT GGACGGCATA 
TTCAGCAACA GTCATGAACA GGTGCAGGAG TACCAGGAGG CGCTGGAGGG CATCCTCATC 
AAGCAGAAGG ATGGCATACG ACTGTTTCCA GAGCTCTACA GCGTTCCTCC AGATAAGGTG 
GAAGAGGAAT ACATGAACCC CCACACAGTG GAGAGGATCC CGATGGGGAA ATGTCCCCTG 
AAATGGGGCC AGTCCCTTTA TGTTCTGGGG AGACTCTTAT CCGAGGGGTT TCTTGCTCCT 
GGTGAGATTG ACCCTCTTAA CCGCCGTTTC TCCACCGTGC CCAAGCCGGA CGTGGTCGTT 
CAGGTTTCCA TTTTGGCTGA AACAGATGAG ATTAAGGAGC TTTTGCTGAA GAACGGCTTC 
GAGGTGGAAA CCATCGCAGA CATCCACCCC TTTCATGTGC AGCCCTCCAG AGTCCTCAGC 
CACATCTATG CTAGGCTCGG ACGTAACCCA AGGCTGGGCC TGACGGGACG GCCCTACAGG 
AGAATCGGAG TTCTGGGAAC CTCAAAGTTC TACATCATCA GAAACACCAT CTTCACATTC 
ACTCCACAGT TCATCGACCA CCAGCAGTTC TACCTGGCTC TGGACAACAA AATGATCGTA 
GAGATGTTGA GGACAGAAAT CGCCTACCTG TCCTCCAGGT GGAGGATAAC CGGACGGCCG 
ACGGTCACCT TCCCCGTCTC TCAAACCATG CTGACTGAAG ACCGCACAGA TCTGGACCCG 
GCTGTCCTGG CGACGCTGAA GAAGCTACAG GATGGGTATT TTGGAGGAGC CAGGATCCAG 
ACGGGAAAGC TGTCAGAGTT CCTGACCACC TCCTGCTGCG CTCACCTGAG CTTCCTGGAT 
GGTAAGCGCT TCGGCAGCAT GGGTTCCCAC GCCGAAGAGT ATGACCATGA TGATGCCAGC 
GATGGATATG TGCATGAGTT GCATTGTGAT GATGAGGCAG ACGACCTTGC TCAGTACTTG 
GATCACCTCC TGGCTCACTC TGCTTCTAAG AAGCAGAAGT CCAGCGCTGA AGGTGTGGGC 
CGATTCAAAG CTGTTGCTAC AAGAACCAGG GAGATGGTCT CTTTGATGAA CAAGGCTCAG 
GATCTTAACA TCCACAACAT TGGCATGTAC CTTCCCAACA AGCTGTTTCG TACTCGTCAT 
CCATCGCTCA ATCTGAACCT TCCAGACAGT ACTGTCCACG AAGAAGCTCA GGCACCAGAG 
GTGTCCATCA CCACAGAAAG TAGCCTCCCC AGAGATGCCA GCGGCGCCAT CGACTACAGT 
GCTTTGGTCC AGCTGCTGAA AGACAGTGGG AACATCCAGG ACCAGGCAGA TATTCTCTAC 
ATCCTCTTCA AAGACAAGGG GATGGACTGG GACACACACC TTCATGGGAG GGGCTCCACT 
GTCCGGTCTC TGCTCAGCAA CCTTTACGAG AAGGCTGGTG ACCTCAAACA CTGGGGCCTG 
ATCCGGATGA TCTCTGGGAT GCTGAAGAAG AAGGTGGAAG AGCTCGACTC GGCCTGCTCT 
GACCTGCTGG CTCACCAGAA GCACCTGACA GTAGGTCTGC CTCCAGAACC CAGGGAGAAA 
ACCATCACGG CGCCCATCCC TCCAGACCAG CTGGCTGCCC TCATCGATGA GGCCAGCGAT 
GGCAACATCA GTGTTGCCAT CCTCACACAG GAGATCATGG TGTTCCTGGC CATGAGCATC 
CGCACGCAGC CCAGTCTGTT CAGCGAGATG TTCAGGCTCC GGATCGGCCT CATCATTCAA 
GTCATGGCCA CAGAACTGGC TCAGTCCCTC AACTGCTCTG GTGACGAGGC CACAGAGAGT 
CTGATGAGTC TGAGTCCGTC AGAGCTGAAG AACCTGCTCC ACCACATCCT CAGTGGGAAA 
GAGTTTGGCG TGGAGCGCAG CGTCAGAGAG GGAGATGATG GCATCAGTCC TGCCATCTCC 
ATCCACCACC TGGGGAACGT TGGCGCCACC AAGAGTGAGA GAGCGGGCAT CAGCAAGCTG 
AAGAGCGACA TAAAGATGGG CGTTCAAAGT CCGTCGCTGG ATATGGAAAA TGTGGAATCG 
GGGAGGTACC GGCTGCCGTC CATAGAGTCT CTGGACATTT CGGACAGCAT TCCTACCATC 
AAGGACACCA GACATGGACA GTGGCTGCGC AGGAGACGGC TGGACGGCGC GCTGAACCGA 
GTTCCTGTGG GCTTCTACCA GAAAGTGTGG AGGATCCTGC AGAAGTGCCA CGGTCTGTCC 
ATCGAGGGCT TTGTTCTTCC ATCATCCACC ACCAGAGAGA TGACCCCCGC AGAAATCAAG 
TTCTCAGTTC ACGTGGAAAC CGTTCTGAAC CGCGTCCCCC AGCCCGAGTA CCGGCAGCTG 
CTGGTGGAAG CCATTCTGGT CCTCACCATG CTGGCCGACG TGGACATCCC AAGCATCGGC 
TCCATCATCC ATGTGGAGAA GATCGTCCAT TTGGCCAACG ATATGTTCTA CAAAGATCAG 
AAGGACCTGG GGGCCGAGGG GCACATCCTA GAAAAAGATC CGTCCACGGG AGTGTGCCGA 
CTCCTGTATG ACAGCGCCCC CAGTGGTCGC TTTGGAAGCA TGACCTACCT GACCAAGGCG 
GTGGCGGCTT ATGTACAGGA CTTCCTGCCT AGCGGGGCGT GTGCCGTGCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011485973.1
CDS	192..3845
Translation	MRSRSNSGVKLDNYARIVQQTILQHQDPVTGLLPGSLEQPDAWVRDNVYSIVSVWALSLAYRKNADRDEDKAKAYELEQSVVKLMRGVLQCIMRQLDKVEKFKYSRSTKDSLHAKYNPRTCATVVGDHQWGHLQVDATSLFLLFLAQMTASGLHIIYTQDEVDVVQNLMFYIEAAYKVADYGMWERGDKTNQGITEINASSIGMAKAALEALDELNLFGAKGGPGSVVHALADDIQHCQSILTSMLPKASISKEVDAGVLAILTYPAFAVEDMSIVTMTKEEIISKLQGRYGCCRFLRDGHKTPKEDPNRLYYESAELKLFENIECEWPLFWTYLILDGIFSNSHEQVQEYQEALEGILIKQKDGIRLFPELYSVPPDKVEEEYMNPHTVERIPMGKCPLKWGQSLYVLGRLLSEGFLAPGEIDPLNRRFSTVPKPDVVVQVSILAETDEIKELLLKNGFEVETIADIHPFHVQPSRVLSHIYARLGRNPRLGLTGRPYRRIGVLGTSKFYIIRNTIFTFTPQFIDHQQFYLALDNKMIVEMLRTEIAYLSSRWRITGRPTVTFPVSQTMLTEDRTDLDPAVLATLKKLQDGYFGGARIQTGKLSEFLTTSCCAHLSFLDGKRFGSMGSHAEEYDHDDASDGYVHELHCDDEADDLAQYLDHLLAHSASKKQKSSAEGVGRFKAVATRTREMVSLMNKAQDLNIHNIGMYLPNKLFRTRHPSLNLNLPDSTVHEEAQAPEVSITTESSLPRDASGAIDYSALVQLLKDSGNIQDQADILYILFKDKGMDWDTHLHGRGSTVRSLLSNLYEKAGDLKHWGLIRMISGMLKKKVEELDSACSDLLAHQKHLTVGLPPEPREKTITAPIPPDQLAALIDEASDGNISVAILTQEIMVFLAMSIRTQPSLFSEMFRLRIGLIIQVMATELAQSLNCSGDEATESLMSLSPSELKNLLHHILSGKEFGVERSVREGDDGISPAISIHHLGNVGATKSERAGISKLKSDIKMGVQSPSLDMENVESGRYRLPSIESLDISDSIPTIKDTRHGQWLRRRRLDGALNRVPVGFYQKVWRILQKCHGLSIEGFVLPSSTTREMTPAEIKFSVHVETVLNRVPQPEYRQLLVEAILVLTMLADVDIPSIGSIIHVEKIVHLANDMFYKDQKDLGAEGHILEKDPSTGVCRLLYDSAPSGRFGSMTYLTKAVAAYVQDFLPSGACAVQ

Target ORF information:

RefSeq Version	XM_011487671.2
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes phosphorylase kinase regulatory subunit alpha 1 (phka1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011487671.2

ORF Insert Sequence:

ATGAGGAGCC GAAGCAACTC CGGCGTGAAG CTGGACAACT ATGCCCGGAT CGTCCAGCAG 
ACCATCCTGC AGCACCAGGA TCCAGTAACG GGTCTCCTTC CGGGCAGCTT GGAGCAGCCA 
GATGCATGGG TGAGGGACAA CGTCTACAGC ATCGTCTCAG TGTGGGCCCT CAGCCTGGCC 
TACAGGAAAA ATGCCGACCG GGACGAGGAC AAGGCCAAGG CCTACGAGCT GGAGCAGAGT 
GTGGTGAAGC TCATGAGAGG TGTCCTCCAG TGCATTATGA GACAGCTAGA CAAAGTGGAG 
AAGTTCAAAT ACAGCCGAAG TACCAAGGAC TCACTTCACG CCAAGTACAA CCCACGCACC 
TGTGCTACAG TGGTCGGGGA CCACCAGTGG GGTCACCTGC AGGTGGACGC AACCTCGCTC 
TTCCTGCTCT TCCTCGCTCA GATGACGGCA TCCGGTCTGC ACATCATCTA CACGCAGGAT 
GAGGTGGACG TCGTTCAGAA CCTCATGTTC TACATCGAGG CAGCTTACAA AGTGGCTGAT 
TATGGGATGT GGGAGAGGGG GGACAAAACC AACCAGGGCA TCACAGAGAT CAATGCCAGC 
TCCATCGGCA TGGCCAAGGC GGCTCTGGAG GCCCTGGACG AACTCAACCT GTTTGGAGCT 
AAGGGAGGAC CGGGATCTGT GGTCCACGCT CTGGCTGATG ACATCCAGCA CTGCCAGTCC 
ATCCTCACAT CCATGTTACC CAAAGCTTCC ATCTCTAAAG AGGTGGATGC CGGAGTTTTG 
GCTATTCTTA CCTACCCCGC CTTCGCCGTG GAGGACATGA GCATCGTCAC CATGACCAAG 
GAGGAGATCA TCTCCAAGCT GCAGGGTCGG TACGGCTGTT GCAGGTTTCT CAGGGACGGA 
CATAAAACAC CCAAAGAGGA TCCCAACCGC CTGTACTACG AGTCTGCAGA GCTGAAGCTG 
TTCGAGAACA TCGAGTGTGA GTGGCCTCTC TTCTGGACGT ACCTCATTCT GGACGGCATA 
TTCAGCAACA GTCATGAACA GGTGCAGGAG TACCAGGAGG CGCTGGAGGG CATCCTCATC 
AAGCAGAAGG ATGGCATACG ACTGTTTCCA GAGCTCTACA GCGTTCCTCC AGATAAGGTG 
GAAGAGGAAT ACATGAACCC CCACACAGTG GAGAGGATCC CGATGGGGAA ATGTCCCCTG 
AAATGGGGCC AGTCCCTTTA TGTTCTGGGG AGACTCTTAT CCGAGGGGTT TCTTGCTCCT 
GGTGAGATTG ACCCTCTTAA CCGCCGTTTC TCCACCGTGC CCAAGCCGGA CGTGGTCGTT 
CAGGTTTCCA TTTTGGCTGA AACAGATGAG ATTAAGGAGC TTTTGCTGAA GAACGGCTTC 
GAGGTGGAAA CCATCGCAGA CATCCACCCC TTTCATGTGC AGCCCTCCAG AGTCCTCAGC 
CACATCTATG CTAGGCTCGG ACGTAACCCA AGGCTGGGCC TGACGGGACG GCCCTACAGG 
AGAATCGGAG TTCTGGGAAC CTCAAAGTTC TACATCATCA GAAACACCAT CTTCACATTC 
ACTCCACAGT TCATCGACCA CCAGCAGTTC TACCTGGCTC TGGACAACAA AATGATCGTA 
GAGATGTTGA GGACAGAAAT CGCCTACCTG TCCTCCAGGT GGAGGATAAC CGGACGGCCG 
ACGGTCACCT TCCCCGTCTC TCAAACCATG CTGACTGAAG ACCGCACAGA TCTGGACCCG 
GCTGTCCTGG CGACGCTGAA GAAGCTACAG GATGGGTATT TTGGAGGAGC CAGGATCCAG 
ACGGGAAAGC TGTCAGAGTT CCTGACCACC TCCTGCTGCG CTCACCTGAG CTTCCTGGAT 
GGTAAGCGCT TCGGCAGCAT GGGTTCCCAC GCCGAAGAGT ATGACCATGA TGATGCCAGC 
GATGGATATG TGCATGAGTT GCATTGTGAT GATGAGGCAG ACGACCTTGC TCAGTACTTG 
GATCACCTCC TGGCTCACTC TGCTTCTAAG AAGCAGAAGT CCAGCGCTGA AGGTGTGGGC 
CGATTCAAAG CTGTTGCTAC AAGAACCAGG GAGATGGTCT CTTTGATGAA CAAGGCTCAG 
GATCTTAACA TCCACAACAT TGGCATGTAC CTTCCCAACA AGCTGTTTCG TACTCGTCAT 
CCATCGCTCA ATCTGAACCT TCCAGACAGT ACTGTCCACG AAGAAGCTCA GGCACCAGAG 
GTGTCCATCA CCACAGAAAG TAGCCTCCCC AGAGATGCCA GCGGCGCCAT CGACTACAGT 
GCTTTGGTCC AGCTGCTGAA AGACAGTGGG AACATCCAGG ACCAGGCAGA TATTCTCTAC 
ATCCTCTTCA AAGACAAGGG GATGGACTGG GACACACACC TTCATGGGAG GGGCTCCACT 
GTCCGGTCTC TGCTCAGCAA CCTTTACGAG AAGGCTGGTG ACCTCAAACA CTGGGGCCTG 
ATCCGGATGA TCTCTGGGAT GCTGAAGAAG AAGGTGGAAG AGCTCGACTC GGCCTGCTCT 
GACCTGCTGG CTCACCAGAA GCACCTGACA GTAGGTCTGC CTCCAGAACC CAGGGAGAAA 
ACCATCACGG CGCCCATCCC TCCAGACCAG CTGGCTGCCC TCATCGATGA GGCCAGCGAT 
GGCAACATCA GTGTTGCCAT CCTCACACAG GAGATCATGG TGTTCCTGGC CATGAGCATC 
CGCACGCAGC CCAGTCTGTT CAGCGAGATG TTCAGGCTCC GGATCGGCCT CATCATTCAA 
GTCATGGCCA CAGAACTGGC TCAGTCCCTC AACTGCTCTG GTGACGAGGC CACAGAGAGT 
CTGATGAGTC TGAGTCCGTC AGAGCTGAAG AACCTGCTCC ACCACATCCT CAGTGGGAAA 
GAGTTTGGCG TGGAGCGCAG CGTCAGAGAG GGAGATGATG GCATCAGTCC TGCCATCTCC 
ATCCACCACC TGGGGAACGT TGGCGCCACC AAGAGTGAGA GAGCGGGCAT CAGCAAGCTG 
AAGAGCGACA TAAAGATGGG CGTTCAAAGT CCGTCGCTGG ATATGGAAAA TGTGGAATCG 
GGGAGGTACC GGCTGCCGTC CATAGAGTCT CTGGACATTT CGGACAGCAT TCCTACCATC 
AAGGACACCA GACATGGACA GTGGCTGCGC AGGAGACGGC TGGACGGCGC GCTGAACCGA 
GTTCCTGTGG GCTTCTACCA GAAAGTGTGG AGGATCCTGC AGAAGTGCCA CGGTCTGTCC 
ATCGAGGGCT TTGTTCTTCC ATCATCCACC ACCAGAGAGA TGACCCCCGC AGAAATCAAG 
TTCTCAGTTC ACGTGGAAAC CGTTCTGAAC CGCGTCCCCC AGCCCGAGTA CCGGCAGCTG 
CTGGTGGAAG CCATTCTGGT CCTCACCATG CTGGCCGACG TGGACATCCC AAGCATCGGC 
TCCATCATCC ATGTGGAGAA GATCGTCCAT TTGGCCAACG ATATGTTCTA CAAAGATCAG 
AAGGACCTGG GGGCCGAGGG GCACATCCTA GAAAAAGATC CGTCCACGGG AGTGTGCCGA 
CTCCTGTATG ACAGCGCCCC CAGTGGTCGC TTTGGAAGCA TGACCTACCT GACCAAGGCG 
GTGGCGGCTT ATGTACAGGA CTTCCTGCCT AGCGGGGCGT GTGCCGTGCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phka1 ( XM_011487671.3 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_011485973.1 Oryzias latipes phosphorylase kinase regulatory subunit alpha 1 (phka1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OJa32967
Clone ID Related Accession (Same CDS sequence)	XM_011487671.2 , XM_011487671.3
Accession Version	XM_011487671.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3654bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-02-14
Organism	Oryzias latipes(Japanese medaka)
Product	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019876.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 15, 2018 this sequence version replaced XM_011487671.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGCC GAAGCAACTC CGGCGTGAAG CTGGACAACT ATGCCCGGAT CGTCCAGCAG 
ACCATCCTGC AGCACCAGGA TCCAGTAACG GGTCTCCTTC CGGGCAGCTT GGAGCAGCCA 
GATGCATGGG TGAGGGACAA CGTCTACAGC ATCGTCTCAG TGTGGGCCCT CAGCCTGGCC 
TACAGGAAAA ATGCCGACCG GGACGAGGAC AAGGCCAAGG CCTACGAGCT GGAGCAGAGT 
