LOC106879323 cDNA ORF clone, Octopus bimaculoides

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC106879323
Entrez Gene ID	106879323
Full Name	valine--tRNA ligase-like
Gene Type	protein-coding
Organism	Octopus bimaculoides

mRNA and Protein(s)

mRNA	Protein	Name
XM_014928830.1	XP_014784316.1	valine--tRNA ligase-like isoform X1
XM_014928831.1	XP_014784317.1	valine--tRNA ligase-like isoform X2

Pathways from BioSystems
Gene Ontology

The following LOC106879323 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC106879323 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OOc31574	XM_014928830.1 Latest version!	Octopus bimaculoides valine--tRNA ligase-like (LOC106879323), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OOc31575	XM_014928831.1 Latest version!	Octopus bimaculoides valine--tRNA ligase-like (LOC106879323), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC106879323 ( XM_014928830.1 ) cDNA ORF clone, Octopus bimaculoides -> XP_014784316.1 Octopus bimaculoides valine--tRNA ligase-like (LOC106879323), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OOc31574
Clone ID Related Accession (Same CDS sequence)	XM_014928830.1
Accession Version	XM_014928830.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3210bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Octopus bimaculoides
Product	valine--tRNA ligase-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014696531.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Octopus bimaculoides Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTAGAAA GTAGTTTCGA TCAAACTGTG ATGGCTGCCT CCATCCAACA TGCCATGCTG 
TTGTCCCATT CTCACCTAAC ACTCAACACT TGTAGACGGA CATTTAATAA GTTTACAAAT 
AATCCCTATC ATAGTTATTC TCGAGCTATC AGGAATTGCC GAAAACTCAT ACAAAACCGA 
GTGACTCGAC CAAGTCAAGC TAAAATTTCG AATGCTTTGA ATTTAAGGTC TTTCTCACAG 
GCCACTGATG ACTCAGATTC AAATAACTGT CCAAGAGGCT TAGTTCCATG GCAAAAAAAA 
ATAGAATTTA CTAATATCCT TCCTCCAGCC TACAACCCAG AATACGTTGA AGCCATATGG 
TACAAATGGT GGGAACAGAA AGGTTTTCAC CAACCACAGC CAATTCAGGA TGCTGTAAAG 
AAGAGAAGGT TTGTCATCTG TTTGCCTCCC CCTAATGTGA CTGGTTCACT CCATCTTGGT 
CATGCTTTGA CATGCTCCAT TCAAGATGCT ATTGTCCGTT GGTACCGAAT GCAAGGACAC 
CAGGTACTCT GGGTCCCAGG ACTGGACCAT GCTGGCATTG CAACTCAAGT GATCATTGAG 
AAATGGTTAT GGAAGAAGAA GGGCTGCACA AAACATGATA TTGGCAGAGA AAAATTTCTA 
GAAGAAGTAT GGAAATGGAA AATAGAAAAA GAAAACACGA TTTATGAACA AATGAAACGA 
CTAGGAGCCT CACTTGACTG GACGCGACAT TTTTTTACAA TGGATCAGAA TCTCTCCAAA 
