crim1 cDNA ORF clone, Nothobranchius furzeri(turquoise killifish)

Gene
Clones
gRNAs

Summary

Gene Symbol	crim1
Entrez Gene ID	107392456
Full Name	cysteine rich transmembrane BMP regulator 1
Gene Type	protein-coding
Organism	Nothobranchius furzeri(turquoise killifish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015970272.1	XP_015825758.1	cysteine-rich motor neuron 1 protein

Pathways from BioSystems
Gene Ontology

The following crim1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the crim1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONo25516	XM_015970272.1 Latest version!	Nothobranchius furzeri cysteine rich transmembrane BMP regulator 1 (chordin-like) (crim1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

crim1 ( XM_015970272.1 ) cDNA ORF clone, Nothobranchius furzeri(turquoise killifish) -> XP_015825758.1 Nothobranchius furzeri cysteine rich transmembrane BMP regulator 1 (chordin-like) (crim1), mRNA.

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CloneID	ONo25516
Clone ID Related Accession (Same CDS sequence)	XM_015970272.1
Accession Version	XM_015970272.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2811bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-03-17
Organism	Nothobranchius furzeri(turquoise killifish)
Product	cysteine-rich motor neuron 1 protein
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015443593.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nothobranchius furzeri Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAGCA AATGCGCCGT TCTTTCACTC AACGTGCTCT TTCTGGTGCG TGCGCTCTTG 
GCGCACGAGT GCACGCCCTG TAACCTTCGG ACTTGTCCCG TCAAGCCACC ACTGCACGGC 
TGCGCGGGTG GCCGGAGCTT GGCGAAGGAT CCATGCGGAT GCTGTGATCA GTGCACGCGG 
CTGGAGTGGG AGAGGTGCGG TGGTCAGGAC TGGACGCACG GATACTGCGC TCTGGGGCTG 
ACCTGCGCTT CTGTCAATAA AACGGGAGCT GCCACCATCC CTGAGATTGG GGTGTGTAAG 
GTCATTCCTG ATCATCCAGA TGCTGGCCTC GTGGATGAGC GCTGTCCTCT AATGACGGGC 
TGCGATAGAG CTGGAAGTCA GTGTGTGTGT GATGCACGTC ACTCCTGCTT GGGCTCCTTT 
GCCTATCCAG ACCAAGAAAC CTGTCTGAAG GGCAGCAAAT CAGATGGTCG GAGACACGAT 
CATCGGGACA GGCACAGAGA AAAGTACGTC GGACCTTCCA ACCCAGCATG CTTGTTCTCT 
GGGTGTAACC TGACTGCTGA GGGCTGCGTT TGTGAGTCTC AGAGCTGCCA CCACCACTTT 
GCCTACATCA ACCGGAGCCA GTGCCAGGAA GCAGCAGAGG AGCTGAGGTG TGCCGGGGTG 
ACATGTCCTG CTTTTCCGTT GCCTTCCTGC CCCAAAGGGT CAGTCCTGAC CAAGAGTTAC 
ACACCTCCTA CTGGTTGCTG CACCTCCATA CCACCCCAGT GCACTTGTGA CCTCCGTGCC 
TGCCAGAAGC CCCACTGCCC GAGTGGTCAG CATCTCATTC CAGTCAGTCA AGCCAGTGGT 
CTGCCTGGAG ACTGCTGCGA TGTCTTCAAG TGCCAAAAAG TTTCACCAAA GTGTGTCTTC 
AATGGGAAAA ACTTCTCTGA GGGCGAAGTG TACCGCATGG ATTCCTGCTG GCTGTGTCAG 
TGTCGCGGAG GAATCTCCTT CTGCTCCAAG ACCGAATGTG CCGAGCTGGA ATGTGAAAAC 
TTCTATGTTC CTGACGGCGA GTGCTGCCCT GTCTGCATAG GTATAGAGCT GTTGTCATTA 
GACAGAACAA AAAACAACTG TTGGGTCAAT AATAAGCTGC GAGCCCATGA GGAGCAGTGG 
AAGGAGGATG ACTGCACCTT CTGCCAGTGT GTGGATGGAG AGCCTCACTG CACAGCCATG 
