NECAME_08404 cDNA ORF clone, Necator americanus

Gene
Clones
gRNAs

Summary

Gene Symbol	NECAME_08404
Entrez Gene ID	25348434
Full Name	trypsin
Gene Type	protein-coding
Organism	Necator americanus

mRNA and Protein(s)

mRNA	Protein	Name
XM_013448379.1	XP_013303833.1	trypsin

Pathways from BioSystems
Gene Ontology

The following NECAME_08404 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NECAME_08404 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONe131814	XM_013448379.1 Latest version!	Necator americanus trypsin mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NECAME_08404 ( XM_013448379.1 ) cDNA ORF clone, Necator americanus -> XP_013303833.1 Necator americanus trypsin mRNA.

Price & Availability↑

CloneID	ONe131814
Clone ID Related Accession (Same CDS sequence)	XM_013448379.1
Accession Version	XM_013448379.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 978bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-08-11
Organism	Necator americanus
Product	trypsin
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_013561247). COMPLETENESS: incomplete on both ends.

ATGAAAAATG AAAAGGAGAT CAGTATCTCT TCACAGCCTT CCAGCGGCAC CGGGGGTGAC 
TCACCAGTCG TCGTTATTGA ACGGCGAATC AAGGATGAGC AAACATTTCC GGATAACGGT 
TACTGTAAGC CTGGTTTCAC CTTGAAATCG TCCGGACTTT CAAGCAGGAG AAGATTAGGA 
AAAGGTCCAT CATTTGTTAT AATGGGAGGA AAACTGGTGA ATGCGAACGA ATATCCGTTT 
GCTGTTCATT TAGATAATAA TGGAGCGTTC TGCTCTGGTG TACTAATATC AAAACACTAT 
GTGCTGACAG TCGCTCACTG CATTGTAACC CTGGAAAATA TTAAACAAAG GAATCAAATT 
TGTAAAGGAG GACACATTAA ACCAGAAAGA AATGGGAAAA TTTTCATCGA ACCACAGTCT 
CTGAAAATTT ATCCAGGATC CAGATGCCCT AAGCCGGAAT TTTGCAAACC TAAGAGAACT 
GTATACAAGA GTATATCGGC ATATCCTCAT CCAGAATTTA ACCTTTGCAA TCTTGAAAAC 
GATATAGCAC TAATTGAACT CGAGGGAGAT GTCTCAGAGG AAGACGGAGC ACCCATCTGT 
TTGCCGGAGA AGGACGAAAA ACTTTCTGGA ACCTTATCCA CGGTCGGGTA CGGGTATGAC 
CCAATGGCCA AAGATCCGCT GGTGCCCGCA TTGCAAGTAG TGAGGTTCAA GAAATACAGT 
GAGAAGAATG GTGTAATTAT CACTATTGAT CCGAGAAGGG ATTCGTGCAA GGGTGACAGT 
GGCGGTCCAC TGTTTAAGAA CAAGAATGGC TTATTAACAC TGAAAGAAGA GGCCACTACA 
CTGACGTGCG AAAACACGTT AATTGGATTT GTGAAAATTC AAATGTTTGC CATAACATCG 
GTGACGATCG ATCCGGATCC GAAGAATACG AGGATAACGA TGAAGCCGAG GACTAGAGAG 
GCAGCAATGG TGGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013303833.1
CDS	1..978
Translation	MKNEKEISISSQPSSGTGGDSPVVVIERRIKDEQTFPDNGYCKPGFTLKSSGLSSRRRLGKGPSFVIMGGKLVNANEYPFAVHLDNNGAFCSGVLISKHYVLTVAHCIVTLENIKQRNQICKGGHIKPERNGKIFIEPQSLKIYPGSRCPKPEFCKPKRTVYKSISAYPHPEFNLCNLENDIALIELEGDVSEEDGAPICLPEKDEKLSGTLSTVGYGYDPMAKDPLVPALQVVRFKKYSEKNGVIITIDPRRDSCKGDSGGPLFKNKNGLLTLKEEATTLTCENTLIGFVKIQMFAITSVTIDPDPKNTRITMKPRTREAAMVD

Target ORF information:

RefSeq Version	XM_013448379.1
Organism	Necator americanus
Definition	Necator americanus trypsin mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013448379.1

ORF Insert Sequence:

ATGAAAAATG AAAAGGAGAT CAGTATCTCT TCACAGCCTT CCAGCGGCAC CGGGGGTGAC 
TCACCAGTCG TCGTTATTGA ACGGCGAATC AAGGATGAGC AAACATTTCC GGATAACGGT 
TACTGTAAGC CTGGTTTCAC CTTGAAATCG TCCGGACTTT CAAGCAGGAG AAGATTAGGA 
AAAGGTCCAT CATTTGTTAT AATGGGAGGA AAACTGGTGA ATGCGAACGA ATATCCGTTT 
GCTGTTCATT TAGATAATAA TGGAGCGTTC TGCTCTGGTG TACTAATATC AAAACACTAT 
GTGCTGACAG TCGCTCACTG CATTGTAACC CTGGAAAATA TTAAACAAAG GAATCAAATT 
TGTAAAGGAG GACACATTAA ACCAGAAAGA AATGGGAAAA TTTTCATCGA ACCACAGTCT 
CTGAAAATTT ATCCAGGATC CAGATGCCCT AAGCCGGAAT TTTGCAAACC TAAGAGAACT 
GTATACAAGA GTATATCGGC ATATCCTCAT CCAGAATTTA ACCTTTGCAA TCTTGAAAAC 
GATATAGCAC TAATTGAACT CGAGGGAGAT GTCTCAGAGG AAGACGGAGC ACCCATCTGT 
TTGCCGGAGA AGGACGAAAA ACTTTCTGGA ACCTTATCCA CGGTCGGGTA CGGGTATGAC 
CCAATGGCCA AAGATCCGCT GGTGCCCGCA TTGCAAGTAG TGAGGTTCAA GAAATACAGT 
GAGAAGAATG GTGTAATTAT CACTATTGAT CCGAGAAGGG ATTCGTGCAA GGGTGACAGT 
GGCGGTCCAC TGTTTAAGAA CAAGAATGGC TTATTAACAC TGAAAGAAGA GGCCACTACA 
CTGACGTGCG AAAACACGTT AATTGGATTT GTGAAAATTC AAATGTTTGC CATAACATCG 
GTGACGATCG ATCCGGATCC GAAGAATACG AGGATAACGA TGAAGCCGAG GACTAGAGAG 
GCAGCAATGG TGGACTAA