GCTATTAACG AAGCTTTTAT TCGACTCCAT GACGAGGGCT TAATATACCG TAAAAAATGT 
TTAGTCAACT GGTCTTGTGT TCTCAAGTCA GCCATTTCTG ATGTTGAGAT AGAAATGTTG 
CAGTTGAAAG GGAAGACCAA ACTCGAAATT CCTGGTTATG CTAAGAAAGT ACAGTTTGGA 
ACTTTAACCT CATTTGCATA TCCTGTTGTA GGAACTGATG AAAAGATCAT TGTGGCCACC 
ACTCGTCCAG AGACAATATT TGGGGATGTG GCTGTGGCTG TACACCCTGA AGATTCTCGT 
TACCAACATC TTCATGGAGC CTTACTTCAG CATCCATTTT CAGATCGAAA GATTCCAGTC 
ATTGCTGATG AGTTTGTCGA CAAAGAGTTT GGAGAAGGTG CTGTCAAACT GACCCCTTGT 
CATGATCAAA CGGACTATGA AGTTTGTGAG CGGCATGGTT TACCAATTTC CTTCTCTATC 
ATTGATGACT CTGGTCTTAT GTGCAATGTT GGTGCTCCTT TTGAGGGAAT GAAGAGGTTT 
GATGCAAGAA AAGCTGTTGT AAAATCCTTA AAACAGAAAG ACTTATACCA TGGAACGATC 
AACCATTCCA TGATGCTTCC TGTCTGCAGT CGTTCCAAAG ATGTTATTGA ACCTCTTCTG 
ATGGACCAGT GGTTCCTCAA TTGTAAAACA ATGGCAACCA AAGCAACTCA GGCTGTCACC 
AACAACCTCC TCTGCTTCAT CCCTGATTAC TATGACAGTA TCTGGTTCCA CTGGCTGCAG 
AATATCAGGG ACTGGTGTAT TTCCAGACAA TTGTGGTGGG GACACCAAAT ACCTGCTTAT 
TTCGCTACCA CTACGCCGTC ACCGCAGCTA TCAACCTCGA GTTCCACTGG AATATGGGTT 
AGTGCCAGAA ACCAAGAGGA AGCTCTAGCT AAAGCCAGTG AGAAATTATC TGTTCCCAAA 
GAACAGATAA CTCTTAAACA AGATGAAGAT GTCTTGGATA CTTGGTTTTC ATCAGCATTG 
CTTCCATTTT CAGTATTTGG ATGGCCAGAA AAAACAATTG ACTATGAATC TTTCTACCCA 
ACCGACCTTC TTGAGACTGG CAGTGATATC TTGTTCTTCT GGGTGGCCAG AATGGTTATG 
CTGGGACAGA AGTTAACAGG ACAGTTACCT TTCGATAAGA TTTTATTGCA TGGAATTATC 
CGAGACTCCG AAGGTAGAAA GATGAGTAAA TCTCAAGGAA ATGTGATCGA TCCATTGAAT 
GTCATTTATG GTGCATCCTT AAAGGACCTT CAGAACCAAC TGGACAGTAG TGTTCTTGAC 
CAAAAGGAAA TTGAACATTG TAAAAAAGAG CAACACAAAA ACTTCCCACA TGGTATTCCA 
CAGTGTGGCG CTGATGCTCT TAGATTCACG CTTTGTTCTT ATAATTTCAA ATTGCAAACC 
ATCAACATTC AAATGGACCA TATCAAAAGT TCAAAATTCT TTTGTAATAA AATATGGCAA 
GCTTCAAGAT TCATTCACCT CAACCAAGAA GATTCTTTCA CTCCCAAGCG ACCGTTCGCA 
CTGTCTGGTC ATGAATCTGC TATTGATAAA TGGATCCTCA GTGTTCTCAC TGAAACAACA 
CACTACTGTG ACCCAAAATT CCGCAATTAT GATCTACAGT TAGTTACCAA TTCCTTACGC 
AAGTTCTGGA TTCAGAATTT CTGTGATATT TATTTGGAAT CTGTGAAACC AATTTTAAAG 
AATGGTTCAG AAGAAGAAAA AGAAACCGTG AATCAAGTGT TACATTTCTG CCTGGAAACT 
TACCTCAAAG CCATCAGTCC TTTCATGCCT TACCTCAGTG AGGAACTCTA CCAACAACTT 
ATGCAAAGAA GCGGCCATTT TGATGTCAGT GTTTGCAGCG CTGCTTACCC TAACCCCAAG 
GATTTTACCT GGCGAAATCC ATTCATTGAA GAAGACATGA ATATTGTACA AGATCTCTGC 
AGTCAGGCAT TGTTTTTGAG GAAAGAATTT AGCTTGACCA AAGCTAAAGG AAACGTTTTC 
ATCAGTTCTT CCAATGAAAT CCTGCTGGAT CGCCTCTCTC AGAATTACTC TGTACCAATA 
TCCACACTCT CAAGATCGGC TTCAGTAAAC TTCATCTATG GAATCTTAAA TATTCCTCAC 
