ppat cDNA ORF clone, Oncorhynchus mykiss(rainbow trout)

Gene
Clones
gRNAs

Summary

Gene Symbol	ppat
Entrez Gene ID	110521685
Full Name	phosphoribosyl pyrophosphate amidotransferase
Gene Type	protein-coding
Organism	Oncorhynchus mykiss(rainbow trout)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021599445.1	XP_021455120.1	amidophosphoribosyltransferase isoform X1
XM_021599446.1	XP_021455121.1	amidophosphoribosyltransferase isoform X2

Pathways from BioSystems
Gene Ontology

The following ppat gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ppat cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OOf42114	XM_021599446.1 Latest version!	Oncorhynchus mykiss phosphoribosyl pyrophosphate amidotransferase (ppat), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
OOf42113	XM_021599445.1 Latest version!	Oncorhynchus mykiss phosphoribosyl pyrophosphate amidotransferase (ppat), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ppat ( XM_021599446.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> XP_021455121.1 Oncorhynchus mykiss phosphoribosyl pyrophosphate amidotransferase (ppat), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OOf42114
Clone ID Related Accession (Same CDS sequence)	XM_021599446.1
Accession Version	XM_021599446.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1329bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-26
Organism	Oncorhynchus mykiss(rainbow trout)
Product	amidophosphoribosyltransferase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035080.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oncorhynchus mykiss Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTTAG TGAACAACGC CTTCCCACCT GAGAACCTTC TGAAGCTGCG CTACGGTAAC 
CTGGGCATCG GGCACACACG CTACTCCACC ACGGGCATCT CTGAGCTGCA GAACTGCCAG 
CCCTTTGTGG TGGACACACT GCACGGCAAG ATCGCTGTGG CACACAACGG GGAGCTGGTC 
AATGCAGCCG CACTGCGTAA GAAGGTGATG CGTCACGGCG TGGGCCTGTC CACCAGCTCA 
GACAGTGAGC TCATCACCCA GCTGCTGGCT CTGACTCCAC CCATGGAGGA GCTGGATGCC 
CCTGACTGGG TTGCCAGGAT AAAGAACCTG ATGCATGAGA CCCCGACATC CTACTCGCTG 
CTGGTGATGT ACACAGATGT GATCTATGCT ATCCGTGACC CGTATGGAAA CAGACCCCTC 
AGTATTGGGC GCCTCGTCCC CATTTCTAAA CTCCACACTG CAGGTGCTGG CGAGGGGGAG 
ACAGAGGGCT GGGTGGTGTC ATCAGAGTCC TGCAGCTTCC AGTCCATCGG TGCCAAGTAC 
TACAGAGAGG TGATGCCTGG AGAGATAGTC CAGATCTCCA AACACGGTGT GAAGTCCCTA 
AGCATCGTCC CTCGCCCTGA GGGAGACCTC CCTGCATTCT GCATCTTTGA ATATGTCTAC 
TTTGCCAGAC CAGATTCCAT ATTTGAAGGT CAGATGGTGT ACACGGTGAG GCAGCGCTGT 
GGACGTCAGC TGGCCGTTGA GGCCCCTACA GAGGCAGACG TGGTCAGCAC TGTGCCCGAG 
TCTGCTACAC CGGCTGCACT GGGATATGCT CAACAGTCGG GACTGCCGTA CGTGGAGGTT 
CTGTGTAAGA ATCGCTATGT TGGGAGAACG TTTATTCAAC CAAACACACG CCTCAGGCAG 
TTGGGGGTGG CCAAGAAGTT TGGGGCACTG ACAGACAACT TTATAGGCAA GCGAGTGGTG 
CTCGTTGATG ACTCCATTGT CAGGGGCAAC ACCATCTCCC CCATAATAAA GCTCTTAAAG 
GAAGCAGGAG CCACAGAGGT TCACATCCGT GTTGCCTCAC CACCCATCAG GTTCCCATGC 
TACATGGGCA TCAACATCCC CACAAAAGAG GAACTCATCG CTAACAGGCC AGAGTTTGAG 
GACATTGCGG GCTACATTGG TGCAACCAGT GTGAGGTATC TGTCGGTTGA GGGCCTGTTA 
TCAGCGGTGC AGGGAGGAAT CCCCTCCCTC CAGGGGAAGG ATGAGAGGAT CAACACCAAT 
GCCAGCAGGA GAGTGGGCCA CTGCACCGCC TGCCTCACAG GCAAATACCC TGTGGAACTG 
GAGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021455121.1
CDS	90..1418
Translation	MGLVNNAFPPENLLKLRYGNLGIGHTRYSTTGISELQNCQPFVVDTLHGKIAVAHNGELVNAAALRKKVMRHGVGLSTSSDSELITQLLALTPPMEELDAPDWVARIKNLMHETPTSYSLLVMYTDVIYAIRDPYGNRPLSIGRLVPISKLHTAGAGEGETEGWVVSSESCSFQSIGAKYYREVMPGEIVQISKHGVKSLSIVPRPEGDLPAFCIFEYVYFARPDSIFEGQMVYTVRQRCGRQLAVEAPTEADVVSTVPESATPAALGYAQQSGLPYVEVLCKNRYVGRTFIQPNTRLRQLGVAKKFGALTDNFIGKRVVLVDDSIVRGNTISPIIKLLKEAGATEVHIRVASPPIRFPCYMGINIPTKEELIANRPEFEDIAGYIGATSVRYLSVEGLLSAVQGGIPSLQGKDERINTNASRRVGHCTACLTGKYPVELEW

