TNF cDNA ORF clone, Oryctolagus cuniculus(rabbit)

Gene
Clones
gRNAs

Summary

Gene Symbol	TNF
Entrez Gene ID	100009088
Full Name	tumor necrosis factor
Synonyms	TNFA,TNFSF2,TNLG1F
Gene Type	protein-coding
Organism	Oryctolagus cuniculus(rabbit)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017344776.1	XP_017200265.1	tumor necrosis factor isoform X3
XM_008262537.2	XP_008260759.1	tumor necrosis factor isoform X1
XM_017344775.1	XP_017200264.1	tumor necrosis factor isoform X2
NM_001082263.1	NP_001075732.1	tumor necrosis factor precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Acute exposure to vibration is an apoptosis-inducing stimulus in the vocal fold epithelium.
Novaleski CK, Kimball EE, Mizuta M, Rousseau B
Tissue & cell48(5)407-16(2016 Oct)

Comparative genomic analysis of eutherian tumor necrosis factor ligand genes.
Premzl M
Immunogenetics68(2)125-32(2016 Feb)

Activity of matrix metalloproteinases 2 and 9 in cultured rabbit corneal epithelium cells stimulated by tumor necrosis factor alpha.
Wu ZQ, Zhang ZL, Nie SW, Yuan J, Yang YN
Genetics and molecular research : GMR14(2)6360-8(2015 Jun)

Role of the JNK pathway on the expression of inflammatory factors in alveolar macrophages under mechanical ventilation.
Tong J, Zhou XD, Kolosov VP, Perelman JM
International immunopharmacology17(3)821-7(2013 Nov)

The COX-2 selective blocker etodolac inhibits TNF?-induced apoptosis in isolated rabbit articular chondrocytes.
Kumagai K, Kubo M, Imai S, Toyoda F, Maeda T, Okumura N, Matsuura H, Matsusue Y
International journal of molecular sciences14(10)19705-15(2013 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

TUNEL staining was positively correlated with TNF-a protein expression. Our findings suggest that apoptosis can be induced in the vocal fold epithelium after 120min of modal intensity phonation. In contrast, shorter durations of vibration exposure do not result in apoptosis signaling.
Title: Acute exposure to vibration is an apoptosis-inducing stimulus in the vocal fold epithelium.

Inflammatory factors such as TNF-alpha can stimulate MMP-2/9 activity in corneal epithelium cells. This may be a potential manipulating mechanism of MMP expression in the pathogenesis of corneal diseases.
Title: Activity of matrix metalloproteinases 2 and 9 in cultured rabbit corneal epithelium cells stimulated by tumor necrosis factor alpha.

The JNK pathway plays an important role in mechanical ventilation-stimulated TNF-alpha expression in alveolar macrophages, but the injury-stimulated IL-8 expression may be regulated by other signaling pathways.
Title: Role of the JNK pathway on the expression of inflammatory factors in alveolar macrophages under mechanical ventilation.

The TNFalpha-evoked Cl- current.
Title: The COX-2 selective blocker etodolac inhibits TNF?-induced apoptosis in isolated rabbit articular chondrocytes.

IL-1beta and TNF-alpha expression increases significantly during acute lung injury. Ambroxol combined with low-dose heparin inhibits teh release of IL-1beta and TNF-alpha.
Title: [Effects of ambroxol combined with low-dose heparin on TNF-alpha and IL-1beta in rabbits with acute lung injury].

The following TNF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TNF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OOb00337	NM_001082263.1 Latest version!	Oryctolagus cuniculus tumor necrosis factor (TNF), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OOb11657	XM_008262537.2 Latest version!	Oryctolagus cuniculus tumor necrosis factor (TNF), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OOb35117	XM_017344775.1 Latest version!	Oryctolagus cuniculus tumor necrosis factor (TNF), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00
OOb35118	XM_017344776.1 Latest version!	Oryctolagus cuniculus tumor necrosis factor (TNF), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TNF ( NM_001082263.1 ) cDNA ORF clone, Oryctolagus cuniculus(rabbit) -> NP_001075732.1 Oryctolagus cuniculus tumor necrosis factor (TNF), mRNA.

