PYGM cDNA ORF clone, Orycteropus afer afer

Gene
Clones
gRNAs

Summary

Gene Symbol	PYGM
Entrez Gene ID	103200328
Full Name	glycogen phosphorylase, muscle associated
Gene Type	protein-coding
Organism	Orycteropus afer afer

mRNA and Protein(s)

mRNA	Protein	Name
XM_007944805.1	XP_007942996.1	glycogen phosphorylase, muscle form

Pathways from BioSystems
Gene Ontology

The following PYGM gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PYGM cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OOr113655	XM_007944805.1 Latest version!	Orycteropus afer afer phosphorylase, glycogen, muscle (PYGM), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PYGM ( XM_007944805.1 ) cDNA ORF clone, Orycteropus afer afer -> XP_007942996.1 Orycteropus afer afer phosphorylase, glycogen, muscle (PYGM), mRNA.

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CloneID	OOr113655
Clone ID Related Accession (Same CDS sequence)	XM_007944805.1
Accession Version	XM_007944805.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2529bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-05-13
Organism	Orycteropus afer afer
Product	glycogen phosphorylase, muscle form
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006921717.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Orycteropus afer afer Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCGGC CCATGACAGA CCAAGAGAAG AGGAAGCAAA TCAGTGTAAG AGGCCTGGCC 
GGCGTGGAGA ATGTGGCCGA ACTGAAGAAG AATTTCAACA GGCACCTGCA CTTCACACTG 
GTCAAGGACC GCAATGTGGC CACGCCCCGT GACTACTACT TCGCGCTGGC TCACACTGTA 
CGTGACCACC TGGTGGGCCG CTGGATCCGC ACGCAGCAAC ACTACTACGA GAAGGACCCC 
AAGAGGATCT ACTACCTGTC TCTGGAGTTC TACATTGGAC GGACGCTACA GAACACCATG 
GTGAACCTGG CCCTGGAGAA CGCCTGCGAT GAGGCCACCT ACCAGCTGGG CCTGGACATG 
GAGGAGCTAG AGGAAATCGA GGAAGATGCA GGCTTGGGGA ATGGGGGCCT GGGCCGGCTG 
GCAGCCTGCT TCCTGGACTC CATGGCGACA CTGGGCCTGG CTGCCTATGG CTACGGGATC 
CGCTACGAGT TTGGGATTTT TAACCAGAAG ATCACCGGAG GCTGGCAGAT GGAGGAAGCT 
GATGACTGGC TTCGCTACGG CAACCCCTGG GAAAAGGCCC GGCCCGAGTT CATGCTGCCT 
GTCCATTTCT ATGGCCGAGT GGAGCACACC AACCAGGGGG CCAAGTGGGT GGACACACAG 
GTGGTCCTGG CCATGCCATA TGACACGCCG GTGCCTGGCT ATCGTAACAA CGTTGTCAAC 
ACCATGCGCC TGTGGTCAGC CAAAGCCCCC AATGACTTCA ACCTCAAGGA CTTCAACGTC 
GGTGGCTACA TCCAGGCTGT GCTGGACCGT AACTTGGCTG AGAACATCTC CCGTGTCCTG 
TACCCCAACG ACAACTTCTT CGAGGGCAAG GAGCTTCGGC TGAAGCAGGA GTACTTCGTG 
GTGGCCGCTA CGCTCCAGGA CATCATCCGC CGCTTCAAGT CCTCCAAGTT CGGCTGCCGT 
GACCCTGTGC GCACGAGCTT TGATGCCTTC CCTGAAAAGG TAGCCATCCA GCTCAATGAC 
ACCCACCCAT CCTTGGCCAT CCCTGAGCTA ATGAGGATCC TGGTGGACCT GGAGCGGCTA 
