LOC100136024 cDNA ORF clone, Oncorhynchus mykiss(rainbow trout)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100136024
Entrez Gene ID	100136024
Full Name	interleukin-1-beta
Synonyms	il1b
Gene Type	protein-coding
Organism	Oncorhynchus mykiss(rainbow trout)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001124347.2	NP_001117819.1	interleukin-1 beta

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

The rainbow trout genome provides novel insights into evolution after whole-genome duplication in vertebrates.
Berthelot C, Brunet F, Chalopin D, Juanchich A, Bernard M, No?l B, Bento P, Da Silva C, Labadie K, Alberti A, Aury JM, Louis A, Dehais P, Bardou P, Montfort J, Klopp C, Cabau C, Gaspin C, Thorgaard GH, Boussaha M, Quillet E, Guyomard R, Galiana D, Bobe J, Volff JN, Gen?t C, Wincker P, Jaillon O, Roest Crollius H, Guiguen Y
Nature communications53657(2014 Apr)

3D modelling of three pro-inflammatory molecules in selected fish species.
Costantini S, Facchiano AM, Randelli E, Casani D, Scapigliati G, Buonocore F
Current pharmaceutical design16(38)4203-12(2010)

Sequencing and expression of the second allele of the interleukin-1beta1 gene in rainbow trout (Oncorhynchus mykiss): identification of a novel SINE in the third intron.
Wang T, Johnson N, Zou J, Bols N, Secombes CJ
Fish & shellfish immunology16(3)335-58(2004 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The predicted 3D structures of IL-1beta, TNF-alpha, and IL-8 from Oncorhynchus mykiss, Dicentrarchus labrax, and Chionodraco hamatus are reported and the differences between them and their mammalian counterparts investigated.
Title: 3D modelling of three pro-inflammatory molecules in selected fish species.

The following LOC100136024 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100136024 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OOf00138	NM_001124347.2 Latest version!	Oncorhynchus mykiss interleukin-1-beta (LOC100136024), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100136024 ( NM_001124347.2 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> NP_001117819.1 Oncorhynchus mykiss interleukin-1-beta (LOC100136024), mRNA.

Price & Availability↑

CloneID	OOf00138
Clone ID Related Accession (Same CDS sequence)	NM_001124347.2
Accession Version	NM_001124347.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 783bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-22
Organism	Oncorhynchus mykiss(rainbow trout)
Product	interleukin-1 beta
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AJ223954.1. On May 14, 2013 this sequence version replaced NM_001124347.1. ##Evidence-Data-START## Transcript exon combination :: AJ223954.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00041048, SAMD00041049 [ECO:0000350] ##Evidence-Data-END##

ATGGATTTTG AGTCAAACTA CAGTTTAATA AAGAACACCT CTGAAAGTGC AGCATGGAGC 
TCCAAGCTGC CTCAGGGTCT GGATCTGGAG GTATCCCATC ACCCCATCAC CATGCGTCAC 
ATTGCCAACC TCATCATCGC CATGGAGAGG TTAAAGGGTG GCGAGGGGGT TACCATGGGA 
ACCGAGTTCA AGGACAAGGA CCTGCTCAAC TTCTTGCTGG AGAGTGCTGT GGAAGAACAT 
ATAGTGTTGG AGTTGGAGTC GGCACCCCCA GCGAGCAGGA GGGCAGCAGG GTTCAGCAGT 
ACATCACAGT ATGAGTGTAG CGTCACTGAC TCTGAGAACA AGTGCTGGGT CCTGATGAAT 
GAGGCTATGG AGCTGCACGC CATGATGCTC CAGGGAGGCA GCAGCTACCA CAAAGTGCAT 
TTGAACCTGT CTTCGTACGT CACGCCCGTC CCCATTGAGA CTGAAGCCAG ACCTGTAGCC 
CTAGGCATAA AGGGATCCAA CCTCTACCTG TCCTGCTCCA AATCGGGAGG CAGGCCCACC 
CTGCACCTAG AGGAGGTTGC GGACAAGGAT CAGCTGAAGT CCATCAGCCA GCAGAGCGAC 
ATGGTGCGTT TCCTTTTCTA CAGACGGAAC ACCGGGGTTG ACATCAGTAC CCTGGAGTCT 
GCCAGTTTCA GGAACTGGTT CATCAGCACG GACATGCAGC AGGACTACAC CAAACCGGTA 
GACATGTGTC AGAAGGCAGC CCCCAACCGC CTTACCACCT TCACCATCCA GCGCCACAAC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001117819.1
CDS	98..880
Translation	MDFESNYSLIKNTSESAAWSSKLPQGLDLEVSHHPITMRHIANLIIAMERLKGGEGVTMGTEFKDKDLLNFLLESAVEEHIVLELESAPPASRRAAGFSSTSQYECSVTDSENKCWVLMNEAMELHAMMLQGGSSYHKVHLNLSSYVTPVPIETEARPVALGIKGSNLYLSCSKSGGRPTLHLEEVADKDQLKSISQQSDMVRFLFYRRNTGVDISTLESASFRNWFISTDMQQDYTKPVDMCQKAAPNRLTTFTIQRHN