GGCTGTGTAT CATCAAAAAC AGTAGCTGAC ACTTGCCAAG TGCATTTAAG TCTTCAGGGT 
TTAGTGGATC CTGTCATTGA GTTGAACAAA CTCACTAAAA AGGTGGAGAA AGTGTTGAAA 
GAACTGGAGA AAATTGCAGC TTCAATGAAC AGAGATCTGA GAAATAACGA ACTTGTTGAT 
GAAAAGAAGA TAAAAGCTGT TGAAAACCTC AACTCTGAAC TTGACCGGCT TCAGAACTAT 
GTGTCCGTCT TAGAAAAACT GGAACCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014784316.1
CDS	5..3214
Translation	MLESSFDQTVMAASIQHAMLLSHSHLTLNTCRRTFNKFTNNPYHSYSRAIRNCRKLIQNRVTRPSQAKISNALNLRSFSQATDDSDSNNCPRGLVPWQKKIEFTNILPPAYNPEYVEAIWYKWWEQKGFHQPQPIQDAVKKRRFVICLPPPNVTGSLHLGHALTCSIQDAIVRWYRMQGHQVLWVPGLDHAGIATQVIIEKWLWKKKGCTKHDIGREKFLEEVWKWKIEKENTIYEQMKRLGASLDWTRHFFTMDQNLSKAINEAFIRLHDEGLIYRKKCLVNWSCVLKSAISDVEIEMLQLKGKTKLEIPGYAKKVQFGTLTSFAYPVVGTDEKIIVATTRPETIFGDVAVAVHPEDSRYQHLHGALLQHPFSDRKIPVIADEFVDKEFGEGAVKLTPCHDQTDYEVCERHGLPISFSIIDDSGLMCNVGAPFEGMKRFDARKAVVKSLKQKDLYHGTINHSMMLPVCSRSKDVIEPLLMDQWFLNCKTMATKATQAVTNNLLCFIPDYYDSIWFHWLQNIRDWCISRQLWWGHQIPAYFATTTPSPQLSTSSSTGIWVSARNQEEALAKASEKLSVPKEQITLKQDEDVLDTWFSSALLPFSVFGWPEKTIDYESFYPTDLLETGSDILFFWVARMVMLGQKLTGQLPFDKILLHGIIRDSEGRKMSKSQGNVIDPLNVIYGASLKDLQNQLDSSVLDQKEIEHCKKEQHKNFPHGIPQCGADALRFTLCSYNFKLQTINIQMDHIKSSKFFCNKIWQASRFIHLNQEDSFTPKRPFALSGHESAIDKWILSVLTETTHYCDPKFRNYDLQLVTNSLRKFWIQNFCDIYLESVKPILKNGSEEEKETVNQVLHFCLETYLKAISPFMPYLSEELYQQLMQRSGHFDVSVCSAAYPNPKDFTWRNPFIEEDMNIVQDLCSQALFLRKEFSLTKAKGNVFISSSNEILLDRLSQNYSVPISTLSRSASVNFIYGILNIPHGCVSSKTVADTCQVHLSLQGLVDPVIELNKLTKKVEKVLKELEKIAASMNRDLRNNELVDEKKIKAVENLNSELDRLQNYVSVLEKLEP

Target ORF information:

RefSeq Version	XM_014928830.1
Organism	Octopus bimaculoides
Definition	Octopus bimaculoides valine--tRNA ligase-like (LOC106879323), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014928830.1

ORF Insert Sequence:

ATGTTAGAAA GTAGTTTCGA TCAAACTGTG ATGGCTGCCT CCATCCAACA TGCCATGCTG 
TTGTCCCATT CTCACCTAAC ACTCAACACT TGTAGACGGA CATTTAATAA GTTTACAAAT 
AATCCCTATC ATAGTTATTC TCGAGCTATC AGGAATTGCC GAAAACTCAT ACAAAACCGA 
GTGACTCGAC CAAGTCAAGC TAAAATTTCG AATGCTTTGA ATTTAAGGTC TTTCTCACAG 
GCCACTGATG ACTCAGATTC AAATAACTGT CCAAGAGGCT TAGTTCCATG GCAAAAAAAA 
ATAGAATTTA CTAATATCCT TCCTCCAGCC TACAACCCAG AATACGTTGA AGCCATATGG 
TACAAATGGT GGGAACAGAA AGGTTTTCAC CAACCACAGC CAATTCAGGA TGCTGTAAAG 
AAGAGAAGGT TTGTCATCTG TTTGCCTCCC CCTAATGTGA CTGGTTCACT CCATCTTGGT 
CATGCTTTGA CATGCTCCAT TCAAGATGCT ATTGTCCGTT GGTACCGAAT GCAAGGACAC 
CAGGTACTCT GGGTCCCAGG ACTGGACCAT GCTGGCATTG CAACTCAAGT GATCATTGAG 
AAATGGTTAT GGAAGAAGAA GGGCTGCACA AAACATGATA TTGGCAGAGA AAAATTTCTA 
