defb4 cDNA ORF clone, Oncorhynchus mykiss(rainbow trout)

Gene
Clones
gRNAs

Summary

Gene Symbol	defb4
Entrez Gene ID	100499592
Full Name	defensin beta 4
Gene Type	protein-coding
Organism	Oncorhynchus mykiss(rainbow trout)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001195169.1	NP_001182098.1	beta-defensin 4 precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The rainbow trout genome provides novel insights into evolution after whole-genome duplication in vertebrates.
Berthelot C, Brunet F, Chalopin D, Juanchich A, Bernard M, No?l B, Bento P, Da Silva C, Labadie K, Alberti A, Aury JM, Louis A, Dehais P, Bardou P, Montfort J, Klopp C, Cabau C, Gaspin C, Thorgaard GH, Boussaha M, Quillet E, Guyomard R, Galiana D, Bobe J, Volff JN, Gen?t C, Wincker P, Jaillon O, Roest Crollius H, Guiguen Y
Nature communications53657(2014 Apr)

Characterization of the rainbow trout transcriptome using Sanger and 454-pyrosequencing approaches.
Salem M, Rexroad CE 3rd, Wang J, Thorgaard GH, Yao J
BMC genomics11564(2010 Oct)

Characterization of three novel beta-defensin antimicrobial peptides in rainbow trout (Oncorhynchus mykiss).
Casadei E, Wang T, Zou J, Gonz?lez Vecino JL, Wadsworth S, Secombes CJ
Molecular immunology46(16)3358-66(2009 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following defb4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the defb4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OOf01013	NM_001195169.1 Latest version!	Oncorhynchus mykiss defensin beta 4 (defb4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

defb4 ( NM_001195169.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> NP_001182098.1 Oncorhynchus mykiss defensin beta 4 (defb4), mRNA.

Price & Availability↑

CloneID	OOf01013
Clone ID Related Accession (Same CDS sequence)	NM_001195169.1
Accession Version	NM_001195169.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 207bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-07-09
Organism	Oncorhynchus mykiss(rainbow trout)
Product	beta-defensin 4 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from FM212658.1. ##Evidence-Data-START## Transcript exon combination :: FM212658.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN06640882 [ECO:0000348] ##Evidence-Data-END##

ATGAAATATC ACTGCACGAT GCTCTTGGTG CTCCTCGCTT TCTTGGCCAT CGCATGTGAT 
GTAAATGAAG CAGCTGCATT TCCAATTCCA TGGGGATGCT CAAACTACAG CGGGGTCTGC 
CGTGCTGTCT GTCTGTCAGC AGAACTACCA TTTGGACCTT TTGGATGTGC TAAAGGATTT 
GTATGCTGTG TCGCCCACGT CTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001182098.1
CDS	43..249
Translation	MKYHCTMLLVLLAFLAIACDVNEAAAFPIPWGCSNYSGVCRAVCLSAELPFGPFGCAKGFVCCVAHVF

Target ORF information:

RefSeq Version	NM_001195169.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss defensin beta 4 (defb4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001195169.1

ORF Insert Sequence:

ATGAAATATC ACTGCACGAT GCTCTTGGTG CTCCTCGCTT TCTTGGCCAT CGCATGTGAT 
GTAAATGAAG CAGCTGCATT TCCAATTCCA TGGGGATGCT CAAACTACAG CGGGGTCTGC 
CGTGCTGTCT GTCTGTCAGC AGAACTACCA TTTGGACCTT TTGGATGTGC TAAAGGATTT 
GTATGCTGTG TCGCCCACGT CTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The rainbow trout genome provides novel insights into evolution after whole-genome duplication in vertebrates.
Nature communications53657(2014 Apr)
Berthelot C,Brunet F,Chalopin D,Juanchich A,Bernard M,No?l B,Bento P,Da Silva C,Labadie K,Alberti A,Aury JM,Louis A,Dehais P,Bardou P,Montfort J,Klopp C,Cabau C,Gaspin C,Thorgaard GH,Boussaha M,Quillet E,Guyomard R,Galiana D,Bobe J,Volff JN,Gen?t C,Wincker P,Jaillon O,Roest Crollius H,Guiguen Y

Characterization of the rainbow trout transcriptome using Sanger and 454-pyrosequencing approaches.
BMC genomics11564(2010 Oct)
Salem M,Rexroad CE 3rd,Wang J,Thorgaard GH,Yao J

Characterization of three novel beta-defensin antimicrobial peptides in rainbow trout (Oncorhynchus mykiss).
Molecular immunology46(16)3358-66(2009 Oct)
Casadei E,Wang T,Zou J,Gonz?lez Vecino JL,Wadsworth S,Secombes CJ