GTGGTGAAGC TCATGAGAGG TGTCCTCCAG TGCATTATGA GACAGCTAGA CAAAGTGGAG 
AAGTTCAAAT ACAGCCGAAG TACCAAGGAC TCACTTCACG CCAAGTACAA CCCACGCACC 
TGTGCTACAG TGGTCGGGGA CCACCAGTGG GGTCACCTGC AGGTGGACGC AACCTCGCTC 
TTCCTGCTCT TCCTCGCTCA GATGACGGCA TCCGGTCTGC ACATCATCTA CACGCAGGAT 
GAGGTGGACG TCGTTCAGAA CCTCATGTTC TACATCGAGG CAGCTTACAA AGTGGCTGAT 
TATGGGATGT GGGAGAGGGG GGACAAAACC AACCAGGGCA TCACAGAGAT CAATGCCAGC 
TCCATCGGCA TGGCCAAGGC GGCTCTGGAG GCCCTGGACG AACTCAACCT GTTTGGAGCT 
AAGGGAGGAC CGGGATCTGT GGTCCACGCT CTGGCTGATG ACATCCAGCA CTGCCAGTCC 
ATCCTCACAT CCATGTTACC CAAAGCTTCC ATCTCTAAAG AGGTGGATGC CGGAGTTTTG 
GCTATTCTTA CCTACCCCGC CTTCGCCGTG GAGGACATGA GCATCGTCAC CATGACCAAG 
GAGGAGATCA TCTCCAAGCT GCAGGGTCGG TACGGCTGTT GCAGGTTTCT CAGGGACGGA 
CATAAAACAC CCAAAGAGGA TCCCAACCGC CTGTACTACG AGTCTGCAGA GCTGAAGCTG 
TTCGAGAACA TCGAGTGTGA GTGGCCTCTC TTCTGGACGT ACCTCATTCT GGACGGCATA 
TTCAGCAACA GTCATGAACA GGTGCAGGAG TACCAGGAGG CGCTGGAGGG CATCCTCATC 
AAGCAGAAGG ATGGCATACG ACTGTTTCCA GAGCTCTACA GCGTTCCTCC AGATAAGGTG 
GAAGAGGAAT ACATGAACCC CCACACAGTG GAGAGGATCC CGATGGGGAA ATGTCCCCTG 
AAATGGGGCC AGTCCCTTTA TGTTCTGGGG AGACTCTTAT CCGAGGGGTT TCTTGCTCCT 
GGTGAGATTG ACCCTCTTAA CCGCCGTTTC TCCACCGTGC CCAAGCCGGA CGTGGTCGTT 
CAGGTTTCCA TTTTGGCTGA AACAGATGAG ATTAAGGAGC TTTTGCTGAA GAACGGCTTC 
GAGGTGGAAA CCATCGCAGA CATCCACCCC TTTCATGTGC AGCCCTCCAG AGTCCTCAGC 
CACATCTATG CTAGGCTCGG ACGTAACCCA AGGCTGGGCC TGACGGGACG GCCCTACAGG 
AGAATCGGAG TTCTGGGAAC CTCAAAGTTC TACATCATCA GAAACACCAT CTTCACATTC 
ACTCCACAGT TCATCGACCA CCAGCAGTTC TACCTGGCTC TGGACAACAA AATGATCGTA 
GAGATGTTGA GGACAGAAAT CGCCTACCTG TCCTCCAGGT GGAGGATAAC CGGACGGCCG 
ACGGTCACCT TCCCCGTCTC TCAAACCATG CTGACTGAAG ACCGCACAGA TCTGGACCCG 
GCTGTCCTGG CGACGCTGAA GAAGCTACAG GATGGGTATT TTGGAGGAGC CAGGATCCAG 
ACGGGAAAGC TGTCAGAGTT CCTGACCACC TCCTGCTGCG CTCACCTGAG CTTCCTGGAT 
GGTAAGCGCT TCGGCAGCAT GGGTTCCCAC GCCGAAGAGT ATGACCATGA TGATGCCAGC 
GATGGATATG TGCATGAGTT GCATTGTGAT GATGAGGCAG ACGACCTTGC TCAGTACTTG 
GATCACCTCC TGGCTCACTC TGCTTCTAAG AAGCAGAAGT CCAGCGCTGA AGGTGTGGGC 
CGATTCAAAG CTGTTGCTAC AAGAACCAGG GAGATGGTCT CTTTGATGAA CAAGGCTCAG 
GATCTTAACA TCCACAACAT TGGCATGTAC CTTCCCAACA AGCTGTTTCG TACTCGTCAT 
CCATCGCTCA ATCTGAACCT TCCAGACAGT ACTGTCCACG AAGAAGCTCA GGCACCAGAG 
GTGTCCATCA CCACAGAAAG TAGCCTCCCC AGAGATGCCA GCGGCGCCAT CGACTACAGT 
GCTTTGGTCC AGCTGCTGAA AGACAGTGGG AACATCCAGG ACCAGGCAGA TATTCTCTAC 
ATCCTCTTCA AAGACAAGGG GATGGACTGG GACACACACC TTCATGGGAG GGGCTCCACT 
GTCCGGTCTC TGCTCAGCAA CCTTTACGAG AAGGCTGGTG ACCTCAAACA CTGGGGCCTG 
ATCCGGATGA TCTCTGGGAT GCTGAAGAAG AAGGTGGAAG AGCTCGACTC GGCCTGCTCT 
GACCTGCTGG CTCACCAGAA GCACCTGACA GTAGGTCTGC CTCCAGAACC CAGGGAGAAA 
ACCATCACGG CGCCCATCCC TCCAGACCAG CTGGCTGCCC TCATCGATGA GGCCAGCGAT 
GGCAACATCA GTGTTGCCAT CCTCACACAG GAGATCATGG TGTTCCTGGC CATGAGCATC 
CGCACGCAGC CCAGTCTGTT CAGCGAGATG TTCAGGCTCC GGATCGGCCT CATCATTCAA 
GTCATGGCCA CAGAACTGGC TCAGTCCCTC AACTGCTCTG GTGACGAGGC CACAGAGAGT 
CTGATGAGTC TGAGTCCGTC AGAGCTGAAG AACCTGCTCC ACCACATCCT CAGTGGGAAA 
GAGTTTGGCG TGGAGCGCAG CGTCAGAGAG GGAGATGATG GCATCAGTCC TGCCATCTCC 
ATCCACCACC TGGGGAACGT TGGCGCCACC AAGAGTGAGA GAGCGGGCAT CAGCAAGCTG 
AAGAGCGACA TAAAGATGGG CGTTCAAAGT CCGTCGCTGG ATATGGAAAA TGTGGAATCG 
GGGAGGTACC GGCTGCCGTC CATAGAGTCT CTGGACATTT CGGACAGCAT TCCTACCATC 
AAGGACACCA GACATGGACA GTGGCTGCGC AGGAGACGGC TGGACGGCGC GCTGAACCGA 
GTTCCTGTGG GCTTCTACCA GAAAGTGTGG AGGATCCTGC AGAAGTGCCA CGGTCTGTCC 
ATCGAGGGCT TTGTTCTTCC ATCATCCACC ACCAGAGAGA TGACCCCCGC AGAAATCAAG 
TTCTCAGTTC ACGTGGAAAC CGTTCTGAAC CGCGTCCCCC AGCCCGAGTA CCGGCAGCTG 
CTGGTGGAAG CCATTCTGGT CCTCACCATG CTGGCCGACG TGGACATCCC AAGCATCGGC 
TCCATCATCC ATGTGGAGAA GATCGTCCAT TTGGCCAACG ATATGTTCTA CAAAGATCAG 
AAGGACCTGG GGGCCGAGGG GCACATCCTA GAAAAAGATC CGTCCACGGG AGTGTGCCGA 
CTCCTGTATG ACAGCGCCCC CAGTGGTCGC TTTGGAAGCA TGACCTACCT GACCAAGGCG 
GTGGCGGCTT ATGTACAGGA CTTCCTGCCT AGCGGGGCGT GTGCCGTGCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011485973.1
CDS	188..3841
Translation	MRSRSNSGVKLDNYARIVQQTILQHQDPVTGLLPGSLEQPDAWVRDNVYSIVSVWALSLAYRKNADRDEDKAKAYELEQSVVKLMRGVLQCIMRQLDKVEKFKYSRSTKDSLHAKYNPRTCATVVGDHQWGHLQVDATSLFLLFLAQMTASGLHIIYTQDEVDVVQNLMFYIEAAYKVADYGMWERGDKTNQGITEINASSIGMAKAALEALDELNLFGAKGGPGSVVHALADDIQHCQSILTSMLPKASISKEVDAGVLAILTYPAFAVEDMSIVTMTKEEIISKLQGRYGCCRFLRDGHKTPKEDPNRLYYESAELKLFENIECEWPLFWTYLILDGIFSNSHEQVQEYQEALEGILIKQKDGIRLFPELYSVPPDKVEEEYMNPHTVERIPMGKCPLKWGQSLYVLGRLLSEGFLAPGEIDPLNRRFSTVPKPDVVVQVSILAETDEIKELLLKNGFEVETIADIHPFHVQPSRVLSHIYARLGRNPRLGLTGRPYRRIGVLGTSKFYIIRNTIFTFTPQFIDHQQFYLALDNKMIVEMLRTEIAYLSSRWRITGRPTVTFPVSQTMLTEDRTDLDPAVLATLKKLQDGYFGGARIQTGKLSEFLTTSCCAHLSFLDGKRFGSMGSHAEEYDHDDASDGYVHELHCDDEADDLAQYLDHLLAHSASKKQKSSAEGVGRFKAVATRTREMVSLMNKAQDLNIHNIGMYLPNKLFRTRHPSLNLNLPDSTVHEEAQAPEVSITTESSLPRDASGAIDYSALVQLLKDSGNIQDQADILYILFKDKGMDWDTHLHGRGSTVRSLLSNLYEKAGDLKHWGLIRMISGMLKKKVEELDSACSDLLAHQKHLTVGLPPEPREKTITAPIPPDQLAALIDEASDGNISVAILTQEIMVFLAMSIRTQPSLFSEMFRLRIGLIIQVMATELAQSLNCSGDEATESLMSLSPSELKNLLHHILSGKEFGVERSVREGDDGISPAISIHHLGNVGATKSERAGISKLKSDIKMGVQSPSLDMENVESGRYRLPSIESLDISDSIPTIKDTRHGQWLRRRRLDGALNRVPVGFYQKVWRILQKCHGLSIEGFVLPSSTTREMTPAEIKFSVHVETVLNRVPQPEYRQLLVEAILVLTMLADVDIPSIGSIIHVEKIVHLANDMFYKDQKDLGAEGHILEKDPSTGVCRLLYDSAPSGRFGSMTYLTKAVAAYVQDFLPSGACAVQ