Target ORF information:

RefSeq Version	XM_021599446.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss phosphoribosyl pyrophosphate amidotransferase (ppat), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021599446.1

ORF Insert Sequence:

ATGGGCTTAG TGAACAACGC CTTCCCACCT GAGAACCTTC TGAAGCTGCG CTACGGTAAC 
CTGGGCATCG GGCACACACG CTACTCCACC ACGGGCATCT CTGAGCTGCA GAACTGCCAG 
CCCTTTGTGG TGGACACACT GCACGGCAAG ATCGCTGTGG CACACAACGG GGAGCTGGTC 
AATGCAGCCG CACTGCGTAA GAAGGTGATG CGTCACGGCG TGGGCCTGTC CACCAGCTCA 
GACAGTGAGC TCATCACCCA GCTGCTGGCT CTGACTCCAC CCATGGAGGA GCTGGATGCC 
CCTGACTGGG TTGCCAGGAT AAAGAACCTG ATGCATGAGA CCCCGACATC CTACTCGCTG 
CTGGTGATGT ACACAGATGT GATCTATGCT ATCCGTGACC CGTATGGAAA CAGACCCCTC 
AGTATTGGGC GCCTCGTCCC CATTTCTAAA CTCCACACTG CAGGTGCTGG CGAGGGGGAG 
ACAGAGGGCT GGGTGGTGTC ATCAGAGTCC TGCAGCTTCC AGTCCATCGG TGCCAAGTAC 
TACAGAGAGG TGATGCCTGG AGAGATAGTC CAGATCTCCA AACACGGTGT GAAGTCCCTA 
AGCATCGTCC CTCGCCCTGA GGGAGACCTC CCTGCATTCT GCATCTTTGA ATATGTCTAC 
TTTGCCAGAC CAGATTCCAT ATTTGAAGGT CAGATGGTGT ACACGGTGAG GCAGCGCTGT 
GGACGTCAGC TGGCCGTTGA GGCCCCTACA GAGGCAGACG TGGTCAGCAC TGTGCCCGAG 
TCTGCTACAC CGGCTGCACT GGGATATGCT CAACAGTCGG GACTGCCGTA CGTGGAGGTT 
CTGTGTAAGA ATCGCTATGT TGGGAGAACG TTTATTCAAC CAAACACACG CCTCAGGCAG 
TTGGGGGTGG CCAAGAAGTT TGGGGCACTG ACAGACAACT TTATAGGCAA GCGAGTGGTG 
CTCGTTGATG ACTCCATTGT CAGGGGCAAC ACCATCTCCC CCATAATAAA GCTCTTAAAG 
GAAGCAGGAG CCACAGAGGT TCACATCCGT GTTGCCTCAC CACCCATCAG GTTCCCATGC 
TACATGGGCA TCAACATCCC CACAAAAGAG GAACTCATCG CTAACAGGCC AGAGTTTGAG 
GACATTGCGG GCTACATTGG TGCAACCAGT GTGAGGTATC TGTCGGTTGA GGGCCTGTTA 
TCAGCGGTGC AGGGAGGAAT CCCCTCCCTC CAGGGGAAGG ATGAGAGGAT CAACACCAAT 
GCCAGCAGGA GAGTGGGCCA CTGCACCGCC TGCCTCACAG GCAAATACCC TGTGGAACTG 
GAGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ppat ( XM_021599445.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> XP_021455120.1 Oncorhynchus mykiss phosphoribosyl pyrophosphate amidotransferase (ppat), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OOf42113
Clone ID Related Accession (Same CDS sequence)	XM_021599445.1
Accession Version	XM_021599445.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1530bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-26
Organism	Oncorhynchus mykiss(rainbow trout)
