ALPL cDNA ORF clone, Nanorana parkeri

Gene
Clones
gRNAs

Summary

Gene Symbol	ALPL
Entrez Gene ID	108802475
Full Name	alkaline phosphatase, liver/bone/kidney
Gene Type	protein-coding
Organism	Nanorana parkeri

mRNA and Protein(s)

mRNA	Protein	Name
XM_018574334.1	XP_018429836.1	alkaline phosphatase, tissue-nonspecific isozyme

Pathways from BioSystems
Gene Ontology

The following ALPL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ALPL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONa69400	XM_018574334.1 Latest version!	Nanorana parkeri alkaline phosphatase, liver/bone/kidney (ALPL), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ALPL ( XM_018574334.1 ) cDNA ORF clone, Nanorana parkeri -> XP_018429836.1 Nanorana parkeri alkaline phosphatase, liver/bone/kidney (ALPL), mRNA.

Price & Availability↑

CloneID	ONa69400
Clone ID Related Accession (Same CDS sequence)	XM_018574334.1
Accession Version	XM_018574334.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1410bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-29
Organism	Nanorana parkeri
Product	alkaline phosphatase, tissue-nonspecific isozyme
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017309157.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nanorana parkeri Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTCTCG GAGACGGGAT GGGGGTGCCA ACTGTCACTG CCGCTCGGAT CTTAAAGGGC 
CAGCTAGCTG GACAGCCGGG GGAAGAGACC ATGCTGGAGA TGGACAAGTT CCCATACGTC 
GCGCTTTCAA AGACTTATAA CACCAACGCC CAGGTTGCCG ACAGCGCCGG CACCGCCACA 
GCCTACTTGT GTGGAGTGAA GGCCAACGAG GGGACAGTTG GGGTGAACGC AGCATCGGTG 
CGGGGTCAGT GCAACACCAC CAGGGGCAAT GAGGTGGACT CCATCTTGAA GTGGGCCAAA 
GAGGCAGGGA AATCCGTTGG CGTAGTGACC ACCACCCGGG TGAACCACGC AACCCCCAGC 
GCCGCCTACG CCCACTGCGT TAACAGAGAC TGGTTTTCGG ATAATGAGAT GCCCAAAGAA 
GCGGTAGAGG AGGGGTGCAG AGACATCGCC TGGCAGCTCA TATACAACGT TCCCGATATA 
GAGGTCATCA TGGGAGGGGG AAGAAAATAC ATGTTTCCTA AAAATACCCC CGACGTGGAG 
TACCCCAAGG AGGAGAAGGC CAACGGGACC AGGCTGGATG GATTAAACCT GACGAAGATC 
TGGCTGGAGA AGAAGCCACC TACGAAGAAT GCTCGTTACG TTTGGAACAG AGATCAGCTG 
ATGAACCTGA AACCACAAGA GGTGGACTAT CTCCTGGGTC TCTTTGAACC CAGCGATTTG 
GTGTATGAAC TGGACAGGAA CACCAGTACG GACCCCTCCC TGGTGGAGAT GGTGAAGGTG 
GCCATTGCTA TTCTGAAGAA AAACCCCAAA GGCTTCTTTC TCCTGGTGGA GGGTGGACGG 
ATAGACCATG GACACCACGA AGGAAAGGCC AAGCATGCTC TACATGAAGC TGTACAGATG 
GACATTGCCA TTGGTGTGGC CGGAGAGATG ACTTCCACCG AGGATACTCT GACCGTCGTC 
ACCGCTGACC ACTCCCACGT CTTCACATTC GGTGGATACA CCCATCGAGG AAACTCCATT 
TTCGGTTTAG CACCAACACT GAGTGACGTC GACCAGAAAC CTTTCACGTC CATCCTGTAT 
GGGAACGGTC CCGGCTACAA ACTAGTGGAC GGGAGCAGAG AGAACGTCTC CACTGTGGAC 
TATACTGATG CCAACTACCA CGCTCAGTCG GCGGTTCCCC TACGGATGGA GACCCACGGC 
GGAGAAGACG TAGCCGTATT TGCCAAGGGG CCCATGGCTC ATCTCCTGCA CGGTGTCCAC 
GAGCAGAATT ACATACCTCA CGTCATGGCA TACGCTTCCT GCGTCGGTCA GAATCAGAAC 
CACTGCAATT CCAGAAGCTC AGGCCACAGG ACCTCGGTCA CTCTGCTGCC CTCCGTCTTA 
TCGGTCCTCG CCGCACTGCG ATTGTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018429836.1
CDS	106..1515
Translation	MFLGDGMGVPTVTAARILKGQLAGQPGEETMLEMDKFPYVALSKTYNTNAQVADSAGTATAYLCGVKANEGTVGVNAASVRGQCNTTRGNEVDSILKWAKEAGKSVGVVTTTRVNHATPSAAYAHCVNRDWFSDNEMPKEAVEEGCRDIAWQLIYNVPDIEVIMGGGRKYMFPKNTPDVEYPKEEKANGTRLDGLNLTKIWLEKKPPTKNARYVWNRDQLMNLKPQEVDYLLGLFEPSDLVYELDRNTSTDPSLVEMVKVAIAILKKNPKGFFLLVEGGRIDHGHHEGKAKHALHEAVQMDIAIGVAGEMTSTEDTLTVVTADHSHVFTFGGYTHRGNSIFGLAPTLSDVDQKPFTSILYGNGPGYKLVDGSRENVSTVDYTDANYHAQSAVPLRMETHGGEDVAVFAKGPMAHLLHGVHEQNYIPHVMAYASCVGQNQNHCNSRSSGHRTSVTLLPSVLSVLAALRLL