Target ORF information:

RefSeq Version	NM_001124347.2
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss interleukin-1-beta (LOC100136024), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001124347.2

ORF Insert Sequence:

ATGGATTTTG AGTCAAACTA CAGTTTAATA AAGAACACCT CTGAAAGTGC AGCATGGAGC 
TCCAAGCTGC CTCAGGGTCT GGATCTGGAG GTATCCCATC ACCCCATCAC CATGCGTCAC 
ATTGCCAACC TCATCATCGC CATGGAGAGG TTAAAGGGTG GCGAGGGGGT TACCATGGGA 
ACCGAGTTCA AGGACAAGGA CCTGCTCAAC TTCTTGCTGG AGAGTGCTGT GGAAGAACAT 
ATAGTGTTGG AGTTGGAGTC GGCACCCCCA GCGAGCAGGA GGGCAGCAGG GTTCAGCAGT 
ACATCACAGT ATGAGTGTAG CGTCACTGAC TCTGAGAACA AGTGCTGGGT CCTGATGAAT 
GAGGCTATGG AGCTGCACGC CATGATGCTC CAGGGAGGCA GCAGCTACCA CAAAGTGCAT 
TTGAACCTGT CTTCGTACGT CACGCCCGTC CCCATTGAGA CTGAAGCCAG ACCTGTAGCC 
CTAGGCATAA AGGGATCCAA CCTCTACCTG TCCTGCTCCA AATCGGGAGG CAGGCCCACC 
CTGCACCTAG AGGAGGTTGC GGACAAGGAT CAGCTGAAGT CCATCAGCCA GCAGAGCGAC 
ATGGTGCGTT TCCTTTTCTA CAGACGGAAC ACCGGGGTTG ACATCAGTAC CCTGGAGTCT 
GCCAGTTTCA GGAACTGGTT CATCAGCACG GACATGCAGC AGGACTACAC CAAACCGGTA 
GACATGTGTC AGAAGGCAGC CCCCAACCGC CTTACCACCT TCACCATCCA GCGCCACAAC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

The rainbow trout genome provides novel insights into evolution after whole-genome duplication in vertebrates.
Nature communications53657(2014 Apr)
Berthelot C,Brunet F,Chalopin D,Juanchich A,Bernard M,No?l B,Bento P,Da Silva C,Labadie K,Alberti A,Aury JM,Louis A,Dehais P,Bardou P,Montfort J,Klopp C,Cabau C,Gaspin C,Thorgaard GH,Boussaha M,Quillet E,Guyomard R,Galiana D,Bobe J,Volff JN,Gen?t C,Wincker P,Jaillon O,Roest Crollius H,Guiguen Y

3D modelling of three pro-inflammatory molecules in selected fish species.
Current pharmaceutical design16(38)4203-12(2010)
Costantini S,Facchiano AM,Randelli E,Casani D,Scapigliati G,Buonocore F

Sequencing and expression of the second allele of the interleukin-1beta1 gene in rainbow trout (Oncorhynchus mykiss): identification of a novel SINE in the third intron.
Fish & shellfish immunology16(3)335-58(2004 Mar)
Wang T,Johnson N,Zou J,Bols N,Secombes CJ