Product	amidophosphoribosyltransferase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035080.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oncorhynchus mykiss Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGAGT TCGAAGAATC TGGCATCGGA GAAGAGTGCG GAGTGTTTGG CTGTGTTGCA 
GCAGGGGAAT GGCCAACACA ACTAGAAGTT GCCAAAGTGT TAACTTTGGG TCTTGTAGCG 
CTACAGCACA GGGGTCAGGA AAGTGCTGGG ATTGTCACAA GCAATGGAGT CAACCCACCA 
ACGTACAACA CCCTCAAGGG AATGGGCTTA GTGAACAACG CCTTCCCACC TGAGAACCTT 
CTGAAGCTGC GCTACGGTAA CCTGGGCATC GGGCACACAC GCTACTCCAC CACGGGCATC 
TCTGAGCTGC AGAACTGCCA GCCCTTTGTG GTGGACACAC TGCACGGCAA GATCGCTGTG 
GCACACAACG GGGAGCTGGT CAATGCAGCC GCACTGCGTA AGAAGGTGAT GCGTCACGGC 
GTGGGCCTGT CCACCAGCTC AGACAGTGAG CTCATCACCC AGCTGCTGGC TCTGACTCCA 
CCCATGGAGG AGCTGGATGC CCCTGACTGG GTTGCCAGGA TAAAGAACCT GATGCATGAG 
ACCCCGACAT CCTACTCGCT GCTGGTGATG TACACAGATG TGATCTATGC TATCCGTGAC 
CCGTATGGAA ACAGACCCCT CAGTATTGGG CGCCTCGTCC CCATTTCTAA ACTCCACACT 
GCAGGTGCTG GCGAGGGGGA GACAGAGGGC TGGGTGGTGT CATCAGAGTC CTGCAGCTTC 
CAGTCCATCG GTGCCAAGTA CTACAGAGAG GTGATGCCTG GAGAGATAGT CCAGATCTCC 
AAACACGGTG TGAAGTCCCT AAGCATCGTC CCTCGCCCTG AGGGAGACCT CCCTGCATTC 
TGCATCTTTG AATATGTCTA CTTTGCCAGA CCAGATTCCA TATTTGAAGG TCAGATGGTG 
TACACGGTGA GGCAGCGCTG TGGACGTCAG CTGGCCGTTG AGGCCCCTAC AGAGGCAGAC 
GTGGTCAGCA CTGTGCCCGA GTCTGCTACA CCGGCTGCAC TGGGATATGC TCAACAGTCG 
GGACTGCCGT ACGTGGAGGT TCTGTGTAAG AATCGCTATG TTGGGAGAAC GTTTATTCAA 
CCAAACACAC GCCTCAGGCA GTTGGGGGTG GCCAAGAAGT TTGGGGCACT GACAGACAAC 
TTTATAGGCA AGCGAGTGGT GCTCGTTGAT GACTCCATTG TCAGGGGCAA CACCATCTCC 
CCCATAATAA AGCTCTTAAA GGAAGCAGGA GCCACAGAGG TTCACATCCG TGTTGCCTCA 
CCACCCATCA GGTTCCCATG CTACATGGGC ATCAACATCC CCACAAAAGA GGAACTCATC 
GCTAACAGGC CAGAGTTTGA GGACATTGCG GGCTACATTG GTGCAACCAG TGTGAGGTAT 
CTGTCGGTTG AGGGCCTGTT ATCAGCGGTG CAGGGAGGAA TCCCCTCCCT CCAGGGGAAG 
GATGAGAGGA TCAACACCAA TGCCAGCAGG AGAGTGGGCC ACTGCACCGC CTGCCTCACA 
GGCAAATACC CTGTGGAACT GGAGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021455120.1
CDS	462..1991
Translation	MSEFEESGIGEECGVFGCVAAGEWPTQLEVAKVLTLGLVALQHRGQESAGIVTSNGVNPPTYNTLKGMGLVNNAFPPENLLKLRYGNLGIGHTRYSTTGISELQNCQPFVVDTLHGKIAVAHNGELVNAAALRKKVMRHGVGLSTSSDSELITQLLALTPPMEELDAPDWVARIKNLMHETPTSYSLLVMYTDVIYAIRDPYGNRPLSIGRLVPISKLHTAGAGEGETEGWVVSSESCSFQSIGAKYYREVMPGEIVQISKHGVKSLSIVPRPEGDLPAFCIFEYVYFARPDSIFEGQMVYTVRQRCGRQLAVEAPTEADVVSTVPESATPAALGYAQQSGLPYVEVLCKNRYVGRTFIQPNTRLRQLGVAKKFGALTDNFIGKRVVLVDDSIVRGNTISPIIKLLKEAGATEVHIRVASPPIRFPCYMGINIPTKEELIANRPEFEDIAGYIGATSVRYLSVEGLLSAVQGGIPSLQGKDERINTNASRRVGHCTACLTGKYPVELEW