Price & Availability↑

CloneID	OOb00337
Clone ID Related Accession (Same CDS sequence)	NM_001082263.1
Accession Version	NM_001082263.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 705bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-22
Organism	Oryctolagus cuniculus(rabbit)
Product	tumor necrosis factor precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from M12845.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M12845.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00013641, SAMN00013655 [ECO:0000348] ##Evidence-Data-END##

ATGAGCACTG AGAGTATGAT CCGGGACGTC GAGCTGGCGG AGGGGCCGCT CCCCAAGAAG 
GCAGGGGGGC CCCAGGGCTC CAAGCGCTGC CTCTGCCTCA GCCTCTTCTC TTTCCTGCTC 
GTGGCTGGAG CCACCACGCT CTTCTGCCTG CTGCACTTCA GGGTGATCGG CCCTCAGGAG 
GAAGAGTCCC CAAACAACCT CCATCTAGTC AACCCTGTGG CCCAGATGGT CACCCTCAGA 
TCAGCTTCTC GGGCCCTGAG TGACAAGCCT CTAGCCCACG TAGTAGCAAA CCCGCAAGTG 
GAGGGCCAGC TCCAGTGGCT GAGCCAGCGT GCGAACGCCC TGCTGGCCAA CGGCATGAAG 
CTCACGGACA ACCAGCTGGT GGTGCCGGCC GACGGGCTGT ACCTCATCTA CTCCCAGGTT 
CTCTTCAGCG GTCAAGGCTG CCGCTCCTAC GTGCTCCTCA CTCACACTGT CAGCCGCTTC 
GCCGTCTCCT ACCCGAACAA GGTCAACCTC CTCTCTGCCA TCAAGAGCCC CTGCCACCGG 
GAGACCCCCG AGGAGGCTGA GCCCATGGCC TGGTACGAGC CCATCTACCT GGGCGGCGTC 
TTCCAGTTGG AGAAGGGTGA CCGGCTCAGC ACCGAGGTCA ACCAGCCTGA GTACCTGGAC 
CTTGCCGAGT CCGGGCAGGT CTACTTTGGG ATCATTGCCC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001075732.1
CDS	174..878
Translation	MSTESMIRDVELAEGPLPKKAGGPQGSKRCLCLSLFSFLLVAGATTLFCLLHFRVIGPQEEESPNNLHLVNPVAQMVTLRSASRALSDKPLAHVVANPQVEGQLQWLSQRANALLANGMKLTDNQLVVPADGLYLIYSQVLFSGQGCRSYVLLTHTVSRFAVSYPNKVNLLSAIKSPCHRETPEEAEPMAWYEPIYLGGVFQLEKGDRLSTEVNQPEYLDLAESGQVYFGIIAL

Target ORF information:

RefSeq Version	NM_001082263.1
Organism	Oryctolagus cuniculus(rabbit)
Definition	Oryctolagus cuniculus tumor necrosis factor (TNF), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001082263.1

ORF Insert Sequence:

