NECAME_06717 cDNA ORF clone, Necator americanus

Gene
Clones
gRNAs

Summary

Gene Symbol	NECAME_06717
Entrez Gene ID	25346749
Full Name	transcription initiation factor IIF, beta subunit
Gene Type	protein-coding
Organism	Necator americanus

mRNA and Protein(s)

mRNA	Protein	Name
XM_013451526.1	XP_013306980.1	transcription initiation factor IIF, beta subunit

Pathways from BioSystems
Gene Ontology

The following NECAME_06717 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NECAME_06717 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONe134462	XM_013451526.1 Latest version!	Necator americanus transcription initiation factor IIF, beta subunit mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NECAME_06717 ( XM_013451526.1 ) cDNA ORF clone, Necator americanus -> XP_013306980.1 Necator americanus transcription initiation factor IIF, beta subunit mRNA.

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CloneID	ONe134462
Clone ID Related Accession (Same CDS sequence)	XM_013451526.1
Accession Version	XM_013451526.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1407bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-08-11
Organism	Necator americanus
Product	transcription initiation factor IIF, beta subunit
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_013562062). COMPLETENESS: incomplete on both ends.

ATGACTCCGC GTAGAGACAA CGTCGATGAC GTAGAGGAAG AAGATCTGAG AGCTTATGAA 
GTAGACGGAT GGAGGGTCGG GTACGAAGTT GTGATGAACG GAGTCGGCTT CGCAGAAGTA 
AGAGAAGACT CTTCTGATTC GGATGATGAA TCAGTTGATG ATAGTGGCGA AACAACAGAA 
GCATCTCCTT ATCCTGAGTA TCGAACGCTT CCAATGCAAA TCGAGGAACC TGTCGCGGGA 
AATGCTCGTA CCAGCAAAGA TTCCGAAGAG GATTACTCTC ATTTTGAAAA TTCAAATTTG 
TCAGTGAGTG AAGTCATATC CGCCGTGGAT ACTAATGCGA CCCCTTCGAT TACAATGGAG 
TTTGAGCAGT GTTTGCGAGA CAGTTTATCC TCTTCGGAAA TATCTTCCCA TGAAGAAATT 
CCACATATGC CGACCAATAT AACTTTGGAC TCTGAGAAGA TCGAAGCAAT TCGCTCAGCA 
ATGTCAGGAT TAAGTCTCCC CCCTCCACCA CATATGGCTC CAGAGTTTAA AAATGCAAGA 
GTAAATCCCT TCTCAGTGCC TTTCTCAAAG AATGTCCTCC ATTTTCGGGT TGTGCAGTTA 
TTTGTCCATT TTAGTCTCGC TAGTTTTTCT GTGAACATGT CTCGAAAACG TGAACATGCA 
GTGGATTGTG AAATGGCCAA ACGCGGAGTA TGGCTCGTAA AAGTGCCCCG GTACTTATCC 
GATCTTTGGG AGACAAATGC TGGTACTGAT ATTGGACGAC TAGTGATACA TCAGGGTGCA 
AACGGAAAAG GAGATGTGAA GCTAAAGTCA CGTCCTGGTC TTGTATTACC ACAATCGGTA 
TCATCTACCG TTAAAGGCCC TGCGCAGATT CCTGATGAAC ATTCCTTCAT TCTCGGTGAT 
GTTCTAAATC AGACGATGGC AGTTCTGGCA GAAGACAAGA CTGGTCTCAA CGAAGAAGCA 
GAAATCAAGT CGGGTCGGTT ATCACTCGAA GGGAGGGTTG TAAAACGTGC TGAATGTCGA 
CCACCTGCCT CAAATAGTTA TTTAAAGATG AAGATTGCTC AAATCTCTAA CAGTGGACAA 
CCCAAAAAGC AGGTTTTGCA GATGGAAAAG GCAGCTGTTA AGTTTAAACC TGTTGCTGCA 
CATGCTGAAG ACATGATGCG TATCAAACAG AAAAAAGAAG GTGCCAAGAC AGTGAGAGCT 
GACCGTAACG TACTAATGCA AGCGCTGTTC CATGCATTCG AAAAGCATCA ATACTACAGA 
TTACAAGATT TACAGCAGTT GACACAGCAG CCTGCTGGGT ACGTAAAAGA ATTGTTGACT 
GAAATAGCTG TGTATAACAC TGCACCACCT CATAAAAGCA TGTGGGAGCT GAAGCCAGAA 
TATCGAGATT ATGCTGTACA GAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013306980.1
CDS	1..1407
Translation	MTPRRDNVDDVEEEDLRAYEVDGWRVGYEVVMNGVGFAEVREDSSDSDDESVDDSGETTEASPYPEYRTLPMQIEEPVAGNARTSKDSEEDYSHFENSNLSVSEVISAVDTNATPSITMEFEQCLRDSLSSSEISSHEEIPHMPTNITLDSEKIEAIRSAMSGLSLPPPPHMAPEFKNARVNPFSVPFSKNVLHFRVVQLFVHFSLASFSVNMSRKREHAVDCEMAKRGVWLVKVPRYLSDLWETNAGTDIGRLVIHQGANGKGDVKLKSRPGLVLPQSVSSTVKGPAQIPDEHSFILGDVLNQTMAVLAEDKTGLNEEAEIKSGRLSLEGRVVKRAECRPPASNSYLKMKIAQISNSGQPKKQVLQMEKAAVKFKPVAAHAEDMMRIKQKKEGAKTVRADRNVLMQALFHAFEKHQYYRLQDLQQLTQQPAGYVKELLTEIAVYNTAPPHKSMWELKPEYRDYAVQK

Target ORF information:

RefSeq Version	XM_013451526.1
Organism	Necator americanus
Definition	Necator americanus transcription initiation factor IIF, beta subunit mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013451526.1

ORF Insert Sequence:

ATGACTCCGC GTAGAGACAA CGTCGATGAC GTAGAGGAAG AAGATCTGAG AGCTTATGAA 
GTAGACGGAT GGAGGGTCGG GTACGAAGTT GTGATGAACG GAGTCGGCTT CGCAGAAGTA 
AGAGAAGACT CTTCTGATTC GGATGATGAA TCAGTTGATG ATAGTGGCGA AACAACAGAA 
GCATCTCCTT ATCCTGAGTA TCGAACGCTT CCAATGCAAA TCGAGGAACC TGTCGCGGGA 
AATGCTCGTA CCAGCAAAGA TTCCGAAGAG GATTACTCTC ATTTTGAAAA TTCAAATTTG 
TCAGTGAGTG AAGTCATATC CGCCGTGGAT ACTAATGCGA CCCCTTCGAT TACAATGGAG 
TTTGAGCAGT GTTTGCGAGA CAGTTTATCC TCTTCGGAAA TATCTTCCCA TGAAGAAATT 
CCACATATGC CGACCAATAT AACTTTGGAC TCTGAGAAGA TCGAAGCAAT TCGCTCAGCA 
ATGTCAGGAT TAAGTCTCCC CCCTCCACCA CATATGGCTC CAGAGTTTAA AAATGCAAGA 
GTAAATCCCT TCTCAGTGCC TTTCTCAAAG AATGTCCTCC ATTTTCGGGT TGTGCAGTTA 
TTTGTCCATT TTAGTCTCGC TAGTTTTTCT GTGAACATGT CTCGAAAACG TGAACATGCA 
GTGGATTGTG AAATGGCCAA ACGCGGAGTA TGGCTCGTAA AAGTGCCCCG GTACTTATCC 
GATCTTTGGG AGACAAATGC TGGTACTGAT ATTGGACGAC TAGTGATACA TCAGGGTGCA 
AACGGAAAAG GAGATGTGAA GCTAAAGTCA CGTCCTGGTC TTGTATTACC ACAATCGGTA 
TCATCTACCG TTAAAGGCCC TGCGCAGATT CCTGATGAAC ATTCCTTCAT TCTCGGTGAT 
GTTCTAAATC AGACGATGGC AGTTCTGGCA GAAGACAAGA CTGGTCTCAA CGAAGAAGCA 
GAAATCAAGT CGGGTCGGTT ATCACTCGAA GGGAGGGTTG TAAAACGTGC TGAATGTCGA 
CCACCTGCCT CAAATAGTTA TTTAAAGATG AAGATTGCTC AAATCTCTAA CAGTGGACAA 
CCCAAAAAGC AGGTTTTGCA GATGGAAAAG GCAGCTGTTA AGTTTAAACC TGTTGCTGCA 
CATGCTGAAG ACATGATGCG TATCAAACAG AAAAAAGAAG GTGCCAAGAC AGTGAGAGCT 
GACCGTAACG TACTAATGCA AGCGCTGTTC CATGCATTCG AAAAGCATCA ATACTACAGA 
TTACAAGATT TACAGCAGTT GACACAGCAG CCTGCTGGGT ACGTAAAAGA ATTGTTGACT 
GAAATAGCTG TGTATAACAC TGCACCACCT CATAAAAGCA TGTGGGAGCT GAAGCCAGAA 
TATCGAGATT ATGCTGTACA GAAGTAA