dffb cDNA ORF clone, Notothenia coriiceps(black rockcod)

Gene
Clones
gRNAs

Summary

Gene Symbol	dffb
Entrez Gene ID	104946507
Full Name	DNA fragmentation factor subunit beta
Gene Type	protein-coding
Organism	Notothenia coriiceps(black rockcod)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010772353.1	XP_010770655.1	DNA fragmentation factor subunit beta

Pathways from BioSystems
Gene Ontology

The following dffb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dffb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONo33694	XM_010772353.1 Latest version!	Notothenia coriiceps DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase) (dffb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dffb ( XM_010772353.1 ) cDNA ORF clone, Notothenia coriiceps(black rockcod) -> XP_010770655.1 Notothenia coriiceps DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase) (dffb), mRNA.

Price & Availability↑

CloneID	ONo33694
Clone ID Related Accession (Same CDS sequence)	XM_010772353.1
Accession Version	XM_010772353.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1035bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-12-22
Organism	Notothenia coriiceps(black rockcod)
Product	DNA fragmentation factor subunit beta
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011333040.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Notothenia coriiceps Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGGAC TCTTCGGGAA AAACAAACCC GTTAAAATTA GGAGTCGCAA TGAAAATAAA 
AAGTACGGAG TTGCAGCAAA AGATGTGAAA GAGTTGCTTA AAAAAGGCTG CAAGTTATTA 
CAGTTGCCAT CTTCTGGTGC ACATGTCTGT TTGTATTCTG ATGGGACAAC AGTGACGGAG 
GAATTCTTCC AAACTCTGCC GGACCACACC GAACTTGTTC TCCTCTGCAG AGATCAGACG 
TGGAGCGGAG TTGTGTGTGA CATTGGTCAG TTACTGAAGA CAGACCGATA TGCCGATGGC 
CTCATTGAAG CAGCCAAAGG GCTCCTGTTT GATGAGAAGT CTCCTCAAAG ACGTAAAATC 
CTGACCGACC TGCTGCTCAA CCTGGAGGAC AGATCTGAGC TGGAAAGCAG GGAGGAGCAT 
GAAGAATGGT TCAAAGGTGT TGATGCTCGA TTCAAGACGA AGTCGGCCTA CATGAAGTTC 
AACTGTGAGG GCAGGATACG AGGCTACATG AAGGAGGTGC ATGGGGCCAC CAAAACAATA 
CAGAAGGCTA AAGTCAGGGA AGAGTTTCTG AAAACGTCCA AAAGTCTGTC CGAAATGTTG 
AAGACGGCAA AGAACAACAG CTGCTACTTC GACAGGACCG AGAAGGAGCG GCTCTGTACT 
CGAGAAGGAT GGTTCACCTG CCAGGGATCA TATGAGCAGA AAGTGTGCCA GTCTCTCCAC 
TCCATCAACC CGTACGGCAG CAGAGAGAGC AGGATAGTGT TCAGCACATG GAACCTGGAC 
CACAGGATTG AAAAGAAGAG GACGATCATT CCCGCCCTGC TGGAAGCTCT TCAGAATCAC 
AAGAGCTCCG ACATCAACCT GAACTACTTC TACGGCCTGC TGTTCACCAG GGAGAACCTG 
AAGCTGGTGC ACATCGTGTG CCATAAGAAA GGAGCGCACA ACCTGCTATG TGACGCCAAG 
AAGATCTTTA GAAAAGCCAC TGACACAAAC AAAGCAAAAC AGAATGTGAA AGAGAAGAAG 
AGGCGCTTGG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010770655.1
CDS	33..1067
Translation	MFGLFGKNKPVKIRSRNENKKYGVAAKDVKELLKKGCKLLQLPSSGAHVCLYSDGTTVTEEFFQTLPDHTELVLLCRDQTWSGVVCDIGQLLKTDRYADGLIEAAKGLLFDEKSPQRRKILTDLLLNLEDRSELESREEHEEWFKGVDARFKTKSAYMKFNCEGRIRGYMKEVHGATKTIQKAKVREEFLKTSKSLSEMLKTAKNNSCYFDRTEKERLCTREGWFTCQGSYEQKVCQSLHSINPYGSRESRIVFSTWNLDHRIEKKRTIIPALLEALQNHKSSDINLNYFYGLLFTRENLKLVHIVCHKKGAHNLLCDAKKIFRKATDTNKAKQNVKEKKRRLV

Target ORF information:

RefSeq Version	XM_010772353.1
Organism	Notothenia coriiceps(black rockcod)
Definition	Notothenia coriiceps DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase) (dffb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010772353.1

ORF Insert Sequence:

ATGTTTGGAC TCTTCGGGAA AAACAAACCC GTTAAAATTA GGAGTCGCAA TGAAAATAAA 
AAGTACGGAG TTGCAGCAAA AGATGTGAAA GAGTTGCTTA AAAAAGGCTG CAAGTTATTA 
CAGTTGCCAT CTTCTGGTGC ACATGTCTGT TTGTATTCTG ATGGGACAAC AGTGACGGAG 
GAATTCTTCC AAACTCTGCC GGACCACACC GAACTTGTTC TCCTCTGCAG AGATCAGACG 
TGGAGCGGAG TTGTGTGTGA CATTGGTCAG TTACTGAAGA CAGACCGATA TGCCGATGGC 
CTCATTGAAG CAGCCAAAGG GCTCCTGTTT GATGAGAAGT CTCCTCAAAG ACGTAAAATC 
CTGACCGACC TGCTGCTCAA CCTGGAGGAC AGATCTGAGC TGGAAAGCAG GGAGGAGCAT 
GAAGAATGGT TCAAAGGTGT TGATGCTCGA TTCAAGACGA AGTCGGCCTA CATGAAGTTC 
AACTGTGAGG GCAGGATACG AGGCTACATG AAGGAGGTGC ATGGGGCCAC CAAAACAATA 
CAGAAGGCTA AAGTCAGGGA AGAGTTTCTG AAAACGTCCA AAAGTCTGTC CGAAATGTTG 
AAGACGGCAA AGAACAACAG CTGCTACTTC GACAGGACCG AGAAGGAGCG GCTCTGTACT 
CGAGAAGGAT GGTTCACCTG CCAGGGATCA TATGAGCAGA AAGTGTGCCA GTCTCTCCAC 
TCCATCAACC CGTACGGCAG CAGAGAGAGC AGGATAGTGT TCAGCACATG GAACCTGGAC 
CACAGGATTG AAAAGAAGAG GACGATCATT CCCGCCCTGC TGGAAGCTCT TCAGAATCAC 
AAGAGCTCCG ACATCAACCT GAACTACTTC TACGGCCTGC TGTTCACCAG GGAGAACCTG 
AAGCTGGTGC ACATCGTGTG CCATAAGAAA GGAGCGCACA ACCTGCTATG TGACGCCAAG 
AAGATCTTTA GAAAAGCCAC TGACACAAAC AAAGCAAAAC AGAATGTGAA AGAGAAGAAG 
AGGCGCTTGG TGTGA