PDE3B cDNA ORF clone, Neomonachus schauinslandi(Hawaiian monk seal)

Gene
Clones
gRNAs

Summary

Gene Symbol	PDE3B
Entrez Gene ID	110576583
Full Name	phosphodiesterase 3B
Gene Type	protein-coding
Organism	Neomonachus schauinslandi(Hawaiian monk seal)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021685778.1	XP_021541453.1	cGMP-inhibited 3',5'-cyclic phosphodiesterase B

Pathways from BioSystems
Gene Ontology

The following PDE3B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PDE3B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONe139524	XM_021685778.1 Latest version!	Neomonachus schauinslandi phosphodiesterase 3B (PDE3B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PDE3B ( XM_021685778.1 ) cDNA ORF clone, Neomonachus schauinslandi(Hawaiian monk seal) -> XP_021541453.1 Neomonachus schauinslandi phosphodiesterase 3B (PDE3B), mRNA.

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CloneID	ONe139524
Clone ID Related Accession (Same CDS sequence)	XM_021685778.1
Accession Version	XM_021685778.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3504bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-27
Organism	Neomonachus schauinslandi(Hawaiian monk seal)
Product	cGMP-inhibited 3',5'-cyclic phosphodiesterase B
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018734298.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Neomonachus schauinslandi Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 3% of CDS bases ##RefSeq-Attributes-END##