Target ORF information:

RefSeq Version	XM_018574334.1
Organism	Nanorana parkeri
Definition	Nanorana parkeri alkaline phosphatase, liver/bone/kidney (ALPL), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018574334.1

ORF Insert Sequence:

ATGTTTCTCG GAGACGGGAT GGGGGTGCCA ACTGTCACTG CCGCTCGGAT CTTAAAGGGC 
CAGCTAGCTG GACAGCCGGG GGAAGAGACC ATGCTGGAGA TGGACAAGTT CCCATACGTC 
GCGCTTTCAA AGACTTATAA CACCAACGCC CAGGTTGCCG ACAGCGCCGG CACCGCCACA 
GCCTACTTGT GTGGAGTGAA GGCCAACGAG GGGACAGTTG GGGTGAACGC AGCATCGGTG 
CGGGGTCAGT GCAACACCAC CAGGGGCAAT GAGGTGGACT CCATCTTGAA GTGGGCCAAA 
GAGGCAGGGA AATCCGTTGG CGTAGTGACC ACCACCCGGG TGAACCACGC AACCCCCAGC 
GCCGCCTACG CCCACTGCGT TAACAGAGAC TGGTTTTCGG ATAATGAGAT GCCCAAAGAA 
GCGGTAGAGG AGGGGTGCAG AGACATCGCC TGGCAGCTCA TATACAACGT TCCCGATATA 
GAGGTCATCA TGGGAGGGGG AAGAAAATAC ATGTTTCCTA AAAATACCCC CGACGTGGAG 
TACCCCAAGG AGGAGAAGGC CAACGGGACC AGGCTGGATG GATTAAACCT GACGAAGATC 
TGGCTGGAGA AGAAGCCACC TACGAAGAAT GCTCGTTACG TTTGGAACAG AGATCAGCTG 
ATGAACCTGA AACCACAAGA GGTGGACTAT CTCCTGGGTC TCTTTGAACC CAGCGATTTG 
GTGTATGAAC TGGACAGGAA CACCAGTACG GACCCCTCCC TGGTGGAGAT GGTGAAGGTG 
GCCATTGCTA TTCTGAAGAA AAACCCCAAA GGCTTCTTTC TCCTGGTGGA GGGTGGACGG 
ATAGACCATG GACACCACGA AGGAAAGGCC AAGCATGCTC TACATGAAGC TGTACAGATG 
GACATTGCCA TTGGTGTGGC CGGAGAGATG ACTTCCACCG AGGATACTCT GACCGTCGTC 
ACCGCTGACC ACTCCCACGT CTTCACATTC GGTGGATACA CCCATCGAGG AAACTCCATT 
TTCGGTTTAG CACCAACACT GAGTGACGTC GACCAGAAAC CTTTCACGTC CATCCTGTAT 
GGGAACGGTC CCGGCTACAA ACTAGTGGAC GGGAGCAGAG AGAACGTCTC CACTGTGGAC 
TATACTGATG CCAACTACCA CGCTCAGTCG GCGGTTCCCC TACGGATGGA GACCCACGGC 
GGAGAAGACG TAGCCGTATT TGCCAAGGGG CCCATGGCTC ATCTCCTGCA CGGTGTCCAC 
GAGCAGAATT ACATACCTCA CGTCATGGCA TACGCTTCCT GCGTCGGTCA GAATCAGAAC 
CACTGCAATT CCAGAAGCTC AGGCCACAGG ACCTCGGTCA CTCTGCTGCC CTCCGTCTTA 
TCGGTCCTCG CCGCACTGCG ATTGTTGTGA