Target ORF information:

RefSeq Version	XM_011487671.3
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes phosphorylase kinase regulatory subunit alpha 1 (phka1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011487671.3

ORF Insert Sequence:

ATGAGGAGCC GAAGCAACTC CGGCGTGAAG CTGGACAACT ATGCCCGGAT CGTCCAGCAG 
ACCATCCTGC AGCACCAGGA TCCAGTAACG GGTCTCCTTC CGGGCAGCTT GGAGCAGCCA 
GATGCATGGG TGAGGGACAA CGTCTACAGC ATCGTCTCAG TGTGGGCCCT CAGCCTGGCC 
TACAGGAAAA ATGCCGACCG GGACGAGGAC AAGGCCAAGG CCTACGAGCT GGAGCAGAGT 
GTGGTGAAGC TCATGAGAGG TGTCCTCCAG TGCATTATGA GACAGCTAGA CAAAGTGGAG 
AAGTTCAAAT ACAGCCGAAG TACCAAGGAC TCACTTCACG CCAAGTACAA CCCACGCACC 
TGTGCTACAG TGGTCGGGGA CCACCAGTGG GGTCACCTGC AGGTGGACGC AACCTCGCTC 
TTCCTGCTCT TCCTCGCTCA GATGACGGCA TCCGGTCTGC ACATCATCTA CACGCAGGAT 
GAGGTGGACG TCGTTCAGAA CCTCATGTTC TACATCGAGG CAGCTTACAA AGTGGCTGAT 
TATGGGATGT GGGAGAGGGG GGACAAAACC AACCAGGGCA TCACAGAGAT CAATGCCAGC 
TCCATCGGCA TGGCCAAGGC GGCTCTGGAG GCCCTGGACG AACTCAACCT GTTTGGAGCT 
AAGGGAGGAC CGGGATCTGT GGTCCACGCT CTGGCTGATG ACATCCAGCA CTGCCAGTCC 
ATCCTCACAT CCATGTTACC CAAAGCTTCC ATCTCTAAAG AGGTGGATGC CGGAGTTTTG 
GCTATTCTTA CCTACCCCGC CTTCGCCGTG GAGGACATGA GCATCGTCAC CATGACCAAG 
GAGGAGATCA TCTCCAAGCT GCAGGGTCGG TACGGCTGTT GCAGGTTTCT CAGGGACGGA 
CATAAAACAC CCAAAGAGGA TCCCAACCGC CTGTACTACG AGTCTGCAGA GCTGAAGCTG 
TTCGAGAACA TCGAGTGTGA GTGGCCTCTC TTCTGGACGT ACCTCATTCT GGACGGCATA 
TTCAGCAACA GTCATGAACA GGTGCAGGAG TACCAGGAGG CGCTGGAGGG CATCCTCATC 
AAGCAGAAGG ATGGCATACG ACTGTTTCCA GAGCTCTACA GCGTTCCTCC AGATAAGGTG 
GAAGAGGAAT ACATGAACCC CCACACAGTG GAGAGGATCC CGATGGGGAA ATGTCCCCTG 
AAATGGGGCC AGTCCCTTTA TGTTCTGGGG AGACTCTTAT CCGAGGGGTT TCTTGCTCCT 
GGTGAGATTG ACCCTCTTAA CCGCCGTTTC TCCACCGTGC CCAAGCCGGA CGTGGTCGTT 
CAGGTTTCCA TTTTGGCTGA AACAGATGAG ATTAAGGAGC TTTTGCTGAA GAACGGCTTC 
GAGGTGGAAA CCATCGCAGA CATCCACCCC TTTCATGTGC AGCCCTCCAG AGTCCTCAGC 
CACATCTATG CTAGGCTCGG ACGTAACCCA AGGCTGGGCC TGACGGGACG GCCCTACAGG 
AGAATCGGAG TTCTGGGAAC CTCAAAGTTC TACATCATCA GAAACACCAT CTTCACATTC 
ACTCCACAGT TCATCGACCA CCAGCAGTTC TACCTGGCTC TGGACAACAA AATGATCGTA 
GAGATGTTGA GGACAGAAAT CGCCTACCTG TCCTCCAGGT GGAGGATAAC CGGACGGCCG 
ACGGTCACCT TCCCCGTCTC TCAAACCATG CTGACTGAAG ACCGCACAGA TCTGGACCCG 
GCTGTCCTGG CGACGCTGAA GAAGCTACAG GATGGGTATT TTGGAGGAGC CAGGATCCAG 
ACGGGAAAGC TGTCAGAGTT CCTGACCACC TCCTGCTGCG CTCACCTGAG CTTCCTGGAT 
GGTAAGCGCT TCGGCAGCAT GGGTTCCCAC GCCGAAGAGT ATGACCATGA TGATGCCAGC 
GATGGATATG TGCATGAGTT GCATTGTGAT GATGAGGCAG ACGACCTTGC TCAGTACTTG 
GATCACCTCC TGGCTCACTC TGCTTCTAAG AAGCAGAAGT CCAGCGCTGA AGGTGTGGGC 
CGATTCAAAG CTGTTGCTAC AAGAACCAGG GAGATGGTCT CTTTGATGAA CAAGGCTCAG 
GATCTTAACA TCCACAACAT TGGCATGTAC CTTCCCAACA AGCTGTTTCG TACTCGTCAT 
CCATCGCTCA ATCTGAACCT TCCAGACAGT ACTGTCCACG AAGAAGCTCA GGCACCAGAG 
GTGTCCATCA CCACAGAAAG TAGCCTCCCC AGAGATGCCA GCGGCGCCAT CGACTACAGT 
GCTTTGGTCC AGCTGCTGAA AGACAGTGGG AACATCCAGG ACCAGGCAGA TATTCTCTAC 
ATCCTCTTCA AAGACAAGGG GATGGACTGG GACACACACC TTCATGGGAG GGGCTCCACT 
GTCCGGTCTC TGCTCAGCAA CCTTTACGAG AAGGCTGGTG ACCTCAAACA CTGGGGCCTG 
ATCCGGATGA TCTCTGGGAT GCTGAAGAAG AAGGTGGAAG AGCTCGACTC GGCCTGCTCT 
GACCTGCTGG CTCACCAGAA GCACCTGACA GTAGGTCTGC CTCCAGAACC CAGGGAGAAA 
ACCATCACGG CGCCCATCCC TCCAGACCAG CTGGCTGCCC TCATCGATGA GGCCAGCGAT 
GGCAACATCA GTGTTGCCAT CCTCACACAG GAGATCATGG TGTTCCTGGC CATGAGCATC 
CGCACGCAGC CCAGTCTGTT CAGCGAGATG TTCAGGCTCC GGATCGGCCT CATCATTCAA 
GTCATGGCCA CAGAACTGGC TCAGTCCCTC AACTGCTCTG GTGACGAGGC CACAGAGAGT 
CTGATGAGTC TGAGTCCGTC AGAGCTGAAG AACCTGCTCC ACCACATCCT CAGTGGGAAA 
GAGTTTGGCG TGGAGCGCAG CGTCAGAGAG GGAGATGATG GCATCAGTCC TGCCATCTCC 
ATCCACCACC TGGGGAACGT TGGCGCCACC AAGAGTGAGA GAGCGGGCAT CAGCAAGCTG 
AAGAGCGACA TAAAGATGGG CGTTCAAAGT CCGTCGCTGG ATATGGAAAA TGTGGAATCG 
GGGAGGTACC GGCTGCCGTC CATAGAGTCT CTGGACATTT CGGACAGCAT TCCTACCATC 
AAGGACACCA GACATGGACA GTGGCTGCGC AGGAGACGGC TGGACGGCGC GCTGAACCGA 
GTTCCTGTGG GCTTCTACCA GAAAGTGTGG AGGATCCTGC AGAAGTGCCA CGGTCTGTCC 
ATCGAGGGCT TTGTTCTTCC ATCATCCACC ACCAGAGAGA TGACCCCCGC AGAAATCAAG 
TTCTCAGTTC ACGTGGAAAC CGTTCTGAAC CGCGTCCCCC AGCCCGAGTA CCGGCAGCTG 
CTGGTGGAAG CCATTCTGGT CCTCACCATG CTGGCCGACG TGGACATCCC AAGCATCGGC 
TCCATCATCC ATGTGGAGAA GATCGTCCAT TTGGCCAACG ATATGTTCTA CAAAGATCAG 
AAGGACCTGG GGGCCGAGGG GCACATCCTA GAAAAAGATC CGTCCACGGG AGTGTGCCGA 
CTCCTGTATG ACAGCGCCCC CAGTGGTCGC TTTGGAAGCA TGACCTACCT GACCAAGGCG 
GTGGCGGCTT ATGTACAGGA CTTCCTGCCT AGCGGGGCGT GTGCCGTGCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phka1 ( XM_011487672.2 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_011485974.1 Oryzias latipes phosphorylase kinase regulatory subunit alpha 1 (phka1), transcript variant X2, mRNA.

