LOC100700589 cDNA ORF clone, Oreochromis niloticus(Nile tilapia)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100700589
Entrez Gene ID	100700589
Full Name	GC-rich sequence DNA-binding factor 2
Gene Type	protein-coding
Organism	Oreochromis niloticus(Nile tilapia)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003454494.4	XP_003454542.1	GC-rich sequence DNA-binding factor 2
XM_003454494.5	XP_003454542.1	GC-rich sequence DNA-binding factor 2

Pathways from BioSystems
Gene Ontology

The following LOC100700589 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100700589 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OOr29239	XM_003454494.5 Latest version!	Oreochromis niloticus GC-rich sequence DNA-binding factor 2 (LOC100700589), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
View Old Accession Versions >>
OOr29239	XM_003454494.4	Oreochromis niloticus GC-rich sequence DNA-binding factor 2 (LOC100700589), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100700589 ( XM_003454494.4 ) cDNA ORF clone, Oreochromis niloticus(Nile tilapia) -> XP_003454542.1 Oreochromis niloticus GC-rich sequence DNA-binding factor 2 (LOC100700589), mRNA.

Price & Availability↑

CloneID	OOr29239
Clone ID Related Accession (Same CDS sequence)	XM_003454494.4 , XM_003454494.5
Accession Version	XM_003454494.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2640bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-04
Organism	Oreochromis niloticus(Nile tilapia)
Product	GC-rich sequence DNA-binding factor 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031965.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 5, 2016 this sequence version replaced XM_003454494.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oreochromis niloticus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAACA AGAAACCCCG GAGAAACTTT CGGCAAAGAA AAGACTGCTC CAGCGACGAT 
GAGGACCAGC CAAAGAAGAG CGAAGAAGGA GAGGAAAACC CGGAGAAAAC CGCTCCAGCT 
GTGGTAAATA AGCCCCGTAA AGCCGCGCAG AGCCGCGGCA TCACTTGCAG CTCCAAGCGG 
GAAGCGACGC CTCCGAAGCC GGATACCAGT GACGGAGAAG ACGGGGAGAG CGTGGAAACG 
ACAGAAGAAA GAGAAGAGAG AAAGAAAGAC AAGGAGGGGA CCAAAAAGAA AGAAACCTCT 
GTTTTGAGTT TCTCCGATGA TAAGGAATCT GAGGAGTCTA CCTTTAAATT GAAGAAGTCA 
TCTGAGAAAG CAGTTCTGTT CCAAATTAAG AAGAAGGAGG CTCCGCCTGC CAAGACCATC 
ATCAGCACAG GTGCTGGTGG TGCTGTCTCA TCATCTCCTT CACCCAGGCA AGATTATAGC 
AGTCAGGCTT CACCACATTC TGTGGATGAT GATGACGAAG ATGATGATGA TGATGGTGAT 
GACAATGACA ACAAGGATAA TGGGAGCAAT GATGACAGTG ATAGCGATGA ACGTGGAGTG 
GGATCTCCTT CAGCCAGCTC AGCCTCAGAC TCTAGCAGTT CTGCTTATAC GCCGGTAAAT 
ATCCCCAATG CTAGAGAGAT CGAGGCAGCC AAGAGACGGC GTCGTGCCAA TCGAACCCAG 
AAAGAATTCA TTCCCCTCAG CAGAGATGGC CAGAGTTCGG CTGGCAGCAC CCCAGATCAC 
TACAGCAGGG AGGATGAAGA CGACAGAGTC GATGATGATA ATGAGCTGGA TGATCACGAG 
AGGAGAATCG AGTTTGCCCC ACGGATGAGG AGCATCAGGG AAAGAATTGC TGAGAAACTT 
GGAGAAAGTG ATGGCAGTCG GTCAGGGACT GATGGGGAGG AGCAGGAACT CTGGGAAGAG 
ACACAAATTG GGAAGGGAGT CAAGAGACGA CCAGGGGAGC AGAGCCCATC TGGCAGTGAC 
TCCAGCAGCT ACAGCAGCAG TAGTATAAGC AGGCGAGACA GAGGAAGACA GAAAAGGAAA 
TCTGCAGGAG TCAAAGTTCC AAAGATGCTT CCTCCTGTCA CTGTCTCAAC GGTCAAGAGA 
AGGATCGCTG GAAAACTTGA GTCATTGAAG GAGGTGTACA GAGCACGCCA GGCAGAGCTC 
AGAAGGATGG AGGGCGATGT TGAGGGTGCT AAAACCTCGT TGGAGAATCT GGAGGAAAGC 
TCCTCAGAGA AGCAGCTAAA GTTTTACAGG ACCATGACGA CTTATGTCCA CAACATGGTG 
GAGTGTTTGC AGGAGAAGGT TGTTGAGATC AACTCTTTGG AGCTGGAGTT GCACACTCTG 
CTGTCTGACC AGATGGAGGC GCTGTTGGCT CAGAGACGAG AGAAGATTAA GGAGCAGGCA 
GACCACCTGC AGCAGCTTAG CTATAACACA GCAGAGCAGA GTGCAAGTTC AGCCAATGGA 
TCAGAAACTC AGTGTGAGGT AAGCGTCGGT GGTAAAACTG AGGAAGACTT TGATATGCCT 
GAAGACACAC AACCTTCAGC AGAAGAAGAG GAGCAGCTGC AGAAGAAAAT AGCTGACATA 
CTGTTGAGGT CTAAGGCGGT ATTTTCTGAT GTCCAAGAAG ACTTCTGCAA CGTTAAAAAG 
ATTCTGTCTC GCTTTGAAGA ATGGAGGGAA TGCTACTCGG AGTCCTACCA CAATGCTTAT 
ATCTCTCTGT GTCTGCCCAA GTTGCTGAAC CCCATCATCA GGCATCAGCT GCTGGCGTGG 
AACCCTTTAA AAGATACCAG TGGAGACTTT GAAAACCTCC CGTGGTTCAC AGCAGTGGAG 
ACCTTTTGTC ATGGACATGG CCACGAAGAG CTGGAGCACA CAGACAGACA GACGCTGTCC 
AGCGTCATAG AAAGGACCGT TGTACCCAAA ATGACTGCCT ATGTGGAGCT GGTGTGGGAT 
CCCATGTCCC ACCAACAGTC TGTCTGTCTG ACTGATGTGT GCCACAGTCT GAAGGAGGAC 
TATTCTATCT TTGAAGGAGA GCATACTAAA CCAGTCAAGG CATTCACAGA GGCTCTGGTT 
CGCAGGCTGA GAAGCTGTGT AGATGAAGAT GTCTTTATTC CCCTGTACCC CAAAAAATTT 
CTTGAAGAGG CGTCTTCACC TCAGCGTCAC TTCAGAGATC AGCAGTTCTG GACAGCTGTA 
AAACTCCTAG GCAACATGGG GAAGTGGGAT TTACTGCTCC CTGAGTCTGT ATTGAAAGAA 
CTGATGCTGG ACAAGCTGTT GAACCGATAC CTGATGACCA CACTGTGCAG TCAGACGCTG 
TCCAACAATG CTGTTTATGC ATGCAAAAAG ATAGCAGATG GTCTACCCCC GTCTTGGTTT 
GAAGGGGAGA GCACCTGTTT GCCTCAGCTC CAGAATTTCA GAAACCACAT AGTTCAGAAA 
GTTCATGCCA TCTGTAAACA GCAGCCTCCC AAAGATCCAA ACACCAGGTC TGCTGTGGTT 
GACCTACTGA AGGTTTTGAG TGCAATCAGA TGCCACGACT CCATCATGGC CATAGCAGAA 
AAATACCACT ATGAAGATGC CATCTACTCT CATCAGCTGC TGAACCCAGA GACTGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003454542.1
CDS	82..2721
Translation	MFNKKPRRNFRQRKDCSSDDEDQPKKSEEGEENPEKTAPAVVNKPRKAAQSRGITCSSKREATPPKPDTSDGEDGESVETTEEREERKKDKEGTKKKETSVLSFSDDKESEESTFKLKKSSEKAVLFQIKKKEAPPAKTIISTGAGGAVSSSPSPRQDYSSQASPHSVDDDDEDDDDDGDDNDNKDNGSNDDSDSDERGVGSPSASSASDSSSSAYTPVNIPNAREIEAAKRRRRANRTQKEFIPLSRDGQSSAGSTPDHYSREDEDDRVDDDNELDDHERRIEFAPRMRSIRERIAEKLGESDGSRSGTDGEEQELWEETQIGKGVKRRPGEQSPSGSDSSSYSSSSISRRDRGRQKRKSAGVKVPKMLPPVTVSTVKRRIAGKLESLKEVYRARQAELRRMEGDVEGAKTSLENLEESSSEKQLKFYRTMTTYVHNMVECLQEKVVEINSLELELHTLLSDQMEALLAQRREKIKEQADHLQQLSYNTAEQSASSANGSETQCEVSVGGKTEEDFDMPEDTQPSAEEEEQLQKKIADILLRSKAVFSDVQEDFCNVKKILSRFEEWRECYSESYHNAYISLCLPKLLNPIIRHQLLAWNPLKDTSGDFENLPWFTAVETFCHGHGHEELEHTDRQTLSSVIERTVVPKMTAYVELVWDPMSHQQSVCLTDVCHSLKEDYSIFEGEHTKPVKAFTEALVRRLRSCVDEDVFIPLYPKKFLEEASSPQRHFRDQQFWTAVKLLGNMGKWDLLLPESVLKELMLDKLLNRYLMTTLCSQTLSNNAVYACKKIADGLPPSWFEGESTCLPQLQNFRNHIVQKVHAICKQQPPKDPNTRSAVVDLLKVLSAIRCHDSIMAIAEKYHYEDAIYSHQLLNPETA

Target ORF information:

RefSeq Version	XM_003454494.4
Organism	Oreochromis niloticus(Nile tilapia)
Definition	Oreochromis niloticus GC-rich sequence DNA-binding factor 2 (LOC100700589), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003454494.4

ORF Insert Sequence:

ATGTTCAACA AGAAACCCCG GAGAAACTTT CGGCAAAGAA AAGACTGCTC CAGCGACGAT 
GAGGACCAGC CAAAGAAGAG CGAAGAAGGA GAGGAAAACC CGGAGAAAAC CGCTCCAGCT 
GTGGTAAATA AGCCCCGTAA AGCCGCGCAG AGCCGCGGCA TCACTTGCAG CTCCAAGCGG 
GAAGCGACGC CTCCGAAGCC GGATACCAGT GACGGAGAAG ACGGGGAGAG CGTGGAAACG 
ACAGAAGAAA GAGAAGAGAG AAAGAAAGAC AAGGAGGGGA CCAAAAAGAA AGAAACCTCT 
GTTTTGAGTT TCTCCGATGA TAAGGAATCT GAGGAGTCTA CCTTTAAATT GAAGAAGTCA 
TCTGAGAAAG CAGTTCTGTT CCAAATTAAG AAGAAGGAGG CTCCGCCTGC CAAGACCATC 
ATCAGCACAG GTGCTGGTGG TGCTGTCTCA TCATCTCCTT CACCCAGGCA AGATTATAGC 
AGTCAGGCTT CACCACATTC TGTGGATGAT GATGACGAAG ATGATGATGA TGATGGTGAT 
GACAATGACA ACAAGGATAA TGGGAGCAAT GATGACAGTG ATAGCGATGA ACGTGGAGTG 
GGATCTCCTT CAGCCAGCTC AGCCTCAGAC TCTAGCAGTT CTGCTTATAC GCCGGTAAAT 
ATCCCCAATG CTAGAGAGAT CGAGGCAGCC AAGAGACGGC GTCGTGCCAA TCGAACCCAG 
AAAGAATTCA TTCCCCTCAG CAGAGATGGC CAGAGTTCGG CTGGCAGCAC CCCAGATCAC 
TACAGCAGGG AGGATGAAGA CGACAGAGTC GATGATGATA ATGAGCTGGA TGATCACGAG 
AGGAGAATCG AGTTTGCCCC ACGGATGAGG AGCATCAGGG AAAGAATTGC TGAGAAACTT 
GGAGAAAGTG ATGGCAGTCG GTCAGGGACT GATGGGGAGG AGCAGGAACT CTGGGAAGAG 
ACACAAATTG GGAAGGGAGT CAAGAGACGA CCAGGGGAGC AGAGCCCATC TGGCAGTGAC 
TCCAGCAGCT ACAGCAGCAG TAGTATAAGC AGGCGAGACA GAGGAAGACA GAAAAGGAAA 
TCTGCAGGAG TCAAAGTTCC AAAGATGCTT CCTCCTGTCA CTGTCTCAAC GGTCAAGAGA 
AGGATCGCTG GAAAACTTGA GTCATTGAAG GAGGTGTACA GAGCACGCCA GGCAGAGCTC 
AGAAGGATGG AGGGCGATGT TGAGGGTGCT AAAACCTCGT TGGAGAATCT GGAGGAAAGC 
TCCTCAGAGA AGCAGCTAAA GTTTTACAGG ACCATGACGA CTTATGTCCA CAACATGGTG 
GAGTGTTTGC AGGAGAAGGT TGTTGAGATC AACTCTTTGG AGCTGGAGTT GCACACTCTG 
CTGTCTGACC AGATGGAGGC GCTGTTGGCT CAGAGACGAG AGAAGATTAA GGAGCAGGCA 
GACCACCTGC AGCAGCTTAG CTATAACACA GCAGAGCAGA GTGCAAGTTC AGCCAATGGA 
TCAGAAACTC AGTGTGAGGT AAGCGTCGGT GGTAAAACTG AGGAAGACTT TGATATGCCT 
GAAGACACAC AACCTTCAGC AGAAGAAGAG GAGCAGCTGC AGAAGAAAAT AGCTGACATA 
CTGTTGAGGT CTAAGGCGGT ATTTTCTGAT GTCCAAGAAG ACTTCTGCAA CGTTAAAAAG 
ATTCTGTCTC GCTTTGAAGA ATGGAGGGAA TGCTACTCGG AGTCCTACCA CAATGCTTAT 
ATCTCTCTGT GTCTGCCCAA GTTGCTGAAC CCCATCATCA GGCATCAGCT GCTGGCGTGG 
AACCCTTTAA AAGATACCAG TGGAGACTTT GAAAACCTCC CGTGGTTCAC AGCAGTGGAG 
ACCTTTTGTC ATGGACATGG CCACGAAGAG CTGGAGCACA CAGACAGACA GACGCTGTCC 
AGCGTCATAG AAAGGACCGT TGTACCCAAA ATGACTGCCT ATGTGGAGCT GGTGTGGGAT 
CCCATGTCCC ACCAACAGTC TGTCTGTCTG ACTGATGTGT GCCACAGTCT GAAGGAGGAC 
TATTCTATCT TTGAAGGAGA GCATACTAAA CCAGTCAAGG CATTCACAGA GGCTCTGGTT 
CGCAGGCTGA GAAGCTGTGT AGATGAAGAT GTCTTTATTC CCCTGTACCC CAAAAAATTT 
CTTGAAGAGG CGTCTTCACC TCAGCGTCAC TTCAGAGATC AGCAGTTCTG GACAGCTGTA 
AAACTCCTAG GCAACATGGG GAAGTGGGAT TTACTGCTCC CTGAGTCTGT ATTGAAAGAA 
CTGATGCTGG ACAAGCTGTT GAACCGATAC CTGATGACCA CACTGTGCAG TCAGACGCTG 
TCCAACAATG CTGTTTATGC ATGCAAAAAG ATAGCAGATG GTCTACCCCC GTCTTGGTTT 
GAAGGGGAGA GCACCTGTTT GCCTCAGCTC CAGAATTTCA GAAACCACAT AGTTCAGAAA 
GTTCATGCCA TCTGTAAACA GCAGCCTCCC AAAGATCCAA ACACCAGGTC TGCTGTGGTT 
GACCTACTGA AGGTTTTGAG TGCAATCAGA TGCCACGACT CCATCATGGC CATAGCAGAA 
AAATACCACT ATGAAGATGC CATCTACTCT CATCAGCTGC TGAACCCAGA GACTGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100700589 ( XM_003454494.5 ) cDNA ORF clone, Oreochromis niloticus(Nile tilapia) -> XP_003454542.1 Oreochromis niloticus GC-rich sequence DNA-binding factor 2 (LOC100700589), mRNA.

Price & Availability↑

CloneID	OOr29239
Clone ID Related Accession (Same CDS sequence)	XM_003454494.4 , XM_003454494.5
Accession Version	XM_003454494.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2640bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-07-25
Organism	Oreochromis niloticus(Nile tilapia)
Product	GC-rich sequence DNA-binding factor 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031965.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2018 this sequence version replaced XM_003454494.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oreochromis niloticus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAACA AGAAACCCCG GAGAAACTTT CGGCAAAGAA AAGACTGCTC CAGCGACGAT 
GAGGACCAGC CAAAGAAGAG CGAAGAAGGA GAGGAAAACC CGGAGAAAAC CGCTCCAGCT 
GTGGTAAATA AGCCCCGTAA AGCCGCGCAG AGCCGCGGCA TCACTTGCAG CTCCAAGCGG 
GAAGCGACGC CTCCGAAGCC GGATACCAGT GACGGAGAAG ACGGGGAGAG CGTGGAAACG 
ACAGAAGAAA GAGAAGAGAG AAAGAAAGAC AAGGAGGGGA CCAAAAAGAA AGAAACCTCT 
GTTTTGAGTT TCTCCGATGA TAAGGAATCT GAGGAGTCTA CCTTTAAATT GAAGAAGTCA 
TCTGAGAAAG CAGTTCTGTT CCAAATTAAG AAGAAGGAGG CTCCGCCTGC CAAGACCATC 
ATCAGCACAG GTGCTGGTGG TGCTGTCTCA TCATCTCCTT CACCCAGGCA AGATTATAGC 
AGTCAGGCTT CACCACATTC TGTGGATGAT GATGACGAAG ATGATGATGA TGATGGTGAT 
GACAATGACA ACAAGGATAA TGGGAGCAAT GATGACAGTG ATAGCGATGA ACGTGGAGTG 
GGATCTCCTT CAGCCAGCTC AGCCTCAGAC TCTAGCAGTT CTGCTTATAC GCCGGTAAAT 
ATCCCCAATG CTAGAGAGAT CGAGGCAGCC AAGAGACGGC GTCGTGCCAA TCGAACCCAG 
AAAGAATTCA TTCCCCTCAG CAGAGATGGC CAGAGTTCGG CTGGCAGCAC CCCAGATCAC 
TACAGCAGGG AGGATGAAGA CGACAGAGTC GATGATGATA ATGAGCTGGA TGATCACGAG 
AGGAGAATCG AGTTTGCCCC ACGGATGAGG AGCATCAGGG AAAGAATTGC TGAGAAACTT 
GGAGAAAGTG ATGGCAGTCG GTCAGGGACT GATGGGGAGG AGCAGGAACT CTGGGAAGAG 
ACACAAATTG GGAAGGGAGT CAAGAGACGA CCAGGGGAGC AGAGCCCATC TGGCAGTGAC 
TCCAGCAGCT ACAGCAGCAG TAGTATAAGC AGGCGAGACA GAGGAAGACA GAAAAGGAAA 
TCTGCAGGAG TCAAAGTTCC AAAGATGCTT CCTCCTGTCA CTGTCTCAAC GGTCAAGAGA 
AGGATCGCTG GAAAACTTGA GTCATTGAAG GAGGTGTACA GAGCACGCCA GGCAGAGCTC 
AGAAGGATGG AGGGCGATGT TGAGGGTGCT AAAACCTCGT TGGAGAATCT GGAGGAAAGC 
TCCTCAGAGA AGCAGCTAAA GTTTTACAGG ACCATGACGA CTTATGTCCA CAACATGGTG 
GAGTGTTTGC AGGAGAAGGT TGTTGAGATC AACTCTTTGG AGCTGGAGTT GCACACTCTG 
CTGTCTGACC AGATGGAGGC GCTGTTGGCT CAGAGACGAG AGAAGATTAA GGAGCAGGCA 
GACCACCTGC AGCAGCTTAG CTATAACACA GCAGAGCAGA GTGCAAGTTC AGCCAATGGA 
TCAGAAACTC AGTGTGAGGT AAGCGTCGGT GGTAAAACTG AGGAAGACTT TGATATGCCT 
GAAGACACAC AACCTTCAGC AGAAGAAGAG GAGCAGCTGC AGAAGAAAAT AGCTGACATA 
CTGTTGAGGT CTAAGGCGGT ATTTTCTGAT GTCCAAGAAG ACTTCTGCAA CGTTAAAAAG 
ATTCTGTCTC GCTTTGAAGA ATGGAGGGAA TGCTACTCGG AGTCCTACCA CAATGCTTAT 
ATCTCTCTGT GTCTGCCCAA GTTGCTGAAC CCCATCATCA GGCATCAGCT GCTGGCGTGG 
AACCCTTTAA AAGATACCAG TGGAGACTTT GAAAACCTCC CGTGGTTCAC AGCAGTGGAG 
ACCTTTTGTC ATGGACATGG CCACGAAGAG CTGGAGCACA CAGACAGACA GACGCTGTCC 
AGCGTCATAG AAAGGACCGT TGTACCCAAA ATGACTGCCT ATGTGGAGCT GGTGTGGGAT 
CCCATGTCCC ACCAACAGTC TGTCTGTCTG ACTGATGTGT GCCACAGTCT GAAGGAGGAC 
TATTCTATCT TTGAAGGAGA GCATACTAAA CCAGTCAAGG CATTCACAGA GGCTCTGGTT 
CGCAGGCTGA GAAGCTGTGT AGATGAAGAT GTCTTTATTC CCCTGTACCC CAAAAAATTT 
CTTGAAGAGG CGTCTTCACC TCAGCGTCAC TTCAGAGATC AGCAGTTCTG GACAGCTGTA 
AAACTCCTAG GCAACATGGG GAAGTGGGAT TTACTGCTCC CTGAGTCTGT ATTGAAAGAA 
CTGATGCTGG ACAAGCTGTT GAACCGATAC CTGATGACCA CACTGTGCAG TCAGACGCTG 
TCCAACAATG CTGTTTATGC ATGCAAAAAG ATAGCAGATG GTCTACCCCC GTCTTGGTTT 
GAAGGGGAGA GCACCTGTTT GCCTCAGCTC CAGAATTTCA GAAACCACAT AGTTCAGAAA 
GTTCATGCCA TCTGTAAACA GCAGCCTCCC AAAGATCCAA ACACCAGGTC TGCTGTGGTT 
GACCTACTGA AGGTTTTGAG TGCAATCAGA TGCCACGACT CCATCATGGC CATAGCAGAA 
AAATACCACT ATGAAGATGC CATCTACTCT CATCAGCTGC TGAACCCAGA GACTGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003454542.1
CDS	75..2714
Translation	MFNKKPRRNFRQRKDCSSDDEDQPKKSEEGEENPEKTAPAVVNKPRKAAQSRGITCSSKREATPPKPDTSDGEDGESVETTEEREERKKDKEGTKKKETSVLSFSDDKESEESTFKLKKSSEKAVLFQIKKKEAPPAKTIISTGAGGAVSSSPSPRQDYSSQASPHSVDDDDEDDDDDGDDNDNKDNGSNDDSDSDERGVGSPSASSASDSSSSAYTPVNIPNAREIEAAKRRRRANRTQKEFIPLSRDGQSSAGSTPDHYSREDEDDRVDDDNELDDHERRIEFAPRMRSIRERIAEKLGESDGSRSGTDGEEQELWEETQIGKGVKRRPGEQSPSGSDSSSYSSSSISRRDRGRQKRKSAGVKVPKMLPPVTVSTVKRRIAGKLESLKEVYRARQAELRRMEGDVEGAKTSLENLEESSSEKQLKFYRTMTTYVHNMVECLQEKVVEINSLELELHTLLSDQMEALLAQRREKIKEQADHLQQLSYNTAEQSASSANGSETQCEVSVGGKTEEDFDMPEDTQPSAEEEEQLQKKIADILLRSKAVFSDVQEDFCNVKKILSRFEEWRECYSESYHNAYISLCLPKLLNPIIRHQLLAWNPLKDTSGDFENLPWFTAVETFCHGHGHEELEHTDRQTLSSVIERTVVPKMTAYVELVWDPMSHQQSVCLTDVCHSLKEDYSIFEGEHTKPVKAFTEALVRRLRSCVDEDVFIPLYPKKFLEEASSPQRHFRDQQFWTAVKLLGNMGKWDLLLPESVLKELMLDKLLNRYLMTTLCSQTLSNNAVYACKKIADGLPPSWFEGESTCLPQLQNFRNHIVQKVHAICKQQPPKDPNTRSAVVDLLKVLSAIRCHDSIMAIAEKYHYEDAIYSHQLLNPETA

Target ORF information:

RefSeq Version	XM_003454494.5
Organism	Oreochromis niloticus(Nile tilapia)
Definition	Oreochromis niloticus GC-rich sequence DNA-binding factor 2 (LOC100700589), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003454494.5

ORF Insert Sequence:

ATGTTCAACA AGAAACCCCG GAGAAACTTT CGGCAAAGAA AAGACTGCTC CAGCGACGAT 
GAGGACCAGC CAAAGAAGAG CGAAGAAGGA GAGGAAAACC CGGAGAAAAC CGCTCCAGCT 
GTGGTAAATA AGCCCCGTAA AGCCGCGCAG AGCCGCGGCA TCACTTGCAG CTCCAAGCGG 
GAAGCGACGC CTCCGAAGCC GGATACCAGT GACGGAGAAG ACGGGGAGAG CGTGGAAACG 
ACAGAAGAAA GAGAAGAGAG AAAGAAAGAC AAGGAGGGGA CCAAAAAGAA AGAAACCTCT 
GTTTTGAGTT TCTCCGATGA TAAGGAATCT GAGGAGTCTA CCTTTAAATT GAAGAAGTCA 
TCTGAGAAAG CAGTTCTGTT CCAAATTAAG AAGAAGGAGG CTCCGCCTGC CAAGACCATC 
ATCAGCACAG GTGCTGGTGG TGCTGTCTCA TCATCTCCTT CACCCAGGCA AGATTATAGC 
AGTCAGGCTT CACCACATTC TGTGGATGAT GATGACGAAG ATGATGATGA TGATGGTGAT 
GACAATGACA ACAAGGATAA TGGGAGCAAT GATGACAGTG ATAGCGATGA ACGTGGAGTG 
GGATCTCCTT CAGCCAGCTC AGCCTCAGAC TCTAGCAGTT CTGCTTATAC GCCGGTAAAT 
ATCCCCAATG CTAGAGAGAT CGAGGCAGCC AAGAGACGGC GTCGTGCCAA TCGAACCCAG 
AAAGAATTCA TTCCCCTCAG CAGAGATGGC CAGAGTTCGG CTGGCAGCAC CCCAGATCAC 
TACAGCAGGG AGGATGAAGA CGACAGAGTC GATGATGATA ATGAGCTGGA TGATCACGAG 
AGGAGAATCG AGTTTGCCCC ACGGATGAGG AGCATCAGGG AAAGAATTGC TGAGAAACTT 
GGAGAAAGTG ATGGCAGTCG GTCAGGGACT GATGGGGAGG AGCAGGAACT CTGGGAAGAG 
ACACAAATTG GGAAGGGAGT CAAGAGACGA CCAGGGGAGC AGAGCCCATC TGGCAGTGAC 
TCCAGCAGCT ACAGCAGCAG TAGTATAAGC AGGCGAGACA GAGGAAGACA GAAAAGGAAA 
TCTGCAGGAG TCAAAGTTCC AAAGATGCTT CCTCCTGTCA CTGTCTCAAC GGTCAAGAGA 
AGGATCGCTG GAAAACTTGA GTCATTGAAG GAGGTGTACA GAGCACGCCA GGCAGAGCTC 
AGAAGGATGG AGGGCGATGT TGAGGGTGCT AAAACCTCGT TGGAGAATCT GGAGGAAAGC 
TCCTCAGAGA AGCAGCTAAA GTTTTACAGG ACCATGACGA CTTATGTCCA CAACATGGTG 
GAGTGTTTGC AGGAGAAGGT TGTTGAGATC AACTCTTTGG AGCTGGAGTT GCACACTCTG 
CTGTCTGACC AGATGGAGGC GCTGTTGGCT CAGAGACGAG AGAAGATTAA GGAGCAGGCA 
GACCACCTGC AGCAGCTTAG CTATAACACA GCAGAGCAGA GTGCAAGTTC AGCCAATGGA 
TCAGAAACTC AGTGTGAGGT AAGCGTCGGT GGTAAAACTG AGGAAGACTT TGATATGCCT 
GAAGACACAC AACCTTCAGC AGAAGAAGAG GAGCAGCTGC AGAAGAAAAT AGCTGACATA 
CTGTTGAGGT CTAAGGCGGT ATTTTCTGAT GTCCAAGAAG ACTTCTGCAA CGTTAAAAAG 
ATTCTGTCTC GCTTTGAAGA ATGGAGGGAA TGCTACTCGG AGTCCTACCA CAATGCTTAT 
ATCTCTCTGT GTCTGCCCAA GTTGCTGAAC CCCATCATCA GGCATCAGCT GCTGGCGTGG 
AACCCTTTAA AAGATACCAG TGGAGACTTT GAAAACCTCC CGTGGTTCAC AGCAGTGGAG 
ACCTTTTGTC ATGGACATGG CCACGAAGAG CTGGAGCACA CAGACAGACA GACGCTGTCC 
AGCGTCATAG AAAGGACCGT TGTACCCAAA ATGACTGCCT ATGTGGAGCT GGTGTGGGAT 
CCCATGTCCC ACCAACAGTC TGTCTGTCTG ACTGATGTGT GCCACAGTCT GAAGGAGGAC 
TATTCTATCT TTGAAGGAGA GCATACTAAA CCAGTCAAGG CATTCACAGA GGCTCTGGTT 
CGCAGGCTGA GAAGCTGTGT AGATGAAGAT GTCTTTATTC CCCTGTACCC CAAAAAATTT 
CTTGAAGAGG CGTCTTCACC TCAGCGTCAC TTCAGAGATC AGCAGTTCTG GACAGCTGTA 
AAACTCCTAG GCAACATGGG GAAGTGGGAT TTACTGCTCC CTGAGTCTGT ATTGAAAGAA 
CTGATGCTGG ACAAGCTGTT GAACCGATAC CTGATGACCA CACTGTGCAG TCAGACGCTG 
TCCAACAATG CTGTTTATGC ATGCAAAAAG ATAGCAGATG GTCTACCCCC GTCTTGGTTT 
GAAGGGGAGA GCACCTGTTT GCCTCAGCTC CAGAATTTCA GAAACCACAT AGTTCAGAAA 
GTTCATGCCA TCTGTAAACA GCAGCCTCCC AAAGATCCAA ACACCAGGTC TGCTGTGGTT 
GACCTACTGA AGGTTTTGAG TGCAATCAGA TGCCACGACT CCATCATGGC CATAGCAGAA 
AAATACCACT ATGAAGATGC CATCTACTCT CATCAGCTGC TGAACCCAGA GACTGCATGA