LOC100692532 cDNA ORF clone, Oreochromis niloticus(Nile tilapia)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100692532
Entrez Gene ID	100692532
Full Name	prospero homeobox protein 1
Gene Type	protein-coding
Organism	Oreochromis niloticus(Nile tilapia)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003446607.5	XP_003446655.3	LOW QUALITY PROTEIN: prospero homeobox protein 1
XM_003446607.4	XP_003446655.2	LOW QUALITY PROTEIN: prospero homeobox protein 1

Pathways from BioSystems
Gene Ontology

The following LOC100692532 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100692532 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OOr180336	XM_003446607.5 Latest version!	Oreochromis niloticus prospero homeobox protein 1 (LOC100692532), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OOr56992	XM_003446607.4 Latest version!	Oreochromis niloticus prospero homeobox protein 1 (LOC100692532), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100692532 ( XM_003446607.5 ) cDNA ORF clone, Oreochromis niloticus(Nile tilapia) -> XP_003446655.3 Oreochromis niloticus prospero homeobox protein 1 (LOC100692532), mRNA.

Price & Availability↑

CloneID	OOr180336
Clone ID Related Accession (Same CDS sequence)	XM_003446607.5
Accession Version	XM_003446607.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2694bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-07-25
Organism	Oreochromis niloticus(Nile tilapia)
Product	LOW QUALITY PROTEIN: prospero homeobox protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031986.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2018 this sequence version replaced XM_003446607.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oreochromis niloticus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGCCTGATC ATGACAGCAC CACCCTCCTA ACAAGACAGA CCAAGCGCCG ACGTGTTGAC 
ATCGGGGTGA AAAGGACTGT GGGTAGCGCG ATATTCGCCC GCGCCAGGGA CACCCTGCTC 
GACAGAATGA ACCAATCCCA CGGCCCCGAT CAGGACGGCG ACTGCTCGCT GGTTGGTCAC 
AGCCACGGGG CAACCAACGG TGACGGCGAG AAGTCGAACG TTCTGAGAAA GCTGCTGAAG 
AGGGCGAACT CGTACGAGGA CGCCATAATG CCTTTCCCTG GGGCTACGAT CATCTCTCAG 
CTCTTGAAAA ACAACATGGG GAAGAACGGA GGCAGTGACT CGGGTTTTCA GGGGAGTGGT 
GCTCTGTCCA GTGGAGGCTC AGAGATCCAG GCCGAGGATG CCTGCAGCAA CTCCTCCCGT 
GATCGGGACA GCCCGTCTGA CTGCCTCTCT CCTGGGCCTC CTCTGCCCCC TCCATCTTCC 
TCCTCCTCCT TCGGGCGGCC ACCCCCTCCT ACGAGTCTCA ACTCCCACGC TCCCTCTCAG 
CCCCTCTCCC TTTCCTCCTT CGAATTGGAC CGGCTGTCCG ACGAGCACCT TCGTGCCAAG 
CGCGCCCGTG TGGAAAACAT CATCCGCGGC ATGAGCCATT CTCCGCTGGT CCGACCCAAT 
GCCGGCGACC ACAACCAAGA GAGCAACCGC GAAAATGAGA ACAGCAGCAA CAACAGCAAC 
AGCAGTAACG ACAACAGAGG CGACGGCAAC GGCCACAGCA CCGGCAGTCA CAGCCGGAGC 
GACTGCCCCT CTTCTCTGCT GAGCTCCCCT GGCTCGCGGG GTGGCATGGT TGGAGTCGGT 
GAGGTCTACA GGGAGAATAA GAGAAAGCAG CGCCTGCCAC AGCAGCAGCA CAGCTTCACC 
CAGCTGGTGT GTTCCAGGCA GGAGCAGAGA CAGGAAGAAA GAAGGCAGCT CAAACTACAG 
CTGGAAGACA TGCAGAAACA GCTCCGCCAG CTTCAGGAGA AGTTCTACCA GATCTACGAC 
TCGGAGACTG AGGAGGAGGA AGACAATGGC GAGAACGGTG AGCCTGACCG AGGCGGAGAA 
AGAGAGGAGG ACGGCAACTT GTCAGAGGAC AGCATCCGCT CCGACGGCCT GGAGGAGCGG 
CACAGAGAGA GGGGCCGGCG CGGCCACGAC GACCTCTCCG AGCTGGACCC GGGTCTGTTC 
CTGGACCGGG CACGGGCCCT CCTCCGAGAA CAGGCCTTGA TAGATGGAGA AATGGAGGAG 
GATGTGGACA GCAGGGAGGA CGAGGAGAGG AACGGGGAGA GGGTAATGAA GAGGAGGGGC 
GGTGGGGCAG GGGCCGGAGG AGGAGGAGGA GGAGGGAGGC AGTTAGCCGA GACTCTGAAG 
CAGGAGCTGA ACTGTGCCGT GTCACAGGTT GTCGACACCG TTGTCAAAGG CTTCTCCTCA 
CCCAAACAGG CCATTAATCA CCATCATGGC TGCCACAGCA GCACGCCGGC CGCGCCTTCC 
TCTTCCTCCA ACTCGTCCTC CTCGTGCCCT CCGCCTTTTG GCCCCATGCC CTCCCTCACC 
CCTGACTCTC GATTTGGTGG CGGCGCTTTG GCCCTCAGTC TGAACGGCGA TGGCAGTCCC 
ACTCCTAACT ACCACTCTTC CAACCAGAGG CTGCACTGCT TCGGGGACGT GATTGTCCCG 
AGCCCGCTGG ATTCGTTCGG CGGGTTGAGT GGCAGACTAA GTGGTGTGCC AACGCCCAAC 
GACCAAACAG AAGCACTGCC GCTGGTGGTG CGCAAGAGCG GTGGAGGAGG AGGAGGAGAG 
AACGCCGACC CCTCTCTTCC CCCTCCACCT CCTCCTCACC ATCCCTCTCT CCACCCGACT 
CCCCTCACAG CTTCGCTCGG GTTTAGCCCT CCATCCTTCC GCCACCCGTT CCCTCTTCCC 
CTCATGGGCT ATCCCTTTCA GAGCCCCCTG GGGTCACATG GGGGTGGAGC CGGGTACCCT 
CCGGGGCCCA AGGACCACCA CCGGTCCTCT CCCGATTCGA TGGACATAAC TCGGGAAACC 
AGCCTCAGAA CCAAAATGGC GTCCCTGGCG GGAGGCCATC TTGGTCACCA TCACCACAGG 
CAGAGCTCCC CAAACCAACA CGAGGGTCTG TCGCTATCCC TCATCAAGTC TGAGTGCGGG 
GACCTGCAAG ACATGTCTGA CATCTCGCCC TACTCTGGCA GCACCATTCA AGAAGGACTG 
TCTCCGAACC ACCTGAAGAA GGCCAAGCTG ATGTTCTTCT ACACCCGCTA TCCCTCATCC 
AACATGCTCA AAATGTACTT CTCTGATGTC AAGTTCAATC GATGCATCAC CTCCCAGCTG 
ATCAAGTGGT TCAGCAACTT CAGGGAGTTC TACTACATCC AGATGGAGAA GTTTGCTCGG 
CAGGCCATCA ACGAGGGCGT GACCGCTGCC GAGGAGCTGA CCGTGGGCCG AGATGCCGAA 
CTGTTCAGGG CACTCAACAT GCACTACAAC AAGGCCAACG ACTTTGAGGT TCCTGATCGA 
TTCCTGGAGG TGGCCCAGGT GACCCTTCGT GAGTTCTTCA ATGCCATCGT GGCTGGAAAG 
GATGCGGACC CATCCTGGAA GAAGGCCATC TACAAAGTGA TCTGCAAACT GGACAGCGAG 
GTTCCCGAGG TCTTCAAATC CCCCAACTGT CTACAAGAGC TCCTCCATGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003446655.3
CDS	697..3390
Translation	MPDHDSTTLLTRQTKRRRVDIGVKRTVGSAIFARARDTLLDRMNQSHGPDQDGDCSLVGHSHGATNGDGEKSNVLRKLLKRANSYEDAIMPFPGATIISQLLKNNMGKNGGSDSGFQGSGALSSGGSEIQAEDACSNSSRDRDSPSDCLSPGPPLPPPSSSSSFGRPPPPTSLNSHAPSQPLSLSSFELDRLSDEHLRAKRARVENIIRGMSHSPLVRPNAGDHNQESNRENENSSNNSNSSNDNRGDGNGHSTGSHSRSDCPSSLLSSPGSRGGMVGVGEVYRENKRKQRLPQQQHSFTQLVCSRQEQRQEERRQLKLQLEDMQKQLRQLQEKFYQIYDSETEEEEDNGENGEPDRGGEREEDGNLSEDSIRSDGLEERHRERGRRGHDDLSELDPGLFLDRARALLREQALIDGEMEEDVDSREDEERNGERVMKRRGGGAGAGGGGGGGRQLAETLKQELNCAVSQVVDTVVKGFSSPKQAINHHHGCHSSTPAAPSSSSNSSSSCPPPFGPMPSLTPDSRFGGGALALSLNGDGSPTPNYHSSNQRLHCFGDVIVPSPLDSFGGLSGRLSGVPTPNDQTEALPLVVRKSGGGGGGENADPSLPPPPPPHHPSLHPTPLTASLGFSPPSFRHPFPLPLMGYPFQSPLGSHGGGAGYPPGPKDHHRSSPDSMDITRETSLRTKMASLAGGHLGHHHHRQSSPNQHEGLSLSLIKSECGDLQDMSDISPYSGSTIQEGLSPNHLKKAKLMFFYTRYPSSNMLKMYFSDVKFNRCITSQLIKWFSNFREFYYIQMEKFARQAINEGVTAAEELTVGRDAELFRALNMHYNKANDFEVPDRFLEVAQVTLREFFNAIVAGKDADPSWKKAIYKVICKLDSEVPEVFKSPNCLQELLHD

Target ORF information:

RefSeq Version	XM_003446607.5
Organism	Oreochromis niloticus(Nile tilapia)
Definition	Oreochromis niloticus prospero homeobox protein 1 (LOC100692532), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003446607.5

ORF Insert Sequence:

ATGCCTGATC ATGACAGCAC CACCCTCCTA ACAAGACAGA CCAAGCGCCG ACGTGTTGAC 
ATCGGGGTGA AAAGGACTGT GGGTAGCGCG ATATTCGCCC GCGCCAGGGA CACCCTGCTC 
GACAGAATGA ACCAATCCCA CGGCCCCGAT CAGGACGGCG ACTGCTCGCT GGTTGGTCAC 
AGCCACGGGG CAACCAACGG TGACGGCGAG AAGTCGAACG TTCTGAGAAA GCTGCTGAAG 
AGGGCGAACT CGTACGAGGA CGCCATAATG CCTTTCCCTG GGGCTACGAT CATCTCTCAG 
CTCTTGAAAA ACAACATGGG GAAGAACGGA GGCAGTGACT CGGGTTTTCA GGGGAGTGGT 
GCTCTGTCCA GTGGAGGCTC AGAGATCCAG GCCGAGGATG CCTGCAGCAA CTCCTCCCGT 
GATCGGGACA GCCCGTCTGA CTGCCTCTCT CCTGGGCCTC CTCTGCCCCC TCCATCTTCC 
TCCTCCTCCT TCGGGCGGCC ACCCCCTCCT ACGAGTCTCA ACTCCCACGC TCCCTCTCAG 
CCCCTCTCCC TTTCCTCCTT CGAATTGGAC CGGCTGTCCG ACGAGCACCT TCGTGCCAAG 
CGCGCCCGTG TGGAAAACAT CATCCGCGGC ATGAGCCATT CTCCGCTGGT CCGACCCAAT 
GCCGGCGACC ACAACCAAGA GAGCAACCGC GAAAATGAGA ACAGCAGCAA CAACAGCAAC 
AGCAGTAACG ACAACAGAGG CGACGGCAAC GGCCACAGCA CCGGCAGTCA CAGCCGGAGC 
GACTGCCCCT CTTCTCTGCT GAGCTCCCCT GGCTCGCGGG GTGGCATGGT TGGAGTCGGT 
GAGGTCTACA GGGAGAATAA GAGAAAGCAG CGCCTGCCAC AGCAGCAGCA CAGCTTCACC 
CAGCTGGTGT GTTCCAGGCA GGAGCAGAGA CAGGAAGAAA GAAGGCAGCT CAAACTACAG 
CTGGAAGACA TGCAGAAACA GCTCCGCCAG CTTCAGGAGA AGTTCTACCA GATCTACGAC 
TCGGAGACTG AGGAGGAGGA AGACAATGGC GAGAACGGTG AGCCTGACCG AGGCGGAGAA 
AGAGAGGAGG ACGGCAACTT GTCAGAGGAC AGCATCCGCT CCGACGGCCT GGAGGAGCGG 
CACAGAGAGA GGGGCCGGCG CGGCCACGAC GACCTCTCCG AGCTGGACCC GGGTCTGTTC 
CTGGACCGGG CACGGGCCCT CCTCCGAGAA CAGGCCTTGA TAGATGGAGA AATGGAGGAG 
GATGTGGACA GCAGGGAGGA CGAGGAGAGG AACGGGGAGA GGGTAATGAA GAGGAGGGGC 
GGTGGGGCAG GGGCCGGAGG AGGAGGAGGA GGAGGGAGGC AGTTAGCCGA GACTCTGAAG 
CAGGAGCTGA ACTGTGCCGT GTCACAGGTT GTCGACACCG TTGTCAAAGG CTTCTCCTCA 
CCCAAACAGG CCATTAATCA CCATCATGGC TGCCACAGCA GCACGCCGGC CGCGCCTTCC 
TCTTCCTCCA ACTCGTCCTC CTCGTGCCCT CCGCCTTTTG GCCCCATGCC CTCCCTCACC 
CCTGACTCTC GATTTGGTGG CGGCGCTTTG GCCCTCAGTC TGAACGGCGA TGGCAGTCCC 
ACTCCTAACT ACCACTCTTC CAACCAGAGG CTGCACTGCT TCGGGGACGT GATTGTCCCG 
AGCCCGCTGG ATTCGTTCGG CGGGTTGAGT GGCAGACTAA GTGGTGTGCC AACGCCCAAC 
GACCAAACAG AAGCACTGCC GCTGGTGGTG CGCAAGAGCG GTGGAGGAGG AGGAGGAGAG 
AACGCCGACC CCTCTCTTCC CCCTCCACCT CCTCCTCACC ATCCCTCTCT CCACCCGACT 
CCCCTCACAG CTTCGCTCGG GTTTAGCCCT CCATCCTTCC GCCACCCGTT CCCTCTTCCC 
CTCATGGGCT ATCCCTTTCA GAGCCCCCTG GGGTCACATG GGGGTGGAGC CGGGTACCCT 
CCGGGGCCCA AGGACCACCA CCGGTCCTCT CCCGATTCGA TGGACATAAC TCGGGAAACC 
AGCCTCAGAA CCAAAATGGC GTCCCTGGCG GGAGGCCATC TTGGTCACCA TCACCACAGG 
CAGAGCTCCC CAAACCAACA CGAGGGTCTG TCGCTATCCC TCATCAAGTC TGAGTGCGGG 
GACCTGCAAG ACATGTCTGA CATCTCGCCC TACTCTGGCA GCACCATTCA AGAAGGACTG 
TCTCCGAACC ACCTGAAGAA GGCCAAGCTG ATGTTCTTCT ACACCCGCTA TCCCTCATCC 
AACATGCTCA AAATGTACTT CTCTGATGTC AAGTTCAATC GATGCATCAC CTCCCAGCTG 
ATCAAGTGGT TCAGCAACTT CAGGGAGTTC TACTACATCC AGATGGAGAA GTTTGCTCGG 
CAGGCCATCA ACGAGGGCGT GACCGCTGCC GAGGAGCTGA CCGTGGGCCG AGATGCCGAA 
CTGTTCAGGG CACTCAACAT GCACTACAAC AAGGCCAACG ACTTTGAGGT TCCTGATCGA 
TTCCTGGAGG TGGCCCAGGT GACCCTTCGT GAGTTCTTCA ATGCCATCGT GGCTGGAAAG 
GATGCGGACC CATCCTGGAA GAAGGCCATC TACAAAGTGA TCTGCAAACT GGACAGCGAG 
GTTCCCGAGG TCTTCAAATC CCCCAACTGT CTACAAGAGC TCCTCCATGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100692532 ( XM_003446607.4 ) cDNA ORF clone, Oreochromis niloticus(Nile tilapia) -> XP_003446655.2 Oreochromis niloticus prospero homeobox protein 1 (LOC100692532), mRNA.

Price & Availability↑

CloneID	OOr56992
Clone ID Related Accession (Same CDS sequence)	XM_003446607.4
Accession Version	XM_003446607.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2694bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-04
Organism	Oreochromis niloticus(Nile tilapia)
Product	LOW QUALITY PROTEIN: prospero homeobox protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031986.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 5, 2016 this sequence version replaced XM_003446607.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oreochromis niloticus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGCCTGATC ATGACAGCAC CACCCTCCTA ACAAGACAGA CCAAGCGCCG ACGTGTTGAC 
ATCGGGGTGA AAAGGACTGT GGGTAGCGCG ATATTCGCCC GCGCCAGGGA CACCCTGCTC 
GACAGAATGA ACCAATCCCA CGGCCCCGAT CAGGACGGCG ACTGCTCGCT GGTTGGTCAC 
AGCCACGGGG CAACCAACGG TGACGGCGAG AAGTCGAACG TTCTGAGAAA GCTGCTGAAG 
AGGGCGAACT CGTACGAGGA CGCCATAATG CCTTTCCCTG GGGCTACGAT CATCTCTCAG 
CTCTTGAAAA ACAACATGGG GAAGAACGGA GGCAGTGACT CGGGTTTTCA GGGGAGTGGT 
GCTCTGTCCA GTGGAGGCTC AGAGATCCAG GCCGAGGATG CCTGCAGCAA CTCCTCCCGT 
GATCGGGACA GCCCGTCTGA CTGCCTCTCT CCTGGGCCTC CTCTGCCCCC TCCATCTTCC 
TCCTCCTCCT TCGGGCGGCC ACCCCCTCCT ACGAGTCTCA ACTCCCACGC TCCCTCTCAG 
CCCCTCTCCC TTTCCTCCTT CGAATTGGAC CGGCTGTCCG ACGAGCACCT TCGTGCCAAG 
CGCGCCCGTG TGGAAAACAT CATCCGCGGC ATGAGCCATT CTCCGCTGGT CCGACCCAAT 
GCCGGCGACC ACAACCAAGA GAGCAACCGC GAAAATGAGA ACAGCAGCAA CAACAGCAAC 
AGCAGTAACG ACAACAGAGG CGACGGCAAC GGCCACAGCA CCGGCAGTCA CAGCCGGAGC 
GACTGCCCCT CTTCTCTGCT GAGCTCCCCT GGCTCGCGGG GTGGCATGGT TGGAGTCGGT 
GAGGTCTACA GGGAGAATAA GAGAAAGCAG CGCCTGCCAC AGCAGCAGCA CAGCTTCACC 
CAGCTGGTGT GTTCCAGGCA GGAGCAGAGA CAGGAAGAAA GAAGGCAGCT CAAACTACAG 
CTGGAAGACA TGCAGAAACA GCTCCGCCAG CTTCAGGAGA AGTTCTACCA GATCTACGAC 
TCGGAGACTG AGGAGGAGGA AGACAATGGC GAGAACGGTG AGCCTGACCG AGGCGGAGAA 
AGAGAGGAGG ACGGCAACTT GTCAGAGGAC AGCATCCGCT CCGACGGCCT GGAGGAGCGG 
CACAGAGAGA GGGGCCGGCG CGGCCACGAC GACCTCTCCG AGCTGGACCC GGGTCTGTTC 
CTGGACCGGG CACGGGCCCT CCTCCGAGAA CAGGCCTTGA TAGATGGAGA AATGGAGGAG 
GATGTGGACA GCAGGGAGGA CGAGGAGAGG AACGGGGAGA GGGTAATGAA GAGGAGGGGG 
CGGTGGGGCA GGGCCGGAGG AGGAGGAGGA GGAGGGAGGC AGTTAGCCGA GACTCTGAAG 
CAGGAGCTGA ACTGTGCCGT GTCACAGGTT GTCGACACCG TTGTCAAAGG CTTCTCCTCA 
CCCAAACAGG CCATTAATCA CCATCATGGC TGCCACAGCA GCACGCCGGC CGCGCCTTCC 
TCTTCCTCCA ACTCGTCCTC CTCGTGCCCT CCGCCTTTTG GCCCCATGCC CTCCCTCACC 
CCTGACTCTC GATTTGGTGG CGGCGCTTTG GCCCTCAGTC TGAACGGCGA TGGCAGTCCC 
ACTCCTAACT ACCACTCTTC CAACCAGAGG CTGCACTGCT TCGGGGACGT GATTGTCCCG 
AGCCCGCTGG ATTCGTTCGG CGGGTTGAGT GGCAGACTAA GTGGTGTGCC AACGCCCAAC 
GACCAAACAG AAGCACTGCC GCTGGTGGTG CGCAAGAGCG GTGGAGGAGG AGGAGGAGAG 
AACGCCGACC CCTCTCTTCC CCCTCCACCT CCTCCTCACC ATCCCTCTCT CCACCCGACT 
CCCCTCACAG CTTCGCTCGG GTTTAGCCCT CCATCCTTCC GCCACCCGTT CCCTCTTCCC 
CTCATGGGCT ATCCCTTTCA GAGCCCCCTG GGGTCACATG GGGGTGGAGC CGGGTACCCT 
CCGGGGCCCA AGGACCACCA CCGGTCCTCT CCCGATTCGA TGGACATAAC TCGGGAAACC 
AGCCTCAGAA CCAAAATGGC GTCCCTGGCG GGAGGCCATC TTGGTCACCA TCACCACAGG 
CAGAGCTCCC CAAACCAACA CGAGGGTCTG TCGCTATCCC TCATCAAGTC TGAGTGCGGG 
GACCTGCAAG ACATGTCTGA CATCTCGCCC TACTCTGGCA GCACCATTCA AGAAGGACTG 
TCTCCGAACC ACCTGAAGAA GGCCAAGCTG ATGTTCTTCT ACACCCGCTA TCCCTCATCC 
AACATGCTCA AAATGTACTT CTCTGATGTC AAGTTCAATC GATGCATCAC CTCCCAGCTG 
ATCAAGTGGT TCAGCAACTT CAGGGAGTTC TACTACATCC AGATGGAGAA GTTTGCTCGG 
CAGGCCATCA ACGAGGGCGT GACCGCTGCC GAGGAGCTGA CCGTGGGCCG AGATGCCGAA 
CTGTTCAGGG CACTCAACAT GCACTACAAC AAGGCCAACG ACTTTGAGGT TCCTGATCGA 
TTCCTGGAGG TGGCCCAGGT GACCCTTCGT GAGTTCTTCA ATGCCATCGT GGCTGGAAAG 
GATGCGGACC CATCCTGGAA GAAGGCCATC TACAAAGTGA TCTGCAAACT GGACAGCGAG 
GTTCCCGAGG TCTTCAAATC CCCCAACTGT CTACAAGAGC TCCTCCATGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003446655.2
CDS	668..3361
Translation	MPDHDSTTLLTRQTKRRRVDIGVKRTVGSAIFARARDTLLDRMNQSHGPDQDGDCSLVGHSHGATNGDGEKSNVLRKLLKRANSYEDAIMPFPGATIISQLLKNNMGKNGGSDSGFQGSGALSSGGSEIQAEDACSNSSRDRDSPSDCLSPGPPLPPPSSSSSFGRPPPPTSLNSHAPSQPLSLSSFELDRLSDEHLRAKRARVENIIRGMSHSPLVRPNAGDHNQESNRENENSSNNSNSSNDNRGDGNGHSTGSHSRSDCPSSLLSSPGSRGGMVGVGEVYRENKRKQRLPQQQHSFTQLVCSRQEQRQEERRQLKLQLEDMQKQLRQLQEKFYQIYDSETEEEEDNGENGEPDRGGEREEDGNLSEDSIRSDGLEERHRERGRRGHDDLSELDPGLFLDRARALLREQALIDGEMEEDVDSREDEERNGERVMKRRGRWGRAGGGGGGGRQLAETLKQELNCAVSQVVDTVVKGFSSPKQAINHHHGCHSSTPAAPSSSSNSSSSCPPPFGPMPSLTPDSRFGGGALALSLNGDGSPTPNYHSSNQRLHCFGDVIVPSPLDSFGGLSGRLSGVPTPNDQTEALPLVVRKSGGGGGGENADPSLPPPPPPHHPSLHPTPLTASLGFSPPSFRHPFPLPLMGYPFQSPLGSHGGGAGYPPGPKDHHRSSPDSMDITRETSLRTKMASLAGGHLGHHHHRQSSPNQHEGLSLSLIKSECGDLQDMSDISPYSGSTIQEGLSPNHLKKAKLMFFYTRYPSSNMLKMYFSDVKFNRCITSQLIKWFSNFREFYYIQMEKFARQAINEGVTAAEELTVGRDAELFRALNMHYNKANDFEVPDRFLEVAQVTLREFFNAIVAGKDADPSWKKAIYKVICKLDSEVPEVFKSPNCLQELLHD

Target ORF information:

RefSeq Version	XM_003446607.4
Organism	Oreochromis niloticus(Nile tilapia)
Definition	Oreochromis niloticus prospero homeobox protein 1 (LOC100692532), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003446607.4

ORF Insert Sequence:

ATGCCTGATC ATGACAGCAC CACCCTCCTA ACAAGACAGA CCAAGCGCCG ACGTGTTGAC 
ATCGGGGTGA AAAGGACTGT GGGTAGCGCG ATATTCGCCC GCGCCAGGGA CACCCTGCTC 
GACAGAATGA ACCAATCCCA CGGCCCCGAT CAGGACGGCG ACTGCTCGCT GGTTGGTCAC 
AGCCACGGGG CAACCAACGG TGACGGCGAG AAGTCGAACG TTCTGAGAAA GCTGCTGAAG 
AGGGCGAACT CGTACGAGGA CGCCATAATG CCTTTCCCTG GGGCTACGAT CATCTCTCAG 
CTCTTGAAAA ACAACATGGG GAAGAACGGA GGCAGTGACT CGGGTTTTCA GGGGAGTGGT 
GCTCTGTCCA GTGGAGGCTC AGAGATCCAG GCCGAGGATG CCTGCAGCAA CTCCTCCCGT 
GATCGGGACA GCCCGTCTGA CTGCCTCTCT CCTGGGCCTC CTCTGCCCCC TCCATCTTCC 
TCCTCCTCCT TCGGGCGGCC ACCCCCTCCT ACGAGTCTCA ACTCCCACGC TCCCTCTCAG 
CCCCTCTCCC TTTCCTCCTT CGAATTGGAC CGGCTGTCCG ACGAGCACCT TCGTGCCAAG 
CGCGCCCGTG TGGAAAACAT CATCCGCGGC ATGAGCCATT CTCCGCTGGT CCGACCCAAT 
GCCGGCGACC ACAACCAAGA GAGCAACCGC GAAAATGAGA ACAGCAGCAA CAACAGCAAC 
AGCAGTAACG ACAACAGAGG CGACGGCAAC GGCCACAGCA CCGGCAGTCA CAGCCGGAGC 
GACTGCCCCT CTTCTCTGCT GAGCTCCCCT GGCTCGCGGG GTGGCATGGT TGGAGTCGGT 
GAGGTCTACA GGGAGAATAA GAGAAAGCAG CGCCTGCCAC AGCAGCAGCA CAGCTTCACC 
CAGCTGGTGT GTTCCAGGCA GGAGCAGAGA CAGGAAGAAA GAAGGCAGCT CAAACTACAG 
CTGGAAGACA TGCAGAAACA GCTCCGCCAG CTTCAGGAGA AGTTCTACCA GATCTACGAC 
TCGGAGACTG AGGAGGAGGA AGACAATGGC GAGAACGGTG AGCCTGACCG AGGCGGAGAA 
AGAGAGGAGG ACGGCAACTT GTCAGAGGAC AGCATCCGCT CCGACGGCCT GGAGGAGCGG 
CACAGAGAGA GGGGCCGGCG CGGCCACGAC GACCTCTCCG AGCTGGACCC GGGTCTGTTC 
CTGGACCGGG CACGGGCCCT CCTCCGAGAA CAGGCCTTGA TAGATGGAGA AATGGAGGAG 
GATGTGGACA GCAGGGAGGA CGAGGAGAGG AACGGGGAGA GGGTAATGAA GAGGAGGGGG 
CGGTGGGGCA GGGCCGGAGG AGGAGGAGGA GGAGGGAGGC AGTTAGCCGA GACTCTGAAG 
CAGGAGCTGA ACTGTGCCGT GTCACAGGTT GTCGACACCG TTGTCAAAGG CTTCTCCTCA 
CCCAAACAGG CCATTAATCA CCATCATGGC TGCCACAGCA GCACGCCGGC CGCGCCTTCC 
TCTTCCTCCA ACTCGTCCTC CTCGTGCCCT CCGCCTTTTG GCCCCATGCC CTCCCTCACC 
CCTGACTCTC GATTTGGTGG CGGCGCTTTG GCCCTCAGTC TGAACGGCGA TGGCAGTCCC 
ACTCCTAACT ACCACTCTTC CAACCAGAGG CTGCACTGCT TCGGGGACGT GATTGTCCCG 
AGCCCGCTGG ATTCGTTCGG CGGGTTGAGT GGCAGACTAA GTGGTGTGCC AACGCCCAAC 
GACCAAACAG AAGCACTGCC GCTGGTGGTG CGCAAGAGCG GTGGAGGAGG AGGAGGAGAG 
AACGCCGACC CCTCTCTTCC CCCTCCACCT CCTCCTCACC ATCCCTCTCT CCACCCGACT 
CCCCTCACAG CTTCGCTCGG GTTTAGCCCT CCATCCTTCC GCCACCCGTT CCCTCTTCCC 
CTCATGGGCT ATCCCTTTCA GAGCCCCCTG GGGTCACATG GGGGTGGAGC CGGGTACCCT 
CCGGGGCCCA AGGACCACCA CCGGTCCTCT CCCGATTCGA TGGACATAAC TCGGGAAACC 
AGCCTCAGAA CCAAAATGGC GTCCCTGGCG GGAGGCCATC TTGGTCACCA TCACCACAGG 
CAGAGCTCCC CAAACCAACA CGAGGGTCTG TCGCTATCCC TCATCAAGTC TGAGTGCGGG 
GACCTGCAAG ACATGTCTGA CATCTCGCCC TACTCTGGCA GCACCATTCA AGAAGGACTG 
TCTCCGAACC ACCTGAAGAA GGCCAAGCTG ATGTTCTTCT ACACCCGCTA TCCCTCATCC 
AACATGCTCA AAATGTACTT CTCTGATGTC AAGTTCAATC GATGCATCAC CTCCCAGCTG 
ATCAAGTGGT TCAGCAACTT CAGGGAGTTC TACTACATCC AGATGGAGAA GTTTGCTCGG 
CAGGCCATCA ACGAGGGCGT GACCGCTGCC GAGGAGCTGA CCGTGGGCCG AGATGCCGAA 
CTGTTCAGGG CACTCAACAT GCACTACAAC AAGGCCAACG ACTTTGAGGT TCCTGATCGA 
TTCCTGGAGG TGGCCCAGGT GACCCTTCGT GAGTTCTTCA ATGCCATCGT GGCTGGAAAG 
GATGCGGACC CATCCTGGAA GAAGGCCATC TACAAAGTGA TCTGCAAACT GGACAGCGAG 
GTTCCCGAGG TCTTCAAATC CCCCAACTGT CTACAAGAGC TCCTCCATGA CTGA