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CloneID	OJa32968
Clone ID Related Accession (Same CDS sequence)	XM_011487672.2 , XM_011487672.3
Accession Version	XM_011487672.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3561bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-06
Organism	Oryzias latipes(Japanese medaka)
Product	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019876.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 7, 2017 this sequence version replaced XM_011487672.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryzias latipes Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGCC GAAGCAACTC CGGCGTGAAG CTGGACAACT ATGCCCGGAT CGTCCAGCAG 
ACCATCCTGC AGCACCAGGA TCCAGTAACG GGTCTCCTTC CGGGCAGCTT GGAGCAGCCA 
GATGCATGGG TGAGGGACAA CGTCTACAGC ATCGTCTCAG TGTGGGCCCT CAGCCTGGCC 
TACAGGAAAA ATGCCGACCG GGACGAGGAC AAGGCCAAGG CCTACGAGCT GGAGCAGAGT 
GTGGTGAAGC TCATGAGAGG TGTCCTCCAG TGCATTATGA GACAGCTAGA CAAAGTGGAG 
AAGTTCAAAT ACAGCCGAAG TACCAAGGAC TCACTTCACG CCAAGTACAA CCCACGCACC 
TGTGCTACAG TGGTCGGGGA CCACCAGTGG GGTCACCTGC AGGTGGACGC AACCTCGCTC 
TTCCTGCTCT TCCTCGCTCA GATGACGGCA TCCGGTCTGC ACATCATCTA CACGCAGGAT 
GAGGTGGACG TCGTTCAGAA CCTCATGTTC TACATCGAGG CAGCTTACAA AGTGGCTGAT 
TATGGGATGT GGGAGAGGGG GGACAAAACC AACCAGGGCA TCACAGAGAT CAATGCCAGC 
TCCATCGGCA TGGCCAAGGC GGCTCTGGAG GCCCTGGACG AACTCAACCT GTTTGGAGCT 
AAGGGAGGAC CGGGATCTGT GGTCCACGCT CTGGCTGATG ACATCCAGCA CTGCCAGTCC 
ATCCTCACAT CCATGTTACC CAAAGCTTCC ATCTCTAAAG AGGTGGATGC CGGAGTTTTG 
GCTATTCTTA CCTACCCCGC CTTCGCCGTG GAGGACATGA GCATCGTCAC CATGACCAAG 
GAGGAGATCA TCTCCAAGCT GCAGGGTCGG TACGGCTGTT GCAGGTTTCT CAGGGACGGA 
CATAAAACAC CCAAAGAGGA TCCCAACCGC CTGTACTACG AGTCTGCAGA GCTGAAGCTG 
TTCGAGAACA TCGAGTGTGA GTGGCCTCTC TTCTGGACGT ACCTCATTCT GGACGGCATA 
TTCAGCAACA GTCATGAACA GGTGCAGGAG TACCAGGAGG CGCTGGAGGG CATCCTCATC 
AAGCAGAAGG ATGGCATACG ACTGTTTCCA GAGCTCTACA GCGTTCCTCC AGATAAGGTG 
GAAGAGGAAT ACATGAACCC CCACACAGTG GAGAGGATCC CGATGGGGAA ATGTCCCCTG 
AAATGGGGCC AGTCCCTTTA TGTTCTGGGG AGACTCTTAT CCGAGGGGTT TCTTGCTCCT 
GGTGAGATTG ACCCTCTTAA CCGCCGTTTC TCCACCGTGC CCAAGCCGGA CGTGGTCGTT 
CAGGTTTCCA TTTTGGCTGA AACAGATGAG ATTAAGGAGC TTTTGCTGAA GAACGGCTTC 
GAGGTGGAAA CCATCGCAGA CATCCACCCC TTTCATGTGC AGCCCTCCAG AGTCCTCAGC 
CACATCTATG CTAGGCTCGG ACGTAACCCA AGGCTGGGCC TGACGGGACG GCCCTACAGG 
AGAATCGGAG TTCTGGGAAC CTCAAAGTTC TACATCATCA GAAACACCAT CTTCACATTC 
ACTCCACAGT TCATCGACCA CCAGCAGTTC TACCTGGCTC TGGACAACAA AATGATCGTA 
GAGATGTTGA GGACAGAAAT CGCCTACCTG TCCTCCAGGT GGAGGATAAC CGGACGGCCG 
ACGGTCACCT TCCCCGTCTC TCAAACCATG CTGACTGAAG ACCGCACAGA TCTGGACCCG 
GCTGTCCTGG CGACGCTGAA GAAGCTACAG GATGGGTATT TTGGAGGAGC CAGGATCCAG 
ACGGGAAAGC TGTCAGAGTT CCTGACCACC TCCTGCTGCG CTCACCTGAG CTTCCTGGAT 
GAGGCAGACG ACCTTGCTCA GTACTTGGAT CACCTCCTGG CTCACTCTGC TTCTAAGAAG 
CAGAAGTCCA GCGCTGAAGG TGTGGGCCGA TTCAAAGCTG TTGCTACAAG AACCAGGGAG 
ATGGTCTCTT TGATGAACAA GGCTCAGGAT CTTAACATCC ACAACATTGG CATGTACCTT 
CCCAACAAGC TGTTTCGTAC TCGTCATCCA TCGCTCAATC TGAACCTTCC AGACAGTACT 
GTCCACGAAG AAGCTCAGGC ACCAGAGGTG TCCATCACCA CAGAAAGTAG CCTCCCCAGA 
GATGCCAGCG GCGCCATCGA CTACAGTGCT TTGGTCCAGC TGCTGAAAGA CAGTGGGAAC 
ATCCAGGACC AGGCAGATAT TCTCTACATC CTCTTCAAAG ACAAGGGGAT GGACTGGGAC 
ACACACCTTC ATGGGAGGGG CTCCACTGTC CGGTCTCTGC TCAGCAACCT TTACGAGAAG 
GCTGGTGACC TCAAACACTG GGGCCTGATC CGGATGATCT CTGGGATGCT GAAGAAGAAG 
GTGGAAGAGC TCGACTCGGC CTGCTCTGAC CTGCTGGCTC ACCAGAAGCA CCTGACAGTA 
GGTCTGCCTC CAGAACCCAG GGAGAAAACC ATCACGGCGC CCATCCCTCC AGACCAGCTG 
GCTGCCCTCA TCGATGAGGC CAGCGATGGC AACATCAGTG TTGCCATCCT CACACAGGAG 
ATCATGGTGT TCCTGGCCAT GAGCATCCGC ACGCAGCCCA GTCTGTTCAG CGAGATGTTC 
AGGCTCCGGA TCGGCCTCAT CATTCAAGTC ATGGCCACAG AACTGGCTCA GTCCCTCAAC 
TGCTCTGGTG ACGAGGCCAC AGAGAGTCTG ATGAGTCTGA GTCCGTCAGA GCTGAAGAAC 
CTGCTCCACC ACATCCTCAG TGGGAAAGAG TTTGGCGTGG AGCGCAGCGT CAGAGAGGGA 
GATGATGGCA TCAGTCCTGC CATCTCCATC CACCACCTGG GGAACGTTGG CGCCACCAAG 
AGTGAGAGAG CGGGCATCAG CAAGCTGAAG AGCGACATAA AGATGGGCGT TCAAAGTCCG 
TCGCTGGATA TGGAAAATGT GGAATCGGGG AGGTACCGGC TGCCGTCCAT AGAGTCTCTG 
GACATTTCGG ACAGCATTCC TACCATCAAG GACACCAGAC ATGGACAGTG GCTGCGCAGG 
AGACGGCTGG ACGGCGCGCT GAACCGAGTT CCTGTGGGCT TCTACCAGAA AGTGTGGAGG 
ATCCTGCAGA AGTGCCACGG TCTGTCCATC GAGGGCTTTG TTCTTCCATC ATCCACCACC 
AGAGAGATGA CCCCCGCAGA AATCAAGTTC TCAGTTCACG TGGAAACCGT TCTGAACCGC 
GTCCCCCAGC CCGAGTACCG GCAGCTGCTG GTGGAAGCCA TTCTGGTCCT CACCATGCTG 
GCCGACGTGG ACATCCCAAG CATCGGCTCC ATCATCCATG TGGAGAAGAT CGTCCATTTG 
GCCAACGATA TGTTCTACAA AGATCAGAAG GACCTGGGGG CCGAGGGGCA CATCCTAGAA 
AAAGATCCGT CCACGGGAGT GTGCCGACTC CTGTATGACA GCGCCCCCAG TGGTCGCTTT 
GGAAGCATGA CCTACCTGAC CAAGGCGGTG GCGGCTTATG TACAGGACTT CCTGCCTAGC 
GGGGCGTGTG CCGTGCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011485974.1
CDS	192..3752
Translation	MRSRSNSGVKLDNYARIVQQTILQHQDPVTGLLPGSLEQPDAWVRDNVYSIVSVWALSLAYRKNADRDEDKAKAYELEQSVVKLMRGVLQCIMRQLDKVEKFKYSRSTKDSLHAKYNPRTCATVVGDHQWGHLQVDATSLFLLFLAQMTASGLHIIYTQDEVDVVQNLMFYIEAAYKVADYGMWERGDKTNQGITEINASSIGMAKAALEALDELNLFGAKGGPGSVVHALADDIQHCQSILTSMLPKASISKEVDAGVLAILTYPAFAVEDMSIVTMTKEEIISKLQGRYGCCRFLRDGHKTPKEDPNRLYYESAELKLFENIECEWPLFWTYLILDGIFSNSHEQVQEYQEALEGILIKQKDGIRLFPELYSVPPDKVEEEYMNPHTVERIPMGKCPLKWGQSLYVLGRLLSEGFLAPGEIDPLNRRFSTVPKPDVVVQVSILAETDEIKELLLKNGFEVETIADIHPFHVQPSRVLSHIYARLGRNPRLGLTGRPYRRIGVLGTSKFYIIRNTIFTFTPQFIDHQQFYLALDNKMIVEMLRTEIAYLSSRWRITGRPTVTFPVSQTMLTEDRTDLDPAVLATLKKLQDGYFGGARIQTGKLSEFLTTSCCAHLSFLDEADDLAQYLDHLLAHSASKKQKSSAEGVGRFKAVATRTREMVSLMNKAQDLNIHNIGMYLPNKLFRTRHPSLNLNLPDSTVHEEAQAPEVSITTESSLPRDASGAIDYSALVQLLKDSGNIQDQADILYILFKDKGMDWDTHLHGRGSTVRSLLSNLYEKAGDLKHWGLIRMISGMLKKKVEELDSACSDLLAHQKHLTVGLPPEPREKTITAPIPPDQLAALIDEASDGNISVAILTQEIMVFLAMSIRTQPSLFSEMFRLRIGLIIQVMATELAQSLNCSGDEATESLMSLSPSELKNLLHHILSGKEFGVERSVREGDDGISPAISIHHLGNVGATKSERAGISKLKSDIKMGVQSPSLDMENVESGRYRLPSIESLDISDSIPTIKDTRHGQWLRRRRLDGALNRVPVGFYQKVWRILQKCHGLSIEGFVLPSSTTREMTPAEIKFSVHVETVLNRVPQPEYRQLLVEAILVLTMLADVDIPSIGSIIHVEKIVHLANDMFYKDQKDLGAEGHILEKDPSTGVCRLLYDSAPSGRFGSMTYLTKAVAAYVQDFLPSGACAVQ