GAAGAAGTAT GGAAATGGAA AATAGAAAAA GAAAACACGA TTTATGAACA AATGAAACGA 
CTAGGAGCCT CACTTGACTG GACGCGACAT TTTTTTACAA TGGATCAGAA TCTCTCCAAA 
GCTATTAACG AAGCTTTTAT TCGACTCCAT GACGAGGGCT TAATATACCG TAAAAAATGT 
TTAGTCAACT GGTCTTGTGT TCTCAAGTCA GCCATTTCTG ATGTTGAGAT AGAAATGTTG 
CAGTTGAAAG GGAAGACCAA ACTCGAAATT CCTGGTTATG CTAAGAAAGT ACAGTTTGGA 
ACTTTAACCT CATTTGCATA TCCTGTTGTA GGAACTGATG AAAAGATCAT TGTGGCCACC 
ACTCGTCCAG AGACAATATT TGGGGATGTG GCTGTGGCTG TACACCCTGA AGATTCTCGT 
TACCAACATC TTCATGGAGC CTTACTTCAG CATCCATTTT CAGATCGAAA GATTCCAGTC 
ATTGCTGATG AGTTTGTCGA CAAAGAGTTT GGAGAAGGTG CTGTCAAACT GACCCCTTGT 
CATGATCAAA CGGACTATGA AGTTTGTGAG CGGCATGGTT TACCAATTTC CTTCTCTATC 
ATTGATGACT CTGGTCTTAT GTGCAATGTT GGTGCTCCTT TTGAGGGAAT GAAGAGGTTT 
GATGCAAGAA AAGCTGTTGT AAAATCCTTA AAACAGAAAG ACTTATACCA TGGAACGATC 
AACCATTCCA TGATGCTTCC TGTCTGCAGT CGTTCCAAAG ATGTTATTGA ACCTCTTCTG 
ATGGACCAGT GGTTCCTCAA TTGTAAAACA ATGGCAACCA AAGCAACTCA GGCTGTCACC 
AACAACCTCC TCTGCTTCAT CCCTGATTAC TATGACAGTA TCTGGTTCCA CTGGCTGCAG 
AATATCAGGG ACTGGTGTAT TTCCAGACAA TTGTGGTGGG GACACCAAAT ACCTGCTTAT 
TTCGCTACCA CTACGCCGTC ACCGCAGCTA TCAACCTCGA GTTCCACTGG AATATGGGTT 
AGTGCCAGAA ACCAAGAGGA AGCTCTAGCT AAAGCCAGTG AGAAATTATC TGTTCCCAAA 
GAACAGATAA CTCTTAAACA AGATGAAGAT GTCTTGGATA CTTGGTTTTC ATCAGCATTG 
CTTCCATTTT CAGTATTTGG ATGGCCAGAA AAAACAATTG ACTATGAATC TTTCTACCCA 
ACCGACCTTC TTGAGACTGG CAGTGATATC TTGTTCTTCT GGGTGGCCAG AATGGTTATG 
CTGGGACAGA AGTTAACAGG ACAGTTACCT TTCGATAAGA TTTTATTGCA TGGAATTATC 
CGAGACTCCG AAGGTAGAAA GATGAGTAAA TCTCAAGGAA ATGTGATCGA TCCATTGAAT 
GTCATTTATG GTGCATCCTT AAAGGACCTT CAGAACCAAC TGGACAGTAG TGTTCTTGAC 
CAAAAGGAAA TTGAACATTG TAAAAAAGAG CAACACAAAA ACTTCCCACA TGGTATTCCA 
CAGTGTGGCG CTGATGCTCT TAGATTCACG CTTTGTTCTT ATAATTTCAA ATTGCAAACC 
ATCAACATTC AAATGGACCA TATCAAAAGT TCAAAATTCT TTTGTAATAA AATATGGCAA 
GCTTCAAGAT TCATTCACCT CAACCAAGAA GATTCTTTCA CTCCCAAGCG ACCGTTCGCA 
CTGTCTGGTC ATGAATCTGC TATTGATAAA TGGATCCTCA GTGTTCTCAC TGAAACAACA 
CACTACTGTG ACCCAAAATT CCGCAATTAT GATCTACAGT TAGTTACCAA TTCCTTACGC 
AAGTTCTGGA TTCAGAATTT CTGTGATATT TATTTGGAAT CTGTGAAACC AATTTTAAAG 
AATGGTTCAG AAGAAGAAAA AGAAACCGTG AATCAAGTGT TACATTTCTG CCTGGAAACT 
TACCTCAAAG CCATCAGTCC TTTCATGCCT TACCTCAGTG AGGAACTCTA CCAACAACTT 
ATGCAAAGAA GCGGCCATTT TGATGTCAGT GTTTGCAGCG CTGCTTACCC TAACCCCAAG 
GATTTTACCT GGCGAAATCC ATTCATTGAA GAAGACATGA ATATTGTACA AGATCTCTGC 
AGTCAGGCAT TGTTTTTGAG GAAAGAATTT AGCTTGACCA AAGCTAAAGG AAACGTTTTC 
