il2 cDNA ORF clone, Oncorhynchus mykiss(rainbow trout)

Gene
Clones
gRNAs

Summary

Gene Symbol	il2
Entrez Gene ID	100302642
Full Name	interleukin 2
Synonyms	il-2
Gene Type	protein-coding
Organism	Oncorhynchus mykiss(rainbow trout)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001164065.1	NP_001157537.1	interleukin 2 precursor
NM_001164065.2	NP_001157537.1	interleukin 2 precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Rainbow trout interleukin-2: cloning, expression and bioactivity analysis.
D?az-Rosales P, Bird S, Wang TH, Fujiki K, Davidson WS, Zou J, Secombes CJ
Fish & shellfish immunology27(3)414-22(2009 Sep)

Conservation of structural and functional features in a primordial CD80/86 molecule from rainbow trout (Oncorhynchus mykiss), a primitive teleost fish.
Zhang YA, Hikima J, Li J, LaPatra SE, Luo YP, Sunyer JO
Journal of immunology (Baltimore, Md. : 1950)183(1)83-96(2009 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

IL-2 had moderate protein homology (30.9% identity/48.3% similarity) with Fugu IL-2, the only IL-2 homologue identified in fish to date, with lower homology to avian (17.8% identity/23.2% similarity) and mammalian (34.2 identity/46.5% similarity) IL-2s
Title: Rainbow trout interleukin-2: cloning, expression and bioactivity analysis.

The following il2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the il2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OOf00908	NM_001164065.2 Latest version!	Oncorhynchus mykiss interleukin 2 (il2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
View Old Accession Versions >>
OOf00908	NM_001164065.1	Oncorhynchus mykiss interleukin 2 (il2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

il2 ( NM_001164065.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> NP_001157537.1 Oncorhynchus mykiss interleukin 2 (il2), mRNA.

Price & Availability↑

CloneID	OOf00908
Clone ID Related Accession (Same CDS sequence)	NM_001164065.1 , NM_001164065.2
Accession Version	NM_001164065.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 429bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2013-04-16
Organism	Oncorhynchus mykiss(Rainbow trout)
Product	interleukin 2 precursor
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from FJ571513.1. ##Evidence-Data-START## Transcript exon combination :: FJ571513.1 [ECO:0000332] ##Evidence-Data-END##

ATGGACCGTC GTTACAGGAT TTCCTTTTTG ACGCTTTTTC TCACCGGTTG TCTACAAGGA 
AACCCAATTC CCAGACTCCT AGCTGGAATC GATTATCTAG AAGAAAATAT TACATGTCCA 
GATTCAGTCT TCTATACACC AACTGATGTA GAGGATAGTT GCATTGTTGC AGCATTGGCC 
TGTTCCATTA AGGAACTGGA CACTGTGAAA GTAGAATGCC TCGATAAAGC GGTCCATCTG 
GAAAGTATGC AACACCACAT CAGCATGACT GCCACGGCCC TACAAAAGAC GATTGATAAG 
GAGAACAGCA CAACGGACAC TTCAGAATGC ATCTGTGAAG ACAAGCGGTT GGAAAAGTCT 
TTCAAGGACT TCATTCAGAA CATAAGACAT TTAACTCAAG CTCATGCTGC AAAGCGTCTA 
AGTTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001157537.1
CDS	68..496
Translation	MDRRYRISFLTLFLTGCLQGNPIPRLLAGIDYLEENITCPDSVFYTPTDVEDSCIVAALACSIKELDTVKVECLDKAVHLESMQHHISMTATALQKTIDKENSTTDTSECICEDKRLEKSFKDFIQNIRHLTQAHAAKRLSS

Target ORF information:

RefSeq Version	NM_001164065.1
Organism	Oncorhynchus mykiss(Rainbow trout)
Definition	Oncorhynchus mykiss interleukin 2 (il2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001164065.1

ORF Insert Sequence:

ATGGACCGTC GTTACAGGAT TTCCTTTTTG ACGCTTTTTC TCACCGGTTG TCTACAAGGA 
AACCCAATTC CCAGACTCCT AGCTGGAATC GATTATCTAG AAGAAAATAT TACATGTCCA 
GATTCAGTCT TCTATACACC AACTGATGTA GAGGATAGTT GCATTGTTGC AGCATTGGCC 
TGTTCCATTA AGGAACTGGA CACTGTGAAA GTAGAATGCC TCGATAAAGC GGTCCATCTG 
GAAAGTATGC AACACCACAT CAGCATGACT GCCACGGCCC TACAAAAGAC GATTGATAAG 
GAGAACAGCA CAACGGACAC TTCAGAATGC ATCTGTGAAG ACAAGCGGTT GGAAAAGTCT 
TTCAAGGACT TCATTCAGAA CATAAGACAT TTAACTCAAG CTCATGCTGC AAAGCGTCTA 
AGTTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

il2 ( NM_001164065.2 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> NP_001157537.1 Oncorhynchus mykiss interleukin 2 (il2), mRNA.

Price & Availability↑

CloneID	OOf00908
Clone ID Related Accession (Same CDS sequence)	NM_001164065.1 , NM_001164065.2
Accession Version	NM_001164065.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 429bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-07-05
Organism	Oncorhynchus mykiss(rainbow trout)
Product	interleukin 2 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from FJ571513.1. On Jun 29, 2017 this sequence version replaced NM_001164065.1. ##Evidence-Data-START## Transcript exon combination :: FJ571513.1, FJ571512.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00041048 [ECO:0000348] ##Evidence-Data-END##

ATGGACCGTC GTTACAGGAT TTCCTTTTTG ACGCTTTTTC TCACCGGTTG TCTACAAGGA 
AACCCAATTC CCAGACTCCT AGCTGGAATC GATTATCTAG AAGAAAATAT TACATGTCCA 
GATTCAGTCT TCTATACACC AACTGATGTA GAGGATAGTT GCATTGTTGC AGCATTGGCC 
TGTTCCATTA AGGAACTGGA CACTGTGAAA GTAGAATGCC TCGATAAAGC GGTCCATCTG 
GAAAGTATGC AACACCACAT CAGCATGACT GCCACGGCCC TACAAAAGAC GATTGATAAG 
GAGAACAGCA CAACGGACAC TTCAGAATGC ATCTGTGAAG ACAAGCGGTT GGAAAAGTCT 
TTCAAGGACT TCATTCAGAA CATAAGACAT TTAACTCAAG CTCATGCTGC AAAGCGTCTA 
AGTTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001157537.1
CDS	67..495
Translation	MDRRYRISFLTLFLTGCLQGNPIPRLLAGIDYLEENITCPDSVFYTPTDVEDSCIVAALACSIKELDTVKVECLDKAVHLESMQHHISMTATALQKTIDKENSTTDTSECICEDKRLEKSFKDFIQNIRHLTQAHAAKRLSS

Target ORF information:

RefSeq Version	NM_001164065.2
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss interleukin 2 (il2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001164065.2

ORF Insert Sequence:

ATGGACCGTC GTTACAGGAT TTCCTTTTTG ACGCTTTTTC TCACCGGTTG TCTACAAGGA 
AACCCAATTC CCAGACTCCT AGCTGGAATC GATTATCTAG AAGAAAATAT TACATGTCCA 
GATTCAGTCT TCTATACACC AACTGATGTA GAGGATAGTT GCATTGTTGC AGCATTGGCC 
TGTTCCATTA AGGAACTGGA CACTGTGAAA GTAGAATGCC TCGATAAAGC GGTCCATCTG 
GAAAGTATGC AACACCACAT CAGCATGACT GCCACGGCCC TACAAAAGAC GATTGATAAG 
GAGAACAGCA CAACGGACAC TTCAGAATGC ATCTGTGAAG ACAAGCGGTT GGAAAAGTCT 
TTCAAGGACT TCATTCAGAA CATAAGACAT TTAACTCAAG CTCATGCTGC AAAGCGTCTA 
AGTTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Rainbow trout interleukin-2: cloning, expression and bioactivity analysis.
Fish & shellfish immunology27(3)414-22(2009 Sep)
D?az-Rosales P,Bird S,Wang TH,Fujiki K,Davidson WS,Zou J,Secombes CJ

Conservation of structural and functional features in a primordial CD80/86 molecule from rainbow trout (Oncorhynchus mykiss), a primitive teleost fish.
Journal of immunology (Baltimore, Md. : 1950)183(1)83-96(2009 Jul)
Zhang YA,Hikima J,Li J,LaPatra SE,Luo YP,Sunyer JO