GCCTGCAAAC AAACCTGCCA AAACCCCGTG AAGATCCCCG GAGAGTGCTG CCCCTTCTGT 
GAAGAGCCTT CTTACGAGAC AGTTAGTCCC CTTTTATGCC CACCTCTGGA AAACTGCTCC 
TTGTCAGTGA ATGACTGCCC CTATGGCTTC CATCAAGACA AAAATGGGTG CCTCCTCTGC 
CAGTGTCTCA ACAATGATTC CTGTCCAGAA TTGGAAAAAT ACTGTTCTCT GCAATGTCCA 
ATGGGATATG AGAAGGATGA TTTTGGCTGT GAAGTGTGTG AATGCCACAT CCCAACGCCC 
AAGTGCCGAC CTCTGACCTG CACAAAGACC TGCCCTTATG GATATGTGAG GAACAAACAT 
GGCTGTGAAA TGTGTCGCTG TGTCAAATGT CCTCCCTTCA CCTGCGATCA GCACTGCAGC 
CATGGATATC GACTGAACAG AAAGGGATGC TCCATCTGCA CGTGTAAAGA AAGTGTCCCA 
ACCACCACCG CCCCAGCACC AACACCCAGC TACTGCCTCA CCTCCAATGG TCTGCACTAT 
GAGGAAGGTG CCAGCTGGCA TGACGGCTGT CGTGACTGCT ATTGTCATTC AGGAAGAGAG 
ATGTGTGTAC TTATATCCTG CCCCATACCC AGCTGCTCTC ACCCCGTTGT CAAGCCTGAT 
CAATGTTGTC CTACCTGTGA AGATGAGTCA GGAAGTGGAC AACCAGAGGG GGTGGACACG 
GTTTGCAGAG CACCTGGAGG GGAGTTTTAT GTAGAGGGTG AGACCTGGAA CCTAGACGAG 
TGCACACGCT GCACCTGCAG GGAGGGACGC GTCCTGTGCG ACACCGAAGT GTGTCCCCCG 
GCTCTTTGCC AGACACCCAT CAGGAACAAG GACACCTGTT GCCATGTGTG TCCAGAGGAG 
ACCCTGATCT CTTTGCTGCC AGTGAACAGA AGCCAGCAGC AGTACTGTAT AACCAGCGAA 
GGAGAAGTGC TAATAGCAGG AGACTCCTGG AAGGCTAACG CCTGCACCAG TTGCACCTGC 
AACAATGGCA CCATTCAGTG TTTCTCTCAG CGCTGTCCGG CTGCAAAATG CAGGGTGCCG 
GTTCTACGCA AGGGCCAGTG CTGCCCACAC TGTCTCGATT CAACAACATC GGTGCCTGTG 
ATGGTGTCCA CCACCACCTC GAAGTCGGTG GCTTCCACCA CGGTGAAGAA GGAATTTTGG 
GTTACTACGG TTACATTCCC ACTCGTCATT GCTGATACAG ATGAGCCACG TTTTGGTGAA 
AACTACCCCA GCCAAACAGA GATGGCCCTT GTTTACCAAT CGGCTGCCTG GATCCTCGCT 
GGCCTCCTCC TGGCCATCAT CATCTTCCTC ATTGTGGCCT TGCTCATCAA CAGAAAGAAG 
AAGTGGGTGC AGATGTCCTG CTATAGTGCA CCAAAAAAGA CGGTGATCCT GAAGAAGCAT 
GTGAACAAGA GCTCTGTCAT CTACATGGAA CCCTCCAAGG AGAACAAATT TCAGAACGTG 
AAGAATGACT GTGGCATCAT TCATTTTTCT CCAGAAATGA GCTCCCCATG CACAGACAAG 
ATGACCATCC CCAGGGCCAA GCTGAGCATG AACGACTGGA CGCCCCACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015825758.1
CDS	545..3355
Translation	MFSKCAVLSLNVLFLVRALLAHECTPCNLRTCPVKPPLHGCAGGRSLAKDPCGCCDQCTRLEWERCGGQDWTHGYCALGLTCASVNKTGAATIPEIGVCKVIPDHPDAGLVDERCPLMTGCDRAGSQCVCDARHSCLGSFAYPDQETCLKGSKSDGRRHDHRDRHREKYVGPSNPACLFSGCNLTAEGCVCESQSCHHHFAYINRSQCQEAAEELRCAGVTCPAFPLPSCPKGSVLTKSYTPPTGCCTSIPPQCTCDLRACQKPHCPSGQHLIPVSQASGLPGDCCDVFKCQKVSPKCVFNGKNFSEGEVYRMDSCWLCQCRGGISFCSKTECAELECENFYVPDGECCPVCIGIELLSLDRTKNNCWVNNKLRAHEEQWKEDDCTFCQCVDGEPHCTAMACKQTCQNPVKIPGECCPFCEEPSYETVSPLLCPPLENCSLSVNDCPYGFHQDKNGCLLCQCLNNDSCPELEKYCSLQCPMGYEKDDFGCEVCECHIPTPKCRPLTCTKTCPYGYVRNKHGCEMCRCVKCPPFTCDQHCSHGYRLNRKGCSICTCKESVPTTTAPAPTPSYCLTSNGLHYEEGASWHDGCRDCYCHSGREMCVLISCPIPSCSHPVVKPDQCCPTCEDESGSGQPEGVDTVCRAPGGEFYVEGETWNLDECTRCTCREGRVLCDTEVCPPALCQTPIRNKDTCCHVCPEETLISLLPVNRSQQQYCITSEGEVLIAGDSWKANACTSCTCNNGTIQCFSQRCPAAKCRVPVLRKGQCCPHCLDSTTSVPVMVSTTTSKSVASTTVKKEFWVTTVTFPLVIADTDEPRFGENYPSQTEMALVYQSAAWILAGLLLAIIIFLIVALLINRKKKWVQMSCYSAPKKTVILKKHVNKSSVIYMEPSKENKFQNVKNDCGIIHFSPEMSSPCTDKMTIPRAKLSMNDWTPH