Target ORF information:

RefSeq Version	XM_011487672.2
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes phosphorylase kinase regulatory subunit alpha 1 (phka1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011487672.2

ORF Insert Sequence:

ATGAGGAGCC GAAGCAACTC CGGCGTGAAG CTGGACAACT ATGCCCGGAT CGTCCAGCAG 
ACCATCCTGC AGCACCAGGA TCCAGTAACG GGTCTCCTTC CGGGCAGCTT GGAGCAGCCA 
GATGCATGGG TGAGGGACAA CGTCTACAGC ATCGTCTCAG TGTGGGCCCT CAGCCTGGCC 
TACAGGAAAA ATGCCGACCG GGACGAGGAC AAGGCCAAGG CCTACGAGCT GGAGCAGAGT 
GTGGTGAAGC TCATGAGAGG TGTCCTCCAG TGCATTATGA GACAGCTAGA CAAAGTGGAG 
AAGTTCAAAT ACAGCCGAAG TACCAAGGAC TCACTTCACG CCAAGTACAA CCCACGCACC 
TGTGCTACAG TGGTCGGGGA CCACCAGTGG GGTCACCTGC AGGTGGACGC AACCTCGCTC 
TTCCTGCTCT TCCTCGCTCA GATGACGGCA TCCGGTCTGC ACATCATCTA CACGCAGGAT 
GAGGTGGACG TCGTTCAGAA CCTCATGTTC TACATCGAGG CAGCTTACAA AGTGGCTGAT 
TATGGGATGT GGGAGAGGGG GGACAAAACC AACCAGGGCA TCACAGAGAT CAATGCCAGC 
TCCATCGGCA TGGCCAAGGC GGCTCTGGAG GCCCTGGACG AACTCAACCT GTTTGGAGCT 
AAGGGAGGAC CGGGATCTGT GGTCCACGCT CTGGCTGATG ACATCCAGCA CTGCCAGTCC 
ATCCTCACAT CCATGTTACC CAAAGCTTCC ATCTCTAAAG AGGTGGATGC CGGAGTTTTG 
GCTATTCTTA CCTACCCCGC CTTCGCCGTG GAGGACATGA GCATCGTCAC CATGACCAAG 
GAGGAGATCA TCTCCAAGCT GCAGGGTCGG TACGGCTGTT GCAGGTTTCT CAGGGACGGA 
CATAAAACAC CCAAAGAGGA TCCCAACCGC CTGTACTACG AGTCTGCAGA GCTGAAGCTG 
TTCGAGAACA TCGAGTGTGA GTGGCCTCTC TTCTGGACGT ACCTCATTCT GGACGGCATA 
TTCAGCAACA GTCATGAACA GGTGCAGGAG TACCAGGAGG CGCTGGAGGG CATCCTCATC 
AAGCAGAAGG ATGGCATACG ACTGTTTCCA GAGCTCTACA GCGTTCCTCC AGATAAGGTG 
GAAGAGGAAT ACATGAACCC CCACACAGTG GAGAGGATCC CGATGGGGAA ATGTCCCCTG 
AAATGGGGCC AGTCCCTTTA TGTTCTGGGG AGACTCTTAT CCGAGGGGTT TCTTGCTCCT 
GGTGAGATTG ACCCTCTTAA CCGCCGTTTC TCCACCGTGC CCAAGCCGGA CGTGGTCGTT 
CAGGTTTCCA TTTTGGCTGA AACAGATGAG ATTAAGGAGC TTTTGCTGAA GAACGGCTTC 
GAGGTGGAAA CCATCGCAGA CATCCACCCC TTTCATGTGC AGCCCTCCAG AGTCCTCAGC 
CACATCTATG CTAGGCTCGG ACGTAACCCA AGGCTGGGCC TGACGGGACG GCCCTACAGG 
AGAATCGGAG TTCTGGGAAC CTCAAAGTTC TACATCATCA GAAACACCAT CTTCACATTC 
ACTCCACAGT TCATCGACCA CCAGCAGTTC TACCTGGCTC TGGACAACAA AATGATCGTA 
GAGATGTTGA GGACAGAAAT CGCCTACCTG TCCTCCAGGT GGAGGATAAC CGGACGGCCG 
ACGGTCACCT TCCCCGTCTC TCAAACCATG CTGACTGAAG ACCGCACAGA TCTGGACCCG 
GCTGTCCTGG CGACGCTGAA GAAGCTACAG GATGGGTATT TTGGAGGAGC CAGGATCCAG 
ACGGGAAAGC TGTCAGAGTT CCTGACCACC TCCTGCTGCG CTCACCTGAG CTTCCTGGAT 
GAGGCAGACG ACCTTGCTCA GTACTTGGAT CACCTCCTGG CTCACTCTGC TTCTAAGAAG 
CAGAAGTCCA GCGCTGAAGG TGTGGGCCGA TTCAAAGCTG TTGCTACAAG AACCAGGGAG 
ATGGTCTCTT TGATGAACAA GGCTCAGGAT CTTAACATCC ACAACATTGG CATGTACCTT 
CCCAACAAGC TGTTTCGTAC TCGTCATCCA TCGCTCAATC TGAACCTTCC AGACAGTACT 
GTCCACGAAG AAGCTCAGGC ACCAGAGGTG TCCATCACCA CAGAAAGTAG CCTCCCCAGA 
GATGCCAGCG GCGCCATCGA CTACAGTGCT TTGGTCCAGC TGCTGAAAGA CAGTGGGAAC 
ATCCAGGACC AGGCAGATAT TCTCTACATC CTCTTCAAAG ACAAGGGGAT GGACTGGGAC 
ACACACCTTC ATGGGAGGGG CTCCACTGTC CGGTCTCTGC TCAGCAACCT TTACGAGAAG 
GCTGGTGACC TCAAACACTG GGGCCTGATC CGGATGATCT CTGGGATGCT GAAGAAGAAG 
GTGGAAGAGC TCGACTCGGC CTGCTCTGAC CTGCTGGCTC ACCAGAAGCA CCTGACAGTA 
GGTCTGCCTC CAGAACCCAG GGAGAAAACC ATCACGGCGC CCATCCCTCC AGACCAGCTG 
GCTGCCCTCA TCGATGAGGC CAGCGATGGC AACATCAGTG TTGCCATCCT CACACAGGAG 
ATCATGGTGT TCCTGGCCAT GAGCATCCGC ACGCAGCCCA GTCTGTTCAG CGAGATGTTC 
AGGCTCCGGA TCGGCCTCAT CATTCAAGTC ATGGCCACAG AACTGGCTCA GTCCCTCAAC 
TGCTCTGGTG ACGAGGCCAC AGAGAGTCTG ATGAGTCTGA GTCCGTCAGA GCTGAAGAAC 
CTGCTCCACC ACATCCTCAG TGGGAAAGAG TTTGGCGTGG AGCGCAGCGT CAGAGAGGGA 
GATGATGGCA TCAGTCCTGC CATCTCCATC CACCACCTGG GGAACGTTGG CGCCACCAAG 
AGTGAGAGAG CGGGCATCAG CAAGCTGAAG AGCGACATAA AGATGGGCGT TCAAAGTCCG 
TCGCTGGATA TGGAAAATGT GGAATCGGGG AGGTACCGGC TGCCGTCCAT AGAGTCTCTG 
GACATTTCGG ACAGCATTCC TACCATCAAG GACACCAGAC ATGGACAGTG GCTGCGCAGG 
AGACGGCTGG ACGGCGCGCT GAACCGAGTT CCTGTGGGCT TCTACCAGAA AGTGTGGAGG 
ATCCTGCAGA AGTGCCACGG TCTGTCCATC GAGGGCTTTG TTCTTCCATC ATCCACCACC 
AGAGAGATGA CCCCCGCAGA AATCAAGTTC TCAGTTCACG TGGAAACCGT TCTGAACCGC 
GTCCCCCAGC CCGAGTACCG GCAGCTGCTG GTGGAAGCCA TTCTGGTCCT CACCATGCTG 
GCCGACGTGG ACATCCCAAG CATCGGCTCC ATCATCCATG TGGAGAAGAT CGTCCATTTG 
GCCAACGATA TGTTCTACAA AGATCAGAAG GACCTGGGGG CCGAGGGGCA CATCCTAGAA 
AAAGATCCGT CCACGGGAGT GTGCCGACTC CTGTATGACA GCGCCCCCAG TGGTCGCTTT 
GGAAGCATGA CCTACCTGAC CAAGGCGGTG GCGGCTTATG TACAGGACTT CCTGCCTAGC 
GGGGCGTGTG CCGTGCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phka1 ( XM_011487672.3 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_011485974.1 Oryzias latipes phosphorylase kinase regulatory subunit alpha 1 (phka1), transcript variant X2, mRNA.