ATCAGTTCTT CCAATGAAAT CCTGCTGGAT CGCCTCTCTC AGAATTACTC TGTACCAATA 
TCCACACTCT CAAGATCGGC TTCAGTAAAC TTCATCTATG GAATCTTAAA TATTCCTCAC 
GGCTGTGTAT CATCAAAAAC AGTAGCTGAC ACTTGCCAAG TGCATTTAAG TCTTCAGGGT 
TTAGTGGATC CTGTCATTGA GTTGAACAAA CTCACTAAAA AGGTGGAGAA AGTGTTGAAA 
GAACTGGAGA AAATTGCAGC TTCAATGAAC AGAGATCTGA GAAATAACGA ACTTGTTGAT 
GAAAAGAAGA TAAAAGCTGT TGAAAACCTC AACTCTGAAC TTGACCGGCT TCAGAACTAT 
GTGTCCGTCT TAGAAAAACT GGAACCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC106879323 ( XM_014928831.1 ) cDNA ORF clone, Octopus bimaculoides -> XP_014784317.1 Octopus bimaculoides valine--tRNA ligase-like (LOC106879323), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OOc31575
Clone ID Related Accession (Same CDS sequence)	XM_014928831.1
Accession Version	XM_014928831.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3207bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Octopus bimaculoides
Product	valine--tRNA ligase-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014696531.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Octopus bimaculoides Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTAGAAA GTAGTTTCGA TCAAACTGTG ATGGCTGCCT CCATCCAACA TGCCATGCTG 
TTGTCCCATT CTCACCTAAC ACTCAACACT TGTAGACGGA CATTTAATAA GTTTACAAAT 
AATCCCTATC ATAGTTATTC TCGAGCTATC AGGAATTGCC GAAAACTCAT ACAAAACCGA 
GTGACTCGAC CAAGTCAAGC TAAAATTTCG AATGCTTTGA ATTTAAGGTC TTTCTCACAG 
GCCACTGATG ACTCAGATTC AAATAACTGT CCAAGAGGCT TAGTTCCATG GCAAAAAAAA 
GAATTTACTA ATATCCTTCC TCCAGCCTAC AACCCAGAAT ACGTTGAAGC CATATGGTAC 
AAATGGTGGG AACAGAAAGG TTTTCACCAA CCACAGCCAA TTCAGGATGC TGTAAAGAAG 
AGAAGGTTTG TCATCTGTTT GCCTCCCCCT AATGTGACTG GTTCACTCCA TCTTGGTCAT 
GCTTTGACAT GCTCCATTCA AGATGCTATT GTCCGTTGGT ACCGAATGCA AGGACACCAG 
GTACTCTGGG TCCCAGGACT GGACCATGCT GGCATTGCAA CTCAAGTGAT CATTGAGAAA 
TGGTTATGGA AGAAGAAGGG CTGCACAAAA CATGATATTG GCAGAGAAAA ATTTCTAGAA 
GAAGTATGGA AATGGAAAAT AGAAAAAGAA AACACGATTT ATGAACAAAT GAAACGACTA 
GGAGCCTCAC TTGACTGGAC GCGACATTTT TTTACAATGG ATCAGAATCT CTCCAAAGCT 
ATTAACGAAG CTTTTATTCG ACTCCATGAC GAGGGCTTAA TATACCGTAA AAAATGTTTA 
GTCAACTGGT CTTGTGTTCT CAAGTCAGCC ATTTCTGATG TTGAGATAGA AATGTTGCAG 
TTGAAAGGGA AGACCAAACT CGAAATTCCT GGTTATGCTA AGAAAGTACA GTTTGGAACT 
TTAACCTCAT TTGCATATCC TGTTGTAGGA ACTGATGAAA AGATCATTGT GGCCACCACT 
CGTCCAGAGA CAATATTTGG GGATGTGGCT GTGGCTGTAC ACCCTGAAGA TTCTCGTTAC 
CAACATCTTC ATGGAGCCTT ACTTCAGCAT CCATTTTCAG ATCGAAAGAT TCCAGTCATT 