Target ORF information:

RefSeq Version	XM_021599445.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss phosphoribosyl pyrophosphate amidotransferase (ppat), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021599445.1

ORF Insert Sequence:

ATGAGCGAGT TCGAAGAATC TGGCATCGGA GAAGAGTGCG GAGTGTTTGG CTGTGTTGCA 
GCAGGGGAAT GGCCAACACA ACTAGAAGTT GCCAAAGTGT TAACTTTGGG TCTTGTAGCG 
CTACAGCACA GGGGTCAGGA AAGTGCTGGG ATTGTCACAA GCAATGGAGT CAACCCACCA 
ACGTACAACA CCCTCAAGGG AATGGGCTTA GTGAACAACG CCTTCCCACC TGAGAACCTT 
CTGAAGCTGC GCTACGGTAA CCTGGGCATC GGGCACACAC GCTACTCCAC CACGGGCATC 
TCTGAGCTGC AGAACTGCCA GCCCTTTGTG GTGGACACAC TGCACGGCAA GATCGCTGTG 
GCACACAACG GGGAGCTGGT CAATGCAGCC GCACTGCGTA AGAAGGTGAT GCGTCACGGC 
GTGGGCCTGT CCACCAGCTC AGACAGTGAG CTCATCACCC AGCTGCTGGC TCTGACTCCA 
CCCATGGAGG AGCTGGATGC CCCTGACTGG GTTGCCAGGA TAAAGAACCT GATGCATGAG 
ACCCCGACAT CCTACTCGCT GCTGGTGATG TACACAGATG TGATCTATGC TATCCGTGAC 
CCGTATGGAA ACAGACCCCT CAGTATTGGG CGCCTCGTCC CCATTTCTAA ACTCCACACT 
GCAGGTGCTG GCGAGGGGGA GACAGAGGGC TGGGTGGTGT CATCAGAGTC CTGCAGCTTC 
CAGTCCATCG GTGCCAAGTA CTACAGAGAG GTGATGCCTG GAGAGATAGT CCAGATCTCC 
AAACACGGTG TGAAGTCCCT AAGCATCGTC CCTCGCCCTG AGGGAGACCT CCCTGCATTC 
TGCATCTTTG AATATGTCTA CTTTGCCAGA CCAGATTCCA TATTTGAAGG TCAGATGGTG 
TACACGGTGA GGCAGCGCTG TGGACGTCAG CTGGCCGTTG AGGCCCCTAC AGAGGCAGAC 
GTGGTCAGCA CTGTGCCCGA GTCTGCTACA CCGGCTGCAC TGGGATATGC TCAACAGTCG 
GGACTGCCGT ACGTGGAGGT TCTGTGTAAG AATCGCTATG TTGGGAGAAC GTTTATTCAA 
CCAAACACAC GCCTCAGGCA GTTGGGGGTG GCCAAGAAGT TTGGGGCACT GACAGACAAC 
TTTATAGGCA AGCGAGTGGT GCTCGTTGAT GACTCCATTG TCAGGGGCAA CACCATCTCC 
CCCATAATAA AGCTCTTAAA GGAAGCAGGA GCCACAGAGG TTCACATCCG TGTTGCCTCA 
CCACCCATCA GGTTCCCATG CTACATGGGC ATCAACATCC CCACAAAAGA GGAACTCATC 
GCTAACAGGC CAGAGTTTGA GGACATTGCG GGCTACATTG GTGCAACCAG TGTGAGGTAT 
CTGTCGGTTG AGGGCCTGTT ATCAGCGGTG CAGGGAGGAA TCCCCTCCCT CCAGGGGAAG 
GATGAGAGGA TCAACACCAA TGCCAGCAGG AGAGTGGGCC ACTGCACCGC CTGCCTCACA 
GGCAAATACC CTGTGGAACT GGAGTGGTGA