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CloneID	OJa32968
Clone ID Related Accession (Same CDS sequence)	XM_011487672.2 , XM_011487672.3
Accession Version	XM_011487672.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3561bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-02-14
Organism	Oryzias latipes(Japanese medaka)
Product	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019876.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 15, 2018 this sequence version replaced XM_011487672.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGCC GAAGCAACTC CGGCGTGAAG CTGGACAACT ATGCCCGGAT CGTCCAGCAG 
ACCATCCTGC AGCACCAGGA TCCAGTAACG GGTCTCCTTC CGGGCAGCTT GGAGCAGCCA 
GATGCATGGG TGAGGGACAA CGTCTACAGC ATCGTCTCAG TGTGGGCCCT CAGCCTGGCC 
TACAGGAAAA ATGCCGACCG GGACGAGGAC AAGGCCAAGG CCTACGAGCT GGAGCAGAGT 
GTGGTGAAGC TCATGAGAGG TGTCCTCCAG TGCATTATGA GACAGCTAGA CAAAGTGGAG 
AAGTTCAAAT ACAGCCGAAG TACCAAGGAC TCACTTCACG CCAAGTACAA CCCACGCACC 
TGTGCTACAG TGGTCGGGGA CCACCAGTGG GGTCACCTGC AGGTGGACGC AACCTCGCTC 
TTCCTGCTCT TCCTCGCTCA GATGACGGCA TCCGGTCTGC ACATCATCTA CACGCAGGAT 
GAGGTGGACG TCGTTCAGAA CCTCATGTTC TACATCGAGG CAGCTTACAA AGTGGCTGAT 
TATGGGATGT GGGAGAGGGG GGACAAAACC AACCAGGGCA TCACAGAGAT CAATGCCAGC 
TCCATCGGCA TGGCCAAGGC GGCTCTGGAG GCCCTGGACG AACTCAACCT GTTTGGAGCT 
AAGGGAGGAC CGGGATCTGT GGTCCACGCT CTGGCTGATG ACATCCAGCA CTGCCAGTCC 
ATCCTCACAT CCATGTTACC CAAAGCTTCC ATCTCTAAAG AGGTGGATGC CGGAGTTTTG 
GCTATTCTTA CCTACCCCGC CTTCGCCGTG GAGGACATGA GCATCGTCAC CATGACCAAG 
GAGGAGATCA TCTCCAAGCT GCAGGGTCGG TACGGCTGTT GCAGGTTTCT CAGGGACGGA 
CATAAAACAC CCAAAGAGGA TCCCAACCGC CTGTACTACG AGTCTGCAGA GCTGAAGCTG 
TTCGAGAACA TCGAGTGTGA GTGGCCTCTC TTCTGGACGT ACCTCATTCT GGACGGCATA 
TTCAGCAACA GTCATGAACA GGTGCAGGAG TACCAGGAGG CGCTGGAGGG CATCCTCATC 
AAGCAGAAGG ATGGCATACG ACTGTTTCCA GAGCTCTACA GCGTTCCTCC AGATAAGGTG 
GAAGAGGAAT ACATGAACCC CCACACAGTG GAGAGGATCC CGATGGGGAA ATGTCCCCTG 
AAATGGGGCC AGTCCCTTTA TGTTCTGGGG AGACTCTTAT CCGAGGGGTT TCTTGCTCCT 
GGTGAGATTG ACCCTCTTAA CCGCCGTTTC TCCACCGTGC CCAAGCCGGA CGTGGTCGTT 
CAGGTTTCCA TTTTGGCTGA AACAGATGAG ATTAAGGAGC TTTTGCTGAA GAACGGCTTC 
GAGGTGGAAA CCATCGCAGA CATCCACCCC TTTCATGTGC AGCCCTCCAG AGTCCTCAGC 
CACATCTATG CTAGGCTCGG ACGTAACCCA AGGCTGGGCC TGACGGGACG GCCCTACAGG 
AGAATCGGAG TTCTGGGAAC CTCAAAGTTC TACATCATCA GAAACACCAT CTTCACATTC 
ACTCCACAGT TCATCGACCA CCAGCAGTTC TACCTGGCTC TGGACAACAA AATGATCGTA 
GAGATGTTGA GGACAGAAAT CGCCTACCTG TCCTCCAGGT GGAGGATAAC CGGACGGCCG 
ACGGTCACCT TCCCCGTCTC TCAAACCATG CTGACTGAAG ACCGCACAGA TCTGGACCCG 
GCTGTCCTGG CGACGCTGAA GAAGCTACAG GATGGGTATT TTGGAGGAGC CAGGATCCAG 
ACGGGAAAGC TGTCAGAGTT CCTGACCACC TCCTGCTGCG CTCACCTGAG CTTCCTGGAT 
GAGGCAGACG ACCTTGCTCA GTACTTGGAT CACCTCCTGG CTCACTCTGC TTCTAAGAAG 
CAGAAGTCCA GCGCTGAAGG TGTGGGCCGA TTCAAAGCTG TTGCTACAAG AACCAGGGAG 
ATGGTCTCTT TGATGAACAA GGCTCAGGAT CTTAACATCC ACAACATTGG CATGTACCTT 
CCCAACAAGC TGTTTCGTAC TCGTCATCCA TCGCTCAATC TGAACCTTCC AGACAGTACT 
GTCCACGAAG AAGCTCAGGC ACCAGAGGTG TCCATCACCA CAGAAAGTAG CCTCCCCAGA 
GATGCCAGCG GCGCCATCGA CTACAGTGCT TTGGTCCAGC TGCTGAAAGA CAGTGGGAAC 
ATCCAGGACC AGGCAGATAT TCTCTACATC CTCTTCAAAG ACAAGGGGAT GGACTGGGAC 
ACACACCTTC ATGGGAGGGG CTCCACTGTC CGGTCTCTGC TCAGCAACCT TTACGAGAAG 
GCTGGTGACC TCAAACACTG GGGCCTGATC CGGATGATCT CTGGGATGCT GAAGAAGAAG 
GTGGAAGAGC TCGACTCGGC CTGCTCTGAC CTGCTGGCTC ACCAGAAGCA CCTGACAGTA 
GGTCTGCCTC CAGAACCCAG GGAGAAAACC ATCACGGCGC CCATCCCTCC AGACCAGCTG 
GCTGCCCTCA TCGATGAGGC CAGCGATGGC AACATCAGTG TTGCCATCCT CACACAGGAG 
ATCATGGTGT TCCTGGCCAT GAGCATCCGC ACGCAGCCCA GTCTGTTCAG CGAGATGTTC 
AGGCTCCGGA TCGGCCTCAT CATTCAAGTC ATGGCCACAG AACTGGCTCA GTCCCTCAAC 
TGCTCTGGTG ACGAGGCCAC AGAGAGTCTG ATGAGTCTGA GTCCGTCAGA GCTGAAGAAC 
CTGCTCCACC ACATCCTCAG TGGGAAAGAG TTTGGCGTGG AGCGCAGCGT CAGAGAGGGA 
GATGATGGCA TCAGTCCTGC CATCTCCATC CACCACCTGG GGAACGTTGG CGCCACCAAG 
AGTGAGAGAG CGGGCATCAG CAAGCTGAAG AGCGACATAA AGATGGGCGT TCAAAGTCCG 
TCGCTGGATA TGGAAAATGT GGAATCGGGG AGGTACCGGC TGCCGTCCAT AGAGTCTCTG 
GACATTTCGG ACAGCATTCC TACCATCAAG GACACCAGAC ATGGACAGTG GCTGCGCAGG 
AGACGGCTGG ACGGCGCGCT GAACCGAGTT CCTGTGGGCT TCTACCAGAA AGTGTGGAGG 
ATCCTGCAGA AGTGCCACGG TCTGTCCATC GAGGGCTTTG TTCTTCCATC ATCCACCACC 
AGAGAGATGA CCCCCGCAGA AATCAAGTTC TCAGTTCACG TGGAAACCGT TCTGAACCGC 
GTCCCCCAGC CCGAGTACCG GCAGCTGCTG GTGGAAGCCA TTCTGGTCCT CACCATGCTG 
GCCGACGTGG ACATCCCAAG CATCGGCTCC ATCATCCATG TGGAGAAGAT CGTCCATTTG 
GCCAACGATA TGTTCTACAA AGATCAGAAG GACCTGGGGG CCGAGGGGCA CATCCTAGAA 
AAAGATCCGT CCACGGGAGT GTGCCGACTC CTGTATGACA GCGCCCCCAG TGGTCGCTTT 
GGAAGCATGA CCTACCTGAC CAAGGCGGTG GCGGCTTATG TACAGGACTT CCTGCCTAGC 
GGGGCGTGTG CCGTGCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011485974.1
CDS	187..3747
Translation	MRSRSNSGVKLDNYARIVQQTILQHQDPVTGLLPGSLEQPDAWVRDNVYSIVSVWALSLAYRKNADRDEDKAKAYELEQSVVKLMRGVLQCIMRQLDKVEKFKYSRSTKDSLHAKYNPRTCATVVGDHQWGHLQVDATSLFLLFLAQMTASGLHIIYTQDEVDVVQNLMFYIEAAYKVADYGMWERGDKTNQGITEINASSIGMAKAALEALDELNLFGAKGGPGSVVHALADDIQHCQSILTSMLPKASISKEVDAGVLAILTYPAFAVEDMSIVTMTKEEIISKLQGRYGCCRFLRDGHKTPKEDPNRLYYESAELKLFENIECEWPLFWTYLILDGIFSNSHEQVQEYQEALEGILIKQKDGIRLFPELYSVPPDKVEEEYMNPHTVERIPMGKCPLKWGQSLYVLGRLLSEGFLAPGEIDPLNRRFSTVPKPDVVVQVSILAETDEIKELLLKNGFEVETIADIHPFHVQPSRVLSHIYARLGRNPRLGLTGRPYRRIGVLGTSKFYIIRNTIFTFTPQFIDHQQFYLALDNKMIVEMLRTEIAYLSSRWRITGRPTVTFPVSQTMLTEDRTDLDPAVLATLKKLQDGYFGGARIQTGKLSEFLTTSCCAHLSFLDEADDLAQYLDHLLAHSASKKQKSSAEGVGRFKAVATRTREMVSLMNKAQDLNIHNIGMYLPNKLFRTRHPSLNLNLPDSTVHEEAQAPEVSITTESSLPRDASGAIDYSALVQLLKDSGNIQDQADILYILFKDKGMDWDTHLHGRGSTVRSLLSNLYEKAGDLKHWGLIRMISGMLKKKVEELDSACSDLLAHQKHLTVGLPPEPREKTITAPIPPDQLAALIDEASDGNISVAILTQEIMVFLAMSIRTQPSLFSEMFRLRIGLIIQVMATELAQSLNCSGDEATESLMSLSPSELKNLLHHILSGKEFGVERSVREGDDGISPAISIHHLGNVGATKSERAGISKLKSDIKMGVQSPSLDMENVESGRYRLPSIESLDISDSIPTIKDTRHGQWLRRRRLDGALNRVPVGFYQKVWRILQKCHGLSIEGFVLPSSTTREMTPAEIKFSVHVETVLNRVPQPEYRQLLVEAILVLTMLADVDIPSIGSIIHVEKIVHLANDMFYKDQKDLGAEGHILEKDPSTGVCRLLYDSAPSGRFGSMTYLTKAVAAYVQDFLPSGACAVQ