ATGGTCTTGG AGAGGACGCC GAATCCTCCC CTCTGCCCTT CTCTAGAACA CGCTGTTAAC 
AGCGAGAAAG ACTTCAAGGT GGAACAACTT TGTGACAACA GATTTTCTGT GCTTGTTCAG 
GGAGACACAG ATGTGAACCA GAGGGACGAG CGAGACGCCA AAGCCATGCG GTGCCCGCAG 
CCGCCGGACG GGGCCGCCTC CTCCCCCGAG AGTCTGAGGA ACGGCTACGT GAAGAGCTGC 
GTGAGCCCCC TGCGGCAGGA CCCTCCGCGC GGCTTCTTCT TCCACCTCTG CCGCTTCTGC 
AACGTGGAGC TGCTGCTGCC GCCACCGGCC TCCCCCCAGC AGCCGCGCCG CGGCTCCCCC 
TCCGCCCGGG CGCGCCTCTC GCTGGGCGCC CTGGCCGCCT TTGTCCTCGC CCTGCTGCTC 
GGCTCGGGGC CCGGGAGCTG GGCTGCCGGG GCCGCCCGGC TGCGGACCCT GCTGAGCGTG 
TGCTCTCACA GCCTCAGCCC CCTGTTCAGC ATCGCCTGTG CCTTTTTCTT CCTCACCTGC 
TTCTTGACCC GGAGCAAGCG GGGCCCCGGG CCGGGCCGGA GCGGCGGCGG CTCCTGGTGG 
CTGCTGGCGC TGCCCGCCTG CTGTTACCTG GGGGACTTCT TGGTGTGGCA GGCGTGGGCT 
TGGGCTTGGG GGTCCGGAGA CGCGGGGGCC TCCGCCGCGG CCCCGCACAC GCCCCCGGCG 
GTGGCGGGCA GGTTGTTCTT GGTGCTGAGC TGCGTGGGCT TGCTGACGCT CGCGCACCCG 
GCGAGGCTCC GGCACAGCAT CCTGGTGCTG CTCTTCTCCA GCTTCGTCTG GTGGGTCTCC 
TTCACCGGCC TCGGGTCTCT GCCCGCTGCC CTCAGGCCGC TGCTCTCCTG CCTGGTCGGG 
GACGCCGGCT GCCTGCTAGC CCTGGGCTTG GATCACTTCT TTCAGATCAG GGAAGCCCCT 
CACCATCCTC GCTTGGCCAG TGCCTCCGAG GACAAAGTGC CTGTGATCAG ACCCCGCAGG 
AGGTCCAGCT GCGTGTCGTT CGGAGAAACT TCGGCCGGTT ACTCCGGCAG TTGCAAGATG 
TTCAGGAGAC CTTCGTTGCC TTGCATTTCC CGAGAACAGA TGATTCTTTG GGACTGGGAC 
TTAAAGCAAT GGTATAAACC TCATTATCAG AATTCTGGAG GTGGAAGTGG AGTGGATCTT 
TCAGTGCTAA ATGAGGCTCG CAATATGGTG TCAGATCTGC TGATCGATCC AACCCTTCCC 
CCACAAGTTA TTTCCTCCCT GCGGAGTATC AGTAGCTTGA TGGGTGCTTT CTCAGGTTCC 
TGTAGGCCAA AGCTTAATCC TCTCATGCCA TTTCCTGGAT TTTACCCCTG CTCAGAAATA 
GAGGACCCAA CTGAGAAAGG AGATCGAAAA CTTCACAAGG GACTGAACAG TAGGAGTAGT 
TTACCAACTC CACAGCTGAG GAGGAGTTCC GCAACCTCAG GTCTGCCTCC CGTTGAACAG 
TCTTCAAGGT GGGAACGCAA TAATGGCAAG AGGCCACACC AAGACTTTGG CATTTCAAGT 
CAAGGATCCT ATCTAAATGG GCCTTTTAGT TCAAGTCTAC TGACAATCGC GAAGCAAAGG 
TCATCTTCTG TATCCCTAAC TCACCATGTA GGTCTGAGAA GAGCTGGTGC TTTCCCCAGC 
CTGAGTCCTG TGAATTCTCC CAGCCATGGA CCAGTGTCTC CTGGCTCTCT AACCAGTCGA 
TCACACATAG AATTTCCTGA CACTGCCAAT TTTCTTACTA AGCCAAGTGT TATTTTACAC 
AGATCTCTGG GCAGTGCACC CAATTCACCA GATACTTATC AGCAACTCAG AAATTCTGAT 
AGCAGTTTGT GTAACAGCTG TGGACATCAA ATATTGAAAT ACGTGTCAAC ATCCGAATCT 
GAGTCTGGTA CAGACTGCCA TGGTGGAAAG TCAGGTGACG AAGATAGCAC CATTCCCTCA 
AAACAATCAT TCAACTGCAC AGAAGCGCAG GAAGTGGAAA CAGAGAACAG AGACTACAGA 
AAATTATTTT TGGAAGGTGA TAATCACCTA ACAGAAGAGG CACAGAATGA ACAGCAATCA 
AATATTGAAC AGGAAGCATC ACTGGATCTG ATTTTAATAG AAGATTATGA TTCCTTAATA 
GAAAAGATGA GCAACTGGAA TTTTCAAATA TTTGAAGTTG TAGAAAAAAT GGGAGAGAAA 
TCAGGAAGGA TCCTTAGTCA GGTTATGTAT ACCTTATTTC AAGACACTGG CTTATTGGAA 
ATATTTAAAA TCCCCACTCT ACAGTTCATG AACTATTTTC GTGCATTGGA GAATGGCTAC 
CGAGACATTC CTTATCATAA TCGCATACAC GCCACAGATG TCCTGCATGC AGTTTGGTAT 
CTGACAACGC GGCCAGTTCC TGGTTTACAG CAGATCCACA ATGACCACGC AGCAGGAAAT 
GAAACAGATT CCGATGGCAG AATGAACCCT GGGCAGTTGG CTTACATTTC TTCGAGGAGC 
TGCTCTATTC CAGACGAGAG CTATGGTTGC CTTTCTTCAA ACATTCCTGC GCTGGAATTG 
ATGGCTCTCT ATGTGGCAGC CGCCATGCAT GATTACGATC ACCCAGGGCG GACAAATGCA 
TTTCTAGTGG CTACAAATGC CCCTCAGGCA GTTTTGTACA ATGACAGATC TGTTCTAGAA 
AATCATCATG CTGCATCAGC TTGGAATCTG TATCTCTCTC GCCCAGAATA CAACTTCCTT 
CTTAATCTTG ATCATGTGGA ATTCAAGCGC TTTCGTTTTT TGGTCATTGA AGCAATCCTT 
GCCACTGATC TTAAAAAGCA TTTTGATTTC CTTGCTGAAT TCAACGCCAA GGCCAACGAT 
GTAAATAGTA ATGGCATAGA ATGGAGCAAT GAGAATGACC GCCTCTTAGT ATGCCAGGTG 
TGCATCAAGC TGGCAGACAT CAACGGCCCG GCGAAAGTTC GGGACTTGCA TTTGAAATGG 
ACAGAAGGCA TTGTCAATGA ATTTTATGAG CAGGGAGATG AGGAAGCAAA CCTCGGTTTG 
CCCATCAGTC CCTTTATGGA TCGTTCTTCG CCTCAGCTAG CAAAACTCCA AGAATCTTTC 
ATCACCCACA TCGTGGGTCC TCTGTGTAAT TCCTACGACG CGGCTGGCTT GCTGCCGGGT 
CAGTGGGTAG AAGCAGAAGA GGACGAGGAC ACCGAGAGTT GTTACGACGA CGAAGATGGT 
GAAGAATTAG ATACAGATGA TGAAGAAATA GAAGACAGTC TAAATCCTAA ACCACGAAGG 
AGGAAAGGCA GACGGCGAAT ATTTTGTCAG CTAATGCACC ACCTCACTGA AAACCACAAG 
ATATGGAAGG AAATGATAGA GGAAGAAGAG AAATGTAAAG CTGATGGGAA TAAACTGCAG 
GTGGAAACTC CTTCCTTACC TCAAGCAGAT GACATTCAGG TCATTGAAGA AGCAGATGAA 
GAGGAGGAGA GACAGTTTGA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021541453.1
CDS	1..3504
Translation	MVLERTPNPPLCPSLEHAVNSEKDFKVEQLCDNRFSVLVQGDTDVNQRDERDAKAMRCPQPPDGAASSPESLRNGYVKSCVSPLRQDPPRGFFFHLCRFCNVELLLPPPASPQQPRRGSPSARARLSLGALAAFVLALLLGSGPGSWAAGAARLRTLLSVCSHSLSPLFSIACAFFFLTCFLTRSKRGPGPGRSGGGSWWLLALPACCYLGDFLVWQAWAWAWGSGDAGASAAAPHTPPAVAGRLFLVLSCVGLLTLAHPARLRHSILVLLFSSFVWWVSFTGLGSLPAALRPLLSCLVGDAGCLLALGLDHFFQIREAPHHPRLASASEDKVPVIRPRRRSSCVSFGETSAGYSGSCKMFRRPSLPCISREQMILWDWDLKQWYKPHYQNSGGGSGVDLSVLNEARNMVSDLLIDPTLPPQVISSLRSISSLMGAFSGSCRPKLNPLMPFPGFYPCSEIEDPTEKGDRKLHKGLNSRSSLPTPQLRRSSATSGLPPVEQSSRWERNNGKRPHQDFGISSQGSYLNGPFSSSLLTIAKQRSSSVSLTHHVGLRRAGAFPSLSPVNSPSHGPVSPGSLTSRSHIEFPDTANFLTKPSVILHRSLGSAPNSPDTYQQLRNSDSSLCNSCGHQILKYVSTSESESGTDCHGGKSGDEDSTIPSKQSFNCTEAQEVETENRDYRKLFLEGDNHLTEEAQNEQQSNIEQEASLDLILIEDYDSLIEKMSNWNFQIFEVVEKMGEKSGRILSQVMYTLFQDTGLLEIFKIPTLQFMNYFRALENGYRDIPYHNRIHATDVLHAVWYLTTRPVPGLQQIHNDHAAGNETDSDGRMNPGQLAYISSRSCSIPDESYGCLSSNIPALELMALYVAAAMHDYDHPGRTNAFLVATNAPQAVLYNDRSVLENHHAASAWNLYLSRPEYNFLLNLDHVEFKRFRFLVIEAILATDLKKHFDFLAEFNAKANDVNSNGIEWSNENDRLLVCQVCIKLADINGPAKVRDLHLKWTEGIVNEFYEQGDEEANLGLPISPFMDRSSPQLAKLQESFITHIVGPLCNSYDAAGLLPGQWVEAEEDEDTESCYDDEDGEELDTDDEEIEDSLNPKPRRRKGRRRIFCQLMHHLTENHKIWKEMIEEEEKCKADGNKLQVETPSLPQADDIQVIEEADEEEERQFE