Target ORF information:

RefSeq Version	XM_015970272.1
Organism	Nothobranchius furzeri(turquoise killifish)
Definition	Nothobranchius furzeri cysteine rich transmembrane BMP regulator 1 (chordin-like) (crim1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015970272.1

ORF Insert Sequence:

ATGTTCAGCA AATGCGCCGT TCTTTCACTC AACGTGCTCT TTCTGGTGCG TGCGCTCTTG 
GCGCACGAGT GCACGCCCTG TAACCTTCGG ACTTGTCCCG TCAAGCCACC ACTGCACGGC 
TGCGCGGGTG GCCGGAGCTT GGCGAAGGAT CCATGCGGAT GCTGTGATCA GTGCACGCGG 
CTGGAGTGGG AGAGGTGCGG TGGTCAGGAC TGGACGCACG GATACTGCGC TCTGGGGCTG 
ACCTGCGCTT CTGTCAATAA AACGGGAGCT GCCACCATCC CTGAGATTGG GGTGTGTAAG 
GTCATTCCTG ATCATCCAGA TGCTGGCCTC GTGGATGAGC GCTGTCCTCT AATGACGGGC 
TGCGATAGAG CTGGAAGTCA GTGTGTGTGT GATGCACGTC ACTCCTGCTT GGGCTCCTTT 
GCCTATCCAG ACCAAGAAAC CTGTCTGAAG GGCAGCAAAT CAGATGGTCG GAGACACGAT 
CATCGGGACA GGCACAGAGA AAAGTACGTC GGACCTTCCA ACCCAGCATG CTTGTTCTCT 
GGGTGTAACC TGACTGCTGA GGGCTGCGTT TGTGAGTCTC AGAGCTGCCA CCACCACTTT 
GCCTACATCA ACCGGAGCCA GTGCCAGGAA GCAGCAGAGG AGCTGAGGTG TGCCGGGGTG 
ACATGTCCTG CTTTTCCGTT GCCTTCCTGC CCCAAAGGGT CAGTCCTGAC CAAGAGTTAC 
ACACCTCCTA CTGGTTGCTG CACCTCCATA CCACCCCAGT GCACTTGTGA CCTCCGTGCC 
TGCCAGAAGC CCCACTGCCC GAGTGGTCAG CATCTCATTC CAGTCAGTCA AGCCAGTGGT 
CTGCCTGGAG ACTGCTGCGA TGTCTTCAAG TGCCAAAAAG TTTCACCAAA GTGTGTCTTC 
AATGGGAAAA ACTTCTCTGA GGGCGAAGTG TACCGCATGG ATTCCTGCTG GCTGTGTCAG 
TGTCGCGGAG GAATCTCCTT CTGCTCCAAG ACCGAATGTG CCGAGCTGGA ATGTGAAAAC 
TTCTATGTTC CTGACGGCGA GTGCTGCCCT GTCTGCATAG GTATAGAGCT GTTGTCATTA 
GACAGAACAA AAAACAACTG TTGGGTCAAT AATAAGCTGC GAGCCCATGA GGAGCAGTGG 
AAGGAGGATG ACTGCACCTT CTGCCAGTGT GTGGATGGAG AGCCTCACTG CACAGCCATG 
GCCTGCAAAC AAACCTGCCA AAACCCCGTG AAGATCCCCG GAGAGTGCTG CCCCTTCTGT 