Target ORF information:

RefSeq Version	XM_011487672.3
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes phosphorylase kinase regulatory subunit alpha 1 (phka1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011487672.3

ORF Insert Sequence:

ATGAGGAGCC GAAGCAACTC CGGCGTGAAG CTGGACAACT ATGCCCGGAT CGTCCAGCAG 
ACCATCCTGC AGCACCAGGA TCCAGTAACG GGTCTCCTTC CGGGCAGCTT GGAGCAGCCA 
GATGCATGGG TGAGGGACAA CGTCTACAGC ATCGTCTCAG TGTGGGCCCT CAGCCTGGCC 
TACAGGAAAA ATGCCGACCG GGACGAGGAC AAGGCCAAGG CCTACGAGCT GGAGCAGAGT 
GTGGTGAAGC TCATGAGAGG TGTCCTCCAG TGCATTATGA GACAGCTAGA CAAAGTGGAG 
AAGTTCAAAT ACAGCCGAAG TACCAAGGAC TCACTTCACG CCAAGTACAA CCCACGCACC 
TGTGCTACAG TGGTCGGGGA CCACCAGTGG GGTCACCTGC AGGTGGACGC AACCTCGCTC 
TTCCTGCTCT TCCTCGCTCA GATGACGGCA TCCGGTCTGC ACATCATCTA CACGCAGGAT 
GAGGTGGACG TCGTTCAGAA CCTCATGTTC TACATCGAGG CAGCTTACAA AGTGGCTGAT 
TATGGGATGT GGGAGAGGGG GGACAAAACC AACCAGGGCA TCACAGAGAT CAATGCCAGC 
TCCATCGGCA TGGCCAAGGC GGCTCTGGAG GCCCTGGACG AACTCAACCT GTTTGGAGCT 
AAGGGAGGAC CGGGATCTGT GGTCCACGCT CTGGCTGATG ACATCCAGCA CTGCCAGTCC 
ATCCTCACAT CCATGTTACC CAAAGCTTCC ATCTCTAAAG AGGTGGATGC CGGAGTTTTG 
GCTATTCTTA CCTACCCCGC CTTCGCCGTG GAGGACATGA GCATCGTCAC CATGACCAAG 
GAGGAGATCA TCTCCAAGCT GCAGGGTCGG TACGGCTGTT GCAGGTTTCT CAGGGACGGA 
CATAAAACAC CCAAAGAGGA TCCCAACCGC CTGTACTACG AGTCTGCAGA GCTGAAGCTG 
TTCGAGAACA TCGAGTGTGA GTGGCCTCTC TTCTGGACGT ACCTCATTCT GGACGGCATA 
TTCAGCAACA GTCATGAACA GGTGCAGGAG TACCAGGAGG CGCTGGAGGG CATCCTCATC 
AAGCAGAAGG ATGGCATACG ACTGTTTCCA GAGCTCTACA GCGTTCCTCC AGATAAGGTG 
GAAGAGGAAT ACATGAACCC CCACACAGTG GAGAGGATCC CGATGGGGAA ATGTCCCCTG 
AAATGGGGCC AGTCCCTTTA TGTTCTGGGG AGACTCTTAT CCGAGGGGTT TCTTGCTCCT 
GGTGAGATTG ACCCTCTTAA CCGCCGTTTC TCCACCGTGC CCAAGCCGGA CGTGGTCGTT 
CAGGTTTCCA TTTTGGCTGA AACAGATGAG ATTAAGGAGC TTTTGCTGAA GAACGGCTTC 
GAGGTGGAAA CCATCGCAGA CATCCACCCC TTTCATGTGC AGCCCTCCAG AGTCCTCAGC 
CACATCTATG CTAGGCTCGG ACGTAACCCA AGGCTGGGCC TGACGGGACG GCCCTACAGG 
AGAATCGGAG TTCTGGGAAC CTCAAAGTTC TACATCATCA GAAACACCAT CTTCACATTC 
ACTCCACAGT TCATCGACCA CCAGCAGTTC TACCTGGCTC TGGACAACAA AATGATCGTA 
GAGATGTTGA GGACAGAAAT CGCCTACCTG TCCTCCAGGT GGAGGATAAC CGGACGGCCG 
ACGGTCACCT TCCCCGTCTC TCAAACCATG CTGACTGAAG ACCGCACAGA TCTGGACCCG 
GCTGTCCTGG CGACGCTGAA GAAGCTACAG GATGGGTATT TTGGAGGAGC CAGGATCCAG 
ACGGGAAAGC TGTCAGAGTT CCTGACCACC TCCTGCTGCG CTCACCTGAG CTTCCTGGAT 
GAGGCAGACG ACCTTGCTCA GTACTTGGAT CACCTCCTGG CTCACTCTGC TTCTAAGAAG 
CAGAAGTCCA GCGCTGAAGG TGTGGGCCGA TTCAAAGCTG TTGCTACAAG AACCAGGGAG 
ATGGTCTCTT TGATGAACAA GGCTCAGGAT CTTAACATCC ACAACATTGG CATGTACCTT 
CCCAACAAGC TGTTTCGTAC TCGTCATCCA TCGCTCAATC TGAACCTTCC AGACAGTACT 
GTCCACGAAG AAGCTCAGGC ACCAGAGGTG TCCATCACCA CAGAAAGTAG CCTCCCCAGA 
GATGCCAGCG GCGCCATCGA CTACAGTGCT TTGGTCCAGC TGCTGAAAGA CAGTGGGAAC 
ATCCAGGACC AGGCAGATAT TCTCTACATC CTCTTCAAAG ACAAGGGGAT GGACTGGGAC 
ACACACCTTC ATGGGAGGGG CTCCACTGTC CGGTCTCTGC TCAGCAACCT TTACGAGAAG 
GCTGGTGACC TCAAACACTG GGGCCTGATC CGGATGATCT CTGGGATGCT GAAGAAGAAG 
GTGGAAGAGC TCGACTCGGC CTGCTCTGAC CTGCTGGCTC ACCAGAAGCA CCTGACAGTA 
GGTCTGCCTC CAGAACCCAG GGAGAAAACC ATCACGGCGC CCATCCCTCC AGACCAGCTG 
GCTGCCCTCA TCGATGAGGC CAGCGATGGC AACATCAGTG TTGCCATCCT CACACAGGAG 
ATCATGGTGT TCCTGGCCAT GAGCATCCGC ACGCAGCCCA GTCTGTTCAG CGAGATGTTC 
AGGCTCCGGA TCGGCCTCAT CATTCAAGTC ATGGCCACAG AACTGGCTCA GTCCCTCAAC 
TGCTCTGGTG ACGAGGCCAC AGAGAGTCTG ATGAGTCTGA GTCCGTCAGA GCTGAAGAAC 
CTGCTCCACC ACATCCTCAG TGGGAAAGAG TTTGGCGTGG AGCGCAGCGT CAGAGAGGGA 
GATGATGGCA TCAGTCCTGC CATCTCCATC CACCACCTGG GGAACGTTGG CGCCACCAAG 
AGTGAGAGAG CGGGCATCAG CAAGCTGAAG AGCGACATAA AGATGGGCGT TCAAAGTCCG 
TCGCTGGATA TGGAAAATGT GGAATCGGGG AGGTACCGGC TGCCGTCCAT AGAGTCTCTG 
GACATTTCGG ACAGCATTCC TACCATCAAG GACACCAGAC ATGGACAGTG GCTGCGCAGG 
AGACGGCTGG ACGGCGCGCT GAACCGAGTT CCTGTGGGCT TCTACCAGAA AGTGTGGAGG 
ATCCTGCAGA AGTGCCACGG TCTGTCCATC GAGGGCTTTG TTCTTCCATC ATCCACCACC 
AGAGAGATGA CCCCCGCAGA AATCAAGTTC TCAGTTCACG TGGAAACCGT TCTGAACCGC 
GTCCCCCAGC CCGAGTACCG GCAGCTGCTG GTGGAAGCCA TTCTGGTCCT CACCATGCTG 
GCCGACGTGG ACATCCCAAG CATCGGCTCC ATCATCCATG TGGAGAAGAT CGTCCATTTG 
GCCAACGATA TGTTCTACAA AGATCAGAAG GACCTGGGGG CCGAGGGGCA CATCCTAGAA 
AAAGATCCGT CCACGGGAGT GTGCCGACTC CTGTATGACA GCGCCCCCAG TGGTCGCTTT 
GGAAGCATGA CCTACCTGAC CAAGGCGGTG GCGGCTTATG TACAGGACTT CCTGCCTAGC 
GGGGCGTGTG CCGTGCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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03-6811-6572

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オンライン注文

phka1 cDNA ORF clone, Oryzias latipes(Japanese medaka)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム