il-20 cDNA ORF clone, Oncorhynchus mykiss(rainbow trout)

Gene
Clones
gRNAs

Summary

Gene Symbol	il-20
Entrez Gene ID	100499621
Full Name	putative interleukin 20 precursor
Gene Type	protein-coding
Organism	Oncorhynchus mykiss(rainbow trout)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001195186.1	NP_001182115.1	putative interleukin 20 precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Cloning of a novel interleukin (IL)-20-like gene in rainbow trout Oncorhynchus mykiss gives an insight into the evolution of the IL-10 family.
Wang T, D?az-Rosales P, Martin SA, Secombes CJ
Developmental and comparative immunology34(2)158-67(2010 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following il-20 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the il-20 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OOf01093	NM_001195186.1 Latest version!	Oncorhynchus mykiss putative interleukin 20 precursor (il-20), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

il-20 ( NM_001195186.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> NP_001182115.1 Oncorhynchus mykiss putative interleukin 20 precursor (il-20), mRNA.

Price & Availability↑

CloneID	OOf01093
Clone ID Related Accession (Same CDS sequence)	NM_001195186.1
Accession Version	NM_001195186.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 546bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-28
Organism	Oncorhynchus mykiss(rainbow trout)
Product	putative interleukin 20 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from FN386779.1. ##Evidence-Data-START## Transcript exon combination :: FN386779.1 [ECO:0000332] ##Evidence-Data-END##

ATGAAGCTGC TGTTAAGCCC CACCATCCCC AGGCTCCTCT TCCTGTTAGC GTGTCTCAGT 
GGGTGTGGCT TGGGACATGG CATACACCTG GGAACCTGCT CTGTCACCGT ACACACTCAC 
GAACTACGCA AGCACTACAC AGAGATACGG AGCTCTGTGA TAGCAGCAGA CAGTCAGATG 
GGAGTGAGAC TGCTGAGGGG AGACGTGATG AGGAACATAC AGGAGGGGGA GTACTGCTGT 
TTCCTGCGTC TTTTGCTACG TTTTTATGTG GAGAGAGTGT TTGTTAGCCA CGGCATGTCT 
CAGCCACTGC ACCGACGCTC AACCAGCGCT CTCGCTAATA GTTTCCTCAC AATCAACAAG 
AACCTGAGGC AATGTCACTG CCACTGTGGA GAAGACACCA GGAGAAAAAT GGACTCCCTA 
CAGGCCCAGT TTGACAAGCT GGAGATCTAC CAGGCAGCAG TAAAGGCTAT AGGAGAGCTG 
GACTCTCTAC TGGACTGGCT GGAAGAACTC AAGCACAACT CACACACAAC TCACACAGAC 
AGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001182115.1
CDS	297..842
Translation	MKLLLSPTIPRLLFLLACLSGCGLGHGIHLGTCSVTVHTHELRKHYTEIRSSVIAADSQMGVRLLRGDVMRNIQEGEYCCFLRLLLRFYVERVFVSHGMSQPLHRRSTSALANSFLTINKNLRQCHCHCGEDTRRKMDSLQAQFDKLEIYQAAVKAIGELDSLLDWLEELKHNSHTTHTDR

Target ORF information:

RefSeq Version	NM_001195186.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss putative interleukin 20 precursor (il-20), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001195186.1

ORF Insert Sequence:

ATGAAGCTGC TGTTAAGCCC CACCATCCCC AGGCTCCTCT TCCTGTTAGC GTGTCTCAGT 
GGGTGTGGCT TGGGACATGG CATACACCTG GGAACCTGCT CTGTCACCGT ACACACTCAC 
GAACTACGCA AGCACTACAC AGAGATACGG AGCTCTGTGA TAGCAGCAGA CAGTCAGATG 
GGAGTGAGAC TGCTGAGGGG AGACGTGATG AGGAACATAC AGGAGGGGGA GTACTGCTGT 
TTCCTGCGTC TTTTGCTACG TTTTTATGTG GAGAGAGTGT TTGTTAGCCA CGGCATGTCT 
CAGCCACTGC ACCGACGCTC AACCAGCGCT CTCGCTAATA GTTTCCTCAC AATCAACAAG 
AACCTGAGGC AATGTCACTG CCACTGTGGA GAAGACACCA GGAGAAAAAT GGACTCCCTA 
CAGGCCCAGT TTGACAAGCT GGAGATCTAC CAGGCAGCAG TAAAGGCTAT AGGAGAGCTG 
GACTCTCTAC TGGACTGGCT GGAAGAACTC AAGCACAACT CACACACAAC TCACACAGAC 
AGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cloning of a novel interleukin (IL)-20-like gene in rainbow trout Oncorhynchus mykiss gives an insight into the evolution of the IL-10 family.
Developmental and comparative immunology34(2)158-67(2010 Feb)
Wang T,D?az-Rosales P,Martin SA,Secombes CJ