GAAGAGCCTT CTTACGAGAC AGTTAGTCCC CTTTTATGCC CACCTCTGGA AAACTGCTCC 
TTGTCAGTGA ATGACTGCCC CTATGGCTTC CATCAAGACA AAAATGGGTG CCTCCTCTGC 
CAGTGTCTCA ACAATGATTC CTGTCCAGAA TTGGAAAAAT ACTGTTCTCT GCAATGTCCA 
ATGGGATATG AGAAGGATGA TTTTGGCTGT GAAGTGTGTG AATGCCACAT CCCAACGCCC 
AAGTGCCGAC CTCTGACCTG CACAAAGACC TGCCCTTATG GATATGTGAG GAACAAACAT 
GGCTGTGAAA TGTGTCGCTG TGTCAAATGT CCTCCCTTCA CCTGCGATCA GCACTGCAGC 
CATGGATATC GACTGAACAG AAAGGGATGC TCCATCTGCA CGTGTAAAGA AAGTGTCCCA 
ACCACCACCG CCCCAGCACC AACACCCAGC TACTGCCTCA CCTCCAATGG TCTGCACTAT 
GAGGAAGGTG CCAGCTGGCA TGACGGCTGT CGTGACTGCT ATTGTCATTC AGGAAGAGAG 
ATGTGTGTAC TTATATCCTG CCCCATACCC AGCTGCTCTC ACCCCGTTGT CAAGCCTGAT 
CAATGTTGTC CTACCTGTGA AGATGAGTCA GGAAGTGGAC AACCAGAGGG GGTGGACACG 
GTTTGCAGAG CACCTGGAGG GGAGTTTTAT GTAGAGGGTG AGACCTGGAA CCTAGACGAG 
TGCACACGCT GCACCTGCAG GGAGGGACGC GTCCTGTGCG ACACCGAAGT GTGTCCCCCG 
GCTCTTTGCC AGACACCCAT CAGGAACAAG GACACCTGTT GCCATGTGTG TCCAGAGGAG 
ACCCTGATCT CTTTGCTGCC AGTGAACAGA AGCCAGCAGC AGTACTGTAT AACCAGCGAA 
GGAGAAGTGC TAATAGCAGG AGACTCCTGG AAGGCTAACG CCTGCACCAG TTGCACCTGC 
AACAATGGCA CCATTCAGTG TTTCTCTCAG CGCTGTCCGG CTGCAAAATG CAGGGTGCCG 
GTTCTACGCA AGGGCCAGTG CTGCCCACAC TGTCTCGATT CAACAACATC GGTGCCTGTG 
ATGGTGTCCA CCACCACCTC GAAGTCGGTG GCTTCCACCA CGGTGAAGAA GGAATTTTGG 
GTTACTACGG TTACATTCCC ACTCGTCATT GCTGATACAG ATGAGCCACG TTTTGGTGAA 
AACTACCCCA GCCAAACAGA GATGGCCCTT GTTTACCAAT CGGCTGCCTG GATCCTCGCT 
GGCCTCCTCC TGGCCATCAT CATCTTCCTC ATTGTGGCCT TGCTCATCAA CAGAAAGAAG 
AAGTGGGTGC AGATGTCCTG CTATAGTGCA CCAAAAAAGA CGGTGATCCT GAAGAAGCAT 
GTGAACAAGA GCTCTGTCAT CTACATGGAA CCCTCCAAGG AGAACAAATT TCAGAACGTG 
AAGAATGACT GTGGCATCAT TCATTTTTCT CCAGAAATGA GCTCCCCATG CACAGACAAG 
ATGACCATCC CCAGGGCCAA GCTGAGCATG AACGACTGGA CGCCCCACTA A