GAATGGGAAA AGGCATGGGA CGTGACAGTG AAGACGTGCG CCTACACCAA CCACACGGTG 
CTGCCCGAGG CCCTGGAGCG CTGGCCCGTG CACCTCATGG AGACGCTGTT GCCTCGCCAC 
CTGCAGATCA TTTACGAGAT CAACCAGCGC TTCCTGAACC GGGTGGCAGC TGCATTCCCA 
GGGGATGTAG ACCGGCTGCG GCGCATGTCG CTGGTGGAGG AGGGCGCGGT GAAGCGCATC 
AACATGGCGC ACCTGTGCAT CGCCGGCTCG CACGCGGTCA ACGGCGTGGC CCGCATCCAC 
TCTGAGATCC TCAAGAAGAC TATCTTCAAG GACTTCTACG AGCTGGACCC TCATAAGTTC 
CAGAATAAGA CCAATGGCAT CACCCCTCGG CGCTGGCTGG TTCTCTGTAA CCCTGGGCTG 
GCAGAGGTCA TCGCCGAGCG CATCGGGGAA GACTACATCT CGGACCTGGA CCAACTGCGC 
AAACTGCTCT CCTACGTGGA TGACGAGGCT TTTATCCGGG ATGTGGCCAA AATCAAGCAG 
GAAAACAAGT TGAAATTCTC TGCATACCTG GAGAAGGAAT ATAAAGTACA CATCAACCCC 
AACTCGCTCT TCGATATCCA GGTGAAGCGG ATTCACGAAT ACAAACGCCA GCTCCTCAAC 
TGTCTGCACG TCATCACCCT GTACAACCGC ATCAAGAAGG AACCCAATAA GTTTGTGGTG 
CCTCGAACTG TGATGATTGG AGGGAAGGCT GCACCTGGGT ACCACATGGC CAAGATGATC 
ATCAAACTCA TCACATCCAT TGGGGATGTG GTCAACCATG ACCCAGTGGT GGGAGACCGC 
CTCCGCGTCA TCTTCCTGGA GAACTACCGA GTGTCTCTGG CGGAGAAAGT GATCCCAGCT 
GCCGATCTTT CTGAACAAAT CTCCACTGCG GGCACTGAGG CCTCAGGCAC TGGCAACATG 
AAGTTCATGC TCAACGGGGC TCTGACCATC GGCACCATGG ATGGCGCCAA CGTGGAGATG 
GCAGAAGAGG CGGGCGAGGA GAACTTCTTC ATCTTTGGCA TGCGCGTGGA GGATGTGGAC 
AGGCTCGACC AGAGAGGGTA CAATGCCCAG GAGTACTACG ACCGCATTCC CGAACTGCGG 
CAGGTCATTG ATCAGCTCAG CAGTGGCTTC TTCTCCCCGA AACAGCCCGA CCTGTTCAAG 
GATATTGTCA ACATGCTCAT GCACCACGAC CGGTTTAAAG TCTTTGCCGA TTATGAAGAC 
TACATTAAAT GCCAGGAGAA AGTCAGCGCC CTGTACAAGA ACCCGAGAGA GTGGACACGC 
ATGGTGATTC GGAACATCGC CACCTCGGGC AAGTTCTCCA GCGACCGCAC CATTGCCCAG 
TACGCCCGGG AGATCTGGAA AGTCGAGCCT TCCCGCCAAC GCCTGCCAGC CCCTGATGAG 
ACCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007942996.1
CDS	396..2924
Translation	MSRPMTDQEKRKQISVRGLAGVENVAELKKNFNRHLHFTLVKDRNVATPRDYYFALAHTVRDHLVGRWIRTQQHYYEKDPKRIYYLSLEFYIGRTLQNTMVNLALENACDEATYQLGLDMEELEEIEEDAGLGNGGLGRLAACFLDSMATLGLAAYGYGIRYEFGIFNQKITGGWQMEEADDWLRYGNPWEKARPEFMLPVHFYGRVEHTNQGAKWVDTQVVLAMPYDTPVPGYRNNVVNTMRLWSAKAPNDFNLKDFNVGGYIQAVLDRNLAENISRVLYPNDNFFEGKELRLKQEYFVVAATLQDIIRRFKSSKFGCRDPVRTSFDAFPEKVAIQLNDTHPSLAIPELMRILVDLERLEWEKAWDVTVKTCAYTNHTVLPEALERWPVHLMETLLPRHLQIIYEINQRFLNRVAAAFPGDVDRLRRMSLVEEGAVKRINMAHLCIAGSHAVNGVARIHSEILKKTIFKDFYELDPHKFQNKTNGITPRRWLVLCNPGLAEVIAERIGEDYISDLDQLRKLLSYVDDEAFIRDVAKIKQENKLKFSAYLEKEYKVHINPNSLFDIQVKRIHEYKRQLLNCLHVITLYNRIKKEPNKFVVPRTVMIGGKAAPGYHMAKMIIKLITSIGDVVNHDPVVGDRLRVIFLENYRVSLAEKVIPAADLSEQISTAGTEASGTGNMKFMLNGALTIGTMDGANVEMAEEAGEENFFIFGMRVEDVDRLDQRGYNAQEYYDRIPELRQVIDQLSSGFFSPKQPDLFKDIVNMLMHHDRFKVFADYEDYIKCQEKVSALYKNPREWTRMVIRNIATSGKFSSDRTIAQYAREIWKVEPSRQRLPAPDETI