GCTGATGAGT TTGTCGACAA AGAGTTTGGA GAAGGTGCTG TCAAACTGAC CCCTTGTCAT 
GATCAAACGG ACTATGAAGT TTGTGAGCGG CATGGTTTAC CAATTTCCTT CTCTATCATT 
GATGACTCTG GTCTTATGTG CAATGTTGGT GCTCCTTTTG AGGGAATGAA GAGGTTTGAT 
GCAAGAAAAG CTGTTGTAAA ATCCTTAAAA CAGAAAGACT TATACCATGG AACGATCAAC 
CATTCCATGA TGCTTCCTGT CTGCAGTCGT TCCAAAGATG TTATTGAACC TCTTCTGATG 
GACCAGTGGT TCCTCAATTG TAAAACAATG GCAACCAAAG CAACTCAGGC TGTCACCAAC 
AACCTCCTCT GCTTCATCCC TGATTACTAT GACAGTATCT GGTTCCACTG GCTGCAGAAT 
ATCAGGGACT GGTGTATTTC CAGACAATTG TGGTGGGGAC ACCAAATACC TGCTTATTTC 
GCTACCACTA CGCCGTCACC GCAGCTATCA ACCTCGAGTT CCACTGGAAT ATGGGTTAGT 
GCCAGAAACC AAGAGGAAGC TCTAGCTAAA GCCAGTGAGA AATTATCTGT TCCCAAAGAA 
CAGATAACTC TTAAACAAGA TGAAGATGTC TTGGATACTT GGTTTTCATC AGCATTGCTT 
CCATTTTCAG TATTTGGATG GCCAGAAAAA ACAATTGACT ATGAATCTTT CTACCCAACC 
GACCTTCTTG AGACTGGCAG TGATATCTTG TTCTTCTGGG TGGCCAGAAT GGTTATGCTG 
GGACAGAAGT TAACAGGACA GTTACCTTTC GATAAGATTT TATTGCATGG AATTATCCGA 
GACTCCGAAG GTAGAAAGAT GAGTAAATCT CAAGGAAATG TGATCGATCC ATTGAATGTC 
ATTTATGGTG CATCCTTAAA GGACCTTCAG AACCAACTGG ACAGTAGTGT TCTTGACCAA 
AAGGAAATTG AACATTGTAA AAAAGAGCAA CACAAAAACT TCCCACATGG TATTCCACAG 
TGTGGCGCTG ATGCTCTTAG ATTCACGCTT TGTTCTTATA ATTTCAAATT GCAAACCATC 
AACATTCAAA TGGACCATAT CAAAAGTTCA AAATTCTTTT GTAATAAAAT ATGGCAAGCT 
TCAAGATTCA TTCACCTCAA CCAAGAAGAT TCTTTCACTC CCAAGCGACC GTTCGCACTG 
TCTGGTCATG AATCTGCTAT TGATAAATGG ATCCTCAGTG TTCTCACTGA AACAACACAC 
TACTGTGACC CAAAATTCCG CAATTATGAT CTACAGTTAG TTACCAATTC CTTACGCAAG 
TTCTGGATTC AGAATTTCTG TGATATTTAT TTGGAATCTG TGAAACCAAT TTTAAAGAAT 
GGTTCAGAAG AAGAAAAAGA AACCGTGAAT CAAGTGTTAC ATTTCTGCCT GGAAACTTAC 
CTCAAAGCCA TCAGTCCTTT CATGCCTTAC CTCAGTGAGG AACTCTACCA ACAACTTATG 
CAAAGAAGCG GCCATTTTGA TGTCAGTGTT TGCAGCGCTG CTTACCCTAA CCCCAAGGAT 
TTTACCTGGC GAAATCCATT CATTGAAGAA GACATGAATA TTGTACAAGA TCTCTGCAGT 
CAGGCATTGT TTTTGAGGAA AGAATTTAGC TTGACCAAAG CTAAAGGAAA CGTTTTCATC 
AGTTCTTCCA ATGAAATCCT GCTGGATCGC CTCTCTCAGA ATTACTCTGT ACCAATATCC 
ACACTCTCAA GATCGGCTTC AGTAAACTTC ATCTATGGAA TCTTAAATAT TCCTCACGGC 
TGTGTATCAT CAAAAACAGT AGCTGACACT TGCCAAGTGC ATTTAAGTCT TCAGGGTTTA 
GTGGATCCTG TCATTGAGTT GAACAAACTC ACTAAAAAGG TGGAGAAAGT GTTGAAAGAA 
CTGGAGAAAA TTGCAGCTTC AATGAACAGA GATCTGAGAA ATAACGAACT TGTTGATGAA 
AAGAAGATAA AAGCTGTTGA AAACCTCAAC TCTGAACTTG ACCGGCTTCA GAACTATGTG 
TCCGTCTTAG AAAAACTGGA ACCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014784317.1
CDS	5..3211
Translation	MLESSFDQTVMAASIQHAMLLSHSHLTLNTCRRTFNKFTNNPYHSYSRAIRNCRKLIQNRVTRPSQAKISNALNLRSFSQATDDSDSNNCPRGLVPWQKKEFTNILPPAYNPEYVEAIWYKWWEQKGFHQPQPIQDAVKKRRFVICLPPPNVTGSLHLGHALTCSIQDAIVRWYRMQGHQVLWVPGLDHAGIATQVIIEKWLWKKKGCTKHDIGREKFLEEVWKWKIEKENTIYEQMKRLGASLDWTRHFFTMDQNLSKAINEAFIRLHDEGLIYRKKCLVNWSCVLKSAISDVEIEMLQLKGKTKLEIPGYAKKVQFGTLTSFAYPVVGTDEKIIVATTRPETIFGDVAVAVHPEDSRYQHLHGALLQHPFSDRKIPVIADEFVDKEFGEGAVKLTPCHDQTDYEVCERHGLPISFSIIDDSGLMCNVGAPFEGMKRFDARKAVVKSLKQKDLYHGTINHSMMLPVCSRSKDVIEPLLMDQWFLNCKTMATKATQAVTNNLLCFIPDYYDSIWFHWLQNIRDWCISRQLWWGHQIPAYFATTTPSPQLSTSSSTGIWVSARNQEEALAKASEKLSVPKEQITLKQDEDVLDTWFSSALLPFSVFGWPEKTIDYESFYPTDLLETGSDILFFWVARMVMLGQKLTGQLPFDKILLHGIIRDSEGRKMSKSQGNVIDPLNVIYGASLKDLQNQLDSSVLDQKEIEHCKKEQHKNFPHGIPQCGADALRFTLCSYNFKLQTINIQMDHIKSSKFFCNKIWQASRFIHLNQEDSFTPKRPFALSGHESAIDKWILSVLTETTHYCDPKFRNYDLQLVTNSLRKFWIQNFCDIYLESVKPILKNGSEEEKETVNQVLHFCLETYLKAISPFMPYLSEELYQQLMQRSGHFDVSVCSAAYPNPKDFTWRNPFIEEDMNIVQDLCSQALFLRKEFSLTKAKGNVFISSSNEILLDRLSQNYSVPISTLSRSASVNFIYGILNIPHGCVSSKTVADTCQVHLSLQGLVDPVIELNKLTKKVEKVLKELEKIAASMNRDLRNNELVDEKKIKAVENLNSELDRLQNYVSVLEKLEP

Target ORF information:

RefSeq Version	XM_014928831.1
Organism	Octopus bimaculoides
Definition	Octopus bimaculoides valine--tRNA ligase-like (LOC106879323), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014928831.1

ORF Insert Sequence:

ATGTTAGAAA GTAGTTTCGA TCAAACTGTG ATGGCTGCCT CCATCCAACA TGCCATGCTG 
TTGTCCCATT CTCACCTAAC ACTCAACACT TGTAGACGGA CATTTAATAA GTTTACAAAT 
AATCCCTATC ATAGTTATTC TCGAGCTATC AGGAATTGCC GAAAACTCAT ACAAAACCGA 
GTGACTCGAC CAAGTCAAGC TAAAATTTCG AATGCTTTGA ATTTAAGGTC TTTCTCACAG 
GCCACTGATG ACTCAGATTC AAATAACTGT CCAAGAGGCT TAGTTCCATG GCAAAAAAAA 
GAATTTACTA ATATCCTTCC TCCAGCCTAC AACCCAGAAT ACGTTGAAGC CATATGGTAC 
AAATGGTGGG AACAGAAAGG TTTTCACCAA CCACAGCCAA TTCAGGATGC TGTAAAGAAG 
AGAAGGTTTG TCATCTGTTT GCCTCCCCCT AATGTGACTG GTTCACTCCA TCTTGGTCAT 
GCTTTGACAT GCTCCATTCA AGATGCTATT GTCCGTTGGT ACCGAATGCA AGGACACCAG 
GTACTCTGGG TCCCAGGACT GGACCATGCT GGCATTGCAA CTCAAGTGAT CATTGAGAAA 
TGGTTATGGA AGAAGAAGGG CTGCACAAAA CATGATATTG GCAGAGAAAA ATTTCTAGAA 
GAAGTATGGA AATGGAAAAT AGAAAAAGAA AACACGATTT ATGAACAAAT GAAACGACTA 
GGAGCCTCAC TTGACTGGAC GCGACATTTT TTTACAATGG ATCAGAATCT CTCCAAAGCT 
ATTAACGAAG CTTTTATTCG ACTCCATGAC GAGGGCTTAA TATACCGTAA AAAATGTTTA 
GTCAACTGGT CTTGTGTTCT CAAGTCAGCC ATTTCTGATG TTGAGATAGA AATGTTGCAG 
TTGAAAGGGA AGACCAAACT CGAAATTCCT GGTTATGCTA AGAAAGTACA GTTTGGAACT 
TTAACCTCAT TTGCATATCC TGTTGTAGGA ACTGATGAAA AGATCATTGT GGCCACCACT 