ATGAGCACTG AGAGTATGAT CCGGGACGTC GAGCTGGCGG AGGGGCCGCT CCCCAAGAAG 
GCAGGGGGGC CCCAGGGCTC CAAGCGCTGC CTCTGCCTCA GCCTCTTCTC TTTCCTGCTC 
GTGGCTGGAG CCACCACGCT CTTCTGCCTG CTGCACTTCA GGGTGATCGG CCCTCAGGAG 
GAAGAGTCCC CAAACAACCT CCATCTAGTC AACCCTGTGG CCCAGATGGT CACCCTCAGA 
TCAGCTTCTC GGGCCCTGAG TGACAAGCCT CTAGCCCACG TAGTAGCAAA CCCGCAAGTG 
GAGGGCCAGC TCCAGTGGCT GAGCCAGCGT GCGAACGCCC TGCTGGCCAA CGGCATGAAG 
CTCACGGACA ACCAGCTGGT GGTGCCGGCC GACGGGCTGT ACCTCATCTA CTCCCAGGTT 
CTCTTCAGCG GTCAAGGCTG CCGCTCCTAC GTGCTCCTCA CTCACACTGT CAGCCGCTTC 
GCCGTCTCCT ACCCGAACAA GGTCAACCTC CTCTCTGCCA TCAAGAGCCC CTGCCACCGG 
GAGACCCCCG AGGAGGCTGA GCCCATGGCC TGGTACGAGC CCATCTACCT GGGCGGCGTC 
TTCCAGTTGG AGAAGGGTGA CCGGCTCAGC ACCGAGGTCA ACCAGCCTGA GTACCTGGAC 
CTTGCCGAGT CCGGGCAGGT CTACTTTGGG ATCATTGCCC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNF ( XM_008262537.2 ) cDNA ORF clone, Oryctolagus cuniculus(rabbit) -> XP_008260759.1 Oryctolagus cuniculus tumor necrosis factor (TNF), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OOb11657
Clone ID Related Accession (Same CDS sequence)	XM_008262537.2
Accession Version	XM_008262537.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 708bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-22
Organism	Oryctolagus cuniculus(rabbit)
Product	tumor necrosis factor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_013680.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 23, 2016 this sequence version replaced XM_008262537.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryctolagus cuniculus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCACTG AGAGTATGAT CCGGGACGTC GAGCTGGCGG AGGGGCCGCT CCCCAAGAAG 
GCAGGGGGGC CCCAGGGCTC CAAGCGTTGC CTCTGCCTCA GCCTCTTCTC TTTCCTGCTC 
GTGGCTGGAG CCACCACGCT CTTCTGCCTG CTGCACTTCA GGGTGATCGG CCCTCAGGAG 
GAAGAGCAGT CCCCAAACAA CCTCCATCTA GTCAACCCTG TGGCCCAGAT GGTCACCCTC 
AGATCAGCTT CTCGGGCCCT GAGTGACAAG CCTCTAGCCC ACGTAGTAGC AAACCCGCAA 
GTGGAGGGCC AGCTCCAGTG GCTGAGCCAG CGTGCGAACG CCCTGCTGGC CAACGGCATG 
AAGCTCACGG ACAACCAGCT GGTGGTGCCG GCCGACGGGC TGTACCTCAT CTACTCCCAG 
GTTCTCTTCA GCGGTCAAGG CTGCCGCTCC TACGTGCTCC TCACTCACAC TGTCAGCCGC 
TTCGCCGTCT CCTACCCGAA CAAGGTCAAC CTCCTCTCTG CCATCAAGAG CCCCTGCCAC 
CGGGAGACCC CCGAGGAGGC TGAGCCCATG GCCTGGTACG AGCCCATCTA CCTGGGCGGC 
GTCTTCCAGT TGGAGAAGGG TGACCGGCTC AGCACCGAGG TCAACCAGCC TGAGTACCTG 
GACCTTGCCG AGTCCGGGCA GGTCTACTTT GGGATCATTG CCCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008260759.1
CDS	189..896
Translation	MSTESMIRDVELAEGPLPKKAGGPQGSKRCLCLSLFSFLLVAGATTLFCLLHFRVIGPQEEEQSPNNLHLVNPVAQMVTLRSASRALSDKPLAHVVANPQVEGQLQWLSQRANALLANGMKLTDNQLVVPADGLYLIYSQVLFSGQGCRSYVLLTHTVSRFAVSYPNKVNLLSAIKSPCHRETPEEAEPMAWYEPIYLGGVFQLEKGDRLSTEVNQPEYLDLAESGQVYFGIIAL

Target ORF information:

RefSeq Version	XM_008262537.2
Organism	Oryctolagus cuniculus(rabbit)
Definition	Oryctolagus cuniculus tumor necrosis factor (TNF), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008262537.2

ORF Insert Sequence:

ATGAGCACTG AGAGTATGAT CCGGGACGTC GAGCTGGCGG AGGGGCCGCT CCCCAAGAAG 
GCAGGGGGGC CCCAGGGCTC CAAGCGTTGC CTCTGCCTCA GCCTCTTCTC TTTCCTGCTC 
GTGGCTGGAG CCACCACGCT CTTCTGCCTG CTGCACTTCA GGGTGATCGG CCCTCAGGAG 
GAAGAGCAGT CCCCAAACAA CCTCCATCTA GTCAACCCTG TGGCCCAGAT GGTCACCCTC 
AGATCAGCTT CTCGGGCCCT GAGTGACAAG CCTCTAGCCC ACGTAGTAGC AAACCCGCAA 
GTGGAGGGCC AGCTCCAGTG GCTGAGCCAG CGTGCGAACG CCCTGCTGGC CAACGGCATG 
AAGCTCACGG ACAACCAGCT GGTGGTGCCG GCCGACGGGC TGTACCTCAT CTACTCCCAG 
GTTCTCTTCA GCGGTCAAGG CTGCCGCTCC TACGTGCTCC TCACTCACAC TGTCAGCCGC 
TTCGCCGTCT CCTACCCGAA CAAGGTCAAC CTCCTCTCTG CCATCAAGAG CCCCTGCCAC 
CGGGAGACCC CCGAGGAGGC TGAGCCCATG GCCTGGTACG AGCCCATCTA CCTGGGCGGC 
GTCTTCCAGT TGGAGAAGGG TGACCGGCTC AGCACCGAGG TCAACCAGCC TGAGTACCTG 
GACCTTGCCG AGTCCGGGCA GGTCTACTTT GGGATCATTG CCCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNF ( XM_017344775.1 ) cDNA ORF clone, Oryctolagus cuniculus(rabbit) -> XP_017200264.1 Oryctolagus cuniculus tumor necrosis factor (TNF), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OOb35117
Clone ID Related Accession (Same CDS sequence)	XM_017344775.1
Accession Version	XM_017344775.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 660bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-22
Organism	Oryctolagus cuniculus(rabbit)
Product	tumor necrosis factor isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_013680.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryctolagus cuniculus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCACTG AGAGTATGAT CCGGGACGTC GAGCTGGCGG AGGGGCCGCT CCCCAAGAAG 
GCAGGGGGGC CCCAGGGCTC CAAGCGTTGC CTCTGCCTCA GCCTCTTCTC TTTCCTGCTC 
GTGGCTGGAG CCACCACGCT CTTCTGCCTG CTGCACTTCA GGGTGATCGG CCCTCAGGAG 
GAAGAGCAGT CCCCAAACAA CCTCCATCTA GTCAACCCTG TGGCCCAGAT GGTCACCCTC 
ACAAACCCGC AAGTGGAGGG CCAGCTCCAG TGGCTGAGCC AGCGTGCGAA CGCCCTGCTG 
GCCAACGGCA TGAAGCTCAC GGACAACCAG CTGGTGGTGC CGGCCGACGG GCTGTACCTC 
ATCTACTCCC AGGTTCTCTT CAGCGGTCAA GGCTGCCGCT CCTACGTGCT CCTCACTCAC 
ACTGTCAGCC GCTTCGCCGT CTCCTACCCG AACAAGGTCA ACCTCCTCTC TGCCATCAAG 
AGCCCCTGCC ACCGGGAGAC CCCCGAGGAG GCTGAGCCCA TGGCCTGGTA CGAGCCCATC 
TACCTGGGCG GCGTCTTCCA GTTGGAGAAG GGTGACCGGC TCAGCACCGA GGTCAACCAG 
CCTGAGTACC TGGACCTTGC CGAGTCCGGG CAGGTCTACT TTGGGATCAT TGCCCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017200264.1
CDS	190..849
Translation	MSTESMIRDVELAEGPLPKKAGGPQGSKRCLCLSLFSFLLVAGATTLFCLLHFRVIGPQEEEQSPNNLHLVNPVAQMVTLTNPQVEGQLQWLSQRANALLANGMKLTDNQLVVPADGLYLIYSQVLFSGQGCRSYVLLTHTVSRFAVSYPNKVNLLSAIKSPCHRETPEEAEPMAWYEPIYLGGVFQLEKGDRLSTEVNQPEYLDLAESGQVYFGIIAL

Target ORF information:

RefSeq Version	XM_017344775.1
Organism	Oryctolagus cuniculus(rabbit)
Definition	Oryctolagus cuniculus tumor necrosis factor (TNF), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017344775.1

ORF Insert Sequence:

ATGAGCACTG AGAGTATGAT CCGGGACGTC GAGCTGGCGG AGGGGCCGCT CCCCAAGAAG 
GCAGGGGGGC CCCAGGGCTC CAAGCGTTGC CTCTGCCTCA GCCTCTTCTC TTTCCTGCTC 
GTGGCTGGAG CCACCACGCT CTTCTGCCTG CTGCACTTCA GGGTGATCGG CCCTCAGGAG 
GAAGAGCAGT CCCCAAACAA CCTCCATCTA GTCAACCCTG TGGCCCAGAT GGTCACCCTC 
ACAAACCCGC AAGTGGAGGG CCAGCTCCAG TGGCTGAGCC AGCGTGCGAA CGCCCTGCTG 
GCCAACGGCA TGAAGCTCAC GGACAACCAG CTGGTGGTGC CGGCCGACGG GCTGTACCTC 
ATCTACTCCC AGGTTCTCTT CAGCGGTCAA GGCTGCCGCT CCTACGTGCT CCTCACTCAC 
ACTGTCAGCC GCTTCGCCGT CTCCTACCCG AACAAGGTCA ACCTCCTCTC TGCCATCAAG 
AGCCCCTGCC ACCGGGAGAC CCCCGAGGAG GCTGAGCCCA TGGCCTGGTA CGAGCCCATC 
TACCTGGGCG GCGTCTTCCA GTTGGAGAAG GGTGACCGGC TCAGCACCGA GGTCAACCAG 
CCTGAGTACC TGGACCTTGC CGAGTCCGGG CAGGTCTACT TTGGGATCAT TGCCCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNF ( XM_017344776.1 ) cDNA ORF clone, Oryctolagus cuniculus(rabbit) -> XP_017200265.1 Oryctolagus cuniculus tumor necrosis factor (TNF), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OOb35118
Clone ID Related Accession (Same CDS sequence)	XM_017344776.1
Accession Version	XM_017344776.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 657bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-22
Organism	Oryctolagus cuniculus(rabbit)
Product	tumor necrosis factor isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_013680.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryctolagus cuniculus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCACTG AGAGTATGAT CCGGGACGTC GAGCTGGCGG AGGGGCCGCT CCCCAAGAAG 
GCAGGGGGGC CCCAGGGCTC CAAGCGTTGC CTCTGCCTCA GCCTCTTCTC TTTCCTGCTC 
GTGGCTGGAG CCACCACGCT CTTCTGCCTG CTGCACTTCA GGGTGATCGG CCCTCAGGAG 
GAAGAGTCCC CAAACAACCT CCATCTAGTC AACCCTGTGG CCCAGATGGT CACCCTCACA 
AACCCGCAAG TGGAGGGCCA GCTCCAGTGG CTGAGCCAGC GTGCGAACGC CCTGCTGGCC 
AACGGCATGA AGCTCACGGA CAACCAGCTG GTGGTGCCGG CCGACGGGCT GTACCTCATC 
TACTCCCAGG TTCTCTTCAG CGGTCAAGGC TGCCGCTCCT ACGTGCTCCT CACTCACACT 
GTCAGCCGCT TCGCCGTCTC CTACCCGAAC AAGGTCAACC TCCTCTCTGC CATCAAGAGC 
CCCTGCCACC GGGAGACCCC CGAGGAGGCT GAGCCCATGG CCTGGTACGA GCCCATCTAC 
CTGGGCGGCG TCTTCCAGTT GGAGAAGGGT GACCGGCTCA GCACCGAGGT CAACCAGCCT 
GAGTACCTGG ACCTTGCCGA GTCCGGGCAG GTCTACTTTG GGATCATTGC CCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017200265.1
CDS	191..847
Translation	MSTESMIRDVELAEGPLPKKAGGPQGSKRCLCLSLFSFLLVAGATTLFCLLHFRVIGPQEEESPNNLHLVNPVAQMVTLTNPQVEGQLQWLSQRANALLANGMKLTDNQLVVPADGLYLIYSQVLFSGQGCRSYVLLTHTVSRFAVSYPNKVNLLSAIKSPCHRETPEEAEPMAWYEPIYLGGVFQLEKGDRLSTEVNQPEYLDLAESGQVYFGIIAL

Target ORF information:

RefSeq Version	XM_017344776.1
Organism	Oryctolagus cuniculus(rabbit)
Definition	Oryctolagus cuniculus tumor necrosis factor (TNF), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017344776.1

ORF Insert Sequence:

ATGAGCACTG AGAGTATGAT CCGGGACGTC GAGCTGGCGG AGGGGCCGCT CCCCAAGAAG 
GCAGGGGGGC CCCAGGGCTC CAAGCGTTGC CTCTGCCTCA GCCTCTTCTC TTTCCTGCTC 
GTGGCTGGAG CCACCACGCT CTTCTGCCTG CTGCACTTCA GGGTGATCGG CCCTCAGGAG 
GAAGAGTCCC CAAACAACCT CCATCTAGTC AACCCTGTGG CCCAGATGGT CACCCTCACA 
AACCCGCAAG TGGAGGGCCA GCTCCAGTGG CTGAGCCAGC GTGCGAACGC CCTGCTGGCC 
AACGGCATGA AGCTCACGGA CAACCAGCTG GTGGTGCCGG CCGACGGGCT GTACCTCATC 
TACTCCCAGG TTCTCTTCAG CGGTCAAGGC TGCCGCTCCT ACGTGCTCCT CACTCACACT 
GTCAGCCGCT TCGCCGTCTC CTACCCGAAC AAGGTCAACC TCCTCTCTGC CATCAAGAGC 
CCCTGCCACC GGGAGACCCC CGAGGAGGCT GAGCCCATGG CCTGGTACGA GCCCATCTAC 
CTGGGCGGCG TCTTCCAGTT GGAGAAGGGT GACCGGCTCA GCACCGAGGT CAACCAGCCT 
GAGTACCTGG ACCTTGCCGA GTCCGGGCAG GTCTACTTTG GGATCATTGC CCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Acute exposure to vibration is an apoptosis-inducing stimulus in the vocal fold epithelium.
Tissue & cell48(5)407-16(2016 Oct)
Novaleski CK,Kimball EE,Mizuta M,Rousseau B

Comparative genomic analysis of eutherian tumor necrosis factor ligand genes.
Immunogenetics68(2)125-32(2016 Feb)
Premzl M

Activity of matrix metalloproteinases 2 and 9 in cultured rabbit corneal epithelium cells stimulated by tumor necrosis factor alpha.
Genetics and molecular research : GMR14(2)6360-8(2015 Jun)
Wu ZQ,Zhang ZL,Nie SW,Yuan J,Yang YN

Role of the JNK pathway on the expression of inflammatory factors in alveolar macrophages under mechanical ventilation.
International immunopharmacology17(3)821-7(2013 Nov)
Tong J,Zhou XD,Kolosov VP,Perelman JM

The COX-2 selective blocker etodolac inhibits TNF?-induced apoptosis in isolated rabbit articular chondrocytes.
International journal of molecular sciences14(10)19705-15(2013 Sep)
Kumagai K,Kubo M,Imai S,Toyoda F,Maeda T,Okumura N,Matsuura H,Matsusue Y