med22 cDNA ORF clone, Oncorhynchus mykiss(rainbow trout)

Gene
Clones
gRNAs

Summary

Gene Symbol	med22
Entrez Gene ID	110523314
Full Name	mediator complex subunit 22
Gene Type	protein-coding
Organism	Oncorhynchus mykiss(rainbow trout)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021601907.1	XP_021457582.1	mediator of RNA polymerase II transcription subunit 22 isoform X1
XM_021601908.1	XP_021457583.1	mediator of RNA polymerase II transcription subunit 22 isoform X2

Pathways from BioSystems
Gene Ontology

The following med22 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the med22 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OOf44041	XM_021601907.1 Latest version!	Oncorhynchus mykiss mediator complex subunit 22 (med22), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00
OOf44042	XM_021601908.1 Latest version!	Oncorhynchus mykiss mediator complex subunit 22 (med22), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$104.30 $149.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

med22 ( XM_021601907.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> XP_021457582.1 Oncorhynchus mykiss mediator complex subunit 22 (med22), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OOf44041
Clone ID Related Accession (Same CDS sequence)	XM_021601907.1
Accession Version	XM_021601907.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 615bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-26
Organism	Oncorhynchus mykiss(rainbow trout)
Product	mediator of RNA polymerase II transcription subunit 22 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035081.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oncorhynchus mykiss Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAATC AAAGAGCGCT CCCTCAAAGC AAGGAGAGTC TTCTTCAGAA CTACAACAAA 
AGACTGAAGG ATGATATCAG GTCCATTCTA GATAACCTCA CGGAAATCAT AAAAACCGCA 
AAGGTAGAGG ACGAGACACA GGTCTCTCGG GCTACTCAGG CTGAGCAGGA CCACTATGAG 
ATGCACGTCA GAGCAGCCAA CATTGTGCGT GCGGGCGAGT CCCTGATGAA GCTAGTGTCG 
GACCTGAAAC AGTTCCTGAT CCTCAACGAC TTCCCTTCGG TCAACGAGGC CATCAGCCTG 
CAGAACCAGC AGCTGCGCTC GCTGCAGGAG GAGTGCGACA AGAAGCTGAC CTCGCTCCGT 
GACGAGATCG CCATCGACCT GTACGAGCTA GAGGAAGAAT ATTACTCCTC CAGCTACGGT 
CAGTGGGACA GTACCGACCT GCCCCTGTGT GAGGCCTACT GGCGTTGGGA CAGTTGGGCG 
TCCCCGGACG GGGGCGCCAC CTCCGGGTCA GCCCAGGGGG TCGAGGAGGA CACAGACGGA 
CCTGCCTCCC AGGAGACCAC CCCCAAACAC CACCTCAACG GACACGGGAC CACCGCCGCA 
GCAGACAAGC CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021457582.1
CDS	122..736
Translation	MANQRALPQSKESLLQNYNKRLKDDIRSILDNLTEIIKTAKVEDETQVSRATQAEQDHYEMHVRAANIVRAGESLMKLVSDLKQFLILNDFPSVNEAISLQNQQLRSLQEECDKKLTSLRDEIAIDLYELEEEYYSSSYGQWDSTDLPLCEAYWRWDSWASPDGGATSGSAQGVEEDTDGPASQETTPKHHLNGHGTTAAADKP

Target ORF information:

RefSeq Version	XM_021601907.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss mediator complex subunit 22 (med22), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021601907.1

ORF Insert Sequence:

ATGGCCAATC AAAGAGCGCT CCCTCAAAGC AAGGAGAGTC TTCTTCAGAA CTACAACAAA 
AGACTGAAGG ATGATATCAG GTCCATTCTA GATAACCTCA CGGAAATCAT AAAAACCGCA 
AAGGTAGAGG ACGAGACACA GGTCTCTCGG GCTACTCAGG CTGAGCAGGA CCACTATGAG 
ATGCACGTCA GAGCAGCCAA CATTGTGCGT GCGGGCGAGT CCCTGATGAA GCTAGTGTCG 
GACCTGAAAC AGTTCCTGAT CCTCAACGAC TTCCCTTCGG TCAACGAGGC CATCAGCCTG 
CAGAACCAGC AGCTGCGCTC GCTGCAGGAG GAGTGCGACA AGAAGCTGAC CTCGCTCCGT 
GACGAGATCG CCATCGACCT GTACGAGCTA GAGGAAGAAT ATTACTCCTC CAGCTACGGT 
CAGTGGGACA GTACCGACCT GCCCCTGTGT GAGGCCTACT GGCGTTGGGA CAGTTGGGCG 
TCCCCGGACG GGGGCGCCAC CTCCGGGTCA GCCCAGGGGG TCGAGGAGGA CACAGACGGA 
CCTGCCTCCC AGGAGACCAC CCCCAAACAC CACCTCAACG GACACGGGAC CACCGCCGCA 
GCAGACAAGC CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

med22 ( XM_021601908.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> XP_021457583.1 Oncorhynchus mykiss mediator complex subunit 22 (med22), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OOf44042
Clone ID Related Accession (Same CDS sequence)	XM_021601908.1
Accession Version	XM_021601908.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 423bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-26
Organism	Oncorhynchus mykiss(rainbow trout)
Product	mediator of RNA polymerase II transcription subunit 22 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035081.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oncorhynchus mykiss Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAATC AAAGAGCGCT CCCTCAAAGC AAGGAGAGTC TTCTTCAGAA CTACAACAAA 
AGACTGAAGG ATGATATCAG GTCCATTCTA GATAACCTCA CGGAAATCAT AAAAACCGCA 
AAGGTAGAGG ACGAGACACA GGTCTCTCGG GCTACTCAGG CTGAGCAGGA CCACTATGAG 
ATGCACGTCA GAGCAGCCAA CATTGTGCGT GCGGGCGAGT CCCTGATGAA GCTAGTGTCG 
GACCTGAAAC AGTTCCTGAT CCTCAACGAC TTCCCTTCGG TCAACGAGGC CATCAGCCTG 
CAGAACCAGC AGCTGCGCTC GCTGCAGGAG GAGTGCGACA AGAAGCTGAC CTCGCTCCGT 
GACGAGATCG CCATCGACCT GTACGAGCTA GAGGAAGAAT ATTACTCCTC CAGGTACAAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021457583.1
CDS	123..545
Translation	MANQRALPQSKESLLQNYNKRLKDDIRSILDNLTEIIKTAKVEDETQVSRATQAEQDHYEMHVRAANIVRAGESLMKLVSDLKQFLILNDFPSVNEAISLQNQQLRSLQEECDKKLTSLRDEIAIDLYELEEEYYSSRYK

Target ORF information:

RefSeq Version	XM_021601908.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss mediator complex subunit 22 (med22), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021601908.1

ORF Insert Sequence:

ATGGCCAATC AAAGAGCGCT CCCTCAAAGC AAGGAGAGTC TTCTTCAGAA CTACAACAAA 
AGACTGAAGG ATGATATCAG GTCCATTCTA GATAACCTCA CGGAAATCAT AAAAACCGCA 
AAGGTAGAGG ACGAGACACA GGTCTCTCGG GCTACTCAGG CTGAGCAGGA CCACTATGAG 
ATGCACGTCA GAGCAGCCAA CATTGTGCGT GCGGGCGAGT CCCTGATGAA GCTAGTGTCG 
GACCTGAAAC AGTTCCTGAT CCTCAACGAC TTCCCTTCGG TCAACGAGGC CATCAGCCTG 
CAGAACCAGC AGCTGCGCTC GCTGCAGGAG GAGTGCGACA AGAAGCTGAC CTCGCTCCGT 
GACGAGATCG CCATCGACCT GTACGAGCTA GAGGAAGAAT ATTACTCCTC CAGGTACAAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.