Target ORF information:

RefSeq Version	XM_007944805.1
Organism	Orycteropus afer afer
Definition	Orycteropus afer afer phosphorylase, glycogen, muscle (PYGM), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007944805.1

ORF Insert Sequence:

ATGTCCCGGC CCATGACAGA CCAAGAGAAG AGGAAGCAAA TCAGTGTAAG AGGCCTGGCC 
GGCGTGGAGA ATGTGGCCGA ACTGAAGAAG AATTTCAACA GGCACCTGCA CTTCACACTG 
GTCAAGGACC GCAATGTGGC CACGCCCCGT GACTACTACT TCGCGCTGGC TCACACTGTA 
CGTGACCACC TGGTGGGCCG CTGGATCCGC ACGCAGCAAC ACTACTACGA GAAGGACCCC 
AAGAGGATCT ACTACCTGTC TCTGGAGTTC TACATTGGAC GGACGCTACA GAACACCATG 
GTGAACCTGG CCCTGGAGAA CGCCTGCGAT GAGGCCACCT ACCAGCTGGG CCTGGACATG 
GAGGAGCTAG AGGAAATCGA GGAAGATGCA GGCTTGGGGA ATGGGGGCCT GGGCCGGCTG 
GCAGCCTGCT TCCTGGACTC CATGGCGACA CTGGGCCTGG CTGCCTATGG CTACGGGATC 
CGCTACGAGT TTGGGATTTT TAACCAGAAG ATCACCGGAG GCTGGCAGAT GGAGGAAGCT 
GATGACTGGC TTCGCTACGG CAACCCCTGG GAAAAGGCCC GGCCCGAGTT CATGCTGCCT 
GTCCATTTCT ATGGCCGAGT GGAGCACACC AACCAGGGGG CCAAGTGGGT GGACACACAG 
GTGGTCCTGG CCATGCCATA TGACACGCCG GTGCCTGGCT ATCGTAACAA CGTTGTCAAC 
ACCATGCGCC TGTGGTCAGC CAAAGCCCCC AATGACTTCA ACCTCAAGGA CTTCAACGTC 
GGTGGCTACA TCCAGGCTGT GCTGGACCGT AACTTGGCTG AGAACATCTC CCGTGTCCTG 
TACCCCAACG ACAACTTCTT CGAGGGCAAG GAGCTTCGGC TGAAGCAGGA GTACTTCGTG 
GTGGCCGCTA CGCTCCAGGA CATCATCCGC CGCTTCAAGT CCTCCAAGTT CGGCTGCCGT 
GACCCTGTGC GCACGAGCTT TGATGCCTTC CCTGAAAAGG TAGCCATCCA GCTCAATGAC 
ACCCACCCAT CCTTGGCCAT CCCTGAGCTA ATGAGGATCC TGGTGGACCT GGAGCGGCTA 
GAATGGGAAA AGGCATGGGA CGTGACAGTG AAGACGTGCG CCTACACCAA CCACACGGTG 
CTGCCCGAGG CCCTGGAGCG CTGGCCCGTG CACCTCATGG AGACGCTGTT GCCTCGCCAC 
CTGCAGATCA TTTACGAGAT CAACCAGCGC TTCCTGAACC GGGTGGCAGC TGCATTCCCA 
GGGGATGTAG ACCGGCTGCG GCGCATGTCG CTGGTGGAGG AGGGCGCGGT GAAGCGCATC 
AACATGGCGC ACCTGTGCAT CGCCGGCTCG CACGCGGTCA ACGGCGTGGC CCGCATCCAC 
TCTGAGATCC TCAAGAAGAC TATCTTCAAG GACTTCTACG AGCTGGACCC TCATAAGTTC 
CAGAATAAGA CCAATGGCAT CACCCCTCGG CGCTGGCTGG TTCTCTGTAA CCCTGGGCTG 
GCAGAGGTCA TCGCCGAGCG CATCGGGGAA GACTACATCT CGGACCTGGA CCAACTGCGC 
AAACTGCTCT CCTACGTGGA TGACGAGGCT TTTATCCGGG ATGTGGCCAA AATCAAGCAG 
GAAAACAAGT TGAAATTCTC TGCATACCTG GAGAAGGAAT ATAAAGTACA CATCAACCCC 
AACTCGCTCT TCGATATCCA GGTGAAGCGG ATTCACGAAT ACAAACGCCA GCTCCTCAAC 
TGTCTGCACG TCATCACCCT GTACAACCGC ATCAAGAAGG AACCCAATAA GTTTGTGGTG 
CCTCGAACTG TGATGATTGG AGGGAAGGCT GCACCTGGGT ACCACATGGC CAAGATGATC 
ATCAAACTCA TCACATCCAT TGGGGATGTG GTCAACCATG ACCCAGTGGT GGGAGACCGC 
CTCCGCGTCA TCTTCCTGGA GAACTACCGA GTGTCTCTGG CGGAGAAAGT GATCCCAGCT 
GCCGATCTTT CTGAACAAAT CTCCACTGCG GGCACTGAGG CCTCAGGCAC TGGCAACATG 
AAGTTCATGC TCAACGGGGC TCTGACCATC GGCACCATGG ATGGCGCCAA CGTGGAGATG 
GCAGAAGAGG CGGGCGAGGA GAACTTCTTC ATCTTTGGCA TGCGCGTGGA GGATGTGGAC 
AGGCTCGACC AGAGAGGGTA CAATGCCCAG GAGTACTACG ACCGCATTCC CGAACTGCGG 
CAGGTCATTG ATCAGCTCAG CAGTGGCTTC TTCTCCCCGA AACAGCCCGA CCTGTTCAAG 
GATATTGTCA ACATGCTCAT GCACCACGAC CGGTTTAAAG TCTTTGCCGA TTATGAAGAC 
TACATTAAAT GCCAGGAGAA AGTCAGCGCC CTGTACAAGA ACCCGAGAGA GTGGACACGC 
ATGGTGATTC GGAACATCGC CACCTCGGGC AAGTTCTCCA GCGACCGCAC CATTGCCCAG 
TACGCCCGGG AGATCTGGAA AGTCGAGCCT TCCCGCCAAC GCCTGCCAGC CCCTGATGAG 
ACCATCTGA