Target ORF information:

RefSeq Version	XM_021685778.1
Organism	Neomonachus schauinslandi(Hawaiian monk seal)
Definition	Neomonachus schauinslandi phosphodiesterase 3B (PDE3B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021685778.1

ORF Insert Sequence:

ATGGTCTTGG AGAGGACGCC GAATCCTCCC CTCTGCCCTT CTCTAGAACA CGCTGTTAAC 
AGCGAGAAAG ACTTCAAGGT GGAACAACTT TGTGACAACA GATTTTCTGT GCTTGTTCAG 
GGAGACACAG ATGTGAACCA GAGGGACGAG CGAGACGCCA AAGCCATGCG GTGCCCGCAG 
CCGCCGGACG GGGCCGCCTC CTCCCCCGAG AGTCTGAGGA ACGGCTACGT GAAGAGCTGC 
GTGAGCCCCC TGCGGCAGGA CCCTCCGCGC GGCTTCTTCT TCCACCTCTG CCGCTTCTGC 
AACGTGGAGC TGCTGCTGCC GCCACCGGCC TCCCCCCAGC AGCCGCGCCG CGGCTCCCCC 
TCCGCCCGGG CGCGCCTCTC GCTGGGCGCC CTGGCCGCCT TTGTCCTCGC CCTGCTGCTC 
GGCTCGGGGC CCGGGAGCTG GGCTGCCGGG GCCGCCCGGC TGCGGACCCT GCTGAGCGTG 
TGCTCTCACA GCCTCAGCCC CCTGTTCAGC ATCGCCTGTG CCTTTTTCTT CCTCACCTGC 
TTCTTGACCC GGAGCAAGCG GGGCCCCGGG CCGGGCCGGA GCGGCGGCGG CTCCTGGTGG 
CTGCTGGCGC TGCCCGCCTG CTGTTACCTG GGGGACTTCT TGGTGTGGCA GGCGTGGGCT 
TGGGCTTGGG GGTCCGGAGA CGCGGGGGCC TCCGCCGCGG CCCCGCACAC GCCCCCGGCG 
GTGGCGGGCA GGTTGTTCTT GGTGCTGAGC TGCGTGGGCT TGCTGACGCT CGCGCACCCG 
GCGAGGCTCC GGCACAGCAT CCTGGTGCTG CTCTTCTCCA GCTTCGTCTG GTGGGTCTCC 
TTCACCGGCC TCGGGTCTCT GCCCGCTGCC CTCAGGCCGC TGCTCTCCTG CCTGGTCGGG 
GACGCCGGCT GCCTGCTAGC CCTGGGCTTG GATCACTTCT TTCAGATCAG GGAAGCCCCT 
CACCATCCTC GCTTGGCCAG TGCCTCCGAG GACAAAGTGC CTGTGATCAG ACCCCGCAGG 
AGGTCCAGCT GCGTGTCGTT CGGAGAAACT TCGGCCGGTT ACTCCGGCAG TTGCAAGATG 
TTCAGGAGAC CTTCGTTGCC TTGCATTTCC CGAGAACAGA TGATTCTTTG GGACTGGGAC 
TTAAAGCAAT GGTATAAACC TCATTATCAG AATTCTGGAG GTGGAAGTGG AGTGGATCTT 
TCAGTGCTAA ATGAGGCTCG CAATATGGTG TCAGATCTGC TGATCGATCC AACCCTTCCC 
CCACAAGTTA TTTCCTCCCT GCGGAGTATC AGTAGCTTGA TGGGTGCTTT CTCAGGTTCC 
TGTAGGCCAA AGCTTAATCC TCTCATGCCA TTTCCTGGAT TTTACCCCTG CTCAGAAATA 
GAGGACCCAA CTGAGAAAGG AGATCGAAAA CTTCACAAGG GACTGAACAG TAGGAGTAGT 
TTACCAACTC CACAGCTGAG GAGGAGTTCC GCAACCTCAG GTCTGCCTCC CGTTGAACAG 
TCTTCAAGGT GGGAACGCAA TAATGGCAAG AGGCCACACC AAGACTTTGG CATTTCAAGT 
CAAGGATCCT ATCTAAATGG GCCTTTTAGT TCAAGTCTAC TGACAATCGC GAAGCAAAGG 
TCATCTTCTG TATCCCTAAC TCACCATGTA GGTCTGAGAA GAGCTGGTGC TTTCCCCAGC 
CTGAGTCCTG TGAATTCTCC CAGCCATGGA CCAGTGTCTC CTGGCTCTCT AACCAGTCGA 
TCACACATAG AATTTCCTGA CACTGCCAAT TTTCTTACTA AGCCAAGTGT TATTTTACAC 
AGATCTCTGG GCAGTGCACC CAATTCACCA GATACTTATC AGCAACTCAG AAATTCTGAT 
AGCAGTTTGT GTAACAGCTG TGGACATCAA ATATTGAAAT ACGTGTCAAC ATCCGAATCT 
GAGTCTGGTA CAGACTGCCA TGGTGGAAAG TCAGGTGACG AAGATAGCAC CATTCCCTCA 
AAACAATCAT TCAACTGCAC AGAAGCGCAG GAAGTGGAAA CAGAGAACAG AGACTACAGA 
AAATTATTTT TGGAAGGTGA TAATCACCTA ACAGAAGAGG CACAGAATGA ACAGCAATCA 
AATATTGAAC AGGAAGCATC ACTGGATCTG ATTTTAATAG AAGATTATGA TTCCTTAATA 
GAAAAGATGA GCAACTGGAA TTTTCAAATA TTTGAAGTTG TAGAAAAAAT GGGAGAGAAA 
TCAGGAAGGA TCCTTAGTCA GGTTATGTAT ACCTTATTTC AAGACACTGG CTTATTGGAA 
ATATTTAAAA TCCCCACTCT ACAGTTCATG AACTATTTTC GTGCATTGGA GAATGGCTAC 
CGAGACATTC CTTATCATAA TCGCATACAC GCCACAGATG TCCTGCATGC AGTTTGGTAT 
CTGACAACGC GGCCAGTTCC TGGTTTACAG CAGATCCACA ATGACCACGC AGCAGGAAAT 
GAAACAGATT CCGATGGCAG AATGAACCCT GGGCAGTTGG CTTACATTTC TTCGAGGAGC 
TGCTCTATTC CAGACGAGAG CTATGGTTGC CTTTCTTCAA ACATTCCTGC GCTGGAATTG 
ATGGCTCTCT ATGTGGCAGC CGCCATGCAT GATTACGATC ACCCAGGGCG GACAAATGCA 
TTTCTAGTGG CTACAAATGC CCCTCAGGCA GTTTTGTACA ATGACAGATC TGTTCTAGAA 
AATCATCATG CTGCATCAGC TTGGAATCTG TATCTCTCTC GCCCAGAATA CAACTTCCTT 
CTTAATCTTG ATCATGTGGA ATTCAAGCGC TTTCGTTTTT TGGTCATTGA AGCAATCCTT 
GCCACTGATC TTAAAAAGCA TTTTGATTTC CTTGCTGAAT TCAACGCCAA GGCCAACGAT 
GTAAATAGTA ATGGCATAGA ATGGAGCAAT GAGAATGACC GCCTCTTAGT ATGCCAGGTG 
TGCATCAAGC TGGCAGACAT CAACGGCCCG GCGAAAGTTC GGGACTTGCA TTTGAAATGG 
ACAGAAGGCA TTGTCAATGA ATTTTATGAG CAGGGAGATG AGGAAGCAAA CCTCGGTTTG 
CCCATCAGTC CCTTTATGGA TCGTTCTTCG CCTCAGCTAG CAAAACTCCA AGAATCTTTC 
ATCACCCACA TCGTGGGTCC TCTGTGTAAT TCCTACGACG CGGCTGGCTT GCTGCCGGGT 
CAGTGGGTAG AAGCAGAAGA GGACGAGGAC ACCGAGAGTT GTTACGACGA CGAAGATGGT 
GAAGAATTAG ATACAGATGA TGAAGAAATA GAAGACAGTC TAAATCCTAA ACCACGAAGG 
AGGAAAGGCA GACGGCGAAT ATTTTGTCAG CTAATGCACC ACCTCACTGA AAACCACAAG 
ATATGGAAGG AAATGATAGA GGAAGAAGAG AAATGTAAAG CTGATGGGAA TAAACTGCAG 
GTGGAAACTC CTTCCTTACC TCAAGCAGAT GACATTCAGG TCATTGAAGA AGCAGATGAA 
GAGGAGGAGA GACAGTTTGA ATAA