CGTCCAGAGA CAATATTTGG GGATGTGGCT GTGGCTGTAC ACCCTGAAGA TTCTCGTTAC 
CAACATCTTC ATGGAGCCTT ACTTCAGCAT CCATTTTCAG ATCGAAAGAT TCCAGTCATT 
GCTGATGAGT TTGTCGACAA AGAGTTTGGA GAAGGTGCTG TCAAACTGAC CCCTTGTCAT 
GATCAAACGG ACTATGAAGT TTGTGAGCGG CATGGTTTAC CAATTTCCTT CTCTATCATT 
GATGACTCTG GTCTTATGTG CAATGTTGGT GCTCCTTTTG AGGGAATGAA GAGGTTTGAT 
GCAAGAAAAG CTGTTGTAAA ATCCTTAAAA CAGAAAGACT TATACCATGG AACGATCAAC 
CATTCCATGA TGCTTCCTGT CTGCAGTCGT TCCAAAGATG TTATTGAACC TCTTCTGATG 
GACCAGTGGT TCCTCAATTG TAAAACAATG GCAACCAAAG CAACTCAGGC TGTCACCAAC 
AACCTCCTCT GCTTCATCCC TGATTACTAT GACAGTATCT GGTTCCACTG GCTGCAGAAT 
ATCAGGGACT GGTGTATTTC CAGACAATTG TGGTGGGGAC ACCAAATACC TGCTTATTTC 
GCTACCACTA CGCCGTCACC GCAGCTATCA ACCTCGAGTT CCACTGGAAT ATGGGTTAGT 
GCCAGAAACC AAGAGGAAGC TCTAGCTAAA GCCAGTGAGA AATTATCTGT TCCCAAAGAA 
CAGATAACTC TTAAACAAGA TGAAGATGTC TTGGATACTT GGTTTTCATC AGCATTGCTT 
CCATTTTCAG TATTTGGATG GCCAGAAAAA ACAATTGACT ATGAATCTTT CTACCCAACC 
GACCTTCTTG AGACTGGCAG TGATATCTTG TTCTTCTGGG TGGCCAGAAT GGTTATGCTG 
GGACAGAAGT TAACAGGACA GTTACCTTTC GATAAGATTT TATTGCATGG AATTATCCGA 
GACTCCGAAG GTAGAAAGAT GAGTAAATCT CAAGGAAATG TGATCGATCC ATTGAATGTC 
ATTTATGGTG CATCCTTAAA GGACCTTCAG AACCAACTGG ACAGTAGTGT TCTTGACCAA 
AAGGAAATTG AACATTGTAA AAAAGAGCAA CACAAAAACT TCCCACATGG TATTCCACAG 
TGTGGCGCTG ATGCTCTTAG ATTCACGCTT TGTTCTTATA ATTTCAAATT GCAAACCATC 
AACATTCAAA TGGACCATAT CAAAAGTTCA AAATTCTTTT GTAATAAAAT ATGGCAAGCT 
TCAAGATTCA TTCACCTCAA CCAAGAAGAT TCTTTCACTC CCAAGCGACC GTTCGCACTG 
TCTGGTCATG AATCTGCTAT TGATAAATGG ATCCTCAGTG TTCTCACTGA AACAACACAC 
TACTGTGACC CAAAATTCCG CAATTATGAT CTACAGTTAG TTACCAATTC CTTACGCAAG 
TTCTGGATTC AGAATTTCTG TGATATTTAT TTGGAATCTG TGAAACCAAT TTTAAAGAAT 
GGTTCAGAAG AAGAAAAAGA AACCGTGAAT CAAGTGTTAC ATTTCTGCCT GGAAACTTAC 
CTCAAAGCCA TCAGTCCTTT CATGCCTTAC CTCAGTGAGG AACTCTACCA ACAACTTATG 
CAAAGAAGCG GCCATTTTGA TGTCAGTGTT TGCAGCGCTG CTTACCCTAA CCCCAAGGAT 
TTTACCTGGC GAAATCCATT CATTGAAGAA GACATGAATA TTGTACAAGA TCTCTGCAGT 
CAGGCATTGT TTTTGAGGAA AGAATTTAGC TTGACCAAAG CTAAAGGAAA CGTTTTCATC 
AGTTCTTCCA ATGAAATCCT GCTGGATCGC CTCTCTCAGA ATTACTCTGT ACCAATATCC 
ACACTCTCAA GATCGGCTTC AGTAAACTTC ATCTATGGAA TCTTAAATAT TCCTCACGGC 
TGTGTATCAT CAAAAACAGT AGCTGACACT TGCCAAGTGC ATTTAAGTCT TCAGGGTTTA 
GTGGATCCTG TCATTGAGTT GAACAAACTC ACTAAAAAGG TGGAGAAAGT GTTGAAAGAA 
CTGGAGAAAA TTGCAGCTTC AATGAACAGA GATCTGAGAA ATAACGAACT TGTTGATGAA 
AAGAAGATAA AAGCTGTTGA AAACCTCAAC TCTGAACTTG ACCGGCTTCA GAACTATGTG 
TCCGTCTTAG AAAAACTGGA ACCCTAA