ifng cDNA ORF clone, Oncorhynchus mykiss(rainbow trout)

Gene
Clones
gRNAs

Summary

Gene Symbol	ifng
Entrez Gene ID	100136643
Full Name	interferon gamma
Synonyms	IFNgamma2
Gene Type	protein-coding
Organism	Oncorhynchus mykiss(rainbow trout)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001124620.1	NP_001118092.1	interferon, gamma precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

The rainbow trout (Oncorhynchus mykiss) interferon response in the ovary.
Chaves-Pozo E, Zou J, Secombes CJ, Cuesta A, Tafalla C
Molecular immunology47(9)1757-64(2010 May)

Characterization of the interferon genes in homozygous rainbow trout reveals two novel genes, alternate splicing and differential regulation of duplicated genes.
Purcell MK, Laing KJ, Woodson JC, Thorgaard GH, Hansen JD
Fish & shellfish immunology26(2)293-304(2009 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data demonstrated that while VHSV strongly up-regulates all the IFN genes, IPNV in vivo exposure either has no effect or even provokes strong suppression of IFN gene expression.
Title: The rainbow trout (Oncorhynchus mykiss) interferon response in the ovary.

The following ifng gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ifng cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OOf00447	NM_001124620.1 Latest version!	Oncorhynchus mykiss interferon gamma (ifng), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ifng ( NM_001124620.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> NP_001118092.1 Oncorhynchus mykiss interferon gamma (ifng), mRNA.

Price & Availability↑

CloneID	OOf00447
Clone ID Related Accession (Same CDS sequence)	NM_001124620.1
Accession Version	NM_001124620.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 543bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-08-05
Organism	Oncorhynchus mykiss(rainbow trout)
Product	interferon, gamma precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AJ616215.1. ##Evidence-Data-START## Transcript exon combination :: AJ616215.1 [ECO:0000332] ##Evidence-Data-END##

ATGGATGTGT TATCAAGGGC TGTGATGTGT TTCTGCTTGA TGGGCTGGAT GACTTTAGGA 
TGGAGTAATG CTGCTCAGTT CACATCAATT AACATGAAGA GAAACATAGA CAAACTGAAA 
GTCCACTATA AGATCTCCAA GGACCAGCTG TTCAACGGAA ACCCTGTTTT CCCCAAGGAC 
ACGTTTGAGG ACAGTGAGCA GAGGGTGTTG ATGAGTGTGG TTCTGGACGT GTACCTGAGT 
ATCTTCGGCC AGATGCTGAA CCAGACGGGG GACCAGGAAA TGATCGAGAG TCTGAAATAT 
GTCAAGGGGA AAATTCAGGA TCTCCAGAAA CACTATTTCC TGGGGAGGAT ACCTGAGCTG 
AGGACACACC TGCAGAACCT GTGGGCCATC GAGACCAGTG ACACCACAGT CCAGGGGAAG 
GCTCTGTCCG AGTTCATTAC CATCTACGAG AAAGCCTCCA AACTGGCCCT TAAGTTCCAT 
CTAAAGAAGG ACAACCGCAG GAAGAGACGG CAAGCCCAGA GGCTCAAATC ACACATCATG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001118092.1
CDS	61..603
Translation	MDVLSRAVMCFCLMGWMTLGWSNAAQFTSINMKRNIDKLKVHYKISKDQLFNGNPVFPKDTFEDSEQRVLMSVVLDVYLSIFGQMLNQTGDQEMIESLKYVKGKIQDLQKHYFLGRIPELRTHLQNLWAIETSDTTVQGKALSEFITIYEKASKLALKFHLKKDNRRKRRQAQRLKSHIM

Target ORF information:

RefSeq Version	NM_001124620.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss interferon gamma (ifng), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001124620.1

ORF Insert Sequence:

ATGGATGTGT TATCAAGGGC TGTGATGTGT TTCTGCTTGA TGGGCTGGAT GACTTTAGGA 
TGGAGTAATG CTGCTCAGTT CACATCAATT AACATGAAGA GAAACATAGA CAAACTGAAA 
GTCCACTATA AGATCTCCAA GGACCAGCTG TTCAACGGAA ACCCTGTTTT CCCCAAGGAC 
ACGTTTGAGG ACAGTGAGCA GAGGGTGTTG ATGAGTGTGG TTCTGGACGT GTACCTGAGT 
ATCTTCGGCC AGATGCTGAA CCAGACGGGG GACCAGGAAA TGATCGAGAG TCTGAAATAT 
GTCAAGGGGA AAATTCAGGA TCTCCAGAAA CACTATTTCC TGGGGAGGAT ACCTGAGCTG 
AGGACACACC TGCAGAACCT GTGGGCCATC GAGACCAGTG ACACCACAGT CCAGGGGAAG 
GCTCTGTCCG AGTTCATTAC CATCTACGAG AAAGCCTCCA AACTGGCCCT TAAGTTCCAT 
CTAAAGAAGG ACAACCGCAG GAAGAGACGG CAAGCCCAGA GGCTCAAATC ACACATCATG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

The rainbow trout (Oncorhynchus mykiss) interferon response in the ovary.
Molecular immunology47(9)1757-64(2010 May)
Chaves-Pozo E,Zou J,Secombes CJ,Cuesta A,Tafalla C

Characterization of the interferon genes in homozygous rainbow trout reveals two novel genes, alternate splicing and differential regulation of duplicated genes.
Fish & shellfish immunology26(2)293-304(2009 Feb)
Purcell MK,Laing KJ,Woodson JC,Thorgaard GH,Hansen JD